WO2022151575A1 - Procédé de classification et de traitement de fleur, et produit de fleur - Google Patents

Procédé de classification et de traitement de fleur, et produit de fleur Download PDF

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Publication number
WO2022151575A1
WO2022151575A1 PCT/CN2021/080357 CN2021080357W WO2022151575A1 WO 2022151575 A1 WO2022151575 A1 WO 2022151575A1 CN 2021080357 W CN2021080357 W CN 2021080357W WO 2022151575 A1 WO2022151575 A1 WO 2022151575A1
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Prior art keywords
flower
extractant
raw material
extract
active ingredients
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PCT/CN2021/080357
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English (en)
Chinese (zh)
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芦书峰
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芦书峰
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Priority claimed from CN202110063435.0A external-priority patent/CN112890191A/zh
Priority claimed from CN202110064854.6A external-priority patent/CN112868848A/zh
Application filed by 芦书峰 filed Critical 芦书峰
Publication of WO2022151575A1 publication Critical patent/WO2022151575A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives

Definitions

  • the present application relates to the technical field of flower products, in particular to a flower classification and processing technology and flower products.
  • Flowers are herbs with ornamental value, they are sun-loving and cold-resistant, short branches with reproductive function, and there are many kinds.
  • Flowers are composed of corolla, calyx, receptacle, and stamen. They have a variety of colors, grow in various shapes, and are scented or unscented.
  • Tea tree flowers are the flowers of the tea tree.
  • the tea tree generally blooms from October to January of the following year, and it has various colors such as white, yellow and pink. Flowers are monoecious.
  • tea tree flowers have been removed and discarded by tea farmers before they bloom because they compete with tea for nutrients, affecting the quality and yield of tea.
  • tea tree peanuts have a great effect in enhancing physical fitness, relieving fatigue, and improving immunity due to long-term growth, so they are gradually paying more attention.
  • the promotion of camellia flowers has been greatly restricted.
  • the existing flower classification and processing technology including the tea tree flower processing technology usually adopts the steps of water extraction, concentration and drying to prepare flower extracts including tea tree flower extracts, but this process has many disadvantages, such as low extraction efficiency and poor extraction quality. , low yield, poor crystal quality, etc., have become problems to be solved urgently.
  • the present application provides a flower classification processing technology and flower products to solve the technical defects existing in the prior art.
  • the application provides a flower classification processing technology, comprising the following steps:
  • S1 picking picking to obtain flower raw materials
  • S2 classification collect the image of the flower raw material, and determine the type of the flower raw material through the flower classification model based on the image of the flower raw material;
  • S4 concentration heat treatment and concentration treatment are successively performed on the extract to obtain a concentrated solution containing active ingredients of flowers;
  • S5 purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain a purified solution;
  • the S2 classification includes:
  • the flower classification model performs feature extraction on the image of the flower raw material to obtain feature information of the flower raw material
  • the active ingredients of flowers include but are not limited to: carbohydrates, proteins, and vitamins;
  • the extractant comprises a mineral extractant
  • the mineral extractant includes minerals with nano-microsphere structure, and the minerals include one or more of montmorillonite, diatomite, and sepiolite.
  • the extractant further includes an enzyme extractant, and the enzyme extractant includes an alkaline protease.
  • the S3 extraction includes:
  • the active ingredients in the flowers are extracted by immersing the flower raw materials in a corresponding solution containing an extractant to obtain an extract containing the active ingredients in the flowers.
  • the flower raw material is immersed in a solution containing an extractant, and the alkaline protease in it modifies and degrades the active ingredients in the flowers, so that the active ingredients in the flowers are extracted by minerals.
  • the mineral adsorption with nano-microsphere structure in the agent can extract the active ingredients in flowers.
  • the extract is heated to 80-100° C. and kept for 1.5-3 hours, so that the active ingredients of flowers adsorbed by the minerals are released, and then concentrated under reduced pressure. Concentration treatment to obtain a concentrated solution containing active ingredients of flowers;
  • the method of concentration under reduced pressure is as follows: in an environment with a temperature of 60-75° C. and a vacuum degree of 0.075-0.085 MPa, the extract is concentrated and processed until the relative density of the extract reaches 1.1 at the processing temperature. -1.15g/cm 3 , and then filtered through a 300-mesh shaking sieve to obtain a concentrated solution containing flower active ingredients.
  • the S5 purification includes: using a ceramic membrane to filter the concentrated solution, passing the obtained filtrate through a macroporous adsorption resin at a flow rate of 1-3BV/h, using water, 60%-70% ethanol, 3 %-5% alkali solution, 8%-10% acid solution, acetone elution, the eluate directly passes through the macroporous adsorption resin at a flow rate of 0.1-0.5BV/h, the effluent is collected, and concentrated to obtain a purified solution.
  • the S6 drying adopts a freeze-drying method, and the freeze-drying method includes a pre-freezing process and a drying process;
  • the pre-freezing process includes a cooling process and a maintenance process, wherein the cooling process is to cool the concentrated solution to the freezing point within 3 hours; the maintenance process is to maintain low-temperature freezing for 2-3 hours since the concentrated solution falls below the freezing point, Obtain the frozen product of the tea tree flower extract concentrate; monitor the core temperature of the concentrate during the pre-freezing stage to obtain the temperature value of the eutectic point of the concentrate;
  • the frozen material obtained in the freezing step is first evacuated, and then heated and dried.
  • the heating process is divided into low-temperature heating and high-temperature heating. , to obtain anhydrous frozen material; high temperature heating is to rapidly raise the temperature of the anhydrous frozen material to room temperature and maintain until the dry crystal of the tea tree flower extract is obtained, that is, the solid tea tree flower extract.
