ZA200501871B - Use of saccharomyces cerevisiae erg4 mutants for the expression of glucose transporters from mammals - Google Patents
Use of saccharomyces cerevisiae erg4 mutants for the expression of glucose transporters from mammals Download PDFInfo
- Publication number
- ZA200501871B ZA200501871B ZA2005/01871A ZA200501871A ZA200501871B ZA 200501871 B ZA200501871 B ZA 200501871B ZA 2005/01871 A ZA2005/01871 A ZA 2005/01871A ZA 200501871 A ZA200501871 A ZA 200501871A ZA 200501871 B ZA200501871 B ZA 200501871B
- Authority
- ZA
- South Africa
- Prior art keywords
- protein
- yeast cell
- yeast
- polynucleotide
- glucose
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 86
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title claims description 84
- 108091052347 Glucose transporter family Proteins 0.000 title claims description 31
- 102000042092 Glucose transporter family Human genes 0.000 title claims description 26
- 101100389668 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) erg-4 gene Proteins 0.000 title claims description 19
- 241000124008 Mammalia Species 0.000 title description 5
- 210000005253 yeast cell Anatomy 0.000 claims description 85
- 108090000623 proteins and genes Proteins 0.000 claims description 84
- 102000004169 proteins and genes Human genes 0.000 claims description 74
- 108091033319 polynucleotide Proteins 0.000 claims description 51
- 102000040430 polynucleotide Human genes 0.000 claims description 51
- 239000002157 polynucleotide Substances 0.000 claims description 51
- 150000001875 compounds Chemical class 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 25
- 239000008103 glucose Substances 0.000 claims description 25
- 108091006300 SLC2A4 Proteins 0.000 claims description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 23
- 230000004190 glucose uptake Effects 0.000 claims description 23
- 102000058061 Glucose Transporter Type 4 Human genes 0.000 claims description 21
- 210000004027 cell Anatomy 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 102000058063 Glucose Transporter Type 1 Human genes 0.000 claims description 9
- 108091006296 SLC2A1 Proteins 0.000 claims description 8
- 206010012601 diabetes mellitus Diseases 0.000 claims description 8
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 7
- 239000000654 additive Substances 0.000 claims description 6
- 230000001131 transforming effect Effects 0.000 claims description 6
- 102000018711 Facilitative Glucose Transport Proteins Human genes 0.000 claims description 5
- 230000004936 stimulating effect Effects 0.000 claims description 5
- 101000906283 Homo sapiens Solute carrier family 2, facilitated glucose transporter member 1 Proteins 0.000 claims description 4
- 101000996127 Homo sapiens Solute carrier family 2, facilitated glucose transporter member 4 Proteins 0.000 claims description 4
- 102000052262 human SLC2A4 Human genes 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 101150040602 ERG4 gene Proteins 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 102100031673 Corneodesmosin Human genes 0.000 claims 1
- 101710139375 Corneodesmosin Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 230000005714 functional activity Effects 0.000 claims 1
- 102000052191 human SLC2A1 Human genes 0.000 claims 1
- 239000013598 vector Substances 0.000 description 32
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 18
- 108010078791 Carrier Proteins Proteins 0.000 description 14
- 102000004877 Insulin Human genes 0.000 description 9
- 108090001061 Insulin Proteins 0.000 description 9
- 238000009396 hybridization Methods 0.000 description 9
- 229940125396 insulin Drugs 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- 150000001413 amino acids Chemical group 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 5
- 241000700159 Rattus Species 0.000 description 5
- 150000002402 hexoses Chemical class 0.000 description 5
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000032258 transport Effects 0.000 description 4
- 102000005455 Monosaccharide Transport Proteins Human genes 0.000 description 3
- 108010006769 Monosaccharide Transport Proteins Proteins 0.000 description 3
- 101100507956 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HXT7 gene Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- WQZGKKKJIJFFOK-UKLRSMCWSA-N dextrose-2-13c Chemical compound OC[C@H]1OC(O)[13C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-UKLRSMCWSA-N 0.000 description 3
