AR077115A1 - Produccion de isopreno usando las vias de mevalonato (mva) y la de no mevalonato (dxp) - Google Patents
Produccion de isopreno usando las vias de mevalonato (mva) y la de no mevalonato (dxp)Info
- Publication number
- AR077115A1 AR077115A1 ARP100102132A AR077115A1 AR 077115 A1 AR077115 A1 AR 077115A1 AR P100102132 A ARP100102132 A AR P100102132A AR 077115 A1 AR077115 A1 AR 077115A1
- Authority
- AR
- Argentina
- Prior art keywords
- polypeptide
- dxp
- mva
- mevalonate
- pathway
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se provee métodos para producir isopreno a partir de células cultivadas usando diversos componentes de la vía DXP y MVA, o componentes asociados con la vía DXP y la vía MVA, polipéptido redox que interactua con el cumulo de hierro y azufres e isopreno sintasa. También se provee composiciones que incluyen estas células cultivadas. Los métodos para produccion de isopreno son de utilidad en la industria de cubiertas y gomas. Reivindicacion 1: Células que comprenden (i) un ácido nucleico heterologo que codifica un polipéptido redox que interactua con el cumulo de hierro y azufre, un polipéptido de la vía DXP, un polipéptido de fa vía MVA y un polipéptido de isopreno sintasa o (ii) una copia duplicada de un ácido nucleico endogeno que codifica un polipéptido redox que interactua con el cumulo de hierro y azufre, un polipéptido de la vía DXP, un polipéptido de la vía MVA y un polipéptido de isopreno sintasa.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18793009P | 2009-06-17 | 2009-06-17 | |
US18794109P | 2009-06-17 | 2009-06-17 | |
US31497910P | 2010-03-17 | 2010-03-17 | |
US31498510P | 2010-03-17 | 2010-03-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR077115A1 true AR077115A1 (es) | 2011-08-03 |
Family
ID=43356749
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP100102132 AR077115A1 (es) | 2009-06-17 | 2010-06-16 | Produccion de isopreno usando las vias de mevalonato (mva) y la de no mevalonato (dxp) |
Country Status (10)
Country | Link |
---|---|
US (2) | US8507235B2 (es) |
EP (1) | EP2443244A4 (es) |
CN (1) | CN102753697A (es) |
AR (1) | AR077115A1 (es) |
BR (1) | BRPI1014123A2 (es) |
CA (1) | CA2765805A1 (es) |
MY (1) | MY158160A (es) |
SG (2) | SG10201403297UA (es) |
TW (2) | TW201412988A (es) |
WO (1) | WO2010148150A1 (es) |
Families Citing this family (56)
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SG192545A1 (en) | 2008-04-23 | 2013-08-30 | Danisco Us Inc | Isoprene synthase variants for improved microbial production of isoprene |
WO2010031077A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
WO2010031062A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using the archaeal lower mevalonate pathway |
WO2010031068A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
US8518686B2 (en) | 2009-04-23 | 2013-08-27 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用dxp及mva途徑之改良之異戊二烯製造 |
US20110039323A1 (en) * | 2009-08-14 | 2011-02-17 | Eric Lawrence Singsaas | Isoprene Production |
US8569562B2 (en) | 2009-12-18 | 2013-10-29 | Danisco Us Inc. | Purification of isoprene from renewable resources |
JP5855576B2 (ja) * | 2009-12-22 | 2016-02-09 | ダニスコ・ユーエス・インク | イソプレンガスの生成量を増大させるための膜バイオリアクター |
US8933282B2 (en) | 2010-06-17 | 2015-01-13 | Danisco Us Inc. | Fuel compositions comprising isoprene derivatives |
WO2012019169A1 (en) | 2010-08-06 | 2012-02-09 | Danisco Us Inc. | Production of isoprene under neutral ph conditions |
BR112013010551A8 (pt) | 2010-10-27 | 2018-07-03 | Danisco Us Inc | variantes de isopreno sintase para produção aprimorada de isopreno |
JP2014502844A (ja) | 2010-12-22 | 2014-02-06 | ダニスコ・ユーエス・インク | 組み換え細胞による五炭糖の生物学的生産 |
CN103476926A (zh) | 2010-12-22 | 2013-12-25 | 丹尼斯科美国公司 | 使用两种类型的ispg酶改善异戊二烯产量的组合物和方法 |
WO2012135591A2 (en) * | 2011-03-30 | 2012-10-04 | Amyris, Inc. | Microbial isoprenoid production using a heterologous dxp pathway |
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JP2014519811A (ja) | 2011-04-29 | 2014-08-21 | ダニスコ・ユーエス・インク | チオラーゼ、HMG−CoA合成酵素及びHMG−CoA還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産 |
JP2014528705A (ja) | 2011-08-04 | 2014-10-30 | ダニスコ・ユーエス・インク | アセトアセチルCoAシンターゼを用いるイソプレン、イソプレノイド前駆体、及びイソプレノイドの生産 |
US8951764B2 (en) | 2011-08-05 | 2015-02-10 | Danisco Us Inc. | Production of isoprenoids under neutral pH conditions |
PL222067B1 (pl) * | 2011-08-11 | 2016-06-30 | Inst Biotechnologii I Antybiotyków | Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu |
