WO2013011996A1 - 酵母菌から抽出されたポリリン酸，ポリリン酸の塩又はポリリン酸の溶媒和物を含むポリリン酸組成物及びその製造方法。 - Google Patents

酵母菌から抽出されたポリリン酸，ポリリン酸の塩又はポリリン酸の溶媒和物を含むポリリン酸組成物及びその製造方法。 Download PDF

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WO2013011996A1
WO2013011996A1 PCT/JP2012/068169 JP2012068169W WO2013011996A1 WO 2013011996 A1 WO2013011996 A1 WO 2013011996A1 JP 2012068169 W JP2012068169 W JP 2012068169W WO 2013011996 A1 WO2013011996 A1 WO 2013011996A1
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polyphosphoric acid
yeast
acid composition
cell
polyphosphoric
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PCT/JP2012/068169
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English (en)
French (fr)
Japanese (ja)
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柴　肇一
祐美 川添
加藤　真一
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リジェンティス株式会社
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Priority to CN201280035382.0A priority Critical patent/CN103687582B/zh
Priority to JP2013524728A priority patent/JP5528629B2/ja
Publication of WO2013011996A1 publication Critical patent/WO2013011996A1/ja
Priority to HK14108009.6A priority patent/HK1194662A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/24—Phosphorous; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • the present invention relates to a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid, and a method for producing the same.
  • Polyphosphoric acid is known to be easily chemically synthesized by polymerizing phosphoric acid.
  • Polyphosphoric acid is used, for example, in cosmetics, hair restorers, oral cleaning agents, food additives, medical materials and medical preparations.
  • polyphosphoric acid is produced by a chemical synthesis method such as a method of heating phosphoric acid or a method of adding and dissolving phosphorus pentoxide in phosphoric acid (Patent Document 1).
  • Non-patent Document 1 Chemically synthesized polyphosphoric acid can stabilize intracellular proteins such as cell growth factors (Non-patent Document 1). Furthermore, polyphosphoric acid has the ability to promote tissue repair, and can be used to promote healing of trauma and burns, treatment of periodontal diseases, and healing after surgery (Patent Document 2). Polyphosphoric acid is known to have an angiogenesis promoting action (Patent Document 3). Furthermore, it is known that polyphosphoric acid protects the intestines from the invasion of harmful substances and pathogenic bacteria by narrowing the gaps between the intestinal epithelial cells, and exhibits an intestinal regulating action (Non-patent Document 2).
  • a further object of the present invention is to provide a polyphosphoric acid composition that is highly safe and contains naturally synthesized polyphosphoric acid.
  • An object of the present invention is to provide a method for producing a polyphosphate composition that has high cell growth promoting ability, cell permeability, and cell life promoting ability, and further acts to promote hair elongation and maintain the hair growth phase.
  • the present inventors can produce a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid.
  • the present invention has been completed.
  • the first aspect of the present invention relates to a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid.
  • a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid has high osmotic power to cells. Furthermore, by applying the polyphosphate composition to the skin, gums, and scalp, the polyphosphate composition effectively acts on cell proliferation, cell life extension, hair elongation, and maintenance of the hair growth phase.
  • the yeast is budding yeast.
  • Budding yeast is a yeast that has been used for food since ancient times.
  • a polyphosphoric acid composition containing polyphosphoric acid, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid can be obtained efficiently.
  • budding yeast it is possible to obtain a polyphosphate composition having a high osmotic power for cells.
  • the polyphosphate composition effectively acts on cell proliferation, cell life extension, hair elongation, and maintenance of the hair growth phase.
  • the extract further contains at least one of cell constituents and protein hydrolysates of yeast.
  • a cell constituent and a protein hydrolyzate in the polyphosphate composition, nutrients derived from yeast can be contained in the polyphosphate composition.
  • the polyphosphate composition has a cell growth promoting ability, a cell permeability, and a cell life promoting ability, and more effectively acts to promote hair elongation and maintain the hair growth phase.
  • the extract further contains at least one of vitamins and minerals.
  • the polyphosphate composition has the ability to promote cell growth, permeability to cells, and the ability to promote cell life extension, and further promotes hair growth and maintains the hair growth phase. It works more effectively.
  • polyphosphoric acid, a salt of polyphosphoric acid or a solvate of polyphosphoric acid is contained in an amount of 0.01% by mass to 70% by mass with respect to the total mass of the polyphosphoric acid composition. It is what By including an effective amount of polyphosphoric acid, a salt of polyphosphoric acid or a solvate of polyphosphoric acid in the polyphosphoric acid composition, the polyphosphoric acid composition has cell proliferation promoting ability, cell permeability, and cell life promoting ability. Furthermore, it works more effectively in promoting hair elongation and maintaining the hair growth phase.
  • the degree of polymerization of polyphosphoric acid is 20 or more and 300 or less.
  • Polyphosphoric acid having a degree of polymerization of 20 or more and 300 or less has water-soluble properties and has the ability to stabilize and activate cell growth factors.
  • polyphosphoric acid has water-soluble properties.
  • the polyphosphate composition promotes the proliferation of cells of the skin and scalp and activates the cells.
  • the polyphosphoric acid has a shape with a maximum diameter of 10 nm to 1000 nm.
  • the polyphosphoric acid composition can easily penetrate into cells of the skin and scalp because the size of the polyphosphoric acid is at the nanoparticle level.
  • a preferred embodiment of the first aspect of the present invention is obtained by co-culturing yeast with lactic acid bacteria.
  • the ability of yeast to accumulate polyphosphate increases when co-cultured with lactic acid bacteria.
  • the concentration of polyphosphoric acid increases, and as a result, an excellent polyphosphoric acid composition can be obtained.
  • the second aspect of the present invention relates to cosmetics.
  • the cosmetic contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient.
  • the cosmetic functions to promote cell proliferation and cell life extension.
  • the third aspect of the present invention relates to a hair restorer.
  • the hair restorer contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient. By mixing an effective amount of the polyphosphoric acid composition with the hair restorer, the hair restorer promotes hair elongation and functions to maintain the hair growth period.
  • the fourth aspect of the present invention relates to an oral cleaning agent.
  • the oral cleaning agent contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient.
  • the oral cleaning agent effectively acts on the prevention of periodontal disease by suppressing inflammation and the regeneration of alveolar bone by the regeneration promoting effect.
  • the aspect other than the above of the present invention relates to foods, food additives, supplements or intestinal preparations. It is known that polyphosphate protects the intestine from the invasion of harmful substances and pathogenic bacteria by narrowing the gap between epithelial cells of the intestine, and exhibits intestinal regulation. Therefore, by using the polyphosphoric acid composition produced by yeast as a food, food additive or supplement, a product having an intestinal regulating action can be provided.
