WO2021082537A1

WO2021082537A1 - Crop phenotype high-throughput acquisition apparatus and climate chamber

Info

Publication number: WO2021082537A1
Application number: PCT/CN2020/102887
Authority: WO
Inventors: 傅秀清; 姜东�; 丁艳锋; 吴劼; 周国栋; 毛江美
Original assignee: 南京农业大学
Priority date: 2019-10-28
Filing date: 2020-07-19
Publication date: 2021-05-06
Also published as: CN110612843A

Abstract

A crop phenotype high-throughput acquisition apparatus. The interior of the acquisition apparatus is provided with phenotype acquisition units for respectively acquiring the phenotype characteristics of a crop at two view angles. Root boxes provided in the middles of the phenotype acquisition units are arranged on a working plane in a straight line along a first movement direction of a first phenotype acquisition unit; the side surface, parallel to the straight line, of the surface of each root box is configured to be transparent so as to photograph an underground phenotype characteristic. In order to obtained recombined underground phenotype characteristic information, the two sides of a head surface in the root box further can be close to each other, the root box is configured to be a flat cuboid structure, and the growth space of a crop root system is compressed so as to be close to a transparent side wall, and thus can be photographed by the phenotype acquisition unit. Further disclosed is a climate chamber provided with the foregoing acquisition apparatus.

Description

Crop phenotype high flux acquisition device and climate cabin

Technical field

The invention relates to the technical field of crop phenotype acquisition and analysis, in particular to a crop phenotype high-throughput acquisition device and a climate chamber.

Background technique

Increasing crop yields is the most effective way to ensure food security. Crop breeding is an effective means to increase crop yield. The development of functional genomics and genetic technology in the breeding field is the most convenient and effective means to increase grain production.

The phenotype of crops is the external expression of crop genes, and is the result of the interaction of crops' own genes and the external environment. Therefore, it is particularly important to explore the relationship between crop genotypes, environmental factors, and crop phenotypic characteristics and traits.

The traditional artificial climate chamber is generally used to monitor and control the growth environment of crops. It has certain environmental regulation functions and can be applied to experiments such as crop genetic improvement and new species cultivation, avoiding many unstable constraints in the natural environment. Improve the work efficiency of plant genome function research and molecular breeding.

In order to cultivate good crop varieties, it is necessary to continuously measure the changes in the phenotypic characteristics and physiological parameters caused by the growth of the organs during the growth of the crops. At present, the traditional artificial climate chamber only has the function of cultivating crops, and the measurement of the phenotype of the crops mainly relies on manual observation and measurement to describe and record the external characteristics of the crops. Because this work relies on manual detection of individual traits of small samples of plants, the amount of data is limited, the efficiency is low, it is difficult to carry out comprehensive analysis of multiple traits of plants, and it is extremely easy to introduce errors in the measurement data due to the introduction of human measurement errors. The resulting relationship between genotype, environmental factors and crop phenotype is not accurate.

Summary of the invention

In response to the needs of plant genomics research and molecular breeding and the deficiencies of existing phenotype acquisition technologies, the present invention provides a crop phenotype high-throughput acquisition device and climate chamber. The present invention can achieve high throughput, high precision and low cost. The phenotype acquisition and analysis device meets the needs of acquiring phenotypic data related to plant growth, yield, quality, and tolerance to biotic and abiotic stresses. The present invention specifically adopts the following technical solutions.

First of all, in order to achieve the above objective, a high-throughput acquisition device for crop phenotypes is proposed, which is set in the climate chamber and includes: root boxes arranged in a straight line along the first direction on the working plane. The root boxes are respectively set in a flat rectangular parallelepiped structure with transparent two sides, the spacing between the two transparent sides in the root box is in the range of 10mm-20mm, and the two transparent sides in each root box are parallel to the first One direction; the first phenotype acquisition unit, including two set on the plane where the root box is located, the two first phenotype acquisition units are respectively set on both sides of the root box parallel to the first direction, each The first phenotype acquisition unit is respectively used to acquire phenotype information of the crops contained in the root box in a side view corresponding to the two transparent sides in the root box; the second phenotype acquisition unit is set where the root box is located The upper part of the plane is used to obtain the phenotype information of the crops contained in the root box in the top view.

Optionally, the above-mentioned crop phenotype high-throughput acquisition device, wherein the root box includes: a root box skeleton, which has a plurality of uprights, and a bottom plate connected to the bottom end of each of the uprights, the uprights and the bottom plate A root accommodating space formed in a flat rectangular parallelepiped structure for accommodating the roots of the crops, the edge of the bottom plate is also provided with a card slot; side baffles, which are arranged on the left and right sides of the root accommodating space, and are fixedly connected to the upright column , The bottom of the side baffle is tightly connected to the edge of the bottom plate; the light-transmitting plate is arranged on the front and back sides of the root accommodating space, and is plug-connected to the upright column, and the side baffle is Transparent material, the bottom of the side baffle is clamped into the slot on the edge of the bottom plate, the light-transmitting plate, the column and the side baffle close the root accommodating space; in each of the root boxes, the front The distance between the light-transmitting plates on the rear two sides is in the range of 10mm-20mm, and the light-transmitting plates of each root box are arranged in a straight line along the first direction on the working plane; the light-shielding plate is close to the The light-transmitting plate is arranged on the outer side of each of the light-transmitting plates, and is detachably connected to each of the uprights in the root accommodating space; an upper end cover of the root box, which is fixedly connected to the upper end of each of the uprights, and the upper end of the root box A through hole for accommodating crop growth is left in the middle of the cover.

Optionally, in the above-mentioned crop phenotype high-throughput acquisition device, each of the first phenotype acquisition units includes: a first-direction sliding guide rail, which is parallel to the transparent side surface of the root box of the crop and runs along the first direction The sliding plate is arranged on the outside of the root box; the sliding plate is arranged on the sliding guide rail in the first direction, and the sliding guide rail is translated in the first direction along the first direction; the sliding guide rail in the second direction is connected with the lower end of the sliding guide rail in the first direction. The sliding plate is fixedly connected, the second direction sliding guide rail is kept perpendicular to the upper surface of the sliding plate, and is arranged along the second direction; the third direction sliding guide rail, which is connected with the second direction sliding guide rail, faces the root of the crop The box is arranged in the third direction; an image acquisition device is arranged on the end of the third-direction sliding guide toward the root box, and is used to collect the root box and/or the crops contained in the root box in the corresponding The transparent two sides of the root box in the side view of the image; a background board, which is set on one side of the image capture device; the third-direction sliding guide rail in the second direction slides the guide rail in the second direction When moving, the image acquisition device is driven to move synchronously, and the height of the image acquisition device relative to the root box and/or the crop contained in the root box is adjusted; the third-direction sliding guide rail is relative to the second direction When the sliding guide rail moves in the third direction, the image acquisition device is driven to move synchronously, and the distance of the image acquisition device relative to the root box and/or the crop contained in the root box is adjusted.

