WO2015119254A1 - 培養装置 - Google Patents
培養装置 Download PDFInfo
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- WO2015119254A1 WO2015119254A1 PCT/JP2015/053420 JP2015053420W WO2015119254A1 WO 2015119254 A1 WO2015119254 A1 WO 2015119254A1 JP 2015053420 W JP2015053420 W JP 2015053420W WO 2015119254 A1 WO2015119254 A1 WO 2015119254A1
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- incubator
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- housing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
Definitions
- This embodiment relates to a culture apparatus for culturing cells.
- a culture apparatus used for culturing cells is known.
- an incubator for keeping the temperature and the like constant is used.
- Japanese Patent Laid-Open No. 2000-37184 is known.
- An incubator provided with a processing means for performing a sterilization or cleaning process is disclosed.
- Japanese Patent Laid-Open No. 2000-93156 is known.
- a housing, a sample table on which a plurality of containers placed inside the housing and containing a sample are placed, and an environment for maintaining the environment inside the housing under a predetermined condition A control unit, a first opening opened in the housing, a first door that can be opened and closed to close the first opening, and the housing or the first door formed smaller than the first opening,
- An incubator is disclosed that includes a second opening having a size that allows at least one container to pass therethrough, and a second door that can be opened and closed to close the second opening.
- the present invention has been made in view of the above points, and provides a culture apparatus provided with an incubator that can make temperature, gas distribution, and the like in a housing uniform with high accuracy.
- the culture apparatus comprises: A culture device equipped with an incubator,
- the incubator part is An internal housing,
- a gas circulation unit that is installed at the center of the upper position in the inner casing, sucks in the gas in the inner casing, and discharges the sucked gas,
- a dispersion member that disperses the gas discharged from the gas circulation unit within the inner casing so as to be symmetric with respect to the symmetry axis;
- the dispersion member may disperse the gas discharged from the gas circulation unit in the internal casing so as to blow the gas toward each heating unit or above each heating unit.
- the incubator unit further includes a heat shield member disposed between the mounting unit and the heating unit, and disposed so as to surround at least a part of a peripheral edge of the mounting unit. A gap may be formed below the heat shield member between adjacent members.
- the incubator unit may further include a driving unit for rotating the placement unit about the symmetry axis.
- the incubator unit further includes an adjustment gas supply unit for supplying adjustment gas to the gas circulation unit, You may be able to adjust the density
- the culture apparatus according to the present invention comprises: You may further provide the adjustment gas sterilization part for disinfecting the adjustment gas supplied from the said adjustment gas supply part.
- the conditioning gas includes oxygen;
- the adjustment gas sterilization unit may irradiate the adjustment gas with ultraviolet rays.
- the incubator unit may further include a humidified water storage unit that is mounted below the mounting unit and stores humidification water.
- the incubator part may further include a detection part for detecting whether the container is placed on the placement part.
- the internal housing may have an opening / closing door installed corresponding to each placement unit.
- the number of the placing parts and the number of the doors may be the same.
- the incubator part further includes a holding part that extends in the vertical direction along the axis of symmetry and holds the mounting part in the horizontal direction
- the mounting unit is a first mounting unit held by the holding unit, and a second mounting unit that is positioned on the outer peripheral side of the first mounting unit and held by the first mounting unit. And an elastic member placed between the first placement portion and the second placement portion.
- the placement unit may be placed on a placement unit that is adjacent below in such a manner that the placement unit is movable in the vertical direction with respect to the placement unit adjacent below.
- the culture apparatus comprises: An external housing arranged to surround the internal housing; An external gas supply unit that is installed above the external housing and supplies gas into the external housing; And an external gas discharge unit that is installed below the external housing and discharges the gas supplied from the external gas supply unit.
- the culture apparatus comprises: An external housing arranged to surround the internal housing; You may further provide the sterilization part installed in the said external housing
- the sterilization part is a germicidal lamp
- a light shielding plate for preventing germicidal light emitted from the germicidal lamp from leaking out of the external housing may be installed below the germicidal lamp.
- the sterilization part is a germicidal lamp
- At least a part of the outer casing is transparent or translucent
- the portion of the outer casing that is transparent or translucent may be formed of a material that prevents sterilizing light emitted from the sterilizing lamp from leaking out of the outer casing.
- the culture apparatus comprises: A transfer case disposed adjacent to the internal case; A transport mechanism that is installed in the transport housing and takes the container in and out of the internal housing; And a vertical drive mechanism for moving the transport mechanism in the vertical direction.
- the mounting portion has a disk-shaped plate in which a plurality of arc-shaped gaps are formed concentrically, The arc-shaped gaps adjacent in the circumferential direction may be closed at a position where the container is to be placed to form a closed portion.
- the distance in the circumferential direction of the closing portion may be substantially the same as the distance in the circumferential direction of the container.
- FIG. 1 is a schematic top plan view showing a configuration of an automatic culture system according to an embodiment of the present invention.
- FIG. 2 is a control block diagram of the automatic culture system according to the embodiment of the present invention.
- FIG. 3 is a front view showing the container transport unit used in the embodiment of the present invention.
- FIG. 4 is a perspective view showing an external housing or the like surrounding the incubator used in the embodiment of the present invention.
- FIG. 5 is a perspective view showing a gas flow in the external housing used in the embodiment of the present invention.
- FIG. 6 is a perspective view showing a germicidal lamp, a light shielding plate and the like installed in the external housing used in the embodiment of the present invention.
- FIG. 7 is a perspective view showing an incubator section used in the embodiment of the present invention.
- FIG. 8 is a perspective view showing a state in which the heat insulating door of the incubator part used in the embodiment of the present invention is removed.
- FIG. 9 is a perspective view showing a mode in which the heat shielding member and the humidified water tray are removed from the mode shown in FIG.
- FIG. 10 is a side sectional view showing the structure inside the internal housing used in the embodiment of the present invention.
- FIG. 11A is an upper plan view of the mounting portion used in the embodiment of the present invention as viewed from above.
- FIG. 11B is an upper plan view of a modified example of the placement unit used in the embodiment of the present invention as viewed from above.
- FIG. 12 is a side cross-sectional view of a mounting portion and the like used in the embodiment of the present invention.
- FIG. 13 is a perspective view showing a detection unit and the like used in the embodiment of the present invention.
- FIG. 14 is a control block diagram relating to members related to the incubator according to the embodiment of the present invention.
- FIG. 15 is a side cross-sectional view illustrating an example in which a transport housing is disposed adjacent to the internal housing of the incubator unit.
- FIG. 16 is a graph showing measurement results of temperature pull-up characteristics according to examples and comparative examples of the present invention.
- FIG. 17 is a graph showing measurement results of temperature drop characteristics at the time of a power failure according to an example and a comparative example of the present invention.
- FIG. 18 is a graph showing measurement results of temperature recovery characteristics when the door is opened according to an example and a comparative example of the present invention.
- FIG. 19 is a graph showing measurement results of the return characteristics of the carbon dioxide concentration when the door is opened according to the example of the present invention and the comparative example.
- FIG. 1 to FIG. 19 are diagrams for explaining an embodiment of the present invention.
- the culture apparatus of the present embodiment can be used for culturing all types of cells, including pluripotent stem cells such as (human) iPS cells and (human) ES cells, and chondrocytes such as bone marrow stromal cells (MSC). It can be used when culturing various cells such as dendritic cells.