  • a filtering step is further included between the S3 extraction and the S4 concentration, and the filtering step includes: filtering the extract containing the active ingredients of Camellia sinensis by using a 70-90 mesh screen tube or a ceramic membrane.
  • the flower raw materials include but are not limited to: chrysanthemum, orchid, lotus, lily, plum, acacia, rose, apricot, daylily, hops, hollyhocks, peony, pear, sunflower, balsam, lemon Flower, peach, apricot, dandelion, rehmannia, celosia, dendrobium, canna, azalea, morning glory, persimmon, gorse, nasturtium, hibiscus, lavender, broccoli, geranium, calendula , night lilac, iris, pearl flower, daisy, pearl orchid, daisy flower, marigold, tuberose, cosmos, violet, epiphyllum, malan, platycodon, aloe, fern, mint, spearmint, wolfberry, shidia Cypress, bamboo shoots, hollyhock, impatiens, celosia, palm, chrysanthemum,
  • the flower raw material is Camellia sinensis
  • the flower extract is Camellia sinensis extract
  • the flower classification processing process only includes step S3 extraction to step S6 drying, namely:
  • Extraction obtaining the tea tree flower raw material, immersing the tea tree flower raw material in a solution containing an extractant to extract the active ingredient in the tea tree flower, and obtaining an extract containing the active ingredient of the tea tree flower;
  • the extract is successively subjected to heat treatment and concentration treatment to obtain a concentrated solution containing the active ingredients of Camellia sinensis;
  • Purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain purified solution;
  • Drying drying the purified liquid to obtain a solid tea tree flower extract.
  • the active ingredients of Camellia sinensis include but are not limited to: tea polyphenols, carbohydrates, tea proteins, vitamins, and caffeine.
  • the present application also provides a flower product, which contains the flower extract obtained by the flower classification and processing process described in any of the above paragraphs.
  • the flower products include solid or liquid food, beverages, condiments, daily necessities, cosmetics, smoking products, health products, medicines, feeds, and fertilizers.
  • foods include grain products, starch products, vegetable products, fruit products, meat products, poultry products, egg products, dairy products, candies, etc.; beverages include water, milk, wine, beverages, etc., wherein beverages include tea beverages , alcoholic beverages, carbonated beverages, etc.; daily necessities include hand sanitizer, hand cream, facial cleanser, body lotion, soap, talcum powder, toothpaste, tooth powder, oral cleaner, mouthwash, washing powder, laundry detergent, shampoo, hair care Vegetarian, facial tissue, sanitary napkins, diapers, etc.; Cosmetics include lotion, lotion, facial mask, eye mask, hand mask, essential oil, face cream, eye cream, acne remover, makeup remover, etc.; Tobacco products include flue-cured tobacco, sun cigarette, electronic cigarette, etc.
  • Condiments include salty agents, sour agents, sweeteners, umami agents, spicy agents, etc.
  • Health products include women's health products, men's health products, children's health products, elderly health products, etc.
  • Medicines include Chinese herbal medicines, Chinese herbal decoction pieces, Chinese patent medicines, Chinese and Western patent medicines, chemical raw materials and their preparations, antibiotics, biochemical drugs, radiopharmaceuticals, serums, vaccines, blood products, diagnostic drugs, etc.
  • feeds include roughage, green feed, silage, energy feed, and protein supplements Feed, mineral feed, vitamin feed, additives, etc.
  • fertilizers include quick-acting fertilizers, slow-acting fertilizers, controlled-release fertilizers, etc.
  • the flower product is a camellia flower product.
  • the tea tree flower product includes solid or liquid food, beverages, condiments, daily necessities, cosmetics, smoking products, health products, medicines, feeds, and fertilizers.
  • the flower classification and processing technology provided by the present application classifies the flower raw materials through the flower classification model, which can accurately and quickly determine the type of the flower raw material, and then innovatively uses solutions containing different extracting agents to extract the flower raw materials, that is, for different types of flowers. Different extraction measures are taken to realize individualized extraction of the flowers, which greatly improves the extraction efficiency and extraction quality.
  • the extractant is preferably a mineral extractant.
  • the mineral extractant can utilize the nano-microsphere structure of its minerals to adsorb the active ingredients of flowers, so that the active ingredients of flowers are adsorbed in its structure, and the After the extraction is completed, high temperature heating is used to release the adsorbed active ingredients of flowers, thereby improving the extraction efficiency and quality of active ingredients of flowers.
  • the extractant may also include an enzyme extractant, and the enzyme extractant and the mineral extractant complement each other and cooperate with each other, which can further promote the extraction of the active ingredients of flowers.
  • the enzyme extractant can modify and degrade the active ingredients such as proteins and sugars in the flowers, and promote the removal of these macromolecular active ingredients by the minerals containing nano-microsphere structure in the mineral extractant. Adsorption, and then heat treatment to release it into the solution, thereby further improving the extraction efficiency and extraction quality of the active ingredients of flowers.
  • the tea tree flower processing technology provided by the application innovatively uses a solution containing an extractant to extract the tea tree flower raw material, which can greatly improve the extraction efficiency and extraction quality, increase the scope of application, good crystallization effect, low cost and high economic benefit.
  • the extractant is preferably a mineral extractant.
  • the mineral extractant can utilize the nano-microsphere structure of its minerals to adsorb the active ingredients of the tea tree flowers, so that the active ingredients of the tea tree flowers are adsorbed within its structure.
  • the effective components of the tea tree flower adsorbed are released by means of high temperature heating, thereby improving the extraction efficiency and quality of the effective components of the tea tree flower.
