WO2023120560A1 - Boisson aromatisée à la bière - Google Patents

Boisson aromatisée à la bière Download PDF

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Publication number
WO2023120560A1
WO2023120560A1 PCT/JP2022/047034 JP2022047034W WO2023120560A1 WO 2023120560 A1 WO2023120560 A1 WO 2023120560A1 JP 2022047034 W JP2022047034 W JP 2022047034W WO 2023120560 A1 WO2023120560 A1 WO 2023120560A1
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Prior art keywords
beer
taste beverage
less
taste
beverage
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PCT/JP2022/047034
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English (en)
Japanese (ja)
Inventor
智恵 小西
悠一 加藤
太一 丸橋
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サントリーホールディングス株式会社
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/56Flavouring or bittering agents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00Fermentation processes for beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • C12C5/02Additives for beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/04Preparation or treatment of the mash
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/20Boiling the beerwort
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/26Cooling beerwort; Clarifying beerwort during or after the cooling
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/04Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/04Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • C12G3/06Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with flavouring ingredients

Definitions

  • the present invention relates to a beer-taste beverage and a method for producing a beer-taste beverage.
  • Patent Document 1 0.3 to 5 ppm of quasine and/or 0.5 to 5 ppm of quinine are included for the purpose of providing a beer-taste beverage having beer-like bitterness and aftertaste without using hops.
  • An invention relating to a beer-taste beverage is described.
  • the present invention provides a beer-taste beverage containing linalool at a predetermined content or less, with the alcohol content and turbidity adjusted to within predetermined ranges.
  • the present invention provides the following aspects [1] to [13].
  • alcohol content is 5.0 (v / v)% or less
  • Turbidity is 22 Helm or more
  • a method for producing a beer-taste beverage according to any one of [1] to [11] above, comprising the following steps (1) to (2), and having a pore size of less than 30 ⁇ m after step (2) A method for producing a beer-taste beverage without filtration through a filter.
  • - Step (1) Using various raw materials, performing at least one of saccharification treatment, boiling treatment, and solid content removal treatment to obtain a pre-fermentation liquid.
  • - Process (2) The process of adding yeast to the said pre-fermentation liquid, and fermenting.
  • - Process (3) A process of adjusting the turbidity of the beer-taste beverage after the process (2).
  • the beer-taste beverage of one preferred embodiment of the present invention has, for example, a texture suitable for beer-taste beverages, and can become a novel beverage that is more like beer-taste beverages.
  • a beer-taste beverage according to a more preferred embodiment of the present invention has a texture and refreshing feeling suitable for a beer-taste beverage, and can be a novel beverage that is more like a beer-taste beverage.
  • a beer-taste beverage according to a more preferred embodiment of the present invention has a chewy texture and crispness suitable for beer-taste beverages, and, for example, has unsuitable yeast odor and cosmetic aroma even as a fermented beer-taste beverage using yeast. is suppressed, and a new beverage that is more like a beer-taste beverage can be obtained.
  • the upper and lower limits can be combined arbitrarily.
  • the numerical range is described as “preferably 3.0 to 15, more preferably 3.2 to 13”
  • the range of "3.0 to 13" or “3.2 to 15” Ranges are also included in the numerical ranges recited herein.
  • the numerical range is described as "preferably 30 or more, more preferably 40 or more, and preferably 100 or less, more preferably 80 or less”
  • “30 to 80” Ranges and ranges from “40 to 100” are also included in the numerical ranges described herein.
  • the description of "60 to 100” means a range of "60 or more and 100 or less”.
  • Beer-taste beverage refers to an alcohol-containing or non-alcoholic carbonated beverage having a beer-like flavor. Therefore, “beer-taste beverages” include not only beer and fermented beer-taste beverages, which are fermented malt beverages obtained by fermenting malt, hops, and water using yeast as raw materials, but also esters and high-grade beverages. It also includes carbonated beverages to which beer flavors such as alcohol and lactone are added.
  • Beer flavors include isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate, ethyl caprylate, isoamyl propionate, linalool, geraniol, citral, 4-vinylguaiacol (4-VG).
  • the beer-taste beverage of one embodiment of the present invention may be a fermented beer-taste beverage that has undergone a fermentation process using yeast, or may be a non-fermented beer-taste beverage that has not undergone a fermentation process.
  • the fermented beer-taste beverage may be an ale beer-taste beverage brewed through a fermentation process using top-fermenting yeast (Saccharomyces, etc.), or an ale beer-taste beverage brewed through a fermentation process using bottom-fermenting yeast (Saccharomyces, etc.). It may also be a lager beer-taste beverage, a pilsner beer-taste beverage, or the like.
  • "fermentation” as used herein may be alcoholic fermentation in which alcohol is produced, or non-alcoholic fermentation in which alcohol is not produced.
  • beer-taste beverage of one embodiment of the present invention may be a malt-using beer-taste beverage that uses malt as a raw material, or a malt-free beer-taste beverage that does not use malt.
  • a beer-taste beverage is preferable, and a beer-taste beverage using barley malt is more preferable.
  • the beer-taste beverage of one embodiment of the present invention has an alcohol content (ethanol content) of 5.0 (v/v)% or less.
  • the beer-taste beverage of one embodiment of the present invention may be an alcohol-containing beer-taste beverage having an alcohol content of 1 (v/v)% or more, or a non-alcoholic beverage having an alcohol content of less than 1 (v/v)%. It may be a beer-taste beverage.
  • the non-alcoholic beer-taste beverage may be a non-alcoholic fermented beer-taste beverage produced by removing the alcohol produced in the fermentation process after the fermentation process, and a beer-like beverage without the fermentation process. It may be a non-alcoholic, non-fermented beer-taste beverage prepared to have a flavor. In addition, it may include those that undergo a non-alcoholic fermentation process that does not produce alcohol.
  • the beer-taste beverage of one embodiment of the present invention is adjusted to have an alcohol content of 5.0 (v/v)% or less, it can be a beer-taste beverage that is moderately intoxicating and is resistant to alcoholic beverages. It can be a drink suitable for sensitive consumers. From the above viewpoint, the alcohol content of the beer-taste beverage of one embodiment of the present invention is 4.8 (v/v)% or less, 4.6 (v/v)% or less, or 4.4 (v/v)% or less.
