WO2012029075A1 - Suppléments nutritionnels issus de légumes verts à feuilles - Google Patents

Suppléments nutritionnels issus de légumes verts à feuilles Download PDF

Info

Publication number
WO2012029075A1
WO2012029075A1 PCT/IN2011/000591 IN2011000591W WO2012029075A1 WO 2012029075 A1 WO2012029075 A1 WO 2012029075A1 IN 2011000591 W IN2011000591 W IN 2011000591W WO 2012029075 A1 WO2012029075 A1 WO 2012029075A1
Authority
WO
WIPO (PCT)
Prior art keywords
fraction
composition
lpc
juice
curd
Prior art date
Application number
PCT/IN2011/000591
Other languages
English (en)
Other versions
WO2012029075A4 (fr
Inventor
Chitra Vasant Savangikar
Vasant Anantrao Savangikar
Original Assignee
Chitra Vasant Savangikar
Vasant Anantrao Savangikar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chitra Vasant Savangikar, Vasant Anantrao Savangikar filed Critical Chitra Vasant Savangikar
Priority to US13/819,903 priority Critical patent/US9993018B2/en
Priority to EP11767305.3A priority patent/EP2611311A1/fr
Publication of WO2012029075A1 publication Critical patent/WO2012029075A1/fr
Publication of WO2012029075A4 publication Critical patent/WO2012029075A4/fr
Priority to US15/990,534 priority patent/US20180271133A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/05—Treating milk before coagulation; Separating whey from curd
    • A23C19/054—Treating milk before coagulation; Separating whey from curd using additives other than acidifying agents, NaCl, CaCl2, dairy products, proteins, fats, enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/133—Fruit or vegetables
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00—Soups; Sauces; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/14—Dried spices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/101—Addition of antibiotics, vitamins, amino-acids, or minerals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/161—Puffed cereals, e.g. popcorn or puffed rice
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/40—Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2240/00—Use or particular additives or ingredients
    • A23C2240/15—Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • Invention pertains to preparing nutritional supplements from green leafy vegetation. More particularly it pertains to preparing easily consumable or palatable synergistic compositions of a nutritional supplements from green leafy vegetables.
  • a person shall remain in a healthy state if he consumes all the essential nutrients every day through food.
  • the nutrients considered as "Essential" are those that are not synthesized in human body and must be consumed through food. They, however, come from diverse food sources, are most of the times associated with other desired or undesired constituents such as water and most of such sources are perishable too. Availability of such food sources may also fluctuate with seasons, market conditions and location. Under practical circumstances, it is often difficult to manage food sources that fulfill that person's ideal daily nutritional needs.
  • Nutritional supplements have emerged as a means to bridge up the gap between nutrients consumed through the every day intake of staple food eaten conventionally throughout the day and their recommended intake per day.
  • An illustrative list of such nutrients available through green leaves includes: chlorophylls, carotenes, xanthophylls, alpha tocopherols, phytosterols, highly bio- available calcium and iron, vitamin K, folic acid, poly unsaturated fatty acids containing the omega-3-alpha linolenic acid and importantly, also, high biological value dietary protein and dietary fiber. All these nutritional ingredients are individually very important part of nutritional requirements and some of them are likely to establish themselves as therapeutically effective to prevent or treat various diseases or disorders.
  • This invention has disclosed packaged nutritional supplements that have nutritional ingredients derived from green leafy vegetation that shall collectively or singly be useful to fulfill the daily need of supplementation from one or more of a nutrient from green leafy vegetation.
  • One embodiment of this invention discloses a novel packaged composition that comprises at least one fraction of a green leafy vegetation and at least one more ingredient to improve its usability, 100 gram of the composition on dry weight basis comprising at least one nutritional ingredient being selected from the group (a) dietary fiber at least 3 gram, (c) beta carotene at least 500 microgram, (d) iron at least 3 mg, (e) calcium at least 150 mg, (g) xanthophylls at least 4 mg, (h) folic acid at least 35 microgram, and (i) lysine at least 900 mg of and Methionine plus cystine at least about 550 mg; wherein the fraction comprises first three primary fractions as well as sub-fraction or a modified fraction of the three primary fraction of the green leafy vegetation; the primary fraction being obtained in a process of green crop fractionation, wherein the fractionation process comprises the steps of: (i) comminuting fresh green crop material, (ii) separating a moist fibrous fraction as first primary fraction from the juice as
  • Usability comprises property which facilitates use of the ingredient of the green leafy vegetation.