- pluripotent stem cells such as (human) iPS cells and (human) ES cells
- chondrocytes such as bone marrow stromal cells (MSC). It can be used when culturing various cells such as dendritic cells.
- MSC bone marrow stromal cells
- the automatic culture system of the present embodiment includes a raw material storage device 10 that stores raw material cells, and a container that transports a first sealed container 70 (see FIG. 3) that contains cells and the like in a sealed state.
- the second sealed container 75 that receives the transport unit 60 and the first sealed container 70 transported by the container transport unit 60 and accommodates cells from the first sealed container 70 (corresponds to a “container” in the claims).
- the following description will be given mainly on the premise that the “container” in the claims is the second sealed container 75, but the present invention is not limited to such an embodiment, and the dish for culture is used. It should be noted that various containers such as a culture dish and a culture dish can be used.
- the raw material storage device 10 includes an iPS cell establishment device 11 for establishing iPS cells.
- the raw material storage device 10 includes a unit thermostat, a centrifuge, an automatic blood cell counter, an automatic magnetic cell separator, a flow cytometer, a gene transfer device, and the like.
- the automatic culture apparatuses 20 and 30 automatically use a plurality (four in the embodiment shown in FIG. 1) of iPS cell automatic culture apparatuses 20 for automatically culturing iPS cells, and differentiated cells differentiated from iPS cells. And a plurality (8 in the embodiment shown in FIG. 1) of the differentiated cell automatic culturing apparatus 30.
- the iPS cell automatic culture apparatus 20 when simply referred to as “automatic culture apparatus”, the iPS cell automatic culture apparatus 20, the differentiated cell automatic culture apparatus 30, or the iPS cell automatic culture apparatus 20 and the differentiated cell automatic culture apparatus 30 are used. It means both.
- source cells such as somatic cells, iPS cells, differentiated cells, or source cells, iPS cells and iPS cells. It means any two or all of differentiated cells.
- the first sealed container 70 has a plurality of (eight in FIG. 3) shelves 71 on which the second sealed container 75 is placed, and each shelf 71 has a second sealed container 75. Is placed. Then, the first sealed container 70 is transported by the container transport unit 60 in a state where the plurality of second sealed containers 75 are accommodated in the first sealed container 70.
- the 2nd airtight container 75 may accommodate not only a cell but a liquid culture medium, a chemical
- the iPS cell automatic culture apparatus 20 includes a housing 22 as shown in FIG. 1, a medium analysis unit 24 for analyzing liquid medium components that change as the iPS cells are cultured, and iPS cells as shown in FIG.
- the cell inspection / removal unit 25 that inspects and removes iPS cells in a poor state, and stores and supplies a liquid medium or a liquid containing a proteolytic enzyme, and performs pretreatment before seeding iPS cells, or iPS An incubator that holds the liquid storage and supply unit 26 for seeding cells and collecting iPS cells and the second hermetic container 75 and automatically adjusts any one or all of temperature, humidity, and gas concentration Discharge for discharging the waste liquid including the used liquid medium, the used washing liquid, the used reagent and the like used in the unit 27 and the iPS cell automatic culture apparatus 20 downward from the housing 22 It has a 28, a.
- the iPS cell automatic culture device 20 also includes an in-device transport unit 23 that transports the second sealed container 75 and the like in the iPS cell automatic culture device 20.
- the liquid storage / supply unit 26 described above also has a function of inverting the second sealed container 75 upside down.
- the second sealed container 75 when used as in the present embodiment, it is not necessary to particularly manage the humidity in the incubator 27, so that the management of the cell culture environment can be simplified. .
- by adopting such a second hermetic container 75 there is no possibility of contamination from the air, and conveyance becomes easy.
- the above-described liquid storage and supply unit 26 automatically replaces the old liquid medium in the second sealed container 75 with a new liquid medium by appropriately supplying the liquid medium from the inflow port (not shown) into the second sealed container 75. Further, the cytological test removing unit 25 selectively separates defective iPS cells from an ECM (Extracellular matrix) applied to the surface of a film (not shown) of the second hermetic container 75 based on the acquired iPS cell information. . Thereafter, the liquid storage and supply unit 26 supplies the liquid medium from the inflow port into the second sealed container 75, thereby pushing out the suspended defective iPS cells from the second sealed container 75 through the unshown outlet.
- ECM Extracellular matrix
- a method for selectively detaching the iPS cells in the second sealed container 75 a method of applying ultrasonic waves or light to the iPS cells, a method of applying a physical force from the outside of the second sealed container 75, etc. Can be taken. Further, when using such a technique, a proteolytic enzyme may be used in combination.
- the liquid storage and supply unit 26 peels iPS cells from the ECM applied to the surface of the film 77 of the second sealed container 75 by appropriately supplying the proteolytic enzyme from the inflow port into the second sealed container 75. Thereafter, the liquid storage and supply unit 26 supplies the liquid medium from the inflow port into the second sealed container 75, whereby the suspended iPS cells are pushed out of the second sealed container 75 through the outflow port.
- the extruded iPS cells are diluted (made into a suspension) and then housed (seeded) in a plurality of second sealed containers 75. In this way, the iPS cell automatic culture apparatus 20 automatically performs the passage of iPS cells.
- the temperature in the iPS cell automatic culture apparatus 20 is adjusted by the incubator unit 27 so that the temperature becomes about 37 ° C., for example. Further, the gas concentration in the iPS cell automatic culture apparatus 20 is adjusted by the incubator unit 27 by appropriately adding carbon dioxide or nitrogen. Further, the humidity may be adjusted to about 100% by the incubator unit 37 as necessary.
- the differentiated cell automatic culture apparatus 30 includes a housing 32 as shown in FIG. 1, a medium analysis unit 34 for analyzing liquid medium components that change as the differentiated cells are cultured, and a differentiated cell as shown in FIG.
- a cell inspection / removal unit 35 that inspects and removes differentiated cells in a poor state, and stores and supplies a liquid medium, a liquid containing a proteolytic enzyme, and performs pretreatment before seeding differentiated cells, or differentiation.
- An incubator that holds a liquid storage and supply unit 36 for seeding cells and collecting differentiated cells and a second sealed container 75 and automatically adjusts any one or all of temperature, humidity, and gas concentration.
- the differentiated cell automatic culture device 30 also has an in-device transport unit 33 that transports the second sealed container 75 and the like in the differentiated cell automatic culture device 30.
- the liquid storage and supply unit 36 described above automatically replaces the old liquid medium in the second sealed container 75 with a new liquid medium by appropriately supplying the liquid medium from the inflow port to the second sealed container 75.
- the cell inspection removal unit 35 selectively peels off poorly differentiated cells from the ECM applied to the surface of the film 77 of the second sealed container 75 based on the acquired information of differentiated cells.
- the liquid storage and supply unit 36 supplies the liquid culture medium from the inflow port into the second sealed container 75, thereby pushing out the floating poorly differentiated cells from the second sealed container 75 through the outflow port.
- a method for selectively separating the differentiated cells in the second sealed container 75 a method of applying ultrasonic waves or light to the differentiated cells, a method of applying a physical force from the outside of the second sealed container 75, and the like. Can be taken. Further, when using such a technique, a proteolytic enzyme may be used in combination.
- the liquid storage and supply unit 36 peels the differentiated cells from the ECM applied to the surface of the film 77 of the second sealed container 75 by appropriately supplying the proteolytic enzyme from the inflow port into the second sealed container 75. Thereafter, the liquid storage and supply unit 36 supplies the liquid medium from the inflow port into the second sealed container 75, so that the suspended differentiated cells are pushed out of the second sealed container 75 through the outflow port.
