AR018038A1 - Un procedimiento para producir un embrion animal, y los animales que se obtienen empleando este procedimiento - Google Patents
Un procedimiento para producir un embrion animal, y los animales que se obtienen empleando este procedimientoInfo
- Publication number
- AR018038A1 AR018038A1 ARP990100169A ARP990100169A AR018038A1 AR 018038 A1 AR018038 A1 AR 018038A1 AR P990100169 A ARP990100169 A AR P990100169A AR P990100169 A ARP990100169 A AR P990100169A AR 018038 A1 AR018038 A1 AR 018038A1
- Authority
- AR
- Argentina
- Prior art keywords
- cell
- animal
- nucleus
- procedure
- donor
- Prior art date
Links
- 241001465754 Metazoa Species 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 5
- 210000001161 mammalian embryo Anatomy 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 10
- 230000001605 fetal effect Effects 0.000 abstract 4
- 210000002950 fibroblast Anatomy 0.000 abstract 4
- 238000004519 manufacturing process Methods 0.000 abstract 4
- 238000010367 cloning Methods 0.000 abstract 2
- 210000000472 morula Anatomy 0.000 abstract 2
- 230000008672 reprogramming Effects 0.000 abstract 2
- 235000021120 animal protein Nutrition 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0273—Cloned vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Un procedimiento para producir un embrion animal que comprende las etapas siguientes: (a) obtener un fibroblasto fetal; (b) combinar el nucleodel fibroblasto fetal con una célula receptora adecuada sin fijar el fibroblasto fetal por manipulacion externa en la fase GO antes de la combinacion;(c) dejar que las células así obtenidas crezcan durante un período de tiempo para que se forme un blastocito, y, opcionalmente; (d) introducir la masa crecidade células en un animal madre. Los animales que seobtienen empleando el procecimiento descripto anteriormente. Con la tecnología del estado de latécnica se presentaba el problema de encontrar células donoras adecuadas para la transferencia nuclear con las cuales fuera posible producir un embrionanimal de la manera más conveniente y economica. Tal como es sabido, la mayor dificultad de la clonacion de embriones es la reprogramacion de nucleo de ADNde la célula donora correspondiente elegida. Así, a pesar de todos los resultados obtenidos en este campo, aun existían problemas respecto de unareprogramacion efectiva del ADN de nucleo donor que permitiera llevar la célula manipulada que contiene el nucleo nuevo a un estado cercano aldel de un cigota natural. Esto se manifestaba, entre otras cosas, como un rendimiento extremadamente bajo con respecto a la produccion deblastocitos embrionarios y una velocidad de division baja. Con el procedimiento mencionado más arriba se superan estas desventajas del estado de la tecnica,empleando fibroblastos fetales en calidad de célula donora para la transferencia nuclear. El nucleo de esta célula se combina con una célula receptora adecuaday la célula así obtenida se hace crecer durante un período de tiempo, de manera de formar una morula. La morula así obtenida puede opcionalmente volver a serusada para la clonacion y ser introducida dentro de un animal madre para el alumbramiento. Empleando el procedimiento indicado mas arriba se puede lograr unincremento de la produccion de proteínas animales homologas, una modificacion en los productos animales tales como la leche misma, o la produccion de
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP1998/000230 WO1999036510A1 (de) | 1998-01-16 | 1998-01-16 | Effizienter kerntransfer mit fetalen fibroblasten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR018038A1 true AR018038A1 (es) | 2001-10-31 |
Family
ID=8166837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990100169A AR018038A1 (es) | 1998-01-16 | 1999-01-18 | Un procedimiento para producir un embrion animal, y los animales que se obtienen empleando este procedimiento |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6600087B1 (es) |
| EP (1) | EP0972017B1 (es) |
| JP (1) | JP4102450B2 (es) |
| AR (1) | AR018038A1 (es) |
| AT (1) | ATE278775T1 (es) |
| AU (1) | AU739902B2 (es) |
| CA (1) | CA2259368C (es) |
| DE (1) | DE59812083D1 (es) |
| RU (1) | RU2216592C2 (es) |
| WO (1) | WO1999036510A1 (es) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7304204B2 (en) | 1995-08-31 | 2007-12-04 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
| GB9517779D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| AU8154398A (en) * | 1997-01-10 | 1999-01-25 | University Of Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
| US7361804B1 (en) | 1997-02-19 | 2008-04-22 | Roslin Institute (Edinburgh) | Unactivated oocytes in nuclear transfer to produce ungulates |