  • the extractant may also include an enzyme extractant, and the enzyme extractant and the mineral extractant complement each other and cooperate with each other, which can further promote the extraction of the effective components of the tea tree flower.
  • the enzyme extractant can modify and degrade the macromolecular active ingredients such as proteins and sugars in the camellia flowers, and promote the removal of these macromolecular active ingredients by the mineral extractants containing nano-microspheres. Mineral adsorption of the structure, and then heat treatment to release it into the solution, thereby further improving the extraction efficiency and extraction quality of the active ingredients of the tea tree flower.
  • the flower products provided by the application are rich in nutrients and extremely low in water content, and can meet the needs of various groups of people of different ages for eating and drinking.
  • the tea tree flower product provided by the present application is rich in nutrients and extremely low in water content, and can meet the needs of various age groups and different groups of people for eating and drinking.
  • Fig. 1 is the flower classification processing process flow chart of an embodiment of the present application
  • Fig. 2 is the flow chart of the tea tree flower processing process steps of an embodiment of the present application
  • Fig. 3 is the Camellia flower product composition-efficacy resonance wave frequency detection figure of an embodiment of the present application
  • Fig. 4 is the Camellia flower product composition-functional resonance wave frequency detection diagram of an embodiment of the present application.
  • Fig. 5 is the Camellia flower product composition-organ resonance wave frequency detection diagram of an embodiment of the present application.
  • Fig. 6 is the Camellia flower product composition-system resonance wave frequency detection diagram of an embodiment of the present application.
  • Fig. 7 is the resonance analysis detection figure of the tea polyphenol component in the tea tree flower product of an embodiment of the present application.
  • Fig. 8 is the resonance analysis detection diagram of other components in the camellia flower product of an embodiment of the present application.
  • Fig. 9 is the yin-yang, qi machine, five-element resonance wave frequency detection diagram of the Camellia sinensis product of an embodiment of the present application.
  • FIG. 10 is a diagram showing the medicinal properties, five flavors, efficacy, and meridian resonance wave frequencies of a tea tree flower product according to an embodiment of the present application.
  • This embodiment provides a flower classification and processing process, as shown in FIG. 1 , including steps S1 to S6.
  • flower raw materials include but are not limited to: chrysanthemum, orchid, lotus, lily, plum, acacia, rose, apricot, daylily, hops, hollyhocks, peony, pear, sunflower, balsam, lemon blossom, peach blossom , apricot, dandelion, rehmannia, celosia, dendrobium, canna, azalea, morning glory, persimmon, gorse, nasturtium, hibiscus, lavender, broccoli, geranium, calendula, night lilac , iris, pearl flower, daisy, pearl orchid, daisy flower, marigold, tuberose, cosmos, violet, epiphyllum, malan, platycodon, aloe vera, fern, mint, spearmint, wolfberry, cypress, bamboo shoots , hollyhock, balsam, celosia, palm, chry
  • S2 classification collect the image of the flower raw material, and determine the type of the flower raw material through the flower classification model based on the image of the flower raw material.
  • the image of the flower raw material can be collected, and the image of the flower raw material can be input into the flower classification model; the flower classification model can perform feature extraction on the image of the flower raw material to obtain the feature information of the flower raw material;
  • the flower raw material characteristic information is matched with the pre-stored flower type characteristic information, and the type of the flower raw material is determined.
  • the characteristic information of the flower raw material includes but is not limited to flower color, number of petals, shape of petals, shape of filament, whether there are side thorns, etc.
  • the flower classification model can be obtained by training, and the training method includes the following steps:
  • sample feature vector (2) Feature extraction is performed on the training samples through the flower classification model to obtain sample feature information (sample feature vector);
  • the loss value of the loss function can indicate the degree of similarity between the prediction result and the sample label, that is, the accuracy of the model
  • the preset threshold can divide the optimal range of the degree of similarity between the prediction result and the sample label.
  • the active ingredients of flowers include but are not limited to: carbohydrates, proteins, and vitamins.
  • dry matter, total flavonoids, anthocyanins, dietary fiber and the like may also be included.
  • the extractant includes a mineral extractant; the mineral extractant includes a mineral with a nano-microsphere structure, and the mineral includes one or more of montmorillonite, diatomite, and sepiolite.
  • mineral raw materials such as montmorillonite, diatomite, and sepiolite can be ground, sieved, and purified to obtain mineral raw material powder, and then the mineral raw material powder can be dissolved in water, inorganic solvent or organic solvent. , to prepare a solution containing the above-mentioned mineral extractant.
  • the ratio between mineral raw material powder and water, inorganic solvent or organic solvent can be 10-30:70-90, that is, the mass fraction of mineral raw material powder can be 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, etc.
  • the mass fraction of water or organic solvent can be 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, etc.
  • the organic solvent can be ethanol, diethyl ether, isopropanol, N,N-dimethylacetamide, ethyl acetate Glycol, diethylene glycol, glycerol, acetamide, etc.
  • the inorganic solvent can be acid solution, alkali solution, salt solution, etc., which are not limited in this application.
  • Minerals such as montmorillonite, diatomite, and sepiolite selected in this example have nanometer diameters of 1-80 ⁇ m (such as 1 ⁇ m, 5 ⁇ m, 10 ⁇ m, 20 ⁇ m, 30 ⁇ m, 40 ⁇ m, 50 ⁇ m, 60 ⁇ m, 70 ⁇ m, 80 ⁇ m, etc.).
