CN107047268B - The cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation - Google Patents
The cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation Download PDFInfo
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- CN107047268B CN107047268B CN201710251793.8A CN201710251793A CN107047268B CN 107047268 B CN107047268 B CN 107047268B CN 201710251793 A CN201710251793 A CN 201710251793A CN 107047268 B CN107047268 B CN 107047268B
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- 241000196324 Embryophyta Species 0.000 claims abstract description 22
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
- H04N7/181—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast for receiving images from a plurality of remote sources
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Signal Processing (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The present invention relates to root system high throughput cultivation under a kind of full dark situation and automation growth imaging systems, the system includes: main frame, for cultivating the root box of plant root, it is mounted on the root box frame that can be moved on main frame and relative to main frame, it is mounted on the H-type rack that can be moved on main frame and relative to main frame, it is mounted in H-type rack and can be relative to the imaging subsystems of H-type gantry motion, the irrigation subsystem for being mounted on main frame and being connected with root box, the return water subsystem for being mounted on main frame and being connected with irrigation subsystem, with for covering the shading subsystem for realizing full dark situation inside whole system；Wherein, multiple boxes are removably mounted on root box frame；Imaging subsystems are for being imaged the root system in root box；It irrigates subsystem and is used to provide nutrient solution realization soilless cultivation for the plant in root box；Return water subsystem is recycled for recycling the extra nutrient solution oozed out from root box.
Description
Technical field
The present invention relates to roots under the root system phenotype test platform more particularly to a kind of full dark situation under a kind of non-soil media
The high-throughput cultivation of system and automation growth imaging system.
Background technique
Plant phenotype is the key that understand plant genotype and environment interaction.In recent years, with the effect of gene sequencing technology
Rate is promoted and cost reduces, and the genomic data of plant constantly increases and perfect；However, the acquisition of plant phenotype information is still with people
Based on work, heavy workload, data redundancy is poor, and need to measure to plant-destructive.Therefore, the systematicness of plant phenotype information is received
Collection is increasingly becoming a limiting factor of genetic breeding research and base plants scientific research, and many academic institutions and enterprise endeavour
In the research and development of plant phenotype measurement platform.Currently, the research and development of plant phenotype determination techniques are mostly focused on plant shoot, this master
If causing the observation of root system and measurement all very tired due to the opacity of root growth medium and the complexity of root structure
It is difficult.Root system be plant absorption moisture, nutrients resource and respond soil environmental background value vitals, by improvement root traits come
Crop yield and nutrients resource utilization efficiency can be effectively improved by cultivating new varieties.Therefore, carry out the root system phenotype of indoor growing without
Damage measurement, deeply excavates crop gene-environment-phenotype interaction mechanism, to crop breeding research, plant physiology research and model
Parametrization has vital effect.
Currently, the root system non-destructive determination method of indoor growing includes NMR imaging method, neutron chromatography and micro- X-ray
Method etc..Micro- x-ray method is most accurate nondestructively measuring method, but can only measure the seedling stage root in the earth pillar of diameter very little
System, equipment cost is high, a large amount of data space and powerful computer set group operatione is needed, in high-throughput root system phenotype
The application of measurement aspect is restricted.Root box photographic process (GROWSCREEN-Rhizo, 2012) can cultivating plants, essence with high throughput
Standard irrigates, automatically the root system in narrow box is imaged, but can not obtain complete root structure, and equipment cost compared with
Height, space utilization rate are low.Obtaining the root system phenotype under non-soil media is to carry out another important channel of root system research.It is based on
The Rhizotubes (2015) of LemnaTec system can be tightly attached to the root system image on round tube surface by large batch of acquisition, but
It is the raising with flux, the distance of plant transmission also increases, and the transport of long range and frequent start and stop influence plant
It grows (lodging).The scientific research personnel such as Adu (2014), as being imaged and cultivating main body, germination paper are affixed on and is swept using sweep optical square
The transparent panel of instrument is retouched, the therebetween growth of root system, the bottom of germination paper, which is protruded into, draws nutrient in nutrient solution.This method will germinate
Paper is used as somatomedin, Nutrient Absorption medium and light screening material simultaneously.But with the raising of flux, the quantity of scanner also with
Equal proportion increase, and be limited by the water absorbing capacity of germination paper, this method can not cultivate biggish plant.To sum up, existing
Be phenotype test platform can not high throughput, full-automation, the real time measure, accurately growing environment control and low cost between
Obtain good balance.Therefore, urgent need designs and develops a set of high-throughput, automation now, real-time root system phenotype quickly measures
Platform deeply excavates crop gene-environment-phenotype interaction mechanism to meet the requirement of scale scientific research.
Summary of the invention
In view of the above-mentioned problems, the object of the present invention is to provide the cultivation of root system high throughput and automatic metaplasias under a kind of full dark situation
Long imaging system can carry multiple groups root box frame, and single root box frame can carry multiple boxes, by control imaging subsystems three
Maintenance and operation is dynamic and rotation and the sliding of root box frame, integrate irrigate, return water and shading subsystem, realize under full dark situation to root
It is the real time imagery of growth, and is taken between high-throughput, full-automatic, the real time measure and space-efficient and low cost
It obtains and balances well, can satisfy the requirement of scale scientific research, provided completely to analyze various root system phenotypic character parameters
Solution.