Optionally, in the above-mentioned crop phenotype high-throughput acquisition device, the second phenotype acquisition unit includes: a top sliding guide rail, which includes a sliding guide rail parallel to the first direction and fixed in the first direction respectively along the first direction. The two top sliding guides above the root box of the crop, the two top sliding guides are respectively arranged on both sides of the root box of the crop; the middle sliding guide, the two ends of which are respectively connected to the two top sliding guides, the middle The sliding guide rail translates along the first direction on the lower side of the top sliding guide rail; the lower sliding guide rail, the upper end of which is connected with the middle sliding guide rail, the lower end of which is fixed with a top view image acquisition device, and the lower sliding guide rail is perpendicular to The middle sliding guide rail and the top sliding guide rail move relative to the middle sliding guide rail in a second direction; the top view image capture device is fixed on the lower sliding guide rail downward toward the top of the root box At the bottom, the top-view image acquisition device is used to collect images of the root box and/or the crop contained in the root box in the top-view perspective.

At the same time, in order to achieve the above object, the present invention also provides a climate cabin, which includes: a crop cultivation area, the inner periphery of which is provided with: ground exhaust ducts, side exhaust ducts, top air return ducts, the ground exhaust ducts And/or the side exhaust pipe is connected to the output end of the air mixing valve, and the input end of the air mixing valve is connected with an air conditioning device, a carbon dioxide supply device, an ozone supply device, and a humidification device. The top air return pipe connects the crop The gas in the cultivation area is sent back to the air-conditioning device and then supplied to the crop cultivation area by the air-conditioning device through the ground exhaust pipe and/or the side exhaust pipe. The phenotype acquisition area is equipped with an electric sliding door, so One side of the electric sliding door is connected to the phenotype acquisition area, the other side of the electric sliding door is connected to the crop cultivation area, and the phenotype acquisition area is provided with optional, the above-mentioned crop phenotype is high Flux acquisition device; environmental equipment area IV, used for fixed installation of the air-conditioning device, carbon dioxide supply device, ozone supply device, and humidification device.

Optionally, the above-mentioned climate cabin, wherein the ground exhaust pipe, the side exhaust pipe, and the top return air pipe extend from the crop cultivation area and are connected to the phenotype acquisition area, the crop cultivation area and An environmental sensor group is also provided in the phenotype acquisition area, and the environmental sensor group is used to collect environmental data in the crop cultivation area and the phenotype acquisition area to adjust the air conditioning device, carbon dioxide supply device, and ozone supply. The working status of the device and the humidifying device.

Optionally, in the climate cabin described above, wherein the crop cultivation area is further provided with a crop cultivation rack, the crop cultivation rack is arranged in a multi-layer structure, and each layer of the crop cultivation rack is arranged with each other. A plurality of root box racks in parallel; partitions are respectively arranged between the top layer of the crop cultivation rack and each layer structure, and spray nozzles are arranged in the middle of the partitions for spraying moisture and nutrients to the crops below, so A plurality of fill light lamps are evenly arranged in the length direction of the partition to provide light. The upper surface of the partition is set to be convex around and flat in the middle, and the upper surface of the partition is used to collect the upper surface. A layer of sprayed moisture and nutrients; the root box frame is a long plate structure, and a plurality of root box installation grooves are provided along the length direction of the long plate structure, and each root box passes through each of the root box installation grooves. , The lower edge of the upper end cover of the root box abuts against the upper surface of the root box installation groove, each of the root boxes is fixed under the long plate structure, and both ends of the root box rack are respectively provided with handles, A groove structure is provided under the handle, and the groove structure is clamped and fixed to the crop cultivation frame, and each of the root boxes is erected between the partitions of the crop cultivation frame.

Optionally, the above climate cabin, wherein the environmental sensor group includes a light sensor, a humidity sensor, a temperature sensor, a CO2 sensor, and the environmental sensor group is installed on the side of each shelf in the crop cultivation rack, near the root box Set the growing area of the inner crop.

Optionally, in the above-mentioned climate cabin, the crop cultivation area, the phenotype acquisition area and the environmental equipment area are arranged in the same container, and are enclosed by the container.

Beneficial effect

In the present invention, a phenotype acquisition unit is provided in the climate chamber to acquire the phenotype characteristics of the crop in two viewing angles. One of them is set on both sides of the root box to obtain the phenotype information of the crops contained in the root box in a side view corresponding to the transparent two sides of the root box; the other can be set above the root box , Used to obtain the phenotypic information of the crop from the top view. The root box, in order to facilitate the phenotype acquisition unit of the above-mentioned crop phenotype characteristics to take its image to extract the phenotype characteristics, the root box can generally be arranged on the working plane along the first direction of the movement of the first phenotype acquisition unit as A straight line, and the side surface of the root box parallel to the straight line is set to be transparent for photographing the surface features of the ground. In order to obtain the reorganized ground surface typographic feature information, the two sides of the head surface in the root box can also be close to each other. The root box is set in a flat rectangular parallelepiped structure to compress the growth space of the crop root system and make it close to the transparent side wall. Shoot.

Further, the root boxes can be connected and fixed by a root box frame, and the root box frame is fixed on the crop cultivation frame or moved from the crop cultivation frame to the root box fixing frame on the working plane to collect phenotypic characteristics. Among them, in order to protect the root system from being affected by external light conditions during the cultivation process of the crops, a light-shielding plate can be further provided on the outside of the light-transmitting plate in the root box. Specifically, the shading plate can be detachably connected with the support structure through the magnetic attraction of the magnetic strip. When obtaining the crop phenotype, fix the root box holder on the root box holder through the groove structure on the lower surface of the root box, and remove the light-shielding plate. In this way, the light-transmitting plates on both sides of the root box can be directly Shows the ground surface typographic characteristics of the crop. In this way, the image acquisition device can be directly translated from both sides of the root box frame along a straight line, and the ground surface features of the crop can be directly collected.

In the present invention, the two first phenotype acquisition units are respectively arranged in parallel and opposite to each other along the two sides of the root box of the crop, and the two first phenotype acquisition units operate synchronously. In the two first phenotype acquisition units, the image acquisition device keeps facing the background board in the other first phenotype acquisition unit on the opposite side, and the image acquisition device and the background board move synchronously to ensure that the image acquisition device captures When crop phenotype images, the background panel can always block the environmental image behind the crop, which facilitates the later extraction of crop phenotypic features in the image through the image processing system. Therefore, in the present invention, the image acquisition device in the phenotype acquisition unit can scan each root box arranged in the first direction and the crops in the root box one by one along the first direction, and sequentially obtain the table of each crop in each root box. Type information. The invention can acquire multiple sets of crop top-down graph data in real time, at a fixed time and at a fixed point, and then complete the storage, transmission and phenotypic data analysis of multiple sets of crop top-down graph data.

