WO2017000702A1 - 荧光激发光源装置及系统和荧光显微成像系统 - Google Patents

荧光激发光源装置及系统和荧光显微成像系统 Download PDF

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WO2017000702A1
WO2017000702A1 PCT/CN2016/082769 CN2016082769W WO2017000702A1 WO 2017000702 A1 WO2017000702 A1 WO 2017000702A1 CN 2016082769 W CN2016082769 W CN 2016082769W WO 2017000702 A1 WO2017000702 A1 WO 2017000702A1
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light source
excitation light
source device
module
led module
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PCT/CN2016/082769
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English (en)
French (fr)
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夏浩涵
张莹莹
罗浦文
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上海睿钰生物科技有限公司
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Publication of WO2017000702A1 publication Critical patent/WO2017000702A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6478—Special lenses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements

Definitions

  • the present invention relates to the field of fluorescence analysis technology, and more particularly to a fluorescence excitation light source device and system and a fluorescence microscopy imaging system.
  • the fluorescence excitation light source device generally adopts a full-band light source such as a mercury lamp and a xenon lamp as a fluorescent excitation light source, so that the existing fluorescent excitation light source device has the disadvantages of high energy consumption, high cost and short life.
  • xenon lamps and mercury lamps can be used as white light sources. Although they can provide a range of wavelengths from ultraviolet to near-infrared, they have different excitation spectra. This high excitation energy at the peak ensures brightness. Fluorescent signal, but phototoxicity is very strong, which is not conducive to long-term live cell experiments.
  • the present invention provides a fluorescent excitation light source device and system and a fluorescence microscopic imaging system, which adopts a monochromatic LED module as a fluorescent excitation light source, and additionally cooperates with a concentrating module and a band pass filter.
  • the fluorescent excitation light source device has the advantages of short response time, low energy consumption, low cost, long service life, small volume, high light output brightness, and long-term living cell experiment.
  • the concentrating module is disposed between the monochromatic LED module and the band pass filter, and the adjustable fixing frame can adjust the monochromatic LED module and the concentrating module. The distance between them.
  • the concentrating module comprises a collecting lens or a lens group.
  • the single color LED module comprises at least one monochromatic LED lamp bead.
  • the adjustable fixing frame is a sealed adjustable fixing frame, and the single-color LED module, the concentrating module and the band pass filter are both sealed and fixed in the adjustable fixing frame.
  • the band pass filter is disposed between the monochrome LED module and the concentrating module, and the adjustable fixing frame can adjust the monochrome LED module and the concentrating module. The distance between them.
  • the concentrating module comprises a collecting lens or a lens group.
  • the single color LED module comprises at least one monochromatic LED lamp bead.
  • the adjustable fixing frame is a sealed adjustable fixing frame, and the single-color LED module, the concentrating module and the band pass filter are both sealed and fixed in the adjustable fixing frame.
  • the present invention also provides a fluorescence excitation light source system comprising at least one fluorescence excitation light source device, wherein the fluorescence excitation light source device is the above-described fluorescent excitation light source device.
  • the present invention also provides a fluorescence microscopy imaging system comprising a fluorescence excitation light source system, wherein the fluorescence excitation light source system is the above-described fluorescence excitation light source system.
  • the technical solution provided by the present invention has at least the following advantages:
  • the invention provides a fluorescence excitation light source device and system and a fluorescence microscopic imaging system, comprising: an adjustable fixing frame; and a monochrome LED module, a concentrating module and a belt fixed in the adjustable fixing frame a pass filter; wherein the concentrating module is disposed between the monochromatic LED module and the band pass filter, or the band pass filter is disposed in the monochromatic LED module and Between the concentrating modules, And the adjustable fixture is capable of adjusting a distance between the monochrome LED module and the concentrating module.
  • the technical solution provided by the present invention uses the LED module as a fluorescent excitation light source, and the fluorescent excitation light source is characterized by the short response time, low energy consumption, low cost, long service life and small volume of the LED.
  • the device has the advantages of short response time, low energy consumption, low cost, long service life and small volume. And, because the attenuation of LED light is fast and accurate, the phototoxicity can be greatly reduced under long-term living cell test, so that the fluorescence excitation light source device is beneficial to long-term living cell experiments.
  • the concentrating module is disposed at the front end of the monochromatic LED module, which not only makes the fluorescent excitation light source device have high brightness, but also can adjust the concentrating module and the monochromatic LED module through the adjustable fixing frame. The distance is adjusted to adjust the size of the light spot of the fluorescent excitation light source device, thereby adjusting the concentration of the excitation light source energy of the light emitted by the fluorescent excitation light source device.
  • a band pass filter is disposed at the front end of the monochromatic LED module to constrain the light emitting wavelength range of the fluorescent excitation light source device, thereby further improving the singularity of the fluorescent excitation light source emitted by the fluorescent excitation light source device.
  • FIG. 1 is a schematic structural view of a cut surface of a fluorescence excitation light source device according to an embodiment of the present application
  • FIG. 2 is a schematic structural diagram of a cut surface of a fluorescence excitation light source device according to another embodiment of the present application.
  • the fluorescence excitation light source device generally adopts a full-band light source such as a mercury lamp and a xenon lamp as a fluorescence excitation light source, so that the existing fluorescence excitation light source device has high energy consumption.
  • a full-band light source such as a mercury lamp and a xenon lamp
  • xenon lamps and mercury lamps can be used as white light sources. Although they can provide a range of wavelengths from ultraviolet to near-infrared, they have different excitation spectra. This high excitation energy at the peak ensures brightness. Fluorescent signal, but phototoxicity is very strong, which is not conducive to long-term live cell experiments.
  • the embodiment of the present application provides a fluorescent excitation light source device, which adopts a single-color LED (Light Emitting Diode) module as a fluorescent excitation light source, and additionally cooperates with a concentrating module and a band pass filter.
  • the fluorescent excitation light source device has the advantages of short response time, low energy consumption, low cost, long service life, small volume, high light output brightness, and long-term living cell experiment.
