KR860008276A - 단백질이 생성된 배양기로 부터 그것을 추출, 정제하는 방법 - Google Patents

단백질이 생성된 배양기로 부터 그것을 추출, 정제하는 방법 Download PDF

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KR860008276A
KR860008276A KR1019860002480A KR860002480A KR860008276A KR 860008276 A KR860008276 A KR 860008276A KR 1019860002480 A KR1019860002480 A KR 1019860002480A KR 860002480 A KR860002480 A KR 860002480A KR 860008276 A KR860008276 A KR 860008276A
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protein
polysorbate
treated
gel permeation
polyethylene glycol
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KR1019860002480A
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KR940010283B1 (ko
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비넨달 프란스 반
다니엘 질르
귀 시모네
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스미스 클라인-알 아이 티
제라드 따쎄
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
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    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins

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Abstract

내용 없음

Description

단백질을 생성하는 배양기로 부터 그것을 추출, 정제하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (17)

  1. 세포 결합 단백질을 생성시켜서 비-이온성 세정제의 존재하에 분열시킨, 처리된 효모 세포의 상징액으로 부터 세포-결합 단백질을 추출 및 정제하는 방법에서, 그 공정이, 상징액의 pH를 6(±0.1)로 조정하고, 거기에 상기 상징액이 청정해질때까지 액체나 고체 폴리에틸렌 글리클을 가하여 그 청정한 상징액을 가 금속 양이온으로 처리하거나, 최종적인 한의 여과후, 상기 단백질을 분리하기 위해서 황산 암모늄으로 처리하는 것으로 구성되는 방법.
  2. 제1항에서 액체 폴리에틸렌 글리콜을 10과 35%(체적/체적) 사이로 구성되는 최종농도로 첨가하는 방법.
  3. 제1항에서 상기 폴러에틸렌 글리콜을 최종농도가 6과 12%(체적/체적) 사이로 구성되도록 첨가하는 방법.
  4. 제1항 내지 3항중 어느 것에서, 단백질이 B형 간염 표면 항원인 방법.
  5. 제1항 내지 3항중 어느 것에서, 단백질이 알파-1-항트립신인 방법.
  6. 제1항 내지 15항중 어느 것에서, 청정한 상징액을 2가금속 양이온으로 처리하는 방법.
  7. 제6항에서, 2가 금속양이온이 칼슘이나 마그네슘 양이온인 방법.
  8. 제6 및 7항중 어느 하나에서, 다시 침전의 분리, 용액의 한의 여과, 잔사물의 겔침투, 피크가 포함된 단백질의 이온 교환 크로마토그라피 및 마지막으로 두번째 겔침투나 염화 세슘 구배 원심분리로 이루어지는방법.
  9. 제1항 내지 5항중 어느것에서, 청징한 상징액을 40-50%까지 포화된 황산 암모늄으로 처리하고 폴리에틸렌글리콜상에서 정제된 단백질을 분리하는 방법.
  10. 제9항에서, 폴리에틸렌 글리콜상을 다시 최종적인 한의 여과시킨 다음 겔침투시키고 이온교환 크로마토그라피를 행하는 방법.
  11. 제9항에서, 폴리에틸렌글리콜상을 다시 최종적인 한의 여과시킨 다음 이온 교환 크로마토그라피와 겔 침투를 행하는 방법.
  12. 제2항에서 청정한 상징액을 한의 여과시키고 그 잔류물을 40-50%까지 포화된 환산 암모늄으로 처리하여 적절한 완충액에 넣을 단백질을 침전시키는 방법.
  13. 제12항에서, 다시 한의 여과, 겔침투 및 이온 교환 크로마토그라피로 구성되는 방법.
  14. 제1항 내지 5항중 어느 하나에서 비이온성 세정액이 폴리솔베이트인 방법.
  15. 제14항에서, 폴리솔베이트가 폴리솔베이트 20인 방법.
  16. 15 내지 35%(중량/중량)의 폴리솔베이트를 포함하고 있는 B형 간염 표면항원/폴리솔베이트 혼성 미포.
  17. 제16항에 의한 활성 성분으로서 혼성 미포를 함유하고 있는 B형 간염 왁진.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860002480A 1985-04-03 1986-04-02 단백질이 생성된 배양배지로부터 그 단백질을 추출 및 정제하는 방법 KR940010283B1 (ko)

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Application Number Priority Date Filing Date Title
US719,601 1985-04-03
US06/719,601 US4683294A (en) 1985-04-03 1985-04-03 Process for the extraction and purification of proteins from culture media producing them
US719601 1996-09-25

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EP (1) EP0199698B1 (ko)
JP (1) JP2512430B2 (ko)
KR (1) KR940010283B1 (ko)
CN (1) CN86101798A (ko)
AR (1) AR241596A1 (ko)
AT (1) ATE66694T1 (ko)
AU (1) AU580494B2 (ko)
CA (1) CA1272457A (ko)
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US4857317A (en) 1989-08-15
IL78824A0 (en) 1986-09-30
PL152568B1 (en) 1991-01-31
US4683294A (en) 1987-07-28
FI861251A (fi) 1986-10-04
NZ215618A (en) 1988-08-30
ATE66694T1 (de) 1991-09-15
AU5378686A (en) 1986-10-09
PH22110A (en) 1988-06-01
ZA862374B (en) 1987-06-24
EP0199698A2 (fr) 1986-10-29
YU46304B (sh) 1993-05-28
YU46386A (en) 1988-02-29
AR241596A1 (es) 1992-09-30
PT82219A (en) 1986-04-01
JPS61249398A (ja) 1986-11-06
SU1604144A3 (ru) 1990-10-30
IE58854B1 (en) 1993-11-17
RO95349A (ro) 1988-09-15
JP2512430B2 (ja) 1996-07-03
DK152286A (da) 1986-10-04
EP0199698A3 (en) 1989-05-24
HU206125B (en) 1992-08-28
ES553633A0 (es) 1987-06-01
NO861234L (no) 1986-10-06
MY102936A (en) 1993-03-31
CS223386A2 (en) 1988-02-15
RO95349B (ro) 1988-09-16
IL78824A (en) 1991-04-15
CS259537B2 (en) 1988-10-14
FI85027B (fi) 1991-11-15
NO170421B (no) 1992-07-06
CN86101798A (zh) 1986-10-15
IE860826L (en) 1986-10-03
CA1272457A (en) 1990-08-07
ES8706166A1 (es) 1987-06-01
GR860874B (en) 1986-07-30
HUT40810A (en) 1987-02-27
DK152286D0 (da) 1986-04-03
DE3681058D1 (de) 1991-10-02
AU580494B2 (en) 1989-01-12
NO170421C (no) 1992-10-14
KR940010283B1 (ko) 1994-10-22
PT82219B (pt) 1988-02-17
EP0199698B1 (fr) 1991-08-28
EG17972A (en) 1991-06-30
FI861251A0 (fi) 1986-03-24
FI85027C (fi) 1992-02-25

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