CN111239340B - Multichannel acquisition system and installation method for field crop root phenotype - Google Patents
Multichannel acquisition system and installation method for field crop root phenotype Download PDFInfo
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Abstract
The invention provides a multichannel acquisition system and an installation method for field crop root phenotype. The invention drives the monitor to horizontally run along the underground of the root growth area of the crop root system, thereby obtaining the distribution condition of the crop root system within 360 degrees along each root pipe in an endoscopic shooting mode. The invention can acquire and analyze the root phenotype of the field crops through the multi-channel root canal monitoring system in the state closest to nature, and solves the problems that the prior root canal monitoring equipment can not carry out field large-scale experiments and can not carry out accurate and automatic acquisition and analysis of the root phenotype of the crops.
Description
Technical Field
The invention relates to the technical field of field crop cultivation, in particular to a multichannel acquisition system and an installation method for field crop root phenotype.
Background
Crop phenotype is the result of some or all of the identifiable physical, physiological, and biochemical characteristics and traits resulting from the interaction of genes with the environment. The plant growth regulator comprises the structure, the composition and the growth and development process of crops, and not only reflects the expression regulation and control on the molecular level, but also reflects the complex traits of the plants such as physiology, biochemistry, morphological anatomy, stress resistance and the like.
The development of functional genomics and genetic technology in the field of crop breeding is the most convenient and effective means for increasing the yield of grains. Phenotype is the external expression of a crop gene, and is the result of the co-action of the crop's own gene and the external environment. Therefore, it becomes important to explore the relationship between crop genotypes, environmental factors and crop phenotypic characteristics and traits.
The plant root system is an important component of the plant and has very important functions, such as absorption and transportation of water and nutrients, storage of organic matters, plant anchoring, interaction with soil and the like. The development condition of the plant root system is important for research work of a plurality of plants, and is related to a series of processes such as selection of optimal treatment time of the plants, consistency of growth and development states of the plants before treatment, and timely feedback of response of the plant root system in the treatment process. Therefore, the collection and analysis of root phenotypic traits have become the key and difficult point of biological and phenotypic omics research. Due to the restriction of soil invisibility, the core of root phenotype collection is how to observe root growth in situ.
Traditional root system research work often relies on the individual characters of artifical manual detection small sample plant roots, therefore the data volume is limited, and is inefficient, is difficult to carry out the comprehensive analysis of plant roots multiple characters, and introduces human factor easily, very easily leads to the error of measured data. The existing root phenotype acquisition means has the defects of small sample analysis scale, high cost, time and labor waste, lack of normalization and low measurement precision, and becomes a bottleneck for restricting the plant genome function analysis and molecular breeding development. With the rapid development of plant genomics research and molecular breeding, a root phenotype analysis device with high throughput, high precision and low cost is urgently needed to meet the requirement of acquiring phenotype data related to plant growth, yield, quality, tolerance to biotic and abiotic stresses and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a multichannel acquisition system and an installation method for field crop root phenotype. The invention specifically adopts the following technical scheme.
To achieve the above object, a multi-channel acquisition system for field crop root phenotype is provided, which comprises: the root system detection channels comprise at least two root system detection channels which are embedded in parallel at the edge of a crop root system growth area, and the depth of the root system detection channels embedded underground at least reaches the growth depth range of the crop root system; the glass window is arranged on the side wall, close to the crop root, of the root system detection channel, and the root system of the crop at the edge of the crop root system growing area grows close to the glass window; root canals which are arranged between the root system detection channels which are parallel to each other, wherein the root canals penetrate through a crop root system growing area; the monitor, it sets up in each root canal, along the root canal in the regional underground horizontal operation of crop root system growth, the monitor is in along the rotation in a circumferential direction of root canal in the root canal, shoot the distribution situation of each root canal 360 within range crop root system along the line.
Optionally, the multichannel of above-mentioned arbitrary field crop root system phenotype obtains the system, wherein, root system test channel, its top is provided with the daylighting well, the daylighting well is transparent material, provides the illumination in introducing the root system test channel with natural light.