  • the fifth aspect of the present invention relates to a method for producing polyphosphoric acid, a salt of polyphosphoric acid or a solvate-containing product of polyphosphoric acid.
  • the method includes a step of obtaining a yeast mass, a step of obtaining a yeast culture solution, a step of obtaining a yeast precipitate, a step of obtaining a yeast suspension, a step of obtaining a yeast extract, Obtaining an acid composition.
  • the step of obtaining the yeast mass is a step of obtaining the yeast mass by allowing the yeast to stand at a temperature of 1 ° C. or higher and 10 ° C. or lower.
  • the step of obtaining a yeast culture solution is a step of culturing a yeast cell mass with a culture solution containing phosphate to obtain a culture solution containing yeast containing polyphosphoric acid.
  • the step of obtaining the yeast precipitate is a step of centrifuging the yeast culture solution to obtain the precipitate.
  • the step of obtaining the yeast suspension is a step of suspending the yeast obtained as a precipitate in a liquid and obtaining a suspension in which the yeast is suspended.
  • the step of obtaining the yeast extract is a step of obtaining a yeast extract by extracting the polyphosphoric acid from the yeast by heating the yeast suspension.
  • the step of obtaining a polyphosphate composition is a step of centrifuging a yeast extract into a supernatant containing polyphosphate and a precipitate containing a yeast cell fraction to obtain a supernatant polyphosphate composition. is there.
  • the target polyphosphoric acid composition is manufactured in the above steps.
  • the culture process of the production method according to the present invention consists of one process, and the culture time is greatly shortened.
  • the resulting polyphosphate composition has cell growth promoting ability, cell permeability, and cell life promoting ability, and more effectively acts to promote hair elongation and maintain the hair growth phase.
  • the polyphosphoric acid and the protein are heated at a temperature of 60 ° C. to 80 ° C. for 20 minutes to 40 minutes.
  • a protein hydrolyzate can be obtained by heat-treating at a temperature of 60 ° C. or higher and 80 ° C. or lower for a predetermined time or longer.
  • polyphosphoric acid composition containing protein hydrolyzate has the ability to promote cell growth, permeability to cells, and the ability to promote cell life extension, and further promote hair growth and maintain hair growth phase It works more effectively.
  • the aspect different from the above of the present invention relates to a polyphosphate-containing yeast and a supplement (food, food additive, intestinal preparation) containing such a polyphosphate-containing yeast.
  • This polyphosphate-containing yeast can be produced as follows. Specific conditions can be used by appropriately modifying the method described above. The yeast mass is cultured in a culture solution containing lactic acid bacteria and phosphate to obtain a yeast culture solution containing polyphosphoric acid. Next, the yeast culture solution is centrifuged to obtain a yeast precipitate. And the polyphosphoric acid containing yeast containing either polyphosphoric acid, the salt of polyphosphoric acid, or the solvate of polyphosphoric acid is obtained using a yeast deposit.
  • polyphosphate composition that has high cell growth promoting ability, cell permeability, and cell life promoting ability, and further acts to promote hair elongation and maintain the hair growth phase.
  • the polyphosphoric acid composition of the present invention contains polyphosphoric acid that is naturally synthesized in yeast cells, and can provide a polyphosphoric acid composition that is excellent in terms of safety.
  • the present invention it is possible to produce a polyphosphate composition having high cell growth promoting ability, cell permeability, and cell life promoting ability, and further acting to promote hair elongation and maintain the hair growth phase. it can.
  • the present invention can provide foods, food additives, supplements, or intestinal preparations that contain an effective amount of yeast or an extract thereof as an active ingredient of polyphosphoric acid that provides intestinal function by making intestinal cells healthy.
  • the production method of the present invention is excellent in that a polyphosphoric acid composition can be obtained efficiently.
  • FIG. 1 shows the molecular weight distribution of polyphosphoric acid extracted by heating from yeast.
  • FIG. 2 shows an electron micrograph of polyphosphate particles extracted from yeast.
  • FIG. 3 shows the growth promoting ability of fibroblasts by a polyphosphate composition containing polyphosphate extracted from yeast.
  • FIG. 4 is a photograph replacing a drawing showing the production of formazan in artificial skin treated with polyphosphoric acid extracted from yeast.
  • FIG. 5 shows the hair elongation promoting effect of polyphosphoric acid extracted from yeast.
  • FIG. 6 shows examples of hair follicles in the hair growth phase, regression phase, and unchanged.
  • FIG. 7 shows the effect of maintaining the hair growth stage by polyphosphoric acid extracted from yeast.
  • the first aspect of the present invention relates to a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid.
  • a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid has high osmotic power to cells. Furthermore, by applying the polyphosphate composition to the skin, gums, and scalp, the polyphosphate composition effectively acts on cell proliferation, cell life extension, hair elongation, and maintenance of the hair growth phase.
  • the polyphosphoric acid composition is a composition containing polyphosphoric acid, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid.
  • the polyphosphoric acid composition can take various forms such as a liquid, a concentrated liquid, a powder, and a solid.
  • the polyphosphoric acid composition is preferably water-soluble because it is mixed with agents such as cosmetics, hair restorers, and oral cleaning agents.
  • the polyphosphoric acid composition can further include at least one of cell constituents, protein hydrolysates, vitamins, and minerals.
  • what is contained in a polyphosphoric acid composition is not restricted to what was mentioned above, Other active ingredients can also be included.
  • Yeasts are basically eukaryotic and unicellular microorganisms.
  • Yeast has a cell wall in the outermost layer, and the cell wall is mainly composed of polysaccharides such as glucan and mannan.
  • Saccharomyces cerevisiae is a eukaryote and has a nuclear membrane and a nucleus.
  • budding yeast has cell organs such as cytoplasm, mitochondria and microtubules.
  • yeast can be broadly classified into budding yeast and fission yeast.
  • the yeast used in the present invention is not particularly limited as long as it is generally called yeast, and budding yeast and fission yeast can be appropriately used.
  • the yeast is a budding yeast.
  • Budding yeast has long been used for food processing. Budding yeast can ferment foods and break down sugars in foods into alcohols. Yeasts have long been used in the production of foods such as beer, bread, and miso, and their safety for food use has long been confirmed. Budding yeast is a yeast that proliferates by budding. Preferable examples of the budding yeast include Saccharomyces genus, Tolropsis genus, Mycotorula genus, Torlas pora genus, Candida genus, Rhodorula genus and Pichia genus.