- the differentiated cells that have been pushed out are diluted to a suspension and then housed (seeded) in a plurality of other second sealed containers 75. In this way, the differentiated cell automatic culture apparatus 30 automatically performs passage of differentiated cells.
- the temperature in the differentiated cell automatic culture apparatus 30 is adjusted by the incubator unit 37 so that the temperature becomes approximately 37 ° C., for example. Further, the gas concentration in the differentiated cell automatic culture apparatus 30 is adjusted by the incubator 37 by appropriately adding carbon dioxide or nitrogen. Note that the liquid storage and supply unit 36 of the differentiated cell automatic culture device 30 may supply a liquid medium containing a differentiation-inducing factor when inducing differentiation. Further, the humidity may be adjusted to about 100% by the incubator unit 37 as necessary.
- the iPS cell automatic culture apparatus 20 is connected to each of a medium analysis unit 24, a cell test removal unit 25, a liquid storage supply unit 26, an incubator unit 27, a discharge unit 28, and an in-device transport unit 23. And has a control unit 29 for controlling them.
- the control unit 29 manages the status, manages the log, manages the culture schedule, and performs a user interface function with respect to the iPS cell automatic culture apparatus 20.
- the differentiated cell automatic culture apparatus 30 is connected to and communicates with the medium analysis unit 34, the cell test removal unit 35, the liquid storage and supply unit 36, the incubator unit 37, the discharge unit 38, and the in-device transport unit 33.
- a control unit 39 manages the status, manages the log, manages the culture schedule, and performs the user interface function with respect to the differentiated cell automatic culture apparatus 30.
- the above-described iPS cell establishment device 11 has the same configuration as the iPS cell automatic culture device 20 and the differentiated cell automatic culture device 30. That is, the iPS cell establishment device 11 includes a housing 12b as shown in FIG. 1, a medium analysis unit 14 for analyzing a liquid medium as shown in FIG. Any one of the temperature inspection, the humidity, and the gas concentration in the housing 12b, or the cell storage / removal unit 15 for storing and supplying the liquid culture medium or the liquid containing the protease, etc. An incubator unit 17 that automatically adjusts all of these, and a waste liquid containing a used liquid medium, a used cleaning solution, a used reagent, and the like used in the iPS cell establishment device 11 are discharged downward from the housing 12b.
- the iPS cell establishment device 11 also includes an in-device conveyance unit 13 that conveys the second sealed container 75 and the like in the iPS cell establishment device 11.
- the iPS cell establishment device 11 is connected to and communicates with each of the medium analysis unit 14, the cell test removal unit 15, the liquid storage and supply unit 16, the incubator unit 17, the discharge unit 18, and the in-device transport unit 13.
- a control unit 19 is included.
- Each control unit 19, 29, 39 is connected to an external device 90 such as a personal computer.
- the container transport unit 60 of the present embodiment has a holding unit 61 that holds the first sealed container 70 so as to hang downward, and moves along a rail 65 provided on the ceiling. It is supposed to be.
- the iPS cell automatic culture apparatus 20 has a carry-in unit 21 for carrying a second closed container 75 from a first closed container 70.
- the carrying-in unit 21 carries the iPS cells housed in the second sealed container 75 and the cell loading / unloading part (not shown) for carrying out the cultured iPS cells and the second sealed container 75. And a material carrying-in part (not shown) for carrying in the used material.
- the differentiated cell automatic culture apparatus 30 has a carry-in unit 31 for carrying in the second closed container 75 from the first closed container 70.
- the carry-in unit 31 also carries the iPS cells housed in the second sealed container 75 and the cell carry-in / out part (not shown) for carrying out the cultured differentiated cells, and the second sealed container 75. And a material carrying-in part (not shown) for carrying in the used material.
- the materials are a liquid medium, a reagent, a washing solution, a culture plate, a vial, a filter, a needle, and the like.
- the iPS cell establishment device 11 also has a carry-in part 12 a for carrying in the first sealed container 70.
- the automatic culture system of the present embodiment includes a sterilization apparatus 1 for sterilizing the inside of the first sealed container 70 and iPS cells cultured in the iPS cell automatic culture apparatus 20 at a predetermined timing.
- An iPS cell analyzer 80 that receives from the carry-in unit 81 and inspects iPS cells, and a differentiated cell analyzer that receives the differentiated cells cultured in the differentiated cell automatic culture device 30 from the carry-in unit 86 at a predetermined timing and examines the differentiated cells.
- 85 and a cryopreservation apparatus 40 that receives iPS cells, differentiated cells, or both iPS cells and differentiated cells cultured in the automatic culture apparatuses 20 and 30 from the carry-in unit 41 and cryopreserves them.
- cryopreservation devices 40 may be provided, or the entire room may be cooled and the room itself may function as a freezer.
- a rail 65 is provided on the ceiling of the room, and a container transport unit is provided along the rail 65. 60 may be able to move.
- the sterilizing apparatus 1 As an example of the sterilizing apparatus 1 described above, an apparatus that sterilizes by supplying a sterilizing gas such as hydrogen peroxide gas or high-temperature gas into the first sealed container 70 can be cited. Moreover, as another example of the sterilization apparatus 1, the thing which sterilizes the inside of the 1st airtight container 70 by irradiating externally, for example with a gamma ray or an ultraviolet-ray, with the 1st airtight container 70 being a closed state can also be mentioned. . Incidentally, before the first sealed container 70 is carried in from the outside, the inside of the first sealed container 70 may be sterilized using, for example, ⁇ rays or ultraviolet rays. Note that the liquid medium or the like may contain proteins that are damaged by ⁇ rays or ultraviolet rays. In this case, it is desirable to sterilize with a sterilizing gas such as hydrogen peroxide gas or high-temperature gas.
- a sterilizing gas such as hydrogen peroxide gas or high-temperature
- the configuration near the above-described incubator will be described.
- any one, any two, or all of the incubator unit 17 of the iPS cell establishment device 11, the incubator unit 27 of the iPS cell automatic culture device 20, and the incubator unit 37 of the differentiated cell automatic culture device 30 will be described. This will be described as “incubator unit 100”.
- the incubator unit 17 of the iPS cell establishment device 11, the incubator unit 27 of the iPS cell automatic culture device 20, and the incubator unit 37 of the differentiated cell automatic culture device 30 have the same configuration.
- the incubator unit 100 has an internal housing 101 as shown in FIG. As shown in FIG. 10, the incubator 100 is provided in the inner casing 101 and corresponds to a central shaft 110 extending along an axis of symmetry extending in the vertical direction (corresponding to a “holding unit” in the claims). .), A plurality of (9 in the present embodiment) placement units 120 for placing the second sealed container 75 held in the horizontal direction by the center shaft 110 and containing cells, and the center shaft 110. It has a drive unit 130 such as a motor that rotates each placement unit 120 by rotating, and a plurality of heating units 140 installed in the internal housing 101. As shown in FIGS.
- the heating unit 140 is installed so as to be symmetric with respect to the symmetry axis, and more specifically, is placed at the four corners of the inner housing 101 (see FIG. 8). In FIG. 8 and FIG. 9, one heating unit 140 is hidden behind the mounting unit 120 or the like so that it cannot be seen.
- Each heating unit 140 of the present embodiment has a substantially U shape, and is a heater such as a fin heater, for example.
