CN115943773A - Monitoring device and monitoring method for seed germination test - Google Patents

Monitoring device and monitoring method for seed germination test Download PDF

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Publication number
CN115943773A
CN115943773A CN202211559908.7A CN202211559908A CN115943773A CN 115943773 A CN115943773 A CN 115943773A CN 202211559908 A CN202211559908 A CN 202211559908A CN 115943773 A CN115943773 A CN 115943773A
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China
Prior art keywords
germination
water supply
image information
seeds
dish
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韩京京
潘荣辉
胡晋
关亚静
金益君
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Hainan Institute of Zhejiang University
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Hainan Institute of Zhejiang University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The invention relates to the technical field of crop seed research, and discloses a monitoring device for a seed germination test, which comprises: the germination device unit comprises a box body, a germination dish and a water supply assembly, wherein the germination dish and the water supply assembly are arranged in the box body; the image acquisition unit is used for periodically acquiring the image information of the seeds in the germination vessel and sending the image information; and the control unit is electrically connected with the image acquisition unit and used for receiving and storing the image information, comparing the image information of the nth stage with the image information of the 1 st stage, and obtaining the germination information of the nth stage through comparison and calculation, wherein n is a positive integer greater than 1, and the image information of the 1 st stage is the image information during initial water supply. In addition, the invention also discloses a monitoring method of the device. The invention greatly reduces the workload in the seed germination rate monitoring process and the error caused by the change of the environment in the prior art, realizes the traceability of data and has constant test environment.

Description

Monitoring device and monitoring method for seed germination test
Technical Field
The invention relates to the technical field of crop seed research, in particular to a monitoring device and a monitoring method for a seed germination test.
Background
Seeds are the basis of grains, and monitoring of seed quality is an important aspect in agricultural work. Seeds must be tested for germination prior to harvest, storage, deployment and sowing. The germination rate of seeds is a very important indicator. Whether new variety breeding or research on seed vitality, seed aging and the like requires the seed germination rate as an evaluation index. Through the measurement to the seed germination percentage, can avoid using the seed that the germination percentage is low to sow and lead to growing seedlings the failure and cause the loss of manpower, material resources, financial resources.
At present, in the traditional method for monitoring the germination rate of seeds, a certain amount of seeds need to be placed in a germination box for cultivation, the germination state of the seeds is regularly observed and the germination amount is recorded by a manual counting method, and further the germination rate of the seeds is obtained by calculation through data statistics, so that the method has the following problems: firstly, in the process that the worker repeatedly observes the seeds, the germination form of the seeds is identified by the worker, no data image and other retention information exist, the data cannot be traced back, and the worker has long operation time and wastes time and labor; secondly, the seeds need to be taken out of the germination box in the observation process of the staff, the temperature and the humidity of the seed culture are changed, the long-time observation process is not beneficial to the constancy of the test environment, and the test error is brought by the change of the environment.
In view of the above, how to solve the problems of the prior art, such as the irretraceable data, the long operation time and the inconstant test environment during the monitoring process of the germination rate of the seeds, is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a monitoring device and a monitoring method for a seed germination test, so as to reduce the large calculation error in the seed germination rate monitoring process in the prior art, realize data traceability, further reduce the operation time and solve the problem of constant test environment.
In order to achieve the purpose, the technical scheme of the invention is as follows: the invention provides a monitoring device for a seed germination test, which comprises:
the germination device unit comprises a box body, a germination dish and a water supply assembly, wherein the germination dish is arranged in the box body and used for culturing seeds, and the water supply assembly is used for supplying water into the germination dish;
the image acquisition unit is used for periodically acquiring the image information of the seeds in the germination dish and sending the image information;
and the control unit is electrically connected with the image acquisition unit and used for receiving and storing the image information, comparing the image information of the nth stage with the image information of the 1 st stage, and obtaining the germination information of the nth stage through comparison and calculation, wherein n is a positive integer larger than 1, and the image information of the 1 st stage is the image information during initial water supply.
Further, the image acquisition unit comprises a camera, the camera is arranged above the germination dish, and the camera is used for periodically acquiring the image information of the seeds in the germination dish and sending the image information;
the control unit comprises a control module, a storage module and a data analysis module, the control module is electrically connected with the camera, the storage module is used for receiving and storing the image information, the data analysis module extracts the image information from the storage module and compares and calculates the germination information, and the germination information comprises the germination quantity.
Further, the number of the germination dishes is a plurality,
the image acquisition unit still include with control module electric connection's biax linkage subassembly, the biax linkage subassembly sets up the box is inside and be located a plurality of the top of the ware sprouts, install on the biax linkage subassembly the camera, control module is used for control the biax linkage subassembly moves so that along the horizontal direction the camera is to a plurality of the ware one-to-one of sprouting gathers image information.
