JPS6480286A - Method for purifying enzyme - Google Patents

Method for purifying enzyme

Info

Publication number
JPS6480286A
JPS6480286A JP23635087A JP23635087A JPS6480286A JP S6480286 A JPS6480286 A JP S6480286A JP 23635087 A JP23635087 A JP 23635087A JP 23635087 A JP23635087 A JP 23635087A JP S6480286 A JPS6480286 A JP S6480286A
Authority
JP
Japan
Prior art keywords
lipase
sephadex
column chromatography
gel
cylindraceae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23635087A
Other languages
Japanese (ja)
Inventor
Masahide Nakada
Tadashi Funada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NOF Corp
Original Assignee
Nippon Oil and Fats Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Oil and Fats Co Ltd filed Critical Nippon Oil and Fats Co Ltd
Priority to JP23635087A priority Critical patent/JPS6480286A/en
Publication of JPS6480286A publication Critical patent/JPS6480286A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To carry out fractionation into 1,3-diglyceride lipase and 2- monoglyceride lipase, by subjecting a lipase obtained by cultivating Candida.cylindraceae to treatment using column chromatography. CONSTITUTION:An ion exchange gel, such as DEAE-Sephadex(R) or SP- Sephadex(R), or gel for gel filtration, such as Sephadex(R) G-150 or G-100, or both are used as a carrier, treated with MacIlvaine buffer solution (0.01mol. citric acid and 0.02mol. disodium phosphate; pH 3.8), etc., filled in a column having 1-10cm diameter, 50-200cm length, etc., and used for column chromatography. A lipase obtained by cultivating Candida.cylindraceae, such as lipase OF, is treated by the above-mentioned column chromatography and fractionated into 1,3-diglyceride lipase and 2-monoglyceride lipase.
JP23635087A 1987-09-22 1987-09-22 Method for purifying enzyme Pending JPS6480286A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23635087A JPS6480286A (en) 1987-09-22 1987-09-22 Method for purifying enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23635087A JPS6480286A (en) 1987-09-22 1987-09-22 Method for purifying enzyme

Publications (1)

Publication Number Publication Date
JPS6480286A true JPS6480286A (en) 1989-03-27

Family

ID=16999505

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23635087A Pending JPS6480286A (en) 1987-09-22 1987-09-22 Method for purifying enzyme

Country Status (1)

Country Link
JP (1) JPS6480286A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100592871B1 (en) * 2004-12-23 2006-06-26 재단법인 포항산업과학연구원 Apparutus for fixing replacement of sheet for tailer welding blank
JP2016037556A (en) * 2014-08-07 2016-03-22 横浜ゴム株式会社 Rubber composition and pneumatic tire

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100592871B1 (en) * 2004-12-23 2006-06-26 재단법인 포항산업과학연구원 Apparutus for fixing replacement of sheet for tailer welding blank
JP2016037556A (en) * 2014-08-07 2016-03-22 横浜ゴム株式会社 Rubber composition and pneumatic tire

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