AR126924A1 - Modificación de genes de peptidasa de unión a ubiquitina en plantas para mejorar rasgos de rendimiento - Google Patents
Modificación de genes de peptidasa de unión a ubiquitina en plantas para mejorar rasgos de rendimientoInfo
- Publication number
- AR126924A1 AR126924A1 ARP220102344A ARP220102344A AR126924A1 AR 126924 A1 AR126924 A1 AR 126924A1 AR P220102344 A ARP220102344 A AR P220102344A AR P220102344 A ARP220102344 A AR P220102344A AR 126924 A1 AR126924 A1 AR 126924A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- nucleic acid
- gene
- sequence
- identity
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 9
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 abstract 16
- 108020004707 nucleic acids Proteins 0.000 abstract 16
- 150000007523 nucleic acids Chemical class 0.000 abstract 16
- 108090000623 proteins and genes Proteins 0.000 abstract 11
- 101150008235 DA1 gene Proteins 0.000 abstract 8
- 102000035195 Peptidases Human genes 0.000 abstract 8
- 102000044159 Ubiquitin Human genes 0.000 abstract 8
- 108090000848 Ubiquitin Proteins 0.000 abstract 8
- 235000019833 protease Nutrition 0.000 abstract 8
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 5
- 239000002773 nucleotide Substances 0.000 abstract 5
- 125000003729 nucleotide group Chemical group 0.000 abstract 5
- 239000012636 effector Substances 0.000 abstract 4
- 238000010362 genome editing Methods 0.000 abstract 4
- 230000035772 mutation Effects 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 125000006850 spacer group Chemical group 0.000 abstract 3
- 108091007494 Nucleic acid- binding domains Proteins 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
- A01H6/542—Glycine max [soybean]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Medicinal Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La invención está relacionada con composiciones y métodos para modificar genes de Peptidasa de Unión a Ubiquitina (DA1) en plantas, opcionalmente para mejorar rasgos de rendimiento. La invención además está relacionada con plantas con rasgos de rendimiento mejorados aumentados producidas con el uso de los métodos y las composiciones de la invención. Reivindicación 1: Una planta o una parte de esa planta que comprende al menos una mutación en un gen endógeno de Peptidasa de Unión a Ubiquitina (DA1) que codifica un polipéptido de peptidasa de unión a ubiquitina (DA1). Reivindicación 35: Una célula vegetal que comprende al menos una mutación dentro de un gen endógeno de Peptidasa de Unión a Ubiquitina (DA1), donde la al menos una mutación es una sustitución, inserción o deleción que se introduce usando un sistema de edición que comprende un dominio de unión a ácido nucleico que se une a un sitio diana en el gen endógeno DA1. Reivindicación 69: Un método para producir una planta, que comprende: a) contactar una población de células vegetales que comprende un gen endógeno de Peptidasa de Unión a Ubiquitina (DA1) con una nucleasa enlazada con un dominio de unión a ácido nucleico (por ejemplo, sistema de edición) que se une a una secuencia i) que tiene al menos 80% de identidad de secuencia con una secuencia de nucleótidos de cualquiera de SEQ ID Nº 69, 70, 109, o 110, ii) que comprende una región que tiene al menos 90% de identidad con cualquiera de SEQ ID Nº 72 - 101 o 112 - 139; iii) que codifica una secuencia de aminoácidos que tiene al menos 80% de identidad de secuencia con SEQ ID Nº 71 o SEQ ID Nº 111, y/o iv) que codifica una región que tiene al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 102 - 108 o 140 - 146, y/o; b) seleccionar una célula vegetal de la población de células vegetales en las cuales se ha mutado un gen endógeno DA1, con lo cual se produce una célula