| WO2002009507A1 (de) * | 2000-07-27 | 2002-02-07 | Apogene Gmbh & Co. Kg | Somatischer klonierungs-gentransfer zur produktion von rekombinanten proteinen, zellen und organen |
| EP1687421B1 (en) * | 2003-09-30 | 2018-06-27 | Sterrenbeld Biotechnologie North America, Inc. | A process for producing exogenous protein in the milk of transgenic mammals and a process for purifying proteins therefrom |
| SG174053A1 (en) | 2006-09-01 | 2011-09-29 | Therapeutic Human Polyclonals Inc | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals |
| US8912386B2 (en) | 2007-03-28 | 2014-12-16 | University Of Iowa Research Foundation | Transgenic pig model of cystic fibrosis |
| AU2008233256B2 (en) | 2007-03-28 | 2013-06-27 | The Curators Of The University Of Missouri | Transgenic animal models of disease |
| US8618352B2 (en) | 2007-03-28 | 2013-12-31 | University Of Iowa Research Foundation | Transgenic porcine models of cystic fibrosis |
| CN101715485A (zh) * | 2007-04-06 | 2010-05-26 | 国际干细胞公司 | 源于人单性生殖胚泡的患者特异性干细胞系 |
| US9339270B2 (en) | 2010-10-11 | 2016-05-17 | Cook Medical Technologies Llc | Medical devices with detachable pivotable jaws |
| US8545519B2 (en) | 2009-12-22 | 2013-10-01 | Cook Medical Technologies Llc | Medical devices with detachable pivotable jaws |
| US10010336B2 (en) | 2009-12-22 | 2018-07-03 | Cook Medical Technologies, Inc. | Medical devices with detachable pivotable jaws |
| DK2515770T3 (en) | 2009-12-22 | 2019-02-25 | Cook Medical Technologies Llc | MEDICAL DEVICES WITH REMOVABLE THREADABLE BUYERS |
| ES2738476T3 (es) | 2010-08-11 | 2020-01-23 | Inst Nat Sante Rech Med | Cocultivos de células del cúmulo y embriones durante procedimientos de fertilización in vitro |
| BR112013008755B1 (pt) | 2010-10-11 | 2021-04-27 | Cook Medical Technologies Llc | Dispositivo médico e método para operar um dispositivo médico |
| EP2627264B1 (en) | 2010-10-11 | 2015-06-17 | Cook Medical Technologies LLC | Medical devices with detachable pivotable jaws |
| EP2651316B1 (en) | 2010-12-15 | 2016-07-06 | Cook Medical Technologies LLC | Medical devices with detachable pivotable jaws |
| RU2495932C1 (ru) * | 2012-05-31 | 2013-10-20 | Федеральное государственное бюджетное учреждение науки Институт химической физики им. Н.Н. Семенова Российской академии наук (ИХФ РАН) | Способ лазерного слияния бластомеров внутри ранних доимплантационных эмбрионов млекопитающих без нарушения их целостности |
| CN103999820A (zh) * | 2014-05-12 | 2014-08-27 | 西南科技大学 | 一种杂交黄牛育肥方法 |
| CN105981686A (zh) * | 2015-02-27 | 2016-10-05 | 徐小芹 | 一种兔子的饲养方法 |
| CN107691363A (zh) * | 2017-11-01 | 2018-02-16 | 黄业钧 | 一种哺乳犊牛饲养与管理方法 |
| CN111296361A (zh) * | 2020-04-02 | 2020-06-19 | 固原富民农业科技发展有限公司 | 黄牛的科学繁育及生态养殖方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9517780D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US6215041B1 (en) * | 1997-01-10 | 2001-04-10 | University Of Mmassachusetts | Cloning using donor nuclei from a non-quiesecent somatic cells |
| AU8154398A (en) * | 1997-01-10 | 1999-01-25 | University Of Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| GB9703550D0 (en) | 1997-02-20 | 1997-04-09 | Ppl Therapeutics Scotland Ltd | Cell mediated transgenesis |
| US6011197A (en) * | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
-
1998
- 1998-01-16 AU AU66144/98A patent/AU739902B2/en not_active Ceased
- 1998-01-16 WO PCT/EP1998/000230 patent/WO1999036510A1/de active IP Right Grant
- 1998-01-16 EP EP98907950A patent/EP0972017B1/de not_active Expired - Lifetime
- 1998-01-16 RU RU2000122047/13A patent/RU2216592C2/ru not_active IP Right Cessation
- 1998-01-16 JP JP50362699A patent/JP4102450B2/ja not_active Expired - Fee Related
- 1998-01-16 AT AT98907950T patent/ATE278775T1/de not_active IP Right Cessation
- 1998-01-16 CA CA2259368A patent/CA2259368C/en not_active Expired - Fee Related
- 1998-01-16 US US09/230,625 patent/US6600087B1/en not_active Expired - Fee Related
- 1998-01-16 DE DE59812083T patent/DE59812083D1/de not_active Expired - Lifetime
-
1999
- 1999-01-18 AR ARP990100169A patent/AR018038A1/es active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ATE278775T1 (de) | 2004-10-15 |
| US6600087B1 (en) | 2003-07-29 |
| AU739902B2 (en) | 2001-10-25 |
| JP2000512159A (ja) | 2000-09-19 |
| DE59812083D1 (de) | 2004-11-11 |
| AU6614498A (en) | 1999-08-02 |
| CA2259368C (en) | 2010-10-05 |
| RU2216592C2 (ru) | 2003-11-20 |
| JP4102450B2 (ja) | 2008-06-18 |
| EP0972017A1 (de) | 2000-01-19 |
| WO1999036510A1 (de) | 1999-07-22 |
| CA2259368A1 (en) | 1999-07-16 |
| EP0972017B1 (de) | 2004-10-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |