WO2019078646A2 - Composition contenant un extrait de culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation - Google Patents

Composition contenant un extrait de culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation Download PDF

Info

Publication number
WO2019078646A2
WO2019078646A2 PCT/KR2018/012351 KR2018012351W WO2019078646A2 WO 2019078646 A2 WO2019078646 A2 WO 2019078646A2 KR 2018012351 W KR2018012351 W KR 2018012351W WO 2019078646 A2 WO2019078646 A2 WO 2019078646A2
Authority
WO
WIPO (PCT)
Prior art keywords
composition
hair
soybean
less
extract
Prior art date
Application number
PCT/KR2018/012351
Other languages
English (en)
Korean (ko)
Other versions
WO2019078646A3 (fr
Inventor
이은정
강영규
박준성
신승현
나용주
Original Assignee
(주)아모레퍼시픽
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)아모레퍼시픽 filed Critical (주)아모레퍼시픽
Priority to SG11202003494XA priority Critical patent/SG11202003494XA/en
Priority to CN201880068260.9A priority patent/CN111246868A/zh
Publication of WO2019078646A2 publication Critical patent/WO2019078646A2/fr
Publication of WO2019078646A3 publication Critical patent/WO2019078646A3/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/366—Lactones having six-membered rings, e.g. delta-lactones
    • A61K31/37—Coumarins, e.g. psoralen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Definitions