Optionally, the multichannel of above-mentioned arbitrary field crop root system phenotype acquires the system, wherein, still be provided with the book curtain structure on the glass window, it is put down by glass window top to roll up the curtain structure, shelters from the light that jets into in the crop root system growth area, perhaps upwards curls up the natural light that is introduced by the lighting well or for the lighting apparatus in the root system detection channel shoots and provides the illumination for the root system when shooting crop root system distribution situation on the glass window.
Optionally, the multichannel acquisition system of above-mentioned arbitrary field crop root phenotype, wherein, the root canal is equallyd divide including the multiunit that is parallel to each other interval setting in the crop root growth region, and every is equallyd divide in a set of and respectively includes the level setting many of the different degree of depth in the crop root growth region, and is parallel to each other between each root canal, and it respectively is provided with the monitor to equallyd divide in each root canal, and each monitor is respectively horizontal migration in the root canal and along the rotation in a circumferential direction of root canal shoots the distribution situation of each root canal 360 within range crop root system along the line.
Optionally, the multichannel of above-mentioned arbitrary field crop root system phenotype acquires the system, wherein, the root canal sets up to the array that N row M were listed as, and the interval equals between each row root canal, and it is the same that it buries the degree of depth in the crop root system growth area underground between each row root canal, and the deepest degree that the root canal was buried underground is no longer than the underground degree of depth of root system detection channel, each root canal is intraductal to divide equally and do not is provided with the monitor, and each monitor is in respectively horizontal migration and the rotation in a circumferential direction along the root canal in the root canal shoots the distribution situation of each root canal 360 within range crop roots along the line.
Optionally, the multichannel acquisition system for root phenotype of field crops as described in any of the above, wherein the root canals are arranged in an array of 5 rows and 5 columns, wherein the spacing between each row of root canals is greater than the spacing between each column of root canals.
Optionally, the multichannel acquisition system for the root phenotype of any field crop is characterized in that each root canal comprises a plurality of transparent tubes connected to form a straight line, the head end and the tail end of each transparent tube are respectively provided with a connecting part, the connecting parts of adjacent transparent tubes are mutually spliced and fixed or screwed and fixed, the ends of the transparent tubes at the head end and the tail end of each root canal are respectively provided with a sealing cover, and the sealing covers are made of opaque materials and shield light rays emitted into the root canals from the ends.
Optionally, in any one of the above multichannel acquiring systems for field crop root phenotype, the monitor main body is of a cylindrical structure, and the monitor is arranged inside a root tube formed by connecting the transparent tubes; one end or two ends of the monitor are provided with motion modules, the motion modules comprise rotating wheels which rotate along the circumferential direction of the cylindrical structure and driving wheels which rotate along the axial direction of the cylindrical structure, the monitor is driven by the driving wheels to horizontally move in the root canal along the axial direction of the root canal and is driven by the rotating wheels to rotate in the circumferential direction of the root canal; the LED light source is arranged in the middle of the monitor, and provides illumination required by shooting for the image acquisition unit in the monitor when the monitor shoots the distribution condition of the crop root system within the 360-degree range along the root canal, and at least illuminates the shooting area of the image acquisition unit; and the data transmission and storage module is arranged in the monitor and is electrically connected with the image acquisition unit, and the pictures of the distribution conditions of the crop roots at different angles, which are shot by the monitor at different positions in the root canal, are stored.
The invention also provides a method for installing any one of the field crop root phenotype multichannel acquisition systems, which comprises the following steps: firstly, fixing a perforating device in a prepared root system detection channel, and setting a guide drill rod of the perforating device to be aligned to the direction of installing a root canal in the root system detection channel on the other side; secondly, screwing and fixing the transparent pipe at the rear end of a guide drill rod of the perforating device, and fixing the transparent pipe on the perforating device, wherein a detection ring and a water outlet are arranged at the position of a drill bit at the front end of the guide drill rod; thirdly, supplying water to the drill bit through the water outlet, and simultaneously driving the guide drill rod to rotate to drill forward, wherein in the drilling process, a laser range finder arranged at the rear side of the drilling device is aligned with a detection ring arranged on the drill bit to correct the drilling direction; fourthly, when one section of transparent pipe is drilled into the crop root growth area, detaching the rear end of the transparent pipe from the punching device, screwing and fixing the front end of the next section of transparent pipe at the rear end of the transparent pipe drilled into the crop root growth area, and screwing and fixing the rear end of the next section of transparent pipe on the punching device; fifthly, repeating the third step and the fourth step until the first section of transparent pipe directly connected with the guide drill rod reaches the position for installing the root canal in the root system detection channel at the other side, disassembling the guide drill rod, and installing a sealing cover at the end part of the root canal formed by connecting the transparent pipes in the crop root system growth area; and sixthly, moving the perforating device to the installation position of the next pipe, and completing the installation of all the pipes according to the steps from the first step to the fifth step.
