WO2018031404A1 - Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity - Google Patents

Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity Download PDF

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Publication number
WO2018031404A1
WO2018031404A1 PCT/US2017/045496 US2017045496W WO2018031404A1 WO 2018031404 A1 WO2018031404 A1 WO 2018031404A1 US 2017045496 W US2017045496 W US 2017045496W WO 2018031404 A1 WO2018031404 A1 WO 2018031404A1
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cells
mesoderm
kdr
mir
aplnr
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English (en)
French (fr)
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Mervin C. Yoder
Nutan PRASAIN
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Indiana University Research and Technology Corp
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Indiana University Research and Technology Corp
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Priority to CN201780048596.4A priority Critical patent/CN109689858B/zh
Priority to US16/323,722 priority patent/US11739293B2/en
Priority to JP2019507218A priority patent/JP7045363B2/ja
Priority to BR112019002584-7A priority patent/BR112019002584A2/pt
Priority to CA3033381A priority patent/CA3033381A1/en
Priority to AU2017308738A priority patent/AU2017308738B2/en
Priority to EP17840057.8A priority patent/EP3497204B1/en
Priority to KR1020197006515A priority patent/KR102456031B1/ko
Application filed by Indiana University Research and Technology Corp filed Critical Indiana University Research and Technology Corp
Priority to IL264273A priority patent/IL264273B2/en
Publication of WO2018031404A1 publication Critical patent/WO2018031404A1/en
Anticipated expiration legal-status Critical
Priority to JP2022044157A priority patent/JP2022081657A/ja
Priority to US18/159,725 priority patent/US20230174929A1/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/165—Vascular endothelial growth factor [VEGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Definitions

  • the present disclosure relates to the fields of cell and tissue biology. More particularly, the present disclosure relates to lineage-specific differentiation of pluripotent stem cells into mesoderm cells and/or endothelial colony forming cell-like cells (ECFC-like cells) that can form blood vessels in vivo.
  • ECFC-like cells endothelial colony forming cell-like cells
  • FIG. 1A depicts a schematic of a one-step, 2D, serum and feeder-free mesoderm lineage differentiation protocol.
  • FIGS. 5A-5C illustrate that D4 SSEA5-depleted KNA + mesoderm cells display transcripts typically enriched in lateral plate/extra-embryonic mesoderm cells, and exhibit enhanced formation of NRP-1 + CD31 + cells with ECFC competence upon in vitro ECFC differentiation.
  • FIG. 6F depicts a schematic showing a mesoderm lineage differentiation protocol in the presence of Fc-NRP-1 dimer.
  • FIG. 6G depicts a bar graph showing that dimeric Fc-NRP-1 treatment of
  • FIG. 7A depicts fold change in expression of various miRNAs in Day 4 SSEA5 " KNA + mesoderm cells relative to hiPSCs (indicated by 0 on Y-axis).
  • FIG. 7B depicts a schematic showing a mesoderm lineage differentiation protocol that involves contacting the cells undergoing mesoderm induction with specific miRNA mimics or inhibitors or both mimics and inhibitors (3m3i) or mimic/inhibitor controls.
  • FIG. 7C depicts a bar chart showing frequency of Day 4 SSEA5 " KNA + mesoderm cells obtained using the protocols of FIG. 7B.
  • endothelial differentiation medium refers to any nutrient medium that supports and/or enhances differentiation of pluripotent cells into cells of the endothelial lineage.
  • kits can include various reagents for use with the present disclosure in suitable containers and packaging materials.
  • the kit can include one or more controls for use with the present disclosure in suitable containers and packaging materials.
  • In vivo implantation/vessel formation assay (including functional assay): Pig skin type I collagen was used to generate three-dimensional (3D) cellularized collagen matrices as previously described (Critser et al., 2010). Briefly, type 1 collagen gel mixture was prepared by mixing together ice-cold porcine skin collagen solution, 10% v/v human platelet lysate in 0.01 N HCL, and neutralized with phosphate buffered saline and 0.1 N NaOH to achieve neutral pH (7.4). Neutralized gel mixtures (-1.5 mg/mL) were kept on ice before induction of polymerization by warming at 37°C, in 5% C0 2 .