Furthermore, the water supply assembly comprises a water supply tank, a water supply main pipe and water supply branch pipes, the water supply main pipe is used for communicating the water supply tank with the water supply branch pipes, the water supply branch pipes are arranged in parallel, one ends of the water supply branch pipes, far away from the water supply main pipe, are arranged vertically upwards, the inner side walls, far away from one ends of the water supply branch pipes, extend towards the center to form first plugging rings, the inner side walls in the middle of the water supply branch pipes extend towards the center to form second plugging rings, floating balls are arranged in the water supply branch pipes, the floating balls are movably arranged between the first plugging rings and the second plugging rings, the floating balls are in clearance fit with the inner side walls of the water supply branch pipes, the density of the floating balls is smaller than that of water, and the floating balls are used for plugging the water supply branch pipes when being abutted against the first plugging rings;
the germination dish is including the culture tank and the chamber of fetching water that are linked together, the bottom plate upper surface of culture tank with the bottom plate upper surface parallel and level in chamber of fetching water, culture tank upper end opening, the culture tank is used for cultivateing the seed, the bottom plate in chamber of fetching water is provided with the intake, the intake with the outside wall looks adaptation of water supply branch pipe, be provided with the pole of fetching water in the chamber of fetching water, the upper end of pole of fetching water with the roof fixed connection in chamber of fetching water, the lower extreme orientation of pole of fetching water the intake, just the lower extreme of pole of fetching water and the bottom plate upper surface parallel and level in chamber of fetching water, water supply branch pipe runs through selectively the intake, makes the pole of fetching water pushes down the floater is in order to realize water supply branch pipe and chamber intercommunication of fetching water.
Furthermore, a first partition plate is arranged between the culture tank and the water taking cavity, the bottom plate of the culture tank and the bottom plate of the water taking cavity are integrally formed, and gaps are formed between the lower end of the first partition plate and the upper surface of the bottom plate of the culture tank and the upper surface of the bottom plate of the water taking cavity respectively.
Further, the culture tank is a cuboid;
still include with a plurality of lifting unit that water supply branch pipe one-to-one set up, lifting unit is used for driving culture tank with releasable mode drives culture tank reciprocates, lifting unit includes:
the fixing rods are fixed on the upper surface of the bottom plate of the box body and are respectively connected with the four corners of the culture tank in a sliding mode, the fixing rods are arranged in the vertical direction, and the caliper is fixedly connected with the fixing rods and is provided with a plurality of clamping teeth in the vertical direction;
the lateral wall of culture tank articulates there is joint spare, be provided with bayonet socket and the circular arc mouth of connecting in order on the joint spare, the bayonet socket selectively with one of them joint tooth joint cooperation, make during circular arc mouth and joint tooth sliding fit the bayonet socket breaks away from the joint tooth, the lateral wall fixedly connected with fixed block of culture tank, the fixed block is connected with reset spring's one end, reset spring's the other end with the joint spare is connected, reset spring is used for the drive the bayonet socket orientation the joint tooth motion.
Furthermore, a first groove is formed in the side wall, close to the culture tank, of the clamping piece, a sleeve is connected in the first groove in a sliding mode, a compression spring is arranged in the first groove, one end of the compression spring is connected with the first groove, the other end of the compression spring is connected with the sleeve, and the sleeve is made of ferromagnetic materials;
a second groove is formed in the outer side wall of the culture tank, the second groove is matched with the sleeve, and the second groove is formed in one side, away from the caliper, of the clamping piece;
the caliper is provided with return teeth, the return teeth are arranged at the upper ends of the clamping teeth, the return teeth protrude out of the clamping teeth along the direction towards the clamping piece, and the return teeth are in sliding fit with the arc openings to drive the clamping piece to rotate, so that the sleeve is simultaneously embedded in the first groove and the second groove under the driving of the compression spring, and the bayonet is in clearance fit with the clamping teeth;
the upper surface of the bottom plate of the box body is provided with a magnet, the culture tank slides downwards along the fixing rod, the second groove is opposite to the magnet, and the magnet can attract the sleeve to be separated from the second groove and contract into the first groove.
Compared with the prior art, the invention at least has the following advantages:
according to the invention, the seeds are placed in the germination dish, the germination dish is placed in the box body, and the image information of the seeds in the germination dish is regularly acquired and stored, so that the data has traceability; by comparing and calculating the image information, the automatic identification of the germination quantity of the seeds is realized, the information such as germination vigor and germination rate can be further obtained, the workload in the monitoring process of the germination rate of the seeds in the prior art is greatly reduced, and the problem of long operation time is solved; when image information is collected, the germination container does not need to be taken out of the box body, the test environment is guaranteed to be constant, and test errors caused by environment change are reduced.
The invention also provides a monitoring method of the monitoring device based on the seed germination test, which comprises the following steps:
placing the seeds on the germination dish in an interval mode;
supplying water to the germination dish by using the water supply assembly;
the control unit controls the image acquisition unit to periodically acquire the image information of the seeds in the germination dish, and the image acquisition unit sends the image information to the control unit;
the control unit receives and stores the image information, compares the image information of the nth stage with the image information of the 1 st stage, and determines germination information at the nth stage.
Further, the germination information includes a germination amount, and the germination amount of the seed in the mth germination dish is calculated by the control unit in the following manner:
acquiring the image information of the 1 st stage of the seeds in the mth germination dish;
dividing the image information of the seed at the 1 st stage in the mth germination dish into image blocks with the same quantity as the seeds according to the image information of the seed at the 1 st stage in the mth germination dish, ensuring that the image blocks are not overlapped, each image block contains one seed, and respectively obtaining the color information of each image block of the seed at the 1 st stage in the mth germination dish;
acquiring the image information of the nth stage of the seeds in the mth germination dish;
dividing the image information of the nth stage of the seed in the mth germination dish into the image blocks which are the same as the image information of the 1 st stage of the seed in the mth germination dish according to the image information of the nth stage of the seed in the mth germination dish, and respectively obtaining the color information of each image block of the nth stage of the seed in the mth germination dish;
comparing the color information of the image block of the nth stage in the mth germination dish with the color information of the image block of the 1 st stage in the mth germination dish in a one-to-one correspondence manner respectively, and counting the number of the image blocks with color difference;
the germination quantity of the seeds in the mth germination dish is the quantity of the image blocks with color differences;
wherein: m is a positive integer greater than or equal to 1.