To achieve the above object, the present invention takes following technical scheme: under a kind of full dark situation the cultivation of root system high throughput and
Automation growth imaging system, which is characterized in that the system includes: main frame, for cultivating the root box of plant root, is mounted on
On the main frame and the root box frame that can be moved relative to the main frame, it is mounted on the main frame and can be relative to described
Main frame movement H-type rack, be mounted in the H-type rack and can relative to the imaging subsystems of the H-type gantry motion,
The irrigation subsystem for being mounted on the main frame and being connected with described box, be mounted on the main frame and with the filling
The return water subsystem that subsystem is connected is irrigate, and for covering the shading subsystem for realizing full dark situation inside whole system；
Wherein, multiple described boxes are removably mounted on described box frame；The imaging subsystems are used for in described box
Root system is imaged；The irrigation subsystem, which is used to provide nutrient solution for the plant in described box, realizes soilless cultivation；It is described
Return water subsystem is recycled for recycling the extra nutrient solution oozed out from described box.
The main frame includes more upper beams and underbeam, is connected between the upper beam and underbeam by vertical beam；It is parallel opposite
Two upper beams top and inside linear top guide rail and inside linear guide are fixedly installed respectively, parallel opposite two
The bottom linear guide and bottom ball wire parallel with the two inside linear guides are respectively set on the inside of underbeam described in root
Bar, sliding is provided with top slide, interior sideslip respectively in the linear top guide rail, inside linear guide and bottom linear guide
Block and base slider；Described box frame is mounted on the main frame by the top slide and can be relative to the main frame
Movement；The H-type rack is mounted on the main frame by the inside block and base slider and can be relative to the master
Frame movement.
Described box includes centrally disposed culture body, the background cloth for being covered on culture body front and back two sides, difference
Be arranged in it is described culture body left and right sides and bottom edge spacer bar, be arranged in the background cloth outside and with the spacer bar
The transparent splint being fixedly connected and the root box irrigation pipe being arranged at the top of the culture body；It is described culture body thickness with it is described
The consistency of thickness of spacer bar；The spacer bar of the spacer bar of the culture body bottom apart from the culture body and two sides
Bottom a distance forms the rhone in described cassette bottom portion；Described box irrigation pipe is connected with the irrigation subsystem.
Described box frame includes that the top beam of two parallel intervals setting and the top beam are set in parallel in two top beams
Between the bottom beam of underface being spaced and the vertical beam for being connected as one the top beam and bottom beam, the top beam, bottom beam and
The loading space of described box is surrounded between vertical beam；The length of the top beam is more than or equal to the width of the main frame, passes through
The both ends of the top beam are fixedly connected with the top slide of the main frame respectively, so that described box frame is mounted on
On the main frame；The length of the bottom beam is less than the width of the main frame.
The H-type rack includes the level that the vertical slide unit of two sides, both ends are slidably connected with the two vertical slide units respectively
Crossbeam slide unit, the simultaneously pedestal that is fixedly connected with the bottom of the two vertical slide units and it is slidably arranged in the horizontal gird slide unit
On turntable, the two vertical slide units and the horizontal gird slide unit constitute H-type, and the imaging subsystems are fixedly mounted on institute
It states on turntable；The both ends of the pedestal are fixedly connected with two base sliders of the main frame respectively, and two is described vertical
The top side of slide unit is fixedly connected with two inside blocks of the main frame respectively, so that the H-type rack can be opposite
It is slided in the main frame；The horizontal gird slide unit can be relative to the H-type rack lifting carriage；The turntable can be opposite
It is slided in the H-type rack level；The turntable can be rotated around itself 360 ° of vertical rotary shaft.
The vertical slide unit includes vertical line guide rail and vertical ball screw, the vertical slipper of the vertical line guide rail
It is connected with the down-feed screw nut of the vertical ball screw, the motor vertical driving of the vertical ball screw is described vertical
Feed screw nut's movement, so that the vertical slipper be driven to slide relative to the vertical line guide rail；The horizontal gird slide unit
Both ends be fixedly connected respectively with the vertical slipper on the vertical line guide rail of the two vertical slide units, it is described vertical
Ball screw drives the vertical slipper movement to drive the horizontal gird slide unit lifting；The horizontal gird slide unit includes level
Linear guide and horizontal ball screw, the horizontal lead screw of the horizontal slider of the horizontal linear guide rail and the horizontal ball screw
Nut is connected, and the horizontal motor of the horizontal ball screw drives the horizontal lead screw nut movement, to drive the water
Smooth block is slided relative to the horizontal linear guide rail；The bottom feed screw nut of the bottom ball screw and the bottom of the pedestal
Seat crossbeam is fixedly connected, and the bottom motors of the bottom ball screw drive the bottom feed screw nut movement, to drive institute
State H-type housing slide；The turntable is fixedly connected with the horizontal slider on the horizontal linear guide rail of the horizontal gird slide unit,
The horizontal ball screw drives the horizontal slider movement that the turntable is driven to slide relative to the horizontal gird slide unit.
The imaging subsystems include that the imaging device being fixed on the turntable, lamp cap are fixedly mounted facing downward
Flash lamp below the horizontal gird slide unit and the reflector being fixed at below the flash lamp lamp cap.