- each placement unit 120 As shown in FIG. 7, on the front side of the internal housing 101, it is installed corresponding to each placement unit 120, and a second sealed container 75 is placed on each placement unit 120 or each placement unit.
- An opening / closing door 105 for taking out the second hermetic container 75 from 120 is provided.
- nine opening / closing doors 105 are provided, and each opening / closing door 105 is positioned corresponding to each placement portion 120. Note that the number of placement units 120 and the number of doors 105 are the same (9 in this embodiment).
- the inner casing 101 described above has four heat insulating doors 101a.
- a mode in which the opening / closing door 105 is provided only on the heat insulating door 101a on the front surface is used.
- the present invention is not limited to this, and the opening / closing door 105 includes a plurality of heat insulating doors 101a (two heat insulating doors). 101a, three heat insulating doors 101a and four heat insulating doors 101a).
- the open / close door 105 is provided with an electromagnetic lock 106 (see FIG. 14).
- the heat insulating door 101a has a hybrid structure using two types of heat insulating materials, and one of the heat insulating materials is, for example, a vacuum heat insulating material.
- the mounting portion 120 of the present embodiment is held by a central shaft 110 and has a disk-shaped first mounting portion 121 having an open center and an outer periphery of the first mounting portion 121.
- a disc-shaped second mounting portion 122 that is located on the side and is held by an elastic member 123, which will be described later, by the first mounting portion 121 and has an open center, and the first mounting portion 121 and the second mounting portion.
- an elastic member 123 mounted between the mounting portion 122 and the mounting portion 122.
- each mounting part 120 will be mounted on the mounting part 120 which adjoins below, hold
- the arbitrary number of mounting parts 120 can be installed. For this reason, according to this Embodiment, the number of the mounting parts 120 can be adjusted as needed, and a cell can be cultured.
- each placement unit 120 is only placed on the lower placement unit 120 and is not fixed to the lower placement unit 120. It is possible to move in the vertical direction with respect to the mounting portion 120 to be moved. On the other hand, since the first placement portion 121 of each placement portion 120 is held by the center shaft 110, each first placement portion 121 cannot move in the horizontal direction.
- the present embodiment has a structure in which the mounting unit 120 is simply mounted on the mounting unit 120 adjacent to the lower side, and no extra wiring or piping is used.
- Cells can be cultured while rotating the second hermetic container 75 on the part 120.
- the detection unit 185 see FIG. 13
- the detection unit 185 is much more difficult than when determining whether the second sealed container 75 is mounted on the mounting unit 120. It can also be rotated at low speed. In this case, it is possible to suppress the cells from receiving vibration stress as much as possible while rotating the placement unit 120.
- the mounting portion 120 of the present embodiment has a disk-like plate in which a plurality of arc-shaped gaps are formed concentrically. Further, the arc-shaped gaps adjacent in the circumferential direction are closed at a position where the container is to be placed to form a closed portion. And as shown to FIG. 11B, the distance in the circumferential direction of this closing part may be substantially the same as the distance of the 2nd airtight container 75 at the time of measuring along the said circumferential direction.
- the 2nd airtight container 75 will be mounted on the mounting part 120 so that the film of the 2nd airtight container 75 may be positioned upwards. .
- the mounting part 120 has a positioning part for positioning the second sealed container 75, and this positioning part is composed of a plurality of protrusions 125 in the present embodiment. More specifically, the protrusions 125 are disposed such that the pair of protrusions 125 sandwich the corners of the four corners of the second sealed container 75 having a substantially rectangular shape.
- the second sealed container 75 can be placed at a predetermined position. As a result, even if a maximum number of the second sealed containers 75 are placed, Thus, gas can flow in the inner casing 101, and the temperature stability and temperature rise response in the inner casing 101 can be improved.
- this positioning part is composed of a plurality of protrusions 125 in the present embodiment. More specifically, the protrusions 125 are disposed such that the pair of protrusions 125 sandwich the corners of the four corners of the second sealed container 75 having a substantially rectangular shape.
- the second sealed container 75 can be placed at a predetermined position. As a result, even if a maximum number of the second sealed containers
- the second sealed container 75 may be placed on the placement unit 120 so that the film of the second sealed container 75 is positioned below, or the second sealed container 75 may be placed.
- the second hermetic container 75 may be placed on the placement portion 120 so that the film is positioned on the upper side.
- a DD motor direct drive motor
- a built-in Absolute encoder
- positioning can be performed with high accuracy, and infinite rotation can be performed with low vibration.
- the electric actuator using a stepping motor or a timing belt can also be employ
- the incubator unit 100 is installed at the central portion of the upper position in the internal housing 101, sucks the gas in the internal housing 101 from below, and discharges the sucked gas in the circumferential direction.
- a circulation unit 150 is included. Further, outside the periphery of the gas circulation unit 150, a gas discharged from the gas circulation unit 150 is dispersed in the inner casing 101 so as to be symmetric with respect to the symmetry axis, and a dispersion member (diffuser) 160 is provided. (See FIGS. 8 and 9).
- the dispersing member 160 moves the gas discharged from the gas circulation unit 150 in the circumferential direction so as to blow the gas toward the four corners of the inner casing 101 toward the upper side of each heating unit 140. It is designed to be distributed within the internal housing 101 (see FIG. 8).
- the dispersion member 160 not only applies pressure to the gas to be dispersed, but also functions as a guide vane that guides the gas.
- the gas circulation part 150 of this Embodiment consists of fans, such as a Siroko type fan, a centrifugal fan, an axial flow fan, for example.
- the dispersion member 160 will be described using a mode in which the gas discharged from the gas circulation unit 150 is blown toward the “upward” of each heating unit 140, but is not limited thereto.
- the dispersion member 160 may spray the gas discharged from the gas circulation unit 150 “directly” toward each heating unit 140.
- the incubator unit 100 further includes a heat shield member 170 installed between the mounting unit 120 and the heating unit 140 so as to surround the periphery of the mounting unit 120. Yes. A gap is formed below the heat shield member 170 between adjacent members, that is, the upper surface of the “bottom portion of the internal casing 101” in the present embodiment.
- a humidified water tray 180 (corresponding to “humidified water storage portion” in the claims) in which humidification water is stored is placed below the mounting portion 120 described above. Yes.
- the material of the humidifying water tray 180 for example, copper alloy stainless steel or copper alloy can be used. When such an aspect is employ
- gas is discharged from the gas circulation unit 150 (see FIG. 10). And the gas discharged
- the area is raised, the mounting part 120 and the second sealed container 75 are heated (see the dotted arrow in FIG. 8), and after the cells in the second sealed container 75 are heated, the gas circulation part 150 Inhaled. Then, the gas sucked from the gas circulation unit 150 in this manner is discharged from the gas circulation unit 150 again, and the above-described flow is repeated.
- the reason why the gas rises in the region on the inner peripheral side of the heat shield member 170 is that the gas is heated by the heating unit 140 and receives the suction force that the gas is sucked by the gas circulation unit 150.
- FIG. 8 there is a region where the heat shield member 170 is not disposed, but this region is located on the front surface side of the internal housing 101 and is opposed to the inner surface of the open / close door 105.
- the in-device transport units 13, 23, and 33 place the second hermetic container 75 on the placement units 120 in the internal housing 101 through the region where the heat shield member 170 is not disposed. In other words, the second sealed container 75 is taken out from the placement unit 120.