Based on the multichannel acquisition system, the invention also provides a multichannel acquisition method for the root phenotype of the field crops, which comprises the following steps: a1, arranging a monitor in each root canal, and recording the position of the monitor relative to the root canal; a2, turning on an LED light source in the monitor, driving a driving wheel of the monitor along the horizontal axial direction of the root canal, pushing the monitor to walk along the root canal to the opposite side, driving a rotating wheel of the monitor respectively when moving a shooting distance, pushing the monitor to rotate 360 degrees along the circumferential direction of the root canal, and shooting a picture of the distribution condition of the crop root system within the range of 360 degrees along the root canal in the rotating process; step a3, storing the pictures of the distribution status of the crop root system and the position of the monitor relative to the root canal when shooting, and splicing the pictures of the distribution status of the crop root system in the 360-degree range along the root canal obtained by each monitor into a complete picture of the distribution status of the crop root system at different depths according to the shooting sequence or the position sequence.
Advantageous effects
According to the invention, through the root system detection channels, the glass windows arranged on the root system detection channels, the root tubes arranged among the root system detection channels and the monitor, the monitor is driven to horizontally run along the underground of the root tubes in the growth area of the crop root system in an endoscopic mode, so that the distribution condition of the crop root system in a 360-degree range along each root tube is shot and obtained. The invention can acquire and analyze the root phenotype of the field crops through the multi-channel root canal monitoring system in the state closest to nature, and solves the problems that the prior root canal monitoring equipment can not carry out field large-scale experiments and can not carry out accurate and automatic acquisition and analysis of the root phenotype of the crops.
The root canal can be arranged to penetrate through the whole crop root growth area, so that the root phenotype of the root crop in the field can be acquired and analyzed at high flux and high precision by scanning the periphery of the root canal. Compared with the prior art, the method can improve the acquisition efficiency and the acquisition precision of the underground phenotype of the crop on the basis of not influencing the growth of the crop and ensuring the sampling accuracy.
The invention can further obtain the growth form information of the complete root system of the crop completely and intuitively through the glass window on the edge of the root system detection channel. This kind of mode can directly utilize current image scanning equipment directly to scan, directly extracts the phenotype data of crop root system, can also provide more audio-visual crop root system phenotype data for the researcher, and its data acquisition efficiency is higher, and can distinguish the plane that the root canal did belongs to, obtains the better sampling data of its precision from another visual angle of crop root system.
In order to avoid the influence of the external environment on the crop root system when the crop phenotype is acquired, the invention further arranges the sealing covers at the two ends of the root canal, and arranges the roller shutter structure on the glass window to shield the external illumination, thereby avoiding the influence of the external light on the natural growth environment of the crop root system and the influence on the accuracy of the phenotype expression.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of the overall architecture of the multi-channel acquisition system of the present invention for field crop root phenotype;
FIG. 2 is a schematic side view of a multi-channel acquisition system for field crop root phenotype of the present invention;
FIG. 3 is a schematic view of the root canal configuration in the multi-channel harvesting system of the present invention;
FIG. 4 is a schematic view of a mounting apparatus for a root canal structure in the multi-channel harvesting system of the present invention;
fig. 5 is a schematic view of a drill bit in the installation apparatus of a root canal structure in the multichannel acquisition system of the present invention.
In the drawings, 1 denotes a glass window; 2 denotes a root canal; 21 denotes a turntable; 22 denotes a small hydraulic support rod; 23 denotes a motor; 24 denotes a drain port; 25 denotes a detection ring; 3 denotes a producing well; 4 denotes a root detection channel; 5 denotes a slide guide; 6 denotes a monitor; 61 denotes an LED light source; 62 denotes a data place transfer memory module; 63 denotes a motion module; 71 denotes a stopper plate; 72 denotes a shock absorbing column; 73 denotes an L-shaped movable frame; 74 denotes an annular bracket; 75 denotes a support base; 76 represents a large hydraulic support rod; 77 denotes a laser range finder; 78 denotes a support plate; 79 denotes a valve; 791 denotes a connecting means.