vegetal que comprende una mutación en el gen endógeno DA1; y c) desarrollar la célula vegetal seleccionada en una planta. Reivindicación 90: Un ácido nucleico guía que se une a un sitio diana en un gen de Peptidasa de Unión a Ubiquitina (DA1), donde el sitio diana está en una región del gen DA1 que tiene al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 72 - 101 o 112 - 139, opcionalmente una región del gen DA1 que tiene al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 74 - 76, 78 - 80, 82 - 85, 89 - 96, 99 - 101, 114 - 116, 119, 120, 123 - 126, 128 - 131, 133 - 135, 138, o 139, opcionalmente al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 82 - 85, 99 - 101, 123 - 126, 138, o 139. Reivindicación 91: El ácido nucleico guía de la reivindicación 90, donde el ácido nucleico guía comprende un espaciador que comprende una secuencia de nucleótidos de cualquiera de SEQ ID Nº 147 - 153. Reivindicación 93: El sistema de la reivindicación 92 [sic] que además comprende un ácido nucleico tracr que se asocia con el ácido nucleico guía y una proteína efectora CRISPR-Cas, opcionalmente donde el ácido nucleico tracr y el ácido nucleico guía están covalentemente enlazados. Reivindicación 94: Un sistema de edición génica que comprende una proteína efectora CRISPR-Cas en asociación con un ácido nucleico guía, donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen endógeno de Peptidasa de Unión a Ubiquitina (DA1). Reivindicación 95: El sistema de edición génica de la reivindicación 94, donde el gen DA1: a) comprende una secuencia que tiene al menos 80% de identidad de secuencia con una secuencia de nucleótidos de cualquiera de SEQ ID Nº 69, 70, 109 o 110; b) comprende una región que tiene al menos 90% de identidad con cualquiera de SEQ ID Nº 72 - 101 o SEQ ID Nº 112 - 139; c) codifica una secuencia de aminoácidos que tiene al menos 80% de identidad de secuencia con SEQ ID Nº 71 o SEQ ID Nº 111; y/o d) codifica una secuencia de aminoácidos que comprende una región que tiene al menos 90% de identidad con cualquiera de SEQ ID Nº 102 - 108 o 140 - 146. Reivindicación 96: El sistema de edición génica de la reivindicación 94 o la reivindicación 95, donde el ácido nucleico guía comprende una secuencia espaciadora que comprende una secuencia de nucleótidos de cualquiera de SEQ ID Nº 147 - 153. Reivindicación 97: El sistema de edición génica de cualquiera de las reivindicaciones 94 - 96, que además comprende un ácido nucleico tracr que se asocia con el ácido nucleico guía y una proteína efectora CRISPR-Cas, opcionalmente donde el ácido nucleico tracr y el ácido nucleico guía están covalentemente enlazados. Reivindicación 98: Un complejo que comprende un ácido nucleico guía y una proteína efectora CRISPR-Cas que comprende un dominio de escisión, donde el ácido nucleico guía se une a un sitio diana en un gen endógeno de Peptidasa de Unión a Ubiquitina (DA1), donde el gen endógeno DA1: a) comprende una secuencia que tiene al menos 80% de identidad de secuencia con una secuencia de nucleótidos de cualquiera de SEQ ID Nº 69, 70, 109 o 110; b) comprende una región que tiene al menos 90% de identidad con cualquiera de SEQ ID Nº 72 - 101 o 112 - 139; c) codifica una secuencia de aminoácidos que tiene al menos 80% de identidad de secuencia con SEQ ID Nº 71 o SEQ ID Nº 111; y/o d) codifica una secuencia de aminoácidos que comprende una región que tiene al menos 90% de identidad con cualquiera de SEQ ID Nº 102 - 108 o 140 - 146, y el dominio de escisión escinde una hebra diana en el gen DA1.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163238394P | 2021-08-30 | 2021-08-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR126924A1 true AR126924A1 (es) | 2023-11-29 |
Family
ID=83438315