  • This is an edible composition
  • the use may be facilitated by modifying unfavorable taste, or unfavorable color, or unfavorable flavor or unfavorable solubility in water, or unfavorable dispersibility in water.
  • a carrier may be needed such as a petroleum jelly.
  • the contribution of at least one nutrient to the nutrient content of the composition from green leafy vegetation or its fraction is a least 15% of at least content of one nutrient of the composition.
  • the primary fractions mentioned above are either microbiologically stabilized before use as an ingredient in a dry mix or they are wet processed to make high moisture compositions, packaged and the packages are microbiologically stabilized either by sterilizing at 120° C under a pressure of 15 pounds per square inch for at least 20 minutes, or by adding permitted preservatives or deep freezing below 0° C to up to - 40° C or by expelling moisture until moisture content of the composition is below 10% up to 5%.
  • compositions of this invention comprising the fibrous fraction as at least one ingredient are illustrated by non-limiting examples of the following compositions: (a) composition A comprising: (i) fibrous fraction dry powder, (ii) one or more thickners enough to improve stability of suspension when diluted and brought to boil in a defined quantity of water under conditions of use as a soup, (iii) spices and condiments, (b) composition B comprising curd, made by adding fibrous fraction dry powder to milk, heating to boiling and cooling to room temperature, adding starter culture of curd, mixing once when curd is in initial stage of thickening and allowing the curd to form, (c) composition C comprising a cheese or paneer obtained by adding fibrous fraction dry powder to milk, heating to boiling and cooling the same to room temperature, curdling the milk, allowing excess water to drain off, pressing the curdled curd to remove excess fluid, (d) composition D of a "Ready To Eat" recipe comprising a mix of a cooked snack recipe and fibrous fraction dry powder.
  • compositions of this invention comprising deproteinized juice as at least one ingredient are illustrated by non-limiting examples of the following compositions: (a) composition E of bottled soft drink comprising carbonated or non-carbonated deproteinized juice, and one or more of additional ingredients for improved flavor, taste and a preservative, (b) composition F of a syrup comprising the deproteinized juice and one or more of additional ingredients for improved flavor, taste and a preservative, (c) composition G of a soft drink dry concentrate intended to be diluted before consumption comprising dry powder of the deproteinized juice and one or more of additional ingredients for improved flavor and taste, (d) composition H of a capsule or a tablet comprising dry powder of the deproteinized juice, excipients and fillers,
  • a composition of this invention comprising at least high protein low fiber fraction (LPC) or its sub-fraction, wherein the high protein low fiber fraction is a fourth fraction obtained in the a process of green crop fractionation described above comprised (a) LPC about 1 part of the composition, (b) a flour of parched wheat grains; and is added about 2 to 6 parts of the LPC, (c) flour of parched Grain Amaranth or a flour of puffed Grain Amaranth seeds; and is added about 2 - 8 parts of the LPC, (d) optionally garnishing of dry fruits, and (e) optionally containing one or more flavorings selected from the group comprising nutmeg, strawberry, cardamom, Vanilla, chocolate and Cacao.
  • LPC high protein low fiber fraction
  • composition of this invention for topical use for application to skin or hair for nourishment wherein the sub-fraction of the high protein low fiber fraction comprises an edible oil extract of LPC, to which is added a carrier for topical application and optionally fragrances.
  • the composition of this invention wherein the carrier is a hair oil further comprises petroleum jelly or another cream base or another hair oil base.
  • a novel packaged composition of this invention comprises pulp of whole edible green leafy vegetation and at least one component of flax-seed in the form of a soup or a food bar.
  • the component of flax seed may be one or more selected from the group of whole flax seed, flax seed cake or flax oil.