Other features and advantages of the present invention will be described in the following description, and partly become obvious from the description, or understood by implementing the present invention.

Description of the drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and together with the embodiments of the present invention, are used to explain the present invention, and do not constitute a limitation to the present invention. In the attached picture:

Figure 1 is a schematic diagram of the overall structure of the climate cabin of the present invention;

Figure 2 is an exploded view of the climate chamber of the present invention;

Figure 3 is a schematic diagram of the overall structure of the crop cultivation rack of the present invention;

Fig. 4 is a partial schematic diagram of the crop cultivation rack in Fig. 3;

5 is a schematic diagram of the partition structure in the crop cultivation rack of FIG. 3;

Fig. 6 is a schematic structural diagram of a root box rack provided in the crop cultivation rack of Fig. 3;

7 is a schematic diagram of the assembly process of the root box set in the root box rack of FIG. 3;

8 is a schematic diagram of the overall structure of the first phenotype acquisition unit in the climate chamber of the present invention;

FIG. 9 is a top view of the first phenotype acquisition unit in FIG. 8;

10 is a schematic diagram of the installation method of the first phenotype acquisition unit and the second phenotype acquisition unit in the climate chamber of the present invention;

Figure 11 shows the flash structure at the bottom of the open slot.

In the figure, 1 represents the sliding guide rail in the first direction; 11 represents the cover of the root box; 12 represents the upper end cover of the root box; 13 represents the frame of the root box; 14 represents the light-transmitting plate; 15 represents the magnetic strip; 2 represents the other first direction sliding Guide rail; 3 means image acquisition equipment; 31 means sliding board; 32 means sliding guide rail in the second direction; 33 means sliding guide rail in third direction; 4 means background plate; 41 means knurled structure of light shield; 42 means side baffle; 5 means Top view image acquisition equipment; 51 represents the lower sliding rail; 52 represents the middle sliding rail; 53 represents the top sliding rail; 6 represents the partition; 61 represents the fill light; 62 represents the nozzle; 63 represents the connector; 7 represents the root box rack; 71 represents the handle; 72 represents the groove structure; 8 represents the crop cultivation rack; 81 represents the fixed rod; 82 represents the L-shaped plate, 83 represents the load cell; 9 represents the root box fixed rack; 21 represents the ground exhaust duct; 22 represents the side Exhaust duct; 23 represents the top return duct.

Detailed ways

In order to make the objectives and technical solutions of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be described clearly and completely in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

Those skilled in the art can understand that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meanings as those commonly understood by those of ordinary skill in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with the meanings in the context of the prior art, and unless defined as here, they will not be used in idealized or overly formal meanings. Explanation.

In the present invention, the meaning of "and/or" means that each exists alone or both exist simultaneously.

The meaning of "inside and outside" in the present invention refers to the root box itself, the direction pointing to the root system of the crop contained in the root box is inside, and vice versa; it does not refer to the device mechanism of the present invention. The specific limit.

The meaning of "left and right" in the present invention means that when the user is facing the direction of the light-transmitting plate, the user's left is the left, and the user's right is the right, instead of the device mechanism of the present invention. Specific restrictions.

The meaning of "up and down" in the present invention means that when the user is facing the work plane on which the root box is placed, the direction from the work plane to the crop is up, and vice versa, it is down, not for the present invention. The specific limitation of the device mechanism.

The meaning of "connection" in the present invention can be a direct connection between components or an indirect connection between components through other components.

Fig. 1 is a climate chamber for high-throughput, high-precision and low-cost crop phenotype acquisition and analysis according to the present invention. It has the following characteristics: (1) Control crop cultivation light, humidity, temperature, CO ₂ concentration, O ₃ concentration and other influencing factors for crop cultivation, (2) Acquire and analyze above-ground phenotypes, (3) Realize above-ground phenotypes Obtain, (4) Moveable. This kind of climate chamber can solve the problem that the existing climate chamber cannot carry out accurate and automatic acquisition and analysis of crop phenotypes, and it is also convenient for truck transportation due to the use of an external structure similar to a container.

The climate cabin can be specifically set up as shown in Figure 1 or Figure 2, including:

Control and display area I, crop cultivation area II, phenotype acquisition area III, environmental equipment area IV. The climate cabin can be set in the container, and the front door for the operator to enter and exit the climate cabin is set on the container, and the touch display can be used for the operators to operate outside the cabin and observe the conditions in the cabin. The climate cabin can be installed with various equipment Body, an exhaust fan that can lower the temperature of the environmental equipment area, and a side door for operators to enter and exit the environmental equipment area. Inside the container:

Control and display area I, used to install the climate cabin control and analysis system, can set up _{the control unit for lighting, humidity, temperature, CO 2} concentration, O ₃ concentration and other environmental factors to realize the controllable environmental conditions in the cabin and connect to the interior of the climate cabin The environmental sensor group can realize the real-time collection of data of environmental factors such as light, humidity, temperature, CO ₂ etc., and can carry out numerical control programming to control the phenotype acquisition device in the phenotype acquisition area III to realize the collection of crop phenotype data, which can develop the environment Comprehensive analysis of phenotypic data under the influence of conditions, real-time observation of the status of crop cultivation area II and phenotype acquisition area III; wall-mounted air conditioners can also be installed in the control and display area I to provide comfortable work for operators Environment and provide a suitable operating environment for the climate cabin control and analysis system equipment;

The crop cultivation area II is provided with an electric sliding door connected to the control and display area I and the phenotype acquisition area III. One of the electric sliding doors is made of transparent glass material and is installed between the control and analysis area I and the crop cultivation area II, which is convenient for personnel to enter and exit the crop cultivation area II and observe the conditions in the crop cultivation area II in the control and analysis area I ；

The electric sliding door of the phenotype acquisition area III is made of opaque material and is installed between the crop cultivation area II and the phenotype acquisition area III to facilitate the entry and exit of the phenotype acquisition area III. After the door is closed, the crop phenotype acquisition can be guaranteed When there is no other ambient light in the entire phenotype acquisition area III that affects the normal operation of the top view graph acquisition sensor group and the side view phenotype acquisition sensor group, it is convenient for the processing and analysis of the phenotype data. The phenotype acquisition sensor group can be realized by image acquisition equipment such as a camera.

The crop cultivation area II is provided with the crop cultivation rack 8 shown in FIG. 3, which includes: a partition, a root box, a root box rack, a nutrient solution tank with a pump, a water pipe, a nozzle, a light supplement, and an environmental sensor Group structure. Among them, the nutrient solution tank with pump is installed at the bottom of the crop cultivation rack, and the climate chamber control and analysis system controls the nutrient solution contained in the nutrient solution tank with pump to transport the nutrient solution to the crop cultivation through the water pipe in the crop cultivation rack 8. In each layer of the shelf, the spray nozzle in the shelf sprays the crops under the shelf.

