ES8706166A1 - Un procedimiento para extraer y purificar una proteina fijada a celulas - Google Patents

Un procedimiento para extraer y purificar una proteina fijada a celulas

Info

Publication number
ES8706166A1
ES8706166A1 ES553633A ES553633A ES8706166A1 ES 8706166 A1 ES8706166 A1 ES 8706166A1 ES 553633 A ES553633 A ES 553633A ES 553633 A ES553633 A ES 553633A ES 8706166 A1 ES8706166 A1 ES 8706166A1
Authority
ES
Spain
Prior art keywords
proteins
purification
extraction
culture media
media producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES553633A
Other languages
English (en)
Other versions
ES553633A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline RIT
Original Assignee
SmithKline RIT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline RIT filed Critical SmithKline RIT
Publication of ES553633A0 publication Critical patent/ES553633A0/es
Publication of ES8706166A1 publication Critical patent/ES8706166A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins

Abstract

METODO DE EXTRACCION Y PURIFICACION DE PROTEINAS FIJADAS A CELULAS. CONSISTE EN AJUSTAR A 6G0,1 EL PH DEL SOBRENADANTE DE CELULAS DE LEVADURA OBTENIDAS ARTIFICIALMENTE, QUE HAN PRODUCIDO LA PROTEINA ALFA-1-ANTITRIPSINA O EL ANTIGENO DE SUPERFICIE DE LA HEPATITIS B Y QUE SE HAN ROTO EN PRESENCIA DE UN DETERGENTE NO IONICO; TRATAR CON POLIETILENGLICOL HASTA CLARIFICACION DEL SOBRENADANTE Y, A CONTINUACION, CON UN CATION METALICO DIVALENTE, TAL COMO CALCIO O MAGNESIO, O BIEN CON SULFATO AMONICO DESPUES DE ULTRAFILTRACION. TIENE APLICACIONES PARA LA EXTRACCION Y PURIFICACION DE ANTIGENO DE SUPERFICIE DEL VIRUS DE LA HEPATITIS B Y DE ALFA-1-ANTITRIPSINA PRODUCIDOS POR TECNICAS DE ADN RECOMBINANTE.
ES553633A 1985-04-03 1986-04-02 Un procedimiento para extraer y purificar una proteina fijada a celulas Expired ES8706166A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/719,601 US4683294A (en) 1985-04-03 1985-04-03 Process for the extraction and purification of proteins from culture media producing them

Publications (2)

Publication Number Publication Date
ES553633A0 ES553633A0 (es) 1987-06-01
ES8706166A1 true ES8706166A1 (es) 1987-06-01

Family

ID=24890650

Family Applications (1)

Application Number Title Priority Date Filing Date
ES553633A Expired ES8706166A1 (es) 1985-04-03 1986-04-02 Un procedimiento para extraer y purificar una proteina fijada a celulas

Country Status (29)

Country Link
US (2) US4683294A (es)
EP (1) EP0199698B1 (es)
JP (1) JP2512430B2 (es)
KR (1) KR940010283B1 (es)
CN (1) CN86101798A (es)
AR (1) AR241596A1 (es)
AT (1) ATE66694T1 (es)
AU (1) AU580494B2 (es)
CA (1) CA1272457A (es)
CS (1) CS259537B2 (es)
DE (1) DE3681058D1 (es)
DK (1) DK152286A (es)
EG (1) EG17972A (es)
ES (1) ES8706166A1 (es)
FI (1) FI85027C (es)
GR (1) GR860874B (es)
HU (1) HU206125B (es)
IE (1) IE58854B1 (es)
IL (1) IL78824A (es)
MY (1) MY102936A (es)
NO (1) NO170421C (es)
NZ (1) NZ215618A (es)
PH (1) PH22110A (es)
PL (1) PL152568B1 (es)
PT (1) PT82219B (es)
RO (1) RO95349B (es)
SU (1) SU1604144A3 (es)
YU (1) YU46304B (es)
ZA (1) ZA862374B (es)