  • the alcohol content of the beer-taste beverage of one embodiment of the present invention is 0.1 (v/v)% or more and 0.3 (v/v). % or more, 0.5 (v/v)% or more, 0.7 (v/v)% or more, 1.0 (v/v)% or more, 1.2 (v/v)% or more, 1.4 (v/v)% or more, 1.5 (v/v)% or more, 1.6 (v/v)% or more, 1.8 (v/v)% or more, 2.0 (v/v)% 2.2 (v/v)% or more, 2.4 (v/v)% or more, 2.5 (v/v)% or more, 2.6 (v/v)% or more, 2.8 ( v/v)% or more, 3.0 (v/v)% or more, 3.2 (v/v)% or more, or 3.4 (v/v)% or more.
  • the alcohol content is indicated by volume/volume-based percentage ((v/v)%).
  • the alcohol content of the beverage can be measured by any known method, and for example, it can be measured by a vibrating density meter.
  • the alcohol content of the beer-taste beverage of one embodiment of the present invention includes the type of raw material (wheat, malt, corn grits, sugar solution, etc.), the amount of raw material, the type of enzyme, the amount of enzyme added, the timing of enzyme addition, Saccharification time in the brewing tank, protein decomposition time in the brewing tank, pH in the brewing process (from malt input to wort production process before adding yeast), amount of acid used for pH adjustment, pH adjustment Timing (at the time of preparation, at the time of fermentation, at the end of fermentation, before beer filtration, after beer filtration, etc.), set temperature and holding time for each temperature range when preparing wort (including during saccharification), original extract of pre-fermentation liquid Concentration, original extract concentration in the fermentation process, fermentation conditions (oxygen concentration, aeration conditions, yeast variety, amount of yeast added, yeast growth number, timing of yeast removal, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) , Whether or not to add spirits, brewed alcohol,
  • the beer-taste beverage of one embodiment of the present invention may contain grain-derived spirits (distilled liquor) as an alcohol component in order to adjust the alcohol content to fall within the above range.
  • spirits refer to grains such as wheat, rice, buckwheat, corn, potato, and sugarcane as raw materials, saccharified using malt or, if necessary, an enzyme agent, fermented using yeast, and then distilled.
  • alcoholic beverages obtained by Grain which is a raw material for spirits, is preferably a plant belonging to the family Poaceae, and more preferably wheat.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage that does not contain spirits, from the viewpoint of producing a beer-taste beverage that has a good taste and is like beer.
  • the beer-taste beverage of one embodiment of the present invention is adjusted to have an alcohol content of 5.0 (v/v)% or less from the viewpoint of making it a beer-taste beverage that is moderately intoxicating.
  • the texture suitable for beer-taste beverages tends to decrease. Therefore, the beer-taste beverage of one embodiment of the present invention is a beverage that easily gives a moderate feeling of intoxication and that imparts an appropriate texture to the beer-taste beverage.
  • Beer-taste beverages with high turbidity are rich in umami components such as amino acids and high-molecular proteins, as well as vitamins, minerals, high-molecular carbohydrates and yeast. It is thought that the beverage will spread a gorgeous and fruity aroma, and will give a sufficiently satisfying feeling to the drink that is suitable for beer-taste beverages.
  • the turbidity of the beer-taste beverage of one embodiment of the present invention is 22 Helm or more, preferably 30 Helm or more, more preferably 40 Helm or more, and more preferably 50 Helm, from the viewpoint of making the beer-taste beverage imparted with an appropriate texture. More preferably 70 Helm or more, still more preferably 100 Helm or more, still more preferably 200 Helm or more, still more preferably 300 Helm or more, still more preferably 500 Helm or more, still more preferably 700 Helm or more, still more preferably 850 Helm or more, particularly preferably It is 1000 Helm or more, and may be 1100 Helm or more, 1200 Helm or more, 1300 Helm or more, 1400 Helm or more, or 1500 Helm or more.
  • 9000 Helm or less 8000 Helm or less, 7000 Helm or less, 6000 Helm or less, 5500 Helm or less, 5000 Helm or less, 4500 Helm or less, 4000 Helm or less, 3500 Helm or less, 3000 Helm or less, 2500 Helm or less, 2300 Helm or less, 2100 Helm or less, or 20 It may be 00 Helm or less.
  • the ratio of the turbidity (unit: Helm) and the alcohol content (unit: (v/v)%) [turbidity/alcohol content] is From the viewpoint of making it easy to drink and making a beer-taste beverage with improved texture, it is preferably 15 or more, more preferably 20 or more, more preferably 25 or more, more preferably 30 or more, still more preferably 50 or more, still more preferably is 100 or more, more preferably 150 or more, still more preferably 200 or more, even more preferably 250 or more, particularly preferably 300 or more, and may be 350 or more, 400 or more, 410 or more, or 420 or more, In addition, from the viewpoint of making a beer-taste beverage that more effectively suppresses inappropriate offensive odors, it is preferably 1500 or less, more preferably 1450 or less, more preferably 1400 or less, more preferably 1350 or less, still more preferably 1300 or less, and even more preferably 1300 or less.
  • preferably 1200 or less, more preferably 1100 or less, still more preferably 1000 or less, even more preferably 900 or less, particularly preferably 800 or less, furthermore 750 or less, 700 or less, 650 or less, 600 or less, or 550 or less may be
  • turbidity can be measured by the method described in Analytica EBC, Method 9.29, Haze in beer. Specifically, the sample to be measured is placed in a constant temperature water bath at 0°C and held for 48 hours, lightly stirred to homogenize the sample, and placed again in a constant temperature water bath at 0°C and held for several minutes until bubbles disappear. The total turbidity can then be measured by reading the 90° scattered light value as "turbidity".
  • the turbidity of the beer-taste beverage of one aspect of the present invention can be adjusted, for example, by not performing filtration through a filter of less than 30 ⁇ m after the step of fermentation using yeast. By not filtering through the filter, the yeast-containing beverage is obtained.
  • the beer-taste beverage of one aspect of the present invention may be a fermented beer-taste beverage obtained without filtration through a filter with a pore size of less than 30 ⁇ m after the fermentation step using yeast.
  • the turbidity of the resulting beer-taste beverage can be adjusted to be high by heat-treating the saccharified liquid before the fermentation step by gradually raising the temperature.
  • the fermented liquid obtained after the fermentation process using yeast is centrifuged under appropriate conditions. is also adjustable. It can also be adjusted by adjusting various conditions (aging period, aging temperature, etc.) of the aging treatment for aging the fermented liquid, or by filtering a part of the fermented liquid through a filter with a pore size of 30 ⁇ m or more.
