WO2021082655A1

WO2021082655A1 - Rhizo-box for acquiring crop phenotype

Info

Publication number: WO2021082655A1
Application number: PCT/CN2020/110635
Authority: WO
Inventors: 傅秀清; 姜东�; 丁艳锋; 吴劼; 周国栋; 毛江美
Original assignee: 南京慧瞳作物表型组学研究院有限公司
Priority date: 2019-10-28
Filing date: 2020-08-21
Publication date: 2021-05-06
Also published as: CN110617768A

Abstract

A rhizo-box for acquiring a crop phenotype. A support frame, supporting a side barrier plate (42) and light transmitting plates (4), is formed from a rhizo-box frame (3) and a base plate. The support frame connects the side barrier plate (42) and the light transmitting plates (4) to form a root system accommodation space having a flat cuboid structure. A top portion of the rhizo-box is further provided with an upper end cover (2). The aerial portion of a crop plant grows from a through hole in the middle of the upper end cover (2), and a root system of the underground portion thereof grows in the root system accommodation space. Since the light transmitting plates (4) have a limiting function at the front and back sides, the root system grows close to inner side walls of the light transmitting plates (4), such that detailed structural characteristics of the root system are directly observable through the light transmitting plates (4). The invention enables, by means of the light transmitting plates (4), direct acquisition of images containing underground phenotype characteristics of the crop plant by using an ordinary image acquisition apparatus after a light shielding plate outside the rhizo-box is removed. The invention achieves a simple and convenient acquisition process, involves a low hardware cost, and does not affect crop growth, thereby facilitating periodic tracking of an underground phenotype characteristic change condition of a crop plant during the entire growth cycle thereof.

Description

Root box for obtaining crop phenotype

Technical field

The present invention relates to the technical field of crop phenotype analysis, in particular to a root box for obtaining crop phenotype.

Background technique

In order to cultivate good crop varieties, it is necessary to continuously measure the changes in the phenotypic characteristics and physiological parameters caused by the growth of the organs during the growth of the crops. At present, the traditional artificial climate chamber has the function of cultivating crops, and its function of measuring phenotype mainly relies on manual observation and measurement to describe the external characteristics of crops, so as to obtain the relationship between genotype, environmental factors and crop phenotype. This work often relies on manual detection of individual traits of small samples of plants, so the amount of data is limited, the efficiency is low, and it is difficult to carry out comprehensive analysis of multiple traits of plants. Moreover, because it requires manual detection, human factors are introduced, which can easily lead to measurement data. The error. With the rapid development of plant genomics research and molecular breeding, there is an urgent need for high-throughput, high-precision and low-cost phenotypic analysis devices to meet the requirements of plant growth, yield, quality, and tolerance to biotic and abiotic stresses. Demand for relevant phenotypic data.

The root system acts as a water-absorbing organ, a salt-absorbing organ, and supports the plant. Its phenotype is one of the indicators that most directly reflects the phenotypic traits of crops and the level of breeding.

The existing surface type can only be detected by CT. In CT mode, the small structures in the root system, such as capillary roots, cannot be known and cannot be detected. And the crop cultivation environment is not suitable for CT equipment.

Other methods, such as nail plate method, container method, root washing method, and other traditional root system phenotyping methods, are destructive and time-consuming and labor-intensive on crop root systems. The recording and measurement data of these traditional measurement methods are prone to errors and low efficiency. The commonly used electronic computed tomography (CT) and magnetic resonance imaging (MRI) technologies can detect the more obvious root structure and reconstruct objects, but they are expensive and inefficient, and the X-rays they use can affect the human body. And plants produce certain radiation damage.

Summary of the invention

In view of the shortcomings of the prior art, the present invention provides a root box for obtaining the phenotype of crops. The present invention uses a flat root box to make the crop roots grow close to the edge of the root box without cleaning the roots of the crops, and through simple shooting Means can obtain accurate root phenotype data. The present invention specifically adopts the following technical solutions.

