JPS5476893A - Novel preparation of chlesterol oxidase - Google Patents

Novel preparation of chlesterol oxidase

Info

Publication number
JPS5476893A
JPS5476893A JP14382977A JP14382977A JPS5476893A JP S5476893 A JPS5476893 A JP S5476893A JP 14382977 A JP14382977 A JP 14382977A JP 14382977 A JP14382977 A JP 14382977A JP S5476893 A JPS5476893 A JP S5476893A
Authority
JP
Japan
Prior art keywords
cholesterol
oxidase
cholesterol oxidase
chlesterol
floating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14382977A
Other languages
Japanese (ja)
Other versions
JPS6120268B2 (en
Inventor
Akinori Matsuzaki
Toshio Kamei
Hajime Suzuki
Yoshiko Takiguchi
Akishiro Nakamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MSD KK
Original Assignee
Banyu Phamaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Banyu Phamaceutical Co Ltd filed Critical Banyu Phamaceutical Co Ltd
Priority to JP14382977A priority Critical patent/JPS5476893A/en
Publication of JPS5476893A publication Critical patent/JPS5476893A/en
Publication of JPS6120268B2 publication Critical patent/JPS6120268B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To separate pure cholesteroloxidase, easily, from an enzyme solution containing cholesterol oxidase, by the use of cholesterol as adsorbent.
CONSTITUTION: Floating cholesterol (floating on water surface) is converted to precipitated cholesterol by heating. An enzymatic solution containing cholesterol oxidase (medium obtained by culturing cholesterol oxidase-producing microbials) is treated with the above precipitated cholesterol, and adsorbed thereto. The adsorbed cholesterol oxidase is washed, eluted with a surface active agent, and purified to afford the pure cholesterol oxidase.
COPYRIGHT: (C)1979,JPO&Japio
JP14382977A 1977-12-02 1977-12-02 Novel preparation of chlesterol oxidase Granted JPS5476893A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14382977A JPS5476893A (en) 1977-12-02 1977-12-02 Novel preparation of chlesterol oxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14382977A JPS5476893A (en) 1977-12-02 1977-12-02 Novel preparation of chlesterol oxidase

Publications (2)

Publication Number Publication Date
JPS5476893A true JPS5476893A (en) 1979-06-19
JPS6120268B2 JPS6120268B2 (en) 1986-05-21

Family

ID=15347906

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14382977A Granted JPS5476893A (en) 1977-12-02 1977-12-02 Novel preparation of chlesterol oxidase

Country Status (1)

Country Link
JP (1) JPS5476893A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS565094A (en) * 1979-06-20 1981-01-20 Boehringer Mannheim Gmbh Production of cholesterine oxidase
JPS5878586A (en) * 1981-10-19 1983-05-12 イ−ストマン・コダツク・カンパニ− Purification of cholesterol oxidase
FR2726912A1 (en) * 1994-11-15 1996-05-15 Asahi Chemical Ind METHOD FOR DETERMINING ANTISTREPTOLYSINE O ANTIBODY
US11549090B2 (en) 2016-08-21 2023-01-10 Adva Biotechnology Ltd. Bioreactor and methods of use thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS565094A (en) * 1979-06-20 1981-01-20 Boehringer Mannheim Gmbh Production of cholesterine oxidase
JPS6012029B2 (en) * 1979-06-20 1985-03-29 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Production method of cholesterin oxidase
JPS5878586A (en) * 1981-10-19 1983-05-12 イ−ストマン・コダツク・カンパニ− Purification of cholesterol oxidase
FR2726912A1 (en) * 1994-11-15 1996-05-15 Asahi Chemical Ind METHOD FOR DETERMINING ANTISTREPTOLYSINE O ANTIBODY
US11549090B2 (en) 2016-08-21 2023-01-10 Adva Biotechnology Ltd. Bioreactor and methods of use thereof
US11859163B2 (en) 2016-08-21 2024-01-02 Adva Biotechnology Ltd. Method of using a bioreactor

Also Published As

Publication number Publication date
JPS6120268B2 (en) 1986-05-21

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