WO2023204396A1 - 펩신 소화율이 우수한 고단백 미세조류 바이오매스, 배양 방법 및 이의 용도 - Google Patents
펩신 소화율이 우수한 고단백 미세조류 바이오매스, 배양 방법 및 이의 용도 Download PDFInfo
- Publication number
- WO2023204396A1 WO2023204396A1 PCT/KR2023/001183 KR2023001183W WO2023204396A1 WO 2023204396 A1 WO2023204396 A1 WO 2023204396A1 KR 2023001183 W KR2023001183 W KR 2023001183W WO 2023204396 A1 WO2023204396 A1 WO 2023204396A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biomass
- microalgae
- weight
- schizochytrium
- genus
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 title claims abstract description 104
- 235000019621 digestibility Nutrition 0.000 title claims abstract description 46
- 102000057297 Pepsin A Human genes 0.000 title claims abstract description 45
- 108090000284 Pepsin A Proteins 0.000 title claims abstract description 45
- 229940111202 pepsin Drugs 0.000 title claims abstract description 45
- 238000012136 culture method Methods 0.000 title claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 39
- 235000020660 omega-3 fatty acid Nutrition 0.000 claims abstract description 13
- 229940012843 omega-3 fatty acid Drugs 0.000 claims abstract description 12
- 239000006014 omega-3 oil Substances 0.000 claims abstract description 6
- 241000233671 Schizochytrium Species 0.000 claims description 53
- 239000001963 growth medium Substances 0.000 claims description 32
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 29
- 150000001413 amino acids Chemical class 0.000 claims description 29
- 235000019784 crude fat Nutrition 0.000 claims description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 229910052799 carbon Inorganic materials 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 22
- 239000002609 medium Substances 0.000 claims description 20
- 235000019750 Crude protein Nutrition 0.000 claims description 19
- 229910052757 nitrogen Inorganic materials 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 12
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 claims description 11
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 claims description 9
- 235000020673 eicosapentaenoic acid Nutrition 0.000 claims description 9
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 claims description 9
- 229960005135 eicosapentaenoic acid Drugs 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 235000020669 docosahexaenoic acid Nutrition 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 5
- 229940090949 docosahexaenoic acid Drugs 0.000 claims description 5
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 235000010344 sodium nitrate Nutrition 0.000 claims description 2
- 239000004317 sodium nitrate Substances 0.000 claims description 2
- 235000019733 Fish meal Nutrition 0.000 abstract description 10
- 241001465754 Metazoa Species 0.000 abstract description 10
- 239000004467 fishmeal Substances 0.000 abstract description 10
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 8
- 229930195729 fatty acid Natural products 0.000 abstract description 8
- 239000000194 fatty acid Substances 0.000 abstract description 8
- 150000004665 fatty acids Chemical class 0.000 abstract description 7
- 238000002156 mixing Methods 0.000 abstract description 6
- 230000000052 comparative effect Effects 0.000 description 22
- 238000004458 analytical method Methods 0.000 description 19
- 235000018102 proteins Nutrition 0.000 description 13
- 102000004169 proteins and genes Human genes 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 241000251468 Actinopterygii Species 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- CYQFCXCEBYINGO-IAGOWNOFSA-N delta1-THC Chemical compound C1=C(C)CC[C@H]2C(C)(C)OC3=CC(CCCCC)=CC(O)=C3[C@@H]21 CYQFCXCEBYINGO-IAGOWNOFSA-N 0.000 description 6
- 241001467333 Thraustochytriaceae Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 241000286209 Phasianidae Species 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 239000003674 animal food additive Substances 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 239000002577 cryoprotective agent Substances 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 235000011121 sodium hydroxide Nutrition 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000238424 Crustacea Species 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000598397 Schizochytrium sp. Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 235000021120 animal protein Nutrition 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 235000008504 concentrate Nutrition 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 239000005996 Blood meal Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000238586 Cirripedia Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229920000426 Microplastic Polymers 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 230000035425 carbon utilization Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- -1 fatty acid calcium salts Chemical class 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012729 immediate-release (IR) formulation Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- JBXYCUKPDAAYAS-UHFFFAOYSA-N methanol;trifluoroborane Chemical compound OC.FB(F)F JBXYCUKPDAAYAS-UHFFFAOYSA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 238000009372 pisciculture Methods 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 235000019624 protein content Nutrition 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046001 vitamin b complex Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
Definitions
- the present invention relates to high-protein microalgae biomass with excellent pepsin digestibility, cultivation method, and use thereof.
- Fishmeal is a protein source that is widely used not only in fish feed but also in feed for various livestock species.
- environmental pollution problems such as fish catch regulations, heavy metals, and microplastics have recently been raised, the development of new protein sources that can replace fishmeal is required.
- Korean Patent No. 1564829 discloses a mixed feed composition for fish farming containing microalgae by-products.