The irrigation subsystem includes by the sequentially connected nutrient solution tank of connecting pipe, irrigates self priming pump and circulation self-priming
Pump；The input end of each described box irrigation pipe passes through lateral and the connecting pipe for irrigating self priming pump one end respectively
It is connected, the outlet end of each described box irrigation pipe passes through the connection of each lateral and described nutrient solution tank one end respectively
Pipeline is connected；Non-return valve and the first solenoid valve, institute are additionally provided in the connecting pipe of described box irrigation pipe input end
It states and is additionally provided with second solenoid valve in the connecting pipe of root box irrigation pipe outlet end；
The return water subsystem include water tank, both ends closure and at one end the sink of bottom opening, one end be closed and it is another
The diving for holding the U-type groove being connected with the water tank and being arranged in the water tank and being connected with the irrigation subsystem
Pump；Multiple sinks are respectively fixedly disposed at the bottom of each described box frame, and the aperture of the bottom of gullet is towards together
Side；The aperture of the bottom of gullet connects aqueduct, and the aqueduct bottom end is connected with filter cloth bag；The U-type groove is solid
The main frame bottom side is set calmly and is located at the underface of the filter cloth bag.
The shading subsystem includes antiglare module and main frame side between root box frame top beam interval antiglare module, root box frame
Antiglare module；Wherein, between described box frame top beam interval antiglare module is used between two top beams of described box top of the trellis
Every filling；Antiglare module is between described box frame and between described box frame and the main frame between described box frame
Shading；Main frame side antiglare module is used for the shading of the main frame surrounding.
Antiglare module includes the micro- convergent force organ cover of N-shaped and suspension hanging beam, the micro- convergent force wind of N-shaped between described box frame
The both ends in the scalable direction of qin cover respectively with the top beam of the top beam of two described box framves or one described box frame and
The upper beam of one main frame is fixedly connected, and the sliding of described box frame can drive the micro- convergent force organ cover of the N-shaped
It is flexible；The more suspension hanging beams are fixed at the master with being parallel to the micro- scalable direction of convergent force organ cover of the N-shaped
Frame roof is suspended in the suspension hanging beam in the middle part of the micro- convergent force organ cover of N-shaped；Main frame side photomask
Block is sewed with the side black-out cloth of waterproof slide fastener using three Bian Shangjun, by by the side shading of the main frame surrounding
Cloth is connected after being sutured by the waterproof slide fastener, realizes the shading of the main frame surrounding.
The invention adopts the above technical scheme, which has the following advantages: 1, root under a kind of full dark situation of the invention
The high-throughput cultivation of system and automation growth imaging system, by integrated root box, irrigate subsystem, return water subsystem, shading subsystem
System and real time imagery subsystem have cultivation, measurement and monitoring function concurrently, realize in no manual intervention to root system completely black
Real time imagery and monitoring in dark situation, the real-time reflection convenient for observation root system to various experiment process.2, one kind of the invention
The cultivation of root system high throughput and automation growth imaging system, root box can be used in observing complete root structure under full dark situation,
Root system during the growth process fix by its position, is loaded by the cooperation with root box frame, what the transparent splint of root box applied root system
Pressure is uniform, improves the repeatability of experimental result.3, the cultivation of root system high throughput and automation under a kind of full dark situation of the invention
Imaging system is grown, integrates all motor functions by using H-type rack, the imaging device of imaging subsystems is enable to exist
Tri- directions XYZ move in a straight line and rotary motion, and root box frame, which can also synchronize, to slide back and forth, to realize on multiple box framves
The imaging of root system realizes high throughput automated the real time measure.4, root system high throughput is planted under a kind of full dark situation of the invention
Training and automation growth imaging system, shading subsystem not only solves the demand that root system is grown in full dark situation, in imaging
Also not to imaging sight cause to block, each antiglare module is easy to disassemble, facilitate installation root box and transplant seedlings and other operation.
5, the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation of the invention, modules can independent operating
Without interfering with each other, whole system is compact-sized, occupies little space, and scalability and scalability are strong.6, the present invention is a kind of complete dark
The cultivation of root system high throughput and automation growth imaging system, can satisfy the requirement of scale scientific research, for analysis under environment
Various root system phenotypic character parameters provide total solution, in crop cultivation, botany, environmental science, genotype and ring
There is very high application value in the fields such as border interaction.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of main frame of the invention；
Fig. 3 is the dimensional decomposition structure diagram of root box of the invention；
Fig. 4 is the left view structural representation of root box of the invention；
Fig. 5 is the structural schematic diagram of root box frame of the invention；
Fig. 6 is the structural schematic diagram of H-type rack of the invention；
Fig. 7 is root box frame of the invention, H-type rack, the assembling schematic diagram of imaging subsystems and main frame；
Fig. 8 is the structural schematic diagram of irrigation subsystem and return water subsystem of the invention；
Fig. 9 is the assembly signal for irrigating subsystem, return water subsystem, root box frame, H-type rack and main frame of the invention
Figure；
Figure 10 is the structural schematic diagram of shading subsystem of the invention；
Figure 11 is the partial structural diagram of shading subsystem of the invention.
Specific embodiment
The present invention is described in detail below with reference to the accompanying drawings and embodiments.
As shown in Figure 1, the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation provided by the invention
System comprising main frame 1, root box frame 3, H-type rack 4, imaging subsystems 5, irrigates subsystem 6,7 and of return water subsystem at root box 2
Shading subsystem 8.
As shown in Fig. 2, main frame 1 is the cube frame of 2.5m long, the 2.5m wide and 1.5m high that are assembled by aluminum profile,
Including four upper beams, 11, four underbeams 12 and four vertical beams 13；Wherein, the top and inside of two opposite upper beams 11 are solid respectively
Linear top guide rail 14 and inside linear guide 15 are set surely；Meanwhile the inside of two opposite underbeams 12 be respectively set with
The parallel bottom linear guide 16 of two inside linear guides 15 and bottom ball screw 17, linear top guide rail 14, inside are straight
Sliding is provided with top slide 141, inside block 151 and base slider 161 respectively on line guide rail 15 and bottom linear guide 16.