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP220102344A AR126924A1 (es) | 2021-08-30 | 2022-08-30 | Modificación de genes de peptidasa de unión a ubiquitina en plantas para mejorar rasgos de rendimiento |
Country Status (4)
Country | Link |
---|---|
US (1) | US20230074699A1 (es) |
AR (1) | AR126924A1 (es) |
CA (1) | CA3230167A1 (es) |
WO (1) | WO2023034731A1 (es) |
Family Cites Families (121)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2010A (en) | 1841-03-18 | Machine foe | ||
US24077A (en) | 1859-05-17 | Window-sash supporter | ||
US137395A (en) | 1873-04-01 | Improvement in nuts | ||
US2009A (en) | 1841-03-18 | Improvement in machines for boring war-rockets | ||
NZ221259A (en) | 1986-07-31 | 1990-05-28 | Calgene Inc | Seed specific transcriptional regulation |
US6040504A (en) | 1987-11-18 | 2000-03-21 | Novartis Finance Corporation | Cotton promoter |
ATE112314T1 (de) | 1988-05-17 | 1994-10-15 | Lubrizol Genetics Inc | Pflanzliches ubiquitinpromotorsystem. |
US5641876A (en) | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
EP0452269B1 (en) | 1990-04-12 | 2002-10-09 | Syngenta Participations AG | Tissue-preferential promoters |
US6395966B1 (en) | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
US5459252A (en) | 1991-01-31 | 1995-10-17 | North Carolina State University | Root specific gene promoter |
DK0600993T3 (da) | 1991-08-27 | 2000-05-08 | Novartis Ag | Proteiner medinsektdræbende egenskaber mod Homoptera-insekter samt anvendelse deraf til plantebeskyttelse |
UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
BR9604993B1 (pt) | 1995-04-20 | 2009-05-05 | dna mutante codificando uma proteìna ahas mutante de sìntese de ácido acetohidróxi e proteìnas ahas mutantes. | |
MX9705101A (es) | 1995-11-06 | 1997-10-31 | Wisconsin Alumni Res Found | Toxinas proteinicas insecticidas obtenidas a partir de photorhabdus luminescens. |
EP0970185A4 (en) | 1996-08-29 | 2003-02-26 | Dowelanco | INSECTICIDE PROTEIN TOXINS ISOLATED FROM PHOTORHABDUSES |
BR9808475A (pt) | 1997-04-03 | 2000-05-23 | Dekalb Genetics Corp | Linhagens de milho resistentes à glifosato. |
CN1137137C (zh) | 1997-05-05 | 2004-02-04 | 道农业科学公司 | 来自致病杆菌的杀昆虫蛋白毒素 |
JP2002504335A (ja) | 1998-02-20 | 2002-02-12 | シンジェンタ リミテッド | 花粉特異的プロモーター |
US6333449B1 (en) | 1998-11-03 | 2001-12-25 | Plant Genetic Systems, N.V. | Glufosinate tolerant rice |
AU1336200A (en) | 1998-11-03 | 2000-05-22 | Aventis Cropscience N.V. | Glufosinate tolerant rice |
US6509516B1 (en) | 1999-10-29 | 2003-01-21 | Plant Genetic Systems N.V. | Male-sterile brassica plants and methods for producing same |
US6506963B1 (en) | 1999-12-08 | 2003-01-14 | Plant Genetic Systems, N.V. | Hybrid winter oilseed rape and methods for producing same |
DE60041505D1 (de) | 1999-12-28 | 2009-03-19 | Bayer Bioscience Nv | Inzektizide proteinen von bacillus thuringiensis |
US6395485B1 (en) | 2000-01-11 | 2002-05-28 | Aventis Cropscience N.V. | Methods and kits for identifying elite event GAT-ZM1 in biological samples |
MXPA02009335A (es) | 2000-03-27 | 2003-02-12 | Syngenta Participations Ag | Promotores del virus de la rizadura amarilla del cestrum. |
AR044724A1 (es) | 2000-03-27 | 2005-10-05 | Syngenta Participations Ag | Promotores del virus de la rizadura amarilla del cestrum |
BR122013026754B1 (pt) | 2000-06-22 | 2018-02-27 | Monsanto Company | Molécula de dna e processos para produzir uma planta de milho tolerante à aplicação do herbicida glifosato |
US6713259B2 (en) | 2000-09-13 | 2004-03-30 | Monsanto Technology Llc | Corn event MON810 and compositions and methods for detection thereof |
US6689880B2 (en) | 2000-09-29 | 2004-02-10 | Monsanto Technology Llc | DNA molecule for detecting glyphosate tolerant wheat plant 33391 and progeny thereof |