The sprinkler head and the light supplement lamp are installed under the partition and can be used to provide nutrient solution and light necessary for the growth of crops. The above-mentioned crop cultivation area II and phenotype acquisition area III can be uniformly set as a climate chamber controlled by the environmental equipment area IV, which has a climate chamber control and analysis system, which can adjust the amount of nutrient solution sprayed by the spray nozzle in real time according to the needs of crop cultivation And the light intensity of the fill light.

In the crop cultivation rack 8 of the crop cultivation area II, the partition can adopt the structure shown in FIG. 5, and be installed between the layers of the crop cultivation rack in the manner shown in FIG. 4, and can be installed according to the structure of the crop cultivation rack. For quantity requirements, install multi-layer partitions, and each partition can be set to a structure with a convex surface on the upper surface and a concave flat in the middle, which is convenient for collecting the excess nutrient solution sprayed by the spray nozzle in the upper crop growth area. An environmental sensor group can be further installed on the side of each shelf in the crop cultivation rack. The environmental sensor group includes a light sensor, a humidity sensor, a temperature sensor, and a CO ₂ sensor. It is set close to the growing area of the crop in the root box, which can be accurate Real-time collection of environmental parameters of crop growing areas.

As shown in FIG. 6, both ends of the root box rack 7 are respectively provided with handles 71, and a groove structure 72 in the root box rack 7 shown in FIG. 4 is provided below the handle 71 to fix the root box架7. For the crop cultivation rack 8 shown in FIG. 4, the root box rack 7 is arranged on the crop cultivation rack 8, and the material cultivation rack 8 is provided with a fixed rod 81, and the fixed rod is clamped into the The groove structure 72 under the root box rack 7 supports and fixes each of the root boxes. As for the phenotype acquisition area III shown in FIG. 2, when the phenotype of the crop is acquired, the root box rack 7 is removed from the crop cultivation rack 8 and placed on the root box fixing rack 9. The upper end of the bracket is clamped with the groove structure 72 to fix the root box, prevent the root box frame from shaking back and forth and support the root box frame. During the sampling process, the light-shielding plate of each root box separates from the magnetic strip 15 and the sampling device photographs the root structure of the crop inside the root box through the light-transmitting plate 14 of each root box. The root box fixing frame 9 can be referred to as shown in FIG. 9 or FIG. 10, which is installed on the upper surface of the seedbed by fastening bolts for placing the root box frame. The root box fixing frame 9 can be set to the corresponding interval according to the data requirements of obtaining the crop top view chart type, and multiple root box fixing frames are installed to support the multiple root box frames, and the system and the side view chart are obtained through the top view chart type. The type acquisition system separately acquires the multi-angle chart type data of multiple groups of crops.

An L-shaped plate 82 connecting the vertical pillars of the crop growing frame 8 shown in FIG. 4 is arranged below the fixed rod 81 of the crop growing frame 8. One side of the L-shaped plate 82 is connected to the upper surface of the other side of the vertical pillar. Set the weighing sensor 83. The root box frame 7 is installed on the upper part of the load cell by screw connection, and is used to support the root box. The fixed rod 81 set above the load cell can be equipped with multiple root box racks 7 according to the requirements of the number of cultivated crops. The load cell is installed on the L-shaped plate by screws, and is used to monitor the weight change of the root box frame 7 in real time, obtain the supply amount of nutrient solution according to the weight change, and transmit it to the control and analysis system for corresponding data recording. The L-shaped plate is installed on the crop cultivation rack by screws, and is used to support the load cell, the root box and the root box rack.

In a more specific implementation manner, the root box is composed of an upper end cover of the root box, a root box skeleton, a light-transmitting plate, a light-shielding plate, a side baffle, a magnetic strip, and screws as shown in FIG. 7. The root box is placed in the rectangular hole of the root box frame, and the outer size of the upper end cap of the root box is larger than the shape size of the rectangular hole on the root box frame, which is convenient for the root box to be placed on the upper surface of the root box frame; the root box can provide the necessary crop growth The hydroponic and soil culture environment, its rectangular shape and the characteristics of light transmission inside and shading outside are convenient for the cultivation of roots, stems and leaves of crops and the extraction of phenotypes.

In order to be able to obtain more characteristic information in the bottom surface of the crop, the width of the side baffle 42 is set to not exceed the range of 10-20 mm, and the width of the light-transmitting plate 14 exceeds 10 mm. The root box containing the thickness of the root system of the crop is compressed to 10 mm, and the growth of the root system is restricted on the front and back sides of the crop, and the root system of the crop grows close to the light-transmitting plate, so that it can be easily acquired by general image acquisition equipment.

The root box skeleton can be made by 3D printing technology. There are two card slots on the inner side of each frame, a total of eight card slots. The upper surface of the bottom can also have four additional slots to facilitate the installation and removal of the light-transmitting plate and the lower edge of the side baffle. There can be two card slots on the outer side of the front of each frame, four card slots in total, which are used for interference fit with the magnetic stripe.

Wherein, the grooves on the uprights on the front and back sides of the root accommodating space correspond to first sliding grooves parallel to the axis of the upright, and the edges on both sides of the light-transmitting plate 14 are respectively inserted into two adjacent ones. Root the first chute on the surface of the column, the light-transmitting plate 14 moves downward along the first chute to abut against the front edge or the rear edge of the bottom plate. The light-transmitting plate and the side baffle plate are installed on the root box skeleton along the inner groove of each frame of the root box skeleton and the groove on the upper surface of the bottom. The phenotype of the root system can be obtained through the light-transmitting plate.

The grooves on the front and back sides of the vertical column of the root accommodating space correspond to a first mounting groove parallel to the axis of the vertical column, and a magnetic strip 15 is provided in the first mounting groove. The bar 15 is snapped into the first installation slot with interference.

At least the edge of the light-shielding plate is provided with a magnetically conductive material, or the whole can be made of iron sheet or other opaque magnetically conductive material. The magnetically conductive material is attracted by the magnetic strip 15 and fixed on the surface of the column. The said magnetic strips can be arranged in four of the outer card slots on the front of each frame respectively, and the light-shielding plate is attracted to the magnetic strips by magnetic force to realize the light-shielding effect.

In order to facilitate the fixing of the root box on the root box rack to realize unified transportation and fixation, the outer edge of the upper end cover 12 of the root box protrudes from the plane where the light shielding plate is located, and is fixed on the upper end surface of the upright column by screws. The root box is placed in the rectangular hole of the root box rack, and the outer size of the upper end cap of the root box is larger than the outer size of the rectangular hole on the root box rack, which is convenient for the root box to be placed on the upper surface of the root box rack; the root box can provide crops The hydroponic and soil culture environment is necessary for growth, and the rectangular shape and the characteristics of light transmission and shading facilitate the cultivation of the roots, stems and leaves of crops and the extraction of phenotypes. The through hole in the middle of the upper end cover 12 of the root box can be specifically configured as a rectangle, and a root box cover plate 11 is embedded in the rectangular through hole, and a center hole or a tapered hole is provided in the middle of the root box cover plate 11 , For accommodating crop growth.