Detailed Description
In order to make the purpose and technical solution of the embodiments of the present invention clearer, the technical solution of the embodiments of the present invention will be clearly and completely described below with reference to the drawings of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention without any inventive step, are within the scope of protection of the invention.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The meaning of "and/or" in the present invention means that the respective single or both of them exist individually or in combination.
The meaning of "inside and outside" in the present invention means that the direction pointing to the monitor inside the root canal is inside, and vice versa, with respect to the root canal itself; and not as a specific limitation on the mechanism of the device of the present invention.
The term "connected" as used herein may mean either a direct connection between the components or an indirect connection between the components via other components.
The meaning of "up and down" in the present invention means that, with respect to the multichannel acquisition system itself, the direction from the ground surface to the bottom of the sliding guide rail in the root system detection channel is downward, and vice versa, which is not a specific limitation on the mechanism of the apparatus of the present invention.
Referring to fig. 1, the present invention provides a multichannel acquisition system for field crop root phenotype, which is a multichannel monitoring system, and employs an endoscopic image acquisition device to dynamically and all-weather acquire data and images of soil moisture, temperature and crop root growth parameters of various plant roots in real time. Referring to fig. 2, the system specifically includes:
the root system detection channels 4 comprise at least two root system detection channels which are embedded in parallel at the edge of a crop root system growth area, and the depth of the root system detection channels 4 embedded underground at least reaches the growth depth range of the crop root system;
the glass window 1 is arranged on the side wall, close to the crop root, of the root system detection channel 4, and the root system of crops at the edge of a crop root system growing area grows close to the glass window 1;
the root canals 2 are arranged between the root system detection channels 4 which are parallel to each other, and the root canals 2 penetrate through a crop root system growing area;
monitor 6, it sets up in each root canal 2, along the root canal in the regional underground horizontal operation of crop root system growth, monitor 6 is in along the rotation in a circumferential direction of root canal in root canal 2, shoot the distribution situation of each root canal 360 within range crop root system along the line.
Root system test channel 4, its top is provided with daylighting well 3, daylighting well 3 is transparent material, introduces the natural light and provides the illumination in root system test channel 4. In order to avoid the influence of illumination light on the growth of the crop root system and the introduction of errors when the root system phenotype is shot, the invention can also correspondingly arrange a roller shutter structure on the glass window 1. The roller shutter structure can be put down by glass window 1 top at ordinary times, shelters from the light that penetrates into in the crop root system growth region, perhaps upwards curls up the natural light that is introduced by lighting well 3 or for the lighting apparatus in root system inspection channel 4 provides the illumination for the root system shooting when shooting crop root system distribution situation on glass window 1.
Root canal, it can set up to multistage formula transparent construction, its inside 360 degrees multi-level rotation type image monitor walking scanning of supplying shoots root canal around root canal and distributes, root canal tip stretches out to root system test channel 4 in, for avoiding light to influence crop root system around the root canal, still can further set up sealed lid at root canal tip.
The root canals may be arranged in the array configuration shown in fig. 1, for example, in a combination of 5 rows and 5 columns. The interval equals between each row of root canal, and it buries the degree of depth difference in crop root system growth region underground between each row of root canal, and the degree of depth difference that adjacent root canal was buried underground can set up to the phase difference the same, buries the ground of interval mutually parallel between the root canal and establishes in crop root system growth region. Wherein, its degree of depth of the deepest root canal that buries underground is not exceeded root system test channel 4 buries the degree of depth underground, and each root canal is intraductal equally divide and is provided with monitor 6 respectively, and each monitor 6 is in respectively root canal 2 interior horizontal migration and along the rotation in a circumferential direction of root canal shoot the distribution situation of each root canal 360 within ranges crop roots along the line.