First of all, in order to achieve the above objective, a root box for obtaining crop phenotypes is proposed, which includes: a root box skeleton having a plurality of uprights, and a bottom plate connecting the bottom ends of each of the uprights, the uprights and the bottom plate A root accommodating space formed in a flat rectangular parallelepiped structure for accommodating the roots of the crops; side baffles, which are arranged on the left and right sides of the root accommodating space, and are fixedly connected to the uprights; a light-transmitting plate, which is arranged on the roots The front and rear sides of the accommodating space are connected with the uprights, and cooperate with the uprights and the side baffles to close the root accommodating space; a light-shielding plate, which is close to the light-transmitting plate, is arranged at each The outer side of the plate is detachably connected with each column in the root accommodating space; the upper end cover of the root box is fixedly connected to the upper end of each column, and the middle of the upper end cover of the root box is reserved for accommodating crop growth. The distance between the light-transmitting plates in the flat rectangular parallelepiped structure of the hole ranges from 10 mm to 20 mm.

Optionally, the above-mentioned root box for obtaining crop phenotype, wherein the width of the side baffle does not exceed 10 mm, and the width of the light-transmitting plate exceeds 10 mm.

Optionally, the above-mentioned root box for obtaining crop phenotypes, wherein the vertical columns on the front and rear sides of the root accommodating space are respectively provided with first chutes parallel to the axis of the vertical column, The edges on both sides of the light-transmitting plate are respectively inserted into the first sliding grooves on the surfaces of two adjacent uprights, and the light-transmitting plate moves downward along the first sliding grooves to the front edge or the front edge of the bottom plate. The rear edge abuts.

Optionally, the above-mentioned root box for obtaining crop phenotypes, wherein the vertical columns on the front and rear sides of the root accommodating space are respectively provided with first mounting grooves parallel to the axis of the vertical column, A magnetic stripe is arranged in the first installation slot, and the magnetic stripe is interference-clamped into the first installation slot; at least the edge of the light-shielding plate is provided with a magnetically conductive material, and the magnetically conductive material is made of The magnetic strip is attracted and fixed on the surface of the column, and is shielded from the outer side of the light-transmitting plate.

Optionally, the above-mentioned root box for obtaining crop phenotype, wherein the outer edge of the upper end cover of the root box protrudes from the plane where the light-shielding plate is located, and is fixed on the upper end surface of the upright column by screws; The through hole in the middle of the upper end cover of the root box is rectangular, and a root box cover plate is embedded in the rectangular through hole, and a center hole is arranged in the middle of the root box cover plate for accommodating crop growth.

Optionally, the above-mentioned root box for obtaining a crop phenotype, wherein the root box is fixed by a root box frame, the root box frame is a long plate structure, and a plurality of root boxes are arranged along the length direction of the long plate structure. There are two root box installation grooves, each of the root boxes passes through each of the root box installation grooves, and the upper surface of the root box installation groove is abutted by the lower edge of the upper end cover of the root box to fix each of the root boxes in Under the long board structure.

Optionally, the above-mentioned root box for obtaining crop phenotype, wherein the length direction of each root box installation groove is parallel to the length direction of the long plate structure, and the light-transmitting plate and the light-shielding plate of the root box They are all arranged along the long side of the long board structure.

Optionally, the above-mentioned root box for obtaining a crop phenotype, wherein the two ends of the root box frame are respectively provided with a handle, and a groove structure is provided under the handle to fix the root box frame.

Optionally, the above-mentioned root box for obtaining crop phenotype, wherein, when cultivating crops, the root box frame is set on the crop cultivation frame, and the object cultivation frame is provided with a fixed rod, and the fixed rod clamp It is connected to the groove structure under the root box rack to support and fix each of the root boxes; when the crop phenotype is obtained, the root box rack is removed from the crop cultivation rack and placed on the root box fixing rack. The upper end of the root box fixing frame is clamped with the groove structure to fix the root box, the light-shielding plate of each root box is disengaged from the magnetic stripe, and the sampling device penetrates the light transmission of each root box The board shoots the root structure of the crop inside the root box.

Optionally, the above-mentioned root box for obtaining a crop phenotype, wherein the crop cultivation rack is arranged in a multi-layer structure, and a plurality of root boxes parallel to each other are arranged in each layer of the crop cultivation rack. The top layer of the crop cultivation rack and each layer of the structure are respectively provided with partitions, the middle of the partition is arranged with spray nozzles for spraying moisture and nutrients to the crops below, the length direction of the partition A plurality of fill light lamps are evenly arranged on the upper surface to provide light. The upper surface of the partition is set to be convex on all sides and concave and flat in the middle, and the upper surface of the partition is used to collect the water sprayed from the upper layer. And nutrition.