  • the number of yeasts is preferably 6 cells/cells from the viewpoint of producing a beer-taste beverage with turbidity adjusted to the above range and an appropriate texture.
  • 100 mL or more more preferably 1.0 ⁇ 10 2 cells/100 mL or more, more preferably 1.0 ⁇ 10 3 cells/100 mL or more, still more preferably 1.0 ⁇ 10 4 cells/100 mL or more, still more preferably 1.0 ⁇ 10 4 cells/100 mL or more.
  • the "yeast count" can be measured by the method described in Analytica EBC, Method 3.1.1.1, Haemocytometry.
  • the beverage may be filtered through a filter with a pore size of less than 30 ⁇ m after the fermentation process using yeast. It is preferred that the beverage is not filtered through a filter. That is, one aspect of the present invention may be an unfiltered beer-taste beverage. Moreover, the beer-taste beverage of one embodiment of the present invention may be, for example, a beverage that is filtered through a filter with a pore size of 30 ⁇ m or more after a fermentation step using yeast.
  • the beer-taste beverage of one aspect of the present invention has a predetermined pore size (for example, a pore size of 30 ⁇ m) in order to remove raw material residue such as malt used as a raw material when preparing a saccharified solution before fermentation. (above) is not limited to filtering through the filter.
  • the turbidity of the non-fermented beer-taste beverage can be adjusted, for example, by not filtering through a filter with a pore size of less than 30 ⁇ m in the manufacturing process. It can be adjusted, and can also be adjusted by appropriately setting the manufacturing conditions described in the manufacturing method described later.
  • a filter with a predetermined pore size for example, pore size of 30 ⁇ m or more
  • filtration through a filter with a predetermined pore size for example, pore size of 30 ⁇ m or more
  • beer-taste beverages with high turbidity have problems such as offensive odors unsuitable for beer-taste beverages and a lack of refreshment when drunk.
  • the beverage when the beverage is a fermented beer-taste beverage, yeast odor tends to occur, and when it is a non-fermented beer-taste beverage, an offensive odor derived from the amount of raw materials may occur. Therefore, the beer-taste beverage of one embodiment of the present invention contains linalool to mask unfavorable offensive odors peculiar to beer-taste beverages, such as yeast odors and offensive odors derived from raw materials, making it difficult to perceive such offensive odors. adjusting.
  • the beer-taste beverage of one embodiment of the present invention has a linalool content of 120 ⁇ g/L or less. .
  • the content of linalool is 120 ⁇ g/L or less, preferably 115 ⁇ g/L, from the viewpoint of producing a beer-taste beverage that effectively suppresses inappropriate cosmetic aromas.
  • the beer-taste beverage of one embodiment of the present invention from the viewpoint of making it a beer-taste beverage that can more effectively suppress unsuitable offensive odors (fermented odors, offensive odors derived from raw materials, etc.), it is preferably 0.01 ⁇ g / L or more, more preferably 0.05 ⁇ g/L or more, more preferably 0.07 ⁇ g/L or more, still more preferably 0.10 ⁇ g/L or more, still more preferably 0.30 ⁇ g/L or more, still more preferably 0.50 ⁇ g/L or more, Especially preferably 1.0 ⁇ g/L or more, further 1.5 ⁇ g/L or more, 2.0 ⁇ g/L or more, 2.5 ⁇ g/L or more, 3.0 ⁇ g/L or more, 3.5 ⁇ g/L or more, 4 0 ⁇ g/L or more, 4.5 ⁇ g/L or more, 5.0 ⁇ g/L or more, 5.5 ⁇ g/L or more, 6.0 ⁇ g/L or more
  • the ratio of the linalool content (unit: ⁇ g/L) to the turbidity (unit: Helm) [linalool content/turbidity] is an undesirable offensive odor
  • preferably 2.0 or less more preferably 1.0 or less, still more preferably 0.50 or less, still more preferably 0.10 or less, even more preferably 0.050 or less, still more preferably 0.030 or less , particularly preferably 0.010 or less.
  • the content of linalool is determined by the gas chromatography described in J. Agric. Food Chem., 2013, 61 (47), pp 11303-1131 (Characterization of the Key Aroma Compounds in Two Bavarian Wheat Beers by Means of the Sensomics Approach). It can be measured using a tograph mass spectrometer (GC-MS).
  • GC-MS tograph mass spectrometer
  • the content of linalool can be adjusted, for example, by adjusting the amount of linalool added, the variety of raw materials with a high linalool content (for example, hops, etc.) and the amount used, and It can be controlled by adjusting the timing of addition of the raw material.
  • the sugar concentration of the beer-taste beverage of one aspect of the present invention is, for example, more than 0.1 g/100 mL, more than 0.5 g/100 mL, more than 1.0 g/100 mL, more than 1.5 g/100 mL, and 1.6 g/100 mL.
  • sucrose is based on the Nutrition Labeling Standards for Foods (Ministry of Health, Labor and Welfare Notification No. 176 of 2003, Partially Revised Consumer Affairs Agency Notification No. 8 of September 27, 2013). It refers to carbohydrates, and specifically, it means a target food from which protein, lipid, dietary fiber, ash, alcohol and water have been removed. Therefore, the amount of carbohydrates in a food can be calculated by subtracting the amount of protein, lipid, dietary fiber, ash and water from the weight of the food.
  • the amounts of protein, lipid, dietary fiber, ash and moisture can be measured by the methods listed in the Nutrition Labeling Standards.
  • the amount of protein was measured by the nitrogen quantitative conversion method
  • the amount of lipid was measured by the ether extraction method
  • the amount of dietary fiber was measured by the Prosky method
  • the amount of ash was measured by the direct incineration method.
  • the amount of water can be measured by the reduced pressure heat drying method.
  • the sugar concentration of the beer-taste beverage of one aspect of the present invention depends on the type of enzyme, the amount added, the timing of addition, the set temperature and holding time of each temperature range when preparing the saccharified solution, and various factors in the fermentation process. It can be adjusted by appropriately setting the conditions (amount and timing of addition of yeast, fermentation temperature, fermentation time) and the like.
  • the total polyphenol content is preferably 10 mg/L or more, more preferably 30 mg/L or more, and more preferably 40 mg, from the viewpoint of making the beer-taste beverage imparted with an appropriate crispness.
  • /L or more more preferably 50 mg/L or more, still more preferably 70 mg/L or more, even more preferably 100 mg/L or more, particularly preferably 120 mg/L or more, preferably 500 mg/L or more, more preferably 450 mg /L or less, more preferably 400 mg/L or less, more preferably 350 mg/L or less, still more preferably 320 mg/L or less, still more preferably 300 mg/L or less, even more preferably 270 mg/L or less, particularly preferably 250 mg/L or less. L or less.