The monitor 6 may be configured to have a cylindrical structure as shown in fig. 3 in order to obtain root structures of all crops at all angles in the whole root growth area of the crops. The monitor 6 is arranged at one end or two ends of the interior of the branch emitting tube 2 formed by connecting the transparent tubes, and a motion module 63 is arranged at the other end or two ends of the interior of the branch emitting tube to drive the monitor. Motion module 63, it includes along cylinder structure circumferential direction's rotation wheel, still includes along cylinder structure axial pivoted drive wheel, monitor 6 is in by the drive wheel drive horizontal migration in root canal 2, by the rotation wheel drive at the circumferential direction of root canal. The middle part of the monitor 6 is provided with an LED light source 61 which provides required lighting for the image acquisition unit in the monitor 6 when the monitor 6 shoots the distribution situation of the crop root system within 360 degrees along the root canal, and at least illuminates the shooting area of the image acquisition unit. And the data transmission and storage module 62 is arranged in the monitor 6 and is electrically connected with the image acquisition unit, and is used for storing the pictures of the distribution condition of the crop root systems at different angles, which are shot by the monitor 6 at different positions in the root canal. The shooting process can be carried out by a control unit in the monitor or an external remote control device according to the following modes:
a1, arranging the monitor 6 in each pipe 2 in the manner shown in fig. 3, and recording the position of the monitor relative to the pipe 2;
step a2, turning on an LED light source 61 in a monitor 6, driving a driving wheel of the monitor 6 along the horizontal axial direction of the root canal, pushing the monitor 6 to walk along the root canal to the opposite side, and driving a rotating wheel of the monitor 6 respectively every time the monitor moves a shooting distance in the process, pushing the monitor 6 to rotate 360 degrees along the circumferential direction of the root canal, and shooting a picture of the distribution condition of the crop root system in a 360-degree range along the root canal in the rotating process;
step a3, storing the shot pictures of the distribution status of the crop root system and the position of the monitor 6 relative to the root canal 2 during shooting, and splicing the pictures of the distribution status of the crop root system within 360 degrees along the root canal obtained by each monitor 6 into a complete picture of the distribution status of the crop root system at different depths according to the shooting sequence or the position sequence.
In order to provide sufficient root growth space for crops, the distance between every two rows of tubes among every two tubes can be set to be larger than the distance between every two rows of tubes. Supply crop root system to grow between each row of root canal, when sampling the crop root system, need splice the root system picture under the different degree of depth, consequently the interval that sets up root canal depth direction is less, avoids omitting the root system structure and not sampled.
The root canal can be a structure formed by splicing a plurality of transparent pipes into a straight pipeline. The head end and the tail end of each transparent pipe are respectively provided with a connecting part, the head ends or the tail ends of the adjacent transparent pipes are mutually inserted and fixed or are mutually screwed and fixed, the transparent pipe at the head end of each root canal 2 and the transparent pipe at the tail end are respectively provided with a sealing cover which is made of opaque materials and used for shielding light rays injected into the root canal 2 from the end parts.
In order to install the root canal, a perforating device shown in fig. 4 can be arranged in the root canal 4 for construction. The perforating device include: the device comprises a guide drill rod, a rotary table, a motor 23, a device protection shell, an L-shaped movable frame 73, an annular bracket 74, a small hydraulic support rod 22, a large hydraulic support rod 76, a hydraulic power unit, a limiting plate 71, a support plate 78, a damping column 72, a support base 75, a valve 79, a connecting device 791, a water tank and a laser range finder 77. The guide drill rod is hollow, the head of the guide drill rod is provided with a drainage groove, and the bottom of the guide drill rod is provided with a thread connected with a transparent root canal; the power source of the guide drill rod consists of a motor and a hydraulic power unit, an equipment protective shell is arranged outside the motor, a shell of the motor is arranged on the L-shaped movable frame and is connected with the front end of the annular bracket through small hydraulic support rods arranged on two sides of the shell, and the motor shell is pushed to horizontally move relative to the annular bracket under the control of the hydraulic power unit so as to finish the assembly and disassembly of the transparent root canal;
the annular bracket is carried on the L-shaped movable frame, the front end and the rear end are connected through a large hydraulic support rod, and the annular bracket is matched with a motor to realize a punching function under the control of a hydraulic power unit; the limiting plate and the supporting plate are connected with the supporting base through the damping columns, and in the implementation process, the limiting holes in the limiting plate are aligned with the reserved hole positions, so that the positioning is realized, and the vibration influence is reduced; the support base is connected with the stroke limiting hole of the L-shaped movable frame through the bolt, the stroke can be adjusted according to actual requirements, and the damping effect is achieved by installing damping columns on the two sides of the support base;
