AU2020382121B2 - Biochemical reaction methods and reagents comprising intrinsically disordered regions - Google Patents
Biochemical reaction methods and reagents comprising intrinsically disordered regionsInfo
- Publication number
- AU2020382121B2 AU2020382121B2 AU2020382121A AU2020382121A AU2020382121B2 AU 2020382121 B2 AU2020382121 B2 AU 2020382121B2 AU 2020382121 A AU2020382121 A AU 2020382121A AU 2020382121 A AU2020382121 A AU 2020382121A AU 2020382121 B2 AU2020382121 B2 AU 2020382121B2
- Authority
- AU
- Australia
- Prior art keywords
- idr
- polypeptide
- reaction
- polymerase
- functional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
- C12Q2522/10—Nucleic acid binding proteins
- C12Q2522/101—Single or double stranded nucleic acid binding proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1916379.9 | 2019-11-11 | ||
| GBGB1916379.9A GB201916379D0 (en) | 2019-11-11 | 2019-11-11 | Biochemical reaction methods and reagents |
| PCT/GB2020/052866 WO2021094746A1 (en) | 2019-11-11 | 2020-11-11 | Biochemical reaction methods and reagents comprising intrinsically disordered regions |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU2020382121A1 AU2020382121A1 (en) | 2022-06-16 |
| AU2020382121A8 AU2020382121A8 (en) | 2022-07-28 |
| AU2020382121B2 true AU2020382121B2 (en) | 2026-06-18 |
Family
ID=69062276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020382121A Active AU2020382121B2 (en) | 2019-11-11 | 2020-11-11 | Biochemical reaction methods and reagents comprising intrinsically disordered regions |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US12618102B2 (enExample) |
| EP (1) | EP4058599A1 (enExample) |
| JP (2) | JP7840266B2 (enExample) |
| KR (1) | KR20220113948A (enExample) |
| CN (1) | CN114945682A (enExample) |
| AU (1) | AU2020382121B2 (enExample) |
| BR (1) | BR112022009072A2 (enExample) |
| CA (1) | CA3157435A1 (enExample) |
| GB (1) | GB201916379D0 (enExample) |
| IL (1) | IL292885A (enExample) |
| MX (1) | MX2022005677A (enExample) |
| WO (1) | WO2021094746A1 (enExample) |
| ZA (1) | ZA202205093B (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998406B (zh) * | 2018-08-03 | 2022-05-10 | 福州大学 | 一种人类原代培养细胞基因组编辑、定点基因敲入方法 |
| GB201916379D0 (en) | 2019-11-11 | 2019-12-25 | Biocrucible Ltd | Biochemical reaction methods and reagents |
| CN113355389B (zh) * | 2020-03-05 | 2022-11-15 | 广西扬翔股份有限公司 | 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用 |
| EP3992635A1 (en) | 2020-10-29 | 2022-05-04 | midge medical GmbH | System and a device for detecting a target analyte |
| US20240110221A1 (en) * | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Methods of modulating clustering kinetics |
| CN116251621B (zh) * | 2022-12-13 | 2024-08-23 | 中国科学院基础医学与肿瘤研究所(筹) | 一种提高氯化血红素的过氧化物酶活性的方法 |
| WO2024178628A1 (en) * | 2023-02-28 | 2024-09-06 | Sun Yat-Sen University | Epigenetic editor assisted by phase separation proteins |
| US12612667B1 (en) * | 2023-05-01 | 2026-04-28 | National Technology & Engineering Solutions Of Sandia, Llc | Solid phase recombinase polymerase amplification for human pathogen identification |
| JP2024179837A (ja) * | 2023-06-16 | 2024-12-26 | 国立大学法人 東京大学 | 酵素の検出方法及び酵素の検出キット |
| KR20250022285A (ko) | 2023-08-07 | 2025-02-17 | 포항공과대학교 산학협력단 | 액체-액체 상분리를 통한 scotin 프롤린-풍부 도메인 생체분자 응축물 및 그의 응용 |
| WO2025137528A1 (en) * | 2023-12-20 | 2025-06-26 | Abbott Laboratories | Reducing agent compositions for isothermal amplification of nucleic acids |
| GB2643378A (en) * | 2024-07-15 | 2026-02-18 | Biocrucible Ltd | Reagents and methods |
| WO2026131829A1 (en) | 2024-12-16 | 2026-06-25 | Neoenta LLC | Devices for analysis of a biological sample and manufacturing methods thereof, and methods of anaylsis of a biological sample |
| GB202500508D0 (en) | 2025-01-15 | 2025-02-26 | Biocrucible Ltd | Molecular diagnostic device |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050112631A1 (en) * | 2002-02-21 | 2005-05-26 | Olaf Piepenburg | Recombinase polymerase amplification |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1499738T3 (da) | 2002-02-21 | 2008-11-17 | Asm Scient Inc | Recombinase-polymerease-amplifikation |
| CA2560758A1 (en) | 2004-04-21 | 2005-11-03 | Toray Industries, Inc. | Lab-on-chip substrate |
