YU46304B - Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi - Google Patents

Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi

Info

Publication number
YU46304B
YU46304B YU46386A YU46386A YU46304B YU 46304 B YU46304 B YU 46304B YU 46386 A YU46386 A YU 46386A YU 46386 A YU46386 A YU 46386A YU 46304 B YU46304 B YU 46304B
Authority
YU
Yugoslavia
Prior art keywords
proteins
purification
extraction
produces
procedure
Prior art date
Application number
YU46386A
Other languages
English (en)
Other versions
YU46386A (en
Inventor
F. Van Wijnendael
D. Gilles
G. Simonet
Original Assignee
Smithkline Biologicals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Biologicals filed Critical Smithkline Biologicals
Publication of YU46386A publication Critical patent/YU46386A/xx
Publication of YU46304B publication Critical patent/YU46304B/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

n aznačen time, što se gornji sloj- supernatant ćelija kvasca razorenih u prisustvu ne-jonskog deterdženta u prvoj fazi postupka podesi na pH 6 (+ 0,1) i u ovaj dodaje ili tečan ili čvrst polietilen glikol sve do izbistravanja pomenutog gornjeg sloja, a u drugoj fazi se delimično prečišćen rastvor tretira ili sa dvovalentnim metalnim katjonom, ili posle eventualne ultrafiltracije, sa amonijum sulfatom radi odvajanja pomenutog proteina. Prijava sadrži još 14 zavisnih zahteva.
YU46386A 1985-04-03 1986-03-25 Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi YU46304B (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/719,601 US4683294A (en) 1985-04-03 1985-04-03 Process for the extraction and purification of proteins from culture media producing them

Publications (2)

Publication Number Publication Date
YU46386A YU46386A (en) 1988-02-29
YU46304B true YU46304B (sh) 1993-05-28

Family

ID=24890650

Family Applications (1)

Application Number Title Priority Date Filing Date
YU46386A YU46304B (sh) 1985-04-03 1986-03-25 Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi

Country Status (29)