- the present inventors developed a new protein source containing omega-3 fatty acid, a useful fatty acid produced through fermentation of microalgae of the Thraustochytrid series. Furthermore, by finding a composition with excellent pepsin digestibility, we present a new sustainable and environmentally friendly protein source.
- An example of the present application provides biomass derived from microalgae of the genus Schizochytrium , which has excellent pepsin digestibility and contains more than 46% of total amino acids relative to the dry weight of biomass.
- Another example of the present application provides a feed composition containing biomass derived from microalgae of the Schizochytrium genus.
- Another example of the present application provides a food composition containing biomass derived from microalgae of the Schizochytrium genus.
- Another example of the present application is culturing microalgae of the genus Schizochytrium under conditions in which the concentration of residual carbon source in the culture medium is maintained at 6% or less compared to the total culture medium; It provides a method of cultivating microalgae of the genus Schizochytrium, including.
- An example of the present application provides biomass derived from microalgae of the genus Schizochytrium , which has excellent pepsin digestibility and contains more than 46% by weight of total amino acids relative to the dry weight of biomass.
- Thraustochytrid refers to microalgae of the order Thraustochytriales.
- Schizochytrium sp used in this specification is one of the genus names belonging to the family Thraustochytriaceae of the order Traustochytriales, and the term “genus Schizochytrium (genus Schizochytrium ) )” can be used interchangeably.
- microalgae refers to small algae that cannot be seen with the naked eye and can only be seen through a microscope, and refers to organisms that live freely floating in water. There are various types of microalgae, including strains that cannot photosynthesize and grow only heterotrophically.
- the microalgae of the Schizochytrium genus may be the Schizochytrium genus CD01-5004 strain deposited under the accession number KCTC14345BP, but is not limited thereto.
- biomass used in this specification refers to living organisms such as plants, animals, and microorganisms that can be used as chemical energy, that is, an energy source of bioenergy, and refers to a specific energy source that exists ecologically within unit time and space. It also refers to the weight or amount of energy of a living organism.
- the biomass includes, but is not limited to, compounds secreted by cells, and may contain cells and/or intracellular contents as well as extracellular materials.
- the biomass may be the microalgae of the Schizochytrium genus itself, its culture, its dried product, its crushed product, or a product produced by cultivating or fermenting the microalgae, or a concentrate of the biomass or It may be a dry product, but is not limited thereto.
- the “culture” of microalgae of the Schizochytrium genus refers to a product produced by culturing the microalgae, and may specifically be a culture medium containing microalgae or a culture filtrate from which microalgae have been removed from the culture medium. It is not limited to this.
- the “dried material” of the Schizochytrium genus microalgae culture is one in which moisture has been removed from the microalgae culture, and may be, for example, the form of dried cells of the microalgae, but is not limited thereto.
- the “shredded material” of the dried product refers to a general term for the result of crushing the dried material from which moisture has been removed from the microalgae culture, and may be, for example, dried bacterial cell powder, but is not limited thereto.
- the culture of the microalgae of the Schizochytrium genus can be prepared by inoculating the microalgae into a microalgae culture medium and using a culture method known in the art.
- the dried product of the culture and its lysate are also known in the art. It can be manufactured according to the processing or drying method of microalgae or culture medium.
- the biomass derived from microalgae of the Schizochytrium genus is 46% by weight, 48% by weight, 49% by weight, 50% by weight, or 52% by weight, 46 to 60% by weight, based on the dry weight of biomass.
- the biomass derived from microalgae of the Schizochytrium genus is at least 60% by weight, at least 64% by weight, at least 67% by weight, or at least 70% by weight, 60 to 85% by weight, and 60 to 80% by weight, based on the dry weight of the biomass.
- It may contain from 70% by weight or 69 to 70% by weight of crude protein, and the upper limit may be 85% by weight or less, 80% by weight or less, and 78% by weight or less, but is not limited thereto.
- the biomass derived from microalgae of the Schizochytrium genus is 5% by weight or more, 7% by weight or more, 9% by weight or more, 10% by weight or more, 14% by weight or more, 20% by weight or more, 1 to 1% by weight, based on the dry weight of biomass. 20% by weight, 4 to 23% by weight, 9 to 25% by weight, 9 to 23% by weight, 5 to 18% by weight, 10 to 18% by weight, 10 to 15% by weight, 12 to 18% by weight, 12 to 15% by weight % or 13 to 15% by weight of crude fat.
- crude protein may be used interchangeably with “crude protein,” which measures total nitrogen in general analysis of food and feed, and adds 6.25 (the reciprocal of the average nitrogen content of protein, 1/0.16). This refers to the protein obtained by multiplying .
- crude fat refers to a component extracted with ether using a Soxhlet extractor in general analysis of food and feed
- crude fat content refers to “crude fat amount” or “total lipid” or “total It can be used interchangeably with “fatty acid.”
- DHA docosahexaenoic acid
- ALA alpha-linolenic acid
- EPA eicosapentaenoic acid
- cervonic acid cervonic acid, and it can also be abbreviated as 22:6 n-3.
- EPA eicosapentaenoic acid
- EPA is one of the polyunsaturated fatty acids with the chemical formula of C 20 H 30 O 2 and corresponds to an omega-3 fatty acid along with ALA and DHA. It can also be abbreviated as 20:5 n-3.
- the biomass derived from microalgae of the Schizochytrium genus is 39% by weight or more, 40% by weight or more, 41% by weight or more, 45% by weight or more, 49% by weight or more, 50% by weight or more, 39 to 60% by weight, based on the total crude fat content.
- Weight %, 39 to 55% by weight, 39 to 50% by weight, 42 to 55% by weight, 42 to 50% by weight, 43 to 55% by weight, 43 to 50% by weight, 45 to 50% by weight, 47 to 50% by weight Alternatively, it may contain 49 to 50% by weight of docosahexaenoic acid (DHA).
- DHA docosahexaenoic acid
- the biomass derived from microalgae of the Schizochytrium genus is 0.5% by weight or more, 1.0% by weight or more, 1.5% by weight or more, 2.0% by weight or more, 2.1% by weight or more, 3.5% by weight or more, 0.5% by weight, based on the total crude fat content.
- EPA eicosapentaenoic acid
- the biomass derived from microalgae of the Schizochytrium genus is 41% by weight, 42% by weight, 50% by weight, 42 to 55% by weight, 42 to 52% by weight, 43 to 55% by weight, based on the total crude fat content. It may contain 43 to 52% by weight, 49 to 55% by weight, or 49 to 52% by weight of omega-3 fatty acids.
- the biomass derived from microalgae of the Schizochytrium genus has a pepsin digestibility of 68.7% or more, 69% or more, 70% or more, 73% or more, 75% or more, 77% or more, 80% or more, 83% or more, 89% or more, 69% to 95%, 69% to 90%, 70% to 95%, 70% to 90%, 73% to 95%, or 73% to 90%, 75% to 95%, 75% to 90%, 85 % to 95%, 85% to 90%, 87% to 95%, 87% to 90%, 89% to 95% or 89% to 90%.
- pepsin digestibility used in this specification is used to analyze the quality of animal protein raw materials, and refers to an indicator that can compare how much digestion has occurred in a certain environment using pepsin extracted from the stomach of an animal.
- the biomass may be cultured by a method for cultivating microalgae of the Schizochytrium genus according to one aspect.
- compositions containing biomass derived from microalgae of the genus Schizochytrium which has excellent pepsin digestibility and contains more than 46% by weight of total amino acids relative to the dry weight of the biomass.
- the composition may be characterized by excellent pepsin digestibility and can be used in the production of food and/or feed.
- Another aspect of the present application provides a feed composition containing biomass derived from microalgae of the genus Schizochytrium , which has excellent pepsin digestibility and contains more than 46% by weight of total amino acids relative to the dry weight of the biomass.
- the biomass derived from microalgae of the Schizochytrium genus is as described above.
- feed composition refers to the feed fed to animals.
- the feed composition refers to a substance that supplies organic or inorganic nutrients necessary to maintain animal life or produce meat, milk, etc.
- the feed composition may additionally contain nutrients necessary for maintaining animal life or producing meat, milk, etc.
- the feed composition can be manufactured from various types of feed known in the art, and may specifically include concentrate feed, roughage feed, and/or special feed.
- feed additive is added to feed for the purpose of various effects such as supplementing nutrients and preventing weight loss, improving digestibility of fiber in feed, improving milk quality, preventing reproductive disorders and improving conception rate, and preventing high temperature stress in the summer.
- the feed additive of this application corresponds to supplementary feed under the Feed Management Act, and includes mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and complex minerals, mineral preparations such as trace minerals such as zinc, copper, cobalt, and selenium, and kerotene.
- vitamins such as vitamin E, vitamins A, D, E, nicotinic acid, and vitamin B complex
- protective amino acids such as methionine and lysic acid
- protective fatty acids such as fatty acid calcium salts
- probiotics lactic acid bacteria
- yeast cultures and molds.
- Live bacteria such as fermented products, yeast agents, etc. may be additionally included.
- Another aspect of the present application provides a food composition containing biomass derived from microalgae of the genus Schizochytrium , which has excellent pepsin digestibility and contains more than 46% by weight of total amino acids relative to the dry weight of the biomass.
- the biomass derived from microalgae of the Schizochytrium genus is as described above.
- food composition includes all forms of functional food, nutritional supplements, health food, and food additives, and includes all forms of the above types. Food compositions can be manufactured in various forms according to conventional methods known in the art.
- Compositions of the present application may include grains such as ground or ground wheat, oats, barley, corn and rice; Vegetable protein feeds, such as those based on soybean and sunflower; Animal protein feeds such as blood meal, meat meal, bone meal and fish meal; It may further include dry ingredients such as sugar and dairy products, such as various powdered milk and whey powder, and may further include nutritional supplements, digestion and absorption enhancers, growth promoters, etc.