- the incubator unit 100 of the present embodiment includes a regulated gas supply unit 190 for supplying a regulated gas that is carbon dioxide and / or nitrogen, and a regulated gas supplied from the regulated gas supply unit 190. And an adjusted gas sterilization section (for example, an ultraviolet germicidal lamp) 195 for sterilizing the liquid with ultraviolet light or the like.
- an adjusted gas sterilization section for example, an ultraviolet germicidal lamp
- the adjustment gas which is carbon dioxide and / or nitrogen
- the adjustment gas sterilization unit 195 in this manner, microorganisms and bacteria contained in the adjustment gas can be sterilized.
- the adjustment gas contains oxygen to some extent, when an ultraviolet germicidal lamp is adopted as the conditioning gas sterilization unit 195, ozone gas is generated from oxygen by ultraviolet rays irradiated from the ultraviolet germicidal lamp, The inside of the incubator unit 100 can be always sterilized with the ozone gas.
- a guide pipe 111 for guiding the adjustment gas supplied from the adjustment gas supply unit 190 is disposed inside the central shaft 110 described above, and the adjustment gas that has passed through the guide pipe 111 passes through the gas circulation unit 150. It reaches down. Thus, the adjustment gas that has reached the lower side of the gas circulation unit 150 is sucked from the lower side of the gas circulation unit 150 and discharged to the outer periphery of the gas circulation unit 150. And the gas discharged
- FIG. The adjustment gas supply unit 190 and the guide tube 111 in the center shaft 110 are connected via a rotary joint 112.
- the incubator unit 100 of the present embodiment has a detection unit 185 for detecting whether the second sealed container 75 is placed on the placement unit 120.
- the detection unit 185 of the present embodiment is positioned above the uppermost mounting unit 120, and includes a light emitting unit 185a that emits light downward, and a lowermost mounting unit 120. And a light receiving unit 185b that receives light emitted from the light emitting unit 185a.
- this aspect is merely an example.
- the light emitting unit is positioned below the mounting unit 120 located at the lowest position, emits light upward, and receives light emitted from the light emitting unit.
- the light receiving unit may be positioned above the mounting unit 120 positioned at the uppermost position.
- An opening 129 is formed in a part of the mounting part 120, and the second hermetic container 75 is placed on at least a part of the opening 129 (FIGS. 11A and 11B). reference). For this reason, when the light emitted from the light emitting unit 185a passes through the mounting position of the second sealed container 75, if the light receiving unit 185b receives the light with an intensity equal to or higher than the threshold value, the second sealed container 75 is at that position. If the light receiving unit 185b does not receive the light or receives the light with an intensity less than a threshold value, the second sealed container 75 is placed at the position. Can be detected.
- the placement portion 120 since the placement portion 120 is held by the central shaft 110 so that the horizontal position of the opening 129 of each placement portion 120 matches, the placement portion 120 makes a round. Only it is possible to detect whether or not the second hermetic container 75 is placed on all the placement parts 120.
- an external housing 201 is provided so as to surround the internal housing 101 of the incubator unit 100.
- the outer casing 201 is open on the front side of the inner casing 101 where the open / close door 105 is installed. And the other apparatus which has the in-device conveyance parts 13, 23, and 33 will be connected with this open
- the external casing 201 of the present embodiment has four maintenance doors 201a, and each maintenance door 201a is removable. For this reason, for example, when maintaining the incubator part 100, these maintenance doors 201a can be removed, and it is very excellent in maintainability.
- an external gas supply unit 210 that supplies gas into the external housing 201 is provided above the external housing 201, and from the external gas supply unit 210 below the external housing 201.
- An external gas discharge unit 220 that discharges the supplied gas is provided.
- the external gas supply unit 210 is a fan filter unit (FFU), for example, and is a unit having a fan and a high-performance filter (HEPA filter). By adopting such a fan filter unit (FFU), the inside of the external housing 201 can be made a cleaner environment.
- the external gas discharge unit 220 is disposed on both sides of the internal housing 101 so that the gas in the external housing 201 can be exhausted in a balanced manner (FIG. 5). See arrow).
- the external gas discharge unit 220 has a louver (not shown), and by adjusting the opening degree of the louver, the speed and pressure of the gas flow in the external housing 201 can be adjusted. Yes.
- a sterilization unit including a sterilization lamp 230 for sterilizing the gas supplied from the external gas supply unit 210 is provided in the external housing 201.
- the sterilizing lamp 230 is disposed in the vicinity of the gas outlet 211 of the external gas supply unit 210, and sterilizes the gas by irradiating the gas blown from the external gas supply unit 210 with sterilizing light such as UV. Can be done.
- a mode in which the sterilization unit is composed of the sterilization lamp 230 is used.
- this is merely an example, and other sterilization modes can be used.
- the above-described maintenance door 201a is transparent or translucent, but a material that prevents the sterilizing light emitted from the sterilizing lamp 230 from leaking out of the outer casing 201 is used.
- the material of the maintenance door 201a is polycarbonate that cuts UV.
- a light shielding plate 240 is provided below the germicidal lamp 230 to prevent the germicidal light emitted from the germicidal lamp 230 from leaking out of the external housing 201. It is installed inclined at a certain angle.
- control units 19, 29, and 39 of the present embodiment include a drive unit 130, an opening / closing door 105, an electromagnetic lock 106, a gas circulation unit 105, an adjustment gas supply unit 190, a detection unit 185,
- the external gas supply unit 210, the external gas discharge unit 220, the germicidal lamp 230, and a notification unit 195, which will be described later, are communicatively connected to control them.
- the outer casing 201 is provided so as to surround the inner casing 101 of the incubator unit 100 as shown in FIGS.
- a delivery chamber 201 (corresponding to “carrying casing” in the claims) disposed adjacent to the inner casing 101, and installed in the delivery chamber 201, A transport mechanism 203 for taking the second sealed container 75 in and out of the delivery chamber 201 and a vertical drive mechanism 202 for moving the transport mechanism 203 in the vertical direction may be provided.
- the delivery chamber 201 of the present embodiment has a sealing property (air tightness) with respect to the outside air, and is airtightly connected to the internal housing 101 on the front surface side where the opening / closing door 105 of the internal housing 101 is installed. .
- the delivery chamber 201 is airtightly connected to the transfer chamber 301 having the in-device transport units 13, 23 and 33 and having a sealing property against the outside air.
- the in-device transport units 13, 23, and 33 are denoted by reference numeral 303.
- the second sealed container 75 when the second sealed container 75 is carried into the incubator unit 100, the second sealed container 75 is carried from the transfer chamber 301 to the delivery chamber 201 by the in-device transport unit 303, It is placed on the transport mechanism 203. Next, the second hermetic container 75 is moved in the vertical direction together with the transport mechanism 203 by the vertical drive mechanism 202 and stopped at a height position corresponding to the target opening / closing door 105. Then, after the target opening / closing door 105 is opened, the second hermetic container 75 is carried from the transfer chamber 301 to the internal housing 101 by the transport mechanism 203 and placed on the placement unit 120. When carrying out the 2nd airtight container 75 from the incubator part 100, the 2nd airtight container 75 is taken out from the mounting part 120 to the apparatus internal conveyance part 303 by performing the above-mentioned process in reverse order.
- the delivery chamber 201 is provided with a heating unit (not shown), and the atmosphere in the delivery chamber 201 is temperature-controlled to be 37 ° C., for example.
- a heating unit for example, a heater such as a fin heater can be used.
- an H 2 O 2 gas supply unit (not shown) is connected to the delivery chamber 201 so that the inside of the delivery chamber 201 can be sterilized periodically using H 2 O 2 gas. .
