ZA202205093B - Biochemical reaction methods and reagents comprising intrinsically disordered regions - Google Patents

Biochemical reaction methods and reagents comprising intrinsically disordered regions

Info

Publication number
ZA202205093B
ZA202205093B ZA2022/05093A ZA202205093A ZA202205093B ZA 202205093 B ZA202205093 B ZA 202205093B ZA 2022/05093 A ZA2022/05093 A ZA 2022/05093A ZA 202205093 A ZA202205093 A ZA 202205093A ZA 202205093 B ZA202205093 B ZA 202205093B
Authority
ZA
South Africa
Prior art keywords
macromolecules
polypeptides
biochemical reaction
relates
idrs
Prior art date
Application number
ZA2022/05093A
Other languages
English (en)
Inventor
Niall Armes
Hannah Williams
Matthew Forrest
Mathew Parker
Sidong Liu
Lauren Parker
Original Assignee
Biocrucible Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocrucible Ltd filed Critical Biocrucible Ltd
Publication of ZA202205093B publication Critical patent/ZA202205093B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/507Recombinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10Nucleic acid binding proteins
    • C12Q2522/101Single or double stranded nucleic acid binding proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ZA2022/05093A 2019-11-11 2022-05-09 Biochemical reaction methods and reagents comprising intrinsically disordered regions ZA202205093B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Publications (1)

Publication Number Publication Date
ZA202205093B true ZA202205093B (en) 2024-10-30

Family

ID=69062276

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2022/05093A ZA202205093B (en) 2019-11-11 2022-05-09 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Country Status (13)

Country Link
US (1) US11807899B2 (enExample)
EP (1) EP4058599A1 (enExample)
JP (2) JP7840266B2 (enExample)
KR (1) KR20220113948A (enExample)
CN (1) CN114945682A (enExample)
AU (1) AU2020382121A1 (enExample)
BR (1) BR112022009072A2 (enExample)
CA (1) CA3157435A1 (enExample)
GB (1) GB201916379D0 (enExample)
IL (1) IL292885A (enExample)
MX (1) MX2022005677A (enExample)
WO (1) WO2021094746A1 (enExample)
ZA (1) ZA202205093B (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
CN116251621B (zh) * 2022-12-13 2024-08-23 中国科学院基础医学与肿瘤研究所(筹) 一种提高氯化血红素的过氧化物酶活性的方法
WO2024178628A1 (en) * 2023-02-28 2024-09-06 Sun Yat-Sen University Epigenetic editor assisted by phase separation proteins
JP2024179837A (ja) * 2023-06-16 2024-12-26 国立大学法人 東京大学 酵素の検出方法及び酵素の検出キット
KR20250022285A (ko) 2023-08-07 2025-02-17 포항공과대학교 산학협력단 액체-액체 상분리를 통한 scotin 프롤린-풍부 도메인 생체분자 응축물 및 그의 응용
GB2643378A (en) * 2024-07-15 2026-02-18 Biocrucible Ltd Reagents and methods

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005103670A1 (ja) 2004-04-21 2005-11-03 Toray Industries, Inc. ラボオンチップ用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
WO2008108927A2 (en) * 2007-03-06 2008-09-12 Regenerx Biopharmaceuticals, Inc. Methods and compositions for stabilizing polypeptides
CN101743063B (zh) 2007-05-03 2012-12-12 科隆迪亚戈有限公司 分析方法
US8560130B2 (en) 2007-12-04 2013-10-15 Lester F. Ludwig Software controlled lab-on-a-chip emulation
CN108088824B (zh) 2008-03-14 2021-06-04 雅培快速诊断耶拿有限公司 一种用于检测分析物的系统
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
SG179200A1 (en) * 2009-09-16 2012-04-27 Univ Massey Fusion polypeptides and uses thereof
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
US10533167B2 (en) * 2016-06-09 2020-01-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
WO2018100408A1 (en) * 2016-11-30 2018-06-07 Sasinapas Co.,Ltd. Modified peptides
US20210047659A1 (en) * 2018-01-24 2021-02-18 The Trustees Of Princeton University System and method for inducing clusters of gene regulatory proteins targeted to specific genomic loci

Also Published As

Publication number Publication date
CN114945682A (zh) 2022-08-26
AU2020382121A8 (en) 2022-07-28
GB201916379D0 (en) 2019-12-25
US11807899B2 (en) 2023-11-07
BR112022009072A2 (pt) 2022-07-26
US20220112547A1 (en) 2022-04-14
CA3157435A1 (en) 2021-05-20
WO2021094746A1 (en) 2021-05-20
AU2020382121A1 (en) 2022-06-16
JP2023501002A (ja) 2023-01-17
JP2026041819A (ja) 2026-03-10
JP7840266B2 (ja) 2026-04-03
MX2022005677A (es) 2022-09-07
US20220145378A1 (en) 2022-05-12
EP4058599A1 (en) 2022-09-21
IL292885A (en) 2022-07-01
KR20220113948A (ko) 2022-08-17

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