- composition of the present application may be administered to animals alone or in combination with other feed additives in an edible carrier. Additionally, the composition can be easily administered to animals as a top dressing, by mixing them directly into feed, or in an oral formulation separate from feed. When the composition is administered separately from feed, it can be prepared as an immediate-release or sustained-release formulation by combining it with a pharmaceutically acceptable edible carrier, as is well known in the art.
- a pharmaceutically acceptable edible carrier can be solid or liquid, such as corn starch, lactose, sucrose, soybean flakes, peanut oil, olive oil, sesame oil and propylene glycol.
- a solid carrier the composition may be a tablet, capsule, powder, troche or sugar-containing tablet, or a top dressing in microdisperse form.
- a liquid carrier the composition may be in the form of a gelatin soft capsule, or a syrup, suspension, emulsion, or solution.
- composition of the present application may contain, for example, preservatives, stabilizers, wetting or emulsifying agents, cryoprotectants, or excipients.
- cryoprotectant may be one or more selected from the group consisting of glycerol, trehalose, maltodextrin, skim milk powder, and starch.
- the preservative, stabilizer, or excipient may be included in the composition in an effective amount sufficient to reduce deterioration of microalgae of the genus Schizochytrium included in the composition.
- the cryoprotectant may be included in the composition in an effective amount sufficient to reduce the degradation of microalgae of the genus Schizochytrium included in the composition when the composition is in a dried state.
- the composition can be used by injecting, spraying, or mixing it and adding it to animal feed.
- compositions of the present application can be applied to the diet of a number of animals, including, but not limited to, mammals, birds, fish, crustaceans, cephalopods, reptiles and amphibians.
- the mammals may include pigs, cows, sheep, goats, laboratory rodents, or pets, and the birds may include poultry, and the poultry may include chickens, turkeys, ducks, geese, It may include, but is not limited to, pheasant or quail.
- the fish may include commercial farmed fish, their fry, ornamental fish, etc.
- the crustaceans may include shrimp, barnacles, etc., but are not limited thereto.
- the composition can also be applied to the diet of rotifers, which are zooplankton.
- Another aspect of the present application includes culturing microalgae of the genus Schizochytrium under conditions in which the residual carbon source concentration in the culture medium is maintained at 6% or less compared to the total culture medium; It provides a method of cultivating microalgae of the genus Schizochytrium, including.
- the microalgae of the Schizochytrium genus may be the Schizochytrium genus CD01-5004 strain deposited under the accession number KCTC14345BP, but is not limited thereto.
- the biomass derived from microalgae of the Schizochytrium genus is as described above.
- culture means growing the microalgae under appropriately controlled environmental conditions.
- the culture process of the present application can be carried out according to appropriate media and culture conditions known in the art. This culture process can be easily adjusted and used by those skilled in the art depending on the microalgae selected.
- culture medium may refer to a medium for culturing microalgae, and may mean that the medium for culturing microalgae further includes microalgae and/or products produced by microalgae. .
- the culture conditions are specifically, the residual carbon source concentration in the culture medium is maintained at 6% or less, 5% or less, 4% or less, 3% or less, or 2% or less compared to the total culture medium. It can be performed under certain conditions.
- the cultivation of microalgae of the Schizochytrium genus of the present application may be performed under heterotrophic conditions, but is not limited thereto.
- heterotrophic material used in this specification is a nutritional method that relies on organic matter obtained from outside the body as an energy or nutrient source, and is a term corresponding to autotrophy, and can be used interchangeably with the term 'cancer culture'.
- the step of cultivating microalgae of the Schizochytrium genus is not particularly limited thereto, but may be performed by known batch culture methods, continuous culture methods, fed-batch culture methods, etc.
- the medium and other culture conditions used for cultivating microalgae in the present application can be any medium used for cultivating conventional microalgae without any particular restrictions.
- the microalgae of the present application can be cultured under aerobic conditions in a typical medium containing an appropriate carbon source, nitrogen source, phosphorus, inorganic compound, amino acid and/or vitamin, etc. while controlling temperature, pH, etc.
- the appropriate pH (e.g., pH 5 to 9, specifically pH 5 to 8) can be adjusted using a basic compound (e.g., sodium hydroxide, potassium hydroxide, or ammonia) or an acidic compound (e.g., phosphoric acid or sulfuric acid).
- a basic compound e.g., sodium hydroxide, potassium hydroxide, or ammonia
- an acidic compound e.g., phosphoric acid or sulfuric acid
- the medium used in the step of cultivating microalgae of the Schizochytrium genus of the present application may be MJW01 medium, but is not limited thereto.
- the carbon source contained in the medium used in the step of culturing microalgae of the Schizochytrium genus is any selected from the group consisting of glucose, fructose, maltose, galactose, mannose, sucrose, arabinose, xylose and glycerol. It may be one or more, and specifically may be one or more selected from the group consisting of glucose, fructose and sucrose, but is not limited thereto.
