JPS5795918A - Purification of immunoglobulin - Google Patents
Purification of immunoglobulinInfo
- Publication number
- JPS5795918A JPS5795918A JP17183680A JP17183680A JPS5795918A JP S5795918 A JPS5795918 A JP S5795918A JP 17183680 A JP17183680 A JP 17183680A JP 17183680 A JP17183680 A JP 17183680A JP S5795918 A JPS5795918 A JP S5795918A
- Authority
- JP
- Japan
- Prior art keywords
- immunoglobulin
- cation exchange
- placenta
- chloroform
- extraction liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: To obtain high purity immunoglobulin useful as one of the plasma fraction preparations, by treating crude immunoglobulin obtained from extraction liquid of human placenta with chloroform, etc., and then with an adsorbent, and finally purifying with a cation exchange material.
CONSTITUTION: The extraction liquid of the human placenta is mixed with (NH4)2SO4, and the precipitate is removed therefrom. The supernatant liquid is again mixed with (NH4)2SO4, and the resultant crude immunoglobulin is treated with chloroform/ethanol mixture of with chloroform. The treated product is again treated with an adsorbent (e.g. acid clay, activated clay, kaolin, talc, etc.), and the product is purified with a cation exchange material (e,g. cation exchange resin, cation exchange cellulose, etc.) to obtain purified immunoglobulin. The extraction liquid of human placenta used as the starting material is obtained by cutting the placenta blood or placenta to small pueces, and extracting the blood component with physiological saline water.
EFFECT: Low denaturation of immunoglobulin, and high yield.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17183680A JPS5795918A (en) | 1980-12-04 | 1980-12-04 | Purification of immunoglobulin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17183680A JPS5795918A (en) | 1980-12-04 | 1980-12-04 | Purification of immunoglobulin |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5795918A true JPS5795918A (en) | 1982-06-15 |
JPS6364407B2 JPS6364407B2 (en) | 1988-12-12 |
Family
ID=15930650
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17183680A Granted JPS5795918A (en) | 1980-12-04 | 1980-12-04 | Purification of immunoglobulin |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5795918A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021020474A1 (en) * | 2019-07-31 | 2021-02-04 | 協和キリン株式会社 | Method for purifying antibody using adsorbent |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0715203U (en) * | 1993-08-04 | 1995-03-14 | 株式会社荒井製作所 | Oil seal finishing blade |
JPH07299611A (en) * | 1994-05-02 | 1995-11-14 | Nagamori:Kk | Cutting tool for lathe |
-
1980
- 1980-12-04 JP JP17183680A patent/JPS5795918A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021020474A1 (en) * | 2019-07-31 | 2021-02-04 | 協和キリン株式会社 | Method for purifying antibody using adsorbent |
Also Published As
Publication number | Publication date |
---|---|
JPS6364407B2 (en) | 1988-12-12 |
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