- the inside of the internal housing 101 of the incubator unit 100 can be prevented from being contaminated by the atmosphere in the delivery chamber 201.
- the plurality of heating units 140 are arranged so as to be symmetric with respect to the symmetry axis (see FIG. 10) extending in the vertical direction, and are dispersed.
- the member 160 disperses the gas discharged from the gas circulation unit 150 in the inner casing 101 so as to be symmetric with respect to the symmetry axis. For this reason, the temperature, gas distribution, etc. in the internal housing 101 of the incubator 100 can be made uniform with high accuracy. As a result, the cells in the second sealed container 75 can be cultured in a stable state.
- the dispersion member 160 disperses the gas discharged from the gas circulation unit 150 in the inner casing 101 so as to blow the gas upward of each heating unit 140. For this reason, since the gas discharged
- a heat shield member 170 is provided between the mounting unit 120 and the heating unit 140 so as to surround the periphery of the mounting unit 120. For this reason, it is possible to prevent the heating unit 140 from directly heating the second sealed container 75 and thus the cells in the second sealed container 75. For this reason, in this Embodiment, a cell can be cultured more reliably at a uniform temperature.
- the heat shield member 170 a member having at least an inner surface that is a mirror surface made of stainless steel, for example, can be used.
- heat from the inner peripheral side of the heat shield member 170 can be reflected to the inner peripheral side, and the cells in the second sealed container 75, and hence the second sealed container 75, can be efficiently used.
- the heating unit 140 is formed of an infrared heater or the like that emits infrared rays
- the entire heat shield member 170 is heated by infrared rays, and infrared rays on the inner peripheral side of the heat shield member 170 are transmitted to the heat shield member 170. Reflected by the mirror surface, the cells in the second sealed container 75 can be efficiently heated.
- the heat shield member 170 is detachable from the internal housing 101 (see FIG. 9). In FIG. 9, the humidified water tray 180 is also removed.
- the cells can be cultured while rotating the second hermetic container 75 on the placement unit 120 by the driving unit 130. For this reason, even if there is a bias in temperature, gas distribution, humidity, etc. in the inner housing 101 for some reason, the cells can be cultured under more uniform conditions.
- the incubator unit 100 has a regulated gas supply unit 190 for supplying a regulated gas that is nitrogen and / or carbon dioxide, as shown in FIG.
- a regulated gas supply unit 190 for supplying a regulated gas that is nitrogen and / or carbon dioxide, as shown in FIG.
- the adjustment gas supplied from the adjustment gas supply unit 190 reaches the lower part of the gas circulation unit 150 and is sucked from the lower part of the gas circulation unit 150 to the outer periphery of the gas circulation unit 150. Discharged. And the gas discharged
- the opening / closing door 105 shown in FIG. 7 is installed corresponding to each placement unit 120, and the second sealed container 75 is placed on each placement unit 120 via each opening / closing door 105.
- the second airtight container 75 can be taken out from the top 120. For this reason, it is possible to open only the opening / closing door 105 required when the in-device transport units 13, 23, and 33 access the incubator unit 100, and the in-device transport units 13, 23, and 33 access the incubator unit 100. By doing so, it is possible to suppress as much as possible that the temperature, the gas component, the humidity, and the like in the internal housing 101 are changed.
- the second sealed container 75 is mounted on each mounting unit 120 or the second sealing container 120 is sealed from the second mounting unit 120.
- the container 75 can be taken out. For this reason, compared with the aspect which the mounting part 120 moves up and down, the stress concerning the cell currently cultured can be reduced and it can also prevent that the atmosphere in the internal housing
- the incubator unit 100 of the present embodiment has a detection unit 185 for detecting whether the second sealed container 75 is mounted on the mounting unit 120 (see FIG. 13). For this reason, it is possible to prevent the incubator unit 100 from being sterilized at a high temperature while the second sealed container 75 is mounted on the mounting unit 120.
- the detection part 185 detect automatically whether the 2nd airtight container 75 is mounted on the mounting part 120.
- the mounting unit 120 is always rotated by the driving unit 130 while light is emitted from the light emitting unit 185a, and the second sealed container 75 is mounted on the mounting unit 120. You may make it detect whether it is carried out. If the detection unit 185 determines that the second sealed container 75 is placed on the placement unit 120, a notification unit 195 (see FIG. 14) and an external device 90 (see FIG. 2). ) Or the like, or the notification unit 195, the external device 90, or the like may be notified that high temperature sterilization cannot be performed.
- the elastic member 123 is placed between the first placement portion 121 and the second placement portion 122.
- each mounting part 120 is only mounted on the mounting part 120 which adjoins below, and can move to an up-down direction with respect to the mounting part 120 adjacent below. For this reason, even if the mounting part 120 and the center shaft 110 expand
- the incubator unit 100 instead of sterilizing the incubator unit 100 at a high temperature, it is also possible to sterilize the incubator unit 100 using H 2 O 2 gas periodically.
- the sterilization of the incubator unit 100 using H 2 O 2 gas is performed in a state where the second sealed container 75 is not placed on the placement unit 120 as in the high temperature sterilization.
- a humidifying water tray 180 containing humidifying water is placed below the placing portion 120. For this reason, the inside of the internal housing 101 can be kept at high humidity. Note that when the second sealed container 75 is used as described mainly so far, there is no need to keep the inside of the inner casing 101 at high humidity as described above. In the case where a culture dish or a culture dish is used instead of the container 75, it is very important to keep the inside of the internal casing 101 at high humidity in this way.
- the external housing 201 is disposed so as to surround the internal housing 101.
- An external gas supply unit 210 that supplies gas into the external housing 201 is provided above the external housing 201, and the gas supplied from the external gas supply unit 210 is disposed below the external housing 201.
- An external gas discharge unit 220 for discharging is provided.
- the incubator part 100 can be arrange
- a fan filter unit is employ
- the incubator part 100 can be arrange
- a sterilizing unit including a sterilizing lamp 230 that sterilizes the gas supplied from the external gas supply unit 210 is provided (see FIG. 6).
- the gas sterilized by the germicidal lamp 230 can be supplied from above the incubator unit 100. Therefore, the incubator unit 100 can be arranged in a cleaner environment, and contamination risk can be further suppressed.
- a light shielding plate 240 is installed below the germicidal lamp 230 to prevent the germicidal light emitted from the germicidal lamp 230 from leaking out of the external housing 201. Yes. For this reason, it can prevent beforehand that the sterilization light irradiated from the sterilization lamp 230 leaks outside, and affects the 2nd airtight container 75 grade
- the maintenance door 201a is made of polycarbonate or the like, and is made of a material that prevents the sterilizing light emitted from the sterilizing lamp 230 from leaking out of the external housing 201. For this reason, it can prevent more reliably beforehand that germicidal light, such as UV irradiated from the germicidal lamp 230, leaks outside and influences a human body etc.
- a delivery chamber 201 disposed adjacent to the inner casing 101 and a transport mechanism that is installed in the delivery chamber 201 and takes in and out the second sealed container 75 between the delivery chamber 201 and the delivery chamber 201.
- 203 and a vertical drive mechanism 202 that moves the transport mechanism 203 in the vertical direction.
- the transfer chamber 301 having the in-apparatus transport unit 303 is connected via the delivery chamber 201 instead of being directly connected to the internal housing 101 of the incubator unit 100, thereby further reducing environmental changes in the internal housing 101. It is possible to prevent bacteria from being brought in (contamination) from outside.