  • the present invention relates to a composition for promoting hair loss prevention or hair growth comprising a soybean cultured tissue extract and a method for producing the same.
  • alopecia the most common type directly related to male hormone excess secretion is androgenic alopecia, commonly referred to as male pattern baldness.
  • male pattern baldness the most common type directly related to male hormone excess secretion.
  • androgenetic alopecia healthy hair becomes tapered and shortened, hair becomes weak and easily broken, which is called miniaturization.
  • miniaturization The hair follicles that undergo miniaturization eventually become thin and invisible and turn into short fur, resulting in hair loss.
  • the depilatory remedy such as propecia or minoxidil, which is commercially available, has little effect or has a large side effect, which limits its use depending on the subject, and may be burdensome for the consumer to use. In order to solve such a problem, it is inevitable to develop an anti-hair-loss agent or a hair growth promoter having excellent effects while using natural raw materials and the like.
  • the present invention provides a composition for preventing hair loss or for promoting hair growth, comprising an extract of a soybean culture tissue, wherein the soybean culture tissue is a soybean culturing muscle or a soybean callus.
  • the present invention in one aspect, can uniformly mass-produce a composition for preventing hair loss or hair growth promoting hair comprising a soybean cultured tissue of uniform quality, and the composition produced by such a method can prevent hair loss And hair growth promoting effect, it can be an alternative to overcome the disadvantages of existing hair loss preventing or hair growth promoting products.
  • FIG. 1 shows a culture of a soybean culturing muscle for 4 weeks using X-planting technique according to one aspect of the present invention.
  • FIG. 2 shows that the extract according to one aspect of the present invention enhances Wnt /? - catenin activity in a concentration-dependent manner.
  • FIG. 3 is a graph showing the effect of the extract according to one aspect of the present invention on the ratio of growth period to regressor period among follicular cycles.
  • explantation is also referred to as “explantation” or “in vitro culture”, and is mainly a technique for separating a part of plants and animals and culturing them in vitro. It mainly involves culturing over time for a certain period of time to survive in vitro and gradually show any developmental change.
  • General tissue culture tissue culture is also a method of in vitro culture.
  • In vitro culture according to one aspect of the present invention is also included in the in vitro culture.
  • the x-plantation technique includes raising at least one member selected from the group consisting of protoplasts, cells, tissues, organs, belly, seeds, cultivated muscles and parts of plants in an artificial incubator such as a medium supplemented with a specific component .
  • the present invention is a composition for preventing hair loss or promoting hair growth, which comprises an extract of soybean cultured tissue, wherein the soybean cultured tissue is soybean cultured muscle or soybean callus.
  • the present invention relates to an anti-hair loss or hair growth promoting method comprising administering an extract of a soybean culture tissue to an individual, wherein the soybean culture tissue is a soybean callus or soybean callus, Lt; / RTI >
  • the present invention relates to the use of an extract of a soybean culture tissue for use in the production of a composition for preventing hair loss or promoting hair growth, wherein the soybean culture tissue is an extract of soybean culture tissue Lt; / RTI >
  • the soybeans may be soybeans, Glycine gracilis , Sealite, Seomotei, Black tea, Cheongtaek, Cheongtae, Fence beans, Kidney beans, Stain Kidney beans, It can be flat beans.
  • the soybean varieties are not limited, but may be varieties for soy sauce, tofu, herb, rice or peanut in one aspect.
  • the rice varieties include Cheongjakong (breed protection application - 2001-38), Heugcheong (breeding protection application - 2000-27), Grami (breed protection application - 2000-25), Seonheukkong Application for varieties protection application -1999-20), black soybean and one kind black soybean (application for varieties protection application -1998-158).
  • the soybean is preferably a variety capable of germination and resistant to pests and diseases.
  • Such beans include, for example, myths, wishes, anpyeong, southeastern, colorful, alok, soho, calligraphy, tea ceremony, Poongsan herb, Iksan herb, Sobaek herb, Gwangan, monoplane and galaxy.
  • the soybean according to one aspect of the present invention is not limited to the soybean variety.
  • the flaked soybean is variously referred to as a flocked soybeans, a lobster soybeans, a hibiscus legume bean, a lead drage bean, and a flat soybeans.
  • the extract may be extracted with water, C1 to C6 lower alcohols, or a mixture thereof as a solvent.
  • the lower alcohol may be ethanol.
  • the alcohol concentration may be between 60 and 100% (w / v). In one aspect, the alcohol concentration is greater than 60% (w / v), greater than 70% (w / v), greater than 75% (w / v), greater than 80% ) Or more, 90% (w / v) or more, or 95% (w / v) or more. In another aspect, the alcohol concentration is less than 95% (w / v), less than 90% (w / v), less than 85% (w / v), less than 80% ) Or less, 75% (w / v) or less, 70% (w / v) or less, or 65% (w / v) or less.
  • the extract may comprise 5 to 15% by weight of cumestrol relative to the total weight of the extract.
  • the composition may further comprise 5 to 15% by weight of cumestrol relative to the total weight of the extract or composition.
