JPS55148098A - Concentration and purification of interferon - Google Patents

Concentration and purification of interferon

Info

Publication number
JPS55148098A
JPS55148098A JP5630379A JP5630379A JPS55148098A JP S55148098 A JPS55148098 A JP S55148098A JP 5630379 A JP5630379 A JP 5630379A JP 5630379 A JP5630379 A JP 5630379A JP S55148098 A JPS55148098 A JP S55148098A
Authority
JP
Japan
Prior art keywords
interferon
metal chelate
cell
chelate column
histyn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5630379A
Other languages
Japanese (ja)
Inventor
Kazuo Hosoi
Hitoshi Ozawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to JP5630379A priority Critical patent/JPS55148098A/en
Publication of JPS55148098A publication Critical patent/JPS55148098A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: To prepare concentrated and purified interferon, by removing histidine, histyn and/or cysteine from crude interferon solution obtained by culturing, and subjecting the product to metal chelate column chromatography.
CONSTITUTION: Crude interferon liquid capable of combining a metal chelate, and essentially free from histidine, histyn and/or cysteine, e.g. interferon derived from human fibroblast, interferon produced by a established strain of cultured cell such as MG-258 cell, MG-63 cell, or interferon produced by namarva cell, is subjected to a metal chelate column chromatography pref. having Zn or Cu ion as the central atom to adsorb the interferon to the metal chelate column.
COPYRIGHT: (C)1980,JPO&Japio
JP5630379A 1979-05-10 1979-05-10 Concentration and purification of interferon Pending JPS55148098A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5630379A JPS55148098A (en) 1979-05-10 1979-05-10 Concentration and purification of interferon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5630379A JPS55148098A (en) 1979-05-10 1979-05-10 Concentration and purification of interferon

Publications (1)

Publication Number Publication Date
JPS55148098A true JPS55148098A (en) 1980-11-18

Family

ID=13023355

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5630379A Pending JPS55148098A (en) 1979-05-10 1979-05-10 Concentration and purification of interferon

Country Status (1)

Country Link
JP (1) JPS55148098A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0110302A2 (en) * 1982-11-24 1984-06-13 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Method for producing monomeric interferons
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
JPS61148197A (en) * 1984-12-05 1986-07-05 イ−ライ・リリ−・アンド・カンパニ− Improvement in protein purification
US4908434A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Process for preparing purified interleukin-2
US4908433A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Uses of interleukin-2
US4925919A (en) * 1984-04-25 1990-05-15 Roland Mertelsmann Purified interleukin 2
CN109675535A (en) * 2018-12-18 2019-04-26 杨小丽 Immobilization zinc ion affinity chromatographic material and its preparation method and application

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0110302A2 (en) * 1982-11-24 1984-06-13 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Method for producing monomeric interferons
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US5419899A (en) * 1984-03-28 1995-05-30 Koths; Kirston Purified, reduced recombinant interleukin-2 compositions
US5614185A (en) * 1984-03-28 1997-03-25 Chiron Corporation Compositions containing reduced, microbially produced interleukin-2 (IL-2)
US4908434A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Process for preparing purified interleukin-2
US4908433A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Uses of interleukin-2
US4925919A (en) * 1984-04-25 1990-05-15 Roland Mertelsmann Purified interleukin 2
JPS61148197A (en) * 1984-12-05 1986-07-05 イ−ライ・リリ−・アンド・カンパニ− Improvement in protein purification
CN109675535A (en) * 2018-12-18 2019-04-26 杨小丽 Immobilization zinc ion affinity chromatographic material and its preparation method and application
CN109675535B (en) * 2018-12-18 2021-10-22 杨小丽 Immobilized zinc ion affinity chromatography material and preparation method and application thereof

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