JPS5581587A - Purification of kallikrein - Google Patents

Purification of kallikrein

Info

Publication number
JPS5581587A
JPS5581587A JP15420178A JP15420178A JPS5581587A JP S5581587 A JPS5581587 A JP S5581587A JP 15420178 A JP15420178 A JP 15420178A JP 15420178 A JP15420178 A JP 15420178A JP S5581587 A JPS5581587 A JP S5581587A
Authority
JP
Japan
Prior art keywords
kallikrein
adsorbed
alkylamine
formula
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15420178A
Other languages
Japanese (ja)
Other versions
JPS5838150B2 (en
Inventor
Seiji Sasaki
Ryohei Yamamoto
Tadahiko Inukai
Hideyuki Hasegawa
Buichi Horio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP53154201A priority Critical patent/JPS5838150B2/en
Publication of JPS5581587A publication Critical patent/JPS5581587A/en
Publication of JPS5838150B2 publication Critical patent/JPS5838150B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE: Kallikrein is adsorbed on a water-insoluble carrier combined with an alkylamine and purified simply in a large scale up to high purity.
CONSTITUTION: An alkylamine of formula I (n is 1W9) is bonded to a water- insoluble and hydrophilic carrier which is inactive and rough, such as agarose, dextrin, cellulose powder, to give the combined product of formula II (n is the same as above). A liquid containing kallikrein is brought into contact with the combined product preferably at 4.5W6pH and kallidrein is selectively adsorbed on it. Then, the adsorbed kallikrein is eluted with a solution containing an electrolyte as sodium chloride at 4.5W6pH or containing a hydrophobic compound as n-propanol.
COPYRIGHT: (C)1980,JPO&Japio
JP53154201A 1978-12-15 1978-12-15 Kallikrein purification method Expired JPS5838150B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53154201A JPS5838150B2 (en) 1978-12-15 1978-12-15 Kallikrein purification method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53154201A JPS5838150B2 (en) 1978-12-15 1978-12-15 Kallikrein purification method

Publications (2)

Publication Number Publication Date
JPS5581587A true JPS5581587A (en) 1980-06-19
JPS5838150B2 JPS5838150B2 (en) 1983-08-20

Family

ID=15579043

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53154201A Expired JPS5838150B2 (en) 1978-12-15 1978-12-15 Kallikrein purification method

Country Status (1)

Country Link
JP (1) JPS5838150B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6257254U (en) * 1985-09-26 1987-04-09

Also Published As

Publication number Publication date
JPS5838150B2 (en) 1983-08-20

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