  • the content of the quimestrol may be at least 5 wt%, at least 7 wt%, at least 8 wt%, at least 9 wt%, at least 10 wt%, at least 12 wt%, or at least 14 wt%.
  • the content of the quimestrol may be 15 wt% or less, 13 wt% or less, 12 wt% or less, 11 wt% or less, 10 wt% or less, 8 wt% or less, or 6 wt% or less.
  • the hair loss preventing or promoting hair growth promoting activity is at least one selected from the group consisting of proliferation of dermal papilla cells, increase in follicular stem cell activity, induction of hair follicle growth period, maintenance or extension of hair follicle growth period, .
  • composition according to one aspect of the present invention promotes hair follicle cell proliferation by increasing follicular stem cell activity, thereby preventing hair loss, promoting hair growth, alopecia areata (androgenetic alopecia, alopecia areata, alopecia areata, And alopecia due to microbial infection) can be improved and treated.
  • Hair is a process of regularly repeating the periodic changes of growth, regressor, and resting period, and each hair repeats independent generation and disappearance.
  • the mollusk cells are surrounded by the papillae, which are stromal cells, and have a circular shape.
  • the growth phase is the period of active cell division for the production of new hair.
  • the regressive period is when the growth of the hair follicles stops and rapidly shrinks, and the shape of the hair is similar to that of the club.
  • the resting period is the period when the hair cell activity is completely extinguished.
  • the hair is naturally pulled out by the growing hair that grows from the base of the resting hair, or by mechanical action such as combing or winding the hair .
  • alopecia refers to the fact that during this period, the hair rate of the growing period is shortened, and the number of abnormal hair removal is increased due to an increase in hair of the retrogressive or resting period. Since the composition according to one aspect of the present invention induces, maintains and prolongs the growth period of the hair follicle and inhibits the transition to the regenerator, the composition can be used to prevent hair loss, promote hair growth, promote various alopecia (including alopecia areata, Alopecia areata, alopecia due to drug, and alopecia due to microbial infection).
  • said hair loss prevention or hair growth promoting may be by increased beta-catenin transcription activity or increased Wnt / beta-catenin activity.
  • the composition according to one aspect of the present invention increases the transcription activity of ⁇ -catenin or increases Wnt / ⁇ -catenin activity in a concentration-dependent manner, whereby the follicle cycle is regulated (ie, (Alopecia areata, alopecia areata, alopecia due to a drug, and alopecia due to microbial infection), and the like can be utilized for the prevention of hair loss, promotion of hair growth, and various alopecia It is.
  • the route of administration of the composition is not limited, but may be preferably transdermal or dermatological.
  • the concentration of the extract in the composition may be between 10 and 100 ppm (w / v).
  • the dosage level is greater than or equal to 10 ppm (w / v), greater than 20 ppm (w / v), greater than 25 ppm (w / v), greater than 30 ppm (w / v), at least 45 ppm (w / v), at least 50 ppm (w / v), at least 60 ppm v) or higher, or 95 ppm (w / v) or higher.
  • the dosage level is not more than 100 ppm (w / v), not more than 90 ppm (w / v), not more than 80 ppm (w / v), not more than 70 ppm (w / v), no more than 45 ppm (w / v), no more than 40 ppm (w / v), no more than 35 ppm (w / v), no more than 30 ppm v) or less, or 15 ppm (w / v) or less.
  • the dose of the extract by administration of the composition may be from 5 mg / kg / day to 300 mg / kg / day.
  • the dose is at least 5 mg / kg / day, at least 10 mg / kg / day, at least 30 mg / kg / day, at least 50 mg / kg / day, at least 80 mg / kg / day, kg / day, greater than 140 mg / kg / day, greater than 160 mg / kg / day, greater than 180 mg / kg / day, greater than 200 mg / kg / day, greater than 250 mg / kg / day, or greater than 280 mg / .
  • the dosage is less than or equal to 300 mg / kg / day, less than or equal to 280 mg / kg / day, less than or equal to 250 mg / kg / day, less than or equal to 200 mg / kg / day, less than or equal to 180 mg / no more than 10 mg / kg / day, no more than 120 mg / kg / day, no more than 100 mg / kg / day, no more than 80 mg / kg / day, no more than 50 mg / / kg / day.
  • the composition may be a food, pharmaceutical, or cosmetic composition.
  • the composition may be a pharmaceutical composition for improving or treating one or more selected from the group consisting of androgenic alopecia, alopecia areata, alopecia areata, alopecia due to a drug, and alopecia due to microbial infection.
  • the food composition according to one aspect of the present invention may be a health food composition.
  • determination of the dose of the compound is within the level of those skilled in the art, and may be varied depending on the age, health condition, It depends on factors.
  • the formulation of the food composition is not particularly limited and may be formulated into, for example, tablets, granules, pills, powders, liquid preparations such as drinks, caramels, gels, bars, tea bags and the like.
  • the food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients.
  • composition may be administered by various methods such as simple ingestion, drinking, injection administration, spray administration or squeeze administration.
  • the health food composition according to one aspect of the present invention can be a health functional food and can be used not only in various foods such as chewing gum, caramel product, candy, ice cream, confectionery and bread, soft drinks, mineral water, Etc., and may be a functional food including vitamins, minerals, and the like.