Beneficial effect

In the present invention, a bracket for supporting the side baffle and the light-transmitting plate is formed by the root box skeleton and the bottom plate, and the bracket is connected with the side baffle and the light-transmitting plate to form a root accommodating space with a flat rectangular parallelepiped structure. The top of the root box is also provided with an upper end cap of the root box. The aboveground part of the crop grows from the through hole in the middle of the upper end cap of the root box. The root system of the underground part grows in the root containing space. Due to the limitation of the light-transmitting plates on the front and rear sides, It grows close to the inner wall of the light-transmitting plate, and the detailed characteristics of the root structure can be directly observed through the light-transmitting plate. As a result, the present invention can conveniently remove the light-shielding plate outside the root box, pass the light-transmitting plate, and directly obtain an image containing the morphological features of the crop ground through ordinary image acquisition equipment for analysis. The acquisition process is simple and convenient, the hardware cost is low, and the growth of the crop is not affected, and it can conveniently track the change of the ground surface morphological characteristics of the crop regularly during the full growth cycle.

Further, in order to avoid the influence of light on the morphological characteristics of the crop ground, the present invention further provides a detachable structure for the shading plate. In order to facilitate the acquisition of the phenotype, the shading plate can be specifically attracted by the magnetic force of the magnetic strip to realize a detachable connection between the shading plate and the support structure. When obtaining the crop phenotype, fix the root box holder on the root box holder through the groove structure on the lower surface of the root box, and remove the light-shielding plate. In this way, the light-transmitting plates on both sides of the root box can be directly Shows the ground surface typographic characteristics of the crop. In this way, the image acquisition device can be directly translated from both sides of the root box frame along a straight line, and the ground surface features of the crop can be directly collected.

Further, in order to better realize the cultivation of crops, the root box fixing frame can be arranged on a crop cultivation frame equipped with nozzles, supplementary lights, and sensing equipment. Sprinklers and supplementary lights provide nutrients and light for crops. Sensing equipment can detect nutrient data obtained by crops in real time, and record them through the data processing system to realize the tracking of crop cultivation data and the phenotypic characteristics it exhibits, and record both The association between the kinds of data.

Other features and advantages of the present invention will be described in the following description, and partly become obvious from the description, or understood by implementing the present invention.

Description of the drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and together with the embodiments of the present invention, are used to explain the present invention, and do not constitute a limitation to the present invention. In the attached picture:

Fig. 1 is a schematic diagram of the assembly process of the root box for obtaining crop phenotype according to the present invention;

Figure 2 is a schematic diagram of the installation method between the root box and the root box rack of the present invention;

Figure 3 is an overall schematic diagram of the clapboard structure in the crop cultivation rack provided by the root box rack of the present invention;

Figure 4 is a schematic diagram of the overall structure of the root box rack in the present invention;

Figure 5 is a partial schematic diagram of the crop cultivation rack set on the root box rack of the present invention;

Figure 6 is a schematic diagram of the root box rack set on the root box fixing rack in the present invention;

Figure 7 is an exploded view of the overall system for crop cultivation and phenotype acquisition;

Figure 8 shows the flash structure at the bottom of the open slot.

In the figure, 1 represents the cover of the root box; 2 represents the upper end cover of the root box; 3 represents the skeleton of the root box; 4 represents the light-transmitting plate; 41 represents the knurled structure of the light-shielding plate; 42 represents the side baffle; 5 represents the magnetic strip; 6 Represents the partition; 61 represents the fill light; 62 represents the nozzle; 63 represents the connector; 7 represents the root box rack; 71 represents the handle; 72 represents the groove structure; 8 represents the crop cultivation rack; 81 represents the fixed rod; 82 represents the L-shaped On the board, 83 represents the load cell; 9 represents the root box holder.

