JPS5574793A - Purification of kallikrein - Google Patents
Purification of kallikreinInfo
- Publication number
- JPS5574793A JPS5574793A JP14725478A JP14725478A JPS5574793A JP S5574793 A JPS5574793 A JP S5574793A JP 14725478 A JP14725478 A JP 14725478A JP 14725478 A JP14725478 A JP 14725478A JP S5574793 A JPS5574793 A JP S5574793A
- Authority
- JP
- Japan
- Prior art keywords
- kallikrein
- adsorbent
- water
- adsorbed
- insoluble carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To prepare a high purity kallikrein at a large quantity, by absorbing crude kallikrein to an adsorbent having the structure of NH2-(CH2)n-[water-insoluble carrier], and eluting the adsorbed compound.
CONSTITUTION: A buffer solution containing an α-ω-diaminoalkane of formula NH2(CH2)nNH2 (n is 2W8) is added to a water-insoluble carrier such as agarose or dextran activated with cyanogen bromide, to obtain a bonded product having a formula of NH2(CH2)n-[the water-insoluble carrier]. A solution of crude kallikrein is contacted with the bonded product obtained above, at a pH of about 4.5W8, to adsorb the kallikrein to the adsorbent. The adsorbent adsorbed with the kallikrein is treated with a solution having a pH of about 4.5W8 and containing ≥0.15 M of electrolytes, to elute the adsorbed kallikrein.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147254A JPS5830034B2 (en) | 1978-11-30 | 1978-11-30 | Kallikrein purification method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147254A JPS5830034B2 (en) | 1978-11-30 | 1978-11-30 | Kallikrein purification method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5574793A true JPS5574793A (en) | 1980-06-05 |
JPS5830034B2 JPS5830034B2 (en) | 1983-06-27 |
Family
ID=15426063
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53147254A Expired JPS5830034B2 (en) | 1978-11-30 | 1978-11-30 | Kallikrein purification method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5830034B2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59151732U (en) * | 1983-03-31 | 1984-10-11 | アイシン精機株式会社 | Vehicle pedal vibration prevention device |
JPH0334498Y2 (en) * | 1985-07-30 | 1991-07-22 |
-
1978
- 1978-11-30 JP JP53147254A patent/JPS5830034B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS5830034B2 (en) | 1983-06-27 |
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