- 230000006377 glucose transport Effects 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 230000003362 replicative effect Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- 101150030468 MPH3 gene Proteins 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 101100024083 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MPH2 gene Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000005567 liquid scintillation counting Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 206010000188 Abnormal weight gain Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 235000019739 Dicalciumphosphate Nutrition 0.000 description 1
- 101000899738 Drosophila melanogaster Glucose transporter type 3 Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 201000006328 Fanconi syndrome Diseases 0.000 description 1
- 208000037251 Fanconi-Bickel syndrome Diseases 0.000 description 1
- 101150037782 GAL2 gene Proteins 0.000 description 1
- 101710193897 Galactose transporter Proteins 0.000 description 1
- 101710103223 Galactose-proton symporter Proteins 0.000 description 1
- 102100021735 Galectin-2 Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 102100030624 Proton myo-inositol cotransporter Human genes 0.000 description 1
- 108091006310 SLC2A12 Proteins 0.000 description 1
- 108091006309 SLC2A13 Proteins 0.000 description 1
- 108091006299 SLC2A2 Proteins 0.000 description 1
- 101100507949 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HXT2 gene Proteins 0.000 description 1
- 102100023536 Solute carrier family 2, facilitated glucose transporter member 1 Human genes 0.000 description 1
- 102100039671 Solute carrier family 2, facilitated glucose transporter member 12 Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 102000008221 Superoxide Dismutase-1 Human genes 0.000 description 1
- 108010021188 Superoxide Dismutase-1 Proteins 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 241000269370 Xenopus <genus> Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- NEFBYIFKOOEVPA-UHFFFAOYSA-K dicalcium phosphate Chemical compound [Ca+2].[Ca+2].[O-]P([O-])([O-])=O NEFBYIFKOOEVPA-UHFFFAOYSA-K 0.000 description 1
- 229940038472 dicalcium phosphate Drugs 0.000 description 1
- 229910000390 dicalcium phosphate Inorganic materials 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000008298 dragée Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000014101 glucose homeostasis Effects 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 208000019061 glycogen storage disease due to GLUT2 deficiency Diseases 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 230000031852 maintenance of location in cell Effects 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940037201 oris Drugs 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000018883 protein targeting Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 102220034167 rs137853903 Human genes 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000007940 sugar coated tablet Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Diabetes (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Toxicology (AREA)
- Emergency Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Obesity (AREA)
- Plant Pathology (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10242763A DE10242763A1 (de) | 2002-09-14 | 2002-09-14 | Verwendung von Saccharomyces cerevisiae erg4-Mutanten zur Expression von Glukosetransportern aus Säugetieren |
| PCT/EP2003/009812 WO2004026907A2 (de) | 2002-09-14 | 2003-09-04 | Verwendung von saccharomyces cerevisiae erg4-mutanten zur expression von glukosetransportern aus säugetieren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200501871B true ZA200501871B (en) | 2005-10-26 |
Family
ID=31724774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2005/01871A ZA200501871B (en) | 2002-09-14 | 2005-03-04 | Use of saccharomyces cerevisiae erg4 mutants for the expression of glucose transporters from mammals |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP1539958A2 (ja) |
| JP (1) | JP4520854B2 (ja) |
| KR (1) | KR101233998B1 (ja) |
| CN (1) | CN1694960B (ja) |
| AU (1) | AU2003264257B2 (ja) |
| BR (1) | BR0314115A (ja) |
| CA (1) | CA2498636C (ja) |
| DE (1) | DE10242763A1 (ja) |
| HK (1) | HK1084401A1 (ja) |
| IL (2) | IL167321A (ja) |
| MX (1) | MXPA05002816A (ja) |
| NO (1) | NO20051795L (ja) |
| RU (1) | RU2345136C2 (ja) |
| WO (1) | WO2004026907A2 (ja) |
