AR030430A1 - Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d - Google Patents

Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d

Info

Publication number
AR030430A1
AR030430A1 ARP010103057A ARP010103057A AR030430A1 AR 030430 A1 AR030430 A1 AR 030430A1 AR P010103057 A ARP010103057 A AR P010103057A AR P010103057 A ARP010103057 A AR P010103057A AR 030430 A1 AR030430 A1 AR 030430A1
Authority
AR
Argentina
Prior art keywords
pathway
obtaining
organism
procedure
nucleinic
Prior art date
Application number
ARP010103057A
Other languages
English (en)
Inventor
R Badur
Michael Geiger
I Kunze
S Sommer
Original Assignee
Sungene Gmbh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2000130647 external-priority patent/DE10030647A1/de
Priority claimed from DE2000164454 external-priority patent/DE10064454A1/de
Application filed by Sungene Gmbh & Co Kgaa filed Critical Sungene Gmbh & Co Kgaa
Publication of AR030430A1 publication Critical patent/AR030430A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Procedimiento para la obtencion de químicos finos por cultivo de organismos, tales como plantas, que presentan una vía de shiquimato, modificada en comparacion con el tipo silvestre, por alteracion de la actividad de al menos una enzima de la vía de shiquimato se selecciona por lo menos una medida del grupo de las medidas A y B, teniendo A y B los significados siguientes: A: aumento de la activacion de por lo menos una enzima de la vía de shiquimato del tipo silvestre; B: incorporacion de por lo menos un gen en el organismo frente al que el tipo silvestre no presente ningun gen ortologico y que puentea la ruta metabolica de la vía de shiquimato. Preferentemente, en la medida A, se aumenta la actividad de por lo menos una enzima de la vía de shiquimato por sobreexpresion de ácidos nucleínicos, que codifican para proteínas con esta actividad enzimática. Más preferentemente, el ácido nucleico codifica una corismato-mutasa en el organismo. Composicion de ácido nucleico, que contiene un ácido nucleico codificante para un péptido de transicion plastídico y un ácido nucleico, que codifica para una proteína que contiene la secuencia e aminoácido SEQ ID No. 4 o una secuencia derivada de la misma por sustitucion, insercion o delecion de aminoácidos, que presenta una homología de por lo menos un 30% a nivel de aminoácido con la secuencia SEQ ID No. 4 y la propiedad enzimática de una corismato-mutasa. Organismo genéticamente modificado, siendo preciso que la modificacion genética cambia el flujo de metabolito de la vía de shiquimato, en comparacion con el tipo silvestre, y que el organismo presenta un contenido modificado en químicos finos, en comparacion con el tipo silvestre, tales como la vitamina E, vitamina K y/o ubiquinona. Procedimiento para la produccion de dichos organismos genéticamente modificados en donde se incorpora por lo menos un ácido nucleínico o por lo menos una composicion de ácido nucleínico segun lo descripto en el genoma del organismo de partida. Uso de los organismos genéticamente modificados para la produccion de químicos finos, o como alimento humano o animal, o para la obtencion de alimentos procesados.
ARP010103057A 2000-06-29 2001-06-27 Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d AR030430A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000130647 DE10030647A1 (de) 2000-06-29 2000-06-29 Veränderung des Gehalts an Feinchemikalien in Organismen durch genetische Veränderung des Shikimatweges
DE2000164454 DE10064454A1 (de) 2000-12-21 2000-12-21 Veränderung des Gehalts an Feinchemikalien in Organismen durch genetische Veränderung des Shikimatweges

Publications (1)

Publication Number Publication Date
AR030430A1 true AR030430A1 (es) 2003-08-20

Family

ID=26006169

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010103057A AR030430A1 (es) 2000-06-29 2001-06-27 Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d

Country Status (13)

Country Link
US (1) US7332649B2 (es)
EP (1) EP1294913B1 (es)
JP (1) JP2004501649A (es)
CN (1) CN1439054A (es)
AR (1) AR030430A1 (es)
AT (1) ATE389025T1 (es)
AU (1) AU2001266096A1 (es)
BR (1) BR0112052A (es)
CA (1) CA2413049A1 (es)
CZ (1) CZ20024144A3 (es)
DE (1) DE50113727D1 (es)
PL (1) PL360365A1 (es)
WO (1) WO2002000901A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0009763B8 (pt) 1999-04-15 2020-03-10 Calgene Llc sequência de ácido nucléico codificando uma preniltransferase, construção compreendendo a mesma, bem como métodos para alteração do teor de tocoferol, produção de um composto de tocoferol,e aumento do fluxo biossintético em uma célula
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
EP1356033B1 (en) 2000-08-07 2009-10-28 Monsanto Technology LLC Methyl-d-erythritol phosphate pathway genes
FR2817557B1 (fr) * 2000-12-05 2005-05-06 Aventis Cropscience Sa Nouvelles cibles pour herbicides et plantes transgeniques resistantes a ces herbicides
AU2007249135B2 (en) * 2000-12-05 2010-03-04 Bayer S.A.S. Novel targets for herbicides and transgenic plants resistant to said herbicides
EP1950305A1 (en) * 2001-05-09 2008-07-30 Monsanto Technology, LLC Tyr a genes and uses thereof
ATE419366T1 (de) * 2001-05-09 2009-01-15 Monsanto Technology Llc Tyra-gene und ihre verwendung
AU2002329759A1 (en) 2001-08-17 2003-03-03 Monsanto Technology Llc Methyltransferase genes and uses thereof
DE10212703A1 (de) * 2002-03-21 2003-10-02 Sungene Gmbh & Co Kgaa Erhöhung Vitamin-E-Gehalts in Organismen durch Erhöhung der 2-Methyl-6-phytythydrochinon-Methyltransferase-Aktivität
DE10231587A1 (de) * 2002-07-11 2004-01-29 Sungene Gmbh & Co. Kgaa Transgene Expressionskonstrukte und Verfahren zum Erhöhen des Vitamin E-Gehaltes in pflanzlichen Organismen
DE10232483A1 (de) * 2002-07-17 2004-02-05 Sungene Gmbh & Co. Kgaa Transgene Expressionskonstrukte und Verfahren zum Erhöhen des Vitamin E-Gehaltes in pflanzlichen Organismen
FR2844142B1 (fr) * 2002-09-11 2007-08-17 Bayer Cropscience Sa Plantes transformees a biosynthese de prenylquinones amelioree
US20110179523A1 (en) * 2008-09-30 2011-07-21 Basf Plant Science Gmbh Method for Producing a Transgenic Plant Cell, a Plant or a Part Thereof with Increased Resistance Biotic Stress
AU2010209874A1 (en) * 2009-01-28 2011-08-18 Basf Plant Science Company Gmbh Transgenic plants having altered nitrogen metabolism
CN113061619B (zh) * 2021-04-30 2022-06-24 中国烟草总公司郑州烟草研究院 与烟气苯酚释放量相关的突变基因TyrA