Further, the germination information further comprises a germination proportion, and the calculation formula of the germination proportion is as follows: the germination proportion of the seeds in the mth germination dish = Mn/M × 100%, where Mn is the germination number of the seeds in the mth germination dish, and M is the total number of the seeds in the mth germination dish.
Compared with the prior art, the monitoring method of the seed germination test and the monitoring device of the seed germination test have the same advantages, and are not described again.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic diagram of the overall structure of a monitoring device for a seed germination test according to the present invention;
FIG. 2 is a schematic view of another direction of the monitoring device for germination test of seeds according to the present invention;
FIG. 3 is a schematic view of the present invention with the culture dish engaged with the lifting assembly;
FIG. 4 is a cross-sectional view of the boat in snap-fit engagement with the lift assembly of the present invention;
FIG. 5 is a schematic structural view of the engagement member of the present invention engaged with the caliper;
FIG. 6 is a schematic view of the present invention with the culture dish disengaged from the lifting assembly;
FIG. 7 is a block diagram of the connection between the control unit and the image acquisition unit according to the present invention.
Reference numerals: 1. a box body; 2. a germination dish; 3. a camera; 4. a control module; 5. a storage module; 6. a data analysis module; 7. a column; 8. a connecting rod; 9. a second separator; 10. a first motor; 11. a first fixing frame; 12. a second fixing frame; 13. a driving pulley; 14. a first lead screw; 15. a second screw rod; 16. a passive pulley; 17. a belt; 18. a third fixing frame; 19. a movable rod; 20. a slider; 21. a second motor; 22. a third screw rod; 23. a water supply tank; 24. a water main; 25. a water supply branch pipe; 26. a first plugging ring; 27. a second plugging ring; 28. a floating ball; 29. a culture tank; 30. a water taking cavity; 31. a water taking rod; 32. a light supplement lamp strip; 33. a first separator; 34. fixing the rod; 35. a caliper; 36. a clamping piece; 37. a fixed block; 38. a return spring; 39. a first groove; 40. a sleeve; 41. a compression spring; 42. a second groove; 43. clamping teeth; 44. returning teeth; 45. and a magnet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Referring to fig. 1-2 and fig. 7, the invention provides a monitoring device for a seed germination test, which comprises a germination device unit, an image acquisition unit and a control unit.
Wherein: the device unit that sprouts provides the culture environment for the seed, specifically includes box 1, the ware 2 and the water supply installation of sprouting, and the ware 2 and the water supply subassembly setting of sprouting is inside box 1, and the ware 2 that sprouts can be dismantled with box 1 and be connected, and the seed is put and is cultivateed in the ware 2 that sprouts, and the water supply subassembly is arranged in supplying water to the ware 2 that sprouts. The image acquisition unit is used for regularly acquiring the image information of the seeds in the germination dish 2 and sending the image information. The control unit is preferably a computer, and the control unit is electrically connected with the image acquisition unit and is used for receiving and storing image information, comparing the image information of the nth stage with the image information of the 1 st stage, and obtaining germination information in the nth stage through comparison and calculation, wherein n is a positive integer larger than 1, and the image information of the 1 st stage is the image information in the initial water supply.
When the germination container is used, the germination paper is laid in the germination container 2, the seeds are placed above the germination paper at intervals, no overlapping between the seeds is ensured, the germination container 2 is further placed in the box body 1, the water supply process of the germination container 2 is realized by being communicated with a water supply device, at the moment, the image information of the seeds in the germination container 2 is collected by the image collecting unit, the image information of the 1 st stage is obtained, the image information of the seeds in the germination container 2 is collected regularly at the later stage, the image information of the multiple stages is obtained, the time interval period for collecting the images is preferably 1 day, and can be 12 hours and the like. The image information of multiple stages can be obtained in the above manner, and the germination information at the nth stage is calculated by comparing the image information of the nth stage with the image information of the 1 st stage, and the germination information includes the germination quantity of the seeds at the nth stage in the germination dish 2, the germination proportion at the nth stage, and the like, so as to further obtain the information of germination vigor, germination rate, and the like.
It can be understood that the box body 1 in the invention can be a closed device, and components such as the light supplement lamp strip 32 and a heater (not shown in the figure) are arranged in the box body 1, so as to realize a constant test monitoring environment, and components such as the light supplement lamp strip 32 and the heater are also not arranged in the box body 1, so that the box body 1 is exposed in a constant test monitoring room for operation. Thereby ensuring that the seeds are always kept in a constant monitoring environment in the germination test process.
In an embodiment of the present invention, the box body 1 is designed to be transparent, and the germination dish 2 is provided with a light supplement lamp strip 32 for simulating natural illumination in the germination process of seeds.