  • the fluorescence excitation light source device provided by the present application will be described in detail with reference to FIG. 1 and FIG. 2 in detail.
  • FIG. 1 a schematic diagram of a cross-sectional structure of a fluorescent excitation light source device according to an embodiment of the present invention, wherein the fluorescent excitation light source device includes:
  • Adjustable fixing frame 100
  • the concentrating module 300 is disposed between the monochromatic LED module 200 and the band pass filter 400, and the adjustable fixing frame 100 can adjust the distance between the monochromatic LED module 200 and the concentrating module 300.
  • the technical solution provided by the embodiment of the present application adopts the LED module as a fluorescent excitation light source, and the LED has the characteristics of short response time, low cost, long service life, small volume, etc., so that the fluorescent excitation light source device has Short response time, low cost, long service life and small size. And, because the attenuation of LED light is fast and accurate, the phototoxicity can be greatly reduced under long-term living cell test, so that the fluorescence excitation light source device is beneficial to long-term living cell experiments.
  • the LED since the LED only needs 3W of power to realize the same wavelength of fluorescence excitation light provided by the mercury lamp, the xenon lamp, etc., compared with the mercury lamp requires 50W to 100W, and the xenon lamp requires 100W of power, the LED has The low energy consumption makes the fluorescence excitation light source device greatly reduce the power consumption.
  • the concentrating module is disposed at the front end of the monochromatic LED module, which not only makes the fluorescent excitation light source device have high brightness, but also can adjust the concentrating module and the monochromatic LED module through the adjustable fixing frame. The distance between them is to adjust the size of the light spot of the fluorescent excitation light source device, thereby adjusting the concentration of the excitation light source energy of the light emitted by the fluorescent excitation light source device.
  • a band pass filter is disposed at the front end of the monochromatic LED module to constrain the light emitting wavelength range of the fluorescent excitation light source device, thereby further improving the singularity of the fluorescent excitation light source emitted by the fluorescent excitation light source device.
  • the blue LED module has a peak wavelength range of 470 ⁇ 15 nm (including the endpoint value), and its corresponding bandpass filter can pass light with a wavelength range of 470 ⁇ 15 nm (including the endpoint value), so The pass filter can concentrate the wavelength of the fluorescent excitation source emitted by the fluorescence excitation source device between 465 nm and 485 nm (including the endpoint value), further improving the unity of the fluorescent excitation source emitted by the fluorescent excitation source device.
  • the concentrating module provided by the embodiment of the present invention includes, but is not limited to, a concentrating lens or a lens group. That is, the concentrating module provided by the embodiment of the present application may be a single concentrating lens, or may be more A lens group having a condensing function, which is composed of a lens, is not specifically limited in this application, and needs to be specifically designed according to actual needs. Moreover, the material of the lens in the condensing lens and the lens group is also not limited in the present application.
  • the monochrome LED module provided by the embodiment of the present application includes at least one single-color LED lamp bead.
  • the number of the single-color LED lamp bead in the LED module specific design is required according to the actual application, and the present application does not specifically limit the application.
  • the adjustable fixing frame provided by the embodiment of the present application is a sealed adjustable fixing frame, and the monochrome LED module and the concentrating module and The band pass filters are sealed and fixed in the adjustable holder.
  • the monochromatic LED module, the concentrating module and the band pass filter are both sealed and fixed in the adjustable fixing frame, so that all the fluorescent excitation light sources emitted by the monochromatic LED module are incident on the concentrating module, and then pass through
  • the fluorescent excitation light source of the concentrating module is all incident on the band pass filter, and then filtered and then emitted, thereby avoiding waste before the light of the fluorescent excitation light source device, and improving the light intensity of the fluorescent excitation light source device.
  • the present application does not specifically limit the shape of the adjustable fixing frame.
  • the method for adjusting the distance between the monochrome LED module and the collecting lens of the adjustable fixing frame is not specifically limited, and The actual application is designed specifically.
  • the concentrating module is disposed between the monochromatic LED module and the band pass filter.
  • the concentrating module can also Set on the side of the bandpass filter that faces away from the monochrome LED module.
  • FIG. 2 a schematic diagram of a cross-sectional structure of a fluorescent excitation light source device according to another embodiment of the present application, wherein the fluorescent excitation light source device includes:
  • Adjustable fixing frame 100
  • the band pass filter 400 is disposed between the monochromatic LED module 200 and the concentrating module 300, and the adjustable mount 100 can adjust the distance between the monochromatic LED module 200 and the concentrating module 300.
  • the technical solution provided by the embodiment of the present application adopts the LED module as a fluorescent excitation light source, and the LED has the characteristics of short response time, low cost, long service life, small volume, etc., so that the fluorescent excitation light source device has Short response time, low cost, long service life and small size. And, because the attenuation of LED light is fast and accurate, the phototoxicity can be greatly reduced under long-term living cell test, so that the fluorescence excitation light source device is beneficial to long-term living cell experiments.
  • the LED since the LED only needs 3W of power to realize the same wavelength of fluorescence excitation light provided by the mercury lamp, the xenon lamp, etc., compared with the mercury lamp requires 50W to 100W, and the xenon lamp requires 100W of power, the LED has The low energy consumption makes the fluorescence excitation light source device greatly reduce the power consumption.
  • the concentrating module is disposed at the front end of the monochromatic LED module, which not only makes the fluorescent excitation light source device have high brightness, but also can adjust the concentrating module and the monochromatic LED module through the adjustable fixing frame. The distance is adjusted to adjust the size of the light spot of the fluorescent excitation light source device, thereby adjusting the concentration of the excitation light source energy of the light emitted by the fluorescent excitation light source device.
  • a band pass filter is disposed at the front end of the monochromatic LED module to constrain the light emitting wavelength range of the fluorescent excitation light source device, thereby further improving the singularity of the fluorescent excitation light source emitted by the fluorescent excitation light source device.
  • the blue LED module has a peak wavelength range of 470 ⁇ 15 nm (including the endpoint value), and its corresponding bandpass filter can pass light with a wavelength range of 470 ⁇ 15 nm (including the endpoint value), so The pass filter can concentrate the wavelength of the fluorescent excitation source emitted by the fluorescence excitation source device between 465 nm and 485 nm (including the endpoint value), further improving the unity of the fluorescent excitation source emitted by the fluorescent excitation source device.