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CA1268437A (en) * 1984-12-21 1990-05-01 Haruo Fujita Method for purification of hbc antigen and method for measurement of hbc antibody by using said purified hbc antigen
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
US5124256A (en) * 1985-11-13 1992-06-23 Labofina, S.A. Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
US4742158A (en) * 1986-04-25 1988-05-03 Merck & Co., Inc. Purification of hepatitis pre-S antigens by polymerized serum albumin affinity binding
FR2608052B1 (fr) * 1986-12-10 1990-04-13 Pasteur Vaccins Procede de preparation d'un vaccin contre l'hepatite b et vaccin obtenu
EP0278940A3 (en) * 1987-01-30 1988-12-07 Smithkline Biologicals S.A. Hepatitis b virus surface antigens and hybrid antigens containing them
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
NZ225583A (en) * 1987-08-06 1991-07-26 Merck & Co Inc Process for purifying hepatitis a virions (hav)
US4883865A (en) * 1987-09-30 1989-11-28 Merck & Co. Inc. Recovery of pres2+s antigen
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
US5043287A (en) * 1989-05-16 1991-08-27 The Standard Oil Company Recovery of water soluble biopolymers from an aqueous solution by employing a polyoxide
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
DE4021979A1 (de) * 1989-07-15 1991-01-31 Boehringer Ingelheim Int Verfahren zur reinigung von annexinen
US5109121A (en) * 1990-01-22 1992-04-28 Pitman-Moore, Inc. Method for recovering recombinant proteins
US4988798A (en) * 1990-01-22 1991-01-29 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5177194A (en) * 1990-02-01 1993-01-05 Baxter International, Inc. Process for purifying immune serum globulins
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5102989A (en) * 1991-03-15 1992-04-07 Merck & Co., Inc. Method of stabilizing recombinant hepatitis B virus surface proteins from recombinant host cells
US5525519A (en) * 1992-01-07 1996-06-11 Middlesex Sciences, Inc. Method for isolating biomolecules from a biological sample with linear polymers
US6509192B1 (en) * 1992-02-24 2003-01-21 Coulter International Corp. Quality control method
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
KR100254828B1 (ko) * 1994-12-24 2000-05-01 성재갑 재조합 효모로 부터 발현된 b형 간염 항원의 추출 방법
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
UA79735C2 (uk) 2000-08-10 2007-07-25 Глаксосмітклайн Байолоджікалз С.А. Очищення антигенів вірусу гепатиту b (hbv) для використання у вакцинах
DK1539798T3 (da) 2002-09-06 2011-02-28 Genentech Inc Fremgangsmåde til proteinekstraktion
US7777006B2 (en) 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
WO2004081034A2 (en) * 2003-03-11 2004-09-23 Advanced Biocatalytics Corporation Altering metabolism in biological processes
US8323949B2 (en) * 2003-03-11 2012-12-04 Advanced Biocatalytics Corporation Altering metabolism in biological processes
EP1664123B2 (en) * 2003-09-22 2011-11-30 Kamada Ltd. Large scale preparation of alpha-1 proteinase inhibitor and use thereof
JP2007535911A (ja) * 2003-12-30 2007-12-13 バハラ バイオテック インターナショナル リミテッド 組換えタンパク質の製造及び精製方法
SE0400886D0 (sv) * 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
CN1314447C (zh) * 2004-04-12 2007-05-09 爱华生物科技(香港)有限公司 一种具有抑制蛋白酶作用的药物的制备方法
SG175456A1 (en) 2005-04-18 2011-11-28 Novartis Vaccines & Diagnostic Expressing hepatitis b virus surface antigen for vaccine preparation
CN103690945A (zh) * 2005-08-02 2014-04-02 诺华疫苗和诊断有限公司 降低含油佐剂和含表面活性剂抗原之间的干扰
US9109015B2 (en) * 2006-11-01 2015-08-18 Biogen Ma Inc Method of isolating biomacromolecules using low pH and divalent cations
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
EP3670646A1 (en) * 2018-12-21 2020-06-24 Ohly GmbH Functional yeast protein concentrate

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US3790552A (en) * 1972-03-16 1974-02-05 Us Health Method of removing hepatitis-associated antigen from a protein fraction using polyethylene glycol
US3994879A (en) * 1972-12-18 1976-11-30 Hoechst Aktiengesellschaft Process for the preparation of benzofuran compounds
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Also Published As

Publication number Publication date
PT82219A (en) 1986-04-01
JP2512430B2 (ja) 1996-07-03
RO95349B (ro) 1988-09-16
CS259537B2 (en) 1988-10-14
ZA862374B (en) 1987-06-24
JPS61249398A (ja) 1986-11-06
HU206125B (en) 1992-08-28
IE860826L (en) 1986-10-03
FI861251A (fi) 1986-10-04
FI85027B (fi) 1991-11-15
RO95349A (ro) 1988-09-15
KR940010283B1 (ko) 1994-10-22
CN86101798A (zh) 1986-10-15
AU580494B2 (en) 1989-01-12
IL78824A0 (en) 1986-09-30
IL78824A (en) 1991-04-15
ES553633A0 (es) 1987-06-01
EP0199698A3 (en) 1989-05-24
FI861251A0 (fi) 1986-03-24
FI85027C (fi) 1992-02-25
YU46386A (en) 1988-02-29
PT82219B (pt) 1988-02-17
CA1272457A (en) 1990-08-07
AR241596A1 (es) 1992-09-30
YU46304B (sh) 1993-05-28
ATE66694T1 (de) 1991-09-15
MY102936A (en) 1993-03-31
HUT40810A (en) 1987-02-27
DE3681058D1 (de) 1991-10-02
GR860874B (en) 1986-07-30
DK152286A (da) 1986-10-04
AU5378686A (en) 1986-10-09
SU1604144A3 (ru) 1990-10-30
NO170421C (no) 1992-10-14
CS223386A2 (en) 1988-02-15
US4857317A (en) 1989-08-15
NO170421B (no) 1992-07-06
US4683294A (en) 1987-07-28
PH22110A (en) 1988-06-01
EG17972A (en) 1991-06-30
EP0199698B1 (fr) 1991-08-28
NO861234L (no) 1986-10-06
NZ215618A (en) 1988-08-30
IE58854B1 (en) 1993-11-17
PL152568B1 (en) 1991-01-31
KR860008276A (ko) 1986-11-14
DK152286D0 (da) 1986-04-03
EP0199698A2 (fr) 1986-10-29

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Date Code Title Description
FD1A Patent lapsed

Effective date: 19970203