  • the health food composition according to one aspect of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate etc.) A salt thereof, alginic acid and its salt, an organic acid, a protective colloid thickener, a pH adjuster, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink or the like.
  • the health food compositions according to one aspect of the present invention may comprise natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally comprised in the range of 0 to about 50 parts by weight per 100 parts by weight of the composition according to one aspect of the present invention.
  • the cosmetic composition according to one aspect of the present invention is a composition for skin, nail and / or hair, for example, as a composition for softening longevity, convergent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, eye essence, essence, Body lotion, body cream, body essence, body cleanser, hair dye, shampoo, rinse, correcting agent, hair tonic, ointment, gel, cream, patch, spray, powder and skin An adhesive type, and the like, but the present invention is not limited thereto.
  • ingredients other than the above-mentioned essential ingredients can be suitably selected and blended by those skilled in the art depending on the kind of the external preparation, the purpose of use, and the like.
  • the cosmetic composition may be provided in all formulations suitable for topical application.
  • it may be provided as a formulation of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a micro needle, a foam or an aerosol composition have.
  • Compositions of such formulations may be prepared according to conventional methods in the art.
  • the cosmetic composition according to the present invention may further contain the components included in the functional additive and the general cosmetic composition in addition to the compounds, extracts or fractions of the present specification.
  • the functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.
  • the cosmetic composition according to the present disclosure may contain other ingredients, which may preferably have a synergistic effect on the main effect, without impairing the main effect.
  • the cosmetic composition according to the present invention may further include a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a perfume, a cold agent or a limiting agent.
  • a moisturizing agent an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a perfume, a cold agent or a limiting agent.
  • the composition may be an external preparation for skin.
  • the composition for external application for skin may be a composition such as a cosmetic preparation, an oral preparation, a cleaning agent, a pharmacy, and a quasi-drug, but is not limited thereto.
  • the external preparation for skin is not particularly limited in its formulation.
  • composition for external application for skin of the present invention may further comprise at least one selected from a salt for a functional purpose and a pH adjusting agent for pH adjustment.
  • the salt may be selected from inorganic salts, organic salts and / or organic-inorganic salts for ion shielding, moisturizing, ultraviolet shielding and the like.
  • the salt may be selected from sodium chloride (NaCl), sodium phosphate (Na3PO4) and calcium chloride (CaCl2).
  • the pH adjusting agent is selected from an acid or base and may be selected from the group consisting of hydrochloric acid, sulfuric acid, tartaric acid, citric acid, phosphoric acid, acetic acid, lactic acid solution, sodium lactate, sodium hydroxide, potassium hydroxide, Oleamine, ammonia, and the like.
  • the composition for external application for skin of the present invention may be a cosmetic, a pharmaceutical or a quasi-drug composition.
  • the cosmetic, pharmacological or quasi-drug composition may contain preservatives, stabilizers, wettable or emulsifying accelerators, adjuvants such as salts for controlling osmotic pressure and / May additionally contain useful substances.
  • the composition may be formulated into lotions, creams, ointments or gels.
  • the skin external composition composition is preferably transdermally administered.
  • the dosage of the active ingredient of the pharmaceutical or quasi-drug composition will depend on the age, sex, and weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art.
  • compositions according to one aspect of the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.
  • Formulations for oral administration may be in the form of tablets, pills, soft and hard capsules, granules, powders, granules, solutions, emulsions or pellets, It is not.
  • Formulations for parenteral administration may be, but are not limited to, solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches or spray formers.
  • the formulations may be readily prepared according to conventional methods in the art and may be prepared by conventional means including, but not limited to, surfactants, excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, colorants, spices, stabilizers, preservatives, Other adjuvants may also be included.
  • the amount or dosage of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, the administration route or the judgment of the prescriber. Determination of the amount of application based on these factors is within the level of those skilled in the art.
  • a process for producing a bioreactor comprising the steps of: (1) placing soybean cultivating muscle in a medium in a bioreactor, (2) drying the cultured muscle through the step (1); (3) dipping the resultant obtained in the step (2) in an alcohol and extracting it; And (4) the step (3), followed by filtration and drying, to thereby uniformly mass-produce a composition for preventing hair loss or promoting hair growth.