  • the concentrating module provided by the embodiment of the present invention includes, but is not limited to, a concentrating lens or a lens group. That is, the concentrating module provided by the embodiment of the present application may be a single concentrating lens, or may be more A lens group having a condensing function, which is composed of a lens, is not specifically limited in this application, and needs to be specifically designed according to actual needs. Moreover, the material of the lens in the condensing lens and the lens group is also not limited in the present application.
  • the monochrome LED module provided by the embodiment of the present application includes at least one single-color LED lamp bead.
  • the number of the single-color LED lamp bead in the LED module specific design is required according to the actual application, and the present application does not specifically limit the application.
  • the adjustable fixing frame provided by the embodiment of the present application is a sealed adjustable fixing frame, and the monochrome LED module and the concentrating module and The band pass filters are sealed and fixed in the adjustable holder.
  • the monochromatic LED module, the concentrating module and the band pass filter are both sealed and fixed in the adjustable fixing frame, so that all the fluorescent excitation light sources emitted by the monochromatic LED module are incident on the concentrating module, and then pass through
  • the fluorescent excitation light source of the concentrating module is all incident on the band pass filter, and then filtered and then emitted, thereby avoiding waste before the light of the fluorescent excitation light source device, and improving the light intensity of the fluorescent excitation light source device.
  • the present application does not specifically limit the shape of the adjustable fixing frame.
  • the method for adjusting the distance between the monochrome LED module and the collecting lens of the adjustable fixing frame is not specifically limited, and The actual application is designed specifically.
  • the embodiment of the present application further provides a fluorescence excitation light source system, wherein the fluorescence excitation light source system comprises at least one fluorescence excitation light source device, wherein the fluorescence excitation light source device is the fluorescent excitation light source device provided by any of the above embodiments.
  • the embodiment of the present application further provides a fluorescence microscopy imaging system, which comprises a fluorescence excitation light source system, wherein the fluorescence excitation light source system is the fluorescence excitation light source system provided by any of the above embodiments.
  • Embodiments of the present application provide a fluorescence excitation light source device and system, and a fluorescence microscopy imaging system, including: an adjustable fixture; and a monochrome LED module fixed in the adjustable fixture, a concentrating module and a band pass filter; wherein the concentrating module is disposed between the monochromatic LED module and the band pass filter, or the band pass filter is disposed on the Between the monochromatic LED module and the concentrating module, and the adjustable fixing frame can adjust the distance between the monochromatic LED module and the concentrating module.