  • a polyphosphoric acid composition containing polyphosphoric acid, a salt of polyphosphoric acid or a solvate of polyphosphoric acid can be efficiently obtained by using budding yeast. Furthermore, by using budding yeast, a polyphosphate composition that works more effectively on the skin and scalp cells can be obtained.
  • the yeast is co-cultured with lactic acid bacteria. Co-culture increases the amount of polyphosphate accumulated by the yeast being affected by lactic acid bacteria or metabolites produced by the yeast.
  • Microorganisms have the ability to take up phosphoric acid and synthesize polyphosphoric acid. Since microorganisms have an enzyme that synthesizes polyphosphate using adenosine triphosphate (ATP), polyphosphate can be synthesized from the incorporated phosphate. Since yeast is a microorganism, as described above, it has the ability to take in phosphoric acid and synthesize polyphosphoric acid.
  • ATP adenosine triphosphate
  • the yeast takes in phosphoric acid into the yeast.
  • the culture fluid is preferably pH 3 to 6, and the pH may be 3 to 8 depending on the characteristics of the target protein produced by the yeast.
  • the culture temperature is preferably 20 ° C. or higher and 35 ° C. or lower.
  • the culture solution can also contain high concentrations of minerals such as magnesium, calcium and zinc and vitamins such as vitamin B2, vitamin B6, vitamin C, pantothenic acid and folic acid. Vitamins and minerals can be contained in an amount of 0.1% to 3% by mass with respect to the total mass in the culture solution.
  • the yeast can take in minerals and vitamins into the vitamin and mineral yeast cells present in the culture solution.
  • Phosphoric acid taken into yeast cells is synthesized into polyphosphoric acid by a synthetic enzyme possessed by yeast.
  • polyphosphoric acid is stored in the yeast cells.
  • the degree of polymerization of the synthesized polyphosphate varies depending on the type of yeast and the type of polyphosphate synthase. In the case of the same type of yeast, since the synthases possessed by the yeast are the same species, the degree of polymerization of the resulting polyphosphoric acid falls within a certain range. Furthermore, by adjusting the culture temperature and culture time of the yeast and controlling the enzyme activity, polyphosphoric acid having a desired degree of polymerization can be obtained.
  • Polyphosphoric acid synthesized in yeast cells can be extracted by applying heat shock to the yeast cells and destroying the yeast cells.
  • the heating conditions are preferably 60 ° C. or higher and 80 ° C. or lower and preferably 20 minutes or longer and 40 minutes or shorter.
  • the cell wall and cell membrane of the yeast are destroyed by the heat treatment, and a yeast extract containing polyphosphoric acid, a salt of polyphosphoric acid or a solvate of polyphosphoric acid synthesized by the yeast can be obtained.
  • a part of cell constituents such as yeast cell wall, cell membrane, mitochondria, nuclear membrane, nuclear material and microtubules are also extracted into the extract.
  • the obtained yeast extract is centrifuged into a supernatant containing polyphosphoric acid and a precipitate containing the yeast cell fraction to obtain a polyphosphoric acid composition. If the size of the cell debris is greater than a certain size, the cell debris is precipitated and removed in the process of centrifuging the yeast extract. However, fine cellular constituents remain in the supernatant of the extract. Thereby, in addition to polyphosphoric acid, the polyphosphoric acid composition contains a part of yeast cell constituents. Furthermore, when the yeast cell constituents and the protein produced by the yeast are enzymatically degraded, the yeast extract contains a protein hydrolyzate. In this case, the polyphosphoric acid composition contains protein hydrolyzate in addition to polyphosphoric acid and part of the cell constituents. By including a cell constituent and a protein hydrolyzate in the polyphosphate composition, the polyphosphate composition can contain nutrients derived from yeast.
  • the culture solution contains a high concentration of minerals such as magnesium, calcium or zinc and vitamins such as vitamin B2, vitamin B6, vitamin C, pantothenic acid and folic acid, In addition to acid, it contains minerals and vitamins. Therefore, the extract extracted from the yeast by heat treatment contains vitamins and minerals contained in the yeast in addition to polyphosphoric acid.
  • yeast cell constituents, protein hydrolysates, vitamins and minerals are nutrients for skin and scalp cells and are useful for cell maintenance or growth. Therefore, when these substances are added to cosmetics, hair restorers, and oral cleaning agents, these substances act as active ingredients. Furthermore, yeast cell constituents, protein hydrolysates, vitamins, and minerals can increase the ability of polyphosphate to promote cell growth and permeability to the skin by interacting with polyphosphate.
  • the polyphosphoric acid may be linear polyphosphoric acid, side chain polyphosphoric acid, cyclic polyphosphoric acid, or polyphosphoric acid (ultraphosphoric acid), which is a highly branched (network-like) phosphoric acid polymer. It may be a mixture or a derivative thereof.
  • the linear polyphosphoric acid has a structure in which two or more PO 4 tetrahedrons share an apex oxygen atom and are linearly connected by dehydration condensation of orthophosphoric acid.
  • Side-chain polyphosphoric acid has a structure in which an organic group is introduced into a direct side chain.
  • the polyphosphoric acid is a linear or cyclic compound represented by H n + 2 (P n O 3n + 1 ) or (HPO 3 ) n with H 2 O and P 2 O 5 as constituent molecules.
  • the number of polymerized phosphoric acids is represented by n.
  • the polyphosphoric acid mainly includes polyphosphoric acid having a polymerization degree of 20 to 100 (medium-chain polyphosphoric acid) and polyphosphoric acid having a polymerization degree of 100 to 1000 (long-chain polyphosphoric acid).
  • the degree of polymerization of polyphosphoric acid is 20 to 300, preferably 50 to 250, more preferably 80 to 200.
  • Polyphosphoric acid having a degree of polymerization of 20 or more and 300 or less is water-soluble, and has the ability to promote cell growth by stabilizing cell growth factors such as fibroblast growth factor.
  • the polyphosphoric acid composition of the present invention includes polyphosphoric acid, a salt of polyphosphoric acid, or a solvate of polyphosphoric acid.
  • the salt of polyphosphoric acid means a salt of polyphosphoric acid, particularly a pharmaceutically acceptable salt of polyphosphoric acid.
  • a salt of polyphosphoric acid an alkali metal salt is preferable, and a sodium salt is more preferable.
  • the salt of polyphosphoric acid may contain not only anhydrous salts but also hydrated salts. These salts, for example, ionize in solution and function in the same way as polyphosphoric acid.
  • the solvate of polyphosphoric acid means a solvate of a solvate of polyphosphoric acid.
  • solvates include hydrates.
  • the polyphosphoric acid composition of this invention may become a hydrate by absorbing water and attaching adsorbed water in yeast cells or in an extract. Such a solvate is also included in the solvate of polyphosphoric acid. These solvates function like polyphosphoric acid by ionizing in solution.
