YU46304B - Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi - Google Patents

Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi

Info

Publication number
YU46304B
YU46304B YU46386A YU46386A YU46304B YU 46304 B YU46304 B YU 46304B YU 46386 A YU46386 A YU 46386A YU 46386 A YU46386 A YU 46386A YU 46304 B YU46304 B YU 46304B
Authority
YU
Yugoslavia
Prior art keywords
proteins
purification
extraction
produces
procedure
Prior art date
Application number
YU46386A
Other languages
English (en)
Other versions
YU46386A (en
Inventor
F. Van Wijnendael
D. Gilles
G. Simonet
Original Assignee
Smithkline Biologicals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Biologicals filed Critical Smithkline Biologicals
Publication of YU46386A publication Critical patent/YU46386A/xx
Publication of YU46304B publication Critical patent/YU46304B/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

n aznačen time, što se gornji sloj- supernatant ćelija kvasca razorenih u prisustvu ne-jonskog deterdženta u prvoj fazi postupka podesi na pH 6 (+ 0,1) i u ovaj dodaje ili tečan ili čvrst polietilen glikol sve do izbistravanja pomenutog gornjeg sloja, a u drugoj fazi se delimično prečišćen rastvor tretira ili sa dvovalentnim metalnim katjonom, ili posle eventualne ultrafiltracije, sa amonijum sulfatom radi odvajanja pomenutog proteina. Prijava sadrži još 14 zavisnih zahteva.
YU46386A 1985-04-03 1986-03-25 Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi YU46304B (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/719,601 US4683294A (en) 1985-04-03 1985-04-03 Process for the extraction and purification of proteins from culture media producing them

Publications (2)

Publication Number Publication Date
YU46386A YU46386A (en) 1988-02-29
YU46304B true YU46304B (sh) 1993-05-28

Family

ID=24890650

Family Applications (1)

Application Number Title Priority Date Filing Date
YU46386A YU46304B (sh) 1985-04-03 1986-03-25 Postupak za ekstrakciju i prečišćavanje proteina iz hranljive podloge koja ih proizvodi

Country Status (29)

Country Link
US (2) US4683294A (sh)
EP (1) EP0199698B1 (sh)
JP (1) JP2512430B2 (sh)
KR (1) KR940010283B1 (sh)
CN (1) CN86101798A (sh)
AR (1) AR241596A1 (sh)
AT (1) ATE66694T1 (sh)
AU (1) AU580494B2 (sh)
CA (1) CA1272457A (sh)
CS (1) CS259537B2 (sh)
DE (1) DE3681058D1 (sh)
DK (1) DK152286A (sh)
EG (1) EG17972A (sh)
ES (1) ES8706166A1 (sh)
FI (1) FI85027C (sh)
GR (1) GR860874B (sh)
HU (1) HU206125B (sh)
IE (1) IE58854B1 (sh)
IL (1) IL78824A (sh)
MY (1) MY102936A (sh)
NO (1) NO170421C (sh)
NZ (1) NZ215618A (sh)
PH (1) PH22110A (sh)
PL (1) PL152568B1 (sh)
PT (1) PT82219B (sh)
RO (1) RO95349B (sh)
SU (1) SU1604144A3 (sh)
YU (1) YU46304B (sh)
ZA (1) ZA862374B (sh)