  • a process for producing a bioreactor comprising the steps of: (1) placing soybean cultivating muscles in a medium in a bioreactor, while maintaining constant air supply in the bioreactor; (2) drying the cultured muscle through the step (1); (3) dipping the resultant obtained in the step (2) in an alcohol and extracting it; And (4) the step (3), followed by filtration and drying, so as to uniformly and massively produce the extract of the soybean cultured tissue.
  • the method may comprise germinating the soybeans in the medium prior to step (1). In another aspect, the method may also include the step of deriving the soy cultured muscle from the soybean plant prior to step (1).
  • soybean extracts grown in a non-cultured state and extracted by a common method are not uniform in composition, so it is very difficult to uniformly mass-produce a composition for achieving a specific purpose.
  • the bioreactor may be a Stirred Tank Reactor, a Bubble Cloumn Reactor, an Air Lift Reactor, a Fludized Bed Reactor, a Fixed Bed Reactor, / Fixed / Packed Bed Reactor, or Tower Fermenter, preferably a Bulb Type bubble bioreactor.
  • the medium of step (1) may be indole-3-butyric acid (IBA), carbon source added.
  • the carbon source is selected from the group consisting of glucose, fructose, mannose, ribose, arabinose, xylose, galactose, sucrose, cellobiose, trehalose, lactose, raffinose, amylose, starch, sorbitol, mannitol, and glycerol It may be more than one selected.
  • the medium of step (1) may be Murashige and Skoog medium.
  • the pH of the medium when the soybean cultivating muscle is placed in the medium, the pH of the medium may be 4.8 to 6.8.
  • the pH may be at least 4.8, at least 5, at least 5.2, at least 5.4, at least 5.6, at least 5.8, at least 6.0, at least 6.2, at least 6.4, or at least 6.6.
  • the pH may be less than 6.8, less than 6.6, less than 6.4, less than 6.2, less than 6.0, less than 5.8, less than 5.6, less than 5.4, less than 5.2, less than 5.0.
  • the density of the bean culture roots in the medium may be between 2 and 6 g / L. In one aspect, the density may be greater than or equal to 2 g / L, greater than or equal to 3 g / L, greater than or equal to 4 g / L, or greater than or equal to 5 g / L. In another aspect, the density may be 6 g / L or less, 5 g / L or less, 4 g / L or less, or 3 g / L or less.
  • step (1) may be carried out under a dark condition.
  • the step (1) may be carried out at 19 to 25 ° C.
  • the temperature may be at least 19 ° C, at least 20 ° C, at least 21 ° C, at least 22 ° C, at least 23 ° C, or at least 24 ° C.
  • the temperature may be less than or equal to 25 ° C, less than or equal to 24 ° C, less than or equal to 23 ° C, less than or equal to 22 ° C, less than or equal to 21 ° C, or less than or equal to 20 ° C.
  • the air supply amount in the step (1) may be 0.05 to 0.4 vvm (air volume / culture volume per min).
  • the air supply is at least 0.05 vvm, at least 0.08 vvm, at least 0.1 vvm, at least 0.12 vvm, at least 0.14 vvm, at least 0.16 vvm, at least 0.18 vvm, at least 0.2 vvm, at least 0.22 vvm, at least 0.24 vvm, at least 0.26 vvm Or more, 0.28 vvm or more, 0.3 vvm or more, 0.32 vvm or more, 0.34 vvm or more, 0.36 vvm or more, or 0.38 vvm or more.
  • the air supply amount is less than or equal to 0.4 vvm, less than 0.38 vvm, less than 0.36 vvm, less than 0.34 vvm, less than 0.32 vvm, less than 0.3 vvm, less than 0.28 vvm, less than 0.26 vvm, less than 0.24 vvm, less than 0.22 vvm 0.18 vvm or less, 0.16 vvm or less, 0.14 vvm or less, 0.12 vvm or less, 0.1 vvm or less, 0.08 vvm or less, or 0.06 vvm or less.
  • the step (1) may be carried out for 2 to 5 weeks. In one aspect, the step (1) may be carried out for more than two weeks, three weeks or four weeks or more. In another aspect, the proliferation of step (1) may be performed for 5 weeks or less, 4 weeks or 3 weeks or less.
  • the alcohol concentration in step (3) may be 60 to 100% (w / v). In one aspect, the alcohol concentration is greater than 60% (w / v), greater than 70% (w / v), greater than 80% (w / v), greater than 90% (w / v) ) Or more. In another aspect, the alcohol concentration is less than or equal to 100% (w / v), less than 95% (w / v), less than 90% (w / v), less than 85% (w / Or less, 75% (w / v) or less, 70% (w / v) or less, and 65% (w / v) or less.
  • the weight ratio of the dried product to the alcohol is 1:50 or less, 1:45 or less, 1:40 or less, 1:35 or less, 1:32 or less, 1:30 or less, 1:25 or less, or 1: 22 or less. In another aspect, the weight ratio is 1:20 or more, 1:22 or more, 1:24 or more, 1:26 or more, 1:28 or more, 1:31 or more, 1:33 or more, 1:35 or more, 1:40 Or more, 1:45 or more, or 1:48 or more.
  • the extraction time of step (3) may be 20 to 28 hours. In one aspect, the extraction time may be at least 20 hours, at least 22 hours, at least 24 hours, at least 25 hours, or at least 26 hours. In another aspect, the extraction time may be less than or equal to 28 hours, less than or equal to 26 hours, less than or equal to 25 hours, less than or equal to 24 hours, less than or equal to 22 hours, or less than or equal to 21 hours.
  • Soybean ( Glycine max. ) And flat soybeans ( Glycine gracilis ) were germinated in the medium in the MS medium (Murashige and Skoog medium, Duchefa, Haarlem, The Netherlands). (In particular, derived from the root of soybeans) by using the root, dorsiflexion, cotyledon, etc. of the in-flight bean plant.
  • a bulb type bioreactor having an air volume of 3L (generally available in the market, (Murashige and Skoog medium, Duchefa, Haarlem, Netherlands) supplemented with 4 mg / L of indole-3-butyric acid (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and 30 g / ), And soybean cultured muscle was subcultured and propagated at 3-week intervals with fresh medium. The medium was adjusted to pH 5.8 with 1N NaOH and sterilized at 121 ⁇ and 1.2 atm for 35 minutes. The cultured muscle was proliferated for 3 weeks.