  • the polyphosphoric acid, polyphosphoric acid salt or polyphosphoric acid solvate used in the present invention may be one kind or a mixture of plural kinds.
  • Multiple types of polyphosphoric acid, polyphosphoric acid salt or solvate of polyphosphoric acid include polyphosphoric acid having a different degree of polymerization or molecular structure, polyphosphoric acid salt or solvate of polyphosphoric acid, and polyphosphoric acid having different metal ions Contains salt.
  • the polyphosphoric acid is 0.01% by mass or more and 70% by mass or less, preferably 0.02% by mass or more and 50% by mass or less, and more preferably 0.1% by mass or more based on the total mass of the polyphosphoric acid composition. It is preferable that it is contained in an amount of 50% by mass or more.
  • the polyphosphoric acid may be a salt of polyphosphoric acid or a solvate of polyphosphoric acid, and the content of polyphosphoric acid in the polyphosphoric acid composition is the content of the total mass of these three types of polyphosphoric acid.
  • polyphosphoric acid By containing an effective amount of polyphosphoric acid, polyphosphoric acid salt or solvate of polyphosphoric acid in the polyphosphoric acid composition, it is effective for promoting cell proliferation, prolonging the life of skin cells, maintaining hair growth and hair growth phase, and regulating the bowel Act on.
  • the polyphosphoric acid obtained in the present invention is synthesized in the yeast cells and stored in the yeast cells. Because of the stable presence of polyphosphate in yeast cells, polyphosphate exists in a folded structure.
  • the polyphosphoric acid in a folded state has a maximum diameter of 10 nm to 1000 nm, preferably 30 nm to 800 nm, and more preferably 50 nm to 500 nm. Since the polyphosphoric acid obtained by the present invention has a nano-order level particle size, it has excellent permeability to the skin surface. Moreover, polyphosphoric acid becomes easy to adapt to the layer of the fats and oils component on a scalp and skin by becoming the said shape, and permeability improves.
  • the folded structure means a particle shape in which polyphosphoric acid is aggregated, and the shape includes various shapes such as a spherical shape, an elliptical shape, a cylindrical shape, a quadrangular shape, and an uneven lump shape.
  • the second aspect of the present invention relates to cosmetics.
  • the cosmetic contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient.
  • the cosmetic according to the present invention contains a polyphosphoric acid composition as an active ingredient.
  • a polyphosphoric acid composition is added to cosmetics in the state of a liquid, a concentrate, a powder, and a solid substance.
  • the polyphosphoric acid is 0.01% by mass or more and 70% by mass or less, preferably 0.02% by mass or more and 50% by mass or less, more preferably 0.1% by mass or more and 50% by mass or less with respect to the total mass of the cosmetic amount. The following are preferably included.
  • ком ⁇ онентs can be further added to the cosmetic according to the present invention.
  • purified water for supplying water to the stratum corneum such as ion-exchange water, and oil, glycerin, PEG, and hyaluron for dissolving oil-soluble components such as ethanol and propanol to give a refreshing feeling
  • Moisturizer for moisturizing the stratum corneum such as acid, emollient (oil component that prevents moisture from evaporating) such as ester oil and vegetable oil, and ingredients such as polyoxyethylene oleyl alcohol ether Solubilizers to solubilize, buffers to adjust the pH of products such as citric acid, lactic acid, and amino acids, fragrances such as vanillin, orange flavor, lemon flavor, milk flavor, strawberry geraniol, and linalool Perfume, methylparaben, and phenoxyethanol Anti-fading agent, astringent, disinfectant to prevent discoloration and discoloration, such as anti
  • Cosmetics are generally applied to the skin surface in order to keep the skin clean and healthy.
  • Examples of cosmetics include lotion, milky lotion, cream, gel, cosmetic liquid (essence), and pack cosmetics.
  • a cosmetic containing a polyphosphoric acid composition as an active ingredient can be produced according to a usual method.
  • the basic function of lotion is to supply moisture and moisturizing ingredients to the stratum corneum of the skin, and lotion also has the function of making the skin soft.
  • the active ingredient of skin lotion is required to penetrate into the stratum corneum, and those having a high ability to penetrate the skin are preferred.
  • polyphosphoric acid synthesized in yeast has a nano-order particle size. Therefore, polyphosphoric acid synthesized in yeast cells has a high ability to penetrate the skin, has the ability to promote cell growth, and works to increase the survival effect of skin cells. This can keep skin cells healthy.
  • the third aspect of the present invention relates to a hair restorer.
  • the hair restorer contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient.
  • the hair restorer according to the present invention contains a polyphosphoric acid composition as an active ingredient.
  • a polyphosphoric acid composition is added to a hair restorer in the state of a liquid, a concentrate, a powder, and a solid substance.
  • the polyphosphoric acid is 0.01% by mass or more and 70% by mass or less, preferably 0.02% by mass or more and 50% by mass or less, and more preferably 0.1% by mass or more and 50% by mass or more based on the total mass of the hair restorer. The following are preferably included.
  • the hair restorer may be administered in an amount of 0.01 g to 10 g (preferably 0.1 g to 5 g) in the range of 1 to 10 times a day in terms of the weight of the polyphosphoric acid composition.
  • hair-growth ingredients can be further added to the hair-growth agent according to the present invention.
  • biological cell activators such as laurel extract and chlorophyll, ginseng extract, assembly extract, cephalanthin, vitamin E and its derivatives, and blood circulation promoters such as gamma oryzanol that promote blood flow in peripheral blood vessels, red pepper tincture, ginger tincture, Cantharis tincture and topical stimulants such as nicotinic acid benzyl ester, vitamins A, B1, B2, B6, E and their derivatives, pantothenic acid and its derivatives, vitamins such as biotin, cystine, cysteine, methionine, leucine, tryptophan , And an amino acid extract such as a nutritional supplement for feeding around hair matrix cells, a female hormone having an antagonistic effect on male hormones such as estradiol and ethinyl estradiol, pan Hair root activator for improvement of hair cell function deterioration such as tennoic acid and its derivatives
  • Hair growth agents are generally effective in promoting hair growth, hair growth, hair growth, hair restoration and hair loss prevention.
  • the hair restorer is generally applied directly to the scalp.
  • hair restorers include solutions, emulsions, creams, lotions, gels, foams, and sprays.
  • a polyphosphoric acid composition may be included as an active ingredient in shampoos, rinses and treatments.
  • the poultice which impregnated the active ingredient into the nonwoven fabric or cotton may be used.
  • a hair restorer containing a polyphosphoric acid composition as an active ingredient can be produced according to a usual method.