Country Link
US (2) US4683294A (sh)
EP (1) EP0199698B1 (sh)
JP (1) JP2512430B2 (sh)
KR (1) KR940010283B1 (sh)
CN (1) CN86101798A (sh)
AR (1) AR241596A1 (sh)
AT (1) ATE66694T1 (sh)
AU (1) AU580494B2 (sh)
CA (1) CA1272457A (sh)
CS (1) CS259537B2 (sh)
DE (1) DE3681058D1 (sh)
DK (1) DK152286A (sh)
EG (1) EG17972A (sh)
ES (1) ES8706166A1 (sh)
FI (1) FI85027C (sh)
GR (1) GR860874B (sh)
HU (1) HU206125B (sh)
IE (1) IE58854B1 (sh)
IL (1) IL78824A (sh)
MY (1) MY102936A (sh)
NO (1) NO170421C (sh)
NZ (1) NZ215618A (sh)
PH (1) PH22110A (sh)
PL (1) PL152568B1 (sh)
PT (1) PT82219B (sh)
RO (1) RO95349B (sh)
SU (1) SU1604144A3 (sh)
YU (1) YU46304B (sh)
ZA (1) ZA862374B (sh)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1268437A (en) * 1984-12-21 1990-05-01 Haruo Fujita Method for purification of hbc antigen and method for measurement of hbc antibody by using said purified hbc antigen
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
US5124256A (en) * 1985-11-13 1992-06-23 Labofina, S.A. Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
US4742158A (en) * 1986-04-25 1988-05-03 Merck & Co., Inc. Purification of hepatitis pre-S antigens by polymerized serum albumin affinity binding
FR2608052B1 (fr) * 1986-12-10 1990-04-13 Pasteur Vaccins Procede de preparation d'un vaccin contre l'hepatite b et vaccin obtenu
AP56A (en) * 1987-01-30 1989-09-26 Smithkline Biologicals S A Hepatitis B virus surface antigens and hybrid antigehs containing them.
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
NZ225583A (en) * 1987-08-06 1991-07-26 Merck & Co Inc Process for purifying hepatitis a virions (hav)
US4883865A (en) * 1987-09-30 1989-11-28 Merck & Co. Inc. Recovery of pres2+s antigen
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
US5043287A (en) * 1989-05-16 1991-08-27 The Standard Oil Company Recovery of water soluble biopolymers from an aqueous solution by employing a polyoxide
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
ES2045669T3 (es) * 1989-07-15 1994-01-16 Boehringer Ingelheim Int Procedimiento para la purificacion de anexinas.
US4988798A (en) * 1990-01-22 1991-01-29 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5109121A (en) * 1990-01-22 1992-04-28 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5177194A (en) * 1990-02-01 1993-01-05 Baxter International, Inc. Process for purifying immune serum globulins
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5102989A (en) * 1991-03-15 1992-04-07 Merck & Co., Inc. Method of stabilizing recombinant hepatitis B virus surface proteins from recombinant host cells
US5525519A (en) * 1992-01-07 1996-06-11 Middlesex Sciences, Inc. Method for isolating biomolecules from a biological sample with linear polymers
US6509192B1 (en) * 1992-02-24 2003-01-21 Coulter International Corp. Quality control method
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
KR100254828B1 (ko) * 1994-12-24 2000-05-01 성재갑 재조합 효모로 부터 발현된 b형 간염 항원의 추출 방법
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
UA79735C2 (uk) * 2000-08-10 2007-07-25 Глаксосмітклайн Байолоджікалз С.А. Очищення антигенів вірусу гепатиту b (hbv) для використання у вакцинах
ATE489400T1 (de) 2002-09-06 2010-12-15 Genentech Inc Verfahren zur proteinextraktion
US7777006B2 (en) 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
US8323949B2 (en) * 2003-03-11 2012-12-04 Advanced Biocatalytics Corporation Altering metabolism in biological processes
US7476529B2 (en) * 2003-03-11 2009-01-13 Advanced Biocatalytics Corporation Altering metabolism in biological processes
WO2005027821A2 (en) * 2003-09-22 2005-03-31 Kamada Ltd. Large scale preparation of alpha-1 proteinase inhibitor and use thereof
AU2004309177A1 (en) * 2003-12-30 2005-07-14 Bharat Biotech International Limited A process for the preparation and purification of recombinant proteins
SE0400886D0 (sv) * 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
CN1314447C (zh) * 2004-04-12 2007-05-09 爱华生物科技(香港)有限公司 一种具有抑制蛋白酶作用的药物的制备方法
US8119146B2 (en) 2005-04-18 2012-02-21 Angelica Medina-Selby Expressing hepatitis B virus surface antigen for vaccine preparation
EP1909830B1 (en) * 2005-08-02 2011-09-28 Novartis Vaccines and Diagnostics S.r.l. Reducing interference between oil-containing adjuvants and surfactant-containing antigens
WO2008127305A2 (en) * 2006-11-01 2008-10-23 Biogen Idec Ma Inc. Method of isolating biomacromolecules using low ph and divalent cations
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
EP3670646A1 (en) * 2018-12-21 2020-06-24 Ohly GmbH Functional yeast protein concentrate
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3636191A (en) * 1969-10-08 1972-01-18 Cancer Res Inst Vaccine against viral hepatitis and process
US3790552A (en) * 1972-03-16 1974-02-05 Us Health Method of removing hepatitis-associated antigen from a protein fraction using polyethylene glycol
US3994879A (en) * 1972-12-18 1976-11-30 Hoechst Aktiengesellschaft Process for the preparation of benzofuran compounds
US3951937A (en) * 1973-12-20 1976-04-20 The Community Blood Council Of Greater New York, Inc. Large scale purification of hepatitis type B antigen using polyethylene glycol
US3994870A (en) * 1974-01-31 1976-11-30 The Community Blood Council Of Greater New York, Inc. Purification of hepatitis B surface antigen
SE437329B (sv) * 1975-03-14 1985-02-25 Community Blood Council Sett att ur blodserum framstella vaccin mot virushepatit
NZ180199A (en) * 1976-03-04 1978-11-13 New Zealand Dev Finance Method of testing for the presence of elevated plasma liprotein concentration
US4113712A (en) * 1976-03-08 1978-09-12 The Green Cross Corporation HBsAG Particle composed of single polypeptide subunits and the preparation procedure
GB1589581A (en) * 1976-04-14 1981-05-13 Biotechnolog Forschung Gmbh Process for the separation of enzymes
US4102996A (en) * 1977-04-20 1978-07-25 Merck & Co., Inc. Method of preparing hepatitis B core antigen
DE2750045A1 (de) * 1977-11-09 1979-05-10 Behringwerke Ag Verfahren zur entfernung von detergentien aus virusantigensuspensionen
FR2470795A1 (fr) * 1979-12-07 1981-06-12 Pasteur Institut Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus
US4349539A (en) * 1980-08-15 1982-09-14 Merck & Co., Inc. Separation of hepatitis B surface antigen
US4314997A (en) * 1980-10-06 1982-02-09 Edward Shanbrom Purification of plasma protein products
JPH0625069B2 (ja) * 1981-01-29 1994-04-06 ブリティッシュ・テクノロジー・グループ・リミテッド Ｂ型肝炎ワクチン製造方法
US4542016A (en) * 1981-03-27 1985-09-17 Institut Merieux Non-a non-b hepatitis surface antigen useful for the preparation of vaccines and methods of use
US4329471A (en) * 1981-05-04 1982-05-11 Merrell Dow Pharmaceuticals Inc. 4-(4-Phenyl-1-piperidinyl)methyl-5-acyl-1,3-dihydro-2H-imidazol-2-ones
PL133476B1 (en) * 1981-06-10 1985-06-29 Akad Medyczna Method of obtaining pure antigen hba from human serum
US4379087A (en) * 1982-06-17 1983-04-05 Cutter Laboratories, Inc. Method of preparing alpha-1-proteinase inhibitor
AU577259B2 (en) * 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
AU584580B2 (en) * 1982-09-08 1989-06-01 Smith Kline - Rit Hepatitis B virus vaccine
SE8205892D0 (sv) * 1982-10-18 1982-10-18 Bror Morein Immunogent membranproteinkomplex, sett for framstellning och anvendning derav som immunstimulerande medel och sasom vaccin
JPS59101426A (ja) * 1982-11-29 1984-06-12 Green Cross Corp:The Ｂ型肝炎感染予防用ワクチンの製造方法
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
US4649192A (en) * 1985-05-30 1987-03-10 Smith Kline-Rit Method for the isolation and purification of hepatitis B surface antigen using polysorbate