During cultivation, the seeds of the crop or seeds with germinated roots can be placed in the tapered hole of the root box cover 11. The tapered hole can fix the relative position of the crop. By fixing the position of the crop, it is convenient for the appearance of the crop. Type of automatic acquisition;

The root box cover 11 is installed in the opening groove of the upper end cover of the root box through a number of raised structures on the side, and several raised structures on the side can ensure that the root box cover 11 is stuck in the opening groove of the upper end cover of the root box. Figure 11 , The flash structure at the bottom of the opening groove of the upper end cover of the root box can ensure that the root box cover 11 will not be pressed into the root box due to excessive external force during the installation of the root box cover 11;

The light-shielding plate can also be selected to be inserted and installed on the upright post of the root box frame along the inner side of each frame of the root box frame and the snap groove on the bottom upper surface, so as to block light transmission.

When the root box is placed in the crop cultivation area, the shading plate is in the installed state, which is convenient to block the ambient light and reduce the impact of ambient light on the crop root system; when the root box is placed in the phenotype acquisition area, the shading plate can be sucked or pulled out with a magnet The card slot is set to facilitate the acquisition of the root phenotype.

When the root box is placed in the crop cultivation area, the shading plate is in the installed state, which is convenient to block the ambient light and reduce the impact of ambient light on the crop root system; when the root box is placed in the phenotype acquisition area, the shading plate can be taken out to facilitate the root system table Type of acquisition;

A knurled structure 42 can also be provided on the surface of the shading plate, which is convenient for the operator to install and disassemble the shading plate according to usage requirements.

In the above-mentioned phenotype acquisition area III, the root box is erected on the root box fixing frame 9 in the middle of the working plane through the root box frame 7. A high-throughput photographing system for acquiring the phenotype of the crop is set on both sides and above the root box in the working plane to photograph the phenotype information of the crop. The system includes: a first phenotype acquisition unit arranged on the working plane, and a second phenotype acquisition unit arranged above the working plane, which are respectively used to acquire the phenotype characteristics under the horizontal view of the crop and the phenotype characteristics under the overhead view. .

The root boxes with cultivated crops are arranged in a straight line along the first direction and placed on the working plane as shown in Fig. 9 or Fig. 10. At least two sides of the root box along the first direction can be set to be transparent, so as to facilitate the acquisition of images of the underground part of the crop inside the root box, and extract the ground and subsurface characteristics of the corresponding crop from the image. In order to facilitate the arrangement of the root boxes, they can be arranged in a long root box frame 91 fixed by the root box fixing frame 9 on the working plane. Among them, the top of the root box is provided with a flash structure beyond the edge of the main structure, and the root box frame is provided with a through slot corresponding to the size of the main structure of the root box. When the root box is installed, the main structure of the root box is nested in the through groove on the root box frame, and the flash structure abuts on the upper surface of the through groove to realize the fixation of the root box. The working plane can be set as a seedbed capable of accommodating the root box and the corresponding phenotype acquisition unit. In some implementations, the root box fixing frame can be installed on the upper surface of the seedbed by fastening bolts, and a root box frame 91 is placed on the root box frame 91. Each root box is arranged along a straight line in the root box frame 91, and the root box is in the root box. The sides of the frame 91 in the length direction are set to be transparent for photographing ground phenotypes, or, when the root box is opaque, it can be used to cultivate and fix crops for photographing ground phenotypes. The spacing between the root boxes needs to be set according to the data requirements for obtaining the crop top view graph type, so as to ensure that the crops will not overlap and cause interference during the top view shooting.

In an implementation manner, referring to FIG. 8, the above-mentioned first phenotype acquiring unit includes:

The sliding guide rail 1 in the first direction is parallel to a side surface of the root box of the crop and is arranged along the first direction;

The sliding plate 31 is arranged on the sliding guide rail 1 in the first direction, and the sliding guide rail 1 is translated in the first direction along the first direction;

The second-direction sliding guide 32 has a lower end fixedly connected to the sliding plate 31, and the second-direction sliding guide 32 is perpendicular to the upper surface of the sliding plate 31 and is arranged along the second direction;

The third-direction sliding guide 33 is connected to the second-direction sliding guide 32, and the root box facing the crop is set in the third direction;

An image acquisition device 3, which is disposed at an end of the third-direction sliding guide 33 facing the root box, and is used to acquire an image of the root box and/or the crop contained in the root box in a first angle of view;

The background board 4 is arranged on one side of the image acquisition device 3;

When the third-direction sliding guide 33 moves in the second direction along the second-direction sliding guide 32, it drives the image acquisition device 3 to move synchronously, and adjusts the image acquisition device 3 relative to the root box and/ Or the height of the crops contained in the root box; when the third-direction sliding guide 33 moves in the third direction relative to the second-direction sliding guide 32, the image acquisition device 3 is driven to move synchronously to adjust the The distance of the image acquisition device 3 relative to the root box and/or the crop contained in the root box.

In order to cooperate with the root box, in a more preferred manner, the root boxes are arranged in a row along the first direction, and the image capture device 3 slides the guide rail 1 along the first direction along with the slide plate 31 in the first direction. Shift in the first direction, and sequentially take images of each of the root boxes and/or the crops contained in the root boxes.

Considering that there is a certain difference in phenotype information on both sides of the crop, in order to obtain more comprehensive phenotypic characteristics, the first phenotype acquisition unit can be set to two, which are respectively arranged on both sides of the root box parallel to the root box rack. . Specifically, the first direction sliding guide rails 1 in the two first phenotype acquisition units are respectively arranged on both sides of the root box of the crop parallel to the first direction, and are fixedly arranged on the same root box as the root box. On the same working plane. The third-direction sliding guide rail 33 in each of the first phenotype acquisition units and the image acquisition device 3 and the background board 4 at the end of each third-direction sliding guide 33 are respectively arranged opposite to each other; The first phenotype acquisition unit separately captures images of different sides of the root boxes and/or crops contained in the root boxes under the horizontal viewing angle.

Wherein, in order to avoid errors and extra calculations caused by the contralateral phenotype acquisition unit and the environmental background to the crop phenotype extraction, the above-mentioned first phenotype acquisition unit is also respectively provided with a background board, which is used as a background when photographing crops. At this time, referring to FIG. 8 or FIG. 9, the slide plates 31 in the two first phenotype acquisition units are driven to move synchronously along the first direction, and any one of the first phenotype acquisition units The image capture device 3 in the image acquisition device always keeps facing the background plate 4 in the first phenotype acquisition unit on the opposite side.