  • the technical solution provided by the embodiment of the present application uses the LED module as a fluorescent excitation light source, and the LED has the characteristics of short response time, low energy consumption, low cost, long service life, small volume, and the like.
  • the excitation light source device has the advantages of short response time, low energy consumption, low cost, long service life and small volume. And, because the attenuation of LED light is fast and accurate, the phototoxicity can be greatly reduced under long-term living cell test, so that the fluorescence excitation light source device is beneficial to long-term living cell experiments.
  • the concentrating module is disposed at the front end of the monochromatic LED module, which not only makes the fluorescent excitation light source device have high brightness, but also can adjust the concentrating module and the monochromatic LED module through the adjustable fixing frame. The distance is adjusted to adjust the size of the light spot of the fluorescent excitation light source device, thereby adjusting the concentration of the excitation light source energy of the light emitted by the fluorescent excitation light source device.
  • a band pass filter is disposed at the front end of the monochromatic LED module to constrain the light-emitting wavelength range of the fluorescent excitation light source device, thereby further improving the unity of the excitation light source of the fluorescent excitation light source device.

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Abstract

一种荧光激发光源装置及系统和荧光显微成像系统，包括：可调整固定架(100)；以及，固定于所述可调整固定架(100)中的单色LED模组(200)、聚光模组(300)和带通滤光片(400)；其中，所述聚光模组(300)设置于所述单色LED模组(200)和带通滤光片(400)之间，或者，所述带通滤光片(400)设置于所述单色LED模组(200)和聚光模组(300)之间，且所述可调整固定架(100)能够调整所述单色LED模组(200)和所述聚光模组(300)之间的距离。该荧光激发光源装置具有响应时间短、耗能低、成本低、使用寿命长、体积小、出光亮度高、利于长期活细胞实验、发出的荧光激发光源单一性高等优点。

Description

荧光激发光源装置及系统和荧光显微成像系统
本申请要求于2015年07月01日提交中国专利局、申请号为201510376530.0、发明名称为“集成式荧光激发光源装置”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
本申请还要求于2015年07月28日提交中国专利局、申请号为201510452276.8、发明名称为“荧光激发光源装置及系统和荧光显微成像系统”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及荧光分析技术领域，更为具体的说，涉及一种荧光激发光源装置及系统和荧光显微成像系统。
背景技术
目前，在荧光显微成像系统中，其荧光激发光源装置普遍采用汞灯和氙灯等全波段光源作为荧光激发光源，使现有的荧光激发光源装置具有能耗高、成本高、寿命短的缺点。尤其的，氙灯和汞灯均能够作为白光源，虽然能提供从紫外到近红外一系列波长的光，但它们却有着不同的激发光谱，这种在峰值的高激发能量虽然能确保产生明亮的荧光信号，但光毒性却很强，不利于长期活细胞实验。
发明内容
有鉴于此，本发明提供了一种荧光激发光源装置及系统和荧光显微成像系统，采用单色LED模组作为荧光激发光源，另外通过聚光模组和带通滤光片的配合，使得荧光激发光源装置具有响应时间短、耗能低、成本低、使用寿命长、体积小、出光亮度高、利于长期活细胞实验等优点。
为实现上述目的，本发明提供的技术方案如下：
一种荧光激发光源装置，包括：
可调整固定架；
以及，固定于所述可调整固定架中的单色LED模组、聚光模组和带通滤光片；
其中，所述聚光模组设置于所述单色LED模组和带通滤光片之间，且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
优选的，所述聚光模组包括聚光透镜或透镜组。
优选的，所述单色LED模组包括至少一个单色LED灯珠。
优选的，所述可调整固定架为密封可调整固定架，且所述单色LED模组、聚光模组和带通滤光片均密封固定于所述可调整固定架内。
以及，一种荧光激发光源装置，包括：
可调整固定架；
以及，固定于所述可调整固定架中的单色LED模组、聚光模组和带通滤光片；
其中，所述带通滤光片设置于所述单色LED模组和聚光模组之间，且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
优选的，所述聚光模组包括聚光透镜或透镜组。
优选的，所述单色LED模组包括至少一个单色LED灯珠。
优选的，所述可调整固定架为密封可调整固定架，且所述单色LED模组、聚光模组和带通滤光片均密封固定于所述可调整固定架内。
相应的，本发明还提供了一种荧光激发光源系统，所述荧光激发光源系统包括至少一个荧光激发光源装置，其中，所述荧光激发光源装置为上述的荧光激发光源装置。
相应的，本发明还提供了一种荧光显微成像系统，所述荧光显微成像系统包括荧光激发光源系统，其中，所述荧光激发光源系统为上述的荧光激发光源系统。
相较于现有技术，本发明提供的技术方案至少具有以下优点：
本发明提供了一种荧光激发光源装置及系统和荧光显微成像系统，包括：可调整固定架；以及，固定于所述可调整固定架中的单色LED模组、聚光模组和带通滤光片；其中，所述聚光模组设置于所述单色LED模组和带通滤光片之间，或者，所述带通滤光片设置于所述单色LED模组和聚光模组之间， 且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
由上述内容可知，本发明提供的技术方案，采用但是LED模组作为荧光激发光源，并且由于LED具有响应时间短、耗能低、成本低、使用寿命长、体积小等特点，使得荧光激发光源装置具有响应时间短、耗能低、成本低、使用寿命长、体积小等优点。以及，由于LED光的衰减即快又精确，长期活细胞试验下可大大减少光毒性，使得荧光激发光源装置利于长期活细胞实验。
另外，将聚光模组设置于单色LED模组的前端，不仅使荧光激发光源装置的出光亮度高，而且还能可以通过可调固定架调整聚光模组和单色LED模组之间的距离，以调节荧光激发光源装置的出光光斑大小，进而调节荧光激发光源装置的出光的激发光源能量的聚集程度。