- Nitrogen sources included in the medium used in the step of cultivating microalgae of the Schizochytrium genus include urea, ammonia water, ammonium sulfate (NH 4 ) 2 SO 4 ), yeast extract, peptone, It may include one or more species selected from the group consisting of sodium nitrate ( NaNO 3 ), but is not limited thereto as long as it is a nitrogen source used for cultivating microalgae.
- the medium used in the step of cultivating microalgae of the Schizochytrium genus may include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, and corresponding sodium-containing salts individually or in a mixture as a phosphorus source. Not limited.
- the medium used in the step of cultivating microalgae of the Schizochytrium genus includes 0.01 to 5 g/L of yeast extract, 1.0 to 3.0 g/L of KH 2 PO 4 , and 5 to 20 g/L of K 2 HPO 4 may, but is not limited to this.
- the culture temperature can be maintained at 20 to 45°C or 25 to 40°C, and culture can be performed for about 10 to 70 hours, but is not limited thereto.
- the method of culturing microalgae of the Schizochytrium genus is to supply the concentration of residual carbon source in the culture medium to be maintained at a maximum of 6% or less compared to the total culture medium, thereby increasing the total amino acid content compared to the dry weight of microalgae biomass, thereby increasing pepsin digestibility. there is.
- the present invention developed an alternative protein source that can replace fishmeal through a microalgae-based fermentation process.
- the composition presented in the present invention not only has a high protein content, but also contains omega-3 fatty acids, which are useful fatty acids similar to fish meal, so it can directly replace fish meal when mixing feed.
- the high-protein microalgae biomass presented in the present invention shows a high pepsin digestibility of more than 70% as a result of analysis by pepsin digestibility analysis, so even if the mixing ratio in the feed increases, it does not negatively affect the overall digestibility, and microalgae Since it is a protein source produced based on protein, there is no problem with environmental pollution, and it has the advantage of being able to produce stably by minimizing the influence of the external environment.
- the strain was grown in sterilized MJW01 medium (Glucose 30 g/L, MgSO 4 ⁇ 7H 2 O 3.0 g/L, Na 2 SO 4 10 g/L, NaCl 1.0 g/L, yeast extract 9.0 g/L, MSG ⁇ 1H 2 O 1.0 g/L, NaNO 3 1.0 g/L, KH 2 PO 4 0.1 g/L, K 2 HPO 4 0.5 g/L, CaCl 2 0.5 g/L, vitamin mixed solution 10 ml/L) It was cultured in a 250 mL flask at 26°C and 200 rpm for 30 hours.
- Comparative Example 1 Preparation of biomass by culturing the strain under conditions of 7-9% (w/v ratio) of carbon source concentration compared to the culture medium.
- the seed cultured strain was cultured in sterilized MJW01 medium (Glucose 30 g/L, MgSO 4 ⁇ 7H 2 O 3.0 g/L, Na 2 SO 4 10 g/L, NaCl 1.0 g/L, yeast extract 0.5 g/L, MSG ⁇ 1H 2 O 1.0 g/L, NaNO 3 5.0 g/L, KH 2 PO 4 1.5 g/L, K 2 HPO 4 10 g/L, CaCl 2 0.5 g/L, vitamin mixed solution 10 ml/L) was dispensed into a 5L fermenter and cultured at 28°C, 300 rpm, 0.6 vvm, and pH 6.8.
- MJW01 medium Glucose 30 g/L, MgSO 4 ⁇ 7H 2 O 3.0 g/L, Na 2 SO 4 10 g/L, NaCl 1.0 g/L, yeast extract 0.5 g/L, MSG ⁇ 1H 2 O 1.0 g/
- the residual carbon source concentration in the culture medium was supplied to be maintained in the range of 7-9% (w/v ratio) compared to the total culture medium.
- the culture was continued by supplying a nitrogen source from immediately after the start of the culture until the end of the culture. In order to maintain the appropriate nitrogen source concentration, the nitrogen source was supplied to the culture medium by turning it on and off repeatedly. Glucose was used as the carbon source, and ammonium sulfate was used as the nitrogen source.
- the bacterial culture medium was freeze-dried or drum-dried to obtain dry biomass of Comparative Example 1, which was a control group.
- Example 1 Production of biomass by culturing the strain under conditions of 0 to 2% (w/v ratio) of carbon source concentration compared to the culture medium.
- the culture conditions of Comparative Example 1 While the culture continued (45 hours) while maintaining the composition of the MJW01 medium, the concentration of the residual carbon source in the culture medium was in the range of 0 to 2% (w/v ratio) compared to the total culture medium. It was supplied to be maintained in . The remaining culture conditions were cultured in the same manner as in Comparative Example 1 to secure the dry biomass of Example 1.
- Example 2 Production of biomass by culturing the strain under conditions of up to 2-5% carbon source concentration (w/v ratio) compared to the culture medium.
- Example 3 Production of biomass in which the strain was cultured under conditions where the carbon source concentration was up to 4-7% (w/v ratio) compared to the culture medium.