In above-described embodiment, be provided with multiple top slides 141 on linear top guide rail 14, root box frame 3 also include it is multiple,
To realize high-throughput cultivation.
As shown in Figure 3, Figure 4, root box 2 includes centrally disposed plate culture body 21, is covered on 21 front and back two of culture body
The background cloth 22 in face, the hard spacer bar 23 for being separately positioned on culture 21 left and right sides of body and bottom, setting are in culture body 21
The outside of the background cloth 22 on front and back two sides and the transparent splint 24 being fixedly connected with spacer bar 23 and setting are pushed up in culture body 21
The root box irrigation pipe 25 in portion；Wherein, the thickness of body 21 and the consistency of thickness of spacer bar 23 are cultivated, so that transparent splint 24 can will be trained
It supports body 21 and background cloth 22 is clamped in centre, the spacer bar 23 of hard can play the role of support to transparent splint 24, reduce saturating
The deformation of bright clamping plate 24 is heaved；The spacer bar 23 of body 21 bottom is cultivated apart from culture body 21 and left and right sides spacer bar 23
Bottom a distance is conducive to the nutrient solution discharge root box 2 of lower infiltration to form the rhone 26 of 2 bottom of root box；Root box is irrigated
It is provided with two rows of micropores on pipe 25, micro-pore diameter 0.3mm, 150 ° of angle between two rows of micropores.Culture body 21 can be sponge or
Person's aperture EPDM (Ethylene Propylene Diene Monomer, ethylene propylene diene rubber) material (ultra-soft).
In above-described embodiment, transparent splint 24 is made of clear polycarbonate, thickness 6mm；Cultivate the thickness of body 21
20mm；Spacer bar 23 is made of acrylic board, thickness 20mm, width 20mm, the length left and right sides with culture body 21 respectively
The length on side and bottom edge is adapted.
In above-described embodiment, corresponding position is drilled with circular hole, transparent folder in the surrounding and spacer bar 23 of transparent splint 24
It is connected between plate 24 and spacer bar 23 by bolt and nut.
As shown in Figure 5, Figure 7, root box frame 3 includes top beam 31, bottom beam 32 and vertical beam 33, and two top beams, 31 parallel interval is set
It sets, the length of top beam 31 is more than or equal to the width of main frame 1, by leading the both ends of top beam 31 and the linear top of main frame 31
The top slide 141 of rail 14 is fixedly connected, so that root box frame 3 is mounted on main frame 1, and realizes the sliding of root box frame 3；Bottom
Beam 32 and two top beams 31 are set in parallel in the underface of two top beams, 31 midfeather, and the length of bottom beam 32 is less than main frame 1
Width facilitates root box frame 3 to move in main frame 1；The loading of root box 2 is surrounded between top beam 31, bottom beam 32 and vertical beam 33
Space.
In above-described embodiment, the top beam 31 of root box frame 3 is made of the aluminum profile of 20 × 20mm, length 2.5m；Bottom beam 32 by
The aluminum profile of 40 × 40mm is made, length 2.1m.
In above-described embodiment, in order to ensure pressure of the transparent splint 24 to any position root system is consistent, the top beam of root box frame 3
Root box fixed link (not shown) is additionally provided on 31, root box fixed link is made of the aluminum corner brace of 20 × 10mm, length and top
The length of beam 31 is identical, and root box fixed link is fixedly connected by broadside with top beam 31, passes through the transparent splint 24 of narrow side and root box 2
It is fixedly connected；Two box fixed links being arranged on two sides top beam 31 apply pressure to the transparent splint 24 at 2 top of root box, it is ensured that
The interval of two transparent splints 24 of root box 2 is in any position (especially 2 top of root box) 20mm.In use, first by one
Box fixed link is fixedly connected with a top beam 31, by root box 2 by the loading space of the interval insertion root box frame 3 among two top beam 31
In, root box 2 is placed on bottom beam 32, and the transparent splint of side 24 is fixedly connected with root box fixed link；It then will be another
Root box fixed link is fixedly connected with another top beam 31 again after clamping 2 top of root box.