此外，将带通滤光片设置于单色LED模组的前端，以约束荧光激发光源装置的出光波长范围，进一步提高荧光激发光源装置的发出的荧光激发光源的单一性。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据提供的附图获得其他的附图。
图1为本申请一实施例提供的一种荧光激发光源装置的切面结构示意图；
图2为本申请另一实施例提供的一种荧光激发光源装置的切面结构示意图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
正如背景技术所述，现有的在荧光显微成像系统中，其荧光激发光源装置普遍采用汞灯和氙灯等全波段光源作为荧光激发光源，使现有的荧光激发光源装置具有能耗高、成本高、寿命短的缺点。尤其的，氙灯和汞灯均能够作为白光源，虽然能提供从紫外到近红外一系列波长的光，但它们却有着不同的激发光谱，这种在峰值的高激发能量虽然能确保产生明亮的荧光信号，但光毒性却很强，不利于长期活细胞实验。
基于此，本申请实施例提供了一种荧光激发光源装置，采用单色LED(Light Emitting Diode，发光二极管)模组作为荧光激发光源，另外通过聚光模组和带通滤光片的配合，使得荧光激发光源装置具有响应时间短、耗能低、成本低、使用寿命长、体积小、出光亮度高、利于长期活细胞实验等优点。具体结合图1和图2所示，对本申请所提供的荧光激发光源装置进行详细的说明。
参考图1所示，为本申请一实施例提供的一种荧光激发光源装置的切面结构示意图，其中，荧光激发光源装置，包括：
可调整固定架100；
以及，固定于可调整固定架100中的单色LED模组200、聚光模组300和带通滤光片400；
其中，聚光模组300设置于单色LED模组200和带通滤光片400之间，且可调整固定架100能够调整单色LED模组200和聚光模组300之间的距离。
由上述内容可知，本申请实施例提供的技术方案，采用但是LED模组作为荧光激发光源，并且由于LED具有响应时间短、成本低、使用寿命长、体积小等特点，使得荧光激发光源装置具有响应时间短、成本低、使用寿命长、体积小等优点。以及，由于LED光的衰减即快又精确，长期活细胞试验下可大大减少光毒性，使得荧光激发光源装置利于长期活细胞实验。并且，由于LED仅仅需要3W的功率即可实现与汞灯、氙灯等提供的相同波段的荧光激发光，相较于汞灯需要50W至100W的功率，而氙灯需要100W的功率的情况，LED具有耗能低的特点，使得荧光激发光源装置大大降低了功耗。
另外，将聚光模组设置于单色LED模组的前端，不仅使荧光激发光源装置的出光亮度高，而且还能可以通过可调固定架调整聚光模组和单色LED模组之 间的距离，以调节荧光激发光源装置的出光光斑大小，进而调节荧光激发光源装置的出光的激发光源能量的聚集程度。
此外，将带通滤光片设置于单色LED模组的前端，以约束荧光激发光源装置的出光波长范围，进一步提高荧光激发光源装置的发出的荧光激发光源的单一性。例如，蓝色LED模组的峰值波长范围为470±15nm(包括端点值)，而与其对应的带通滤光片能够通过波长范围为470±15nm(包括端点值)的光，因此，通过带通滤光片能够使得荧光激发光源装置发出的荧光激发光源的波长集中在465nm～485nm(包括端点值)之间，进一步提高荧光激发光源装置的发出的荧光激发光源的单一性。
其中，本申请实施例提供的聚光模组包括但不限于聚光透镜或透镜组，即，本申请实施例提供的聚光模组可以为单独的一个聚光透镜，或者，还可以为多个透镜组成的具有聚光功能的透镜组，对此本申请不作具体限制，需要根据实际需要进行具体设计。而且，本申请对于聚光透镜和透镜组中透镜的材质同样不作限制。
此外，本申请实施例提供的单色LED模组包括至少一个单色LED灯珠，对于LED模组中单色LED灯珠的数量，需要根据实际应用进行具体设计，本申请不作具体限制。
进一步的，为了避免荧光激发光源装置中LED模组提供的荧光激发光源的浪费，本申请实施例提供的可调整固定架为密封可调整固定架，且单色LED模组、聚光模组和带通滤光片均密封固定于可调整固定架内。
即，将单色LED模组、聚光模组和带通滤光片均密封固定于可调整固定架内，保证单色LED模组发出的荧光激发光源全部入射至聚光模组，而后经过聚光模组的荧光激发光源全部入射至带通滤光片，而后通过经过滤波后出射，避免了荧光激发光源装置的出光之前的浪费，提高了荧光激发光源装置的出光强度。
需要说明的是，本申请对于可调整固定架的形状不作具体限制，另外，本申请对于可调整固定架调整单色LED模组和聚光透镜之间的距离的方式不作具体限定，均需要根据实际应用进行具体设计。
本申请上述实施例提供的荧光激发光源装置，其聚光模组设置于单色LED模组和带通滤光片之间；此外，在本申请另一实施例中，聚光模组还可以设置于带通滤光片背离单色LED模组的一侧。具体的，参考图2所示，为本申请另一实施例提供的一种荧光激发光源装置的切面结构示意图，其中，荧光激发光源装置，包括：
可调整固定架100；
以及，固定于可调整固定架100中的单色LED模组200、聚光模组300和带通滤光片400；
其中，带通滤光片400设置于单色LED模组200和聚光模组300之间，且可调整固定架100能够调整单色LED模组200和聚光模组300之间的距离。
由上述内容可知，本申请实施例提供的技术方案，采用但是LED模组作为荧光激发光源，并且由于LED具有响应时间短、成本低、使用寿命长、体积小等特点，使得荧光激发光源装置具有响应时间短、成本低、使用寿命长、体积小等优点。以及，由于LED光的衰减即快又精确，长期活细胞试验下可大大减少光毒性，使得荧光激发光源装置利于长期活细胞实验。并且，由于LED仅仅需要3W的功率即可实现与汞灯、氙灯等提供的相同波段的荧光激发光，相较于汞灯需要50W至100W的功率，而氙灯需要100W的功率的情况，LED具有耗能低的特点，使得荧光激发光源装置大大降低了功耗。
另外，将聚光模组设置于单色LED模组的前端，不仅使荧光激发光源装置的出光亮度高，而且还能可以通过可调固定架调整聚光模组和单色LED模组之间的距离，以调节荧光激发光源装置的出光光斑大小，进而调节荧光激发光源装置的出光的激发光源能量的聚集程度。
此外，将带通滤光片设置于单色LED模组的前端，以约束荧光激发光源装置的出光波长范围，进一步提高荧光激发光源装置的发出的荧光激发光源的单一性。例如，蓝色LED模组的峰值波长范围为470±15nm(包括端点值)，而与其对应的带通滤光片能够通过波长范围为470±15nm(包括端点值)的光，因此，通过带通滤光片能够使得荧光激发光源装置发出的荧光激发光源的波长集中在465nm～485nm(包括端点值)之间，进一步提高荧光激发光源装置的发出的荧光激发光源的单一性。
其中，本申请实施例提供的聚光模组包括但不限于聚光透镜或透镜组，即，本申请实施例提供的聚光模组可以为单独的一个聚光透镜，或者，还可以为多个透镜组成的具有聚光功能的透镜组，对此本申请不作具体限制，需要根据实际需要进行具体设计。而且，本申请对于聚光透镜和透镜组中透镜的材质同样不作限制。
此外，本申请实施例提供的单色LED模组包括至少一个单色LED灯珠，对于LED模组中单色LED灯珠的数量，需要根据实际应用进行具体设计，本申请不作具体限制。
进一步的，为了避免荧光激发光源装置中LED模组提供的荧光激发光源的浪费，本申请实施例提供的可调整固定架为密封可调整固定架，且单色LED模组、聚光模组和带通滤光片均密封固定于可调整固定架内。
即，将单色LED模组、聚光模组和带通滤光片均密封固定于可调整固定架内，保证单色LED模组发出的荧光激发光源全部入射至聚光模组，而后经过聚光模组的荧光激发光源全部入射至带通滤光片，而后通过经过滤波后出射，避免了荧光激发光源装置的出光之前的浪费，提高了荧光激发光源装置的出光强度。
需要说明的是，本申请对于可调整固定架的形状不作具体限制，另外，本申请对于可调整固定架调整单色LED模组和聚光透镜之间的距离的方式不作具体限定，均需要根据实际应用进行具体设计。
相应的，本申请实施例还提供了一种荧光激发光源系统，荧光激发光源系统包括至少一个荧光激发光源装置，其中，荧光激发光源装置为上述任意一实施例提供的荧光激发光源装置。