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PCT/US2017/045496 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity Ceased WO2018031404A1 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
EP17840057.8A EP3497204B1 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
JP2019507218A JP7045363B2 (ja) 2016-08-10 2017-08-04 インビボで血管形成能を有する中胚葉細胞および／または血管内皮コロニー形成細胞様細胞を作製する方法
BR112019002584-7A BR112019002584A2 (pt) 2016-08-10 2017-08-04 método para a geração de células do tipo células formadoras de colônia do mesoderma e/ou endoteliais apresentando capacidade de formação de vasos sanguíneos in vivo
CA3033381A CA3033381A1 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
AU2017308738A AU2017308738B2 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
CN201780048596.4A CN109689858B (zh) 2016-08-10 2017-08-04 用于产生具有体内血管形成能力的中胚层和/或内皮集落形成细胞样细胞的方法
US16/323,722 US11739293B2 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
KR1020197006515A KR102456031B1 (ko) 2016-08-10 2017-08-04 생체 내 혈관 형성 능력을 갖는 중배엽 및/또는 내피 콜로니 형성 세포-유사 세포를 생성시키기 위한 방법
IL264273A IL264273B2 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
JP2022044157A JP2022081657A (ja) 2016-08-10 2022-03-18 インビボで血管形成能を有する中胚葉細胞および／または血管内皮コロニー形成細胞様細胞を作製する方法
US18/159,725 US20230174929A1 (en) 2016-08-10 2023-01-26 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity

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US201662372907P 2016-08-10 2016-08-10
US62/372,907 2016-08-10

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US16/323,722 A-371-Of-International US11739293B2 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
US18/159,725 Division US20230174929A1 (en) 2016-08-10 2023-01-26 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity

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US (2) US11739293B2 (enExample)
EP (1) EP3497204B1 (enExample)
JP (2) JP7045363B2 (enExample)
KR (1) KR102456031B1 (enExample)
CN (1) CN109689858B (enExample)
AU (1) AU2017308738B2 (enExample)
BR (1) BR112019002584A2 (enExample)
CA (1) CA3033381A1 (enExample)
IL (1) IL264273B2 (enExample)
WO (1) WO2018031404A1 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2021025083A1 (enExample) * 2019-08-05 2021-02-11
WO2021176233A1 (en) * 2020-03-06 2021-09-10 Imperial College Innovations Limited Method of generating endothelial cells
EP3755354A4 (en) * 2018-02-21 2022-01-19 Indiana University Research and Technology Corporation COMPOSITIONS AND METHODS FOR THE TREATMENT OR PROPHYLAXIS OF A PERFUSION DISORDER
WO2022040798A1 (en) * 2020-08-25 2022-03-03 University Health Network Compositions and methods for generating human yolk sac-like hematopoietic cells
CN115137740A (zh) * 2022-03-29 2022-10-04 广州大学 miRNA497b与miRNA-5106在制备治疗缺血心肌的药物中的应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022002418A (es) * 2019-08-28 2022-03-22 Astellas Inst For Regenerative Medicine Composiciones y metodos de tratamiento de enfermedades vasculares.
CN116769710A (zh) * 2023-01-28 2023-09-19 深圳市寰宇生物科技有限公司 一种从人诱导性多能干细胞分化的巨噬细胞及其制备方法和应用
CN117070442B (zh) * 2023-10-18 2024-01-30 北京大学 静脉内皮细胞及其制备方法、静脉血管畸形模型及其制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010011352A2 (en) * 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
US20100261274A1 (en) * 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
WO2015138634A1 (en) * 2014-03-11 2015-09-17 Indiana University Research And Technology Corporation Method for generating endothelial colony forming cell-like cells
US20150329821A1 (en) * 2012-10-19 2015-11-19 Agency For Science, Technology And Research Methods of differentiating stem cells into one or more cell lineages

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017390B2 (en) 2007-07-20 2011-09-13 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
US20130058905A1 (en) 2011-08-23 2013-03-07 Igor Slukvin Angiohematopoietic Progenitor Cells
CA2906188A1 (en) * 2013-03-15 2014-09-18 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100261274A1 (en) * 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
WO2010011352A2 (en) * 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
US20150329821A1 (en) * 2012-10-19 2015-11-19 Agency For Science, Technology And Research Methods of differentiating stem cells into one or more cell lineages
WO2015138634A1 (en) * 2014-03-11 2015-09-17 Indiana University Research And Technology Corporation Method for generating endothelial colony forming cell-like cells

Non-Patent Citations (24)