  • the basic function of hair restorer is to maintain the activation of hair follicle cells in the scalp and to elongate hair.
  • the hair-growth agent preferably has a high ability to penetrate hair follicle cells in order to penetrate the active ingredient into the hair follicle cells of the scalp.
  • polyphosphoric acid synthesized in yeast has a nano-order particle size. Therefore, polyphosphoric acid synthesized in yeast cells has a high ability to penetrate hair follicle cells and can sustain the activation of hair follicle cells. Therefore, it is possible to promote hair elongation and to maintain the hair follicle cells in a growth phase.
  • the fourth aspect of the present invention relates to an oral cleaning agent.
  • the oral cleaning agent contains an effective amount of a polyphosphoric acid composition extracted from yeast as an active ingredient.
  • the oral cleaning agent according to the present invention contains a polyphosphoric acid composition as an active ingredient.
  • a polyphosphoric acid composition is added to an oral cleaning agent in the state of a liquid, a gel, a paste, a concentrated liquid, a powder, and a solid.
  • the polyphosphoric acid is 0.01% by mass or more and 70% by mass or less, preferably 0.02% by mass or more and 50% by mass or less, and more preferably 0.1% by mass or more and 50% by mass or less with respect to the total mass of the oral cleaning agent. % Or less is preferable.
  • the oral cleaning agent may be administered in an amount of 0.01 g or more and 10 g or less (preferably 0.1 g or more and 5 g or less) in a range of once to 10 times a day in terms of the weight of the polyphosphoric acid composition.
  • oral cleaning ingredients can be added to the oral cleaning agent according to the present invention.
  • cleaning agents such as polyethylene glycol, polyvinylpyrrolidone, phytic acid, sodium hydrogen phosphate, sodium phosphate, and zeolite
  • bleaching agents such as hydrogen peroxide, urea peroxide, calcium peroxide and sodium chlorite, allantoin
  • ⁇ -Anti-inflammatory agents such as aminocaproic acid, dipotassium glycyrrhizinate, ⁇ -glycyrrhetinic acid, ascorbic acid, vitamin E, and sodium azulenesulfonate, isopropylmethylphenol, chlorhexidine hydrochloride, triclosan, hinokitiol, cetylpyridinium chloride, copper chlorophyllin sodium, benzal chloride
  • Antibacterial agents such as luconium, depotassium chloride, benzethonium chloride, alkyldiaminoethylgly
  • Oral cleaning agents generally stay in the oral cavity for a certain period of time, purify the oral cavity, prevent drying, give a refreshing feeling, or have an effect of preventing or treating oral diseases.
  • oral cleaning agents include toothpaste, abrasives, mouth washes, spray-type oral cleaning agents, gargles, and oral rinses.
  • An oral cleaning agent containing a polyphosphoric acid composition as an active ingredient can be produced according to a usual method.
  • the basic function of the oral cleaning agent is to promote oral cleaning and periodontal tissue including alveolar bone and dental health. It is preferable that the oral cleaning agent has a high penetration ability into the periodontal pocket in order to allow the active ingredient to penetrate into the cells of the periodontal tissue and suppress inflammation of the gums.
  • polyphosphoric acid synthesized in yeast has a nano-order particle size. Therefore, the polyphosphate composition synthesized in yeast has a high ability to penetrate periodontal tissues into gum cells and alveolar bone, promotes the growth of gum cells and suppresses inflammation and promotes alveolar bone regeneration be able to.
  • a preferred aspect of the present invention relates to a food containing an effective amount of any of the polyphosphoric acid compositions described above as an active ingredient.
  • the kind of food is not particularly limited. If the food exhibits any of the effects described above, the polyphosphoric acid composition in an amount capable of exhibiting the effects may be included.
  • An example of food is a beverage. If it is a drink, the polyphosphoric acid composition of this invention can be ingested easily. Examples of beverages are soft drinks, tea, coffee, tea and lactic acid drinks. What is necessary is just to administer 0.01 g or more and 10 g or less (preferably 0.1 g or more and 5 g or less) of the food to the subject within a range of 1 to 10 times a day in terms of the weight of the polyphosphoric acid composition.
  • a preferred aspect of the present invention relates to a food additive containing an effective amount of any of the polyphosphoric acid compositions described above as an active ingredient.
  • the food additive of this embodiment is appropriately added to foods in order to exhibit the above-described effects of the polyphosphoric acid composition.
  • a preferred aspect of the present invention relates to a supplement containing an effective amount of any of the polyphosphoric acid compositions described above as an active ingredient.
  • Supplements themselves are known. That is, a supplement can be produced by mixing the polyphosphoric acid composition of the present invention with a known carrier (starch or the like) and molding with a tableting machine.
  • the food, food additive or supplement according to the present invention includes a polyphosphoric acid composition.
  • the polyphosphoric acid is 0.01% by mass or more and 50% by mass or less, preferably 0.02% by mass or more and 30% by mass or less, more preferably 0.1% by mass or more and 15% by mass or less, based on the total mass of the supplement. included.
  • Supplements include sugars such as lactose and oligosaccharides, excipients such as dextrin and cellulose, various inorganic salts, vitamins, collagen, hyaluronic acid, coenzyme Q10, ⁇ lipoic acid, various plant extracts and other useful ingredients, flavors, Sweeteners and the like may be included as appropriate.
  • the supplement may be administered to the subject in a range of 0.01 to 10 g (preferably 0.1 to 5 g) in the range of 1 to 10 times a day in terms of the weight of the polyphosphoric acid composition.
  • a preferred aspect of the present invention relates to an intestinal regulating agent containing an effective amount of any of the polyphosphoric acid compositions described above as an active ingredient.
  • An intestinal regulating agent can be produced in the same manner as the above supplement. What is necessary is just to administer 0.01 g or more and 10 g or less (preferably 0.1 g or more and 5 g or less) of the intestinal regulating agent in the range of once to 10 times a day in terms of the weight of the polyphosphoric acid composition.
  • Another aspect of the present invention relates to a method for producing polyphosphoric acid, a salt of polyphosphoric acid, or a solvate-containing product of polyphosphoric acid.
  • the method includes a step of obtaining a yeast mass, a step of obtaining a yeast culture solution, a step of obtaining a yeast precipitate, a step of obtaining a yeast suspension, a step of obtaining a yeast extract, Obtaining an acid composition.
  • the step of obtaining the yeast mass is a step of obtaining the yeast mass by allowing the yeast to stand at a temperature of 1 ° C. or higher and 10 ° C. or lower.
  • the step of obtaining a yeast culture solution is a step of culturing a yeast cell mass with a culture solution containing phosphate to obtain a culture solution containing yeast containing polyphosphoric acid.