  • the ratio of the total polyphenol amount (unit: mg/L) to the turbidity (unit: Helm) [total polyphenol amount/turbidity] further improved the crispness.
  • it is preferably 0.001 or more, more preferably 0.005 or more, more preferably 0.010 or more, more preferably 0.020 or more, still more preferably 0.030 or more, still more preferably 0.050 or more, more preferably 0.060 or more, particularly preferably 0.070 or more, and may be 0.080 or more, 0.090 or more, 0.10 or more, or 0.11 or more
  • it is preferably 10.0 or less, more preferably 9.0 or less, more preferably 8.0 or less, more preferably 7.0 or less, and even more preferably is 6.0 or less, more preferably 5.0 or less, even more preferably 3.0 or less, particularly preferably
  • total amount of polyphenols means the total amount of polyphenols contained in the total amount (100% by mass) of the beer-taste beverage.
  • Polyphenol means a compound in which two or more hydrogen atoms of an aromatic hydrocarbon are substituted with hydroxyl groups, and specifically includes flavonol, isoflavone, tannin, catechin, quercetin, anthocyanin, and the like.
  • the total polyphenol content is, for example, the method described in the revised BCOJ beer analysis method (published by the Brewing Association of Japan, Beer Brewers Association International Technical Committee [Analysis Committee] edited by 2013 supplementary revision). can be measured by
  • the total polyphenol content can be controlled by adjusting the amount of polyphenol-rich raw materials such as barley malt and malt husks.
  • polyphenol-rich raw materials such as barley malt and malt husks.
  • malt with husks has a high polyphenol content
  • soybean, yeast extract, wheat, wheat malt, etc. have a low polyphenol content.
  • the content of free amino acids is preferably 1.0 mg/100 mL or more, more preferably 2.0 mg/100 mL, from the viewpoint of making the beer-taste beverage with improved texture.
  • the content of free amino acids is the method described in "8.18 Free amino acid nitrogen (IM) ninhydrin colorimetric method" of the revised BCOJ beer analysis method (Beer Brewers Association International Technical Committee, 2013). can be measured in
  • the content of free amino acids is determined, for example, by the type of raw material (malt, corn grits, etc.), the type of enzyme, the amount of enzyme (including proteolytic enzyme) added, the timing of enzyme addition, and the proteolytic time in the preparation tank.
  • pH in the brewing tank set temperature and holding time for each temperature range when preparing wort, boiling time and pH in the boiling process, original extract concentration in the pre-fermentation liquid, original extract concentration in the fermentation process, fermentation Conditions (yeast variety, amount of yeast added, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, etc.) can be appropriately set and adjusted.
  • yeast variety amount of yeast added, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, etc.
  • the NIBEM-T value of the beer-taste beverage of one embodiment of the present invention is preferably 50 seconds or more, more preferably 100 seconds or more, and more preferably 120 seconds, from the viewpoint of producing a beer-taste beverage with improved foamability. 150 seconds or more, more preferably 180 seconds or more, still more preferably 210 seconds or more, still more preferably 230 seconds or more, and particularly preferably 240 seconds or more.
  • the NIBEM-T value is the type of raw material (malt, corn grits, etc.), the type of enzyme, the amount of enzyme (including proteolytic enzyme) added, the timing of enzyme addition, the protein decomposition time in the preparation tank, and the pH, set temperature and holding time for each temperature range when preparing wort, boiling time and pH in the boiling process, amount of hops added, original extract concentration in the pre-fermentation liquid, original extract concentration in the fermentation process, Fermentation conditions (yeast variety, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, etc.), addition and addition amount of raw materials that contribute to foaming, etc. should be appropriately set and adjusted. can be done.
  • the NIBEM-T value is "8.29" of the revised BCOJ beer analysis method (published by the Brewing Association of Japan, Beer Brewers Association International Technical Committee [Analysis Committee] edited in 2013). Foam can be measured by the method described in "Method for measuring foam retention using NIBEM-T".
  • the raw wort extract (O-Ex) concentration is preferably 6.0% by mass or more, more preferably 6.5% by mass or more, and more preferably 7.0% by mass. above, more preferably 7.5% by mass or more, still more preferably 8.0% by mass or more, even more preferably 8.5% by mass or more, particularly preferably 9.0% by mass or more, and preferably 20 0% by mass or less, more preferably 18.0% by mass or less, still more preferably 16.0% by mass or less, even more preferably 15.0% by mass or less, and particularly preferably 13.5% by mass or less.
  • the "original wort extract concentration" in this specification refers to the extract content under the Japanese Liquor Tax Law in alcohol-containing beverages with an alcohol content of 1 (v/v)% or more, that is, at a temperature of 15 ° C. It refers to the number of grams of non-volatile components contained in the original volume of 100 cm 3 .
  • the analysis method BOCJ beer analysis method (Japan Brewing Association fermentation, It refers to the extract value (% by mass) measured in accordance with Beer Brewers Association, 2013 revised edition)).
  • the malt ratio of the beer-taste beverage of one embodiment of the present invention is 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 55% by mass or more, 60% by mass or more, 65% by mass or more.
  • % by mass or more 66% by mass or more, 67% by mass or more, 70% by mass or more, 75% by mass or more, 80% by mass or more, 85% by mass or more, 90% by mass or more, 95% by mass or more, or 100% by mass 100% by mass or less, 98% by mass or less, 95% by mass or less, 90% by mass or less, 85% by mass or less, 80% by mass or less, 75% by mass or less, 70% by mass or less, 68% by mass or less , 67% by mass or less, or 66% by mass or less.
  • malt ratio means a value calculated in accordance with the Liquor Tax Act and liquor administration-related laws and regulations, which are enforced on April 1, 2018.
  • carbon source nitrogen source
  • examples of carbon sources that can be assimilated by yeast include monosaccharides, disaccharides, trisaccharides, sugar solutions thereof, and the like.
  • Nitrogen sources include yeast extract, soybean protein, malt, soybeans, yeast extract, peas, and wheat. Malt, ungerminated grains, decomposition products thereof, and the like.
  • ungerminated grains examples include ungerminated barley, wheat, rye, oats, oats, pigeons, oats, rice (white rice, brown rice, etc.), corn, korian, potato, beans (soybeans, peas, etc.), buckwheat, sorghum, millet, barnyard millet, and the like. Starches obtained from these grains and extracts thereof may also be used.
