JPS5668691A - Method for concentration and purification of human fibroplastic interferon - Google Patents

Method for concentration and purification of human fibroplastic interferon

Info

Publication number
JPS5668691A
JPS5668691A JP14324779A JP14324779A JPS5668691A JP S5668691 A JPS5668691 A JP S5668691A JP 14324779 A JP14324779 A JP 14324779A JP 14324779 A JP14324779 A JP 14324779A JP S5668691 A JPS5668691 A JP S5668691A
Authority
JP
Japan
Prior art keywords
interferon
solution
fibroplastic
contact
carrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP14324779A
Other languages
Japanese (ja)
Inventor
Kazuo Hosoi
Hitoshi Ozawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to JP14324779A priority Critical patent/JPS5668691A/en
Publication of JPS5668691A publication Critical patent/JPS5668691A/en
Pending legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To concentrate and purify the titled substance having an antiviral and antitumor activity to a high concentration, by treating a solution of a crude human fibroplastic interferon into contact with a strongly acidic cation exchanger and then a nickel chelate carrier.
CONSTITUTION: A solution of a crude human fibroplastic interferon is brought into contact with a strongly acidic cation exchanger, e.g. SP-Sephadex C-25 column, under acidic conditions, to adsorb the interferon on the adsorbent. An eluent is used to recover the interferon as a solution. The recovered solution is then brought into contact with a carrier bonded to a chelate group in which nickel is chelated to adsrob the interferon on the carrier. The adsorbed interferon is recovered by an eluent as a solution. According to the method, the concentrating and purifying effect can be more remarkably improved than the method in which the carriers are used alone.
COPYRIGHT: (C)1981,JPO&Japio
JP14324779A 1979-11-07 1979-11-07 Method for concentration and purification of human fibroplastic interferon Pending JPS5668691A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14324779A JPS5668691A (en) 1979-11-07 1979-11-07 Method for concentration and purification of human fibroplastic interferon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14324779A JPS5668691A (en) 1979-11-07 1979-11-07 Method for concentration and purification of human fibroplastic interferon

Publications (1)

Publication Number Publication Date
JPS5668691A true JPS5668691A (en) 1981-06-09

Family

ID=15334301

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14324779A Pending JPS5668691A (en) 1979-11-07 1979-11-07 Method for concentration and purification of human fibroplastic interferon

Country Status (1)

Country Link
JP (1) JPS5668691A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0118808A2 (en) * 1983-03-03 1984-09-19 F. Hoffmann-La Roche Ag Purification of interferon
JPS59167597A (en) * 1983-03-03 1984-09-21 エフ・ホフマン・ラ・ロシユ・ウント・コンパニ−・アクチエンゲゼルシヤフト Purification of recombined interferon

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0118808A2 (en) * 1983-03-03 1984-09-19 F. Hoffmann-La Roche Ag Purification of interferon
JPS59167597A (en) * 1983-03-03 1984-09-21 エフ・ホフマン・ラ・ロシユ・ウント・コンパニ−・アクチエンゲゼルシヤフト Purification of recombined interferon

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