- the vertical drive mechanism 202 is provided in the delivery chamber 201, it is not necessary to provide the vertical drive mechanism in the in-device transport unit 303, and the mechanism (configuration) on the in-device transport unit 303 side can be simplified.
- Example An example of the incubator unit 100 of the culture apparatus according to the present embodiment will be described.
- the temperature performance was measured using the incubator unit 100 of the culture apparatus according to the present embodiment. For 30 minutes after the temperature in the internal casing 101 was stabilized, temperature fluctuation was measured at 1-minute intervals.
- the temperature in the inner casing 101 is set to 37.0 ° C.
- the temperature fluctuation range is 37.0 ⁇ 0.02 ° C.
- the temperature distribution is 37.0 ⁇ 0. It became 2 degreeC and the space temperature deviation became 0.20 degreeC.
- the temperature fluctuation range was 37.45 ⁇ 0.05 ° C.
- the temperature distribution was 37.45 ⁇ 0.32 ° C.
- the space temperature deviation was 0.54 ° C.
- JTM K01 / 03/05 is referred to as the temperature fluctuation range test standard
- JTM K01 / 03/05 is referred to as the temperature distribution test standard
- JTM K07 / 09 was used as a reference.
- FIG. 16 shows the result. What is indicated by a solid line is a measurement result by the incubator unit 100 of the present embodiment, what is indicated by a dotted line is a measurement result by a commercially available water jacket incubator, and what is indicated by a one-dot chain line is by a commercially available air jacket incubator. It is a measurement result.
- the incubator unit 100 of the present embodiment it can be understood that the temperature rises quickly and the time until temperature stabilization is short. In addition, since the time until temperature stabilization is short in this way, it can be understood that the temperature followability is high against disturbance.
- FIG. 17 shows the result.
- the solid line indicates the measurement result by the incubator unit 100 of the present embodiment
- the dotted line indicates the measurement result by the commercially available water jacket incubator, which is indicated by a one-dot chain line. What is measured by a commercially available air jacket incubator.
- the incubator unit 100 of the present embodiment it can be understood that the heat insulation performance is much higher than that of the air jacket incubator, and the heat retention performance is almost the same as that of the water jacket incubator. .
- FIG. 18 shows the result.
- the solid line indicates the measurement result by the incubator unit 100 of the present embodiment
- the dotted line indicates the measurement result by the commercially available water jacket incubator, which is indicated by a one-dot chain line. What is measured by a commercially available air jacket incubator.
- the incubator unit 100 of the present embodiment although the temperature rapidly drops when the door is opened in both the water jacket incubator and the air jacket incubator, for a long time of 60 seconds, It can be understood that the temperature was not affected even when the opening / closing door 105 was opened.
- the sealed container even when the sealed container is carried in and out, only the minimum necessary opening can be communicated to the outside, and a rapid change in temperature can be eliminated. Stress can be reduced as much as possible.
- the temperature does not drop even if the opening / closing door 105 is opened for a long time of 60 seconds, energy saving can be achieved, and it can be understood that it is resistant to disturbance. .
- the time of 60 seconds described above corresponds to the time required when, for example, the second sealed container 75 that proceeds to the next process is carried in and out.
- FIG. 19 shows the result.
- the solid line indicates the measurement result by the incubator unit 100 of the present embodiment
- the dotted line indicates the measurement result by the commercially available water jacket incubator, which is indicated by a one-dot chain line. What is measured by a commercially available air jacket incubator.
- the incubator unit 100 of the present embodiment although the carbon dioxide concentration suddenly drops when the door is opened in both the water jacket incubator and the air jacket incubator, In the meantime, it can be understood that the carbon dioxide concentration was not affected even when the opening / closing door 105 was opened. Since the carbon dioxide concentration does not decrease even when the door 105 is opened for a long time as described above, according to the incubator unit 100 of the present embodiment, energy saving can be achieved, and it is also strong against disturbance. Understandable.