  • the cultured muscle was cut to 1-1.5 cm and inoculated on the medium at a density of 4 g / L based on the weight of the living body, and cultured under the dark condition maintained at 22 ⁇ 1 ° C. In this process, pesticides and fertilizers were not used at all.
  • the air supply was constantly adjusted to 0.1 vvm (air volume / culture volume per min) using air flow meters (RMA series; Dwyer Instruments, Inc., USA) In order to maintain a constant temperature, the supplied air is passed through an air condenser capable of condensing compressed air, a filter capable of removing impurities and an air drier in sequence, and then introduced into the bioreactor Respectively.
  • soybean cultured root extract of uniform quality could be produced throughout the year, unlike ordinary soybean (seed or plant derived) .
  • the soybean cultured muscle propagated for 4 weeks using the bioreactor of Example 1 was subjected to hot air drying and then immersed in 80% (w / v) ethanol so that the weight ratio of ethanol to ethanol was 1:30, and extracted at room temperature for 24 hours.
  • the extract was filtered using a filter paper, and then the solvent was evaporated to dryness to obtain a powder.
  • the extract was found to contain various active materials, including about 10% by weight of cumestrol.
  • NIH3T3 (WNT) cells were seeded in 96 well plates at a density of 10,000 cells / well and cultured for 24 hours (5% CO 2 , 37 ° C). After that, negative control cells were not treated and positive control cells LiCl (lithium chloride), and the extracts were treated for 24 hours in the experimental group.
  • ONE-Glo reagent 100 ⁇ l was added to each well of a 96-well plate, and reacted for 3 minutes. Luciferase activity was measured with a luminometer (Victor luminometer).
  • the transcriptional activity of ⁇ -catenin was increased as the concentration of the extract increased (see FIG. 2).
  • Wnt / ⁇ -catenin activity was 3.28 times higher than that of the control group when 50 ppm (w / w) of the extract was treated.
  • SARE means the above-mentioned soybean adventitious root extract. Maintenance and activation of Wnt signaling through the beta-catenin pathway is necessary for the hair-inducing activity of the dermal papilla and is also necessary for anagen-phase maintenance in the dermal papilla cells. The Wnt /?
  • the extract of the present invention is effective for induction of hair, promotion of hair growth, Respectively.
  • Fifteen follicular samples per group were collected from six groups of human beings and 90 samples were separated.
  • the isolated hair follicles were placed in a 24-well plate (Nunc, Wiesbaden, Germany) at a rate of 500 ⁇ l, followed by 5 to 6 samples per well.
  • the extracts were treated with follicular samples in the medium at different concentrations (0, 10, and 50 ppm), and the medium was changed every 2 to 3 days.
  • the follicles were photographed using a microscope and the cycles were classified according to morphological criteria.
  • the ratio of the catagen phase to the anagen phase was measured from the hair follicle cycle (HF cycle).
  • HF cycle hair follicle cycle
  • keratinocyte apoptosis is increased and keratinocyte proliferation is suppressed in the transition from growth phase to retrograde phase.
  • the ratio of the growth period to the retrograde period was 2 times higher than that of the control group (FIG. 3). That is, when the extract of the present invention was used, a remarkable extension of the growing period occurred in the HF cycle.
  • MNX is minoxidil
  • SARE soybean adventitious root extract in FIG. 3
  • a soft capsule was prepared by mixing 10 mg of the extract of Example 2, 80 to 140 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of vegetable hardening oil, 4 mg of yellow radish and 6 mg of lecithin and filling them into 1 capsule according to a conventional method.
  • composition ratio of the vitamin and the mineral mixture is relatively mixed with the ingredient suitable for health food
  • compounding ratio of the vitamin and the mineral mixture may be arbitrarily varied.
  • the ingredients may be mixed according to a conventional method for producing health food, Can be used for the production of health food compositions.
  • Example 2 0.2% by weight of the extract of Example 2, 2.00% by weight of polyethylene glycol monostearate, 5.00% by weight of self emulsifying monostearate glycerin, 4.00% by weight of propylene glycol, 6.00% by weight of squalane, 6.00% by weight of trihexylhexane glyceryl , 1.00% by weight of sphingoglycolipids, 7.00% by weight of 1,3-butylene glycol, 5.00% by weight of beeswax, and purified water.
  • the ointment was also prepared by controlling the amount of purified water and adjusting the viscosity.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne une composition contenant un extrait d'une culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation. Selon un aspect de la présente invention, l'utilisation de ce procédé conduit à la production uniforme et massive d'une composition contenant une culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux, et la composition préparée par ledit procédé présente d'excellents effets pour prévenir la perte de cheveux et favoriser la pousse des cheveux et n'a pas d'effets secondaires contrairement à un agent de traitement contre la perte des cheveux existant.
PCT/KR2018/012351 2017-10-20 2018-10-18 Composition contenant un extrait de culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation WO2019078646A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SG11202003494XA SG11202003494XA (en) 2017-10-20 2018-10-18 Composition, containing extract from bean culture, for prevention of hair loss or promotion of hair growth and method for preparing same
CN201880068260.9A CN111246868A (zh) 2017-10-20 2018-10-18 包含豆培养组织提取物的用于防脱发或者促进生发的组合物及其制备方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0136235 2017-10-20
KR1020170136235A KR102249710B1 (ko) 2017-10-20 2017-10-20 콩 배양조직 추출물을 포함하는 탈모방지 또는 육모촉진용 조성물 및 이의 제조방법