Detailed ways

In order to make the objectives and technical solutions of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be described clearly and completely in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

Those skilled in the art can understand that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meanings as those commonly understood by those of ordinary skill in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with the meanings in the context of the prior art, and unless defined as here, they will not be used in idealized or overly formal meanings. Explanation.

In the present invention, the meaning of "and/or" means that each exists alone or both exist simultaneously.

The meaning of "inside and outside" in the present invention refers to the root box itself, the direction pointing to the root system of the crop contained in the root box is inside, and vice versa; it does not refer to the device mechanism of the present invention. The specific limit.

The meaning of "left and right" in the present invention means that when the user is facing the direction of the light-transmitting plate, the user's left is the left, and the user's right is the right, instead of the device mechanism of the present invention. Specific restrictions.

The meaning of "connection" in the present invention can be a direct connection between components or an indirect connection between components through other components.

The meaning of "up and down" in the present invention means that when the user is facing the light-transmitting plate, the direction from the bottom plate to the upper end cover of the root box is up, and vice versa, it is down, not the device mechanism of the present invention. The specific limit.

The meaning of "front and back" in the present invention means that when the user is facing the light-transmitting panel, the front of the user is the front, and vice versa, it is the back, rather than a specific limitation on the device mechanism of the present invention.

Fig. 1 is a device for obtaining high-throughput, high-precision and low-cost crop phenotypes according to the present invention, which is specifically configured as a flat root box. The root box includes:

The root box skeleton 3 has a plurality of uprights and a bottom plate connected to the bottom end of each of the uprights. The uprights and the bottom plate form a root accommodating space with a flat rectangular parallelepiped structure to accommodate the roots of crops;

Side baffles 42, which are arranged on the left and right sides of the root system accommodating space, and are fixedly connected to the uprights;

A light-transmitting plate 4, which is arranged on the front and back sides of the root accommodating space, is connected to the upright post, and cooperates with the upright post and the side baffle to close the root accommodating space;

A light-shielding plate, which is close to the light-transmitting plate 4, is arranged on the outer side of each of the light-transmitting plates 4, and is detachably connected to each column in the root system accommodating space;

The upper end cover 2 of the root box is fixedly connected to the upper end of each of the uprights, and the middle part of the upper end cover 2 of the root box is reserved with a through hole for accommodating the growth of crops;

Wherein, the distance between the light-transmitting plates in the flat cuboid root accommodating space structure ranges from 10 mm to 20 mm, which can compress the thickness dimension of the root box accommodating crop roots to 10 mm. As a result, the crop root system grows close to the edge of the root box, facilitating side shots. More subtle phenotypic features that cannot be captured by CT imaging technology can be obtained during shooting. When shooting, only the sensor with its own light source is used to reduce the influence of light on the acquisition of root phenotype. Therefore, the present invention can solve the problem of the inability to carry out accurate and automatic acquisition and analysis of crop phenotypes in the existing climate chamber.

In order to realize the supply of nutrients and light to the crops inside the root box, the root box can be further arranged on the root box rack 7 shown in FIG. 2, and the root box rack 7 fixes each root box uniformly as shown in FIG. 4的物养架8 On the shelf.

Specifically, referring to FIG. 2, the above-mentioned root box rack 7 is a long plate structure, and a plurality of root box installation grooves are provided along the length direction of the long plate structure, and each of the root boxes is installed through each of the root boxes. In the groove, the lower edge of the upper end cover 2 of the root box abuts against the upper surface of the root box installation groove, and each of the root boxes is fixed under the long plate structure. In order to facilitate the image acquisition equipment to extract the root phenotypic characteristics inside the light-transmitting plate, the length direction of each root box installation slot is set parallel to the length direction of the long plate structure, and the light-transmitting plate 4 of the root box Both the light-shielding plate and the light-shielding plate are arranged along the long side of the long plate structure to facilitate the direct shooting from the side of the root box frame 7 to obtain the crop phenotype.

When the crop phenotype is obtained, the root box frame 7 is removed from the crop cultivation frame 8, and placed on the root box fixing frame 9 as shown in FIG. 6, and the shading plate of each root box is separated from the root box frame The connection relationship between the columns. The sampling device passes through the light-transmitting plate 4 of each root box and moves sequentially from both sides of the long side of the root box frame 7 to respectively photograph the root structure of the crops inside each root box.

