JPS57203015A - Purifying method of human urinary callicrein - Google Patents
Purifying method of human urinary callicreinInfo
- Publication number
- JPS57203015A JPS57203015A JP56088287A JP8828781A JPS57203015A JP S57203015 A JPS57203015 A JP S57203015A JP 56088287 A JP56088287 A JP 56088287A JP 8828781 A JP8828781 A JP 8828781A JP S57203015 A JPS57203015 A JP S57203015A
- Authority
- JP
- Japan
- Prior art keywords
- callicrein
- crude
- purified
- difference
- contact
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000001399 Kallikrein Human genes 0.000 title abstract 7
- 108060005987 Kallikrein Proteins 0.000 title abstract 7
- 230000002485 urinary effect Effects 0.000 title 1
- 229920000936 Agarose Polymers 0.000 abstract 2
- 125000001165 hydrophobic group Chemical group 0.000 abstract 2
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 208000026106 cerebrovascular disease Diseases 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To purify the titled callicrein for the treatment of cerebrovascular disorder highly, by bringing an aqueous solution containing a crude callicrein derived from a human urine into contact with an insoluble carrier containing specific hydrophobic groups bonded thereto, and using the difference in the affinity.
CONSTITUTION: A crude human callicrein is dissolved in a neutral buffer solution in a suitable salt concentration, and then brought into contact with an insoluble carrier containing hydrophobic groups of the formula (R is ≥4C aliphatic or aromatic hydrocarbon), e.g. agarose or spherically treated agarose. Existing impurities are adsorbed on the carrier by the difference in the hydrophobic nature and separated to give the aimed highly purified callicrein in high yield. The resultant purified callicrein is purified to a purity 10W15 times that of the crude callicrein before the purification, and no colored material is contained therein.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56088287A JPS57203015A (en) | 1981-06-10 | 1981-06-10 | Purifying method of human urinary callicrein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56088287A JPS57203015A (en) | 1981-06-10 | 1981-06-10 | Purifying method of human urinary callicrein |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57203015A true JPS57203015A (en) | 1982-12-13 |
Family
ID=13938687
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56088287A Pending JPS57203015A (en) | 1981-06-10 | 1981-06-10 | Purifying method of human urinary callicrein |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57203015A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62153225A (en) * | 1985-12-26 | 1987-07-08 | Sanwa Kagaku Kenkyusho:Kk | Purification of swine pancreas kallikrein |
JPH01275600A (en) * | 1988-04-28 | 1989-11-06 | Green Cross Corp:The | Method for purifying antithrombin-iii |
EP0546533A2 (en) * | 1991-12-10 | 1993-06-16 | Sanwa Kagaku Kenkyusho Co., Ltd. | Medical use of kininogenase |
-
1981
- 1981-06-10 JP JP56088287A patent/JPS57203015A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62153225A (en) * | 1985-12-26 | 1987-07-08 | Sanwa Kagaku Kenkyusho:Kk | Purification of swine pancreas kallikrein |
JPH01275600A (en) * | 1988-04-28 | 1989-11-06 | Green Cross Corp:The | Method for purifying antithrombin-iii |
EP0546533A2 (en) * | 1991-12-10 | 1993-06-16 | Sanwa Kagaku Kenkyusho Co., Ltd. | Medical use of kininogenase |
EP0546533A3 (en) * | 1991-12-10 | 1994-03-30 | Sanwa Kagaku Kenkyusho Co |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2155477T3 (en) | CHROMATOGRAPHIC PURIFICATION AND NUCLEIC ACID SEPARATION PROCEDURE. | |
ZA842178B (en) | Process and apparatus for the selective extracorporeal separation of blood constituents | |
DE3363395D1 (en) | Process for the preparation of the c1 inactivator and its use | |
EP0103406A3 (en) | Extraction of sucrose | |
SE8402861D0 (en) | PURIFICATION OF BIOLOGICAL MATERIAL | |
ATE17225T1 (en) | METHOD AND DEVICE FOR ANAEROBIC BIOLOGICAL PURIFICATION OF WASTEWATER. | |
JPS57203015A (en) | Purifying method of human urinary callicrein | |
JPS5517364A (en) | Purification of pancreatic kallikrein | |
EP0257873A3 (en) | Method for purifying and isolating two ionic forms of somatomedin c | |
DE3363641D1 (en) | Process for purifying secretin | |
JPS5758629A (en) | Separating and purifying method of colonization stimulating factor and callicrein in human urine | |
JPS55148098A (en) | Concentration and purification of interferon | |
JPS56108798A (en) | Purification of erythropoietin | |
JPS51130800A (en) | Treatment method by exchange,adsorption,separation,enrichment of cesiu m-137 from radio-active waste liquid | |
CA2058453A1 (en) | Chymosin recovery and purification | |
ES2084746T3 (en) | LIGANDS CONTAINING POLYETHERALKYLAMMONY AND POLYETHERALKYLAMINE LINKED TO INORGANIC SUPPORTS AND PROCEDURES FOR USING THEM TO SEPARATE AND CONCENTRATE IONS DESIRED FROM SOLUTIONS. | |
JPS56134987A (en) | Separation and purification of choline oxidase and or betaine aldehyde dehydrogenase | |
JPS56166121A (en) | Purifying method of human blood coagulation factor | |
JPS57140726A (en) | Purification of physiologically active substance having carcinostatic action | |
JPS5795918A (en) | Purification of immunoglobulin | |
JPS56106592A (en) | Purifying method of beta-amylase originating from plant | |
GB2035989B (en) | System for processing used waters by the biological process of activated sludge | |
JPS55167228A (en) | Purification of high-purity kallikrein | |
JPS5668690A (en) | Method for concentration and purification of human fibroplastic interferon | |
JPS5564569A (en) | Purification of thioglycolic acid |