Publications (2)

Publication Number Publication Date
WO2019078646A2 true WO2019078646A2 (fr) 2019-04-25
WO2019078646A3 WO2019078646A3 (fr) 2019-06-06

Family

ID=66174162

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/012351 WO2019078646A2 (fr) 2017-10-20 2018-10-18 Composition contenant un extrait de culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation

Country Status (4)

Country Link
KR (1) KR102249710B1 (fr)
CN (1) CN111246868A (fr)
SG (1) SG11202003494XA (fr)
WO (1) WO2019078646A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210045684A (ko) 2019-10-17 2021-04-27 (주)아모레퍼시픽 이소바바찰콘을 포함하는 탈모방지용 조성물

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100348155B1 (ko) 2000-04-28 2002-08-09 김상묵 자연산 상황버섯 추출물을 함유하는 발모제 조성물 및이의 제조방법
CN101318947A (zh) * 2008-07-10 2008-12-10 上海交通大学 利用组织培养生产大豆异黄酮的方法
KR101063574B1 (ko) * 2008-07-22 2011-09-07 (주)아모레퍼시픽 검은콩을 함유한 모발용 화장료 조성물
CN101721634B (zh) * 2009-12-15 2013-10-02 王洪亮 黑五谷萃取液
WO2011122872A2 (fr) * 2010-03-31 2011-10-06 (주)아모레퍼시픽 Composition comprenant du coumestrol ou un extrait de fèves contenant du coumestrol
CN104771395A (zh) * 2010-03-31 2015-07-15 株式会社爱茉莉太平洋 含有香豆雌酚或包含香豆雌酚的豆提取物的组合物
KR20120121303A (ko) * 2011-04-26 2012-11-05 노효만 안토시아닌 워터를 함유하는 탈모방지용 두발 화장료 조성물
KR20120136583A (ko) * 2011-06-09 2012-12-20 (주)아모레퍼시픽 탈모 방지 또는 육모 촉진용 조성물
KR20120136581A (ko) * 2011-06-09 2012-12-20 (주)아모레퍼시픽 콩 추출물을 포함하는 탈모 방지 또는 육모 촉진용 조성물
KR101315969B1 (ko) * 2011-06-30 2013-10-08 주식회사 이롬 찔레 추출물을 유효성분으로 포함하는 탈모 방지 및 발모 촉진용 조성물
CN102697681A (zh) * 2012-05-28 2012-10-03 龚盛昭 一种具有抑制5α-还原酶活性的中药组合物及其在防脱发化妆品中的应用
KR101957845B1 (ko) * 2012-07-31 2019-03-14 (주)아모레퍼시픽 콩뿌리 추출물을 포함하는 모발 및 두피 상태 개선용 조성물
KR101492141B1 (ko) * 2012-11-09 2015-02-11 주식회사 바이오에프디엔씨 식물 세포 내 생리활성물질 함량 증가를 위한 고주파 장치 및 이를 이용한 식물 세포 배양방법
KR101516070B1 (ko) * 2013-06-27 2015-05-04 심상원 두피용 화장품과 그 제조 방법
CN104041416B (zh) * 2014-06-27 2015-08-26 聊城大学 一种利用红豆杉叶片愈伤组织产生紫杉醇的方法
WO2016171464A1 (fr) * 2015-04-20 2016-10-27 (주)이지희컴퍼니 Composition contenant de l'eau de thé vert pour le cuir chevelu et les cheveux
CN105168046B (zh) * 2015-09-08 2018-01-30 珀莱雅化妆品股份有限公司 一种含植物提取物的生发组合物
KR101841603B1 (ko) * 2016-03-30 2018-05-14 안동석창포영농조합법인 석창포 추출물을 함유하는 발모촉진과 탈모방지 및 비듬방지를 위한 샴푸 조성물과 조성물의 제조방법 및 조성물을 이용하여 제조된 샴푸