In the process of cultivating crops, the root box rack 7 can be uniformly fixed to each root box in the object cultivation rack 8 shown in FIG. 4. As shown in FIG. 4, the object cultivation rack 8 is set to In a multi-layer structure, each layer of the crop cultivation rack 8 is arranged with a plurality of root box racks 7 parallel to each other; the top layer of the crop cultivation rack 8 and the structure of each layer can be further respectively provided with In the partition 6 shown in FIG. 3, a nozzle 62 is arranged in the middle of the partition 6 for spraying moisture and nutrients to the crops below, and a plurality of supplementary lights are evenly arranged in the length direction of the partition 6 The lamp 61 is used to provide light. Referring to the partial schematic diagram shown in FIG. 5, the upper surface of the partition 6 can be set to be convex around and flat in the middle, and the upper surface of the partition 6 is used to collect water and nutrients sprayed from the upper layer.

Therefore, the root box of the present invention can be used for both the cultivation of crops and the extraction of ground morphology. There is no need to manipulate the crops during the extraction process, which can protect the integrity of the crop roots, obtain the detailed characteristics of the crop roots, and also During the complete cycle of crop growth, the phenotype and nutritional data of each crop are tracked, and data samples in a wider dimension are obtained.

In a more specific implementation manner, the present invention can be applied to an overall system for crop cultivation and phenotype acquisition similar to that shown in FIG. 7. The system includes control and display area I, crop cultivation area II, phenotype acquisition area III, and environmental equipment area IV. one of them:

The crop cultivation area II is provided with an electric sliding door connected to the control and display area I and the phenotype acquisition area III. One of the electric sliding doors is made of transparent glass material and is installed between the control and analysis area I and the crop cultivation area II, which is convenient for personnel to enter and exit the crop cultivation area II and observe the conditions in the crop cultivation area II in the control and analysis area I ；

The electric sliding door of the phenotype acquisition area III is made of opaque material and is installed between the crop cultivation area II and the phenotype acquisition area III to facilitate the entry and exit of the phenotype acquisition area III. After the door is closed, the crop phenotype acquisition can be guaranteed When there is no other ambient light in the entire phenotype acquisition area III that affects the normal operation of the top view graph acquisition sensor group and the side view phenotype acquisition sensor group, it is convenient for the processing and analysis of the phenotype data. The phenotype acquisition sensor group can be realized by image acquisition equipment such as a camera.

A crop cultivation rack 8 is provided in the crop cultivation area II, which includes a partition, a root box, a root box rack, a nutrient solution tank with a pump, a water pipe, a spray head, a fill light, an environmental sensor group and other structures. Among them, the nutrient solution tank with pump is installed at the bottom of the crop cultivation rack, and the climate chamber control and analysis system controls the nutrient solution contained in the nutrient solution tank with pump to transport the nutrient solution to the crop cultivation through the water pipe in the crop cultivation rack 8. In each layer of the shelf, the spray nozzle in the shelf sprays the crops under the shelf.

The sprinkler head and the light supplement lamp are installed under the partition and can be used to provide nutrient solution and light necessary for the growth of crops. The above-mentioned crop cultivation area II and phenotype acquisition area III can be uniformly set as a climate chamber controlled by the environmental equipment area IV, which has a climate chamber control and analysis system, which can adjust the amount of nutrient solution sprayed by the spray nozzle in real time according to the needs of crop cultivation And the light intensity of the fill light.

In the crop cultivation rack 8 of the crop cultivation area II, the partitions can be installed between each layer structure of the crop cultivation rack. Multi-layer partitions can be installed according to the quantity requirements of the cultivated crops, and each layer of the partitions has The upper surface can be set to have a convex structure on all sides and a concave and flat structure in the middle to facilitate the collection of excess nutrient solution sprayed by the spray nozzle in the upper crop growth area.

