JPS5777959A - Affinitive adsorbent for affnity chromatography - Google Patents

Affinitive adsorbent for affnity chromatography

Info

Publication number
JPS5777959A
JPS5777959A JP55153316A JP15331680A JPS5777959A JP S5777959 A JPS5777959 A JP S5777959A JP 55153316 A JP55153316 A JP 55153316A JP 15331680 A JP15331680 A JP 15331680A JP S5777959 A JPS5777959 A JP S5777959A
Authority
JP
Japan
Prior art keywords
adsorbent
affinitive
adenylyl
adenosine
oligomer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55153316A
Other languages
Japanese (ja)
Inventor
Katsuhiko Matsumoto
Kaoru Fukuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP55153316A priority Critical patent/JPS5777959A/en
Publication of JPS5777959A publication Critical patent/JPS5777959A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE:To obtain an affinitive adsorbent for separation and purification of a specified enzyme or the like by combining[adenylyl-(3' 5')]n-adenosine oligomer used as ligand with a supporting body at an amino position of a base. CONSTITUTION:[Adenylyl-(3' 5')]n-adenosine oligomer (where n is a positive integer) is combined with an agarose derivative having an ester group used as a supporting body through the intermediary of a spacer -CO(CH2)4CH2 or the like, whereby an affinitive adsorbent (having combinations shown by the formula) for affinity chromatography is obtained. This adsorbent is used for specifically separating from other mixtures and purifying protein or enzyme such as polynucleotide phosphorylase capable of combining with[adenylyl-(3' 5')]n-adenosine oligomer which is obtained by adsorbing and separating by using an anion exchange substance from a liquid extracted from crushed bacterial body of Thermus thermophyllus. In this way, a specified enzyme or the like is obtained with high purity and high activity.
JP55153316A 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography Pending JPS5777959A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55153316A JPS5777959A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55153316A JPS5777959A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Publications (1)

Publication Number Publication Date
JPS5777959A true JPS5777959A (en) 1982-05-15

Family

ID=15559822

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55153316A Pending JPS5777959A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Country Status (1)

Country Link
JP (1) JPS5777959A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58193930A (en) * 1982-05-07 1983-11-11 Mitsubishi Electric Corp Multispindle cooler

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NUCLEIC ACID RES=1974 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58193930A (en) * 1982-05-07 1983-11-11 Mitsubishi Electric Corp Multispindle cooler

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