AU1536302A (en) | 2000-10-25 | 2002-05-06 | Monsanto Technology Llc | Cotton event pv-ghgt07(1445) and compositions and methods for detection thereof |
ATE509110T1 (de) | 2000-10-30 | 2011-05-15 | Monsanto Technology Llc | Canola event pv-bngt(rt73), zusammensetzungen und verfahren zum nachweis davon |
AU2002236442A1 (en) | 2000-11-20 | 2002-05-27 | Monsanto Technology Llc | Cotton event pv-ghbk04 (531) and compositions and methods for detection thereof |
AU2002218413A1 (en) | 2000-11-30 | 2002-06-11 | Ses Europe N.V. | Glyphosate resistant transgenic sugar beet characterised by a specific transgene insertion (t227-1), methods and primers for the detection of said insertion |
EG26529A (en) | 2001-06-11 | 2014-01-27 | مونسانتو تكنولوجى ل ل سى | Prefixes for detection of DNA molecule in cotton plant MON15985 which gives resistance to damage caused by insect of squamous lepidoptera |
US6818807B2 (en) | 2001-08-06 | 2004-11-16 | Bayer Bioscience N.V. | Herbicide tolerant cotton plants having event EE-GH1 |
AR037856A1 (es) | 2001-12-17 | 2004-12-09 | Syngenta Participations Ag | Evento de maiz |
WO2004011601A2 (en) | 2002-07-29 | 2004-02-05 | Monsanto Technology, Llc | Corn event pv-zmir13 (mon863) plants and compositions and methods for detection thereof |
GB0225129D0 (en) | 2002-10-29 | 2002-12-11 | Syngenta Participations Ag | Improvements in or relating to organic compounds |
CA2508032C (en) | 2002-12-05 | 2014-09-16 | Monsanto Technology Llc | Bentgrass event asr-368 and compositions and methods for detection thereof |
ES2382804T3 (es) | 2003-02-12 | 2012-06-13 | Monsanto Technology Llc | Evento de algodón MON 88913 y composiciones y procedimientos para su detección |
US7335816B2 (en) | 2003-02-28 | 2008-02-26 | Kws Saat Ag | Glyphosate tolerant sugar beet |
EP1597373B1 (de) | 2003-02-20 | 2012-07-18 | KWS Saat AG | Glyphosat-tolerante Zuckerrübe |
WO2004099447A2 (en) | 2003-05-02 | 2004-11-18 | Dow Agrosciences Llc | Corn event tc1507 and methods for detection thereof |
EP1687429B1 (en) | 2003-10-06 | 2011-01-12 | Syngenta Participations AG | Promoter functional in plant plastids |
KR100537955B1 (ko) | 2003-10-29 | 2005-12-20 | 학교법인고려중앙학원 | 꽃가루 특이적 유전자 발현 프로모터 |
BRPI0416472A (pt) | 2003-12-01 | 2007-03-06 | Syngenta Participations Ag | plantas de algodão resistentes a insetos e métodos de detecção das mesmas |
WO2005054480A2 (en) | 2003-12-01 | 2005-06-16 | Syngenta Participations Ag | Insect resistant cotton plants and methods of detecting the same |
US7157281B2 (en) | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
CA2547563C (en) | 2003-12-15 | 2014-02-18 | Monsanto Technology Llc | Corn plant mon88017 and compositions and methods for detection thereof |
GB0402106D0 (en) | 2004-01-30 | 2004-03-03 | Syngenta Participations Ag | Improved fertility restoration for ogura cytoplasmic male sterile brassica and method |
CN1933724B (zh) | 2004-03-25 | 2012-06-06 | 辛根塔参与股份公司 | Mir604 转基因玉米植物细胞 |
EP2333082B1 (en) | 2004-03-26 | 2015-01-07 | Dow AgroSciences LLC | Cry1F and Cry1AC transgenic cotton lines and event-specific identification thereof |
WO2006039376A2 (en) | 2004-09-29 | 2006-04-13 | Pioneer Hi-Bred International, Inc. | Corn event das-59122-7 and methods for detection thereof |
MX2007010036A (es) | 2005-03-16 | 2007-10-04 | Syngenta Participations Ag | Maiz evento 3272 y metodos para la deteccion del mismo. |
CA2603944C (en) | 2005-04-08 | 2015-06-23 | Bayer Bioscience N.V. | Elite event a2704-12 comprising the integration of the phosphinothricin acetyltransferase (pat) gene into soybeans, and methods and kits for identifying such event in biological samples |
