JPS6480286A - Method for purifying enzyme - Google Patents
Method for purifying enzymeInfo
- Publication number
- JPS6480286A JPS6480286A JP23635087A JP23635087A JPS6480286A JP S6480286 A JPS6480286 A JP S6480286A JP 23635087 A JP23635087 A JP 23635087A JP 23635087 A JP23635087 A JP 23635087A JP S6480286 A JPS6480286 A JP S6480286A
- Authority
- JP
- Japan
- Prior art keywords
- lipase
- sephadex
- column chromatography
- gel
- cylindraceae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To carry out fractionation into 1,3-diglyceride lipase and 2- monoglyceride lipase, by subjecting a lipase obtained by cultivating Candida.cylindraceae to treatment using column chromatography. CONSTITUTION:An ion exchange gel, such as DEAE-Sephadex(R) or SP- Sephadex(R), or gel for gel filtration, such as Sephadex(R) G-150 or G-100, or both are used as a carrier, treated with MacIlvaine buffer solution (0.01mol. citric acid and 0.02mol. disodium phosphate; pH 3.8), etc., filled in a column having 1-10cm diameter, 50-200cm length, etc., and used for column chromatography. A lipase obtained by cultivating Candida.cylindraceae, such as lipase OF, is treated by the above-mentioned column chromatography and fractionated into 1,3-diglyceride lipase and 2-monoglyceride lipase.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23635087A JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23635087A JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6480286A true JPS6480286A (en) | 1989-03-27 |
Family
ID=16999505
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP23635087A Pending JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6480286A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100592871B1 (en) * | 2004-12-23 | 2006-06-26 | 재단법인 포항산업과학연구원 | Apparutus for fixing replacement of sheet for tailer welding blank |
JP2016037556A (en) * | 2014-08-07 | 2016-03-22 | 横浜ゴム株式会社 | Rubber composition and pneumatic tire |
-
1987
- 1987-09-22 JP JP23635087A patent/JPS6480286A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100592871B1 (en) * | 2004-12-23 | 2006-06-26 | 재단법인 포항산업과학연구원 | Apparutus for fixing replacement of sheet for tailer welding blank |
JP2016037556A (en) * | 2014-08-07 | 2016-03-22 | 横浜ゴム株式会社 | Rubber composition and pneumatic tire |
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