  • the step of obtaining the yeast precipitate is a step of centrifuging the yeast culture solution to obtain the precipitate.
  • the step of obtaining the yeast suspension is a step of suspending the yeast obtained as a precipitate in a liquid and obtaining a suspension in which the yeast is suspended.
  • the step of obtaining the yeast extract is a step of obtaining a yeast extract by extracting the polyphosphoric acid from the yeast by heating the yeast suspension.
  • the step of obtaining a polyphosphate composition is a step of centrifuging a yeast extract into a supernatant containing polyphosphate and a precipitate containing a yeast cell fraction to obtain a supernatant polyphosphate composition. is there.
  • Yeast can take up phosphoric acid into the yeast and synthesize polyphosphoric acid in the fungus. By culturing the yeast in a culture solution containing phosphate, the yeast takes in phosphoric acid.
  • a method for incorporating phosphoric acid into yeast cells and synthesizing polyphosphoric acid a method of culturing two steps is generally known.
  • the first step is a step for cultivating the yeast in a culture solution that does not contain any phosphate to make the yeast enter a phosphate-starved state so that the yeast can easily take in phosphate.
  • the second step is a step of culturing the yeast in a culture solution rich in phosphoric acid and incorporating the phosphoric acid into the yeast.
  • the yeast can efficiently incorporate phosphoric acid into the cells by setting the culture process to two steps.
  • the cells cultured with the first type of culture solution must be collected and cultured again with a new culture solution. Therefore, the conventional culturing process has problems in terms of operation and cost.
  • the step of obtaining the yeast cell mass is a step of allowing the yeast to stand at 1 ° C. or more and 10 ° C. or less to obtain the yeast cell mass.
  • the yeast is allowed to stand at a moisture content condition of 30% by mass to 50% by mass with respect to the yeast mass.
  • the yeast is preferably allowed to stand at 1 ° C. or higher and 10 ° C. or lower, preferably 72 hours or longer.
  • no medium or culture solution is required, and it is not necessary to exchange the culture solution after obtaining the yeast cell mass. Therefore, the culture process procedure is simplified. Furthermore, through the step of obtaining the yeast cell mass, the culture time in the step of obtaining the yeast culture solution is greatly shortened.
  • the step of obtaining the yeast culture solution is a step of culturing the yeast cell mass in a culture solution containing phosphate.
  • the culture solution can contain high concentrations of minerals such as magnesium, calcium and zinc, and vitamins such as vitamin B2, vitamin B6, vitamin C, pantothenic acid and folic acid.
  • the process of obtaining the yeast culture solution consists of only one process. In the production method according to the present invention, about 1.5 times as much polyphosphoric acid can be obtained as compared to the production process including the conventional two-step culture process.
  • the culturing time is 1 hour or more and 3 hours or less, which is significantly shortened as compared with the culturing time required in the conventional two-step culturing process. Furthermore, by culturing yeast in the presence of minerals and vitamins, not only can a polyphosphoric acid composition suitable for cosmetics, hair restorers and oral cleaning agents be obtained, but also the culture time should be shortened. You can also.
  • the culture solution preferably contains lactic acid bacteria.
  • Lactic acid bacteria are preferably added in an amount of 0.1% to 50% by weight of the yeast, and 0.5% to 20% by weight, 1% to 15% by weight, 2% to 10%. The mass% or less may be sufficient.
  • Examples of lactic acid bacteria are Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus delbrucchi, Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus kefir, Lactobacillus paracasei, Lactobacillus ⁇ Plantalum, Lactobacillus rhamnosus, Lactobacillus salivalius, Streptococcus thermophilus, Lactococcus lactis, Lactococcus plantarum, Lactococcus raffinolactis, Leuconostoc lactis, Leuconostoc mecenteroides Or it is 2 or more types. Lactic acid bacteria may be added to
  • the step of obtaining the yeast precipitate is a step of centrifuging the liquid in which the yeast is cultured to obtain the precipitated yeast. Centrifugation is performed at a centrifugal force of 3000 ⁇ g to 15000 ⁇ g for 3 minutes to 30 minutes. Centrifugation can be performed by a conventional method.
  • the step of obtaining a yeast suspension is a step of suspending a yeast precipitate obtained by centrifugation with a cell suspension solvent.
  • a cell suspension solvent a solution usually used for cell suspension such as distilled water, physiological saline, phosphate buffer, and pH buffer can be used.
  • the step of obtaining the yeast extract is a step of applying heat to the yeast extract.
  • the heat treatment can be performed at a temperature of 60 to 80 ° C. for 20 to 40 minutes. It is preferable to set the temperature and time conditions for the heat treatment so that the synthesized polyphosphoric acid is not decomposed.
  • the step of obtaining a polyphosphoric acid composition is a step of centrifuging a yeast extract into a supernatant containing polyphosphoric acid and a yeast cell fraction to obtain a polyphosphoric acid composition.
  • Polyphosphoric acid eluted from the cells is water-soluble and dissolves in the liquid. Therefore, it can isolate
  • yeast extract contains yeast cell constituents, protein hydrolysates of proteins, vitamins and minerals.
  • yeast cell constituents, protein hydrolysates, vitamins and minerals are useful for the maintenance or growth of skin and scalp cells and act as active ingredients in cosmetics, hair restorers and oral cleaners. Therefore, it is preferable to leave these substances in the supernatant in addition to polyphosphoric acid during the centrifugation in the step of obtaining the polyphosphoric acid composition. Therefore, it is preferable that the centrifugal separation is performed with a relatively small centrifugal force such as 5000 ⁇ g to 10,000 ⁇ g, which is possible with a normal centrifugal separator.
  • the step of obtaining the yeast extract is a step of performing a heat treatment at a temperature of 60 ° C. to 80 ° C. for 20 minutes to 40 minutes.
  • the step of obtaining the yeast extract in the method for producing the polyphosphoric acid composition described above is performed at a temperature of 60 ° C. or higher and 80 ° C. or lower, preferably 65 ° C. or higher and 75 ° C. or lower, 20 minutes or longer and 40 minutes or shorter, preferably 25 minutes or longer.
  • This is a step of heat treatment for 35 minutes or less. Since the synthesized polyphosphoric acid may be decomposed depending on the heating temperature and heating time conditions, the heat treatment time is preferably 40 minutes or less.
  • a protein hydrolyzate can be obtained in the step of obtaining the yeast extract.
  • a protein hydrolyzate can be obtained by hydrolyzing proteins in yeast cells. Therefore, it is preferable to heat-treat at a temperature of 80 ° C. or lower for a predetermined time or longer so that the hydrolyzate enzyme in the yeast is not inactivated.
