JP7045363B2 - インビボで血管形成能を有する中胚葉細胞および/または血管内皮コロニー形成細胞様細胞を作製する方法 - Google Patents

インビボで血管形成能を有する中胚葉細胞および/または血管内皮コロニー形成細胞様細胞を作製する方法 Download PDF

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JP7045363B2
JP7045363B2 JP2019507218A JP2019507218A JP7045363B2 JP 7045363 B2 JP7045363 B2 JP 7045363B2 JP 2019507218 A JP2019507218 A JP 2019507218A JP 2019507218 A JP2019507218 A JP 2019507218A JP 7045363 B2 JP7045363 B2 JP 7045363B2
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シー. ヨーダー,マーヴィン
プラセン,ヌータン
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JP2019507218A 2016-08-10 2017-08-04 インビボで血管形成能を有する中胚葉細胞および/または血管内皮コロニー形成細胞様細胞を作製する方法 Active JP7045363B2 (ja)

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US201662372907P 2016-08-10 2016-08-10
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PCT/US2017/045496 WO2018031404A1 (en) 2016-08-10 2017-08-04 Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity

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WO2019164482A1 (en) * 2018-02-21 2019-08-29 Indiana University Research And Technology Corporation Compositions and methods for the treatment or prophylaxis of a perfusion disorder
CN114173952B (zh) * 2019-08-05 2024-08-13 日本制铁株式会社 冲压成型品的制造方法、冲压成型品以及冲压成型装置
MX2022002418A (es) * 2019-08-28 2022-03-22 Astellas Inst For Regenerative Medicine Composiciones y metodos de tratamiento de enfermedades vasculares.
GB202003309D0 (en) * 2020-03-06 2020-04-22 Imp College Innovations Ltd Methods of generating endothelial cells
WO2022040798A1 (en) * 2020-08-25 2022-03-03 University Health Network Compositions and methods for generating human yolk sac-like hematopoietic cells
CN115137740B (zh) * 2022-03-29 2023-12-12 广州大学 miRNA-497b或miRNA-5106在制备治疗缺血心肌的药物中的应用
CN116769710A (zh) * 2023-01-28 2023-09-19 深圳市寰宇生物科技有限公司 一种从人诱导性多能干细胞分化的巨噬细胞及其制备方法和应用
CN117070442B (zh) * 2023-10-18 2024-01-30 北京大学 静脉内皮细胞及其制备方法、静脉血管畸形模型及其制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100261274A1 (en) 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
WO2015138634A1 (en) 2014-03-11 2015-09-17 Indiana University Research And Technology Corporation Method for generating endothelial colony forming cell-like cells
US20160194610A1 (en) 2011-08-23 2016-07-07 Wisconsin Alumni Research Foundation Angiohematopoietic Progenitor Cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017390B2 (en) 2007-07-20 2011-09-13 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
WO2010011352A2 (en) * 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
CN104995294A (zh) * 2012-10-19 2015-10-21 新加坡科技研究局 使干细胞分化成一种或多种细胞谱系的方法
CA2906188A1 (en) * 2013-03-15 2014-09-18 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100261274A1 (en) 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
US20160194610A1 (en) 2011-08-23 2016-07-07 Wisconsin Alumni Research Foundation Angiohematopoietic Progenitor Cells
WO2015138634A1 (en) 2014-03-11 2015-09-17 Indiana University Research And Technology Corporation Method for generating endothelial colony forming cell-like cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Curr. Opin. Hematol.,2015年05月,Vol.22, No.3,pp.1-10(pp.252-257)
Nat. Biotechnol.,2014年11月,Vol.32, No.11,pp.1151-1157
Stem Cells Dev.,2013年05月01日,Vol.22, No.9,pp.1455-1476

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US20230174929A1 (en) 2023-06-08
CN109689858A (zh) 2019-04-26
JP2019528057A (ja) 2019-10-10
BR112019002584A2 (pt) 2019-05-21
KR102456031B1 (ko) 2022-10-17
CA3033381A1 (en) 2018-02-15
AU2017308738A1 (en) 2019-01-31
IL264273A (en) 2019-02-28
IL264273B1 (en) 2024-04-01
US20190211304A1 (en) 2019-07-11
CN109689858B (zh) 2024-07-26
IL264273B2 (en) 2024-08-01
WO2018031404A1 (en) 2018-02-15
JP2022081657A (ja) 2022-05-31
EP3497204A1 (en) 2019-06-19
EP3497204B1 (en) 2025-04-30
AU2017308738B2 (en) 2023-09-21
US11739293B2 (en) 2023-08-29
KR20190037299A (ko) 2019-04-05
EP3497204A4 (en) 2020-05-06

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