According to the invention, the seeds are placed in the germination dish 2, the germination dish 2 is placed in the box body 1, and the image information of the seeds in the germination dish 2 is regularly acquired and stored, so that the data have traceability; by comparing and calculating the image information, the automatic identification of the germination quantity of the seeds is realized, the information such as germination vigor and germination rate can be further obtained, and the problems of large manual calculation error and long operation time are solved; when image information is collected, the germination dish 2 does not need to be taken out of the box body 1, and the constancy of the test environment is guaranteed.
Preferably, the image acquisition unit comprises a camera 3, the camera 3 preferably adopts a CCD camera, the image information of the seeds in the germination dish 2 is acquired by means of fixing the height and the focal length, the camera 3 is arranged above the germination dish 2, and the camera 3 is used for periodically acquiring the images of the seeds in the germination dish 2 and sending the image information; the control unit comprises a control module 4, a storage module 5 and a data analysis module 6, the control module 4 is electrically connected with the camera 3, the control module 4 controls the camera 3 to carry out a shooting process so as to realize the whole process of image acquisition, the storage module 5 is used for receiving and storing image information, and the data analysis module 6 extracts the image information from the storage module 5 and obtains germination information through comparison and calculation; the germination information includes the germination quantity and the germination proportion.
The germination quantity is calculated by the following method:
acquiring image information of the 1 st stage of seeds in a germination dish 2;
dividing the image information of the seed at the 1 st stage in the germination dish 2 into image blocks with the same quantity as the seeds according to the image information of the seed at the 1 st stage in the germination dish 2, ensuring that the image blocks are not overlapped, wherein each image block contains 1 seed, and respectively obtaining the color information of each image block of the seed at the 1 st stage in the germination dish 2;
acquiring image information of the nth stage of seeds in the germination dish 2;
dividing the image information of the nth stage of the seeds in the germination dish 2 into image blocks which are the same as the image information of the 1 st stage of the seeds in the germination dish 2 according to the image information of the nth stage of the seeds in the germination dish 2, and respectively obtaining the color information of each image block of the nth stage of the seeds in the germination dish 2;
comparing the color information of the image block of the nth stage in the germination dish 2 with the color information of the image block of the 1 st stage in the germination dish 2 in a one-to-one correspondence manner respectively, and counting the number of the image blocks with color difference;
the number of seeds germinated in the germination dish 2 is the number of image blocks with color difference.
The formula for calculating the germination ratio is as follows:
Figure BDA0003984249140000111
wherein Mn is the germination quantity of the seeds in the nth stage in the germination dish 2, and M is the total quantity of the seeds in the germination dish 2.
In order to achieve a simultaneous monitoring of the test process for the simultaneous cultivation of a plurality of germination dishes 2. The germination dishes 2 are arranged in a plurality of numbers, the image acquisition unit further comprises a double-shaft linkage assembly, the double-shaft linkage assembly is electrically connected with the control module 4, the double-shaft linkage assembly is arranged in the box body 1 and located above the germination dishes 2, the camera 3 is arranged on the double-shaft linkage assembly, and the control module 4 is used for controlling the double-shaft linkage assembly to move in the horizontal direction so that the camera 3 can acquire images of the germination dishes 2 one by one.
Specifically, a plurality of germination dishes 2 are distributed in a rectangular array along the front-back direction and the left-right direction of the box body 1; be provided with stand 7 around the dish 2 that sprouts, the top of stand 7 is provided with connecting rod 8, and connecting rod 8 is used for connecting two adjacent stands 7, fixedly connected with second baffle 9 and third mount 18 on the connecting rod 8, and second baffle 9 sets up 1 top right sides of box, and third mount 18 sets up on the left of box top.
A first motor 10 and a first fixing frame 11 are fixedly connected above the front end of the second partition plate 9, an output shaft of the first motor 10 is coaxially and fixedly connected with a driving belt wheel 13 and a first screw rod 14, and the first screw rod 14 is rotatably connected with the first fixing frame 11; a second fixing frame 12 is fixed above the rear end of the second partition plate 9, the second fixing frame 12 is connected with a second lead screw 15 in a rotating mode, a driven belt wheel 16 is fixedly connected with the second lead screw 15 in a coaxial mode, the driven belt wheel 16 is in transmission connection with a driving belt wheel 13 through a belt 17, threads of the first lead screw 14 and the second lead screw 15 are same in rotating direction and extend along the left-right direction, a third fixing frame 18 is respectively connected with the first lead screw 14 and the second lead screw 15 in a rotating mode, a movable rod 19 is in threaded connection with the first lead screw 14 and the second lead screw 15, a sliding groove is formed in the movable rod 19, a sliding block 20 is connected in the sliding groove in a sliding mode, a camera 3 is installed on the lower surface of the sliding block 20, a second motor 21 is installed on the movable rod 19, a third lead screw 22 is connected with an output shaft of the second motor 21 in a coaxial transmission mode, the third lead screw 22 is arranged along the front-back direction, and the third lead screw 22 is in threaded connection with the sliding block. The first motor 10 and the second motor 21 are electrically connected to the control module 4, respectively.
When the device is used, the control module 4 controls the first motor 10 to be started, so that the first screw rod 14 and the second screw rod 15 synchronously rotate, and the movable rod 19 drives the sliding block 20 to move along the left-right direction; control module 4 through the control of second motor 21 and open for the rotation of third lead screw 22 can realize that slider 20 removes along the fore-and-aft direction, can realize slider 20 through above-mentioned mode and remove along left right direction or fore-and-aft direction, and then drive camera 3 and remove along left right direction or fore-and-aft direction, further realize the image acquisition process to a plurality of dishes 2 that sprout.