  • Microsphere structure a certain void structure will be formed between adjacent nano-microspheres. After these minerals are dissolved in water or organic solvents, especially after micro-heating (40-60°C), the above-mentioned nano-microspheres have The void structure will be slightly enlarged, and the active ingredients of flowers will be adsorbed into it. After high temperature heating (80-100°C), the void structure will continue to enlarge, and the adsorbed active ingredients of flowers will be released.
  • the extractant also includes an enzyme extractant.
  • the enzyme extractant is preferably alkaline protease.
  • the alkaline protease may be Novo protease, Carsberg protease, etc., which is not limited in this application.
  • the composition of the flower raw material can be determined based on the type of the flower raw material, and the extractant of the flower raw material can be determined based on the composition of the flower raw material.
  • the corresponding extractant can be determined based on the protein content of the flower raw material.
  • the corresponding extractant preferably includes 0.1-3 parts by mass (such as 0.1, 0.5, 1, 1.5, 2 , 2.5, 3 parts by mass) of the enzyme extractant and 97-99.9 parts by mass (such as 97, 97.5, 98, 98.5, 99, 99.5, 99.9 parts by mass) of the mineral extractant;
  • its corresponding extractant preferably includes 4-6 parts by mass (such as 4, 4.5, 5, 5.5, 6 parts by mass) parts) of the enzyme extractant and 94-96 parts by mass (such as 94, 94.5, 95, 95.5, 96 parts by mass) of the mineral extractant;
  • its corresponding extractant preferably includes 7-9 parts by mass (such as 7, 7.5, 8, 8.5, 9 parts by mass) parts) of the enzyme extractant and 91-93 parts by mass (such as 91, 91.5, 92, 92.5, 93 parts by mass) of the mineral extractant;
  • its corresponding extractant preferably includes 10-15 parts by mass (such as 10, 11, 12, 13, 14, 15 parts by mass) of the enzyme extractant and 85-90 parts by mass (such as 85, 86, 87, 88, 89, 90 parts by mass) of the mineral extractant;
  • its corresponding extractant preferably includes 16-22 parts by mass (such as 16, 17, 18, 19, 20, 21, 22 parts by mass) of the enzyme extractant and 78-84 parts by mass.
  • Mineral extractant in parts by mass eg 78, 79, 80, 81, 82, 83, 84 parts by mass).
  • the enzyme extractant can be regarded as a degrading agent for the active ingredients of flowers, and the mineral extractant can be regarded as the extraction carrier of the active ingredients of flowers.
  • the enzyme extractant can modify and degrade the macromolecular active ingredients such as protein in flowers, so that the peptide chain becomes shorter and the molecular weight becomes smaller, and at the same time, it can also degrade other macromolecular active ingredients connected to the protein, etc., Thereby, the active ingredients such as tea polyphenols, sugars, tea proteins, vitamins, and caffeine in the flowers are changed into smaller molecules, which are more easily absorbed by the montmorillonite, diatomite, Sepiolite and other components are adsorbed and then extracted.
  • the protein molecular weight is usually relatively large, it is difficult to be adsorbed by the mineral extractant. Therefore, when the protein content in flowers is high, the content of the enzyme extractant is correspondingly increased, which is conducive to the sufficient degradation of the protein component by the enzyme extractant. Modification, which in turn promotes its adsorption and extraction by mineral extractants, and improves the extraction efficiency, and vice versa.
  • the flower raw material after the flower raw material is immersed in the extractant, it may be subjected to heat treatment and/or pH adjustment treatment to further enhance the degradation effect of the enzyme extractant and the adsorption effect of the mineral extractant.
  • the heat treatment can be heating the extractant immersed in the flower raw material to 40-60°C (such as 40°C, 45°C, 50°C, 55°C, 60°C, etc.) for 1-2 hours;
  • the pH adjustment treatment can be adding The alkaline solution adjusts the pH value of the extractant immersed in the flower raw material in the range of 8-10 (eg, 8, 8.5, 9, 9.5, 10, etc.).
  • S4 concentration heat treatment and concentration treatment are sequentially performed on the extraction solution to obtain a concentrated solution containing active ingredients of flowers.
  • the extract can be heated to 80-100° C. and kept for 1.5-3 hours to release the active ingredients of flowers adsorbed by the minerals, and then concentrated to obtain a concentrated solution containing active ingredients of flowers.
  • the heating temperature of the extraction solution can be 80°C, 85°C, 90°C, 95°C, 100°C, etc.
  • the heating time can be 1.5h, 2h, 2.5h, 3h, etc., which is not limited in this application.
  • the extract can be concentrated by a vacuum concentration method to obtain a concentrated solution containing the active ingredients of flowers.
  • the vacuum concentration method is specifically as follows: the temperature is 60-75°C, and the vacuum degree is 0.075-0.085MPa.
  • other existing concentration methods can also be used for concentration treatment, such as reverse osmosis concentration method, etc., which are not limited in this application, and will not be repeated here.
  • a filtering step may be included between the S3 extraction and the S4 concentration, and the filtering step preferably includes: filtering the extract containing the active ingredients of Camellia sinensis by using a 70-90 mesh screen tube or a ceramic membrane. This filtration step can filter out macromolecular impurities in the liquid before concentration, thereby further improving the purity of the extract.
  • S5 purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain a purified solution.
  • a ceramic membrane can be used to filter the concentrated solution, and the obtained filtrate is passed through a macroporous adsorption resin at a flow rate of 1-3BV/h, using water, 60%-70% ethanol, and 3%-5% alkaline solution respectively. , 8%-10% acid solution, acetone elution, the eluent directly passes through the macroporous adsorption resin at a flow rate of 0.1-0.5BV/h, the effluent is collected, and concentrated to obtain a purified solution.