As shown in Figure 6, Figure 7,4 width 2.3m, high 1.4m of H-type rack comprising the vertical slide units 41 of two sides, both ends respectively with
Horizontal gird slide unit 42 that two vertical slide units 41 are connected, the pedestal 43 that the bottom of slide unit 41 vertical with two is fixedly connected simultaneously and
Turntable 44 on horizontal gird slide unit 42 is set, and two vertical slide units 41 constitute H-type with horizontal gird slide unit 42.Wherein, two
The top side of vertical slide unit 41 is fixedly connected with the inside block 151 of two inside linear guides 15 of main frame 1 respectively, is used for
Ensure vertical slide unit 41 operation and it is static when do not glance off；Vertical slide unit 41 includes vertical line guide rail 411 and vertical rolling
Ballscrew 412, the vertical slipper 413 of vertical line guide rail 411 are connected with the down-feed screw nut 414 of vertical ball screw 412
It connects, the motor vertical 415 of vertical ball screw 412 drives down-feed screw nut 414 to move, to drive 415 phase of vertical slipper
Vertical line guide rail 411 is slided；The vertical line guide rail of the both ends of horizontal gird slide unit 42 slide unit 41 vertical with two respectively
Vertical slipper 413 on 411 is fixedly connected, and vertical ball screw 412 drives vertical slipper 413 by down-feed screw nut 414
Movement drives horizontal gird slide unit 42 to go up and down, and horizontal gird slide unit 42 includes horizontal linear guide rail 421 and horizontal ball screw
422, the horizontal slider 423 of horizontal linear guide rail 421 is connected with the horizontal lead screw nut 424 of horizontal ball screw 422, horizontal
The horizontal motor 425 of ball screw 422 drives horizontal lead screw nut 424 to move, to drive horizontal slider 423 relative to level
Linear guide 421 is slided；The both ends of pedestal 43 are fixed with the base slider 161 of two bottom linear guides 16 of main frame 1 respectively
Connection, the bottom feed screw nut 171 of bottom ball screw 17 are fixedly connected with the base crossbeam of pedestal 43, bottom ball screw 17
Bottom motors 172 drive bottom feed screw nut 171 move, so that H-type rack 4 be driven to slide back and forth；Turntable 44 and level
Horizontal slider 423 on the horizontal linear guide rail 421 of crossbeam slide unit 42 is fixedly connected, and horizontal ball screw 422 drives horizontal cunning
The movement of block 423 drives turntable 44 to horizontally slip relative to horizontal gird slide unit 42, and turntable 44 can be around the vertical of itself
360 ° of rotary shaft rotations.The top of vertical slide unit 41 is further fixedly arranged on stepper motor 416,417 and of matched planetary reduction gear
Rotating arm 418, rotating arm 418 and the axis of planetary reduction gear 417 are fixed；When H-type rack 4 slides into closely root box frame 3 and rotates
When arm 418 moves to state vertically upward, H-type rack 4 and root box frame 3 can be locked together, H-type rack 4 slides back and forth can
Push-and-pull root box frame 3 moves synchronously；And when rotating arm 418 keeps state vertically downward, H-type rack 4 can be below root box frame 3 certainly
By walking and root box frame 3 remain stationary state.
As shown in fig. 7, imaging subsystems 5 include camera 51, flash lamp 52 and reflector 53, wherein camera 51 is fixed on
On turntable 44,51 lamp cap of flash lamp is fixed on the lower section on horizontal slider 423 and being located at horizontal gird slide unit 42 facing downward, dodges
A reflector 53 is fixedly installed in the position of 10cm below 51 lamp cap of light lamp.
As shown in Figure 8, Figure 9, subsystem 6 is irrigated to include nutrient solution tank 61, irrigate self priming pump 62, circulation self priming pump 63, connect
Adapter tube road 64, non-return valve 65, the first solenoid valve 66 and second solenoid valve 67.Wherein, the input end difference of each box irrigation pipe 25
It is connected by each lateral with connecting pipe 64, then passes through connecting pipe 64 and irrigation self priming pump 62, circulation self priming pump
63 are connected with nutrient solution tank 61, and non-return valve 65 and the first solenoid valve 66 are provided in the connecting pipe 64 of input end；Each
The outlet end of box irrigation pipe 25 is connected by each lateral with connecting pipe 64 respectively, then passes through connecting pipe 64 and battalion
Nutrient solution bucket 61 is connected, and second solenoid valve 67 is provided in the connecting pipe 64 of outlet end.When not needing to irrigate, the first electricity
Magnet valve 66 and second solenoid valve 67 are in close state；When irrigating, the first solenoid valve 66 is in opening state, irrigates self-priming
62 starting of pump, draws nutrient solution from nutrient solution tank 61, the root box irrigation pipe 25 of different boxes 2 is moved to by connecting pipe 64
In, nutrient solution is provided to plant；At the end of irrigation, second solenoid valve 67 is opened, closes and irrigates self priming pump 62, it is ensured that is irrigated vertical
Quarter terminates；When circulation, the first solenoid valve 66 and second solenoid valve 67 are opened simultaneously, and nutrient solution is existed by recycling self priming pump 63
It is recycled in connecting pipe 64 and root box irrigation pipe 25 is not discharged, connecting pipe after nutrition fluid exchange is ensured by the circulation of nutrient solution
Nutrient solution concentration is consistent in 64.
As shown in Figure 8, Figure 9, return water subsystem 7 includes sink 71, U-type groove 72, water tank 73 and immersible pump 74.Wherein, often
A sink 71 is fixedly installed in the bottom of a piece box frame 3, for access by the extra nutrition that is discharged in the rhone 26 of root box 2
Liquid, the both ends closure of sink 71, one end bottom opening, and waterproof connector is installed, waterproof connector connection aqueduct 711 (can be
A piece PE pipe) it is used for water guide, 711 bottom end of aqueduct is connected with filter cloth bag 712, for filtering nutrient solution.U-type groove 72 is fixed
The side of 1 bottom of main frame is set and is located at the lower section of the filter cloth bag 712 of sink 71, one end closure of U-type groove 72 is another
End is connected with water tank 73, for arranging the nutrient solution of recycling to water tank 73；Immersible pump 74 is arranged in water tank 73 and and nutrition
Liquid bucket 61 is connected, and the recycling nutrient solution in water tank 73 is pumped into nutrient solution tank 61 by 74 timing of immersible pump, realizes nutrient solution
It recycles.
In above-described embodiment, position fixed setting corresponding with U-type groove 72 on the horizontal gird slide unit 42 of H-type rack 4
One arch rhone 426, filter cloth bag 712 can be slipped over close to arch rhone 426, and 712 water dumping of filter cloth bag is avoided to get machine wet
Tool component.