Preferably, the water supply assembly of the present invention includes a water supply tank 23, a water supply trunk 24, and water supply branch pipes 25, wherein one end of the water supply trunk 24 is communicated with the water supply tank 23, the water supply trunk 24 is also communicated with the plurality of water supply branch pipes 25, the plurality of water supply branch pipes 25 are arranged in parallel, and one ends of the water supply branch pipes 25, which are far from the water supply trunk 24, are arranged vertically upward. The water supply tank 23, the water supply trunk pipe 24 and the water supply branch pipe 25 constitute a communicating vessel, i.e., water is injected into the water supply tank 23, and the liquid levels of the water supply tank 23 and the water supply branch pipe 25 are maintained to be flat when the water is relatively stationary. The inside wall of the water supply branch pipe 25, which is far away from one end of the water supply main pipe 24, extends to the center to form a first plugging ring 26, the inside wall of the middle part of the water supply branch pipe 25 extends to the center to form a second plugging ring 27, a floating ball 28 is arranged in the water supply branch pipe 25, the floating ball 28 is in clearance fit with the water supply branch pipe 25, the floating ball 28 is arranged between the first plugging ring 26 and the second plugging ring 27, the diameter of the floating ball 28 is larger than the inner diameters of the first plugging ring 26 and the second plugging ring 27, the density of the floating ball 28 is smaller than that of water, and the floating ball 28 is used for plugging the water supply branch pipe 25 when being abutted against the first plugging ring 26.
Referring to fig. 3-6 in combination, the germination dish 2 comprises a culture tank 29 and a water intake cavity 30 which are communicated with each other, the upper surface of the bottom plate of the culture tank 29 is flush with the upper surface of the bottom plate of the water intake cavity 30, the upper end of the culture tank 29 is open, the bottom plate of the water intake cavity 30 is provided with a water intake, the water intake is matched with the outer side wall of the water supply branch pipe 25, a water intake rod 31 is arranged in the water intake cavity 30, the upper end of the water intake rod 31 is fixedly connected with the top plate of the water intake cavity 30, the lower end of the water intake rod 31 faces the water intake, and the lower end of the water intake rod 31 is flush with the upper surface of the bottom plate of the water intake cavity 30. The branch water supply pipe 25 selectively penetrates through the water intake port, so that the water intake rod 31 presses the floating ball 28 to communicate the branch water supply pipe 25 with the water intake chamber 30. In order to improve the sealing property when the intake port is engaged with the outer wall of the water branch pipe 25, a sealing ring (not shown) may be attached to the side wall of the intake port.
Through injecting water into the water supply tank 23, the height of one end of the water supply branch pipe 25, which is far away from the water supply main pipe 24, is lower than the water surface height in the water supply tank 23, at the moment, the floating ball 28 is abutted against the first blocking ring 26, the water supply branch pipe 25 is blocked, and by the method, when the culture tank 29 is not used, the water taking cavity 30 is not contacted with the water supply branch pipe 25, at the moment, the water supply branch pipe 25 does not flow out, namely, the water in the water supply branch pipe 25 is supplied into the culture tank 29 by the water taking cavity 30 only when the water taking cavity 30 is matched with the water supply branch pipe 25 for use.
In actual use, the water intake of the water intake cavity 30 faces the end of the water supply branch pipe 25 far away from the water main pipe 24, the end of the water supply branch pipe 25 far away from the water main pipe 24 is inserted into the water intake, and the lower end of the water intake rod 31 presses the floating ball 28 downwards to make the floating ball far away from the first blocking ring 26, so that the water is ensured to enter the water intake cavity 30 and further enter the culture tank 29 by the water supply branch pipe 24.
Preferably, a first partition plate 33 is arranged between the culture tank 29 and the water taking cavity 30, the bottom plate of the culture tank 29 and the bottom plate of the water taking cavity 30 are integrally formed, and gaps are respectively arranged between the lower end of the first partition plate 33 and the upper surface of the bottom plate of the culture tank 29 and the upper surface of the bottom plate of the water taking cavity 30, so that the flow rate of water entering the culture tank 29 from the water taking cavity 30 can be reduced. The design can prevent the seeds from being washed due to overlarge water flow when water enters the tank culture tank 29 from the water taking cavity 30, so that the arrangement of the seeds is disordered, and the analysis process of the image information at the later stage is influenced.
Preferably, the culture tank 29 of the present invention is a rectangular parallelepiped. The present invention further includes a plurality of lifting members provided in one-to-one correspondence with the water supply branch pipes 25, the lifting members being adapted to drive the culture tank 29 to move up and down in a releasable manner. The lifting component can adjust the height of the water supply branch pipe 25 inserted into the water taking cavity 30, further change the liquid level height of the water in the culture tank 29, and realize the determination process of germination tests of the same batch of seeds under different water depths. Specifically, the height of the water supply branch pipe 25 inserted into the water intake chamber 30 is defined as a first distance, the difference between the water level of the water storage tank and the height of the water supply branch pipe 25 at the end away from the water supply main pipe 24 is defined as a second distance, and the water level in the culture tank 29 is equal to the sum of the first distance and the second distance. In order to ensure that the liquid level of the water in the culture tank 29 is kept stable, the liquid level in the water supply tank 23 should be kept stable, for example, the liquid level in the water supply tank 23 is monitored by using a water level meter, and when the liquid level in the water supply tank 23 drops, water is timely supplemented, which belongs to the prior art and is not described herein again.