Both ends of the root box frame 7 are respectively provided with handles 71, and a groove structure 72 is provided under the handle 71 to fix the root box frame 7. For the crop cultivation rack 8 shown in FIG. 5, the root box rack 7 is arranged on the crop cultivation rack 8, and the material cultivation rack 8 is provided with a fixed rod 81 which is clamped into the The groove structure 72 under the root box rack 7 supports and fixes each of the root boxes. For the type acquisition area III shown in FIG. 6, when the phenotype of the crop is acquired, the root box rack 7 is removed from the crop cultivation rack 8 and placed on the root box fixing rack 9. The upper end clamps the groove structure 72 to fix the root box to prevent the root box from shaking back and forth and to support the root box. During the sampling process, the light-shielding plate of each root box is separated from the magnetic strip 5, and the sampling device photographs the root structure of the crop inside the root box through the light-transmitting plate 4 of each root box. The root box fixing frame 9 can be referred to as shown in FIG. 6 or FIG. 7 and is installed on the upper surface of the seedbed by fastening bolts for placing the root box frame. The root box fixing frame 9 can be set to the corresponding interval according to the data requirements of obtaining the crop top view chart type, and multiple root box fixing frames are installed to support the multiple root box frames, and the system and the side view chart are obtained through the top view chart type. The type acquisition system separately acquires the multi-angle chart type data of multiple groups of crops.

An L-shaped plate 82 connecting the vertical pillars of the crop growing frame 8 shown in FIG. 5 is arranged below the fixed rod 81 of the crop growing frame 8. One side of the L-shaped plate 82 is connected to the upper surface of the other side of the vertical pillar. Set the weighing sensor 83. The root box frame 7 is installed on the upper part of the load cell by screw connection, and is used to support the root box. The fixed rod 81 set above the load cell can be installed with multiple root box racks 7 according to the requirements of the number of cultivated crops. The load cell is installed on the L-shaped plate by screws, and is used to monitor the weight change of the root box frame 7 in real time, obtain the supply amount of nutrient solution according to the weight change, and transmit it to the control and analysis system for corresponding data recording. The L-shaped plate is installed on the crop cultivation rack by screws, and is used to support the load cell, the root box and the root box rack.

In a more specific implementation manner, the root box is composed of an upper end cover of the root box, a root box skeleton, a light-transmitting plate, a light-shielding plate, a side baffle, a magnetic strip and a screw as shown in FIG. 1. The root box is placed in the rectangular hole of the root box frame, and the outer size of the upper end cap of the root box is larger than the shape size of the rectangular hole on the root box frame, which is convenient for the root box to be placed on the upper surface of the root box frame; the root box can provide the necessary crop growth The hydroponic and soil culture environment, its rectangular shape and the characteristics of light transmission inside and shading outside are convenient for the cultivation of roots, stems and leaves of crops and the extraction of phenotypes.

In order to obtain more characteristic information in the bottom surface of the crop, the width of the side baffle 42 is set to not exceed 10 mm, and the width of the light-transmitting plate 4 exceeds 10 mm, which is restricted on the front and rear sides of the crop. The root system grows, and the root system of the crop grows close to the light-transmitting plate, so that it can be easily acquired by general image acquisition equipment.

The root box skeleton can be made by 3D printing technology. There are two card slots on the inner side of each frame, a total of eight card slots. The upper surface of the bottom can also have four additional slots to facilitate the installation and removal of the light-transmitting plate and the lower edge of the side baffle. There can be two card slots on the outer side of the front of each frame, four card slots in total, which are used for interference fit with the magnetic stripe.

Wherein, the grooves on the uprights on the front and back sides of the root accommodating space correspond to first sliding grooves parallel to the axis of the upright, and the edges on both sides of the light-transmitting plate 4 are respectively inserted into two adjacent ones. Root the first chute on the surface of the column, the light-transmitting plate 4 moves downward along the first chute to abut against the front edge or the rear edge of the bottom plate. The light-transmitting plate and the side baffle plate are installed on the root box skeleton along the inner groove of each frame of the root box skeleton and the groove on the upper surface of the bottom. The phenotype of the root system can be obtained through the light-transmitting plate.

The grooves on the front and back sides of the vertical column of the root accommodating space correspond to a first mounting groove parallel to the axis of the vertical column, and a magnetic strip 5 is provided in the first mounting groove. The interference of the strip 5 is inserted into the first installation slot.