BRPI0610462B1 (pt) | 2005-04-11 | 2018-02-27 | Bayer Cropscience Nv | Métodos para identificar o evento elite a5547-127 em amostras biológicas, para confirmar a pureza de semente e para triar sementes para a presença do referido evento, kits e sondas para a realização dos referidos métodos, bem como dna referente ao evento elite a5547-127 |
PT1885176T (pt) | 2005-05-27 | 2016-11-28 | Monsanto Technology Llc | Evento mon89788 de soja e métodos para a sua deteção |
CN101184847B (zh) | 2005-06-02 | 2015-02-25 | 先正达参股股份有限公司 | 表达cry1ab的杀昆虫转基因棉ce43-67b |
WO2006128568A2 (en) | 2005-06-02 | 2006-12-07 | Syngenta Participations Ag | T342-142, insecticidal transgenic cotton expressing cry1ab |
EP1917358A2 (en) | 2005-06-02 | 2008-05-07 | Syngeta Participations AG | Ce44-69d insecticidal transgenic cotton expressing cry1ab |
WO2006128569A2 (en) | 2005-06-02 | 2006-12-07 | Syngenta Participations Ag | 1143-14a, insecticidal transgenic cotton expressing cry1ab |
WO2006128570A1 (en) | 2005-06-02 | 2006-12-07 | Syngenta Participations Ag | 1143-51b insecticidal cotton |
WO2006128572A1 (en) | 2005-06-02 | 2006-12-07 | Syngenta Participations Ag | Ce46-02a insecticidal cotton |
MX2008001839A (es) | 2005-08-08 | 2008-04-09 | Bayer Bioscience Nv | Plantas de algodon con tolerancia a herbicidas y metodos para identificar las mismas. |
KR20080052606A (ko) | 2005-08-24 | 2008-06-11 | 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 | 다수 제초제에 대해 내성을 제공하는 조성물 및 이의 이용방법 |
WO2011066360A1 (en) | 2009-11-24 | 2011-06-03 | Dow Agrosciences Llc | Detection of aad-12 soybean event 416 |
CN101421404A (zh) | 2006-02-10 | 2009-04-29 | 马哈拉施特拉杂交种子有限公司 | 表达cry1ac基因的转基因茄子(solanum melongena) |
EP2021476B1 (en) | 2006-05-26 | 2014-07-09 | Monsanto Technology, LLC | Corn plant and seed corresponding to transgenic event mon89034 and methods for detection and use thereof |
KR20130020849A (ko) | 2006-06-03 | 2013-02-28 | 신젠타 파티서페이션즈 아게 | 옥수수 이벤트 mir162 |
US7951995B2 (en) | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
US7928295B2 (en) | 2006-08-24 | 2011-04-19 | Bayer Bioscience N.V. | Herbicide tolerant rice plants and methods for identifying same |
US20080064032A1 (en) | 2006-09-13 | 2008-03-13 | Syngenta Participations Ag | Polynucleotides and uses thereof |
US7928296B2 (en) | 2006-10-30 | 2011-04-19 | Pioneer Hi-Bred International, Inc. | Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof |
EP2078089B1 (en) | 2006-10-31 | 2016-05-04 | E. I. du Pont de Nemours and Company | Soybean event dp-305423-1 and compositions and methods for the identification and/or detection thereof |
WO2008114282A2 (en) | 2007-03-19 | 2008-09-25 | Maharashtra Hybrid Seeds Company Limited | Transgenic rice (oryza sativa) comprising pe-7 event and method of detection thereof |
AU2008235035B2 (en) | 2007-04-05 | 2013-10-10 | BASF Agricultural Solutions Seed US LLC | Insect resistant cotton plants and methods for identifying same |
EP2615173B1 (en) | 2007-06-11 | 2020-09-16 | Basf Agricultural Solutions Seed Us Llc | Insect resistant cotton plants and methods for identifying same |
GB0719919D0 (en) * | 2007-10-11 | 2007-11-21 | Plant Bioscience Ltd | Control of plant seed and organ size |
MX2010005352A (es) | 2007-11-15 | 2010-07-02 | Monsanto Technology Llc | Plantas y semillas de soya correspondientes al evento transgenico mon87701 y metodos de deteccion del mismo. |
WO2009100188A2 (en) | 2008-02-08 | 2009-08-13 | Dow Agrosciences Llc | Methods for detection of corn event das-59132 |
CN104805115A (zh) | 2008-02-14 | 2015-07-29 | 先锋国际良种公司 | Spt事件侧翼的植物基因组dna及用于鉴定spt事件的方法 |
MX2010008928A (es) | 2008-02-15 | 2010-09-09 | Monsanto Technology Llc | Planta de soya y semilla que corresponde al evento transgenico mon87769 y metodos para deteccion del mismo. |