In above-described embodiment, sink 71 and U-type groove 72 are made using PVC material, and 71 width 40mm of sink is long
2100mm, high 15mm.
As shown in Figure 10 and Figure 11, shading subsystem 8 includes shading between root box frame top beam interval antiglare module 81, root box frame
Module 82 and main frame side antiglare module 83.Wherein, root box frame top beam interval antiglare module 81 is for two at the top of root box frame 3
The filling being spaced between top beam 31 specifically includes three for hole filling between plant and 3 middle top of root box frame inside
Horizontal bore nitrile rubber 811, the link block 812 being individually fixed at the top of the vertical beam 33 at 3 both ends of root box frame, and for connecting
Connect the vertical open pores nitrile rubber 813 of 31 end gap seals of block 812 and two top beams.Antiglare module 82 is used for root box between root box frame
Shading between frame 3 and between root box frame 3 and main frame 1, including the micro- convergent force organ cover 821 of N-shaped and suspension hanging beam 822, N-shaped
The both ends of micro- convergent force organ cover 821 respectively with the top beam 31 of two box framves 3 or with the top beam 31 and main frame of a box frame 3
1 upper beam 11 is fixedly connected, and the sliding of root box frame 3 can drive the flexible of the micro- convergent force organ cover 821 of N-shaped；4 suspension hanging beams
822 both ends are separately fixed on two upper beams 11 at 14 both ends of linear top guide rail, and multiple hanging rings are provided in suspension hanging beam 822
8221 and suspension hook 8222, the micro- convergent force organ cover 821 of N-shaped is suspended in suspension hanging beam 822 by hanging ring 8221 and suspension hook 8222,
Prevent the micro- convergent force organ cover 821 of N-shaped from sinking；To realize between adjacent two box framves 3 and root box frame 3 and main frame 1 it
Between shading.Main frame side antiglare module 83 is used for the shading of 1 surrounding of main frame, main anti-using being sewed in 3 Bian Shangjun
The side black-out cloth 831 of water zipper can facilitate the disassembly and connection of side black-out cloth 831；The side of 1 surrounding of main frame is hidden
Light cloth 831 is connected after being sutured by waterproof slide fastener, realizes the shading of 1 surrounding of main frame.
In above-described embodiment, the micro- convergent force organ cover 821 of N-shaped includes the long organ sheets 8211 of horizontal "-" type, vertical one
Font organ sheets 8212, interfolded organ sheets 8213, the long end plate 8214 of "-" type and L-type connecting plate 8215；It will be horizontal
The long organ sheets 8211 of "-" type and vertical "-" type organ sheets 8212 and interfolded organ sheets 8213 suture connection shape
At N-shaped organ cover unit, multiple N-shaped organ cover units are interconnected to form N-shaped organ cover unit group, can improve organ cover
Required external force when reducing flexible while flexible amplitude；Long 8214 both ends of end plate of "-" type are connect with L-type connecting plate 8215
N-shaped end plate is formed, the both ends of N-shaped organ cover unit group telescopic direction are connected with a N-shaped end plate respectively, so that it is micro- to form N-shaped
Convergent force organ cover 821；The micro- convergent force organ cover 821 of N-shaped passes through the N-shaped end plate at telescopic direction both ends and the top beam of root box frame 3
31 are fixedly connected.
In above-described embodiment, the outside that two upper beams 11 of linear top guide rail 14 are provided at the top of main frame 1 is fixed respectively
Long water box 19 are provided with, long water box 19 are located at vertical 8212 bottom of "-" type organ sheets of the micro- convergent force organ cover 821 of N-shaped；
It adds water to long water box 19 higher than vertical 8212 bottom of "-" type organ sheets, the outside of long water box 19 and side black-out cloth 831
It is fixedly connected, realizes the connection of the side black-out cloth 831 of the top micro- convergent force organ cover 821 of N-shaped and two sides, it can be in root box
Frame 3 and the micro- convergent force organ cover 821 of N-shaped realize sealing and shading in the case where sliding, and solve side black-out cloth 831 and bending
The micro- convergent force organ cover 821 of N-shaped be directly connected to the problem of complicated difficulty.
In above-described embodiment, the movement folding control of imaging subsystems 5, irrigation subsystem 6 and return water subsystem 7 is all made of
If Arduino microcontroller controls, the root box 2 of different box framves 3 different irrigation volume and nutrient solution circulation may be implemented.
The cultivation of root system high throughput and automation grow imaging system when in use under a kind of full dark situation of the invention, first
H-type rack 4 slides into away from a certain distance from first root box frame 3, and horizontal gird slide unit 42 rises to 2 middle part of camera 5 and root box
Parallel height, camera 5 from left to right slide into first root box, 2 front, successively take pictures to each root box 2；It
Horizontal gird slide unit 42 drops to bottom afterwards, and H-type rack 4 slide rearwardly to the centre of first root box 3 two top beam 31 of frame, rotation
Pivoted arm 418 is gone to vertically upward in the drive backspin of stepper motor 416, later 4 forward slip of H-type rack, while driving first
A root box frame 3 moves forward certain distance, and rotating arm 418 rotates to be vertically downward later；H-type rack 4 slide rearwardly to first
It is a between second root box frame 4, at a certain distance from first root box frame 3 of distance, camera 5 rotate 180 °, horizontal gird slide unit
42 rise to the height parallel with 2 middle part of root box of camera 5, and camera 5 successively from left to right claps the back side of each box 2
According to；For 4 forward slip of H-type rack at a certain distance from away from second root box frame 3, camera 5 rotates 180 ° later, starts shooting second
Root box 2 on a root box frame 3；And so on, complete taking pictures for all boxes 2.
