AR126240A1 - Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento - Google Patents

Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento

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Publication number
AR126240A1
AR126240A1 ARP220101666A ARP220101666A AR126240A1 AR 126240 A1 AR126240 A1 AR 126240A1 AR P220101666 A ARP220101666 A AR P220101666A AR P220101666 A ARP220101666 A AR P220101666A AR 126240 A1 AR126240 A1 AR 126240A1
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AR
Argentina
Prior art keywords
hect
upl
gene
e6ap
terminal
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ARP220101666A
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English (en)
Inventor
Pradeep Reddy Marri
Lolita George Mathew
Haejin Kim
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Pairwise Plants Services Inc
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Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR126240A1 publication Critical patent/AR126240A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Una planta o parte de ella que comprende al menos una mutación no natural en un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT) que codifica un polipéptido HECT E3 UPL. Una célula vegetal que comprende un sistema de edición, donde el sistema de edición comprende: (a) una proteína efectora CRISPR-Cas; y (b) un ácido nucleico guía que comprende una secuencia espaciadora con complementariedad con un gen objetivo endógeno que codifica un polipéptido HECT E3 UPL. Una célula vegetal que comprende al menos una mutación no natural en un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT), en donde la mutación es una sustitución, inserción, deleción o inversión que se introduce mediante un sistema de edición que comprende un dominio de unión de ácido nucleico que se une a un sitio objetivo en el gen endógeno HECT E3 UPL. Un método para generar variación en una región de un polipéptido de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT), que comprende: introducir un sistema de edición en una célula vegetal, en donde el sistema de edición está dirigido a una región de un gen HECT E3 UPL que codifica la región del polipéptido HECT E3 UPL. Un método para editar un sitio específico en el genoma de una célula vegetal, donde el método comprende: escindir, de manera específica del sitio, un sitio objetivo dentro de un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT) en la célula vegetal. Un ácido nucleico guía que se une a un sitio objetivo en un gen de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT). Un sistema de edición de genes que comprende una proteína efectora CRISPR-Cas en asociación con un ácido nucleico guía, en donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT). Un complejo que comprende un ácido nucleico guía y una proteína efectora CRISPR-Cas que comprende un dominio de escisión, en donde el ácido nucleico guía se une a un sitio objetivo en un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT). Un casete de expresión que comprende (a) un polinucleótido que codifica una proteína efectora CRISPR-Cas que comprende un dominio de escisión y (b) un ácido nucleico guía que se une a un sitio objetivo en un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT). Un ácido nucleico que codifica una mutación negativa dominante o una mutación nula de un polipéptido de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT), opcionalmente en donde el ácido nucleico comprende una secuencia de nucleótidos que tiene al menos un 90% de identidad de secuencia con cualquiera de SEQ ID Nº 119 - 125. Un polipéptido modificado de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT) que comprende una mutación en un residuo de aminoácido ubicado en la posición 1839 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 78, en la posición 1844 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 79, en la posición 1862 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 80, en la posición 1858 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 81, en la posición 1807 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 82, y/o en la posición 1800 con referencia a la numeración de posiciones de aminoácidos de SEQ ID Nº 83. Un método para crear una mutación en un gen endógeno de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT) en una planta. Un ácido nucleico guía que se une a un ácido nucleico objetivo en un gen de Proteína Ubiquitina Ligasa (UPL) E3 Homólogo a E6AP C-Terminal (HECT) que tiene el número de identificación de gen (ID del gen) de Zm00001d014920, Zm00001d004139, Glyma.11G107500, Glyma.12G032500, Glyma.06G003600 y/o Glyma.04G004000.
ARP220101666A 2021-06-24 2022-06-24 Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento AR126240A1 (es)

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US202163214498P 2021-06-24 2021-06-24

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US (1) US20230016618A1 (es)
EP (1) EP4359516A1 (es)
CN (1) CN117858945A (es)
AR (1) AR126240A1 (es)
BR (1) BR112023026845A2 (es)
CA (1) CA3224730A1 (es)
MX (1) MX2023014882A (es)
UY (1) UY39827A (es)
WO (1) WO2022271892A1 (es)

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