Optionally, the lifting assembly includes a fixing rod 34 and a caliper 35.
Wherein the fixing rods 34 are arranged along the vertical direction, the fixing rods 34 are fixed on the upper surface of the bottom plate of the box body 1, the number of the fixing rods 34 is four, and the four fixing rods 34 are respectively arranged at the four corners of the culture tank 29 and are in sliding connection with the four corners of the culture tank 29. The caliper 35 is fixedly connected with the fixing rod 34, and the caliper 35 is provided with a plurality of clamping teeth 43 along the vertical direction.
The lateral wall of culture tank 29 articulates there is joint piece 36, be provided with bayonet socket and the circular arc mouth of connecting in order on the joint piece 36, bayonet socket and circular arc mouth set up towards joint tooth 43, the bayonet socket selectively with one of them joint tooth 43 joint cooperation, the circular arc mouth makes the bayonet socket break away from joint tooth 43 with joint tooth 43 sliding fit, the lateral wall fixedly connected with fixed block 37 of culture tank 29, fixed block 37 is connected with reset spring 38's one end, reset spring 38's the other end is connected with joint piece 36, reset spring 38 is used for driving the bayonet socket towards joint tooth 43 motion.
Optionally, a first groove 39 is formed in a side wall of the clamping member 36 close to the culture tank 29, a sleeve 40 is slidably connected to the first groove 39, a compression spring 41 is arranged in the first groove 39, one end of the compression spring 41 is connected to the first groove 39, the other end of the compression spring 41 is connected to the sleeve 40, and the sleeve 40 is made of ferromagnetic material; the outer side wall of the culture tank 29 is provided with a second groove 42, the depth of the second groove 42 is smaller than the length of the sleeve 40, the second groove 42 is matched with the sleeve 40, and the second groove 42 is arranged on one side, away from the caliper 35, of the clamping piece 36.
The caliper 35 is provided with a return tooth 44, the return tooth 44 is arranged at the upper ends of the plurality of clamping teeth 43, the return tooth 44 protrudes out of the clamping teeth 43 along the direction towards the clamping piece 36, and the clamping piece 36 is driven to rotate when the return tooth 44 is in sliding fit with the arc opening, so that the sleeve 40 is embedded in the second groove 42 and the clamping opening is in clearance fit with the clamping teeth 43; the upper surface of the bottom plate of the case 1 is provided with a magnet 45, and the cultivation tank 29 is slid down along the fixing bar 34 so that the second recess 42 is opposed to the magnet 45, and the magnet 45 can attract the sleeve 40 to come out of the second recess 42 and retract into the first recess 39.
When the device is used, a water intake of the water intake cavity 30 faces one end of the water supply branch pipe 25, which is far away from the water supply main pipe 24, the sleeve 40 is embedded into the first groove 39 and the second groove 42 at the same time, the culture tank 29 is pressed downwards, the magnet 45 is opposite to the second groove 42, the magnet 45 attracts the sleeve 40 to be separated from the second groove 42 and embedded into the first groove 39, the clamping piece 36 rotates towards the caliper 35 under the action of the return spring 38, the bayonet is matched and abutted with the clamping teeth 43 with different heights by lifting the culture tank 29, the adjustment process of the height of the culture tank 29 is realized, and the liquid level height of water in the culture tank 29 is adjusted; after the experiment, mention culture tank 29 upwards, order about joint spare 36 to rotate when return tooth 44 and circular arc mouth sliding fit for first recess 39 is relative with second recess 42, and sleeve 40 inlays simultaneously under compression spring 41's effect and establishes in first recess 39 and second recess 42, prevents joint spare 36 rotatory, and the separation process that culture tank 29 and slide caliper rule 35 can be realized to further upwards proposing culture tank 29.
The invention also discloses a monitoring method of the monitoring device based on the seed germination test, which comprises the following steps:
placing the seeds on a germination dish at intervals, preferably radiating germination paper in the germination dish, and placing the seeds in the germination dish in a rectangular array mode;
supplying water to the germination container by using the water supply assembly;
the control unit controls the image acquisition unit to periodically acquire image information of seeds in the germination dish, and the image acquisition unit sends the image information to the control unit;
the control unit receives and stores the image information, compares the image information of the nth stage with the image information of the 1 st stage, and determines the germination information at the nth stage.
Further, the germination information comprises the germination quantity, and the germination quantity of the seeds in the mth germination dish is calculated by the control unit in the following way:
acquiring the image information of the 1 st stage of seeds in the mth germination container;
dividing the image information of the seed at the 1 st stage in the mth germination dish into image blocks with the same quantity as the seeds according to the image information of the seed at the 1 st stage in the mth germination dish, ensuring that the image blocks are not overlapped, wherein each image block contains one seed, and respectively obtaining the color information of each image block of the seed at the 1 st stage in the mth germination dish;
acquiring image information of the nth stage of seeds in the mth germination dish;
dividing the image information of the nth stage of the seeds in the mth germination dish into image blocks which are the same as the image information of the 1 st stage of the seeds in the mth germination dish according to the image information of the nth stage of the seeds in the mth germination dish, and respectively obtaining the color information of each image block of the nth stage of the seeds in the mth germination dish;
comparing the color information of the image block of the nth stage in the mth germination dish with the color information of the image block of the 1 st stage in the mth germination dish in a one-to-one correspondence manner respectively, and counting the number of the image blocks with color difference;
the germination quantity of the seeds in the mth germination dish is the quantity of the image blocks with color difference; wherein: m is a positive integer greater than or equal to 1.
