JPS565095A - Purification of kallidinogenase - Google Patents
Purification of kallidinogenaseInfo
- Publication number
- JPS565095A JPS565095A JP7942579A JP7942579A JPS565095A JP S565095 A JPS565095 A JP S565095A JP 7942579 A JP7942579 A JP 7942579A JP 7942579 A JP7942579 A JP 7942579A JP S565095 A JPS565095 A JP S565095A
- Authority
- JP
- Japan
- Prior art keywords
- kallidinogenase
- antibody
- insolubilized
- crude
- eluted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: Crude kallidinogenase is adsorbend on insolubilized kallidnogenase antibody and then eluted to give purified kallidinogenase in high yield.
CONSTITUTION: An antibody which is prepared by using kallidinogenase with desired purity is made to combine covalently with an insoluble carrier to prepare insolubilized kallidinogenase antibody. The resulting antibody is mixed with crude kallidinogenase solution and they are stirred or the crude solution is passed through a column packed with the insolubilized antibody to adsorb the kallidinogenase specifically on the antibody. After the antibody is washed, kallidinogenase is eluted with an appropriate eluting agent.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7942579A JPS565095A (en) | 1979-06-23 | 1979-06-23 | Purification of kallidinogenase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7942579A JPS565095A (en) | 1979-06-23 | 1979-06-23 | Purification of kallidinogenase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS565095A true JPS565095A (en) | 1981-01-20 |
Family
ID=13689504
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7942579A Pending JPS565095A (en) | 1979-06-23 | 1979-06-23 | Purification of kallidinogenase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS565095A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4996560A (en) * | 1988-10-31 | 1991-02-26 | Dainippon Screen Mfg. Co., Ltd. | Apparatus for exposing an image of an original film on a photosensitive material in vacuum contact with the original film, and method therefor |
-
1979
- 1979-06-23 JP JP7942579A patent/JPS565095A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4996560A (en) * | 1988-10-31 | 1991-02-26 | Dainippon Screen Mfg. Co., Ltd. | Apparatus for exposing an image of an original film on a photosensitive material in vacuum contact with the original film, and method therefor |
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