| DE102005052752A1 (de) | 2005-11-04 | 2007-05-10 | Clondiag Chip Technologies Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| EP2829615B1 (en) | 2005-07-25 | 2018-05-09 | Alere San Diego, Inc. | Kit for multiplexing recombinase polymerase amplification |
| US20070059713A1 (en) | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
| EP2029782B1 (en) | 2006-05-04 | 2014-11-26 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| WO2008108927A2 (en) * | 2007-03-06 | 2008-09-12 | Regenerx Biopharmaceuticals, Inc. | Methods and compositions for stabilizing polypeptides |
| CA2946024C (en) | 2007-05-03 | 2020-06-02 | Clondiag Gmbh | Devices and methods for detecting analytes |
| US8560130B2 (en) | 2007-12-04 | 2013-10-15 | Lester F. Ludwig | Software controlled lab-on-a-chip emulation |
| CN102026725B (zh) | 2008-03-14 | 2014-10-29 | 科隆迪亚戈有限公司 | 一种用于检测样品中分析物的装置及其方法 |
| WO2010141940A1 (en) | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| KR20120093882A (ko) | 2009-09-16 | 2012-08-23 | 마쎄이 유니버시티 | 융합 폴리펩티드 및 이들의 용도 |
| JP6490576B2 (ja) | 2012-04-12 | 2019-03-27 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | 重合反応のための合成核酸 |
| US10533167B2 (en) | 2016-06-09 | 2020-01-14 | The Trustees Of Princeton University | Optogenetic tool for rapid and reversible clustering of proteins |
| WO2018100408A1 (en) * | 2016-11-30 | 2018-06-07 | Sasinapas Co.,Ltd. | Modified peptides |
| EP3743435B1 (en) | 2018-01-24 | 2023-08-09 | The Trustees Of Princeton University | System and method for inducing clusters of gene regulatory proteins targeted to specific genomic loci |
| GB201916379D0 (en) | 2019-11-11 | 2019-12-25 | Biocrucible Ltd | Biochemical reaction methods and reagents |
-
2019
- 2019-11-11 GB GBGB1916379.9A patent/GB201916379D0/en not_active Ceased
-
2020
- 2020-11-11 WO PCT/GB2020/052866 patent/WO2021094746A1/en not_active Ceased
- 2020-11-11 CN CN202080092644.1A patent/CN114945682A/zh active Pending
- 2020-11-11 BR BR112022009072A patent/BR112022009072A2/pt not_active IP Right Cessation
- 2020-11-11 MX MX2022005677A patent/MX2022005677A/es unknown
- 2020-11-11 AU AU2020382121A patent/AU2020382121B2/en active Active
- 2020-11-11 IL IL292885A patent/IL292885A/en unknown
- 2020-11-11 JP JP2022553222A patent/JP7840266B2/ja active Active
- 2020-11-11 CA CA3157435A patent/CA3157435A1/en active Pending
- 2020-11-11 KR KR1020227019839A patent/KR20220113948A/ko active Pending
- 2020-11-11 EP EP20817468.0A patent/EP4058599A1/en active Pending
-
2021
- 2021-11-17 US US17/528,967 patent/US12618102B2/en active Active
- 2021-11-19 US US17/531,349 patent/US11807899B2/en active Active
-
2022
- 2022-05-09 ZA ZA2022/05093A patent/ZA202205093B/en unknown
-
2025
- 2025-11-28 JP JP2025207502A patent/JP2026041819A/ja active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050112631A1 (en) * | 2002-02-21 | 2005-05-26 | Olaf Piepenburg | Recombinase polymerase amplification |
Non-Patent Citations (1)
| Title |
|---|
| HUI YIN TAN, LUKE A. WILCZEK, SASHEEN POTTINGER, MARIA MANOSAS, CONG YU, TRONG NGUYENDUC, PIERO R. BIANCO: "The intrinsically disordered linker of E . coli SSB is critical for the release from single-stranded DNA : SSB IDL is Required for DNA Release", PROTEIN SCIENCE, WILEY, US, vol. 26, no. 4, 1 April 2017 (2017-04-01), US, pages 700 - 717, XP055770349, ISSN: 0961-8368, DOI: 10.1002/pro.3115 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201916379D0 (en) | 2019-12-25 |
| AU2020382121A1 (en) | 2022-06-16 |
| IL292885A (en) | 2022-07-01 |
| JP7840266B2 (ja) | 2026-04-03 |
| CA3157435A1 (en) | 2021-05-20 |
| MX2022005677A (es) | 2022-09-07 |
| CN114945682A (zh) | 2022-08-26 |
| JP2023501002A (ja) | 2023-01-17 |
| AU2020382121A8 (en) | 2022-07-28 |
| JP2026041819A (ja) | 2026-03-10 |
| US20220112547A1 (en) | 2022-04-14 |
| EP4058599A1 (en) | 2022-09-21 |
| BR112022009072A2 (pt) | 2022-07-26 |
| US11807899B2 (en) | 2023-11-07 |
| KR20220113948A (ko) | 2022-08-17 |
| ZA202205093B (en) | 2024-10-30 |
| WO2021094746A1 (en) | 2021-05-20 |
| US20220145378A1 (en) | 2022-05-12 |
| US12618102B2 (en) | 2026-05-05 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TH | Corrigenda |
Free format text: IN VOL 36 , NO 24 , PAGE(S) 3392 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME BIOCRUCIBLE LIMITED , APPLICATION NO. 2020382121, UNDER INID (71) CORRECT THE APPLICANT NAME TO BIOCRUCIBLE LTD. |