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JP6101275B2 (ja) | 2011-10-27 | 2017-03-22 | ダニスコ・ユーエス・インク | イソプレン産生時の溶解度が向上されたイソプレン合成酵素変異体 |
CA2859885A1 (en) | 2011-12-23 | 2013-06-27 | The Goodyear Tire & Rubber Company | Enhanced production of isoprene using host cells having decreased ispa activity |
WO2013096863A1 (en) * | 2011-12-23 | 2013-06-27 | The Regents Of The University Of California | Constructs and methods for improved isoprene biosynthesis |
EP2794876A1 (en) | 2011-12-23 | 2014-10-29 | Danisco US Inc. | Production of isoprene under reduced oxygen inlet levels |
US9315831B2 (en) | 2012-03-30 | 2016-04-19 | Danisco Us Inc. | Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids |
US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
US8895277B2 (en) | 2012-05-02 | 2014-11-25 | Danisco Us Inc. | Legume isoprene synthase for production of isoprene |
US20130323820A1 (en) * | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
US10030221B2 (en) | 2012-09-20 | 2018-07-24 | Danisco Us Inc. | Microtiter plates for controlled release of culture components to cell cultures |
WO2014066892A1 (en) * | 2012-10-26 | 2014-05-01 | Coelho Pedro S | De novo metabolic pathways for isoprene biosynthesis |
EP2935364A2 (en) | 2012-12-21 | 2015-10-28 | Danisco US Inc. | Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway |
BR112015021003A2 (pt) * | 2013-03-07 | 2017-07-18 | Calysta Inc | composições e métodos para a produção biológica de isopreno. |
US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
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CN103602626B (zh) * | 2013-10-12 | 2016-02-10 | 中国科学院青岛生物能源与过程研究所 | 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌 |
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US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
WO2016015021A1 (en) | 2014-07-24 | 2016-01-28 | The Regents Of The University Of California | Oxidative starch degradation by a new family of pmos |
CN106061870B (zh) | 2014-09-17 | 2018-10-02 | Wcco传送带公司 | 对环境友好的橡胶组合物 |
CN104372017B (zh) * | 2014-11-05 | 2017-06-30 | 中国科学院青岛生物能源与过程研究所 | 一种提高基因工程菌异戊二烯及其衍生物产量的方法及应用 |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
MX2019008882A (es) | 2017-01-26 | 2019-12-16 | Manus Bio Inc | Ingeniería metabólica para la producción microbiana de productos terpenoides. |
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CN110468091B (zh) * | 2018-05-09 | 2023-07-11 | 深圳艾格鑫科技有限公司 | 微生物及其用途 |
CN110468090B (zh) * | 2018-05-09 | 2023-07-04 | 深圳艾格鑫科技有限公司 | 微生物及其用途 |
CN108715825B (zh) * | 2018-06-01 | 2021-03-09 | 天津大学 | 基因过表达及获得的菌株、应用 |
US20200239796A1 (en) * | 2018-09-28 | 2020-07-30 | The Regents Of The University Of California | Host cells and methods for producing tricyclic sesquiterpenes, aviation and missile fuel precursors |
CN109609424B (zh) * | 2018-12-26 | 2020-07-31 | 浙江医药股份有限公司 | 用于生产法尼烯的大肠杆菌 |
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-
2010
- 2010-06-15 TW TW102141532A patent/TW201412988A/zh unknown
- 2010-06-15 TW TW99119621A patent/TWI427149B/zh not_active IP Right Cessation
- 2010-06-16 EP EP10790161.3A patent/EP2443244A4/en not_active Withdrawn
- 2010-06-16 MY MYPI2011006126A patent/MY158160A/en unknown
- 2010-06-16 US US12/817,134 patent/US8507235B2/en not_active Expired - Fee Related
- 2010-06-16 CA CA2765805A patent/CA2765805A1/en not_active Abandoned
- 2010-06-16 WO PCT/US2010/038904 patent/WO2010148150A1/en active Application Filing
- 2010-06-16 CN CN2010800359758A patent/CN102753697A/zh active Pending
- 2010-06-16 BR BRPI1014123A patent/BRPI1014123A2/pt not_active IP Right Cessation
- 2010-06-16 SG SG10201403297UA patent/SG10201403297UA/en unknown
- 2010-06-16 SG SG2011094562A patent/SG176936A1/en unknown
- 2010-06-16 AR ARP100102132 patent/AR077115A1/es unknown
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2013
- 2013-07-11 US US13/940,092 patent/US20140162337A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
TW201111512A (en) | 2011-04-01 |
SG10201403297UA (en) | 2014-10-30 |
US20140162337A1 (en) | 2014-06-12 |
EP2443244A4 (en) | 2014-11-26 |
EP2443244A1 (en) | 2012-04-25 |
CA2765805A1 (en) | 2010-12-23 |
WO2010148150A1 (en) | 2010-12-23 |
BRPI1014123A2 (pt) | 2019-07-02 |
US8507235B2 (en) | 2013-08-13 |
TW201412988A (zh) | 2014-04-01 |
MY158160A (en) | 2016-09-15 |
SG176936A1 (en) | 2012-02-28 |
TWI427149B (zh) | 2014-02-21 |
CN102753697A (zh) | 2012-10-24 |
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