CL2022001482A1 - Gen cca para la resistencia a los virus - Google Patents

Gen cca para la resistencia a los virus

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Publication number
CL2022001482A1
CL2022001482A1 CL2022001482A CL2022001482A CL2022001482A1 CL 2022001482 A1 CL2022001482 A1 CL 2022001482A1 CL 2022001482 A CL2022001482 A CL 2022001482A CL 2022001482 A CL2022001482 A CL 2022001482A CL 2022001482 A1 CL2022001482 A1 CL 2022001482A1
Authority
CL
Chile
Prior art keywords
seq
gene
cca
nucleotide sequence
addition enzyme
Prior art date
Application number
CL2022001482A
Other languages
English (en)
Inventor
Kalisvaart Jonathan
Jacobus Johannes Maria Frijters Raoul
Johannes Wilhelmus LUDEKING Daniël
Johannes Marinus Roovers Alwin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of CL2022001482A1 publication Critical patent/CL2022001482A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07072—CCA tRNA nucleotidyltransferase (2.7.7.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere a un gen CCA modificado que codifica una enzima de adición de CCA, cuyo gen CCA modificado conduce a la resistencia contra un virus de ARN de cadena positiva que tiene una TLS, en donde el gen CCA modificado se selecciona del grupo que consiste en: un gen que comprende una secuencia de nucleótidos que codifica una enzima de adición de CCA de conformidad con SEC ID No. 8, SEC ID No. 9, SEC ID No. 10, o SEC ID No. 11; un gen que comprende una secuencia promotora que comprende SEC ID No. 12, SEC ID No. 13, SEC ID No. 14, SEC ID No. 15, o SEC ID No. 16; un gen que comprende una secuencia de nucleótidos que codifica una enzima de adición de CCA que tiene una supresión, una sustitución, o una inserción de al menos un aminoácido en comparación con SEC ID No. 2 o SEC ID No. 7; un gen que comprende una secuencia de nucleótidos que codifica una enzima de adición de CCA con una supresión, una sustitución o una inserción de al menos un aminoácido en comparación con SEC ID No. 8, SEC ID No. 9, SEC ID No. 10 o SEC ID No. 11; un gen que comprende una secuencia de nucleótidos que codifica una enzima de adición de CCA que tiene al menos un 80% de identidad de secuencia con SEC ID No. 8, SEC ID No. 9, SEC ID No. 10, o SEC ID No. 11; y un gen que comprende una secuencia promotora que tiene al menos un 80% de identidad de secuencia con SEC ID No. 12, SEC ID No. 13, SEC ID No. 14, SEC ID No. 15, o SEC ID No. 16. La invención se refiere además a plantas y semillas que comprenden los genes modificados, métodos para hacer e identificar tales plantas y para usar el gen.
CL2022001482A 2019-12-04 2022-06-03 Gen cca para la resistencia a los virus CL2022001482A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EPPCT/EP2019/083733 2019-12-04

Publications (1)

Publication Number Publication Date
CL2022001482A1 true CL2022001482A1 (es) 2023-01-27

Family

ID=68886998

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2022001482A CL2022001482A1 (es) 2019-12-04 2022-06-03 Gen cca para la resistencia a los virus

Country Status (11)

Country Link
US (1) US20220403409A1 (es)
EP (1) EP4069853A1 (es)
JP (1) JP2023504713A (es)
CN (1) CN114929881A (es)
AU (1) AU2020396217A1 (es)
CA (1) CA3159997A1 (es)
CL (1) CL2022001482A1 (es)
IL (1) IL293400A (es)
MA (1) MA56910B1 (es)
MX (1) MX2022006616A (es)
WO (1) WO2021110855A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4181663A1 (en) 2020-07-17 2023-05-24 Rijk Zwaan Zaadteelt en Zaadhandel B.V. Gene leading to tobrfv resistance in s. lycopersicum
WO2023156569A1 (en) 2022-02-17 2023-08-24 Syngenta Crop Protection Ag Novel tomato plants with tobrfv resistance

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2914151T3 (es) * 2015-03-04 2022-06-07 Duemmen Group Bv Gen de resistencia al moho en Kalanchoe

Also Published As

Publication number Publication date
WO2021110855A1 (en) 2021-06-10
MX2022006616A (es) 2022-06-24
MA56910A1 (fr) 2023-02-28
EP4069853A1 (en) 2022-10-12
MA56910B1 (fr) 2023-09-27
US20220403409A1 (en) 2022-12-22
IL293400A (en) 2022-07-01
CN114929881A (zh) 2022-08-19
AU2020396217A1 (en) 2022-06-16
JP2023504713A (ja) 2023-02-06
CA3159997A1 (en) 2021-06-10

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