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Abstract
Description
インキュベータ部を備えた培養装置であって、
前記インキュベータ部は、
内部筐体と、
前記内部筐体内に設置され、細胞を収容した容器を載置するための載置部と、
前記内部筐体内に設置された複数の加熱部であって、上下方向に延びた対称軸に対して対称となるように設置された複数の加熱部と、
前記内部筐体内の上方位置の中央部に設置され、前記内部筐体内の気体を吸い込み、吸い込んだ前記気体を排出する気体循環部と、
前記気体循環部から排出された気体を、前記対称軸に対して対称となるように、前記内部筐体内で分散させる分散部材と、
を有する。
前記分散部材は、各加熱部又は各加熱部の上方に向かって気体を吹き付けるように、前記気体循環部から排出された気体を前記内部筐体内で分散させてもよい。
前記インキュベータ部は、前記載置部と前記加熱部との間であって、前記載置部の周縁の少なくとも一部を取り囲むようにして設置された遮熱部材をさらに有し、
前記遮熱部材の下方には、隣接する部材との間に間隙が形成されてもよい。
前記インキュベータ部は、前記載置部を前記対称軸を中心に回転させるための駆動部をさらに有してもよい。
前記インキュベータ部は、前記気体循環部に、調整気体を供給するための調整気体供給部をさらに有し、
前記内部筐体内の気体内の前記調整気体の濃度を調整できてもよい。
前記調整気体供給部から供給される調整気体を殺菌するための調整気体殺菌部をさらに備えてもよい。
前記調整気体は酸素を含み、
前記調整気体殺菌部は前記調整気体に対して紫外線を照射してもよい。
前記インキュベータ部は、前記載置部の下方に載置され、加湿用の水を収容する加湿水収容部をさらに有してもよい。
前記インキュベータ部は、前記容器が前記載置部上に載置されているかを検知するための検知部をさらに有してもよい。
前記載置部は複数設けられており、
前記内部筐体は、各載置部に対応して設置された開閉扉を有してもよい。
前記載置部の数と前記開閉扉の数とは同数になっていてもよい。
前記インキュベータ部は、前記対称軸に沿って上下方向で延在し、前記載置部を水平方向で保持する保持部をさらに有し、
前記載置部は、前記保持部で保持された第一載置部と、前記第一載置部の周縁外方側に位置し、前記第一載置部で保持された第二載置部と、前記第一載置部と前記第二載置部との間に載置された弾性部材と、を有してもよい。
前記載置部は複数設けられ、
前記載置部は、下方で隣接する載置部に対して上下方向で移動可能となるようにして、下方で隣接する載置部上に載置されてもよい。
前記内部筐体を取り囲むようにして配置された外部筐体と、
前記外部筐体内の上方に設置され、前記外部筐体内に気体を供給する外部気体供給部と、
前記外部筐体内の下方に設置され、前記外部気体供給部から供給された気体を排出する外部気体排出部と、をさらに備えてもよい。
前記内部筐体を取り囲むようにして配置された外部筐体と、
前記外部筐体内に設置され、前記供給部から供給される気体を殺菌するための殺菌部をさらに備えてもよい。
前記殺菌部は殺菌灯であり、
前記殺菌灯の下方には、前記殺菌灯から照射される殺菌光が前記外部筐体外に漏れ出すことを防止するための遮光板が設置されてもよい。
前記殺菌部は殺菌灯であり、
前記外部筐体の少なくとも一部は透明又は半透明となっており、
前記外部筐体のうち透明又は半透明となっている箇所は前記殺菌灯から照射される殺菌光が前記外部筐体外に漏れ出すことを防止する材質から形成されてもよい。
前記内部筐体に隣接して配置された搬送用筐体と、
前記搬送用筐体内に設置され、前記内部筐体との間で前記容器を出し入れするための搬送機構と、
前記搬送機構を上下方向に移動させる上下駆動機構と、をさらに備えてもよい。
前記載置部は、同心円で複数の円弧状の隙間が形成された円盤状の板を有し、
円周方向において隣接する円弧状の隙間は、前記容器が載置される予定の位置で閉じて閉鎖部を形成してもよい。
前記閉鎖部の前記円周方向における距離は前記容器の前記円周方向における距離と略同一となってもよい。
《構成》
以下、本発明に係る培養装置の実施の形態について、図面を参照して説明する。ここで、図1乃至図19は本発明の実施の形態を説明するための図である。
まず、本実施の形態による自動培養システムの装置構成について説明する。
次に、上述したインキュベータ部近辺の構成について説明する。なお、以下では、iPS細胞樹立装置11のインキュベータ部17、iPS細胞自動培養装置20のインキュベータ部27及び分化細胞自動培養装置30のインキュベータ部37のいずれか1つ、いずれか2つ、又は、全てのことを「インキュベータ部100」と示して説明する。なお、本実施の形態では、iPS細胞樹立装置11のインキュベータ部17、iPS細胞自動培養装置20のインキュベータ部27及び分化細胞自動培養装置30のインキュベータ部37の各々は同様の構成となっている。
次に、上述した構成からなる本実施の形態によって達成される効果であって、まだ述べていない効果又はとりわけ重要な効果について説明する。
本実施の形態による培養装置のインキュベータ部100の実施例について説明する。
Claims (20)
- インキュベータ部を備えた培養装置であって、
前記インキュベータ部は、
内部筐体と、
前記内部筐体内に設置され、細胞を収容した容器を載置するための載置部と、
前記内部筐体内に設置された複数の加熱部であって、上下方向に延びた対称軸に対して対称となるように設置された複数の加熱部と、
前記内部筐体内の上方位置の中央部に設置され、前記内部筐体内の気体を吸い込み、吸い込んだ前記気体を排出する気体循環部と、
前記気体循環部から排出された気体を、前記対称軸に対して対称となるように、前記内部筐体内で分散させる分散部材と、
を有することを特徴とする培養装置。 - 前記分散部材は、各加熱部又は各加熱部の上方に向かって気体を吹き付けるように、前記気体循環部から排出された気体を前記内部筐体内で分散させることを特徴とする請求項1に記載の培養装置。
- 前記インキュベータ部は、前記載置部と前記加熱部との間であって、前記載置部の周縁の少なくとも一部を取り囲むようにして設置された遮熱部材をさらに有し、
前記遮熱部材の下方には、隣接する部材との間に間隙が形成されていることを特徴とする請求項1又は2のいずれかに記載の培養装置。 - 前記インキュベータ部は、前記載置部を前記対称軸を中心に回転させるための駆動部をさらに有することを特徴とする請求項1乃至3のいずれか1項に記載の培養装置。
- 前記インキュベータ部は、前記気体循環部に、調整気体を供給するための調整気体供給部をさらに有し、
前記内部筐体内の気体内の前記調整気体の濃度を調整できることを特徴とする請求項1乃至4のいずれか1項に記載の培養装置。 - 前記調整気体供給部から供給される調整気体を殺菌するための調整気体殺菌部をさらに備えたことを特徴とする請求項5に記載の培養装置。
- 前記調整気体は酸素を含み、
前記調整気体殺菌部は前記調整気体に対して紫外線を照射することを特徴とする請求項6に記載の培養装置。 - 前記インキュベータ部は、前記載置部の下方に載置され、加湿用の水を収容する加湿水収容部をさらに有することを特徴とする請求項1乃至7のいずれか1項に記載の培養装置。
- 前記インキュベータ部は、前記容器が前記載置部上に載置されているかを検知するための検知部をさらに有することを特徴とする請求項1乃至8のいずれか1項に記載の培養装置。
- 前記載置部は複数設けられており、
前記内部筐体は、各載置部に対応して設置された開閉扉を有することを特徴とする請求項1乃至9のいずれか1項に記載の培養装置。 - 前記載置部の数と前記開閉扉の数とは同数になっていることを特徴とする請求項10に記載の培養装置。
- 前記インキュベータ部は、前記対称軸に沿って上下方向で延在し、前記載置部を水平方向で保持する保持部をさらに有し、
前記載置部は、前記保持部で保持された第一載置部と、前記第一載置部の周縁外方側に位置し、前記第一載置部で保持された第二載置部と、前記第一載置部と前記第二載置部との間に載置された弾性部材と、を有することを特徴とする請求項1乃至11のいずれか1項に記載の培養装置。 - 前記載置部は複数設けられ、
前記載置部は、下方で隣接する載置部に対して上下方向で移動可能となるようにして、下方で隣接する載置部上に載置されることを特徴とする請求項1乃至12のいずれか1項に記載の培養装置。 - 前記内部筐体を取り囲むようにして配置された外部筐体と、
前記外部筐体内の上方に設置され、前記外部筐体内に気体を供給する外部気体供給部と、
前記外部筐体内の下方に設置され、前記外部気体供給部から供給された気体を排出する外部気体排出部と、をさらに備えたことを特徴とする請求項1乃至13のいずれか1項に記載の培養装置。 - 前記内部筐体を取り囲むようにして配置された外部筐体と、
前記外部筐体内に設置され、前記供給部から供給される気体を殺菌するための殺菌部と、をさらに備えたことを特徴とする請求項1乃至14のいずれか1項に記載の培養装置。 - 前記殺菌部は殺菌灯であり、
前記殺菌灯の下方には、前記殺菌灯から照射される殺菌光が前記外部筐体外に漏れ出すことを防止するための遮光板が設置されていることを特徴とする請求項15に記載の培養装置。 - 前記殺菌部は殺菌灯であり、
前記外部筐体の少なくとも一部は透明又は半透明となっており、
前記外部筐体のうち透明又は半透明となっている箇所は前記殺菌灯から照射される殺菌光が前記外部筐体外に漏れ出すことを防止する材質から形成されていることを特徴とする請求項15又は16のいずれかに記載の培養装置。 - 前記内部筐体に隣接して配置された搬送用筐体と、
前記搬送用筐体内に設置され、前記内部筐体との間で前記容器を出し入れするための搬送機構と、
前記搬送機構を上下方向に移動させる上下駆動機構と、をさらに備えたことを特徴とする請求項1乃至13のいずれか1項に記載の培養装置。 - 前記載置部は、同心円で複数の円弧状の隙間が形成された円盤状の板を有し、
円周方向において隣接する円弧状の隙間は、前記容器が載置される予定の位置で閉じて閉鎖部を形成することを特徴とする請求項1乃至18のいずれか1項に記載の培養装置。 - 前記閉鎖部の前記円周方向における距離は前記容器の前記円周方向における距離と略同一となっていることを特徴とする請求項19に記載の培養装置。
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CN116144469A (zh) * | 2023-04-18 | 2023-05-23 | 哈尔滨葵花药业有限公司 | 一种双歧杆菌培养设备 |
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DE102019134002A1 (de) * | 2019-12-11 | 2021-06-17 | Aixinno Limited | Vorrichtung zur Behandlung biologischer Zellkulturen |
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