- the concentration of the residual carbon source in the culture medium was 4-7% (w/v ratio) compared to the total culture medium. It was supplied to be maintained within the range.
- an 8.3 M hydrochloric acid solution was added to 2 g of dried microalgae cells of Comparative Example 1 and Examples 1 to 3, and a hydrolysis reaction was performed at 80°C. Then, 30 mL of ethyl ether and 20 mL of petroleum ether were added, mixed for 30 seconds, and the process of centrifugation was repeated three or more times. The separated solvent layer was transferred to a round flask whose weight was previously measured, and then placed in a container from which the solvent and remaining moisture were removed through nitrogen purging and dried.
- the total oil content was calculated by measuring the weight of the remaining oil after drying the solvent, and the omega-3 (DHA and EPA) content contained in the oil was calculated using methanolic 0.5N NaOH and 14% trifluoroborane methanol (BF 3 ). After pretreatment, it was measured using gas chromatography.
- each dried microalgae biomass sample obtained in Comparative Example 1 and Examples 1 to 3 was placed in an analysis tube using a Kjeldahl system (Kjeltec 2100), sulfur phase and catalyst were added and decomposed in a decomposer, The digestion tube was mounted on an autosampler.
- the crude protein content of dried microalgae biomass is measured by cooling and collecting the gaseous ammonia generated by heating and distilling with caustic soda and steam in the Kjeldahl apparatus, then titrating it with hydrochloric acid and automatically calculating the nitrogen content. did.
- Example 1 70.50% 45.75% 8.50% 46.25% 1.77% 48.02%
- Example 1 64.10% 48.10% 22.80% 40.51% 1.86% 42.37%
- Example 2 69.90% 52.78% 14.40% 49.40% 2.11% 51.51%
- Example 3 77.20% 52.18% 12.20% 42.47% 1.06% 43.53%
- Example 1 maintained the concentration of the residual carbon source in the culture medium in a low range of 0 to 2% during the cultivation process, thereby increasing microalgae biomass. Although I was able to increase my total amino acid content, I confirmed that my crude fat content was also increasing.
- Example 2 the concentration range of the carbon source remaining in the culture medium while the culture was in progress was adjusted to 2-5%.
- the concentration of the carbon source was maintained in the range of 2 to 5% in Example 2
- the crude protein content was maintained at the level of 70%
- the crude fat content was similar to that of Comparative Example 1 and Example 2. It was confirmed that it appeared in the middle range of Example 1.
- the total amino acid content in biomass was found to be 52.78%, the highest value among culture conditions.
- Example 3 the concentration of carbon source remaining in the culture medium was maintained in the range of 4 to 7% while the culture was in progress.
- the concentration of the carbon source was maintained in the range of 4 to 7%, the crude protein content was 77.20%, the total amino acid content was 52.18%, and the crude fat content was 12.20%, showing the highest crude protein content among the culture conditions.
- the Kjeldahl nitrogen content in Calculation Formula 1 was obtained by proceeding with the method described in the crude protein analysis using the Kjeldahl automatic analyzer in Experimental Example 1-3.
- the pepsin digestibility of the dried microalgae biomass of Comparative Example 1 was found to be relatively low at 68.6%.
- the pepsin digestibility of the dried microalgae biomass of Example 1 was 73.40%, and the pepsin digestibility of the dried microalgae biomass of Example 2 was 89.62%. It was confirmed that the pepsin digestibility of the dried microalgae biomass in 3 was 83.20% and that it exhibited a high pepsin digestibility.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
Description
조단백
/biomass |
총아미노산
/biomass |
조지방
/biomass |
DHA
/조지방 |
EPA
/조지방 |
오메가-3 지방산
/조지방 |
|
비교예 1 | 70.50% | 45.75% | 8.50% | 46.25% | 1.77% | 48.02% |
실시예 1 | 64.10% | 48.10% | 22.80% | 40.51% | 1.86% | 42.37% |
실시예 2 | 69.90% | 52.78% | 14.40% | 49.40% | 2.11% | 51.51% |
실시예 3 | 77.20% | 52.18% | 12.20% | 42.47% | 1.06% | 43.53% |
펩신소화율 | |
비교예 1 | 68.59% |
실시예 1 | 73.40% |
실시예 2 | 89.62% |
실시예 3 | 83.2% |
Claims (19)
- 스키조키트리움 (Schizochytrium) 속 미세조류를 배양액 내의 잔류 탄소원 농도가 총 배양액 대비 7% 이하에서 유지되는 조건에서 배양하는 단계; 를 포함하는, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 스키조키트리움 (Schizochytrium) 속 미세조류는 기탁번호 KCTC14345BP로 기탁된 스키조키트리움 (Schizochytrium) 속 CD01-5004 균주인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 배양하는 단계는 MJW01 배지에서 수행하는 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 배지의 탄소원은 글루코오스 (glucose), 프럭토오스 (fructose) 및 수크로오스 (sucrose)로 이루어지는 군에서 선택되는 1종 이상인 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 배지는 질소원으로 요소 (urea), 암모니아수, 황산 암모늄 (ammonium sulfate), 효모 추출물, 펩톤 (peptone) 및 질산 나트륨 (sodium nitrate)으로 이루어지는 군에서 선택되는 1종 이상을 포함하는 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 배지는 효모 추출물 0.01 내지 5 g/L, KH2PO4 1.0 내지 3.0 g/L, K2HPO4 5 내지 20 g/L를 포함하는 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 배양은 10 내지 70 시간 동안 수행되는 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 미세조류 배양방법은 미세조류 건조 중량 대비 총 아미노산 함량을 증가시켜 펩신 소화율을 높이는 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항에 있어서, 상기 펩신 소화율은 70% 이상인 것인, 스키조키트리움 속 미세조류 유래 바이오매스 제조방법.