  • the ceramic membrane is densely covered with micropores, and the permeability is different according to the different molecular diameters of the substances permeating within a certain membrane pore size range.
  • the pressure difference on both sides of the membrane is the driving force, and the membrane is the filter medium.
  • the membrane is the filter medium.
  • Macroporous adsorption resin is a kind of polymer adsorption resin without exchange group and macroporous structure. It has good macroporous network structure and large specific surface area. It can selectively adsorb organic matter in aqueous solution through physical adsorption.
  • the macroporous adsorption resin is eluted by water, 60%-70% ethanol, 3%-5% alkaline solution, 8%-10% acid solution, and acetone, the active ingredient proteins and carbohydrates in flowers flow out. Concentrate again to obtain purified liquid.
  • the drying step preferably adopts a freeze-drying method, and the freeze-drying method includes a pre-freezing process and a drying process.
  • the pre-freezing process includes a cooling process and a maintenance process, wherein the cooling process is to cool the concentrated solution to the freezing point within 3 hours; the maintenance process is to maintain the low temperature freezing for 2-3 hours from the time the concentrated solution falls below the freezing point to obtain flowers. Extract the frozen material of the concentrated liquid; monitor the core temperature of the concentrated liquid during the pre-freezing stage to obtain the temperature value of the eutectic point of the concentrated liquid.
  • the frozen material obtained in the freezing step is first evacuated, and then heated and dried.
  • the heating process is divided into low-temperature heating and high-temperature heating. , to obtain anhydrous frozen material; high-temperature heating is to rapidly raise the temperature of the anhydrous frozen material to room temperature and maintain until the dry crystal of flower extract is obtained, that is, solid flower extract.
  • part of the mineral extractant remaining in the purified liquid can absorb the heat of the liquid, thereby promoting the crystallization of the purified liquid, accelerating drying, and improving the crystal quality and crystal uniformity.
  • the flower processing technology provided by the application innovatively adopts the extraction agent to extract the flower raw material, can greatly improve the extraction efficiency and extraction quality, increase the scope of application, good crystallization effect, low cost and high economic benefit.
  • the flower raw material is Camellia sinensis
  • the flower extract is Camellia sinensis extract.
  • the flower processing technique namely the Camellia sinensis processing technique, comprises the following steps:
  • S1 extraction immersing the tea tree flower raw material in a solution containing an extractant to extract the active ingredients in the tea tree flowers to obtain an extract containing the active ingredients of the tea tree flowers.
  • the tea tree flower raw material can be obtained by picking, and the tea tree flower raw material is the flower bud of a pure natural and pollution-free wild-type ancient tree or an ancient tree of large-leaf species, and the flower bud is a tea tree flower with buds waiting to be released or the petals just leaving the core state, 2-3 days before and after the pollination period, picking around October to November every year.
  • Camellia sinensis include but are not limited to: tea polyphenols, carbohydrates, tea proteins, vitamins, and caffeine.
  • dry matter, total flavonoids, tea saponins, dietary fibers, tea polysaccharides and the like may also be included.
  • the extractant includes a mineral extractant
  • the mineral extractant includes a mineral with a nano-microsphere structure
  • the above-mentioned mineral is preferably one or more of montmorillonite, diatomite, and sepiolite.
  • mineral raw materials such as montmorillonite, diatomite, and sepiolite can be ground, sieved, and purified to obtain mineral raw material powder, and then the mineral raw material powder can be dissolved in water, inorganic solvent or organic solvent. , to prepare a solution containing the above-mentioned mineral extractant.
  • the ratio between mineral raw material powder and water, inorganic solvent or organic solvent can be 10-30:70-90, that is, the mass fraction of mineral raw material powder can be 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, etc.
  • the mass fraction of water or organic solvent can be 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, etc.
  • the organic solvent can be ethanol, diethyl ether, isopropanol, N,N-dimethylacetamide, ethyl acetate Glycol, diethylene glycol, glycerol, acetamide, etc.
  • the inorganic solvent can be acid solution, alkali solution, salt solution, etc., which are not limited in this application.
  • Minerals such as montmorillonite, diatomite, and sepiolite selected in this example have nanometer diameters of 1-80 ⁇ m (such as 1 ⁇ m, 5 ⁇ m, 10 ⁇ m, 20 ⁇ m, 30 ⁇ m, 40 ⁇ m, 50 ⁇ m, 60 ⁇ m, 70 ⁇ m, 80 ⁇ m, etc.).
  • Microsphere structure a certain void structure will be formed between adjacent nano-microspheres. After these minerals are dissolved in water or organic solvents, especially after micro-heating (40-60°C), the above nano-microspheres will appear The void structure will be slightly enlarged, and the active ingredients of camellia flower will be adsorbed into it. After high temperature heating (80-100°C), the void structure will continue to enlarge, and the adsorbed active ingredients of camellia flower will be released.
  • the extractant also includes an enzyme extractant.
  • the enzyme extractant is preferably alkaline protease.
  • the alkaline protease may be Novo protease, Carsberg protease, etc., which is not limited in this application.
  • the enzyme extractant can be regarded as a degrading agent for the active ingredients of the camellia flower, and the mineral extractant can be regarded as the extraction carrier of the active constituents of the camellia flower.
  • the two complement each other and cooperate with each other.
  • the enzyme extractant can modify and degrade the macromolecular active components such as proteins and carbohydrates in camellia flowers, so that the peptide chains are shortened and the molecular weight is reduced.
  • tea polyphenols, sugars, tea proteins, vitamins, caffeine and other active ingredients in tea tree flowers into smaller molecules, which are more easily absorbed by the mineral extractant containing nano-microspheres
  • the structural montmorillonite, diatomite, sepiolite and other components are adsorbed and then extracted.
