JP7840266B2 - 生化学反応方法及び天然変性領域を含む試薬 - Google Patents

生化学反応方法及び天然変性領域を含む試薬

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Publication number
JP7840266B2
JP7840266B2 JP2022553222A JP2022553222A JP7840266B2 JP 7840266 B2 JP7840266 B2 JP 7840266B2 JP 2022553222 A JP2022553222 A JP 2022553222A JP 2022553222 A JP2022553222 A JP 2022553222A JP 7840266 B2 JP7840266 B2 JP 7840266B2
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idr
reaction
polypeptide
amino acid
rpa
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Japanese (ja)
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JP2023501002A (ja
JP2023501002A5 (enExample
Inventor
アームズ、ナイオール
ウィリアムズ、ハンナ
フォレスト、マシュー
パーカー、マシュー
リュー、シドン
パーカー、ローレン
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バイオクルーシブル リミテッド
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Priority to JP2025207502A priority Critical patent/JP2026041819A/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022553222A 2019-11-11 2020-11-11 生化学反応方法及び天然変性領域を含む試薬 Active JP7840266B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025207502A JP2026041819A (ja) 2019-11-11 2025-11-28 生化学反応方法及び天然変性領域を含む試薬

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
GB1916379.9 2019-11-11
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Related Child Applications (1)

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JP2025207502A Division JP2026041819A (ja) 2019-11-11 2025-11-28 生化学反応方法及び天然変性領域を含む試薬

Publications (3)

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JP2023501002A JP2023501002A (ja) 2023-01-17
JP2023501002A5 JP2023501002A5 (enExample) 2023-11-20
JP7840266B2 true JP7840266B2 (ja) 2026-04-03

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JP2025207502A Pending JP2026041819A (ja) 2019-11-11 2025-11-28 生化学反応方法及び天然変性領域を含む試薬

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Country Status (13)

Country Link
US (1) US11807899B2 (enExample)
EP (1) EP4058599A1 (enExample)
JP (2) JP7840266B2 (enExample)
KR (1) KR20220113948A (enExample)
CN (1) CN114945682A (enExample)
AU (1) AU2020382121A1 (enExample)
BR (1) BR112022009072A2 (enExample)
CA (1) CA3157435A1 (enExample)
GB (1) GB201916379D0 (enExample)
IL (1) IL292885A (enExample)
MX (1) MX2022005677A (enExample)
WO (1) WO2021094746A1 (enExample)
ZA (1) ZA202205093B (enExample)

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Publication number Priority date Publication date Assignee Title
CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
CN116251621B (zh) * 2022-12-13 2024-08-23 中国科学院基础医学与肿瘤研究所(筹) 一种提高氯化血红素的过氧化物酶活性的方法
WO2024178628A1 (en) * 2023-02-28 2024-09-06 Sun Yat-Sen University Epigenetic editor assisted by phase separation proteins
JP2024179837A (ja) * 2023-06-16 2024-12-26 国立大学法人 東京大学 酵素の検出方法及び酵素の検出キット
KR20250022285A (ko) 2023-08-07 2025-02-17 포항공과대학교 산학협력단 액체-액체 상분리를 통한 scotin 프롤린-풍부 도메인 생체분자 응축물 및 그의 응용
GB2643378A (en) * 2024-07-15 2026-02-18 Biocrucible Ltd Reagents and methods

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JP2009520461A (ja) 2005-09-09 2009-05-28 ライフ テクノロジーズ コーポレーション Ｓｓｂ−ポリメラーゼ融合タンパク質
JP2013505016A (ja) 2009-09-16 2013-02-14 マッシー ユニヴァーシティー 融合ポリペプチドおよびその使用
JP2015512651A (ja) 2012-04-12 2015-04-30 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
US20170355977A1 (en) 2016-06-09 2017-12-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
WO2019147611A2 (en) 2018-01-24 2019-08-01 The Trustees Of Princeton University System and method for inducing clusters of gene regulatory proteins targeted to specific genomic loci

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US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005103670A1 (ja) 2004-04-21 2005-11-03 Toray Industries, Inc. ラボオンチップ用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
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WO2008108927A2 (en) * 2007-03-06 2008-09-12 Regenerx Biopharmaceuticals, Inc. Methods and compositions for stabilizing polypeptides
CN101743063B (zh) 2007-05-03 2012-12-12 科隆迪亚戈有限公司 分析方法
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JP2009520461A (ja) 2005-09-09 2009-05-28 ライフ テクノロジーズ コーポレーション Ｓｓｂ−ポリメラーゼ融合タンパク質
JP2013505016A (ja) 2009-09-16 2013-02-14 マッシー ユニヴァーシティー 融合ポリペプチドおよびその使用
JP2015512651A (ja) 2012-04-12 2015-04-30 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
US20170355977A1 (en) 2016-06-09 2017-12-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
WO2019147611A2 (en) 2018-01-24 2019-08-01 The Trustees Of Princeton University System and method for inducing clusters of gene regulatory proteins targeted to specific genomic loci

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Also Published As

Publication number Publication date
CN114945682A (zh) 2022-08-26
AU2020382121A8 (en) 2022-07-28
GB201916379D0 (en) 2019-12-25
US11807899B2 (en) 2023-11-07
BR112022009072A2 (pt) 2022-07-26
US20220112547A1 (en) 2022-04-14
CA3157435A1 (en) 2021-05-20
WO2021094746A1 (en) 2021-05-20
AU2020382121A1 (en) 2022-06-16
JP2023501002A (ja) 2023-01-17
JP2026041819A (ja) 2026-03-10
MX2022005677A (es) 2022-09-07
ZA202205093B (en) 2024-10-30
US20220145378A1 (en) 2022-05-12
EP4058599A1 (en) 2022-09-21
IL292885A (en) 2022-07-01
KR20220113948A (ko) 2022-08-17

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