  • the production process includes a step of heat treatment at a temperature of 60 ° C. to 80 ° C. for 20 minutes to 40 minutes, denaturation of heat-sensitive proteins and vitamins Is suppressed.
  • polyphosphoric acid nanoparticles encapsulate and protect proteins and vitamins when extracting proteins and vitamins together with polyphosphoric acid from yeast.
  • Polyphosphoric acid extracted from yeast also has a function to stabilize heat-labile components.
  • the aspect different from the above of the present invention relates to a polyphosphate-containing yeast and a supplement (food, food additive, intestinal preparation) containing such a polyphosphate-containing yeast.
  • This polyphosphate-containing yeast can be produced as follows. Specific conditions can be used by appropriately modifying the method described above. The yeast mass is cultured in a culture solution containing lactic acid bacteria and phosphate to obtain a yeast culture solution containing polyphosphoric acid. Next, the yeast culture solution is centrifuged to obtain a yeast precipitate. And the polyphosphoric acid containing yeast containing either polyphosphoric acid, the salt of polyphosphoric acid, or the solvate of polyphosphoric acid is obtained using a yeast deposit. At this time, treatment such as heating may be performed to kill the yeast (and lactic acid bacteria). If the polyphosphate-containing yeast contains dead bodies of yeast or lactic acid bacteria, they become the food for intestinal bacteria, so that a more preferable effect is exhibited, and the production of supplements and the like is facilitated.
  • the yeast used for culture was raw food grade yeast (Oriental East Regular, Oriental Yeast).
  • the yeast was stored at 10 ° C. or lower for 3 days to obtain a yeast cell mass.
  • the yeast was cultured only with the culture solution B having the composition described in Table 1.
  • the culture time and the amount of yeast cells are as shown in Table 2.
  • the amount of yeast cells is shown as a ratio of the amount of cells to the amount of the culture solution. 40 mL of the culture solution B was prepared, and yeast was added thereto so that the ratio shown in Table 2 was obtained.
  • the culture solution containing the yeast was cultured with shaking at 25 ° C. for 2 hours. After shaking culture, the culture was centrifuged at 4000 ⁇ g for 1 minute to recover the yeast cell precipitate.
  • the collected cells were cleaned once with distilled water and stored at ⁇ 30 ° C.
  • [Comparative Example 1] In order to bring the yeast into a phosphate-starved state, the culture was performed in a culture solution A containing no phosphate. The culture time and the amount of yeast cells are as shown in Table 2. The amount of yeast cells is shown as a ratio of the amount of cells to the amount of each culture solution. 300 mL of culture solution A was prepared, and 6 g of yeast was added thereto. Thereafter, the culture solution containing yeast was cultured at 25 ° C. with shaking for each time described in Table 2. After shaking culture, the culture was centrifuged at 4000 ⁇ g for 1 minute to recover the yeast cell precipitate. Next, 40 mL of the culture solution B was prepared, and the yeast cells collected therein were added so as to have the ratio shown in Table 2.
  • the culture solution containing the yeast was cultured with shaking at 25 ° C. for 2 hours. After shaking culture, the culture was centrifuged at 4000 ⁇ g for 1 minute to recover the yeast cell precipitate. The collected cells were cleaned once with distilled water and stored at ⁇ 30 ° C.
  • the culture media A and B were both adjusted to pH 5.2 with succinate buffer or phosphate buffer.
  • Example 1-5, 1-6, and 1-7 in which the culture solution A was not used it was found that the accumulated amount was as large as 0.10 to 0.22 mmol.
  • the polyphosphoric acid content was the highest, 1.5 times or more that of Comparative Example 1-3.
  • the culture time was as short as 2 hours. Furthermore, there was no need to change the culture medium.
  • the manufacturing method of the polyphosphoric acid composition containing the polyphosphoric acid extracted from a yeast it turned out that the manufacturing method of Example 1 is excellent in operativity and the cost side compared with the manufacturing method of the comparative example 1.
  • the conditions for extracting polyphosphate from yeast were examined.
  • the heat treatment was performed such that the temperature (60 to 0 ° C.) and the time (0 to 60 minutes) of the heat treatment were in the conditions shown in Table 4, and the extraction rates of polyphosphoric acid were compared.
  • the yeast culture was centrifuged at 4000 ⁇ g for 1 minute to recover the yeast cell precipitate.
  • the recovered yeast cells were frozen at -20 ° C. Thereafter, the cells were thawed and resuspended in distilled water, and 50 ⁇ L each was dispensed into a microtube.
  • Nitric acid was added to the extract obtained by the above method so that the final concentration was 3.4%, and the polyphosphoric acid was completely hydrolyzed by heat treatment at a temperature of 100 ° C. for 30 minutes. Subsequently, vanadic acid / molybdic acid test solution was added, and the amount of phosphoric acid was quantified. The amount of polyphosphoric acid was calculated from the quantified amount of phosphoric acid. The calculated results are shown in Table 4. From the results in Table 4, the highest polyphosphoric acid extraction amount was obtained when heated at 80 ° C. for 30 minutes.
  • a polyphosphoric acid composition containing polyphosphoric acid extracted from yeast was lyophilized to obtain a dried powder of polyphosphoric acid extract.
  • the fine structure of the dry powder was observed with a scanning electron microscope. As shown in FIG. 2, a large number of particles having a diameter of 1000 nanometers or less were confirmed together with a structure in which the components of the yeast cell were considered to be dried. From this, it was confirmed that the polyphosphoric acid extracted from yeast exists as particles having a nano-order diameter.
  • the obtained extract P was sterilized by filtration with a 0.45 ⁇ m sterilization filter, and the protein concentration and the amount of phosphoric acid were measured.
  • the concentration of polyphosphoric acid in the extract P was 91.1 mM.
  • the protein concentration contained in the extract P was 15.89 mg / ml.
  • the cell culture medium was replaced with a D-MEM medium supplemented with each extract containing polyphosphoric acid, and MTS reaction was performed 24, 48, and 69 hours after, respectively, and OD 492 nm was measured on a plate reader 95 minutes after the start of the reaction. Measured with Each extract was prepared so that the protein concentration was 0.174 mg / ml. In this case, the polyphosphate concentration of the extract P was 1 mM in the medium. The polyphosphate concentration of Extract Q was 0.0115 mM in the medium. The polyphosphoric acid concentration of the medium to which the extract P is added is about 87 times the polyphosphoric acid concentration of the medium to which the extract Q is added. Moreover, what was added so that the polyphosphoric acid density
  • Example 4-1 had the highest growth rate, and it was found that the extract containing 1 mM polyphosphoric acid extracted from yeast had a very high cell growth promoting activity. It was also found that the extract containing 0.2 mM polyphosphoric acid extracted from the yeast of Example 4-2 also had a relatively high growth promoting activity. It was found that the growth promoting ability depends on the content of polyphosphate. In Comparative Example 2-1, which hardly contained polyphosphoric acid, the growth promoting ability was lower than that containing polyphosphoric acid.