  • the ratio of the enzyme extractant to the mineral extractant may be 1-5:95-99. That is, the enzyme extractant can be 1 part by mass, 2 parts by mass, 3 parts by mass, 4 parts by mass, 5 parts by mass, etc., and the mineral extractant can be 95 parts by mass, 96 parts by mass, 97 parts by mass, etc. parts, 98 parts by mass, 99 parts by mass, etc.
  • the tea tree flower raw material after the tea tree flower raw material is immersed in the extractant, it can be subjected to heat treatment and/or pH adjustment treatment to further enhance the degradation effect of the enzyme extractant and the adsorption effect of the mineral extractant.
  • the heat treatment can be heating the extractant immersed in the raw material of camellia flower to 40-60°C (such as 40°C, 45°C, 50°C, 55°C, 60°C, etc.) for 1-2 hours;
  • the pH adjustment treatment can be The alkaline solution is added to adjust the pH value of the extractant immersed in the raw material of camellia flower in the range of 8-10 (eg, 8, 8.5, 9, 9.5, 10, etc.).
  • the extract can be heated to 80-100° C. and kept for 1.5-3 hours to release the active ingredients of camellia flowers adsorbed by the minerals, and then subjected to concentration treatment to obtain a concentrated solution containing active ingredients of camellia flowers .
  • the heating temperature of the extraction solution can be 80°C, 85°C, 90°C, 95°C, 100°C, etc.
  • the heating time can be 1.5h, 2h, 2.5h, 3h, etc., which is not limited in this application.
  • the extract can be concentrated by a vacuum concentration method to obtain a concentrated solution containing the active ingredients of Camellia sinensis.
  • other existing concentration methods can also be used for concentration treatment, such as reverse osmosis concentration method, etc., which are not limited in this application, and will not be repeated here.
  • a filtering step may be included between the S1 extraction and the S2 concentration, and the filtering step preferably includes: filtering the extract containing the active ingredients of Camellia sinensis by using a 70-90 mesh screen tube or a ceramic membrane. This filtration step can filter out macromolecular impurities in the liquid before concentration, thereby further improving the purity of the extract.
  • a ceramic membrane can be used to filter the concentrated solution, and the obtained filtrate is passed through a macroporous adsorption resin at a flow rate of 1-3BV/h, using water, 60%-70% ethanol, and 3%-5% alkaline solution respectively. , 8%-10% acid solution, acetone elution, the eluent directly passes through the macroporous adsorption resin at a flow rate of 0.1-0.5BV/h, the effluent is collected, and concentrated to obtain a purified solution.
  • the ceramic membrane is densely covered with micropores, and the permeability is different according to the different molecular diameters of the substances permeating within a certain membrane pore size range.
  • the pressure difference on both sides of the membrane is the driving force, and the membrane is the filter medium.
  • the membrane is the filter medium.
  • Macroporous adsorption resin is a kind of polymer adsorption resin without exchange group and macroporous structure. It has good macroporous network structure and large specific surface area. It can selectively adsorb organic matter in aqueous solution through physical adsorption.
  • the macroporous adsorption resin is eluted by water, 60%-70% ethanol, 3%-5% alkali solution, 8%-10% acid solution, and acetone, the active ingredients of tea polyphenols, sugars, tea Protein, vitamins, caffeine, etc. flow out, and then concentrated again to obtain purified liquid.
  • the drying step preferably adopts a freeze-drying method, and the freeze-drying method includes a pre-freezing process and a drying process.
  • the pre-freezing process includes a cooling process and a maintenance process, wherein the cooling process is to cool the concentrated solution to the freezing point within 3 hours; the maintenance process is to maintain the low temperature freezing for 2-3 hours from the time the concentrated solution falls below the freezing point to obtain the tea tree.
  • the frozen material of the concentrated liquid of flower extraction; the central temperature of the concentrated liquid is monitored during the pre-freezing stage, and the temperature value of the eutectic point of the concentrated liquid is obtained.
  • the frozen material obtained in the freezing step is first evacuated, and then heated and dried.
  • the heating process is divided into low-temperature heating and high-temperature heating. , to obtain anhydrous frozen material; high temperature heating is to rapidly raise the temperature of the anhydrous frozen material to room temperature and maintain until the dry crystal of the tea tree flower extract is obtained, that is, the solid tea tree flower extract.
  • part of the mineral extractant remaining in the purified liquid can absorb the heat of the liquid, thereby promoting the crystallization of the purified liquid, accelerating drying, and improving the crystal quality and crystal uniformity.
  • the tea tree flower processing technology provided in this embodiment innovatively uses an extractant to extract the tea tree flower raw material, which can greatly improve the extraction efficiency and extraction quality, increase the scope of application, good crystallization effect, low cost and high economic benefit.
  • the Camellia sinensis product containing the Camellia sinensis extract prepared in this example was subjected to product component-efficacy resonant wave frequency detection, and the results are shown in Figure 3 .
  • the high-frequency resonance of the efficacy of tea tree flower products mainly occurs in three types of components: tea polyphenols, tea polysaccharides, and tea proteins. Therefore, its main efficacy is determined by the wave frequencies of these three components. Decide.
  • tea saponins and caffeine also play an important role in efficacy, so the relationship between these five components and efficacy is analyzed in detail.
  • Figures 4-6 are respectively the resonant wave frequency diagrams of each component, function, organ, and system in the Camellia sinensis product provided in this embodiment. It can be seen that the efficacy of tea polyphenols, tea polysaccharides, tea protein, tea saponin and caffeine in tea tree flower products.
