JPS5574793A - Purification of kallikrein - Google Patents

Purification of kallikrein

Info

Publication number
JPS5574793A
JPS5574793A JP14725478A JP14725478A JPS5574793A JP S5574793 A JPS5574793 A JP S5574793A JP 14725478 A JP14725478 A JP 14725478A JP 14725478 A JP14725478 A JP 14725478A JP S5574793 A JPS5574793 A JP S5574793A
Authority
JP
Japan
Prior art keywords
kallikrein
adsorbent
water
adsorbed
insoluble carrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14725478A
Other languages
Japanese (ja)
Other versions
JPS5830034B2 (en
Inventor
Seiji Sasaki
Ryohei Yamamoto
Tadahiko Inukai
Hideyuki Hasegawa
Buichi Horio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP53147254A priority Critical patent/JPS5830034B2/en
Publication of JPS5574793A publication Critical patent/JPS5574793A/en
Publication of JPS5830034B2 publication Critical patent/JPS5830034B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To prepare a high purity kallikrein at a large quantity, by absorbing crude kallikrein to an adsorbent having the structure of NH2-(CH2)n-[water-insoluble carrier], and eluting the adsorbed compound.
CONSTITUTION: A buffer solution containing an α-ω-diaminoalkane of formula NH2(CH2)nNH2 (n is 2W8) is added to a water-insoluble carrier such as agarose or dextran activated with cyanogen bromide, to obtain a bonded product having a formula of NH2(CH2)n-[the water-insoluble carrier]. A solution of crude kallikrein is contacted with the bonded product obtained above, at a pH of about 4.5W8, to adsorb the kallikrein to the adsorbent. The adsorbent adsorbed with the kallikrein is treated with a solution having a pH of about 4.5W8 and containing ≥0.15 M of electrolytes, to elute the adsorbed kallikrein.
COPYRIGHT: (C)1980,JPO&Japio
JP53147254A 1978-11-30 1978-11-30 Kallikrein purification method Expired JPS5830034B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53147254A JPS5830034B2 (en) 1978-11-30 1978-11-30 Kallikrein purification method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53147254A JPS5830034B2 (en) 1978-11-30 1978-11-30 Kallikrein purification method

Publications (2)

Publication Number Publication Date
JPS5574793A true JPS5574793A (en) 1980-06-05
JPS5830034B2 JPS5830034B2 (en) 1983-06-27

Family

ID=15426063

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53147254A Expired JPS5830034B2 (en) 1978-11-30 1978-11-30 Kallikrein purification method

Country Status (1)

Country Link
JP (1) JPS5830034B2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59151732U (en) * 1983-03-31 1984-10-11 アイシン精機株式会社 Vehicle pedal vibration prevention device
JPH0334498Y2 (en) * 1985-07-30 1991-07-22

Also Published As

Publication number Publication date
JPS5830034B2 (en) 1983-06-27

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