DK2247736T3 (da) | 2008-02-29 | 2013-08-05 | Monsanto Technology Llc | Majsplanteevent mon87460 og sammensætninger og fremgangsmåder til detektion deraf |
EP2299804A4 (en) | 2008-06-11 | 2011-05-18 | Dow Agrosciences Llc | RECOMBINANT PRODUCTS FOR EXPRESSION OF HERBICIDE TOLERANT GENES, ASSOCIATED PLANTS, AND COMBINATIONS OF ASSOCIATED CHARACTERS |
WO2010024976A1 (en) | 2008-08-29 | 2010-03-04 | Monsanto Technology Llc | Soybean plant and seed corresponding to transgenic event mon87754 and methods for detection thereof |
UY32145A (es) | 2008-09-29 | 2010-04-30 | Monsanto Technology Llc | Evento transgénico de soja mon87705 y métodos para detectar el mismo |
MX348698B (es) | 2008-12-16 | 2017-06-26 | Syngenta Participations Ag | Evento 5307 del maiz. |
UA106061C2 (uk) | 2008-12-19 | 2014-07-25 | Сінгента Партісіпейшнс Аг | Трансгенний варіант цукрового буряку gm rz13 |
WO2010080829A1 (en) | 2009-01-07 | 2010-07-15 | Basf Agrochemical Products B.V. | Soybean event 127 and methods related thereto |
US8618360B2 (en) | 2009-03-30 | 2013-12-31 | Monsanto Technology Llc | Rice transgenic event 17314 and methods of use thereof |
WO2010117737A1 (en) | 2009-03-30 | 2010-10-14 | Monsanto Technology Llc | Rice transgenic event17053 and methods of use thereof |
EP2467010B1 (en) | 2009-08-19 | 2018-11-28 | Dow AgroSciences LLC | Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof |
CN102596984A (zh) | 2009-09-17 | 2012-07-18 | 孟山都技术公司 | 大豆转基因事件mon 87708及其使用方法 |
MX2012005932A (es) | 2009-11-23 | 2012-09-12 | Bayer Cropscience Nv | Plantas de soya tolerantes a herbicidas y metodos para indentificar las mismas. |
CA2780448C (en) | 2009-11-23 | 2018-02-13 | Monsanto Technology Llc | Transgenic maize event mon 87427 and the relative development scale |
US8581046B2 (en) | 2010-11-24 | 2013-11-12 | Pioneer Hi-Bred International, Inc. | Brassica gat event DP-073496-4 and compositions and methods for the identification and/or detection thereof |
CN104928256A (zh) | 2009-11-24 | 2015-09-23 | 陶氏益农公司 | Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定 |
US20110154526A1 (en) | 2009-12-17 | 2011-06-23 | Pioneer Hi-Bred International, Inc. | Maize event DP-043A47-3 and methods for detection thereof |
WO2011084632A1 (en) | 2009-12-17 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Maize event dp-032316-8 and methods for detection thereof |
ES2730684T3 (es) | 2009-12-17 | 2019-11-12 | Pioneer Hi Bred Int | Evento de maíz DP-004114-3 y métodos para la detección del mismo |
US20110154525A1 (en) | 2009-12-17 | 2011-06-23 | Pioneer Hi-Bred International, Inc. | Maize event DP-040416-8 and methods for detection thereof |
JP5957447B2 (ja) | 2010-06-04 | 2016-07-27 | モンサント テクノロジー エルエルシー | 遺伝子組換えアブラナ事象mon88302および同使用方法 |
WO2012033794A2 (en) | 2010-09-08 | 2012-03-15 | Dow Agrosciences Llc | Aad-12 event 1606 and related transgenic soybean lines |
MX346994B (es) | 2010-10-12 | 2017-04-06 | Monsanto Technology Llc | Planta y semilla de soja correspondiente al evento transgénico mon87712 y métodos para su detección. |
WO2012071039A1 (en) | 2010-11-24 | 2012-05-31 | Pioner Hi-Bred International, Inc. | Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof |
CA2819684C (en) | 2010-12-03 | 2024-05-07 | Dow Agrosciences Llc | Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof |
BR112013015745B1 (pt) | 2010-12-03 | 2021-01-19 | Ms Technologies, Llc | polinucleotídeos referente ao evento de tolerância a herbicida 8291.45.36.2, cassete de expressão, sonda, bem como processos para identificação do evento, determinação da zigosidade, produção de uma planta de soja transgênica e produção de uma proteína em uma célula de planta |
TWI667347B (zh) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種syht0h2及偵測其之組合物及方法 |
