JPS5566525A - Fractionation of compound having cis-type diol structure - Google Patents
Fractionation of compound having cis-type diol structureInfo
- Publication number
- JPS5566525A JPS5566525A JP14002378A JP14002378A JPS5566525A JP S5566525 A JPS5566525 A JP S5566525A JP 14002378 A JP14002378 A JP 14002378A JP 14002378 A JP14002378 A JP 14002378A JP S5566525 A JPS5566525 A JP S5566525A
- Authority
- JP
- Japan
- Prior art keywords
- cis
- type diol
- polymer
- diol structure
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
PURPOSE: To carry out the fractional elution of a compound having, cis-type diol structure, easily, by the column chromatography using a column packed with a specific polymer, while changing the pH of eluents gradually from an alkaline side to an acidic side.
CONSTITUTION: A column packed with a water-insoluble polymer having dihydroxyboronly group [e.g. boron-containing polymer having a unit of formula (R1 is H or methyl; R2 is H or lower alkyl; n is an integer of 1W3), etc.] is used. After it is activated with an alkali, a mixed solution which contains compounds having cis-type diol structure is adsorbed thereon. Subsequently, the column is washed with an acid, and column chromatography is carried out while changing the pH of eluents gradually from an alkaline side to an acidic side, whereby each constituent in said mixed solution is eluted fractionally. The ability of said polymer to form a complex with cis-type diol group is righer on an alkaline side and lower on an acidic side.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14002378A JPS5566525A (en) | 1978-11-14 | 1978-11-14 | Fractionation of compound having cis-type diol structure |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14002378A JPS5566525A (en) | 1978-11-14 | 1978-11-14 | Fractionation of compound having cis-type diol structure |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5566525A true JPS5566525A (en) | 1980-05-20 |
Family
ID=15259139
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP14002378A Pending JPS5566525A (en) | 1978-11-14 | 1978-11-14 | Fractionation of compound having cis-type diol structure |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5566525A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60209596A (en) * | 1984-03-22 | 1985-10-22 | イナルコ エスピーエイ | Lactalose purification |
WO1995020594A1 (en) * | 1994-01-27 | 1995-08-03 | The University Of Strathclyde | Purification of plant-derived polynucleotides |
-
1978
- 1978-11-14 JP JP14002378A patent/JPS5566525A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60209596A (en) * | 1984-03-22 | 1985-10-22 | イナルコ エスピーエイ | Lactalose purification |
JPH0545599B2 (en) * | 1984-03-22 | 1993-07-09 | Inaruko Spa | |
WO1995020594A1 (en) * | 1994-01-27 | 1995-08-03 | The University Of Strathclyde | Purification of plant-derived polynucleotides |
US5969129A (en) * | 1994-01-27 | 1999-10-19 | The University Of Strathclyde | Purification of polynucleotides from polynucleotide/polysaccharide mixtures |
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