In order to obtain the phenotypic characteristics of the crop crown or the whole branches and leaves, it is generally necessary to photograph the crop from top to bottom to extract the corresponding characteristics. This viewing angle requires a second phenotype acquisition unit. In an implementation manner, the second phenotype acquisition unit includes:

The top sliding guide rail 53 includes two sliding guide rails 1 parallel to the first direction and fixed above the root box of the crop in the first direction. The two top sliding guide rails 53 are respectively arranged on the top of the crop. Both sides of the root box;

The middle sliding guide rail 52 is connected to two top sliding guide rails 53 at both ends, and the middle sliding guide rail 52 is translated in the first direction on the lower side of the top sliding guide rail 53;

The upper end of the lower sliding guide rail 51 is connected with the middle sliding guide rail 52, and the second viewing angle image acquisition device 5 is fixed at the lower end. The lower sliding guide rail 51 is perpendicular to the middle sliding guide rail 52 and the top sliding guide rail 53, Move relative to the middle sliding guide 52 in the second direction;

The second-view image acquisition device 5 is fixed on the lower end of the lower sliding guide 51 toward the top of the root box downward, and the second-view image acquisition device 5 is used to collect the root box and/or The image of the crop contained in the root box in the second perspective.

In a more specific implementation manner, the above-mentioned first direction, third direction, and second direction may respectively correspond to the three directions of XYZ. The X-Y plane forms the working plane, or seedbed plane.

Therefore, in the present invention, the corresponding side view phenotype acquisition sensor group can be installed through the first phenotype acquisition unit to acquire crop phenotype data under the side view angle. The side view phenotype acquisition sensor group is installed on the corresponding Y-direction sliding guide rail in the first phenotype acquisition unit. In some implementations, the side view phenotype acquisition sensor group may specifically include a visible light sensor, a multispectral sensor, a hyperspectral sensor, a thermal imaging sensor, a lidar sensor, and the like. The side view phenotype acquisition sensor group can be driven by a servo motor capable of outputting an X-direction driving force and an X-direction sliding guide corresponding to the servo motor, and the side view phenotype acquisition sensor group can be translated along the crop root box frame, thereby achieving the acquisition of the side view phenotype. In the sensor group, the focal length of each sensor is adjustable.

The background board is installed on the Y-direction sliding guide rail of the side view phenotype acquisition system, and the background board is driven by the Y-direction servo motor and the Y-direction sliding guide rail. The above-mentioned overall high-throughput camera system can be set in the environment of the climate cabin. The climate chamber environment is adjusted and recorded in accordance with the set requirements to correspond to various phenotype data and provide a data basis for the study of the relationship between phenotype and environment. The climate cabin is equipped with a control and analysis system, which can simultaneously control two sets of side view phenotype acquisition systems to cooperate with each other: when the side view phenotype acquisition system and the side view phenotype acquisition sensor group of the side view phenotype acquisition system begin to acquire phenotype data, The side view phenotype acquisition system on the other side needs to drive the background plate set on it to move to the position corresponding to the side view phenotype acquisition sensor group. During the imaging process of the side view phenotype acquisition sensor group, the single color A rectangular background plate is used as a background to obtain phenotype data on one side of crop stems, leaves and roots. The setting of the background board is conducive to the processing and analysis of the later phenotypic data. The two sets of side view phenotype acquisition systems controlled by the climate cabin control and analysis system cooperate with each other to complete the acquisition of phenotype data on the two sides of crop stems, leaves and roots.

In order to ensure the image collection effect, in the above-mentioned climate cabin, a lighting system can be further provided on the top of the working platform. The climate cabin control and analysis system can control the lighting system to be turned on when shooting, and to turn off when the shooting ends to reduce the impact of external light on the bare crop roots according to the needs of use.

In order to obtain crop phenotype data from a top-down perspective, the above-mentioned second phenotype acquisition unit may be specifically configured as a top-down graph-type acquisition system installed on the top of the climate chamber box. It includes a servo motor in three directions of XYZ, a sliding guide rail in three directions of XYZ, and a second-view image acquisition device 5 composed of a top-view graph-type acquisition sensor group. The climate cabin control and analysis system can control the XYZ three-direction servo motors to drive the XYZ three-direction sliding guides in real time according to the requirements of the top-down graph data acquisition of crop stems and leaves, so as to drive the top-down graph acquisition sensor group to achieve the right Obtaining multiple sets of crop top view graph data. The climate cabin control and analysis system can control the top view graph acquisition sensor group to obtain multiple sets of crop top view graph data in real time, timing and fixed point, and then complete the storage, transmission and phenotypic data analysis of multiple sets of crop top graph data.

The top-view graph acquisition sensor group is installed at the lower end of the Z-direction sliding guide rail of the top-view graph acquisition system, and it can be set to include visible light sensors, multispectral sensors, hyperspectral sensors, thermal imaging sensors, lidar sensors, etc. sensor. The above-mentioned top-view graph acquisition sensor group is driven by the Z-direction servo motor to move synchronously with the Z-direction sliding guide rail, so as to realize the photographing of crop phenotypes at different positions under the top view angle. The focal length of each sensor in the top-view graph acquisition sensor group is adjustable, which is convenient for automatic phenotype acquisition.

The above-mentioned first phenotype acquisition unit and the second phenotype acquisition unit are also respectively provided with corresponding to the first direction (for example, the X-axis direction), the second direction (for example, the Y-axis direction), and the The drive device in the third direction (for example, the Z-axis direction), each of the drive devices includes three-direction servo motors and transmission components respectively connected to the drive shafts of the servo motors. The transmission component correspondingly drives the connecting structure of the sliding guide rails in three directions to move, so as to realize the adjustment of the position of the side view phenotype acquisition sensor group, the background board or the top view diagram type acquisition sensor group. In a typical implementation manner, the transmission assembly can drive the image acquisition device through a servo motor and a ball screw nut pair connected to the drive shaft of the servo motor. The image acquisition devices of each viewing angle are respectively connected to the ball screw nut pair corresponding to the direction in which they are located. The servo motor drives the ball screw nut pair to move in the first direction, the second direction, or the third direction to drive the image of the corresponding viewing angle. The acquisition device moves synchronously with the ball screw nut pair in the direction of the change.

In order to ensure that the two first phenotype acquisition units can move synchronously, and ensure that the background board can be set on the opposite side of the camera to match the position of the camera when collecting crop phenotypes, it is used as a background for crop phenotype shooting to block the complex environment behind the crop and reduce the cost of phenotype extraction. The required image processing work. In a more preferred implementation manner, the image acquisition devices on the two first phenotype acquisition units described above can be driven by servo motors and ball screw nut pairs of the same model. Set two first phenotype acquisition units in the initial state of the two image acquisition devices are located at the same starting position, two servo motors start at the same time, the two servo motors always maintain the same rotation and speed, so as to ensure the image acquisition equipment And the corresponding background board is driven synchronously. It is ensured that in the process of crop phenotype collection, the image collection equipment can correspond to the background board, and the background board is used as the background of the crop, which simplifies the steps of extracting the phenotypic characteristics of the crop from the complex environment.

