JPS55148098A - Concentration and purification of interferon - Google Patents
Concentration and purification of interferonInfo
- Publication number
- JPS55148098A JPS55148098A JP5630379A JP5630379A JPS55148098A JP S55148098 A JPS55148098 A JP S55148098A JP 5630379 A JP5630379 A JP 5630379A JP 5630379 A JP5630379 A JP 5630379A JP S55148098 A JPS55148098 A JP S55148098A
- Authority
- JP
- Japan
- Prior art keywords
- interferon
- metal chelate
- cell
- chelate column
- histyn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To prepare concentrated and purified interferon, by removing histidine, histyn and/or cysteine from crude interferon solution obtained by culturing, and subjecting the product to metal chelate column chromatography.
CONSTITUTION: Crude interferon liquid capable of combining a metal chelate, and essentially free from histidine, histyn and/or cysteine, e.g. interferon derived from human fibroblast, interferon produced by a established strain of cultured cell such as MG-258 cell, MG-63 cell, or interferon produced by namarva cell, is subjected to a metal chelate column chromatography pref. having Zn or Cu ion as the central atom to adsorb the interferon to the metal chelate column.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5630379A JPS55148098A (en) | 1979-05-10 | 1979-05-10 | Concentration and purification of interferon |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5630379A JPS55148098A (en) | 1979-05-10 | 1979-05-10 | Concentration and purification of interferon |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS55148098A true JPS55148098A (en) | 1980-11-18 |
Family
ID=13023355
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5630379A Pending JPS55148098A (en) | 1979-05-10 | 1979-05-10 | Concentration and purification of interferon |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55148098A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0110302A2 (en) * | 1982-11-24 | 1984-06-13 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Method for producing monomeric interferons |
US4569790A (en) * | 1984-03-28 | 1986-02-11 | Cetus Corporation | Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions |
JPS61148197A (en) * | 1984-12-05 | 1986-07-05 | イ−ライ・リリ−・アンド・カンパニ− | Improvement in protein purification |
US4908434A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Process for preparing purified interleukin-2 |
US4908433A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Uses of interleukin-2 |
US4925919A (en) * | 1984-04-25 | 1990-05-15 | Roland Mertelsmann | Purified interleukin 2 |
CN109675535A (en) * | 2018-12-18 | 2019-04-26 | 杨小丽 | Immobilization zinc ion affinity chromatographic material and its preparation method and application |
-
1979
- 1979-05-10 JP JP5630379A patent/JPS55148098A/en active Pending
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0110302A2 (en) * | 1982-11-24 | 1984-06-13 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Method for producing monomeric interferons |
US4569790A (en) * | 1984-03-28 | 1986-02-11 | Cetus Corporation | Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions |
US5419899A (en) * | 1984-03-28 | 1995-05-30 | Koths; Kirston | Purified, reduced recombinant interleukin-2 compositions |
US5614185A (en) * | 1984-03-28 | 1997-03-25 | Chiron Corporation | Compositions containing reduced, microbially produced interleukin-2 (IL-2) |
US4908434A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Process for preparing purified interleukin-2 |
US4908433A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Uses of interleukin-2 |
US4925919A (en) * | 1984-04-25 | 1990-05-15 | Roland Mertelsmann | Purified interleukin 2 |
JPS61148197A (en) * | 1984-12-05 | 1986-07-05 | イ−ライ・リリ−・アンド・カンパニ− | Improvement in protein purification |
CN109675535A (en) * | 2018-12-18 | 2019-04-26 | 杨小丽 | Immobilization zinc ion affinity chromatographic material and its preparation method and application |
CN109675535B (en) * | 2018-12-18 | 2021-10-22 | 杨小丽 | Immobilized zinc ion affinity chromatography material and preparation method and application thereof |
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