WO2012134808A1 (en) | 2011-03-30 | 2012-10-04 | Monsanto Technology Llc | Cotton transgenic event mon 88701 and methods of use thereof |
EP2726618A1 (en) | 2011-06-30 | 2014-05-07 | Monsanto Technology LLC | Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof |
MX2014000533A (es) | 2011-07-13 | 2015-01-14 | Ms Technologies Llc | Evento 8264.42.32.1 de tolerancia apilada a los herbicidas, lineas de soya transgenicas relacionadas y su deteccion. |
WO2013012643A1 (en) | 2011-07-15 | 2013-01-24 | Syngenta Participations Ag | Polynucleotides encoding trehalose-6-phosphate phosphatase and methods of use thereof |
EP2809144B1 (en) | 2012-02-01 | 2019-11-13 | Dow AgroSciences LLC | Novel class of glyphosate resistance genes |
CA2908616A1 (en) * | 2013-04-05 | 2014-10-09 | Bayer Cropscience Nv | Brassica plants comprising mutant da1 alleles |
HUE042903T2 (hu) * | 2013-08-14 | 2019-07-29 | Inst Of Genetics And Developmental Biology | Eljárások mag- és szervméret modulálására növényekben |
GB201319876D0 (en) * | 2013-11-11 | 2013-12-25 | Plant Bioscience Ltd | Methods of modulating seed and organ size in plants |
EP3177644B1 (en) | 2014-08-05 | 2020-10-07 | Mabquest SA | Immunological reagents binding to pd-1 |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
GB2568182A (en) | 2016-08-03 | 2019-05-08 | Harvard College | Adenosine nucleobase editors and uses thereof |
-
2022
- 2022-08-29 US US17/822,822 patent/US20230074699A1/en active Pending
- 2022-08-29 CA CA3230167A patent/CA3230167A1/en active Pending
- 2022-08-29 WO PCT/US2022/075555 patent/WO2023034731A1/en active Application Filing
- 2022-08-30 AR ARP220102344A patent/AR126924A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
CA3230167A1 (en) | 2023-03-09 |
US20230074699A1 (en) | 2023-03-09 |
WO2023034731A1 (en) | 2023-03-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11802277B2 (en) | Thermostable Cas9 nucleases | |
KR102021585B1 (ko) | 두 개의 벡터로부터 발현된 Cas9 단백질을 이용한 유전자 발현 조절 방법 | |
EP3289081B1 (en) | Compositions and methods for the treatment of nucleotide repeat expansion disorders | |
Lampe et al. | A purified mariner transposase is sufficient to mediate transposition in vitro. | |
JP2022520428A (ja) | Ruvcドメインを有する酵素 | |
CN116096892A (zh) | 具有RuvC结构域的酶 | |
JPWO2013118878A1 (ja) | 環状rna及びタンパク質の製造方法 | |
KR102126641B1 (ko) | 재조합효소 돌연변이체 | |
AR126924A1 (es) | Modificación de genes de peptidasa de unión a ubiquitina en plantas para mejorar rasgos de rendimiento | |
JP2020508693A (ja) | C2c1エンドヌクレアーゼを含むゲノム編集用組成物およびこれを用いたゲノム編集方法 | |
AR126172A1 (es) | Modificación de factores de transcripción de la familia de factores reguladores del crecimiento en soja | |
AR124859A1 (es) | Métodos y composiciones para modificar los niveles de citoquinina oxidasa en plantas | |
CN113728097A (zh) | 具有ruvc结构域的酶 | |
Pospieszny et al. | Characterization of two distinct Polish isolates of Pepino mosaic virus | |
US11692166B2 (en) | Compositions and methods for improving alcohol tolerance in yeast | |
EP4165177A1 (en) | Enzymes with ruvc domains | |
AR126240A1 (es) | Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento | |
AR127300A1 (es) | Métodos para mejorar la fertilidad de la flor y el rendimiento de semillas | |
AR127236A1 (es) | Métodos para mejorar la fertilidad de la flor y el rendimiento de semillas | |
AR126362A1 (es) | Métodos y composiciones para mejorar el desarrollo del sistema radicular | |
CN116179513B (zh) | 一种Cpf1蛋白及其在基因编辑中的应用 | |
AR126938A1 (es) | Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento | |
CN104774860B (zh) | 适于转化细胞的构建体、系统及其应用 | |
US20230048564A1 (en) | Crispr-associated transposon systems and methods of using same | |
Mizia et al. | Development of a RAPD-based male-specific molecular marker in Japanese hop (Humulus japonicus Siebold and Zucc.) |