CN114945682A - 包含固有无序区域的生化反应方法和试剂 - Google Patents

包含固有无序区域的生化反应方法和试剂 Download PDF

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Publication number
CN114945682A
CN114945682A CN202080092644.1A CN202080092644A CN114945682A CN 114945682 A CN114945682 A CN 114945682A CN 202080092644 A CN202080092644 A CN 202080092644A CN 114945682 A CN114945682 A CN 114945682A
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idr
polypeptide
macromolecule
reaction
amino acid
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Chinese (zh)
Inventor
N·阿姆斯
H·威廉姆斯
M·佛利斯特
M·帕克
刘思东
L·帕克
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Bio Crucible Ltd
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Bio Crucible Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080092644.1A 2019-11-11 2020-11-11 包含固有无序区域的生化反应方法和试剂 Pending CN114945682A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
GB1916379.9 2019-11-11
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Publications (1)

Publication Number Publication Date
CN114945682A true CN114945682A (zh) 2022-08-26

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US (1) US11807899B2 (enExample)
EP (1) EP4058599A1 (enExample)
JP (2) JP7840266B2 (enExample)
KR (1) KR20220113948A (enExample)
CN (1) CN114945682A (enExample)
AU (1) AU2020382121A1 (enExample)
BR (1) BR112022009072A2 (enExample)
CA (1) CA3157435A1 (enExample)
GB (1) GB201916379D0 (enExample)
IL (1) IL292885A (enExample)
MX (1) MX2022005677A (enExample)
WO (1) WO2021094746A1 (enExample)
ZA (1) ZA202205093B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
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CN116251621A (zh) * 2022-12-13 2023-06-13 中国科学院基础医学与肿瘤研究所(筹) 一种提高氯化血红素的过氧化物酶活性的方法

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CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
WO2024178628A1 (en) * 2023-02-28 2024-09-06 Sun Yat-Sen University Epigenetic editor assisted by phase separation proteins
JP2024179837A (ja) * 2023-06-16 2024-12-26 国立大学法人 東京大学 酵素の検出方法及び酵素の検出キット
KR20250022285A (ko) 2023-08-07 2025-02-17 포항공과대학교 산학협력단 액체-액체 상분리를 통한 scotin 프롤린-풍부 도메인 생체분자 응축물 및 그의 응용
GB2643378A (en) * 2024-07-15 2026-02-18 Biocrucible Ltd Reagents and methods

Citations (3)

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US20050112631A1 (en) * 2002-02-21 2005-05-26 Olaf Piepenburg Recombinase polymerase amplification
WO2008108927A2 (en) * 2007-03-06 2008-09-12 Regenerx Biopharmaceuticals, Inc. Methods and compositions for stabilizing polypeptides
CN110036105A (zh) * 2016-11-30 2019-07-19 萨西那帕斯有限公司 经修饰的肽

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US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005103670A1 (ja) 2004-04-21 2005-11-03 Toray Industries, Inc. ラボオンチップ用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
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SG179200A1 (en) * 2009-09-16 2012-04-27 Univ Massey Fusion polypeptides and uses thereof
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
US10533167B2 (en) * 2016-06-09 2020-01-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
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Patent Citations (3)

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US20050112631A1 (en) * 2002-02-21 2005-05-26 Olaf Piepenburg Recombinase polymerase amplification
WO2008108927A2 (en) * 2007-03-06 2008-09-12 Regenerx Biopharmaceuticals, Inc. Methods and compositions for stabilizing polypeptides
CN110036105A (zh) * 2016-11-30 2019-07-19 萨西那帕斯有限公司 经修饰的肽

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116251621A (zh) * 2022-12-13 2023-06-13 中国科学院基础医学与肿瘤研究所(筹) 一种提高氯化血红素的过氧化物酶活性的方法

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AU2020382121A8 (en) 2022-07-28
GB201916379D0 (en) 2019-12-25
US11807899B2 (en) 2023-11-07
BR112022009072A2 (pt) 2022-07-26
US20220112547A1 (en) 2022-04-14
CA3157435A1 (en) 2021-05-20
WO2021094746A1 (en) 2021-05-20
AU2020382121A1 (en) 2022-06-16
JP2023501002A (ja) 2023-01-17
JP2026041819A (ja) 2026-03-10
JP7840266B2 (ja) 2026-04-03
MX2022005677A (es) 2022-09-07
ZA202205093B (en) 2024-10-30
US20220145378A1 (en) 2022-05-12
EP4058599A1 (en) 2022-09-21
IL292885A (en) 2022-07-01
KR20220113948A (ko) 2022-08-17

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