the water tank is connected with the rotary table through a connecting device, water is conveyed to the hollow pipe wall of the drill rod through a valve and a hose, and when the punching device works, the water flows out of a drainage groove in the top of the drill rod, so that the working resistance is reduced;
the laser range finder comprises a detection ring, a laser emitter and a control processor, wherein the detection ring is arranged in the center of a guide drill rod, and criss-cross scale marks and scale values corresponding to the scale marks are engraved on the upper surface of the detection ring so as to read the offset of a projection point of a light beam emitted by the laser emitter on the detection ring relative to a zero point of a coordinate; the laser transmitter is arranged at the rear end of the multi-channel punching device and connected with the control processor, and the laser positioning instrument is used for indicating a punching position; the multichannel perforating device finishes the assembly of the guide drill rod and the rotary table by controlling the hydraulic power unit to shrink the small hydraulic support rod, and the rotary advance of the drill bit is realized by the matching of the large hydraulic support rod and the motor to finish the perforating operation.
In the construction process:
firstly, fixing a perforating device in a root system detection channel 4 which is provided, and setting a guide drill rod of the perforating device to be aligned to the direction of installing a root canal in the root system detection channel 4 on the other side;
then, screwing and fixing the transparent tube at the rear end of a guide drill rod of the perforating device, and fixing the transparent tube on the perforating device, wherein a detection ring 25 and a water outlet 24 shown in fig. 5 are arranged at the position of a drill bit at the front end of the guide drill rod;
thirdly, supplying water to the drill bit through the water outlet 24, and simultaneously driving the guide drill rod to rotate to drill forward, wherein in the drilling process, a laser range finder arranged at the rear side of the drilling device aligns to a detection ring 25 arranged on the drill bit to correct the drilling direction;
fourthly, when one section of transparent pipe is drilled into the crop root growth area, detaching the rear end of the transparent pipe from the punching device, screwing and fixing the front end of the next section of transparent pipe at the rear end of the transparent pipe drilled into the crop root growth area, and screwing and fixing the rear end of the next section of transparent pipe on the punching device;
fifthly, repeating the third step and the fourth step until the first section of transparent pipe directly connected with the guide drill rod reaches the position for installing the root canal in the root system detection channel 4 at the other side, disassembling the guide drill rod, and installing a sealing cover at the end part of the root canal formed by connecting the transparent pipes in the crop root system growth area;
and sixthly, moving the perforating device to the installation position of the next pipe, and completing the installation of all the pipes according to the steps from the first step to the fifth step.
Therefore, the cylindrical transparent pipeline of the multi-section root canal is horizontally placed under a crop planting point, and the multi-section transparent pipe is connected into root system monitoring channels with different depths through the threads arranged at the front end and the rear end of the transparent pipe, so that the root system monitoring channels are uniformly distributed in the vertical direction to form a group of channels, and further are uniformly distributed in the horizontal direction to form a plurality of groups of channels;
the 360-degree multi-layer rotary image monitor used for sampling realizes sampling of root system distribution conditions of different depths, different angles and different positions of the underground through the column-type 360-degree rotary host, the LED light source, the motion module, the power supply and the data transmission and storage module. The host machine receives a remote control instruction through the data transmission and storage module and then controls the motion module to move in the channel, and the host machine can monitor the in-situ growth conditions of plant root systems at different depths in real time by matching with the LED light source and acquire a plurality of groups of crop root system phenotype data in real time, at regular time and at fixed points; by collecting crop root system images distributed near root canals of different depths, splicing a plurality of pictures at different time and space, and acquiring comprehensive information of the plant root system;
sealing covers are assembled at two ends of the root system monitoring pipeline, so that a light-shading environment is created, and the influence of external light on the root system is avoided;
the device for acquiring the root phenotype of the field crops, provided by the invention, can realize nondestructive, sustainable and high-frequency tracking observation and in-situ acquisition of soil root information through an endoscopic image acquisition technology, and avoids the defects that water distribution, nutrition distribution, soil structure and microorganism action in normal soil cannot be reflected by water culture or gel culture and the like. The technology can be used for carrying out nondestructive, high-flux and full-automatic root phenotype analysis on crop roots, and can be used for measuring analysis parameters such as root crown structures (including heel depth, crown width and the like), root crown areas, root lengths and the like.