  • beer-taste beverage of one embodiment of the present invention may be beer.
  • beer refers to a beverage obtained by fermenting malt, hops, and water as raw materials using yeast. It means what is defined in the Liquor Tax Act and the Liquor Administration Related Laws and Regulations Interpretation Notice on the effective date. That is, when the beer-taste beverage of one embodiment of the present invention is beer, the alcohol content is adjusted by a fermentation process using yeast.
  • the color of the beer-taste beverage of one embodiment of the present invention is not particularly limited, but may be amber or golden like normal beer, black like dark beer, or colorless and transparent, or a coloring agent. etc. may be added to give the desired color.
  • the color of the beer-taste beverage can be determined with the naked eye, but may be defined by total light transmittance, chromaticity, or the like.
  • the pH of the beer-taste beverage of one embodiment of the present invention is 2.0 or higher, 2.2 or higher, 2.4 or higher, 2.6 or higher, 2.8 or higher, 3.0 or higher, 3.1 or higher, and 3.0 or higher. 2 or more, 3.3 or more, 3.4 or more, 3.5 or more, 3.6 or more, 3.7 or more, 3.8 or more, 3.9 or more, or 4.0 or more, preferably 5 .4 or less, 5.2 or less, 5.0 or less, 4.9 or less, 4.8 or less, 4.7 or less, 4.6 or less, or 4.5 or less.
  • the beer-taste beverage of one aspect of the present invention may be in any form as long as the beverage is packed in a container, and examples of the container include bottles, PET bottles, cans, and barrels, which are particularly easy to carry. cans, bottles, and PET bottles are preferable.
  • malt may be used together with water, or malt may not be used.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage using hops as a raw material, or may be a beverage using no hops.
  • preservatives, sweeteners, water-soluble dietary fibers, bittering agents or bitterness imparting agents, antioxidants, flavors, acidulants, salts, and the like may be used.
  • malt when malt is used as a cereal raw material other than malt , the malt refers to germinated seeds of barley, wheat, rye, oat, oat, pigeon, oat, etc. It refers to dried and rooted plants, and may be of any production area or variety.
  • Barley malt is preferable as the malt used in one aspect of the present invention. Barley malt is one of the most commonly used malts as a raw material for Japanese beer-taste beverages. There are two kinds of barley, such as two-rowed barley and six-rowed barley, and any of them may be used. In addition to normal malt, colored malt can also be used. When using colored malt, different types of colored malt may be appropriately combined and used, or one type of colored malt may be used.
  • grains other than malt with malt.
  • examples of such grains include wheat (barley, wheat, rye, oats, oats, pigeons, oats, etc.) that do not fall under malt, rice (polished rice, brown rice, etc.), corn, koryan, potato, etc. , beans (soybeans, peas, etc.), buckwheat, sorghum, millet, barley, and starches obtained therefrom, extracts thereof, and the like.
  • liquid sugar containing a carbon source when malt is not used, liquid sugar containing a carbon source, and beer-taste beverages using a nitrogen source as an amino acid-containing material (for example, soybean protein, etc.) such as the above-mentioned grains other than malt can be mentioned.
  • a nitrogen source as an amino acid-containing material (for example, soybean protein, etc.) such as the above-mentioned grains other than malt
  • Fruits, fruit skins, barks, leaves, flowers, stems, roots, and seeds of plants other than gramineous plants such as wheat that can be used as raw materials can be appropriately selected.
  • plants other than gramineous plants include citrus fruits, soft fruits, herbs, and spices.
  • citrus include orange, yuzu, lemon, lime, mandarin orange, grapefruit, Iyokan, kumquat, kabosu, bitter orange, shikuwasa, and sudachi.
  • Soft fruits include peaches, grapes, bananas, apples, grapes, pineapples, strawberries, pears, muscat grapes, and cassis.
  • Herbs and spices include coriander, pepper, fennel, Sichuan pepper, Japanese pepper, cardamom, caraway, nutmeg, mace, juniper berry, allspice, vanilla, elderberry, grains of paradise, anise, and star anise. mentioned. These may be used as they are, may be used after pulverization, may be used in the form of extracts extracted with an extraction solvent such as water or ethanol, or may be used in the form of squeezed juices (such as fruit juice). You may These may be used alone or in combination of two or more.
  • the above can be used as appropriate according to consumer tastes, but in order to enjoy the refreshing and refreshing taste of beer, the above citrus fruits, soft fruits, herbs, and spices should not be used as raw materials, or the amount used should be should be minimized.
  • cassis imparts an unsuitable milky flavor to beer, it is preferable not to use cassis or cassis juice as a raw material, or to minimize the amount used.
  • hops When hops are used in one aspect of the present invention, examples of the form of the hops include pellet hops, powdered hops, and hop extracts. As the hops to be used, processed hops such as isoformed hops and reduced hops may be used. When hops are used in one aspect of the present invention, the amount of hops added is appropriately adjusted, but is preferably 0.0001 to 1% by mass based on the total amount (100% by mass) of the ingredients of the beverage.
  • a beer-taste beverage using hops as a raw material is a beverage containing iso- ⁇ acids, which are components derived from hops.
  • the iso- ⁇ acid content of the beer-taste beverage using hops may be more than 0.1 ppm by mass, or more than 1.0 ppm by mass, based on the total amount (100% by mass) of the beer-taste beverage. There may be.
  • the iso- ⁇ acid content in the beer-taste beverage containing no hops may be 0.1 ppm by mass or less based on the total amount (100% by mass) of the beer-taste beverage.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage containing a preservative.
  • Preservatives used in one embodiment of the present invention include, for example, benzoic acid; benzoates such as sodium benzoate; benzoate esters such as propyl parahydroxybenzoate and butyl parahydroxybenzoate; and dimethyl dicarbonate.
  • a commercially available preparation such as Strong Samprezer (a mixture of sodium benzoate and butyl benzoate, manufactured by San-Eigen FFI Co., Ltd.) may be used. These preservatives may be used alone or in combination of two or more.
  • the amount of the preservative is preferably 5 to 1200 ppm by mass, more preferably 10 to 1100 ppm by mass, and even more preferably 15 to 1000 ppm by mass. , and more preferably 20 to 900 mass ppm.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage further containing a sweetener.