  • Tea polyphenols can scavenge free radicals, anti-oxidation, anti-aging, improve cell activity, enhance cell repair ability, anti-mutation, anti-tumor, improve immunity, anti-radiation, anti-allergic, and can also protect the heart, liver, spleen, gallbladder, Prostate, testicular function, protection of circulation, microcirculation, lymphatic circulation, blood system, vascular system, etc.
  • Tea polysaccharides can be anti-oxidative, anti-tumor, improve immunity, and can also protect heart, liver, kidney, pancreas, bladder function, protect gynecological nourishing function, protect circulatory system, coronary circulatory system, digestive system, endocrine system, blood vessels, etc.
  • Tea protein can scavenge free radicals, resist oxidation, improve cell activity, prevent radiation, and can also protect the brain, heart, lungs, liver, stomach, small intestine, gallbladder, uterus, breast function, and protect the brain, coronary circulation, digestive system, Endocrine system, blood system, vascular system, etc.
  • Tea saponin can improve cell activity, protect stomach and large intestine function, and also protect circulatory system, digestive system, blood vascular system, etc.
  • Caffeine can improve cell activity, enhance the vitality of the brain, heart and lungs, enhance the vitality of kidneys, small intestine and prostate, activate brain nerves, and accelerate microcirculation.
  • the content of CAT is higher than the benchmark, so it highlights the superior anti-oxidation, anti-aging, anti-tumor, regulating blood lipid, regulating blood sugar, anti-thrombosis, protecting the function of the cardiovascular system and relieving diabetes.
  • this product also has the effect of activating brain cells, improving memory and concentration, relieving stress, and calming the mind.
  • the caffeine content is significantly lower than that of tea leaves, so the taste is not astringent, which improves the mental state without causing stress on the heart and brain.
  • Figure 9a is the yin and yang resonance wave frequency diagram of the tea tree flower product, it can be seen that the yin and yang of the tea tree flower product are basically balanced, can nourish both yin and yang, and have the characteristics of high-quality health products;
  • Figure 9b is the qi of the tea tree flower product. From the resonance wave frequency diagram of the machine, it can be seen that the tea tree flower product has sufficient gas power and belongs to the upper-class health product level;
  • Figure 9c is the resonance wave frequency diagram of the gas machine category of the tea tree flower product. It can be seen that the tea tree flower product has the most function of nourishing vitality.
  • Figure 9d is the resonant wave frequency diagram of the five elements of the tea tree flower product, it can be seen that the tea tree flower product Qi It is biased towards wood, water and fire, among which wood is the strongest, so it has superior functions such as dredging and penetrating, nourishing yin and tonifying yang.
  • Figure 10a is the resonant wave frequency diagram of the medicinal properties of the tea tree flower product, it can be seen that the tea tree flower product is flat and cool
  • Figure 10b is the five-flavor resonance wave frequency diagram of the tea tree flower product, it can be seen that the tea tree flower product has a slightly bitter taste Gan
  • Figure 10c is the efficacy of the tea tree flower product - meridian resonance wave frequency diagram. It can be seen that the tea tree flower product has very significant effects on the heart, lung, stomach, small intestine meridian, liver, kidney, and pericardium meridian.
  • the tea tree flower product provided in this example can remove free radicals in the body, improve anti-oxidation, anti-aging and anti-tumor capabilities; can enhance cell activity and repair ability, especially liver cells, improve anti-virus and immune functions; can regulate Balance blood lipids/blood sugar/blood pressure, remove thrombus, uric acid and various toxin deposits; can protect cardiovascular and cerebrovascular, prevent coronary sclerosis, cerebral infarction, relieve and prevent diabetes; can enhance cranial nerve vitality and feedback speed, enhance memory, focus and relieve Mental stress; can also repair damaged liver cells, protect the liver, pancreas, stomach and promote digestive function.
  • the flower products provided by the present application are rich in nutrients and extremely low in water content, and can meet the edible needs of different groups of people of different ages.
  • specific content of other flower products please refer to the above-mentioned tea tree flower products, which will not be repeated here.
  • test groups 1-5 are set. Test groups 1-5 were all prepared by the following process to obtain flower extracts. In this test example, roses were used as flowers (the rosette protein content was about 8.5%).
  • Extraction obtaining flower raw materials, immersing the flower raw materials in a solution containing an extractant to extract the active ingredients in the flowers, and obtaining an extract containing the active ingredients of the flowers;
  • the extraction solution is successively subjected to heat treatment and concentration treatment to obtain a concentrated solution containing active ingredients of flowers;
  • Purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain purified solution;
  • Drying drying the purified solution to obtain a solid flower extract.
  • the difference between the 5 groups is that the extractant used in test group 1 is water, the extractant used in test group 2 is pure mineral extractant, and the extractant used in test group 3 is 99 parts of mineral extractant + 1 part of enzyme extractant , the extractant used in test group 4 is 95 parts of mineral extractant + 5 parts of enzyme extractant, and the extractant used in test group 5 is 88 parts of mineral extractant + 12 parts of enzyme extractant.
  • test group 4 adopts a suitable proportion of mineral extractant and enzyme extractant to extract together, which can greatly improve the content of protein, vitamin C and glucose in the rose extract, and improve its extraction efficiency.
  • group 2 experimental group 3 and experimental group 5, the proportion of pure mineral extractant or enzyme extractant is too high or too low, the content of protein, vitamin C and glucose in rose flower extract has a certain increase, but the effect is not as good as The test group 3 was significantly, while the test group 1 only used water extraction, and the content of protein, vitamin C, and glucose in the rosette extract was lower.
