JPS55167228A - Purification of high-purity kallikrein - Google Patents

Purification of high-purity kallikrein

Info

Publication number
JPS55167228A
JPS55167228A JP7404979A JP7404979A JPS55167228A JP S55167228 A JPS55167228 A JP S55167228A JP 7404979 A JP7404979 A JP 7404979A JP 7404979 A JP7404979 A JP 7404979A JP S55167228 A JPS55167228 A JP S55167228A
Authority
JP
Japan
Prior art keywords
kallikrein
water
purity
insoluble carrier
nnh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7404979A
Other languages
Japanese (ja)
Other versions
JPS6023646B2 (en
Inventor
Seiji Sasaki
Ryohei Yamamoto
Tadahiko Inukai
Hideyuki Hasegawa
Buichi Horio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP54074049A priority Critical patent/JPS6023646B2/en
Publication of JPS55167228A publication Critical patent/JPS55167228A/en
Publication of JPS6023646B2 publication Critical patent/JPS6023646B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To obtain high-purity kallikrein with ease economically useful as a remedy for diseares of cardiovascular system, by purifying kallikrein by means of affinity chromatography using a specific carrier.
CONSTITUTION: A solution of kallikrein is brought into contact with a combination of NH2(CH2)n (n is 2W8) and a water-insoluble carrier wherein an α,ω-diaminoalkane shown by the formula NH2(CH2)nNH2 is linked to the water-insoluble carrier so that kallikrein is adsorbed. Kallikrein is eluted from the adsorbent to give kallikrein-containing eluant. The eluant is then treated with a combination of CH3(CH2)n (n is 1W9) and a water-insoluble carrier wherein an alkylamine shown by the formula CH3(CH2)nNH2 is bonded to the water-insoluble carrier to adsorb kallikrein, kallukrin is eluted from the adsorbent to give high-purity kallikrein. Kallikrein is selectively adsorbed to prevent the inclusion of different proteins.
COPYRIGHT: (C)1980,JPO&Japio
JP54074049A 1979-06-14 1979-06-14 Purification method of high purity kallikrein Expired JPS6023646B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP54074049A JPS6023646B2 (en) 1979-06-14 1979-06-14 Purification method of high purity kallikrein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP54074049A JPS6023646B2 (en) 1979-06-14 1979-06-14 Purification method of high purity kallikrein

Publications (2)

Publication Number Publication Date
JPS55167228A true JPS55167228A (en) 1980-12-26
JPS6023646B2 JPS6023646B2 (en) 1985-06-08

Family

ID=13535928

Family Applications (1)

Application Number Title Priority Date Filing Date
JP54074049A Expired JPS6023646B2 (en) 1979-06-14 1979-06-14 Purification method of high purity kallikrein

Country Status (1)

Country Link
JP (1) JPS6023646B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5973526A (en) * 1982-10-19 1984-04-25 Amano Pharmaceut Co Ltd Fractional purification of protein existing in mammalian brain
WO1995012620A1 (en) * 1993-11-01 1995-05-11 Alpha-Beta Technology, Inc. Derivatized polysaccharide bile acid sequestrant for reducing cholesterol

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5973526A (en) * 1982-10-19 1984-04-25 Amano Pharmaceut Co Ltd Fractional purification of protein existing in mammalian brain
WO1995012620A1 (en) * 1993-11-01 1995-05-11 Alpha-Beta Technology, Inc. Derivatized polysaccharide bile acid sequestrant for reducing cholesterol

Also Published As

Publication number Publication date
JPS6023646B2 (en) 1985-06-08

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