KR920012425A - 동물세포 배양용 고농도 배지 및 배양공정 - Google Patents

동물세포 배양용 고농도 배지 및 배양공정 Download PDF

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KR920012425A
KR920012425A KR1019900022184A KR900022184A KR920012425A KR 920012425 A KR920012425 A KR 920012425A KR 1019900022184 A KR1019900022184 A KR 1019900022184A KR 900022184 A KR900022184 A KR 900022184A KR 920012425 A KR920012425 A KR 920012425A
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medium
high concentration
concentration
culture
cells
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KR1019900022184A
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KR930006117B1 (ko
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조의철
김동일
백승복
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허영섭
재단법인 목암생명공학연구소
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Priority to KR1019900022184A priority Critical patent/KR930006117B1/ko
Priority to JP3045623A priority patent/JPH0739372A/ja
Priority to GB919105264A priority patent/GB9105264D0/en
Priority to ITMI910718A priority patent/IT1245235B/it
Priority to CH1361/91A priority patent/CH685706A5/de
Priority to DE4115029A priority patent/DE4115029A1/de
Priority to FR9107977A priority patent/FR2671098B1/fr
Priority to GB9116321A priority patent/GB2251249B/en
Publication of KR920012425A publication Critical patent/KR920012425A/ko
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Publication of KR930006117B1 publication Critical patent/KR930006117B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
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  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

내용 없음

Description

동불세포 배양용 고농도 배지 및 배양공정
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제5는 고농도 내지 MBRI 40-20와 40-03을 이용한 유가식 배양공정중 잔존 포도당 종도의 곡선도 및 그것을 기준으로 한 유가식 고농도 배지의 참가방식을 나타낸 공정도이다. 제6도는 고농도 배지 MBRI 40-20와 40-03을 이용한 유가식 배양공정중 세표융합주의 세포증식 곡선도이다. 제7도는 고농도 배지 MBRI 40-20와 40-03을 이용한 세표융합주의 유가식 배양공정중 세포융합주에 의해 생성되는 간염표면 항원에 대한 단일세표군 항체의 생성곡선도이다.

Claims (4)

  1. 비부착성 포유동물세포의 체의배양에 있어서, 종래의 RPMI 1640배지중 각 영양성분을 강화한 고농도 배지군 및 그 배지군을 이용한 고농도 현탁배양 공정.
  2. 제1항에 있어서, 종래의 RPMI 1640배지에서의 각 성분간의 몰농도비율, 각 배지성분의 혈중농도, 각 영양성분이나 무기염들간의 비율, 배지의 삼투압 등의 인자들을 고려하여 배지의 아미노산류, 비타민류 및 기타 영양성분을 각각 RPMI 1640배지에서의 2.5배, 5배, 10배, 50배로 강화한 고농도 배지.
  3. 제1항에 있어서, 마우스 유래의 세포융합주, 사람의 임파종세포, 사람의 골수종 세포의 비부착성 포유동물 세포의 고농도 배양에 사용할 수 있는 것을 특징으로 하는 고농도 배지 및 그 농도 현탁배양 공정.
  4. 제1항에 있어서, 고농도 배지군을 이용하여, 배지중 잔존 포도당 농도를 기준으로, 전체의 10%내외의 고농도 배지를 첨가하여 2,500시간동안 고농도 배양을 가능케 하고, 단일세포군 항체등 포유동물 세포가 생산하는 생리활성 물질을 고농도 연속 생산하는 것을 특징으로 하는 유가식 배양방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900022184A 1990-12-28 1990-12-28 동물세포 배양용 고농도 배지 및 배양공정 KR930006117B1 (ko)

Priority Applications (8)

Application Number Priority Date Filing Date Title
KR1019900022184A KR930006117B1 (ko) 1990-12-28 1990-12-28 동물세포 배양용 고농도 배지 및 배양공정
JP3045623A JPH0739372A (ja) 1990-12-28 1991-02-20 動物細胞培養用高濃度培地及び培養方法
GB919105264A GB9105264D0 (en) 1990-12-28 1991-03-13 High-density medium for animal cell culture
ITMI910718A IT1245235B (it) 1990-12-28 1991-03-18 Mezzo ad alta densita' per la coltura di cellule animali
CH1361/91A CH685706A5 (de) 1990-12-28 1991-05-07 Medium mit hoher Dichte für tierische und menschliche Zellkulturen.
DE4115029A DE4115029A1 (de) 1990-12-28 1991-05-08 Hochdichtes, hochkonzentriertes medium fuer die zuechtung von tierischen zellen
FR9107977A FR2671098B1 (fr) 1990-12-28 1991-06-27 Milieu haute densite pour culture de cellules animales.
GB9116321A GB2251249B (en) 1990-12-28 1991-07-29 High-density medium for animal cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900022184A KR930006117B1 (ko) 1990-12-28 1990-12-28 동물세포 배양용 고농도 배지 및 배양공정

Publications (2)

Publication Number Publication Date
KR920012425A true KR920012425A (ko) 1992-07-27
KR930006117B1 KR930006117B1 (ko) 1993-07-07

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KR1019900022184A KR930006117B1 (ko) 1990-12-28 1990-12-28 동물세포 배양용 고농도 배지 및 배양공정

Country Status (7)

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JP (1) JPH0739372A (ko)
KR (1) KR930006117B1 (ko)
CH (1) CH685706A5 (ko)
DE (1) DE4115029A1 (ko)
FR (1) FR2671098B1 (ko)
GB (1) GB9105264D0 (ko)
IT (1) IT1245235B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100481208B1 (ko) * 2002-09-27 2005-04-08 재단법인 목암생명공학연구소 세포 배양용 무혈청 배지

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020022458A (ko) * 2000-09-20 2002-03-27 복성해 수정란의 체외배양법
WO2006044594A2 (en) * 2004-10-15 2006-04-27 Monell Chemical Senses Center Methods for culturing mammalian taste cells
KR101399595B1 (ko) * 2012-05-29 2014-05-27 삼육대학교산학협력단 분갈이용 그물망

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media
JPS6251983A (ja) * 1985-09-02 1987-03-06 Hagiwara Yoshihide ヒト／ヒト・ハイブリド−マ培養用無血清培地
CS262822B1 (en) * 1986-10-03 1989-04-14 Kovar Jan Synthetic medium for the cultivation of myelomic cells
JPH03180175A (ja) * 1989-12-07 1991-08-06 Snow Brand Milk Prod Co Ltd 無血清培地

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100481208B1 (ko) * 2002-09-27 2005-04-08 재단법인 목암생명공학연구소 세포 배양용 무혈청 배지

Also Published As

Publication number Publication date
DE4115029C2 (ko) 1993-06-09
FR2671098A1 (fr) 1992-07-03
KR930006117B1 (ko) 1993-07-07
IT1245235B (it) 1994-09-13
DE4115029A1 (de) 1992-07-02
ITMI910718A1 (it) 1992-09-18
GB9105264D0 (en) 1991-04-24
JPH0739372A (ja) 1995-02-10
CH685706A5 (de) 1995-09-15
FR2671098B1 (fr) 1994-12-30
ITMI910718A0 (it) 1991-03-18

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