Also Published As

Publication number Publication date
CN111246868A (zh) 2020-06-05
KR102249710B1 (ko) 2021-05-10
KR20190044175A (ko) 2019-04-30
SG11202003494XA (en) 2020-05-28
WO2019078646A3 (fr) 2019-06-06

Similar Documents

Publication Publication Date Title
KR20170055771A (ko) 식물 추출물을 포함하는 탈모 방지 및 발모 개선용 조성물
KR101828584B1 (ko) 참여로, 가래나무, 도깨비 부채 및 모과나무의 복합추출물을 포함하는 항산화 또는 미백용 조성물
KR101762796B1 (ko) 복합생약추출물의 발효물을 포함하는 탈모방지용 조성물
KR20150007464A (ko) 두류 태좌 세포 배양 추출물을 함유한 항노화, 항염, 항산화 화장료 조성물
KR20160109764A (ko) 구기자 캘러스 추출물을 유효성분으로 포함하는 피부상태 개선용 조성물
KR20120052894A (ko) 한련초 추출물, 이의 분획물, 터트티에닐 유도체 또는 이의 약학적으로 허용가능한 염을 포함하는 탈모방지 및 발모촉진용 조성물
KR101965595B1 (ko) 탈모 방지 또는 육모 촉진용 조성물
WO2016056781A1 (fr) Composition pour prévenir la chute des cheveux ou stimuler la pousse des cheveux comprenant un extrait d'artemisia umbelliformis
KR102323101B1 (ko) 산수국 추출물을 포함하는 모발과 두피 상태 개선용 조성물
KR102635306B1 (ko) 단풍콩잎 추출물을 함유하는 탈모방지 또는 발모개선용 조성물
WO2019078646A2 (fr) Composition contenant un extrait de culture de haricot pour prévenir la perte de cheveux ou favoriser la pousse des cheveux et son procédé de préparation
KR101824497B1 (ko) 피토에스트로겐을 유효성분으로 포함하는 탈모방지 및 모발 성장 촉진용 조성물
KR101229762B1 (ko) 육모촉진 및 탈모예방 개선용 외용제조성물
WO2020138834A1 (fr) Composition pour la prévention ou le traitement de maladies de la peau comprenant de la spirée couronne de mariée
KR101965594B1 (ko) 콩 추출물을 포함하는 탈모 방지 또는 육모 촉진용 조성물
WO2016056780A1 (fr) Composition pour prévenir la chute des cheveux ou stimuler la pousse des cheveux comprenant un extrait de scutellaria alpina
KR20140004463A (ko) 새싹추출물 및 새순추출물을 이용한 피부 외용제 조성물
KR102353372B1 (ko) 수벌번데기 추출물을 포함하는 탈모방지 또는 육모촉진용 조성물
KR20050102571A (ko) 산삼세포의 제조방법
WO2011126189A1 (fr) Composition de prévention de la chute des cheveux et de stimulation de la croissance des cheveux
WO2021215882A1 (fr) Composition pour prévenir la chute des cheveux ou favoriser la pousse des cheveux comprenant un extrait de péricarpe de camélia en tant que principe actif
WO2020111297A1 (fr) Composition cosmétique comprenant comme principe actif un extrait composite à base de thym pour hydrater la peau
WO2023055221A1 (fr) Composition comprenant un extrait d'avoine pour atténuer la chute des cheveux et favoriser la pousse des cheveux
WO2020055171A1 (fr) Composition cosmétique permettant d'améliorer des rides et d'augmenter l'élasticité comprenant un extrait de fermentation de phragmites communis
KR100701272B1 (ko) 백발억제 및 흑모유발 촉진용 조성물

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18868337

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18868337

Country of ref document: EP

Kind code of ref document: A2