AR029472A1 - Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante - Google Patents

Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante

Info

Publication number
AR029472A1
AR029472A1 ARP010100847A ARP010100847A AR029472A1 AR 029472 A1 AR029472 A1 AR 029472A1 AR P010100847 A ARP010100847 A AR P010100847A AR P010100847 A ARP010100847 A AR P010100847A AR 029472 A1 AR029472 A1 AR 029472A1
Authority
AR
Argentina
Prior art keywords
lignocellulosic biomass
fermenter
degree
thermotolering
yeast
Prior art date
Application number
ARP010100847A
Other languages
English (en)
Original Assignee
Ct Investig Energeticas Ciemat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Investig Energeticas Ciemat filed Critical Ct Investig Energeticas Ciemat
Publication of AR029472A1 publication Critical patent/AR029472A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16—Solid state fermenters, e.g. for koji production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Comprende las etapas de moler la biomasa lignocelulosica hasta un tamano de 15 a 30 mm, someter el producto obtenido a un pretratamiento mediante explosion a vapor a una temperatura de 190-230 degree C durante un intervalo de 1 a 10 minutos en un reactor, recoger el material pretratado en un ciclon y separar las fracciones líquida y solida mediante filtracion de un filtro de prensa, introducir la fraccion solida en un fermentador anadiendo una celulasa en una concentracion de 15 UPF por gramo de celulosa así como 12,6 Unidades Internacionales de enzima b-glucosidasa disueltas en tampon citrato pH 4,8 inocular el fermentador con un cultivo de la bacteria termotolerante Kluyveromyces marxianus CECT 10875, obtenida mediante mutagénesis química a partir de la cepa DER-26 de Kluyveromyces marxianus y mantener la mezcla en agitacion durante 72 horas a 42 degree C.
ARP010100847A 2000-02-24 2001-02-23 Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante AR029472A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200000439A ES2166316B1 (es) 2000-02-24 2000-02-24 Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante.

Publications (1)

Publication Number Publication Date
AR029472A1 true AR029472A1 (es) 2003-07-02

Family

ID=8492450

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010100847A AR029472A1 (es) 2000-02-24 2001-02-23 Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante

Country Status (8)

Country Link
US (2) US20020164730A1 (es)
EP (1) EP1130085B1 (es)
CN (1) CN1193099C (es)
AR (1) AR029472A1 (es)
AT (1) ATE305966T1 (es)
BR (1) BR0100762A (es)
DE (1) DE60113744T2 (es)
ES (1) ES2166316B1 (es)

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ES2166316A1 (es) 2002-04-01
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CN1340627A (zh) 2002-03-20
ATE305966T1 (de) 2005-10-15

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