At least the edge of the light-shielding plate is set with a magnetic conductive material, or the whole can be made of iron sheet or other opaque magnetic conductive material. The magnetic conductive material is attracted by the magnetic strip 5 and fixed on the surface of the column. The said magnetic strips can be arranged in four of the outer card slots on the front of each frame respectively, and the light-shielding plate is attracted to the magnetic strips by magnetic force to realize the light-shielding effect.

In order to facilitate the fixing of the root box on the root box frame to realize unified transportation and fixation, the outer edge of the upper end cover 2 of the root box protrudes from the plane where the light-shielding plate is located, and is fixed on the upper end surface of the upright column by screws. The root box is placed in the rectangular hole of the root box rack, and the outer size of the upper end cap of the root box is larger than the outer size of the rectangular hole on the root box rack, which is convenient for the root box to be placed on the upper surface of the root box rack; the root box can provide crops The hydroponic and soil culture environment is necessary for growth, and the rectangular shape and the characteristics of light transmission and shading facilitate the cultivation of the roots, stems and leaves of crops and the extraction of phenotypes. The through hole in the middle of the upper end cover 2 of the root box can be specifically configured as a rectangle, and a root box cover plate 1 is embedded in the rectangular through hole, and a center hole or a tapered hole is provided in the middle of the root box cover plate 1. , For accommodating crop growth.

During cultivation, the seeds of the crop or seeds with germinated roots can be placed in the tapered hole of the root box cover plate 1. The tapered hole can fix the relative position of the crop, and it is convenient for the crop to be displayed by fixing the position of the crop. Type of automatic acquisition;

The root box cover 1 is installed in the opening groove of the upper end cover of the root box through a plurality of convex structures on the side. The several convex structures on the side can ensure that the root box cover 1 is stuck in the opening groove of the upper end cover of the root box. In Figure 8, the flash structure at the bottom of the opening groove of the upper end cover of the root box can ensure that the root box cover 1 will not be pressed into the root box due to excessive external force during the installation of the root box cover 1;

The light-shielding plate can also be selected to be inserted and installed on the upright post of the root box frame along the inner side of each frame of the root box frame and the snap groove on the bottom upper surface, so as to block light transmission.

When the root box is placed in the crop cultivation area, the shading plate is in the installed state, which is convenient to block the ambient light and reduce the impact of ambient light on the crop root system; when the root box is placed in the phenotype acquisition area, the shading plate can be sucked or pulled out with a magnet The card slot is set to facilitate the acquisition of the root phenotype.

When the root box is placed in the crop cultivation area, the shading plate is in the installed state, which is convenient to block the ambient light and reduce the impact of ambient light on the crop root system; when the root box is placed in the phenotype acquisition area, the shading plate can be taken out to facilitate the root system table Type of acquisition;

A knurled structure 42 can also be provided on the surface of the shading plate, which is convenient for the operator to install and disassemble the shading plate according to usage requirements.

Therefore, through the design of the crop root box, the present invention can facilitate the cultivation of crops, and can simultaneously perform high-throughput, high-precision, and low-cost crop phenotype acquisition and analysis functions on the crops. It uses the environmental control in the environmental cabin to simultaneously carry out high-throughput, high-precision, and low-cost crop phenotype acquisition and acquisition of crop stems, leaves and other aboveground organs and roots and other underground organs under the influence of different environmental factors. analysis.

The above are only the embodiments of the present invention, and the description is relatively specific and detailed, but it should not be understood as a limitation to the patent scope of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and these all fall within the protection scope of the present invention.

Claims

A root box for obtaining crop phenotype, which is characterized in that it comprises:

A root box skeleton (3), which has a plurality of uprights, and a bottom plate connecting the bottom ends of each of the uprights, and the uprights and the bottom plate form a root accommodating space in a flat rectangular parallelepiped structure to accommodate the roots of crops;

Side baffles (42), which are arranged on the left and right sides of the root system accommodating space, and are fixedly connected to the uprights;

A light-transmitting plate (4), which is arranged on the front and back sides of the root accommodating space, is connected to the upright post, and cooperates with the upright post and the side baffle to close the root accommodating space;

A light-shielding plate, which is close to the light-transmitting plate (4), is arranged on the outer side of each of the light-transmitting plates (4), and is detachably connected with each column in the root system accommodation space;