  • Sweeteners used in one embodiment of the present invention include commercially available saccharified solutions obtained by decomposing grain-derived starch with acid or enzymes, etc., commercially available sugars such as starch syrup, sucrose, trisaccharides or higher sugars, isomerized sugars, and sugars. Examples include alcohol, natural sweeteners such as stevia, and artificial sweeteners. These sweeteners may be used alone or in combination of two or more.
  • the form of these saccharides may be liquid such as solution, or solid such as powder.
  • starch raw material grains there are no particular restrictions on the type of starch raw material grains, the method of purifying starch, and the treatment conditions such as hydrolysis with enzymes or acids.
  • sugars with a higher proportion of maltose may be used by appropriately setting conditions for hydrolysis with an enzyme or acid.
  • sucrose, fructose, glucose, maltose, trehalose, maltotriose, maltotetraose, isomaltose, isomaltotriose, isomaltotetraose and their solutions (sugar solutions) can also be used.
  • artificial sweeteners include aspartame, acesulfame potassium (acesulfame K), sucralose, and neotame.
  • water-soluble dietary fibers examples include indigestible dextrin, polydextrose, guar gum decomposition products, pectin, glucomannan, alginic acid, laminarin, fucoidin, carrageenan, and the like, from the viewpoint of versatility such as stability and safety. , indigestible dextrin or polydextrose are preferred.
  • bitterness-imparting agents may further contain one or more selected from bittering agents and bitterness-imparting agents.
  • bitterness may be imparted by hops, and the following bittering agent or bitterness imparting agent may be used together with hops.
  • a bittering agent or a bitterness-imparting agent shown below may be used instead of hops.
  • the bittering agent or bitterness-imparting agent is not particularly limited, and those used as bitterness-imparting agents in ordinary beer and low-malt beer can be used.
  • bittering agents and bitterness-imparting agents may be used alone or in combination of two or more.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage further containing an antioxidant.
  • the antioxidant is not particularly limited, and those used as antioxidants in ordinary beer and low-malt beer can be used, and examples thereof include ascorbic acid, erythorbic acid, and catechin. These antioxidants may be used alone or in combination of two or more.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage further containing a flavor.
  • Flavors are not particularly limited, and common beer flavors can be used. Beer flavors are used for beer-like flavoring. Beer flavors include esters and higher alcohols, and specific examples include isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate, ethyl caprylate, isoamyl propionate, linalool, and geraniol.
  • the content of ester and higher alcohol flavorings depends on the addition of diluted water or carbonated water, the sugar composition and amino acid composition of the pre-fermentation liquid before adding yeast, the concentration of sugar and amino acids, and the original extract concentration of the pre-fermentation liquid. , Yeast variety, fermentation conditions (oxygen concentration, ventilation conditions, yeast variety, amount of yeast added, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), cooling timing, etc. You can set and adjust.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage further containing an acidulant.
  • the acidulant is not particularly limited as long as it is a substance having a sour taste. salt.
  • at least one selected from tartaric acid, phosphoric acid, citric acid, gluconic acid, lactic acid, malic acid, phytic acid, acetic acid, succinic acid and salts thereof is preferable, and tartaric acid, phosphoric acid, citric acid, lactic acid, At least one selected from tartaric acid, acetic acid and salts thereof is more preferred, and at least one selected from tartaric acid, phosphoric acid and lactic acid is even more preferred.
  • These acidulants may be used alone or in combination of two or more.
  • the beer-taste beverage of one embodiment of the present invention may be a beverage further containing salts.
  • salts include sodium chloride, acid potassium phosphate, acid calcium phosphate, ammonium phosphate, magnesium sulfate, calcium sulfate, potassium metabisulfite, calcium chloride, magnesium chloride, potassium nitrate, ammonium sulfate, potassium chloride, monosodium citrate, disodium citrate, trisodium citrate and the like. These salts may be used alone or in combination of two or more.
  • Carbon dioxide contained in the beer-taste beverage of one embodiment of the present invention may be carbon dioxide contained in raw materials, or may be dissolved by mixing with carbonated water or adding carbon dioxide.
  • the carbon dioxide gas generated in the fermentation process of the beer-taste beverage can be used as it is, but the amount of carbon dioxide gas may be adjusted by adding carbonated water as appropriate.
  • the carbon dioxide gas concentration of the beer-taste beverage of one embodiment of the present invention is preferably 0.30 (w/w)% or more, more preferably 0.35 (w/w)% or more, and more preferably 0.40 (w/w)% or more. /w)% or more, more preferably 0.42 (w/w)% or more, more preferably 0.45 (w/w)% or more, even more preferably 0.47 (w/w)% or more, Particularly preferably 0.50 (w/w)% or more, preferably 0.80 (w/w)% or less, more preferably 0.70 (w/w)% or less, and still more preferably 0.70 (w/w)% or less.
  • the carbon dioxide gas concentration is measured by immersing the container containing the target beverage in a water tank at 20°C for 30 minutes or longer while occasionally shaking the beverage to adjust the temperature of the beverage to 20°C, and then measuring the gas volume. It can be measured using an apparatus (eg, GVA-500 (manufactured by Kyoto Electronics Industry Co., Ltd.)).
  • the carbon dioxide pressure of the container-packed beverage may be appropriately adjusted within the range of the carbon dioxide concentration described above. It may be 4.5 kg/cm 2 or less, or 4.0 kg/cm 2 or less, or may be 0.20 kg/cm 2 or more, 0.50 kg/cm 2 or more, or 1.0 kg/cm 2 or more. Any combination of these upper limits and lower limits may be used . cm 2 or less, or 1.0 kg/cm 2 or more and 4.0 kg/cm 2 or less.
  • the gas pressure refers to the gas pressure within the container, except in special cases. The pressure is measured by a method well known to those skilled in the art.
  • additives may be added to the beer-taste beverage of one embodiment of the present invention, as necessary, as long as the effects of the present invention are not impaired.
  • additives include coloring agents, foaming agents, fermentation accelerators, yeast extracts, protein substances such as peptide-containing substances, and seasonings such as amino acids.
  • a coloring agent is used to impart a beer-like color to a beverage, and caramel coloring and the like can be used.
  • the foam-forming agent is used to form a beer-like foam in a beverage or to retain the foam of the beverage.
  • Proteins, peptide-containing materials such as collagen peptides, yeast extracts, and the like can be used as appropriate. Fermentation promoters are used to promote fermentation by yeast. For example, yeast extract, bran ingredients such as rice and wheat, vitamins, minerals, etc. can be used alone or in combination.
  • the beer-taste beverage of one aspect of the present invention may be a packaged beverage packed in a container.