相应的，本申请实施例还提供了一种荧光显微成像系统，荧光显微成像系统包括荧光激发光源系统，其中，荧光激发光源系统为上述任意一实施例提供的荧光激发光源系统。
本申请实施例提供了一种荧光激发光源装置及系统和荧光显微成像系统，包括：可调整固定架；以及，固定于所述可调整固定架中的单色LED模组、 聚光模组和带通滤光片；其中，所述聚光模组设置于所述单色LED模组和带通滤光片之间，或者，所述带通滤光片设置于所述单色LED模组和聚光模组之间，且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
由上述内容可知，本申请实施例提供的技术方案，采用但是LED模组作为荧光激发光源，并且由于LED具有响应时间短、耗能低、成本低、使用寿命长、体积小等特点，使得荧光激发光源装置具有响应时间短、耗能低、成本低、使用寿命长、体积小等优点。以及，由于LED光的衰减即快又精确，长期活细胞试验下可大大减少光毒性，使得荧光激发光源装置利于长期活细胞实验。
另外，将聚光模组设置于单色LED模组的前端，不仅使荧光激发光源装置的出光亮度高，而且还能可以通过可调固定架调整聚光模组和单色LED模组之间的距离，以调节荧光激发光源装置的出光光斑大小，进而调节荧光激发光源装置的出光的激发光源能量的聚集程度。
此外，将带通滤光片设置于单色LED模组的前端，以约束荧光激发光源装置的出光波长范围，进一步提高荧光激发光源装置的出光的激发光源的单一性。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。

Claims (10)

  1. 一种荧光激发光源装置，其特征在于，包括：
    可调整固定架；
    以及，固定于所述可调整固定架中的单色LED模组、聚光模组和带通滤光片；
    其中，所述聚光模组设置于所述单色LED模组和带通滤光片之间，且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
  2. 根据权利要求1所述的荧光激发光源装置，其特征在于，所述聚光模组包括聚光透镜或透镜组。
  3. 根据权利要求1所述的荧光激发光源装置，其特征在于，所述单色LED模组包括至少一个单色LED灯珠。
  4. 根据权利要求1所述的荧光激发光源装置，其特征在于，所述可调整固定架为密封可调整固定架，且所述单色LED模组、聚光模组和带通滤光片均密封固定于所述可调整固定架内。
  5. 一种荧光激发光源装置，其特征在于，包括：
    可调整固定架；
    以及，固定于所述可调整固定架中的单色LED模组、聚光模组和带通滤光片；
    其中，所述带通滤光片设置于所述单色LED模组和聚光模组之间，且所述可调整固定架能够调整所述单色LED模组和所述聚光模组之间的距离。
  6. 根据权利要求5所述的荧光激发光源装置，其特征在于，所述聚光模组包括聚光透镜或透镜组。
  7. 根据权利要求5所述的荧光激发光源装置，其特征在于，所述单色LED模组包括至少一个单色LED灯珠。
  8. 根据权利要求5所述的荧光激发光源装置，其特征在于，所述可调整固定架为密封可调整固定架，且所述单色LED模组、聚光模组和带通滤光片均密封固定于所述可调整固定架内。
  9. 一种荧光激发光源系统，其特征在于，所述荧光激发光源系统包括至少一个荧光激发光源装置，其中，所述荧光激发光源装置为权利要求1～8任意 一项所述的荧光激发光源装置。
  10. 一种荧光显微成像系统，其特征在于，所述荧光显微成像系统包括荧光激发光源系统，其中，所述荧光激发光源系统为权利要求9所述的荧光激发光源系统。
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Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104990907B (zh) * 2015-07-01 2018-05-08 上海睿钰生物科技有限公司 自动多通道类流式图像荧光分析系统
CN105465745B (zh) * 2015-12-31 2019-04-05 中国科学院苏州生物医学工程技术研究所 多波段荧光照明装置
CA3011719A1 (en) 2016-01-21 2017-07-27 Protein Dynamic Solutions Llc Method and system for spectral data analysis
LU93022B1 (de) * 2016-04-08 2017-11-08 Leica Microsystems Verfahren und Mikroskop zum Untersuchen einer Probe
CN109803762B (zh) * 2016-10-07 2022-04-01 勃林格殷格翰维特梅迪卡有限公司 用于控制分析装置和分析系统的方法
CN107037576B (zh) * 2017-05-03 2023-08-15 中国科学院苏州生物医学工程技术研究所 光学显微成像设备及其成像方法
CN107367456B (zh) * 2017-07-20 2020-04-03 上海睿钰生物科技有限公司 一种免洗涤图像类流式荧光检测方法和系统
CN107356515B (zh) * 2017-07-20 2020-04-03 上海睿钰生物科技有限公司 一种荧光图像的荧光强度确定方法和系统
CN107272196A (zh) * 2017-08-03 2017-10-20 苏州天准科技股份有限公司 一种削弱远心光学系统环形光照明鬼影的方法
CN109932316A (zh) * 2017-12-18 2019-06-25 长光华大基因测序设备（长春）有限公司 基因测序光学装置
CN108318468B (zh) * 2018-05-05 2024-01-16 哈尔滨索飞永诚科技有限公司 一种用于对液体样品中荧光染料染色微粒进行快速计数的计数系统
CN109001900A (zh) * 2018-09-05 2018-12-14 南京大学 一种明场和荧光双模态的显微成像系统及方法
CN111041076B (zh) * 2018-10-11 2023-09-26 深圳华大生命科学研究院 气泡检测方法及系统、基因测序仪、计算机可读存储介质
CN111427142A (zh) 2019-01-09 2020-07-17 卡尔蔡司显微镜有限责任公司 用于显微镜设备的照明模块及相关控制方法和显微镜设备
CN111435192B (zh) * 2019-01-15 2021-11-23 卡尔蔡司显微镜有限责任公司 利用荧光显微镜生成荧光照片的方法
WO2020183433A1 (en) * 2019-03-14 2020-09-17 3M Innovative Properties Company Sterilization indicator reading apparatus with a color sensor
CN111239086B (zh) * 2019-08-30 2024-04-05 北京临近空间飞行器系统工程研究所 视觉背景装置，荧光显微光学系统，扫描分析系统
CN110866918B (zh) * 2019-12-11 2022-04-05 上海睿钰生物科技有限公司 酵母分析方法
CN110991379B (zh) * 2019-12-11 2022-02-22 上海睿钰生物科技有限公司 藻类计数方法
CN111024696B (zh) 2019-12-11 2022-01-11 上海睿钰生物科技有限公司 藻类分析方法
CN110987768B (zh) * 2019-12-11 2022-02-15 上海睿钰生物科技有限公司 酵母计数方法
CN111551531B (zh) * 2020-05-19 2023-04-18 北京金诺美科技股份有限公司 一种荧光激发系统及实时荧光定量pcr仪
CN113946043A (zh) * 2020-07-16 2022-01-18 香港科技大学 一种激发光源和包括该激发光源的荧光显微镜
CN112946673B (zh) * 2021-01-29 2023-01-06 上海睿钰生物科技有限公司 一种激光测距方法、对焦方法、激光测距系统及对焦系统
CN113092428B (zh) * 2021-04-02 2023-07-25 安图实验仪器(郑州)有限公司 一种多重荧光检测方法、装置、设备和系统
