AR126362A1 - Métodos y composiciones para mejorar el desarrollo del sistema radicular - Google Patents
Métodos y composiciones para mejorar el desarrollo del sistema radicularInfo
- Publication number
- AR126362A1 AR126362A1 ARP220101734A ARP220101734A AR126362A1 AR 126362 A1 AR126362 A1 AR 126362A1 AR P220101734 A ARP220101734 A AR P220101734A AR P220101734 A ARP220101734 A AR P220101734A AR 126362 A1 AR126362 A1 AR 126362A1
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- Argentina
- Prior art keywords
- plant
- sequence
- nucleotide
- seq
- gene
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 14
- 125000003729 nucleotide group Chemical group 0.000 abstract 14
- LDEBVRIURYMKQS-WISUUJSJSA-N Ser-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H](N)CO LDEBVRIURYMKQS-WISUUJSJSA-N 0.000 abstract 8
- 108060006633 protein kinase Proteins 0.000 abstract 8
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 150000001413 amino acids Chemical group 0.000 abstract 5
- 230000035772 mutation Effects 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract 4
- 239000011574 phosphorus Substances 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102000001253 Protein Kinase Human genes 0.000 abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 239000012636 effector Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 230000034512 ubiquitination Effects 0.000 abstract 2
- 238000010798 ubiquitination Methods 0.000 abstract 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 108091026890 Coding region Proteins 0.000 abstract 1
- 238000010362 genome editing Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4678—Triticum sp. [wheat]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/11—Protein-serine/threonine kinases (2.7.11)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/11—Protein-serine/threonine kinases (2.7.11)
- C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a composiciones y métodos para modificar la arquitectura radicular en una planta a través de la modificación de un gen endógeno de la proteína quinasa Ser-Thr, como los ácidos nucleicos de Tolerancia a la Falta de Fósforo 1 (PSTOL 1) endógenos. La invención además se refiere a plantas producidas usando los métodos y composiciones de la invención. Reivindicación 1: Una planta o parte de planta de la misma que comprende por lo menos una mutación no natural en un gen endógeno de la proteína quinasa Ser-Thr que se expresa en las raíces de la planta o parte de la misma, en donde el gen endógeno de la proteína quinasa Ser-Thr que comprende la por lo menos única mutación no natural codifica una proteína quinasa Ser-Thr, opcionalmente que tiene una mayor estabilidad. Reivindicación 2: La planta o parte de planta de la misma de la reivindicación 1, en donde el gen endógeno de la proteína quinasa Ser-Thr es un gen endógeno de Tolerancia a la Falta de Fósforo 1 (PSTOL1) que codifica un polipéptido PSTOL1. Reivindicación 3: La planta o parte de planta de la misma de la reivindicación 1 o la reivindicación 2, en donde la por lo menos única mutación no natural está en una región del gen endógeno de la proteína quinasa Ser-Thr que codifica un sitio de ubiquitinación en la proteína quinasa Ser-Thr o el polipéptido PSTOL1. Reivindicación 4: La planta o parte de planta de la misma de la reivindicación 3, en donde el sitio de ubiquitinación es un motivo PEST (P-prolina, E-glutamina, S-serina, T-treonina) en la proteína quinasa Ser-Thr o el polipéptido PSTOL1. Reivindicación 5: La planta o parte de planta de la misma de cualquiera de las reivindicaciones 1 - 4, en donde la por lo menos única mutación no natural da como resultado una planta con una arquitectura radicular mejorada, en donde la arquitectura radicular está mejorada en comparación con una planta o parte de planta de control que no comprende la misma mutación. Reivindicación 6: La planta o parte de planta de la misma de la reivindicación 5, en donde la arquitectura radicular mejorada se caracteriza por uno o más de los siguientes fenotipos de ángulo de raíz