Further, the germination information also comprises a germination proportion, and the calculation formula of the germination proportion is as follows: the germination proportion of the seeds in the mth germination dish = Mn/M × 100%, where Mn is the germination number of the seeds in the mth germination dish, and M is the total number of the seeds in the mth germination dish.
Compared with the prior art, the monitoring method of the seed germination test and the monitoring device of the seed germination test have the same advantages, and are not described again.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. A device for monitoring germination testing of seeds, comprising:
the germination device unit comprises a box body (1), a germination dish (2) arranged in the box body (1) and a water supply assembly, wherein the germination dish (2) is used for culturing seeds, and the water supply assembly is used for supplying water into the germination dish (2);
the image acquisition unit is used for periodically acquiring the image information of the seeds in the germination dish (2) and sending the image information;
and the control unit is electrically connected with the image acquisition unit and used for receiving and storing the image information, comparing the image information of the nth stage with the image information of the 1 st stage, and obtaining the germination information of the nth stage through comparison and calculation, wherein n is a positive integer larger than 1, and the image information of the 1 st stage is the image information during initial water supply.
2. The device for monitoring seed germination tests according to claim 1, characterized in that the image acquisition unit comprises a camera (3), the camera (3) being arranged above the germination dish (2), the camera (3) being configured to periodically acquire and send image information of the seeds in the germination dish (2);
the control unit comprises a control module (4), a storage module (5) and a data analysis module (6), the control module (4) is electrically connected with the camera (3), the storage module (5) is used for receiving and storing the image information, the data analysis module (6) extracts the image information from the storage module (5) and compares and calculates the germination information, and the germination information comprises the germination quantity.
3. The device for monitoring a germination test of seeds according to claim 2, wherein the germination dish (2) is in a plurality,
the image acquisition unit still include with control module (4) electric connection's biax linkage subassembly, biax linkage subassembly sets up box (1) is inside and be located a plurality of the top of sprouting ware (2), install on the biax linkage subassembly camera (3), control module (4) are used for control the biax linkage subassembly moves so that along the horizontal direction camera (3) are to a plurality of sprouting ware (2) are gathered one by one image information.
4. The device for monitoring the seed germination test according to claim 3, wherein the water supply assembly comprises a water supply tank (23), a water supply trunk (24) and a plurality of water supply branch pipes (25), the water supply trunk (24) is used for communicating the water supply tank (23) with the plurality of water supply branch pipes (25), the plurality of water supply branch pipes (25) are arranged in parallel, one end of each water supply branch pipe (25) away from the water supply trunk (24) is arranged vertically and upwards, the inner side wall of one end of each water supply branch pipe (25) away from the water supply trunk (24) extends towards the center to form a first blocking ring (26), the inner side wall of the middle part of each water supply branch pipe (25) extends towards the center to form a second blocking ring (27), a floating ball (28) is arranged in each water supply branch pipe (25), the floating ball (28) is movably arranged between the first blocking ring (26) and the second blocking ring (27), the floating ball (28) is in clearance fit with the inner side wall of each water supply branch pipe (25), the floating ball (28) has a density smaller than that the floating ball (28) abuts against the first blocking ring (26) to block the water supply branch pipes (25);
the germination dish (2) comprises a culture tank (29) and a water taking cavity (30) which are communicated, the upper surface of a bottom plate of the culture tank (29) is flush with the upper surface of the bottom plate of the water taking cavity (30), the upper end of the culture tank (29) is open, the culture tank (29) is used for culturing seeds, the bottom plate of the water taking cavity (30) is provided with a water taking opening, the water taking opening is matched with the outer side wall of the water supply branch pipe (25), a water taking rod (31) is arranged in the water taking cavity (30), the upper end of the water taking rod (31) is fixedly connected with a top plate of the water taking cavity (30), the lower end of the water taking rod (31) faces the water taking opening, the lower end of the water taking rod (31) is flush with the upper surface of the bottom plate of the water taking cavity (30), the water supply branch pipe (25) selectively penetrates through the water taking opening, and the water taking rod (31) presses the floating ball (28) downwards to achieve communication between the water supply branch pipe (25) and the water taking cavity (30).
5. The device for monitoring seed germination test according to claim 4, wherein a first partition (33) is disposed between the cultivation tank (29) and the water intake chamber (30), the bottom plate of the cultivation tank (29) and the bottom plate of the water intake chamber (30) are integrally formed, and a gap is disposed between the lower end of the first partition (33) and the upper surface of the bottom plate of the cultivation tank (29) and the upper surface of the bottom plate of the water intake chamber (30).