  • the quantification method was performed by quantifying the amount of phosphoric acid using a vanadic acid / molybdic acid test solution, and calculating the amount of polyphosphoric acid from the value.
  • Table 6 shows the results of comparing the permeability to artificial skin. From the results of Table 6, it was confirmed that in artificial skin to which polyphosphoric acid extracted from yeast was added, the polyphosphoric acid extracted from yeast penetrated about 2.1 times the amount of medium-chemically synthesized polyphosphoric acid. Therefore, it was found that polyphosphoric acid extracted from yeast penetrates the skin more easily than chemically synthesized polyphosphoric acid.
  • MTT [3- (4,5-dimethylthiazol-2-yl) -diphenyltetrazole bromide] is dissolved in TBS to 0.333 mg / ml, and 1.2 ml of TBS in which MTT is dissolved in the lower layer solution of artificial skin. After incubating at 37 ° C. for 4 hours, formation of insoluble formazan was confirmed. The result is shown in FIG. Furthermore, artificial skin was then collected, and formazan precipitated in the artificial skin was dissolved in 0.3 ml of isopropanol, and the absorbance at 595 nm was measured. The results are shown in Table 7.
  • FIG. 4 is a photograph replacing a drawing which shows the amount of formazan deposited from the mitochondria reducing ability of living cells by the MTT method.
  • the formation of formazan was confirmed with the naked eye on artificial skin cultured by adding polyphosphoric acid extracted from 20 mM or 100 mM yeast.
  • the production of formazan was remarkably confirmed in those treated with 100 mM polyphosphoric acid extracted from yeast. From the results in Table 7, the amount of formazan produced was 0.1499 treated with 20 mM polyphosphoric acid extracted from yeast.
  • the amount of formazan produced was 0.8089 treated with 100 mM polyphosphoric acid extracted from yeast. From this, it was found that the cells treated with 100 mM polyphosphoric acid extracted from the mother bacteria had high cell viability.
  • a single hair follicle tissue is isolated from human scalp tissue collected at the time of hair transplantation, and the isolated hair follicles are treated with medium-long chain polyphosphate (5 mM) having an average chain length of 60 or more and intracellular polyphosphate (5 mM) derived from yeast. Culturing was carried out for 12 days in the culture medium. The length of the hair was measured over time during the culture, and the total length of the hair is shown in FIG. From the results of FIG.
  • hair follicles cultured in a culture solution containing internal polyphosphate extracted from yeast derived from yeast have a higher degree of hair elongation than hair follicles cultured in a culture solution containing medium-long chain polyphosphate. I understood it.
  • the hair follicles were used to evaluate the ratio of hair follicles at the growing stage.
  • a single hair follicle tissue was isolated in the same manner as in Example 7, and the isolated hair follicle was cultured in a culture solution containing chemically synthesized polyphosphate (5 mM) and polyphosphate (5 mM) extracted from yeast. Cultured for days. After that, as shown in FIG.
  • FIG. 6 shows the evaluation of the hair follicle elongation phase, regression phase, and unchanged phase. The resulting ratio is shown in FIG.
  • the hair follicles treated with polyphosphoric acid extracted from yeast had an elongation period of 87% up to the 4th day of culture and 62% in the 6th day.
  • the hair follicles treated with synthetic polyphosphate 62% is in the extension phase on the second day, 62% on the fourth day, 50% on the sixth day, and the ratio of the hair follicles in the extension phase increases with time. Declined. Therefore, it was found that the hair follicles treated with polyphosphoric acid extracted from the yeast had a long elongation period and the hair follicles were activated.
  • Lactobacillus Casei was mixed with yeast (Oriental yeast regular, Oriental yeast) at a ratio of 2.5% (Example 9-1) or 5% (Example 9-2) of the weight of the yeast, Polyphosphoric acid accumulation treatment was performed by the method of Example 1-5.
  • yeast Oriental yeast regular, Oriental yeast
  • Example 9-1 a preparation containing polyphosphoric acid only in yeast
  • Example 9-2 a substance containing polyphosphoric acid accumulated only in lactic acid bacteria were prepared, and the amount of polyphosphoric acid produced by extracting polyphosphoric acid from them was determined. Compared. The results are shown in Table 8.
  • Example 9-2 Compared to Comparative Example 9-1, the amount of polyphosphoric acid accumulated increased to 106.3% in Example 9-1 and 121.7% in Example 9-2, in which lactic acid bacteria were mixed and cultured. In the case of lactic acid bacteria alone (Comparative Example 9-2), the accumulation of polyphosphoric acid was below the measurement limit value, so it is considered that the addition of lactic acid bacteria increased the ability of the yeast to accumulate polyphosphate. Therefore, when lactic acid bacteria are mixed with yeast and cultured, the ability to produce polyphosphate can be increased.

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JP2015065858A (ja) * 2013-09-27 2015-04-13 Ｍｃフードスペシャリティーズ株式会社 酵母エキス
JP2015203023A (ja) * 2014-04-15 2015-11-16 ライオン株式会社 歯磨組成物
JP2015218151A (ja) * 2014-05-20 2015-12-07 ライオン株式会社 歯磨組成物
JP2016023160A (ja) * 2014-07-18 2016-02-08 ライオン株式会社 歯磨組成物
KR101817917B1 (ko) * 2016-01-26 2018-02-22 김보람 금은화 추출물 및 효모 추출물을 포함하는 미스트액 제조 방법
WO2021099636A1 (de) 2019-11-22 2021-05-27 Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen Zusammensetzung, enthaltend getrocknetes polyphosphat und verfahren zur gewinnung von polyphosphat aus polyphosphat-haltigen hefezellen dazu

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CN113730436B (zh) * 2021-09-27 2023-10-13 北京普世安生物科技有限公司 一种具有调控毛发生长的多聚磷酸盐及其制备方法

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JP2016023160A (ja) * 2014-07-18 2016-02-08 ライオン株式会社 歯磨組成物
KR101817917B1 (ko) * 2016-01-26 2018-02-22 김보람 금은화 추출물 및 효모 추출물을 포함하는 미스트액 제조 방법
WO2021099636A1 (de) 2019-11-22 2021-05-27 Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen Zusammensetzung, enthaltend getrocknetes polyphosphat und verfahren zur gewinnung von polyphosphat aus polyphosphat-haltigen hefezellen dazu

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