JPS5777958A - Affinitive adsorbent for affnity chromatography - Google Patents

Affinitive adsorbent for affnity chromatography

Info

Publication number
JPS5777958A
JPS5777958A JP55153315A JP15331580A JPS5777958A JP S5777958 A JPS5777958 A JP S5777958A JP 55153315 A JP55153315 A JP 55153315A JP 15331580 A JP15331580 A JP 15331580A JP S5777958 A JPS5777958 A JP S5777958A
Authority
JP
Japan
Prior art keywords
adsorbent
adenosine diphosphate
combining
affinitive
ribose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55153315A
Other languages
Japanese (ja)
Other versions
JPH0154993B2 (en
Inventor
Katsuhiko Matsumoto
Kaoru Fukuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP55153315A priority Critical patent/JPS5777958A/en
Publication of JPS5777958A publication Critical patent/JPS5777958A/en
Publication of JPH0154993B2 publication Critical patent/JPH0154993B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE:To make it possible to separate and purify with excellent purity a substance (enzyme or the like) capable of combining with adenosine diphosphate, by combining adenosine diphosphate used as ligand with a supporting body at specified positions of ribose. CONSTITUTION:Adenosine diphosphate used as ligand is combined with a supporting body such as an agarose derivative through the intermediary of a spacer -(CH2)-6 at positions 2'-C and 3'-C of ribose, whereby an affinitive adsorbent (shown by the formula) for affinity chromatography is prepared. This adsorbent is used, for instance, for separating and purifying, with high purity and without contaminating of other similar proteins or the like, polynucleotide phosphorylase which is an enzyme capable of combining with adenosine diphosphate obtained from a liquid extracted from crushed bacterial body of Thermus thermophyllus through adsorption and separation by using an anion exchange substance.
JP55153315A 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography Granted JPS5777958A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55153315A JPS5777958A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55153315A JPS5777958A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Publications (2)

Publication Number Publication Date
JPS5777958A true JPS5777958A (en) 1982-05-15
JPH0154993B2 JPH0154993B2 (en) 1989-11-21

Family

ID=15559801

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55153315A Granted JPS5777958A (en) 1980-10-31 1980-10-31 Affinitive adsorbent for affnity chromatography

Country Status (1)

Country Link
JP (1) JPS5777958A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58193929A (en) * 1982-05-07 1983-11-11 Mitsubishi Electric Corp Multispindle cooler
WO1995009694A1 (en) * 1993-10-02 1995-04-13 Merck Patent Gmbh Nucleotide-containing sorbent for affinity chromatography

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58193929A (en) * 1982-05-07 1983-11-11 Mitsubishi Electric Corp Multispindle cooler
JPH0565729B2 (en) * 1982-05-07 1993-09-20 Mitsubishi Electric Corp
WO1995009694A1 (en) * 1993-10-02 1995-04-13 Merck Patent Gmbh Nucleotide-containing sorbent for affinity chromatography

Also Published As

Publication number Publication date
JPH0154993B2 (en) 1989-11-21

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