* Cited by examiner, † Cited by third party
Title
AU, P ET AL., BLOOD, vol. 111, 2008, pages 1302 - 1305
BOUVARD, C ET AL., ARTERIOSCLER THROMB VASE BIOL, vol. 30, 2010, pages 1569 - 1575
BROXMEYER, H.E. ET AL., BLOOD, vol. 117, 2011, pages 4773 - 4777
CALLONI ET AL.: "Reviewing and Updating the Major Molecular Markers for Stem Cells", STEM CELLS AND DEVELOPMENT, vol. 22, no. 9, 22 January 2013 (2013-01-22), pages 1455 - 1476, XP055240597 *
CRITSER, P.J. ET AL., MICRO VASE RES, vol. 80, 2010, pages 23 - 30
DUBOIS, C ET AL., J AM COLL CARDIOL, vol. 55, 2010, pages 2232 - 2243
GINSBERG ET AL., CELL, vol. 151, 2012, pages 559 - 575
INGRAM D.A., BLOOD, vol. 105, 2005, pages 2783 - 2786
INGRAM, D.A. ET AL., BLOOD, vol. 104, 2004, pages 2752 - 2760
LEE, J.H. ET AL., ARTERIOSCLER THROMB VASE BIOL, 2013
LEE, M.R. ET AL., STEM CELLS, vol. 31, 2013, pages 666 - 681
MEDINA, R.J. ET AL., INVEST OPHTHALMOL VIS SCI, vol. 51, 2010, pages 5906 - 5913
MELERO-MARTIN J.M., CIRC RES, vol. 103, 2008, pages 194 - 202
MOUBARIK, C ET AL., STEM CELL REV, vol. 7, 2011, pages 208 - 220
PRASAIN, N ET AL., NATURE BIOTECHNOLOGY, vol. 32, 2014, pages 1151 - 1157
ROBBINS, R.D. ET AL., CURR OPIN ORGAN TRANSPLANT, vol. 15, 2010, pages 61 - 67
SCHUH, A ET AL., BASIC RES CARDIOL, vol. 103, 2008, pages 69 - 77
SCHWARZ, T.M. ET AL., ARTERIOSCLER THROMB VASE BIOL, vol. 32, 2012, pages 13 - 21
STITT, A.W. ET AL., PROG RETIN EYE RES, vol. 30, 2011, pages 149 - 166
STRONCEK, J.D. ET AL., ACTA BIOMATER, vol. 8, 2012, pages 201 - 208
THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
VODYANIK ET AL., CELL STEM CELL, vol. 7, 2010, pages 718 - 729
YODER, M.C. ET AL., BLOOD, vol. 109, 2007, pages 1801 - 1809
YU ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3755354A4 (en) * 2018-02-21 2022-01-19 Indiana University Research and Technology Corporation COMPOSITIONS AND METHODS FOR THE TREATMENT OR PROPHYLAXIS OF A PERFUSION DISORDER
JPWO2021025083A1 (enExample) * 2019-08-05 2021-02-11
JP7184202B2 (ja) 2019-08-05 2022-12-06 日本製鉄株式会社 プレス成形品の製造方法、プレス成形品、およびプレス成形装置
WO2021176233A1 (en) * 2020-03-06 2021-09-10 Imperial College Innovations Limited Method of generating endothelial cells
WO2022040798A1 (en) * 2020-08-25 2022-03-03 University Health Network Compositions and methods for generating human yolk sac-like hematopoietic cells
CN115137740A (zh) * 2022-03-29 2022-10-04 广州大学 miRNA497b与miRNA-5106在制备治疗缺血心肌的药物中的应用
CN115137740B (zh) * 2022-03-29 2023-12-12 广州大学 miRNA-497b或miRNA-5106在制备治疗缺血心肌的药物中的应用

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US20230174929A1 (en) 2023-06-08
CN109689858A (zh) 2019-04-26
JP2019528057A (ja) 2019-10-10
BR112019002584A2 (pt) 2019-05-21
KR102456031B1 (ko) 2022-10-17
CA3033381A1 (en) 2018-02-15
JP7045363B2 (ja) 2022-03-31
AU2017308738A1 (en) 2019-01-31
IL264273A (en) 2019-02-28
IL264273B1 (en) 2024-04-01
US20190211304A1 (en) 2019-07-11
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IL264273B2 (en) 2024-08-01
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