AR128687A1 - Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento - Google Patents

Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento

Info

Publication number
AR128687A1
AR128687A1 ARP230100528A ARP230100528A AR128687A1 AR 128687 A1 AR128687 A1 AR 128687A1 AR P230100528 A ARP230100528 A AR P230100528A AR P230100528 A ARP230100528 A AR P230100528A AR 128687 A1 AR128687 A1 AR 128687A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
bri1
guide nucleic
endogenous
gene
Prior art date
Application number
ARP230100528A
Other languages
English (en)
Inventor
Nicholas Bate
Lolita George Mathew
Devin Oconnor
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR128687A1 publication Critical patent/AR128687A1/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Esta invención se refiere a composiciones y métodos para modificar genes de Brasinoesteroide Insensible-1 (BRI1) en plantas, opcionalmente, para mejorar rasgos de rendimiento. La invención además se refiere a plantas que tienen mayores rasgos de rendimiento mejorados producidas usando los métodos y las composiciones de la invención. Reivindicación 1: Una planta o parte de planta de la misma que comprende por lo menos una mutación en un gen endógeno de Brasinoesteroide Insensible-1 (BRI1) que codifica un polipéptido BRII (polipéptido receptor de brasinoesteroides). Reivindicación 18: Una célula vegetal que comprende un sistema de edición, donde el sistema de edición comprende: (a) una proteína efectora CRISPR-Cas; y (b) un ácido nucleico guía que comprende una secuencia espaciadora con complementariedad a un gen diana endógeno que codifica un polipéptido BRII. Reivindicación 63: El ácido nucleico guía de la reivindicación 62, donde el ácido nucleico guía comprende un espaciador que comprende cualquiera de las secuencias de nucleótidos de SEQ ID Nº 96 - 100. Reivindicación 64: Un sistema que comprende el ácido nucleico guía de la reivindicación 62 o la reivindicación 63 y una proteína efectora CRISPR-Cas que se asocia con el ácido nucleico guía. Reivindicación 85: El gen BRI1 endógeno mutado de la reivindicación 84, donde el gen BRI1 endógeno mutado es un gen BRI1 endógeno mutado no natural.
ARP230100528A 2022-03-02 2023-03-02 Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento AR128687A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202263315646P 2022-03-02 2022-03-02

Publications (1)

Publication Number Publication Date
AR128687A1 true AR128687A1 (es) 2024-06-05

Family

ID=85726388

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP230100528A AR128687A1 (es) 2022-03-02 2023-03-02 Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento

Country Status (2)

Country Link
AR (1) AR128687A1 (es)
WO (1) WO2023168217A1 (es)

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