The climate cabin control and analysis system in the climate cabin can control the XYZ three-direction servo motors to drive the XYZ three-directional sliding guides in real time according to the requirements of obtaining side view phenotype data of crop stems, leaves and roots, so as to drive the corresponding The phenotype acquisition sensor group realizes the acquisition of side view phenotype data of multiple groups of crops. The climate cabin control and analysis system can control the phenotype acquisition sensor group to acquire multiple sets of crop side view phenotype data in real time, timed, and at fixed points, and process the data such as storage and image recognition feature extraction, and then complete multiple sets of crop side views Phenotypic data storage, transmission and phenotypic data analysis.

In some implementations, the monitoring system can be further installed on the top of the climate chamber. As a result, the climate cabin control and analysis system can monitor the conditions in the cabin in real time according to the needs of use, and can display it on the touch screen outside the cabin in real time.

In order to further monitor and adjust the environment where the crops are located during the entire process of crop cultivation and phenotype extraction, the present invention further sets up a mesh plate inside the container, and installs a gas system between the mesh plate and the inner wall of the container. It includes: air-conditioning device, CO ₂ generating or supplying device, O ₃ generating or supplying device, humidifying device, air mixing valve, ground exhaust pipe, side exhaust pipe, top air return pipe, ground mesh plate, side mesh board. The air-conditioning device has controllable environmental functions such as heating, cooling, fresh air, dehumidification; CO ₂ generation or supply device adopts gas storage tank, regular replacement of CO ₂ _{can provide CO 2} gas required for crop growth; O ₃ generation or supply device can provide _{O 3} gas required for crop disinfection and prevention of plant diseases and insect pests; humidification device can provide environmental humidification function; there are several air outlets on the ground exhaust pipe, which are installed at the bottom of the climate chamber box in the crop cultivation area II and the phenotype acquisition area III ; There are several air outlets on the side exhaust pipes, bottom air outlets and side air exhaust pipes, which supply air from bottom to top to adjust the indoor temperature, and return air from the top air return pipe to achieve gas circulation.

The above-mentioned gas systems are installed on both sides of the climate chamber box in the crop cultivation area II and the phenotype acquisition area III; there are several return air holes on the top air return pipe, which are installed in the crop cultivation area II and the phenotype acquisition area III. The top of the cabin box; the ground mesh plate is installed at the bottom of the climate cabin box in the crop cultivation area II and the phenotype acquisition area III, and can cover the ground exhaust pipe to achieve uniform exhaust at the bottom; the side mesh plate is installed On both sides of the climate chamber box in the crop cultivation area II and the phenotype acquisition area III, and can cover the side exhaust pipes on both sides to achieve uniform exhaust air on both sides; CO _{2 is arranged in the climate chamber close to the crop} The concentration detection device, the O ₃ concentration detection device and the humidity detection device obtain environmental parameters through detection and output to the control and analysis system control, which regulates the air-conditioning device, CO ₂ generation or supply device, and humidification device in the environmental equipment area IV to generate gas, The gas is matched by controlling the opening and closing degree of the valve core of the gas mixing valve. After the gas mixing valve adjusts the gas in the pipeline, the ground exhaust pipe and the side exhaust pipe will input the mixed gas into the crop cultivation area II and the phenotype acquisition area III of the climate chamber, so as to achieve uniform exhaust on the bottom and sides of the climate chamber. Wind and environmental factors are controlled. Then, the gas in the climate cabin is transported back to the air conditioning device through the top air return pipe, so as to realize the recycling of the gas in the entire climate cabin and reduce the overall energy consumption of the climate cabin.

In the above-mentioned crop cultivation area II and phenotype acquisition area III, the lighting system can also be installed on the top of the climate cabin, and the lighting system can be controlled on and off according to the needs of the use through the climate cabin control and analysis system.

The above-mentioned environmental parameters such as gas, temperature, humidity, and light can be obtained in real time through the monitoring system installed on the top of the climate cabin or near the crops. The climate cabin control and analysis system monitors the conditions in the cabin in real time according to the needs of use, and in real time outside the cabin. The above environmental parameters are displayed on the touch screen.

Therefore, the present invention can facilitate the cultivation of crops through the overall design of the crop root box, the phenotype acquisition unit and the climate chamber, and can also perform high-throughput, high-precision, and low-cost crop phenotype acquisition on crops at the same time. Analysis function. It uses the environmental control in the environmental cabin to simultaneously carry out high-throughput, high-precision, and low-cost crop phenotype acquisition and acquisition of crop stems, leaves and other aboveground organs and roots and other underground organs under the influence of different environmental factors. analysis.

The invention can simultaneously provide the functions of developing crop cultivation and high-throughput, high-precision, and low-cost crop phenotype acquisition and analysis; and can simultaneously provide the functions of developing crop stems, leaves and other aboveground organs and roots and other underground organs under the influence of environmental factors. High-throughput, high-precision, low-cost crop phenotype acquisition and analysis functions.

The above are only the embodiments of the present invention, and the description is relatively specific and detailed, but it should not be understood as a limitation to the patent scope of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and these all fall within the protection scope of the present invention.

Claims

A high-throughput acquisition device for crop phenotypes, which is characterized in that it is arranged in a climate chamber and includes:

The root boxes are arranged in a straight line along the first direction on the working plane, each of the root boxes is respectively set in a flat rectangular parallelepiped structure with transparent two sides, and the distance between the two transparent sides in the root box In the range of 10mm-20mm, the two transparent sides of each of the root boxes are arranged parallel to the first direction;

The first phenotype acquisition unit includes two that are arranged on the plane where the root box is located, and the two first phenotype acquisition units are respectively arranged on both sides of the root box parallel to the first direction, and each of the first phenotype acquisition units is arranged on both sides of the root box. The type obtaining unit is respectively used to obtain the phenotype information of the crops contained in the root box in a side view corresponding to the two transparent sides in the root box;

The second phenotype acquisition unit is arranged above the plane where the root box is located, and is used to acquire the phenotype information of the crop contained in the root box in a top view.
The device for obtaining a high-throughput crop phenotype according to claim 1, wherein the root box comprises:

A root box skeleton (13), which has a plurality of uprights, and a bottom plate connected to the bottom end of each of the uprights. The uprights and the bottom plate form a root accommodating space in a flat rectangular parallelepiped structure to accommodate the roots of crops. The edges of the bottom plate are also Set with a card slot;

Side baffles (42), which are arranged on the left and right sides of the root system accommodating space, and are fixedly connected to the uprights, and the bottom of the side baffles (42) is tightly connected to the edge of the bottom plate;