6. The device for monitoring seed germination test according to claim 4, wherein the culture tank (29) is a rectangular parallelepiped;
the culture tank is characterized by further comprising a plurality of lifting components which are arranged in one-to-one correspondence with the water supply branch pipes (25), the lifting components are used for driving the culture tank (29) to drive the culture tank (29) to move up and down in a releasable mode, and the lifting components comprise:
the fixing device comprises four fixing rods (34) which are fixed on the upper surface of a bottom plate of the box body (1) and are respectively connected with four corners of the culture tank (29) in a sliding mode, and calipers (35) which are fixedly connected with the fixing rods (34), wherein the calipers (35) are provided with a plurality of clamping teeth (43) in the vertical direction;
the lateral wall of culture tank (29) articulates there is joint spare (36), be provided with bayonet socket and the circular arc mouth that connects in order on joint spare (36), the bayonet socket selectively with one of them joint tooth (43) joint cooperation, make during circular arc mouth and joint tooth (43) sliding fit the bayonet socket breaks away from joint tooth (43), the lateral wall fixedly connected with fixed block (37) of culture tank (29), fixed block (37) are connected with the one end of reset spring (38), the other end of reset spring (38) with joint spare (36) are connected, reset spring (38) are used for the drive the bayonet socket orientation joint tooth (43) motion.
7. The device for monitoring the seed germination test according to claim 6, wherein a first groove (39) is arranged on the side wall of the clamping member (36) close to the culture tank (29), a sleeve (40) is slidably connected to the first groove (39), a compression spring (41) is arranged in the first groove (39), one end of the compression spring (41) is connected with the first groove (39), the other end of the compression spring (41) is connected with the sleeve (40), and the sleeve (40) is made of ferromagnetic material;
a second groove (42) is formed in the outer side wall of the culture tank (29), the second groove (42) is matched with the sleeve (40), and the second groove (42) is formed in one side, away from the caliper (35), of the clamping piece (36);
the caliper (35) is provided with return teeth (44), the return teeth (44) are arranged at the upper ends of the clamping teeth (43), the return teeth (44) protrude out of the clamping teeth (43) along the direction towards the clamping piece (36), and the return teeth (44) drive the clamping piece (36) to rotate when being in sliding fit with the arc opening, so that the sleeve (40) is simultaneously embedded in the first groove (39) and the second groove (42) under the driving of the compression spring (41), and the clamping opening is in clearance fit with the clamping teeth (43);
the upper surface of the bottom plate of the box body (1) is provided with a magnet (45), the culture tank (29) slides downwards along the fixing rod (34) and the second groove (42) is opposite to the magnet (45), and the magnet (45) can attract the sleeve (40) to be separated from the second groove (42) and contract into the first groove (39).
8. A method for monitoring a device for monitoring germination of seeds according to any one of claims 1 to 7, comprising the steps of:
placing the seeds on the germination dish (2) in an interval manner;
the water supply assembly is used for supplying water to the germination dish (2);
the control unit controls the image acquisition unit to periodically acquire the image information of the seeds in the germination dish (2), and the image acquisition unit sends the image information to the control unit;
the control unit receives and stores the image information, compares the image information of the nth stage with the image information of the 1 st stage, and determines germination information at the nth stage.
9. The monitoring method according to claim 8, wherein the germination information comprises a germination number, the germination number of the seeds in the mth germination dish (2) being calculated by a control unit by:
acquiring the image information of the 1 st stage of the seeds in the mth germination dish (2);
dividing the image information of the seed at the 1 st stage in the mth germination dish (2) into image blocks with the same number as the seed according to the image information of the seed at the 1 st stage in the mth germination dish (2), ensuring that the image blocks do not overlap, and each image block contains 1 seed, and respectively obtaining the color information of each image block of the seed at the 1 st stage in the mth germination dish (2);
acquiring the image information of the nth stage of the seeds in the mth germination dish (2);
dividing the image information of the nth stage of the seeds in the mth germination dish (2) into the same image blocks as the image information of the 1 st stage of the seeds in the mth germination dish (2) according to the image information of the nth stage of the seeds in the mth germination dish (2), and respectively acquiring the color information of each image block of the nth stage of the seeds in the mth germination dish (2);
comparing the color information of the image block of the nth stage in the mth germination dish (2) with the color information of the image block of the 1 st stage in the mth germination dish (2) in a one-to-one correspondence manner respectively, and counting the number of the image blocks with color difference;
the germination number of the seeds in the mth germination dish (2) is the number of the image blocks with color differences;
wherein: m is a positive integer greater than or equal to 1.
10. The monitoring method according to claim 9, wherein the germination information further includes a germination percentage, and the germination percentage is calculated by the formula: the m-th germination dish (2)
Figure FDA0003984249130000061
Wherein Mn is the germination number of the seeds in the mth germination dish (2), and M is the total number of the seeds in the mth germination dish (2). />
CN202211559908.7A 2022-12-06 2022-12-06 Monitoring device and monitoring method for seed germination test Pending CN115943773A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116137990A (en) * 2023-04-19 2023-05-23 黑龙江省农业科学院绥化分院 Detection device for germination capacity of rice seeds
CN118402353A (en) * 2024-07-02 2024-07-30 龙门实验室 Intelligent seeder seeding monitoring device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116137990A (en) * 2023-04-19 2023-05-23 黑龙江省农业科学院绥化分院 Detection device for germination capacity of rice seeds
CN118402353A (en) * 2024-07-02 2024-07-30 龙门实验室 Intelligent seeder seeding monitoring device

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