Also Published As

Publication number Publication date
US4857317A (en) 1989-08-15
IL78824A0 (en) 1986-09-30
PL152568B1 (en) 1991-01-31
US4683294A (en) 1987-07-28
FI861251A (fi) 1986-10-04
NZ215618A (en) 1988-08-30
ATE66694T1 (de) 1991-09-15
AU5378686A (en) 1986-10-09
PH22110A (en) 1988-06-01
ZA862374B (en) 1987-06-24
EP0199698A2 (fr) 1986-10-29
YU46386A (en) 1988-02-29
AR241596A1 (es) 1992-09-30
PT82219A (en) 1986-04-01
JPS61249398A (ja) 1986-11-06
SU1604144A3 (ru) 1990-10-30
IE58854B1 (en) 1993-11-17
RO95349A (ro) 1988-09-15
JP2512430B2 (ja) 1996-07-03
DK152286A (da) 1986-10-04
EP0199698A3 (en) 1989-05-24
HU206125B (en) 1992-08-28
ES553633A0 (es) 1987-06-01
NO861234L (no) 1986-10-06
MY102936A (en) 1993-03-31
CS223386A2 (en) 1988-02-15
RO95349B (ro) 1988-09-16
IL78824A (en) 1991-04-15
CS259537B2 (en) 1988-10-14
FI85027B (fi) 1991-11-15
NO170421B (no) 1992-07-06
CN86101798A (zh) 1986-10-15
IE860826L (en) 1986-10-03
CA1272457A (en) 1990-08-07
ES8706166A1 (es) 1987-06-01
GR860874B (en) 1986-07-30
HUT40810A (en) 1987-02-27
KR860008276A (ko) 1986-11-14
DK152286D0 (da) 1986-04-03
DE3681058D1 (de) 1991-10-02
AU580494B2 (en) 1989-01-12
NO170421C (no) 1992-10-14
KR940010283B1 (ko) 1994-10-22
PT82219B (pt) 1988-02-17
EP0199698B1 (fr) 1991-08-28
EG17972A (en) 1991-06-30
FI861251A0 (fi) 1986-03-24
FI85027C (fi) 1992-02-25

Similar Documents

Publication Publication Date Title
YU46304B (sh) Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi
Butterworth et al. Action of neuraminidase on human kidney alkaline phosphatase
ATE110782T1 (de) Rekombinanter menschlicher endothelzellenwachstumsfaktor.
DE59505786D1 (de) Verfahren zur abreicherung oder entfernung von endotoxinen
ES8106407A1 (es) Procedimiento para el tratamiento de superficies hidrofobas
ES531372A0 (es) Metodo para preparar inmunoglobulinas en celulas hospedantes recombinantes
ES8706263A1 (es) Un metodo para diferenciar subclases de globulos blancos de la sangre
ATE73138T1 (de) Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern.
KR890001581A (ko) 조직인자 억제제
GR3000753T3 (en) Sterile plasma substitute
DE3576362D1 (de) Verfahren zur reinigung einer physiologisch aktiven substanz, die durch rekombinante dna-technik hergestellt wurde.
ATE26986T1 (de) Verfahren zur loesung von peptiden und proteinen, hierdurch erhaltene loesungen und deren verwendung.
ATE120238T1 (de) Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.
JPS57193494A (en) Method of purifying or concentrating biologically active protein and drug therefor
DE3584926D1 (de) Behandlung von oedemen mit hohem proteingehalt durch direktes aufbringen von benzopyronen.
Boyde The interaction between alpha-2-macroglobulin and cationic aspartate aminotransferase.
DE3579733D1 (de) Verfahren zur trennung von tierischen zellen aus fluessiger kultur.
BG60542B2 (en) Water soluble composition having coccidiostatic effect
DE3884798D1 (de) Tripeptide von N-3-4-Methoxyfumaryl-L-2,3-diamino-propansäure und Methode zu deren Herstellung.
JPS559036A (en) Purification of igm
KR910016926A (ko) 인간 β-인터페론의 정제방법
KR840005343A (ko) 항원-항체의 반응에 의해 생물학적 매체로부터 수면-촉진 인자를 분리 또는 정제하는 방법
JPS6480286A (en) Method for purifying enzyme
Nohara Incorporation of [14C] amino acid into protein fractions of rat-liver ribosomes
KR930000681A (ko) 한국형 c형 감염 바이러스의 특이항원인 khcv mbp-코아(core) 17 단백질의 정제 방법