The upper end cover (2) of the root box is fixedly connected to the upper end of each of the uprights, and the middle part of the upper end cover (2) of the root box is provided with a through hole for accommodating the growth of crops

The distance between the light-transmitting plates in the flat rectangular parallelepiped structure ranges from 10 mm to 20 mm.
The root box for obtaining crop phenotype according to claim 1, wherein the width of the side baffle (42) does not exceed 10 mm, and the width of the light-transmitting plate (4) exceeds 10 mm.
The root box for obtaining crop phenotype according to claim 1-2, characterized in that the columns on the front and back sides of the root accommodating space are respectively provided with a line parallel to the axis of the column. The first sliding groove, the two side edges of the light-transmitting plate (4) are respectively inserted into the first sliding grooves on the surfaces of two adjacent uprights, and the light-transmitting plate (4) is downward along the first sliding groove Move to abut against the front edge or the rear edge of the bottom plate;
The root box for obtaining crop phenotype according to claim 3, characterized in that, on the uprights on the front and back sides of the root accommodating space are further provided with a first parallel to the axis of the upright. An installation slot, a magnetic strip (5) is arranged in the first installation slot, and the magnetic strip (5) is interference-fitted into the first installation slot;

At least the edge of the light-shielding plate is provided with a magnetically conductive material, and the magnetically conductive material is attracted by the magnetic strip (5), fixed on the surface of the column, and shielded on the outside of the light-transmitting plate (4).
The root box for obtaining crop phenotype according to claim 1, wherein the outer edge of the upper end cover (2) of the root box protrudes from the plane where the light-shielding plate is located, and is fixed to the upright post by screws The upper end surface; the through hole in the middle of the upper end cover of the root box (2) is rectangular, and the root box cover plate (1) is embedded in the rectangular through hole, and the root box cover plate (1) is set in the middle There is a center hole for accommodating crop growth.
The root box for obtaining crop phenotype according to claim 1-5, wherein the root box is fixed by a root box frame (7), and the root box frame (7) is a long plate structure, A plurality of root box installation grooves are provided in the length direction of the long plate structure, each of the root boxes passes through each of the root box installation grooves, and the lower edge of the upper end cover (2) of the root box abuts against the root box The upper surface of the installation groove fixes each of the root boxes under the long plate structure.
The root box for obtaining crop phenotype according to claim 6, wherein the length direction of each root box installation groove is parallel to the length direction of the long plate structure, and the light-transmitting plate of the root box (4) and the shading plate are arranged along the long side of the long plate structure.
The root box for obtaining crop phenotype according to claim 7, characterized in that, both ends of the root box frame (7) are further provided with handles (71) respectively, and the handles (71) are provided below A groove structure (72) is provided to fix the root box frame (7).
The root box for obtaining crop phenotype according to claim 8, wherein:

When cultivating crops, the root box frame (7) is arranged on the crop cultivation frame (8), and the object cultivation frame (8) is provided with a fixed rod (81), and the fixed rod is clamped into the root box The groove structure (72) under the frame (7) supports and fixes each of the root boxes;

When the crop phenotype is obtained, the root box frame (7) is removed from the crop cultivation frame (8), and placed on the root box fixing frame (9), and the upper end of the root box fixing frame (9) is clamped in place The groove structure (72) fixes the root box, the light-shielding plate of each root box is separated from the magnetic strip (5), and the sampling device penetrates the light-transmitting plate (4) of each root box Take pictures of the root structure of the crop inside the root box.
The root box for obtaining crop phenotypes according to claims 1-8, wherein the crop cultivation rack (8) is arranged in a multi-layer structure, and each layer of the crop cultivation rack (8) A plurality of root box racks (7) parallel to each other are arranged respectively; the top layer of the crop cultivation rack (8) and the partitions (6) between each layer structure are respectively arranged, and the partitions (6) A spray head (62) is arranged in the middle for spraying moisture and nutrients to the crops below, and the partition (6) is also evenly arranged with a plurality of fill lights (61) in the length direction for providing light. The upper surface of the partition (6) is set to be convex around and flat in the middle, and the upper surface of the partition (6) is used to collect the water and nutrients sprayed from the upper layer.