- 제1항의 제조방법으로 제조된 펩신 소화율이 우수한 스키조키트리움 (Schizochytrium) 속 미세조류 유래 바이오매스.
- 제10항에 있어서, 상기 스키조키트리움 (Schizochytrium) 속 미세조류는 기탁번호 KCTC14345BP로 기탁된 스키조키트리움(Schizochytrium) 속 CD01-5004 균주인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 바이오매스 건조 중량 대비 60 중량% 이상의 조단백을 함유하는 것인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 바이오매스 건조 중량 대비 4 중량% 내지 23 중량%의 조지방을 함유하는 것인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 총 조지방 중량 대비 40 중량% 이상의 도코사헥사엔산 (docosahexaenoic acid)을 함유하는 것인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 총 조지방 중량 대비 0.5 중량% 이상의 에이코사펜타엔산 (eicosapentaenoic acid)을 함유하는 것인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 총 조지방 중량 대비 41 중량% 이상의 오메가-3 지방산을 함유하는 것인, 바이오매스.
- 제10항에 있어서, 상기 바이오매스는 펩신 소화율이 70% 이상인, 바이오매스.
- 제10항의 스키조키트리움 속 미세조류 유래 바이오매스를 포함하는 사료 조성물.
- 제10항의 스키조키트리움 속 미세조류 유래 바이오매스를 포함하는 식품 조성물.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2023256399A AU2023256399A1 (en) | 2022-04-18 | 2023-01-26 | High-protein microalgal biomass having excellent pepsin digestibility, culture method and use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2022-0047721 | 2022-04-18 | ||
KR1020220047721A KR20230148659A (ko) | 2022-04-18 | 2022-04-18 | 펩신 소화율이 우수한 고단백 미세조류 바이오매스, 배양 방법 및 이의 용도 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2023204396A1 true WO2023204396A1 (ko) | 2023-10-26 |
Family
ID=88420238
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2023/001183 WO2023204396A1 (ko) | 2022-04-18 | 2023-01-26 | 펩신 소화율이 우수한 고단백 미세조류 바이오매스, 배양 방법 및 이의 용도 |
Country Status (4)
Country | Link |
---|---|
KR (1) | KR20230148659A (ko) |
AU (1) | AU2023256399A1 (ko) |
TW (1) | TW202342729A (ko) |
WO (1) | WO2023204396A1 (ko) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110037588A (ko) * | 2009-10-07 | 2011-04-13 | 대상 주식회사 | 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법 |
KR101564829B1 (ko) | 2014-02-05 | 2015-10-30 | 강릉원주대학교산학협력단 | 미세조류 부산물을 함유하는 어류 양식용 배합사료조성물 |
US20160298149A1 (en) * | 2013-12-19 | 2016-10-13 | Roquette Freres | Method for enriching the biomass of thraustochytrium genus microalgae with dha |
KR20180029249A (ko) * | 2015-07-17 | 2018-03-20 | 페르망탈그 | 트라우스토키트리드의 단백질이 풍부한 바이오매스, 배양 방법 및 용도 |
KR101847551B1 (ko) * | 2016-11-04 | 2018-04-10 | 전북대학교산학협력단 | 카로티노이드 계열의 항산화 색소 및 dha를 포함한 바이오오일을 고생산하는 돌연변이 미세조류인 스키조키트리움 속 shg104 균주 및 이의 용도 |
KR20200074047A (ko) * | 2018-12-13 | 2020-06-24 | 파이코일바이오텍코리아 (주) | Epa, dha 및 아라키돈산 고함량 기능성 유지 생산 방법 |
-
2022
- 2022-04-18 KR KR1020220047721A patent/KR20230148659A/ko unknown
-
2023
- 2023-01-26 AU AU2023256399A patent/AU2023256399A1/en active Pending
- 2023-01-26 WO PCT/KR2023/001183 patent/WO2023204396A1/ko active Application Filing
- 2023-02-06 TW TW112104141A patent/TW202342729A/zh unknown
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110037588A (ko) * | 2009-10-07 | 2011-04-13 | 대상 주식회사 | 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법 |