  • Containers of any shape and material may be used for packaged beverages, and examples of containers include bottles, cans, barrels, and PET bottles. Bottles and PET bottles are preferred.
  • the method for producing a beer-taste beverage according to one aspect of the present invention is not particularly limited, but may be a method of production through a fermentation process or a method of production without a fermentation process. may Hereinafter, a method for producing a fermented beer-taste beverage produced through a fermentation process and a method for producing a non-fermented beer-taste beverage produced without undergoing a fermentation process will be described.
  • a method for producing a fermented beer-taste beverage according to one aspect of the present invention includes a method in which a fermentation step using yeast is performed. For example, the following steps (1) and (2).
  • the method for producing a fermented beer-taste beverage preferably further includes the following step (3).
  • - Process (3) A process of adjusting the turbidity of the beer-taste beverage after the process (2).
  • filtration through a filter with a pore size of 30 ⁇ m or more may or may not be performed.
  • the alcohol content, linalool content, sugar concentration, total polyphenol content, free amino acid content, and pH adjustment are performed according to (i) below. Although it can be performed at one or more timings of (v), the adjustment step is not essential.
  • step (2) and step (3) step (3)
  • Step (1) is a step of obtaining a pre-fermentation liquid by subjecting various raw materials to at least one of saccharification treatment, boiling treatment, and solid content removal treatment.
  • various raw materials including water and malt are put into a preparation pot or a preparation tank, and enzymes such as amylase are added as necessary.
  • various additives such as hops, preservatives, sweeteners, water-soluble dietary fibers, bittering agents or bitterness imparting agents, antioxidants, flavors, acidulants, and pigments may be added. These may be added before the saccharification treatment, may be added during the saccharification treatment, or may be added after the saccharification treatment is completed. Alternatively, these may be added after fermentation in the next step.
  • a mixture of various raw materials is subjected to a saccharification treatment by heating to saccharify the starch of the raw materials.
  • the temperature and time of saccharification treatment are determined by considering the type of malt used, the ratio of malt, water and raw materials other than malt, the type and amount of enzymes used, the concentration of raw wort extract in the final beverage, etc. Appropriate adjustment is preferable.
  • the saccharification temperature is 55 to 75° C.
  • the saccharification time is 30 to 240 minutes. is preferably
  • the saccharified liquid is preferably subjected to boiling treatment.
  • hops, a bittering agent, etc. as raw materials when performing this boiling treatment, it is preferable to add these. Hops, bittering agents, etc. may be added between the start of boiling of the saccharified liquid and before the end of boiling.
  • the boiling treatment After the boiling treatment is completed, it is preferably transferred to a whirlpool, cooled to 0 to 20° C. to obtain a cooling liquid, and then subjected to treatment for removing solids such as solidified protein.
  • the raw wort extract concentration can be adjusted to the range described above.
  • a pre-fermentation liquid is obtained.
  • filtration through a filter having a predetermined pore size for example, a pore size of less than 30 ⁇ m may be performed in order to remove solids.
  • a pre-fermentation solution may be prepared by adding hot water to malt extract, adding hops, a bittering agent, etc., and subjecting the mixture to boiling treatment.
  • malt is not used as various raw materials
  • liquid sugar containing carbon sources, nitrogen sources as amino acid-containing raw materials other than wheat or malt, hops, dietary fiber, preservatives, sweeteners, antioxidants, A bitterness-imparting agent, a flavoring agent, an acidulant, a pigment, etc. may be mixed with hot water to prepare a liquid sugar solution, and the liquid sugar solution may be subjected to a boiling treatment to prepare a pre-fermentation liquid.
  • hops When hops are used, they may be added before the boiling process, or may be added between the start of boiling the liquid sugar solution and before the end of boiling.
  • Step (2) is a step of adding yeast to the pre-fermentation liquid obtained in step (1) and performing fermentation.
  • the yeast used in this step can be appropriately selected in consideration of the type of fermented beverage to be produced, the desired flavor and fermentation conditions, etc. Top-fermenting yeast may be used, and bottom-fermenting yeast may be used. good.
  • Yeast may be added to the raw material solution as it is in yeast suspension, or a slurry obtained by concentrating the yeast by centrifugation or sedimentation may be added to the raw solution. Alternatively, after centrifugation, the supernatant may be completely removed and added.
  • the amount of yeast to be added to the undiluted solution can be appropriately set, and is, for example, about 5.0 ⁇ 10 6 cells/ml to 1.0 ⁇ 10 9 cells/ml.
  • the fermentation conditions for fermentation can be set as appropriate, but from the viewpoint of adjusting the sugar concentration and alcohol content of the beer-taste beverage to the above range, the fermentation temperature is preferably 5-25°C.
  • the sugar concentration and alcohol content of the beer-taste beverage can be adjusted by appropriately setting the type, amount and timing of addition of polysaccharide-degrading enzymes such as transglucosidase.
  • the temperature (temperature increase or decrease) or pressure of the fermented liquid may be changed during the process.
  • Step (3) is a step of adjusting the turbidity of the beer-taste beverage after step (2).
  • an aging treatment for aging the fermented liquid may be performed after the step (2).
  • By aging the fermented liquid it can be adjusted to a beer-taste beverage with high turbidity.
  • the beer-taste beverage may be heat-treated to adjust the invertase activity value to a predetermined range, or may be a beer-taste beverage without heat treatment.
  • the invertase activity value after heat treatment is 0 to 55 units, 0 to 50 units, 0 to 45 units, 0 to 40 units, 0 to 35 units, 0 to 30 units, 0 to 25 units, 0 It may be ⁇ 20 units, 0-15 units, or 0-10 units.
  • the above-mentioned "invertase activity value" serves as an index indicating the degree of heat load on the beer-taste beverage.
  • Inverdase is derived from yeast, and if heat treatment is not performed in a step after addition of yeast, inverdase is not deactivated. On the other hand, when the heat treatment is performed, the invertase is inactivated and the invertase activity value is lowered depending on the conditions of the heat treatment.
  • the invertase activity value can be adjusted by appropriately setting various conditions of the heat treatment (heating temperature, heating time, heating timing, etc.).
  • the invertase activity value can be measured by the following method.
  • acetic acid buffer A solution was prepared by dissolving 4.536 g of sodium acetate trihydrate (CH 3 COONa.3H 2 O) in about 350 mL of distilled water, and acetic acid was added to the solution while measuring the pH of the solution with a pH meter. About 1.9 mL of (CH 3 COOH) was added to adjust the pH to 4.5. Distilled water was then added to bring the volume to 500 mL to prepare an acetate buffer.