A light-transmitting plate (14), which is arranged on the front and back sides of the root accommodating space, and is plug-connected to the upright column, the side baffle (42) is made of transparent material, and the side baffle (42) The bottom of the root box is clamped into the card slot on the edge of the bottom plate, the light-transmitting plate (14), the column and the side baffle seal the root accommodating space; in each of the root boxes, the front and rear sides are transparent The distance between the light plates (14) is in the range of 10mm-20mm, and the light-transmitting plates (14) of each of the root boxes are arranged in a straight line along the first direction on the working plane;

A light-shielding plate, which is close to the light-transmitting plate (14), is arranged on the outer side of each of the light-transmitting plates (14), and is detachably connected to each of the uprights in the root accommodating space;

The upper end cover (12) of the root box is fixedly connected to the upper end of each of the uprights, and the middle part of the upper end cover (12) of the root box is provided with a through hole for accommodating the growth of crops.
5. The crop phenotype high-throughput acquisition device according to claim 1-2, wherein each of the first phenotype acquisition units comprises:

The sliding guide rail (1) in the first direction is parallel to the transparent side surface of the root box of the crop, and is arranged on the outside of the root box along the first direction;

A sliding plate (31), which is arranged on the sliding guide rail (1) in the first direction, and the sliding guide rail (1) is translated in the first direction along the first direction;

The second direction sliding guide rail (32), the lower end of which is fixedly connected with the sliding plate (31), the second direction sliding guide rail (32) is kept perpendicular to the upper surface of the sliding plate (31), and is arranged along the second direction;

The third-direction sliding guide rail (33) is connected to the second-direction sliding guide rail (32), and the root box facing the crop is set in the third direction;

An image acquisition device (3), which is arranged at one end of the third-direction sliding guide (33) facing the root box, and is used to collect the root box and/or the crops contained in the root box when the root box corresponds to the root box. The image from the side view of the transparent two sides in the box;

A background board (4), which is arranged on one side of the image acquisition device (3);

When the third-direction sliding guide rail (33) moves in the second direction along the second-direction sliding guide rail (32), the image acquisition device (3) is driven to move synchronously, and the image acquisition device (3) is adjusted Relative to the height of the root box and/or the crops contained in the root box; when the third-direction sliding guide (33) moves in the third direction relative to the second-direction sliding guide (32), the driving The image acquisition device (3) moves synchronously to adjust the distance of the image acquisition device (3) relative to the root box and/or the crop contained in the root box.
3. The device for obtaining a high-throughput crop phenotype according to claims 1-3, wherein the second phenotype obtaining unit comprises:

The top sliding guide rail (53) includes two sliding guide rails (1) parallel to the first direction and fixed above the root box of the crop in the first direction, and the two top sliding guide rails (53) are respectively Set on both sides of the root box of the crop;

A middle sliding guide rail (52), both ends of which are respectively connected to two top sliding guide rails (53), and the middle sliding guide rail (52) translates along the first direction on the lower side of the top sliding guide rail (53) ；

The lower sliding guide rail (51) is connected to the middle sliding guide rail (52) at its upper end, a top view image acquisition device (5) is fixed at its lower end, and the lower sliding guide rail (51) is perpendicular to the middle sliding guide rail (52). ) And the top sliding guide rail (53), which moves relative to the middle sliding guide rail (52) in the second direction;

The top view image capture device (5) is fixed at the lower end of the lower sliding guide rail (51) downwardly toward the top of the root box, and the top view image capture device (5) is used to capture the root The image of the crop contained in the box and/or root box in a top view.
A climate cabin, characterized in that it comprises:

In the crop cultivation area (II), there are ground exhaust ducts (21), side exhaust ducts (22), top air return ducts (23), the ground exhaust ducts (21) and/or side The exhaust pipe (22) is connected to the output end of the air mixing valve. The input end of the air mixing valve is connected with an air conditioning device, a carbon dioxide supply device, an ozone supply device, and a humidification device. The gas in the crop cultivation area (II) is sent back to the air-conditioning device and then circulated and supplied to the crop cultivation area (II) by the air-conditioning device through the ground exhaust pipe (21) and/or the side exhaust pipe (22) );

The phenotype acquisition area (III) is provided with an electric sliding door, one side of the electric sliding door is connected to the phenotype acquisition area (III), and the other side of the electric sliding door is connected to the crop cultivation area (II) ), the phenotype acquisition area (III) is provided with the crop phenotype high-throughput acquisition device according to claim 1;

Environmental equipment area IV is used for fixed installation of the air-conditioning device, carbon dioxide supply device, ozone supply device, and humidification device.
The climate chamber according to claim 5, characterized in that the ground exhaust pipe (21), the side exhaust pipe (22), and the top return air pipe (23) extend from the crop cultivation area (II). Connected to the phenotype acquisition area (III), the crop cultivation area (II) and the phenotype acquisition area (III) are also provided with an environmental sensor group, and the environmental sensor group is used to collect the crop cultivation The environmental data in the area (II) and the phenotype acquisition area (III) adjust the operating status of the air conditioning device, the carbon dioxide supply device, the ozone supply device, and the humidification device.
The climate chamber according to claim 6, wherein the crop cultivation area (II) is further provided with a crop cultivation rack (8), the crop cultivation rack (8) is arranged in a multi-layer structure, and the crop A plurality of root box racks (7) parallel to each other are arranged in each layer of the cultivating rack (8); the top layer of the crop cultivating rack (8) and the partitions (6) between each layer structure are respectively arranged ), a spray nozzle (62) is arranged in the middle of the partition (6) for spraying moisture and nutrients to the crops below, and a plurality of supplementary lights are evenly arranged in the length direction of the partition (6) (61) is used to provide light, the upper surface of the partition (6) is set to be convex on all sides, and the middle is concave and flat, and the upper surface of the partition (6) is used to collect the water and nutrients sprayed on the upper layer ；

The root box rack (7) is a long plate structure, and a plurality of root box installation grooves are provided along the length direction of the long plate structure, and each root box passes through each of the root box installation grooves, and is covered by the upper end of the root box. The lower edge of (12) abuts the upper surface of the root box installation groove, and each of the root boxes is fixed under the long plate structure. Both ends of the root box frame (7) are also respectively provided with handles ( 71), a groove structure (72) is provided under the handle (71), and the groove structure (72) is clamped and fixed to the crop cultivation rack (8), and each of the root box racks (7) ) Is erected between the partitions (6) of each layer of the crop cultivation rack (8).
The climate cabin according to claims 6-7, wherein the environmental sensor group includes a light sensor, a humidity sensor, a temperature sensor, and a CO 2 sensor, and the environmental sensor group is installed on each shelf of the crop cultivation rack. The side of the plant is set close to the growing area of the crop in the root box.
The climate chamber according to claim 5, wherein the crop cultivation area (II), the phenotype acquisition area (III) and the environmental equipment area (IV) are arranged in the same container, and are enclosed by the container.