The above are merely embodiments of the present invention, which are described in detail and with particularity, and therefore should not be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the spirit of the present invention, and these changes and modifications are within the scope of the present invention.
Claims (7)
1. A multi-channel acquisition system for field crop root phenotype, comprising:
the root system detection channel (4) comprises at least two root system detection channels which are embedded in parallel at the edge of a crop root system growth area, the depth of the root system detection channel (4) embedded underground at least reaches the growth depth range of a crop root system, the top of the root system detection channel (4) is provided with a lighting well (3), the lighting well (3) is made of transparent materials, and natural light is introduced into the root system detection channel (4) to provide illumination;
glass window (1), its setting is close to the lateral wall of crop root system in root system detection passageway (4), the regional marginal crop of crop root system growth, its root system is pressed close to glass window (1) is grown, still be provided with on glass window (1) and roll up curtain structure, roll up curtain structure and put down by glass window (1) top, shelter from the light that penetrates into in the crop root system growth region, perhaps upwards roll up when shooting crop root system distribution situation on glass window (1), shoot the natural light that well (3) introduced or for the lighting apparatus in root system detection passageway (4) for the root system shoots and provides the illumination;
the root canals (2) are arranged between the root system detection channels (4) which are parallel to each other, and comprise a plurality of groups which are parallel to each other and arranged in the crop root system growth area at intervals, the root canals (2) penetrate through the crop root system growth area, each group of root canals respectively comprise a plurality of roots which are horizontally arranged in the crop root system growth area at different depths, and the root canals are parallel to each other;
monitor (6), it sets up in root canal (2), along the root canal in the regional operation of the underground level of crop root system growth, monitor (6) are in along the rotation in a circumferential direction of root canal in root canal (2), shoot the distribution situation of each root canal 360 within range crop roots along the line.
2. The multichannel acquisition system for the phenotype of the root system of the field crops as claimed in claim 1, characterized in that the root canals (2) are arranged in an array of N rows and M columns, the distance between each row of the root canals is equal, the difference of the depths of the root canals embedded in the growth area of the root system of the crops is the same between each row of the root canals, the deepest depth of the root canals embedded in the root canals does not exceed the depth of the root system detection channel (4) embedded in the ground, and each root canal is respectively provided with a monitor (6).
3. The multi-channel acquisition system of a field crop root phenotype as claimed in claim 2 wherein the root canals (2) are arranged in an array of 5 rows and 5 columns, wherein the spacing between the root canals of each row is greater than the spacing between the root canals of each column.
4. The multi-channel obtaining system for the root phenotype of the field crops as claimed in claim 3, wherein each root canal (2) comprises a plurality of transparent tubes connected in a straight line, the head end and the tail end of each transparent tube are respectively provided with a connecting part, the connecting parts of the adjacent transparent tubes are mutually inserted and fixed or mutually screwed and fixed, the transparent tube at the head end and the transparent tube at the tail end of each root canal (2) are respectively provided with a sealing cover, and the sealing covers are made of opaque materials and can shield the light rays emitted into the root canal (2) from the end parts.
5. The multichannel acquisition system for the root phenotype of field crops as claimed in claim 4, characterized in that the main body of the monitor (6) is a cylindrical structure, and the monitor (6) is arranged inside the root canal (2) formed by connecting the transparent tubes;
one end or two ends of the monitor (6) are provided with motion modules (63) which comprise rotating wheels rotating along the circumferential direction of the cylindrical structure and driving wheels rotating along the axial direction of the cylindrical structure, the monitor (6) is driven by the driving wheels to move horizontally along the axial direction of the root canal in the root canal (2), and the rotating wheels are driven to rotate along the circumferential direction of the root canal;
the LED root-canal root-growth monitoring instrument is characterized in that an LED light source (61) is arranged in the middle of the monitoring instrument (6), and provides illumination required for shooting for an image acquisition unit in the monitoring instrument (6) when the monitoring instrument (6) shoots the distribution condition of crop roots within a 360-degree range along a root canal, and at least illuminates a shooting area of the image acquisition unit;
data department transmission storage module (62), it sets up in monitor (6), is connected with the image acquisition unit electricity, stores the photo of the distribution situation of the crop root system under the different angles that monitor (6) different positions were shot in the root canal.