CN113109314A (zh) * 2021-05-28 2021-07-13 上海睿钰生物科技有限公司 多荧光信号检测系统和方法
CN114199849A (zh) * 2021-12-17 2022-03-18 广州博鹭腾生物科技有限公司 一种应用于荧光成像的荧光光路系统
CN114441496B (zh) * 2022-02-14 2024-03-01 江苏禹视科技有限公司 一种多光源荧光扫描分析系统和方法
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CN114813522B (zh) * 2022-06-29 2022-10-21 深圳安侣医学科技有限公司 基于显微放大数字图像的血液细胞分析方法及系统
CN117890580B (zh) * 2024-03-07 2024-05-24 南通戴尔诺斯生物科技有限公司 一种采用荧光标记的免疫层析检测设备及其使用方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020123132A1 (en) * 2000-01-17 2002-09-05 Takeo Tanaami Biochip reader
JP2004325174A (ja) * 2003-04-23 2004-11-18 Olympus Corp 蛍光検出装置
CN200968935Y (zh) * 2006-11-21 2007-10-31 杭州远方光电信息有限公司 用于发光二极管荧光粉测试的激发与接收装置
CN101191770A (zh) * 2006-11-21 2008-06-04 杭州远方光电信息有限公司 发光二极管荧光粉发射光谱测量方法
CN103645167A (zh) * 2013-12-13 2014-03-19 苏州东胜兴业科学仪器有限公司 一种聚合酶链反应检测仪
CN104949953A (zh) * 2015-07-01 2015-09-30 上海睿钰生物科技有限公司 荧光激发光源装置及系统和荧光显微成像系统
CN204945040U (zh) * 2015-07-28 2016-01-06 上海睿钰生物科技有限公司 荧光激发光源装置及系统和荧光显微成像系统
CN204945042U (zh) * 2015-07-28 2016-01-06 上海睿钰生物科技有限公司 自动多通道类流式图像荧光分析系统

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3164609B2 (ja) * 1990-10-31 2001-05-08 オリンパス光学工業株式会社 内視鏡装置
JP2000235002A (ja) * 1999-02-15 2000-08-29 Fuji Photo Film Co Ltd 撮影方法および撮影装置
GB0200938D0 (en) * 2002-01-16 2002-03-06 Solexa Ltd Prism design for scanning applications
CN2522855Y (zh) * 2002-01-30 2002-11-27 无锡市朗珈生物医学工程有限公司 带滤光片转盘的生物芯片荧光检测扫描装置
JP2005062023A (ja) * 2003-08-14 2005-03-10 Japan Science & Technology Agency 落射型蛍光測定装置
CN101072997A (zh) * 2003-08-14 2007-11-14 塞通诺米公司 用于粒子分类系统的光学检测器
JP4825426B2 (ja) * 2005-01-31 2011-11-30 財団法人 東京都医学総合研究所 生物顕微鏡に用いる暗視野照明装置
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
FR2889404B1 (fr) 2005-08-01 2009-03-27 Commissariat Energie Atomique Source lumineuse a deux longueurs d'onde et de puissance d'eclairement variable et utilisation d'une telle source lumineuse
GB0614297D0 (en) * 2006-07-19 2006-08-30 Shaw Water Engineering Ltd Apparatus, system and method for detecting particles
CN101149327B (zh) * 2007-11-06 2010-06-30 浙江大学 基于细胞显微图像信息的抗肿瘤药物评价和筛选方法
CN101363839B (zh) * 2008-09-12 2012-02-08 首都师范大学 可观测带荧光生物样品的非损伤微测系统
US8618508B2 (en) 2008-09-25 2013-12-31 Koninklijke Philips N.V. Detection system and method
JP2010134382A (ja) * 2008-12-08 2010-06-17 Olympus Corp 観察装置
WO2010101931A2 (en) * 2009-03-03 2010-09-10 Access Medical System Co., Ltd. Detection system and method for high sensitivity fluorescent assays
JP5499732B2 (ja) * 2009-06-23 2014-05-21 ソニー株式会社 生体サンプル像取得装置、生体サンプル像取得方法及び生体サンプル像取得プログラム
JP2011028187A (ja) * 2009-07-21 2011-02-10 Kenichi Tanehashi 実体顕微鏡のステージ上に置いて透過蛍光観察をする装置
PL2524221T3 (pl) * 2010-01-12 2022-06-20 Nexcelom Bioscience Llc System do zliczania komórek i biocząsteczek
CN102754011B (zh) * 2010-02-03 2015-08-05 株式会社尼康 观察装置及观察方法
JP2011186182A (ja) * 2010-03-09 2011-09-22 Claro Inc 顕微鏡装置
JP2011237549A (ja) * 2010-05-10 2011-11-24 Iponacology Ltd 顕微鏡照明装置における光源ユニット、およびそれを備える顕微鏡
DE102010023486A1 (de) 2010-06-11 2011-12-15 B. Braun Avitum Ag Nachweisvorrichtung und -verfahren
CN102329725A (zh) * 2010-06-16 2012-01-25 三星泰科威株式会社 光透射温度控制装置、生物诊断设备和方法
JP5707758B2 (ja) * 2010-07-13 2015-04-30 ソニー株式会社 撮像装置、撮像システム、手術用ナビゲーションシステム、及び撮像方法
EP2607889A4 (en) 2010-08-18 2016-11-09 Nanoentek Inc FLUORESCENCE MICROSCOPE FOR MULTI-FLUORESCENCE IMAGE OBSERVATION, FLUORESCENCE IMAGE OBSERVATION METHOD USING THE SAME, AND MULTI-FLUORESCENCE IMAGE OBSERVATION SYSTEM
JP2012168088A (ja) * 2011-02-16 2012-09-06 Ncd:Kk 蛍光反応検出装置
CN102151122B (zh) * 2011-03-17 2012-12-19 中国科学院自动化研究所 激发荧光分子成像系统及一次荧光成像方法
