JPS5795918A - Purification of immunoglobulin - Google Patents

Purification of immunoglobulin

Info

Publication number
JPS5795918A
JPS5795918A JP17183680A JP17183680A JPS5795918A JP S5795918 A JPS5795918 A JP S5795918A JP 17183680 A JP17183680 A JP 17183680A JP 17183680 A JP17183680 A JP 17183680A JP S5795918 A JPS5795918 A JP S5795918A
Authority
JP
Japan
Prior art keywords
immunoglobulin
cation exchange
placenta
chloroform
extraction liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP17183680A
Other languages
Japanese (ja)
Other versions
JPS6364407B2 (en
Inventor
Koichi Terui
Keiji Tamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SAIKIN KAGAKU KENKYUSHO KK
Original Assignee
SAIKIN KAGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SAIKIN KAGAKU KENKYUSHO KK filed Critical SAIKIN KAGAKU KENKYUSHO KK
Priority to JP17183680A priority Critical patent/JPS5795918A/en
Publication of JPS5795918A publication Critical patent/JPS5795918A/en
Publication of JPS6364407B2 publication Critical patent/JPS6364407B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE: To obtain high purity immunoglobulin useful as one of the plasma fraction preparations, by treating crude immunoglobulin obtained from extraction liquid of human placenta with chloroform, etc., and then with an adsorbent, and finally purifying with a cation exchange material.
CONSTITUTION: The extraction liquid of the human placenta is mixed with (NH4)2SO4, and the precipitate is removed therefrom. The supernatant liquid is again mixed with (NH4)2SO4, and the resultant crude immunoglobulin is treated with chloroform/ethanol mixture of with chloroform. The treated product is again treated with an adsorbent (e.g. acid clay, activated clay, kaolin, talc, etc.), and the product is purified with a cation exchange material (e,g. cation exchange resin, cation exchange cellulose, etc.) to obtain purified immunoglobulin. The extraction liquid of human placenta used as the starting material is obtained by cutting the placenta blood or placenta to small pueces, and extracting the blood component with physiological saline water.
EFFECT: Low denaturation of immunoglobulin, and high yield.
COPYRIGHT: (C)1982,JPO&Japio
JP17183680A 1980-12-04 1980-12-04 Purification of immunoglobulin Granted JPS5795918A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17183680A JPS5795918A (en) 1980-12-04 1980-12-04 Purification of immunoglobulin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17183680A JPS5795918A (en) 1980-12-04 1980-12-04 Purification of immunoglobulin

Publications (2)

Publication Number Publication Date
JPS5795918A true JPS5795918A (en) 1982-06-15
JPS6364407B2 JPS6364407B2 (en) 1988-12-12

Family

ID=15930650

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17183680A Granted JPS5795918A (en) 1980-12-04 1980-12-04 Purification of immunoglobulin

Country Status (1)

Country Link
JP (1) JPS5795918A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020474A1 (en) * 2019-07-31 2021-02-04 協和キリン株式会社 Method for purifying antibody using adsorbent

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0715203U (en) * 1993-08-04 1995-03-14 株式会社荒井製作所 Oil seal finishing blade
JPH07299611A (en) * 1994-05-02 1995-11-14 Nagamori:Kk Cutting tool for lathe

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020474A1 (en) * 2019-07-31 2021-02-04 協和キリン株式会社 Method for purifying antibody using adsorbent

Also Published As

Publication number Publication date
JPS6364407B2 (en) 1988-12-12

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