6. A method for installing the multi-channel acquisition system of field crop root phenotype of any one of claims 1-5, characterized in that the steps comprise:
firstly, fixing a perforating device in a root system detection channel (4) which is arranged, and setting a guide drill rod of the perforating device to be aligned to the direction of installing a root canal in the root system detection channel (4) on the other side;
secondly, screwing and fixing the transparent pipe at the rear end of a guide drill rod of the perforating device, and fixing the transparent pipe on the perforating device, wherein a detection ring (25) and a water outlet (24) are arranged at the position of a drill bit at the front end of the guide drill rod;
thirdly, supplying water to the drill bit through a water outlet (24), and simultaneously driving the guide drill rod to rotate to drill forward, wherein in the drilling process, a laser range finder arranged at the rear side of the drilling device is aligned with a detection ring (25) arranged on the drill bit to correct the drilling direction;
fourthly, when one section of transparent pipe is drilled into the crop root growth area, detaching the rear end of the transparent pipe from the punching device, screwing and fixing the front end of the next section of transparent pipe at the rear end of the transparent pipe drilled into the crop root growth area, and screwing and fixing the rear end of the next section of transparent pipe on the punching device;
fifthly, repeating the third step and the fourth step until the first section of transparent pipe directly connected with the guide drill rod reaches the position for installing the root canal in the root system detection channel (4) at the other side, disassembling the guide drill rod, and installing a sealing cover at the end part of the root canal formed by connecting the transparent pipes in the crop root system growth area;
and sixthly, moving the perforating device to the installation position of the next pipe, and completing the installation of all the pipes according to the steps from the first step to the fifth step.
7. A method for multi-channel acquisition of root phenotype of field crops, characterized in that the following steps are executed for the multi-channel acquisition system of root phenotype of field crops as claimed in any one of claims 1 to 5:
a1, arranging a monitor (6) in each root canal (2) and recording the position of the monitor relative to the root canal (2);
step a2, turning on an LED light source (61) in a monitor (6), driving a driving wheel of the monitor (6) along the horizontal axial direction of the root canal, pushing the monitor (6) to walk along the root canal to the opposite side, driving a rotating wheel of the monitor (6) respectively every moving a shooting distance, pushing the monitor (6) to rotate 360 degrees along the circumferential direction of the root canal, and shooting a picture of the distribution condition of the crop root system within a range of 360 degrees along the root canal in the rotating process;
step a3, storing the shot pictures of the distribution status of the crop root system and the position of the monitor (6) relative to the root canal (2) during shooting respectively, and splicing the pictures of the distribution status of the crop root system within 360-degree range along the root canal obtained by each monitor (6) into a complete picture of the distribution status of the crop root system at different depths according to the shooting sequence or the position sequence.
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| CN202010077491.5A CN111239340B (en) | 2020-01-29 | 2020-01-29 | Multichannel acquisition system and installation method for field crop root phenotype |
| PCT/CN2020/102888 WO2021151280A1 (en) | 2020-01-29 | 2020-07-19 | Multichannel acquisition system for field crop root system phenotype, and installation method therefor |
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| CN202010077491.5A CN111239340B (en) | 2020-01-29 | 2020-01-29 | Multichannel acquisition system and installation method for field crop root phenotype |
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| CN111238395A (en) * | 2020-01-29 | 2020-06-05 | 南京慧瞳作物表型组学研究院有限公司 | 360-degree multi-level rotary image monitor for crop root system phenotype acquisition |
| CN111239340B (en) * | 2020-01-29 | 2021-08-03 | 南京慧瞳作物表型组学研究院有限公司 | Multichannel acquisition system and installation method for field crop root phenotype |
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