JPS57203015A - Purifying method of human urinary callicrein - Google Patents

Purifying method of human urinary callicrein

Info

Publication number
JPS57203015A
JPS57203015A JP56088287A JP8828781A JPS57203015A JP S57203015 A JPS57203015 A JP S57203015A JP 56088287 A JP56088287 A JP 56088287A JP 8828781 A JP8828781 A JP 8828781A JP S57203015 A JPS57203015 A JP S57203015A
Authority
JP
Japan
Prior art keywords
callicrein
crude
purified
difference
contact
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56088287A
Other languages
Japanese (ja)
Inventor
Takashi Matsumoto
Noboru Ito
Shigeo Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daiichi Kagaku Yakuhin Co Ltd
Daiichi Pure Chemicals Co Ltd
Original Assignee
Daiichi Kagaku Yakuhin Co Ltd
Daiichi Pure Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Kagaku Yakuhin Co Ltd, Daiichi Pure Chemicals Co Ltd filed Critical Daiichi Kagaku Yakuhin Co Ltd
Priority to JP56088287A priority Critical patent/JPS57203015A/en
Publication of JPS57203015A publication Critical patent/JPS57203015A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE: To purify the titled callicrein for the treatment of cerebrovascular disorder highly, by bringing an aqueous solution containing a crude callicrein derived from a human urine into contact with an insoluble carrier containing specific hydrophobic groups bonded thereto, and using the difference in the affinity.
CONSTITUTION: A crude human callicrein is dissolved in a neutral buffer solution in a suitable salt concentration, and then brought into contact with an insoluble carrier containing hydrophobic groups of the formula (R is ≥4C aliphatic or aromatic hydrocarbon), e.g. agarose or spherically treated agarose. Existing impurities are adsorbed on the carrier by the difference in the hydrophobic nature and separated to give the aimed highly purified callicrein in high yield. The resultant purified callicrein is purified to a purity 10W15 times that of the crude callicrein before the purification, and no colored material is contained therein.
COPYRIGHT: (C)1982,JPO&Japio
JP56088287A 1981-06-10 1981-06-10 Purifying method of human urinary callicrein Pending JPS57203015A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56088287A JPS57203015A (en) 1981-06-10 1981-06-10 Purifying method of human urinary callicrein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56088287A JPS57203015A (en) 1981-06-10 1981-06-10 Purifying method of human urinary callicrein

Publications (1)

Publication Number Publication Date
JPS57203015A true JPS57203015A (en) 1982-12-13

Family

ID=13938687

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56088287A Pending JPS57203015A (en) 1981-06-10 1981-06-10 Purifying method of human urinary callicrein

Country Status (1)

Country Link
JP (1) JPS57203015A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62153225A (en) * 1985-12-26 1987-07-08 Sanwa Kagaku Kenkyusho:Kk Purification of swine pancreas kallikrein
JPH01275600A (en) * 1988-04-28 1989-11-06 Green Cross Corp:The Method for purifying antithrombin-iii
EP0546533A2 (en) * 1991-12-10 1993-06-16 Sanwa Kagaku Kenkyusho Co., Ltd. Medical use of kininogenase

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62153225A (en) * 1985-12-26 1987-07-08 Sanwa Kagaku Kenkyusho:Kk Purification of swine pancreas kallikrein
JPH01275600A (en) * 1988-04-28 1989-11-06 Green Cross Corp:The Method for purifying antithrombin-iii
EP0546533A2 (en) * 1991-12-10 1993-06-16 Sanwa Kagaku Kenkyusho Co., Ltd. Medical use of kininogenase
EP0546533A3 (en) * 1991-12-10 1994-03-30 Sanwa Kagaku Kenkyusho Co

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