DE202011000688U1 (de) * 2011-03-25 2011-06-09 Leica Microsystems CMS GmbH, 35578 Vorrichtung zur Aufnahme von Filtern für Mikroskope
CN202057599U (zh) * 2011-05-17 2011-11-30 易定容 微多光谱荧光接收和处理系统
CN102305778B (zh) * 2011-05-17 2013-10-30 易定容 微多光谱荧光接收和处理系统
DE102011105181A1 (de) * 2011-06-17 2012-12-20 Leica Microsystems Cms Gmbh Mikroskop und Verfahren zur bildgebenden Fluoreszenzmikroskopie
DE102011051278A1 (de) * 2011-06-22 2012-12-27 Leica Microsystems Cms Gmbh Verfahren und lichtmikroskopische Einrichtung zur bildlichen Darstellung einer Probe
US20130015362A1 (en) * 2011-07-12 2013-01-17 Sharp Kabushiki Kaisha Fluid purification and sensor system
DE102011079942B4 (de) * 2011-07-27 2016-12-15 Leica Microsystems Cms Gmbh Mikroskopbeleuchtungsverfahren und Mikroskop
DE102011083215A1 (de) * 2011-09-22 2013-03-28 Siemens Aktiengesellschaft Probenträger und Verfahren zur mikroskopischen Untersuchung biologischer Proben
CN102507518B (zh) * 2011-10-25 2013-05-08 天津港东科技发展股份有限公司 12灯位多通道原子荧光光度计
CN103134780B (zh) * 2011-12-01 2015-12-09 中国科学院大连化学物理研究所 一种紧贴式激发光路的发光二极管诱导荧光检测器
JP5947553B2 (ja) * 2012-01-27 2016-07-06 浜松ホトニクス株式会社 撮像装置及び撮像装置の製造方法
DE102012201286A1 (de) * 2012-01-30 2013-08-01 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die wellenlängenselektive und örtlich hochauflösende Mikroskopie
US9395303B2 (en) 2012-03-12 2016-07-19 Mitsubishi Rayon Co., Ltd. Fluorescence detection device and fluorescence detection method
CN104641222A (zh) * 2012-05-29 2015-05-20 麦考瑞大学 用于发光显微成像的双向扫描
US8901514B2 (en) * 2012-06-28 2014-12-02 Molecular Devices, Llc Sample analysis system with spotlight illumination
CN103777336B (zh) * 2012-10-22 2017-09-05 承奕科技股份有限公司 显微光学撷取装置用荧光辅具模组、基架及该装置
US8785885B1 (en) 2013-01-30 2014-07-22 Omnivision Technologies, Inc. Fluorescence imaging module
CN103134784B (zh) * 2013-02-05 2015-04-29 华中科技大学 光纤化活体荧光激发光谱成像装置
WO2014191003A1 (en) * 2013-05-28 2014-12-04 Chemometec A/S Image forming cytometer
CN203337549U (zh) * 2013-06-28 2013-12-11 陕西师范大学 一种适用于微痕量爆炸物荧光检测的光学组件
CN203811540U (zh) * 2013-10-22 2014-09-03 闽浪仪器科技（厦门）有限公司 一种高度集成的多道数字荧光成像系统
CN104568859B (zh) * 2013-10-22 2017-10-13 承奕科技股份有限公司 具多组异角度光源的荧光观测装置、基架及荧光显微镜
CN103995346A (zh) * 2014-06-09 2014-08-20 武汉理工大学 一种显微镜物镜轴向扫描装置
CN204347334U (zh) * 2014-07-09 2015-05-20 闽浪仪器科技（厦门）有限公司 一种阵列式微滤片多道荧光显微镜
CN204330595U (zh) * 2014-07-15 2015-05-13 闽浪仪器科技（厦门）有限公司 一种实时多通道荧光检测系统
CN104198458B (zh) * 2014-09-26 2017-02-22 哈尔滨工业大学 一种飞秒激光双光子荧光生物显微成像系统及其成像方法
CN104296687A (zh) * 2014-11-05 2015-01-21 哈尔滨工业大学 基于荧光共焦显微技术的光滑大曲率样品测量装置与方法
CN204389397U (zh) * 2015-01-21 2015-06-10 南京中科神光科技有限公司 一种快速、多通道实时荧光定量检测装置
CN104677871A (zh) * 2015-02-27 2015-06-03 中国科学院自动化研究所 多光子激发光片照明显微成像系统
CN104730054B (zh) * 2015-04-10 2018-08-21 中国科学院烟台海岸带研究所 一种一体化探头式光电水质多参数在线测量系统
CN204789341U (zh) * 2015-07-01 2015-11-18 上海睿钰生物科技有限公司 集成式荧光激发光源装置
CN204945041U (zh) * 2015-07-28 2016-01-06 上海睿钰生物科技有限公司 一种多荧光通道同步显微成像装置

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020123132A1 (en) * 2000-01-17 2002-09-05 Takeo Tanaami Biochip reader
JP2004325174A (ja) * 2003-04-23 2004-11-18 Olympus Corp 蛍光検出装置
CN200968935Y (zh) * 2006-11-21 2007-10-31 杭州远方光电信息有限公司 用于发光二极管荧光粉测试的激发与接收装置
CN101191770A (zh) * 2006-11-21 2008-06-04 杭州远方光电信息有限公司 发光二极管荧光粉发射光谱测量方法
CN103645167A (zh) * 2013-12-13 2014-03-19 苏州东胜兴业科学仪器有限公司 一种聚合酶链反应检测仪
CN104949953A (zh) * 2015-07-01 2015-09-30 上海睿钰生物科技有限公司 荧光激发光源装置及系统和荧光显微成像系统
CN204945040U (zh) * 2015-07-28 2016-01-06 上海睿钰生物科技有限公司 荧光激发光源装置及系统和荧光显微成像系统
CN204945042U (zh) * 2015-07-28 2016-01-06 上海睿钰生物科技有限公司 自动多通道类流式图像荧光分析系统

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