  • CH 3 COONa.3H 2 O sodium acetate trihydrate
  • Distilled water was then added to bring the volume to 500 mL to prepare an acetate buffer.
  • (substrate solution) 30.0 g of sucrose (sucrose) was dissolved in 125 mL of the acetate buffer prepared as described above, and distilled water was added to make the solution 500 mL to prepare a substrate solution.
  • (DNSA solution) 127.5 g of Rochelle salt (potassium sodium tartrate), 4.4 g of 3,5-dinitrosalicylic acid (DNSA), 3.5 g of phenol, and 3.5 g of sodium metabisulfite are dissolved in distilled water to a volume of 1 L, and DNSA is added.
  • a solution was prepared.
  • (2) Measurement method A control sample beverage was prepared in advance by heat-treating a beer-taste beverage to be measured at 100°C for 10 minutes.
  • Step (4), Step (5) when producing a non-alcoholic fermentation beer-taste beverage, it may be produced through a non-alcoholic fermentation process using yeast that does not produce alcohol. In this case, yeast that does not produce alcohol may be used in step (2) above. Further, when producing a non-alcoholic fermentation beer-taste beverage using alcohol-producing yeast, it is preferable to further perform steps (4) and (5) along with the above steps (1) to (3).
  • - Step (5) a step of adjusting the amount of carbon dioxide gas after step (4).
  • step (3) may be performed between step (2) and step (4), or performed between step (4) and step (5). It may be performed after step (5), but preferably after at least step (4).
  • the method of removing the alcohol produced by the fermentation step of step (2) is preferably a method of removing by heat treatment.
  • the conditions for the heat treatment the same conditions as in the general method for producing non-alcoholic beer-taste beverages can be applied.
  • alcohol is removed from the solution, and carbon dioxide gas is also removed. Therefore, it is preferable to adjust the amount of carbon dioxide by step (5).
  • a method for adjusting the amount of carbon dioxide gas it may be added by mixing the solution after step (4) with carbonated water, or carbon dioxide gas may be added directly to the solution after step (4). You may
  • the beer-taste beverage of one embodiment of the present invention thus obtained is filled in a predetermined container and distributed on the market as a product.
  • the method for packing the beer-taste beverage into a container is not particularly limited, and a container-packing method well known to those skilled in the art can be used.
  • the beer-taste beverage of the present invention is filled and sealed in a container by the container-packing step.
  • a container of any shape and material may be used in the container-packing step, and examples of the container include the container described in "1.4 Container-packed Beverage".
  • a method for producing a non-fermented beer-taste beverage according to one aspect of the present invention may be any method as long as it does not involve a fermentation step.
  • the step (a) is the same as the step (1) of obtaining the pre-fermentation liquid in the above-mentioned "method for producing a fermented beer-taste beverage".
  • the pre-beverage liquid obtained in step (a) may be added by mixing the pre-cooled pre-beverage liquid obtained in step (a) with carbonated water, or the carbon dioxide gas may be added to the undiluted cold beverage liquid. It may be added directly.
  • additives such as preservatives, sweeteners, flavors, acidulants, and pigments may be added, if necessary.
  • non-fermented beer-taste beverage is to be a non-fermented alcohol-containing beer-taste beverage
  • step (c) is included.
  • Step (c) may be performed at least after step (a), and may be performed, for example, at one or more timings of (I) to (III) below.
  • the alcohol component blended in step (c) spirits (distilled liquor) derived from the grains described above are preferable.
  • the turbidity, linalool content, sugar concentration, total polyphenol content, free amino acid content, and pH are adjusted according to the following (i ) to (v), but the adjustment step is not essential.
  • the turbidity depends on the type of raw material, the concentration of the original wort extract, the conditions of saccharification treatment in step (a) (heating temperature and heating time), and The conditions of the boiling treatment (heating temperature and heating time), the cooling rate of the pre-beverage liquid in step (b), the final cooling temperature, and the like can be adjusted.
  • the turbidity of the non-fermented beer-taste beverage can be adjusted by not filtering through a filter with a pore size of less than 30 ⁇ m in the manufacturing process.
  • solid content removal treatment to remove raw material residue such as malt used as a raw material, it is not limited to performing filtration through a filter with a predetermined pore size (for example, pore size of 30 ⁇ m or more).
  • the non-fermented beer-taste beverage obtained in this way is filled in a predetermined container and distributed on the market as a product.
  • the method for packing the non-fermented beer-taste beverage into a container is not particularly limited, and a container packing method well known to those skilled in the art can be used.
  • the container filling process the non-fermented beer-taste beverage is filled and sealed in the container.
  • a container of any shape and material may be used in the container filling step, and examples of the container are as described above.
  • Examples 1-13, Comparative Examples 1-11 Preparation of beverage> After putting the pulverized barley malt into a tank containing 40 L of hot water maintained at 40 to 55°C, a low temperature range of 40 to 55°C, a medium temperature range of 55 to 70°C, and a high temperature of 70 to 85°C. A saccharified solution was prepared by gradually increasing the temperature while maintaining each temperature range for a predetermined time (ranging from 1 minute to 60 minutes). After that, the malt cake was removed by filtration to obtain wort. Then, hops are further added to each wort and boiled, the wort after boiling is subjected to solid-liquid separation treatment, the obtained clear wort is cooled, yeast is added, and the fermentation temperature and Fermentation time was adjusted.
  • the beverages prepared in Examples 1 to 13 have a chewy texture and crispness suitable for beer-taste beverages, and the yeast odor and cosmetic aroma unsuitable for beer-taste beverages are suppressed.
  • the beverage prepared in Comparative Example 1 was inferior in texture suitable for beer-taste beverages.
  • the beverages prepared in Comparative Examples 2, 4, 6, 8, and 10 are beverages that have a yeasty odor that is unsuitable for beer-taste beverages, and the beverages prepared in Comparative Examples 3, 5, 7, 9, and 11 are , the beverage had a cosmetic aroma unsuitable for beer-taste beverages.

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Abstract

L'invention concerne une boisson aromatisée à la bière ayant une teneur en alcool inférieure ou égale à 5,0 % (v/v) et une turbidité supérieure ou égale à 22 Helm, et comprenant 120 µg/L ou moins de linalol.
PCT/JP2022/047034 2021-12-22 2022-12-21 Boisson aromatisée à la bière WO2023120560A1 (fr)

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