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
JPS63105688A (ja) * 1986-10-21 1988-05-10 Kyowa Hakko Kogyo Co Ltd Ｌ−フエニルアラニンの製造法
JP2578488B2 (ja) * 1988-03-04 1997-02-05 協和醗酵工業株式会社 アミノ酸の製造法
US5120837A (en) * 1989-09-20 1992-06-09 The Nutrasweet Company Dna encoding phe a feedback inhibition resistant enzyme analogues
US6087563A (en) 1996-01-29 2000-07-11 Arizona Board Of Regents On Behalf Of The University Of Arizona Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA
US6642434B1 (en) 1997-07-25 2003-11-04 University Of Community College System Of Nevada Transgenic plants with γ-tocopherol methyltransferase
DE19752647C1 (de) 1997-10-29 1999-06-24 Inst Pflanzengenetik & Kultur Reduktiion des Chlorophyllgehaltes in Ölpflanzensamen
AU757440B2 (en) * 1998-08-05 2003-02-20 Sungene Gmbh And Co. Kgaa DNA sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Also Published As

Publication number Publication date
US20060057687A1 (en) 2006-03-16
PL360365A1 (en) 2004-09-06
CN1439054A (zh) 2003-08-27
BR0112052A (pt) 2004-02-10
AU2001266096A1 (en) 2002-01-08
US7332649B2 (en) 2008-02-19
JP2004501649A (ja) 2004-01-22
ATE389025T1 (de) 2008-03-15
CZ20024144A3 (cs) 2003-05-14
DE50113727D1 (de) 2008-04-24
WO2002000901A1 (de) 2002-01-03
EP1294913A1 (de) 2003-03-26
EP1294913B1 (de) 2008-03-12
CA2413049A1 (en) 2002-01-03

Similar Documents

Publication Publication Date Title
AR030430A1 (es) Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d
ES2368221T3 (es) Producción recombinante de agentes antimicrobianos.
CN1816564B (zh) 能选择性杀伤癌细胞的RasGAP衍生肽
DE69738804D1 (de) Blütenorgan-spezifische promotersequenzen
EP1712625A4 (en) TRANSCRIPTION FACTOR ARABIDOPIS THALIANA, GENE ENCODING THE SAME AND USE THEREOF
WO2019004878A1 (ru) Препарат цистеиновой протеиназы пшеницы тритикаина-альфа, полученной в растворимой форме, и способ получения препарата
WO2012154024A1 (en) Cis-prenyl transferase from the plant hevea brasiliensis
CN1798838B (zh) 一种蛋白酶的抑制剂蛋白及其用途
CN104119445A (zh) 含有富含亮氨酸重复序列的融合蛋白及其制法和应用
AU2007208928A8 (en) Transgenic plant transformed with stress-responsive gene
CN107075501A (zh) 炎症报道系统
CN100390286C (zh) 新酶基因及其表达产物
JP5641477B2 (ja) Ｎｅｌｌ−１による組織・臓器異所再生制御技術
CN109628460A (zh) 一种超深渊来源抗菌肽hydramacin及其制备方法
ES2632431T3 (es) Moléculas de neurturina mejoradas
CN104119448A (zh) 含有富含亮氨酸重复序列的融合蛋白及其制法和应用
CN109517814A (zh) 有机磷降解酶的突变体及其应用
CN104119447A (zh) 含有富含亮氨酸重复序列的融合蛋白及其制法和应用
US20230287059A1 (en) A molecular strategy to protect against desiccation
Tao et al. Expression of apalbumin1 of Apis cerana cerana in the larvae of silkworm, Bombyx mori
CN107922463A (zh) 具有提高的光合能力的微生物
JPH05178896A (ja) 新規なタンパク質、それをコードする遺伝子及びその産生方法
CN101851627A (zh) 中华绒螯蟹半胱氨酸蛋白酶抑制剂EsCystatin基因编码蛋白及应用
KR101860902B1 (ko) 남극 요각류 티그리오푸스 킹세종엔시스 유래의 스테아로일-CoA 불포화효소 및 이의 용도
KR101687903B1 (ko) 멧누에 유래 특이적 Dpp 유전자 및 그 유전자의 발현

Legal Events

Date Code Title Description
FA Abandonment or withdrawal