más pronunciado de las raíces primarias, laterales y/o secundarias, raíces más largas, mayor número de ramas, aumento del aerénquima, y/o aumento de la biomasa radicular. Reivindicación 7: La planta o parte de planta de la misma de la reivindicación 5 o la reivindicación 6, en donde la planta que tiene la arquitectura radicular mejorada exhibe rasgos de rendimiento mejorados y/o rasgos de rendimiento que son retenidos bajo condiciones de estrés. Reivindicación 59: Un sistema de edición de genes que comprende una proteína efectora CRISPR-Cas asociada con un ácido nucleico guía, en donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen de Tolerancia a la Falta de Fósforo 1 (PSTOL 1). Reivindicación 67: Un complejo que comprende una proteína efectora CRISPR-Cas que comprende un dominio de escisión y un ácido nucleico guía, en donde el ácido nucleico guía se une a un sitio diana en un gen de Tolerancia a la Falta de Fósforo 1 (PSTOL 1), el gen PSTOL1: (a) comprende una secuencia que tiene por lo menos 80% de identidad de secuencia con la secuencia de nucleótidos de la SEQ ID Nº 72; (b) comprende una secuencia de codificación que tiene por lo menos 80% de identidad de secuencia con la secuencia de nucleótidos de la SEQ ID Nº 73; (c) comprende una secuencia de nucleótidos que tiene por lo menos 80% de identidad de secuencia con la una región de nucleótidos consecutivos de la SEQ ID Nº 72 localizada desde aproximadamente el nucleótido 3106 a aproximadamente el nucleótido 3234 o desde aproximadamente el nucleótido 3125 a aproximadamente el nucleótido 3214, o una secuencia de nucleótidos que tiene por lo menos 80% de identidad de secuencia con la una región de nucleótidos consecutivos de la SEQ ID Nº 73 localizada desde aproximadamente el nucleótido 935 a aproximadamente el nucleótido 1024, opcionalmente una secuencia de nucleótidos que tiene por lo menos 80% de identidad de secuencia con la una región de nucleótidos consecutivos de la SEQ ID Nº 75 o la SEQ ID Nº 76; (d) codifica una secuencia de polipéptidos que tiene por lo menos 80% de identidad con la secuencia de aminoácidos de la SEQ ID Nº 74; y/o (e) codifica una secuencia de aminoácidos que tiene una región de aminoácidos consecutivos con por lo menos 80% de identidad con la región de la SEQ ID Nº 74 localizada entre aproximadamente el residuo 316 y el residuo 344, opcionalmente codifica una secuencia de aminoácidos que tiene una región con 80% de identidad con la secuencia de aminoácidos de la SEQ ID Nº 77, en donde el dominio de escisión escinde una cadena diana en el gen PSTOL1.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163217332P | 2021-07-01 | 2021-07-01 |
Publications (1)
Publication Number | Publication Date |
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AR126362A1 true AR126362A1 (es) | 2023-10-11 |
Family
ID=82786843
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP220101734A AR126362A1 (es) | 2021-07-01 | 2022-07-01 | Métodos y composiciones para mejorar el desarrollo del sistema radicular |
Country Status (7)
Country | Link |
---|---|
US (1) | US20230027468A1 (es) |
EP (1) | EP4362663A1 (es) |
CN (1) | CN117794358A (es) |
AR (1) | AR126362A1 (es) |
CA (1) | CA3224982A1 (es) |
UY (1) | UY39836A (es) |
WO (1) | WO2023278651A1 (es) |
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-
2022
- 2022-06-30 CN CN202280055125.7A patent/CN117794358A/zh active Pending
- 2022-06-30 WO PCT/US2022/035641 patent/WO2023278651A1/en active Application Filing
- 2022-06-30 CA CA3224982A patent/CA3224982A1/en active Pending
- 2022-06-30 US US17/853,997 patent/US20230027468A1/en active Pending
- 2022-06-30 EP EP22750945.2A patent/EP4362663A1/en active Pending
- 2022-07-01 AR ARP220101734A patent/AR126362A1/es unknown
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CN117794358A (zh) | 2024-03-29 |
WO2023278651A1 (en) | 2023-01-05 |
EP4362663A1 (en) | 2024-05-08 |
CA3224982A1 (en) | 2023-01-05 |
US20230027468A1 (en) | 2023-01-26 |
UY39836A (es) | 2023-01-31 |
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