JPS55167228A - Purification of high-purity kallikrein - Google Patents
Purification of high-purity kallikreinInfo
- Publication number
- JPS55167228A JPS55167228A JP7404979A JP7404979A JPS55167228A JP S55167228 A JPS55167228 A JP S55167228A JP 7404979 A JP7404979 A JP 7404979A JP 7404979 A JP7404979 A JP 7404979A JP S55167228 A JPS55167228 A JP S55167228A
- Authority
- JP
- Japan
- Prior art keywords
- kallikrein
- water
- purity
- insoluble carrier
- nnh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To obtain high-purity kallikrein with ease economically useful as a remedy for diseares of cardiovascular system, by purifying kallikrein by means of affinity chromatography using a specific carrier.
CONSTITUTION: A solution of kallikrein is brought into contact with a combination of NH2(CH2)n (n is 2W8) and a water-insoluble carrier wherein an α,ω-diaminoalkane shown by the formula NH2(CH2)nNH2 is linked to the water-insoluble carrier so that kallikrein is adsorbed. Kallikrein is eluted from the adsorbent to give kallikrein-containing eluant. The eluant is then treated with a combination of CH3(CH2)n (n is 1W9) and a water-insoluble carrier wherein an alkylamine shown by the formula CH3(CH2)nNH2 is bonded to the water-insoluble carrier to adsorb kallikrein, kallukrin is eluted from the adsorbent to give high-purity kallikrein. Kallikrein is selectively adsorbed to prevent the inclusion of different proteins.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54074049A JPS6023646B2 (en) | 1979-06-14 | 1979-06-14 | Purification method of high purity kallikrein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54074049A JPS6023646B2 (en) | 1979-06-14 | 1979-06-14 | Purification method of high purity kallikrein |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55167228A true JPS55167228A (en) | 1980-12-26 |
JPS6023646B2 JPS6023646B2 (en) | 1985-06-08 |
Family
ID=13535928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54074049A Expired JPS6023646B2 (en) | 1979-06-14 | 1979-06-14 | Purification method of high purity kallikrein |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6023646B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5973526A (en) * | 1982-10-19 | 1984-04-25 | Amano Pharmaceut Co Ltd | Fractional purification of protein existing in mammalian brain |
WO1995012620A1 (en) * | 1993-11-01 | 1995-05-11 | Alpha-Beta Technology, Inc. | Derivatized polysaccharide bile acid sequestrant for reducing cholesterol |
-
1979
- 1979-06-14 JP JP54074049A patent/JPS6023646B2/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5973526A (en) * | 1982-10-19 | 1984-04-25 | Amano Pharmaceut Co Ltd | Fractional purification of protein existing in mammalian brain |
WO1995012620A1 (en) * | 1993-11-01 | 1995-05-11 | Alpha-Beta Technology, Inc. | Derivatized polysaccharide bile acid sequestrant for reducing cholesterol |
Also Published As
Publication number | Publication date |
---|---|
JPS6023646B2 (en) | 1985-06-08 |
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