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EP0278940A3 (en) * 1987-01-30 1988-12-07 Smithkline Biologicals S.A. Hepatitis b virus surface antigens and hybrid antigens containing them
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US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
ATE95189T1 (de) * 1989-07-15 1993-10-15 Boehringer Ingelheim Int Verfahren zur reinigung von annexinen.
US5109121A (en) * 1990-01-22 1992-04-28 Pitman-Moore, Inc. Method for recovering recombinant proteins
US4988798A (en) * 1990-01-22 1991-01-29 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5177194A (en) * 1990-02-01 1993-01-05 Baxter International, Inc. Process for purifying immune serum globulins
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
US5102989A (en) * 1991-03-15 1992-04-07 Merck & Co., Inc. Method of stabilizing recombinant hepatitis B virus surface proteins from recombinant host cells
US5525519A (en) * 1992-01-07 1996-06-11 Middlesex Sciences, Inc. Method for isolating biomolecules from a biological sample with linear polymers
US6509192B1 (en) * 1992-02-24 2003-01-21 Coulter International Corp. Quality control method
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
KR100254828B1 (ko) * 1994-12-24 2000-05-01 성재갑 재조합 효모로 부터 발현된 b형 간염 항원의 추출 방법
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
UA79735C2 (uk) * 2000-08-10 2007-07-25 Глаксосмітклайн Байолоджікалз С.А. Очищення антигенів вірусу гепатиту b (hbv) для використання у вакцинах
ES2355088T3 (es) * 2002-09-06 2011-03-22 Genentech, Inc. Proceso para la extracción de proteínas.
US7777006B2 (en) 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
US8323949B2 (en) * 2003-03-11 2012-12-04 Advanced Biocatalytics Corporation Altering metabolism in biological processes
WO2004081034A2 (en) * 2003-03-11 2004-09-23 Advanced Biocatalytics Corporation Altering metabolism in biological processes
ATE391730T1 (de) * 2003-09-22 2008-04-15 Kamada Ltd Herstellung eines alpha-1-proteinaseinhibitors im grossmassstab und dessen verwendung
JP2007535911A (ja) * 2003-12-30 2007-12-13 バハラ バイオテック インターナショナル リミテッド 組換えタンパク質の製造及び精製方法
SE0400886D0 (sv) * 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
CN1314447C (zh) * 2004-04-12 2007-05-09 爱华生物科技(香港)有限公司 一种具有抑制蛋白酶作用的药物的制备方法
CA2605179A1 (en) 2005-04-18 2006-10-26 Novartis Vaccines And Diagnostics, Inc. Expressing hepatitis b virus surface antigen for vaccine preparation
PL1909830T3 (pl) * 2005-08-02 2012-02-29 Novartis Vaccines And Diagnostics S R L Ograniczenie oddziaływania pomiędzy olejem w adiuwancie a środkiem powierzchniowo czynnym w antygenie
EP2078039B1 (en) * 2006-11-01 2017-09-13 Biogen MA Inc. Method of isolating biomacromolecules using low ph and divalent cations
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
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Also Published As

Publication number Publication date
PH22110A (en) 1988-06-01
IL78824A (en) 1991-04-15
CS223386A2 (en) 1988-02-15
US4857317A (en) 1989-08-15
ATE66694T1 (de) 1991-09-15
AU580494B2 (en) 1989-01-12
ZA862374B (en) 1987-06-24
CN86101798A (zh) 1986-10-15
IE860826L (en) 1986-10-03
SU1604144A3 (ru) 1990-10-30
NZ215618A (en) 1988-08-30
KR860008276A (ko) 1986-11-14
DE3681058D1 (de) 1991-10-02
FI861251A (fi) 1986-10-04
YU46386A (en) 1988-02-29
US4683294A (en) 1987-07-28
RO95349B (ro) 1988-09-16
JPS61249398A (ja) 1986-11-06
EP0199698A3 (en) 1989-05-24
FI85027B (fi) 1991-11-15
AU5378686A (en) 1986-10-09
EG17972A (en) 1991-06-30
DK152286A (da) 1986-10-04
FI861251A0 (fi) 1986-03-24
MY102936A (en) 1993-03-31
PT82219B (pt) 1988-02-17
GR860874B (en) 1986-07-30
CS259537B2 (en) 1988-10-14
FI85027C (fi) 1992-02-25
IE58854B1 (en) 1993-11-17
JP2512430B2 (ja) 1996-07-03
RO95349A (ro) 1988-09-15
ES8706166A1 (es) 1987-06-01
NO861234L (no) 1986-10-06
EP0199698B1 (fr) 1991-08-28
HU206125B (en) 1992-08-28
HUT40810A (en) 1987-02-27
IL78824A0 (en) 1986-09-30
ES553633A0 (es) 1987-06-01
NO170421C (no) 1992-10-14
CA1272457A (en) 1990-08-07
EP0199698A2 (fr) 1986-10-29
PT82219A (en) 1986-04-01
PL152568B1 (en) 1991-01-31
DK152286D0 (da) 1986-04-03
KR940010283B1 (ko) 1994-10-22
NO170421B (no) 1992-07-06
AR241596A1 (es) 1992-09-30

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