| ZA (1) | ZA200501871B (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899328A (zh) * | 2012-09-28 | 2013-01-30 | 南京农业大学 | 山羊葡萄糖转运载体4基因及其重组表达载体和应用 |
| CN110408616B (zh) * | 2019-07-09 | 2021-06-15 | 中南民族大学 | GLUT4基因敲除的sgRNA、A549细胞系及其构建方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5942398A (en) * | 1998-02-26 | 1999-08-24 | Millennium Pharmaceuticals, Inc. | Nucleic acid molecules encoding glutx and uses thereof |
| AU5477500A (en) * | 1999-06-09 | 2000-12-28 | General Hospital Corporation, The | Method of measuring plasma membrane targeting of glut4 |
| DE10106718A1 (de) * | 2001-02-14 | 2002-09-05 | Aventis Pharma Gmbh | Hefestamm von Saccharomyces cerevisiae mit funktioneller Expression eines Glut-Transporters |
-
2002
- 2002-09-14 DE DE10242763A patent/DE10242763A1/de not_active Withdrawn
-
2003
- 2003-09-04 CA CA2498636A patent/CA2498636C/en not_active Expired - Fee Related
- 2003-09-04 MX MXPA05002816A patent/MXPA05002816A/es active IP Right Grant
- 2003-09-04 CN CN038251485A patent/CN1694960B/zh not_active Expired - Fee Related
- 2003-09-04 WO PCT/EP2003/009812 patent/WO2004026907A2/de active Application Filing
- 2003-09-04 KR KR1020057004292A patent/KR101233998B1/ko not_active IP Right Cessation
- 2003-09-04 EP EP03797264A patent/EP1539958A2/de not_active Withdrawn
- 2003-09-04 BR BR0314115-2A patent/BR0314115A/pt not_active IP Right Cessation
- 2003-09-04 AU AU2003264257A patent/AU2003264257B2/en not_active Ceased
- 2003-09-04 RU RU2005110955/13A patent/RU2345136C2/ru not_active IP Right Cessation
- 2003-09-04 JP JP2004536979A patent/JP4520854B2/ja not_active Expired - Fee Related
-
2005
- 2005-03-04 ZA ZA2005/01871A patent/ZA200501871B/en unknown
- 2005-03-08 IL IL167321A patent/IL167321A/en not_active IP Right Cessation
- 2005-04-12 NO NO20051795A patent/NO20051795L/no not_active Application Discontinuation
-
2006
- 2006-04-18 HK HK06104586.6A patent/HK1084401A1/xx not_active IP Right Cessation
-
2009
- 2009-03-16 IL IL197621A patent/IL197621A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1539958A2 (de) | 2005-06-15 |
| WO2004026907A2 (de) | 2004-04-01 |
| CN1694960A (zh) | 2005-11-09 |
| AU2003264257B2 (en) | 2010-05-20 |
| KR20050056206A (ko) | 2005-06-14 |
| BR0314115A (pt) | 2005-07-12 |
| RU2005110955A (ru) | 2006-01-20 |
| KR101233998B1 (ko) | 2013-02-18 |
| CA2498636A1 (en) | 2004-04-01 |
| CN1694960B (zh) | 2010-05-12 |
| CA2498636C (en) | 2012-04-17 |
| WO2004026907A3 (de) | 2004-11-11 |
| MXPA05002816A (es) | 2005-05-27 |
| IL197621A0 (en) | 2011-08-01 |
| JP4520854B2 (ja) | 2010-08-11 |
| DE10242763A1 (de) | 2004-03-18 |
| RU2345136C2 (ru) | 2009-01-27 |
| JP2006517088A (ja) | 2006-07-20 |
| IL197621A (en) | 2014-01-30 |
| IL167321A (en) | 2013-11-28 |
| HK1084401A1 (en) | 2006-07-28 |
| NO20051795L (no) | 2005-06-08 |
| AU2003264257A1 (en) | 2004-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xu et al. | DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans | |
| US11702627B2 (en) | High cAMP yielding yeast strain and use thereof | |
| Hamada et al. | Isolation and characterization of a novel secretory protein, stromal cell-derived factor-2 (SDF-2) using the signal sequence trap method | |
| CN102816244A (zh) | 一种Exendin-4肽与人血清白蛋白HSA的融合蛋白及其制备方法 | |
| KR20210128742A (ko) | 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 | |
| KR20210041903A (ko) | 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 | |
| US8298812B2 (en) | Use of Saccharomyces cerevisiae erg4 mutants for expressing mammalian glucose transporters | |
| CN111411095B (zh) | 一种重组(R)-ω-转氨酶及其突变体和应用 | |
| JP4287897B2 (ja) | ＧＬＵＴ輸送体が機能的に発現される酵母株Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ | |
| CN113735960B (zh) | 一种fgf重组蛋白治疗nash的应用 | |
| CN1178950C (zh) | 司登尼亚蛋白小体-司登尼亚钙蛋白 | |
| ZA200501871B (en) | Use of saccharomyces cerevisiae erg4 mutants for the expression of glucose transporters from mammals | |
| KR101903553B1 (ko) | 중쇄 아미노카르복시산의 생산 방법 | |
| CN109628476A (zh) | 一种利用全细胞转化生产4-羟基异亮氨酸的方法 | |
| CN115838645A (zh) | 一种高产乳清酸的酵母菌株及其应用 | |
| CN1332240A (zh) | 超氧化物歧化酶－4 | |
| TWI323281B (en) | Saccharomyces cerevisiae yeast strain with functional expression of a glut transporter | |
| KR20070027878A (ko) | 자일리톨의 고수율 생산방법 | |
| TW200411053A (en) | Nucleic acid encoding recombinant salmon calcitionin, expression vector thereof, and method for producing recombinant salmon calcitonin therewith | |
| WO2001031023A1 (fr) | Nouveau polypeptide, proteine precurseur humaine 25 de la nucleoproteine ii, et polynucleotide codant pour ce polypeptide |