The various embodiments described above are merely to illustrate the present invention, wherein the structure of each component, setting position and its connection type etc.
It may be changed, all equivalents and improvement carried out based on the technical solution of the present invention should not arrange
In addition in protection scope of the present invention.
Claims (10)
1. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation, which is characterized in that the system includes:
Main frame,
For cultivating the root box of plant root,
It is mounted on the root box frame that can be moved on the main frame and relative to the main frame,
It is mounted on the H-type rack that can be moved on the main frame and relative to the main frame,
Be mounted in the H-type rack and can relative to the imaging subsystems of the H-type gantry motion,
The irrigation subsystem for being mounted on the main frame and being connected with described box,
The return water subsystem for being mounted on the main frame and being connected with the irrigation subsystem, and
For covering the shading subsystem for realizing full dark situation inside whole system；
Wherein, multiple described boxes are removably mounted on described box frame；The imaging subsystems are used for described box
In root system be imaged；The irrigation subsystem, which is used to provide nutrient solution for the plant in described box, realizes soilless cultivation；
The return water subsystem is recycled for recycling the extra nutrient solution oozed out from described box.
2. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as described in claim 1
Sign is that the main frame includes more upper beams and underbeam, is connected between the upper beam and underbeam by vertical beam；It is parallel opposite
Linear top guide rail and inside linear guide is fixedly installed in the top and inside of two upper beams respectively, and parallel opposite two
The bottom linear guide and bottom ball screw parallel with the two inside linear guides are respectively set on the inside of the underbeam,
Sliding is provided with top slide, inside block respectively in the linear top guide rail, inside linear guide and bottom linear guide
And base slider；Described box frame is mounted on the main frame by the top slide and can be transported relative to the main frame
It is dynamic；The H-type rack is mounted on the main frame by the inside block and base slider and can be relative to the main frame
Frame movement.
3. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation as claimed in claim 1 or 2,
It is characterized in that, described box includes centrally disposed culture body, the background cloth for being covered on culture body front and back two sides, difference
Be arranged in it is described culture body left and right sides and bottom edge spacer bar, be arranged in the background cloth outside and with the spacer bar
The transparent splint being fixedly connected and the root box irrigation pipe being arranged at the top of the culture body；It is described culture body thickness with it is described
The consistency of thickness of spacer bar；The spacer bar of the spacer bar of the culture body bottom apart from the culture body and two sides
Bottom a distance forms the rhone in described cassette bottom portion；Described box irrigation pipe is connected with the irrigation subsystem.
4. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 2
Sign is that described box frame includes that the top beam of two parallel intervals setting and the top beam are set in parallel in two top beams
Between the bottom beam of underface being spaced and the vertical beam for being connected as one the top beam and bottom beam, the top beam, bottom beam and
The loading space of described box is surrounded between vertical beam；The length of the top beam is more than or equal to the width of the main frame, passes through
The both ends of the top beam are fixedly connected with the top slide of the main frame respectively, so that described box frame is mounted on
On the main frame；The length of the bottom beam is less than the width of the main frame.
5. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation as claimed in claim 2 or 4,
It is characterized in that, the H-type rack includes the level that the vertical slide unit of two sides, both ends are slidably connected with the two vertical slide units respectively
Crossbeam slide unit, the simultaneously pedestal that is fixedly connected with the bottom of the two vertical slide units and it is slidably arranged in the horizontal gird slide unit
On turntable, the two vertical slide units and the horizontal gird slide unit constitute H-type, and the imaging subsystems are fixedly mounted on institute
It states on turntable；The both ends of the pedestal are fixedly connected with two base sliders of the main frame respectively, and two is described vertical
The top side of slide unit is fixedly connected with two inside blocks of the main frame respectively, so that the H-type rack can be opposite
It is slided in the main frame；The horizontal gird slide unit can be relative to the H-type rack lifting carriage；The turntable can be opposite
It is slided in the H-type rack level；The turntable can be rotated around itself 360 ° of vertical rotary shaft.
6. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 5
Sign is that the vertical slide unit includes vertical line guide rail and vertical ball screw, the vertical slipper of the vertical line guide rail
It is connected with the down-feed screw nut of the vertical ball screw, the motor vertical driving of the vertical ball screw is described vertical
Feed screw nut's movement, so that the vertical slipper be driven to slide relative to the vertical line guide rail；The horizontal gird slide unit
Both ends be fixedly connected respectively with the vertical slipper on the vertical line guide rail of the two vertical slide units, it is described vertical
Ball screw drives the vertical slipper movement to drive the horizontal gird slide unit lifting by the down-feed screw nut；It is described
Horizontal gird slide unit includes horizontal linear guide rail and horizontal ball screw, the horizontal slider and the water of the horizontal linear guide rail
The horizontal lead screw nut of flat ball screw is connected, and the horizontal motor of the horizontal ball screw drives the horizontal lead screw nut
Movement, so that the horizontal slider be driven to slide relative to the horizontal linear guide rail；The bottom silk of the bottom ball screw
Stem nut is fixedly connected with the base crossbeam of the pedestal, and the bottom motors of the bottom ball screw drive the bottom screw rod
Nut movement, to drive the H-type housing slide；On the horizontal linear guide rail of the turntable and the horizontal gird slide unit
Horizontal slider be fixedly connected, the horizontal ball screw drives the horizontal slider movement to drive the turntable relative to institute
State the sliding of horizontal gird slide unit.
7. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 5
Sign is, the imaging subsystems include that the imaging device being fixed on the turntable, lamp cap are fixedly mounted facing downward
Flash lamp below the horizontal gird slide unit and the reflector being fixed at below the flash lamp lamp cap.
8. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 3
Sign is that the irrigation subsystem includes by the sequentially connected nutrient solution tank of connecting pipe, irrigates self priming pump and circulation self-priming
Pump；The input end of each described box irrigation pipe passes through lateral and the connecting pipe for irrigating self priming pump one end respectively
It is connected, the outlet end of each described box irrigation pipe passes through the connection of each lateral and described nutrient solution tank one end respectively
Pipeline is connected；Non-return valve and the first solenoid valve, institute are additionally provided in the connecting pipe of described box irrigation pipe input end
It states and is additionally provided with second solenoid valve in the connecting pipe of root box irrigation pipe outlet end；
The return water subsystem include water tank, both ends closure and at one end the sink of bottom opening, one end closure and the other end and
The U-type groove and the immersible pump for being arranged in the water tank and being connected with the irrigation subsystem that the water tank is connected；It is more
A sink is respectively fixedly disposed at the bottom of each described box frame, and the aperture of the bottom of gullet is towards the same side；
The aperture of the bottom of gullet connects aqueduct, and the aqueduct bottom end is connected with filter cloth bag；The U-type groove fixed setting
In the main frame bottom side and positioned at the underface of the filter cloth bag.
9. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 4
Sign is that the shading subsystem includes antiglare module and main frame side between root box frame top beam interval antiglare module, root box frame
Antiglare module；Wherein, between described box frame top beam interval antiglare module is used between two top beams of described box top of the trellis
Every filling；Antiglare module is between described box frame and between described box frame and the main frame between described box frame
Shading；Main frame side antiglare module is used for the shading of the main frame surrounding.
10. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 9
Sign is that antiglare module includes the micro- convergent force organ cover of N-shaped and suspension hanging beam, the micro- convergent force wind of N-shaped between described box frame
The both ends in the scalable direction of qin cover respectively with the top beam of the top beam of two described box framves or one described box frame and
The upper beam of one main frame is fixedly connected, and the sliding of described box frame can drive the micro- convergent force organ cover of the N-shaped
It is flexible；The more suspension hanging beams are fixed at the master with being parallel to the micro- scalable direction of convergent force organ cover of the N-shaped
Frame roof is suspended in the suspension hanging beam in the middle part of the micro- convergent force organ cover of N-shaped；Main frame side photomask
Block is sewed with the side black-out cloth of waterproof slide fastener using three Bian Shangjun, by by the side shading of the main frame surrounding
Cloth is connected after being sutured by the waterproof slide fastener, realizes the shading of the main frame surrounding.
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| CN107581040A (en) * | 2017-09-04 | 2018-01-16 | 西北农林科技大学 | The multifarious high flux culture systems of one kind identification Root inheritance and application |
| CN108308012A (en) * | 2018-02-08 | 2018-07-24 | 浦江县元寿农业科技有限公司 | Automate soilless-culture equipment |
| CN111226775B (en) * | 2019-02-11 | 2021-07-30 | 甘肃省祁连山水源涵养林研究院 | Plant root system observation device |
| CN110089307A (en) * | 2019-05-14 | 2019-08-06 | 南京农业大学 | A kind of root system of plant automation imaging system based on double cylinder cultivating containers |
| CN110612843A (en) * | 2019-10-28 | 2019-12-27 | 南京农业大学 | Crop phenotype high-throughput acquisition device and climate chamber |
| CN110617768A (en) * | 2019-10-28 | 2019-12-27 | 南京农业大学 | Root box for acquiring crop phenotype |
| CN113587953B (en) * | 2021-07-28 | 2023-09-15 | 西安中科微星光电科技有限公司 | Portable optical camera bellows |
| CN114041385B (en) * | 2021-11-15 | 2022-07-15 | 宁波市农业科学研究院 | Research method for root growth and development of vegetable and fruit crops |
| CN114190267A (en) * | 2022-01-11 | 2022-03-18 | 南京农业大学 | System for regulating and controlling growth environment of hydroponic plants and acquiring phenotype images |
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| US7278236B2 (en) * | 2005-03-17 | 2007-10-09 | Phenotype Screening Corporation | Plant root characterization system |
| CN103141320B (en) * | 2013-03-27 | 2015-07-22 | 西北农林科技大学 | Automatic plant root system monitoring system |
| CN104569972B (en) * | 2015-01-20 | 2017-01-18 | 中国科学院成都生物研究所 | Plant root system three-dimensional configuration nondestructive testing method |
| WO2017015549A1 (en) * | 2015-07-22 | 2017-01-26 | UHV Technologies, Inc. | X-ray imaging and chemical analysis of plant roots |
| CN105160584A (en) * | 2015-08-03 | 2015-12-16 | 中国林业科学研究院林业新技术研究所 | Method and equipment system for in situ dynamic monitoring of wetland plant root system |
| CN205679254U (en) * | 2016-06-16 | 2016-11-09 | 中国科学院南京土壤研究所 | Double-cylinder type plant roots in situ observation system device |
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Effective date of registration: 20191210 Address after: Room 511, building 6, South Science and Technology Innovation Park, No. 5, Tongwei Road, Xuanwu District, Nanjing City, Jiangsu Province Patentee after: Nanjing Gentu Agricultural Technology Co.,Ltd. Address before: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: China Agricultural University |
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