  • test groups 1-5 are set. Test groups 1-5 were all prepared by the following process to obtain flower extracts. In this test example, chrysanthemum was used for flowers (the protein content of chrysanthemum was about 13.6%).
  • Extraction obtaining flower raw materials, immersing the flower raw materials in a solution containing an extractant to extract the active ingredients in the flowers, and obtaining an extract containing the active ingredients of the flowers;
  • the extraction solution is successively subjected to heat treatment and concentration treatment to obtain a concentrated solution containing active ingredients of flowers;
  • Purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain purified solution;
  • Drying drying the purified solution to obtain a solid flower extract.
  • the difference between the 5 groups is that the extractant used in test group 1 is water, the extractant used in test group 2 is pure mineral extractant, and the extractant used in test group 3 is 96 parts of mineral extractant + 4 parts of enzyme extractant , the extractant used in test group 4 is 92 parts of mineral extractant + 8 parts of enzyme extractant, and the extractant used in test group 5 is 88 parts of mineral extractant + 12 parts of enzyme extractant.
  • test group 4 uses a suitable proportion of mineral extractant and enzyme extractant to extract together, which can greatly improve the content of protein, trace element calcium and trace element potassium in the chrysanthemum extract, and improve its extraction efficiency.
  • test group 2, test group 3 and test group 5 the proportion of pure mineral extractant or enzyme extractant is too high or too low, the content of protein, trace element calcium, and trace element potassium in chrysanthemum extract has a certain increase , but the effect was not as significant as that of test group 3, while test group 1 only used water extraction, and the contents of protein, trace element calcium and trace element potassium in chrysanthemum extract were lower.
  • test groups 1-4 are set. Test groups 1-4 all adopt the following process to prepare the tea tree flower extract:
  • Extraction obtaining the tea tree flower raw material, immersing the tea tree flower raw material in a solution containing an extractant to extract the active ingredient in the tea tree flower, and obtaining an extract containing the active ingredient of the tea tree flower;
  • the extract is successively subjected to heat treatment and concentration treatment to obtain a concentrated solution containing the active ingredients of Camellia sinensis;
  • Purification use ceramic membrane and macroporous adsorption resin to purify the concentrated solution to obtain purified solution;
  • Drying drying the purified liquid to obtain a solid tea tree flower extract.
  • the difference between the 4 groups is that the extractant used in test group 1 is water, the extractant used in test group 2 is mineral extractant, the extractant used in test group 3 is enzyme extractant, and the extractant used in test group 4 is mineral extractant. Substance extractant + enzyme extractant.
  • test group 4 uses mineral extractant and enzyme extractant to extract together, which can greatly increase the content of tea polyphenols, caffeine and theanine in the tea tree flower extract, and improve the extraction efficiency.
  • the content of tea polyphenols, caffeine, and theanine in the tea tree flower extract was increased to a certain extent in group 2 only with mineral extractant, while experimental group 3 and test group 1 only used enzyme extractant or water extraction, and tea tree flower extract was used for extraction.
  • the content of tea polyphenols, caffeine, and theanine is low.
  • test group 4 adopts mineral extractant and enzyme extractant to extract together, which can greatly improve the content of sugars, dry matter and total flavonoids in the tea tree flower extract, and improve its extraction efficiency.
  • Group 2 only used mineral extractant for extraction, and the content of sugar, dry matter and total flavonoids in tea tree flower extract increased to a certain extent, while test group 3 and test group 1 only used enzyme extractant or water extraction, tea tree flower extract The content of sugar, dry matter and total flavonoids is low.
  • the difference between the control group and the test group is that the extractant used in the control group is water, while the extractant used in the test group is a mineral extractant + an enzyme extractant.
  • ND means not detected.

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Abstract

L'invention concerne un procédé de classification et de traitement de fleur, ainsi qu'un produit de fleur. Le procédé de classification et de traitement de fleur selon l'invention comprend les étapes suivantes : S1 ramassage : ramasser et obtenir une matière première de fleur ; S2 classification : acquérir une image de la matière première de fleur et déterminer le type de matière première de fleur au moyen d'un modèle de classification de fleur, en fonction de l'image de ladite matière première ; S3 extraction: selon le type de matière première, immerger la matière première de fleur dans une solution correspondante contenant un agent d'extraction pour extraire un composant actif de la fleur et obtenir une solution d'extraction contenant le composant actif de la fleur ; S4 concentration : chauffer et concentrer de façon séquentielle la solution d'extraction pour obtenir une solution concentrée contenant le composant actif de la fleur ; S5 purification : purifier la solution concentrée au moyen d'une membrane céramique et d'une résine d'adsorption macro-poreuse pour obtenir une solution purifiée ; et S6 séchage : sécher la solution purifiée pour obtenir un extrait de fleur solide. Le procédé de classification et de traitement de fleur selon l'invention permet de réaliser une extraction personnalisée, d'améliorer l'efficacité d'extraction et la qualité d'extraction, et d'obtenir un avantage économique élevé.
PCT/CN2021/080357 2021-01-18 2021-03-12 Procédé de classification et de traitement de fleur, et produit de fleur WO2022151575A1 (fr)

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CN118059005A (zh) * 2024-04-22 2024-05-24 广州巴宝莉化妆品有限公司 一种鲜花花香提取物的持久留香的方法
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CN118059005A (zh) * 2024-04-22 2024-05-24 广州巴宝莉化妆品有限公司 一种鲜花花香提取物的持久留香的方法
CN118059005B (en) * 2024-04-22 2024-07-09 广州巴宝莉化妆品有限公司 Method for keeping fragrance of fresh flower fragrance extract

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