US20160298149A1 (en) * | 2013-12-19 | 2016-10-13 | Roquette Freres | Method for enriching the biomass of thraustochytrium genus microalgae with dha |
KR101564829B1 (ko) | 2014-02-05 | 2015-10-30 | 강릉원주대학교산학협력단 | 미세조류 부산물을 함유하는 어류 양식용 배합사료조성물 |
KR20180029249A (ko) * | 2015-07-17 | 2018-03-20 | 페르망탈그 | 트라우스토키트리드의 단백질이 풍부한 바이오매스, 배양 방법 및 용도 |
KR101847551B1 (ko) * | 2016-11-04 | 2018-04-10 | 전북대학교산학협력단 | 카로티노이드 계열의 항산화 색소 및 dha를 포함한 바이오오일을 고생산하는 돌연변이 미세조류인 스키조키트리움 속 shg104 균주 및 이의 용도 |
KR20200074047A (ko) * | 2018-12-13 | 2020-06-24 | 파이코일바이오텍코리아 (주) | Epa, dha 및 아라키돈산 고함량 기능성 유지 생산 방법 |
Also Published As
Publication number | Publication date |
---|---|
TW202342729A (zh) | 2023-11-01 |
AU2023256399A1 (en) | 2024-06-13 |
KR20230148659A (ko) | 2023-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2022124590A1 (ko) | 신규한 스키조키트리움 속 균주 및 이를 이용한 다중불포화지방산 생산방법 | |
HU229062B1 (hu) | Eljárás dokozahexaénsav és dokozapentaénsav elõállítására | |
JP2019524049A (ja) | バチルスサブチリスとバチルスリケニフォルミスを含む飼料添加剤、前記添加剤を含む飼料組成物及び前記飼料添加剤の製造方法 | |
WO2020179999A1 (ko) | 바실러스 서브틸리스 cjbs303 및 이를 포함하는 조성물 | |
JPH0511956B2 (ko) | ||
WO2018160000A1 (ko) | 발효 구아밀 제조방법 | |
WO2023204396A1 (ko) | 펩신 소화율이 우수한 고단백 미세조류 바이오매스, 배양 방법 및 이의 용도 | |
WO2022124482A1 (ko) | 단일 미세조류로부터 단백질 및 오메가-3 지방산을 포함하는 바이오매스를 제조하는 방법 및 이에 의해 제조된 바이오매스 | |
WO2023080400A1 (ko) | 세포 내 오일 함유량이 높은 신규한 스키조키트리움 속 균주 및 이를 이용한 오메가3를 함유한 오일 생산방법 | |
WO2024039163A1 (ko) | 고함량의 단백질, 항산화 색소 및 오메가-3 지방산을 포함하는 바이오매스를생산하는 신규한 스키조키트리움 속 균주 및 이의 용도 | |
EP0071970B1 (en) | Novel antibiotic 76-11, process for the production thereof, anticoccidiosis agent and domestic animals growth accelerator comprising the same as an effective ingredient | |
FR2543409A1 (fr) | Additif alimentaire et aliment pour animaux contenant bacillus licheniformis et nouvelle souche de ce micro-organisme | |
WO2023080399A1 (ko) | 세포 내 오일 추출이 용이한 신규한 스키조키트리움 속 균주 및 이를 이용한 오메가3를 함유한 오일 생산방법 | |
WO2024128494A1 (ko) | 신규한 스키조키트리움 속 균주 및 이를 이용한 오메가-3를 함유한 오일 생산방법 | |
CA3241365A1 (en) | High-protein microalgal biomass having excellent pepsin digestibility, culture method and use thereof | |
JP2004147620A (ja) | 栄養強化飼料 | |
TWI844072B (zh) | 具有高細胞內油含量的新穎裂殖壺菌屬物種(Schizochytrium sp.)品系及使用其生產含ω-3之油的方法 | |
JPH08242774A (ja) | 産卵鶏用色調改善飼料 | |
KR101890530B1 (ko) | 폐기줄풀로부터 셀룰로오스, 단백질 및 지질을 분해하는 미생물군의 분리와 줄풀을 이용한 사료첨가제를 생산하는 방법 | |
KR20240094204A (ko) | 신규한 스키조키트리움 속 균주 및 이를 이용한 오메가-3를 함유한 오일 생산방법 | |
JPH03228652A (ja) | 稚魚用飼料としての生物の培養方法 | |
HU190814B (en) | Process for preparing new polyether type antibiotics | |
CN1007267B (zh) | 抗生素6270的制备方法 | |
CN1014673B (zh) | 抗生素6270及其盐类作为一种抗球虫病剂和饲料添加剂的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23791980 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2023256399 Country of ref document: AU Ref document number: AU2023256399 Country of ref document: AU |
|
ENP | Entry into the national phase |
Ref document number: 2023256399 Country of ref document: AU Date of ref document: 20230126 Kind code of ref document: A |