  • the green leafy vegetation used in this invention comprises a conventional green leafy vegetable or a forage crop or a by-product leaves of cole crops or leaves of legume crops or other non-toxic vegetation.
  • the ingredients of the nutritional supplement compositions of this invention are manufactured by a process of green crop fractionation. Green crop is processed to ensure that nutrients in the cells are released, isolated, packed and properly preserved to make them available for year round daily supply. Year- round availability of nutrients from green leafy vegetation / vegetables shall help to fulfill the daily need of vital supplementation of nutrients that are available from a portion of green vegetables in each meal. That may be useful to maintain health, control ageing, keeping vitality and reducing susceptibility to various diseases and disorders. For patients under treatment, an appropriate application of these supplements may contribute to stimulate better response to the treatment and speedy recovery to normalcy.
  • the supplementation from green leafy vegetables may be achieved when they are pulped while fresh to give a pulp or a homogenate so that majority of cells are broken open to release nutrients in the pulp and the wet pulp is used as an ingredient for making a nutritional supplement composition or a supplemented food preparation.
  • This method is limited to use of green leafy vegetables which do not have adverse tasting contents that get released after pulping in a predominant proportion.
  • green leafy plant species with toxic or undesirable ingredients in the vegetation can not certainly be not used for consumption as pulp. They are also not normally preferred for use through fractionation too.
  • Method of green crop fractionation illustrated by Pirie (1970) (Pirie N.W. Ed. "Leaf Protein: its agronomy, preparation, quality and use", IBP Handbook no. 20, published for the International Biological Program by Blackwell Scientific Publication, Oxford and Edinburgh, 1970) and Pirie (1978) (N.W. Pirie.
  • Leaf Protein Concentrate a protein- xanthophyll concentrate for supplementation of dietary proteins and carotenes and xanthophylls and to some extent considered useful for calcium and iron supplementation
  • fibrous fraction was seen as a cattle feed by-product
  • deproteinized juice DPJ was seen as another by-product mainly as a liability for disposal on account of its biological oxygen load.
  • DPJ was suggested variously as a fertilizer to be flown into a field, or as a medium for microbial growth.
  • the process of fractionation as described by Pirie (1970), comprising pulping the green leafy vegetation, pressing the juice out, coagulating the juice by various treatments, preferably by heat treatment, separating deproteinized juice from high- protein low fiber coagulum [variously called as Leaf Protein Concentrate (LPC), or Leaf Protein (LP)] is useful in preparing high protein fraction of green leafy vegetation that is primarily useful if the plant species is not toxic, or for preparing at least all fractions other than deproteinized juice if the plant species is toxic.
  • LPC Leaf Protein Concentrate
  • LP Leaf Protein
  • the green leafy vegetables themselves have continued to be least favored items in daily food consumption since many do not like their flavor and organoleptic taste and feel; and unfavorable organoleptic properties get further intensified to unfavorable side in their fractions.
  • the LPC had dark green color, becomes black and gritty when dried, when powdered it regains dark green color which is better than black color and texture is smoother than the dry grits; however, it has strong grassy flavor that is repulsive even for habitual vegetarians.
  • the scope of plant species that can be used comprises all green leafy vegetable plant species when whole pulp of the green leafy plant is envisaged to be used since people are already accustomed to eating them through thousands of years.
  • the scope of useable plant species extends to all non-toxic green leafy vegetation plant species. This includes most of the legume crops in tender stage of growth.
  • An embodiment of this invention comprises compositions derived from utilizing fractions of green leafy vegetation obtained by one or more of a following steps or a combination or a variation of them: (a) the green leafy vegetation is disintegrated, (b) its nutrients released from the cells, preferably in presence of at least one antioxidant in a quantity enough to prevent oxidative destruction of oxidizable active ingredients, and (c) the said nutrient is used as an ingredient of nutritional/food products or supplements or fractionated in several fractions and individual fraction, with or without further treatment, used as an ingredient of nutritional product/ nutritional supplement/ food product/ food supplement in conjunction with one or more of ingredients that would overcome its organoleptic or other property that is unfavorable for its consumption; wherein the said nutritional product/ nutritional supplement/ /food product/ food supplement has a shelf life longer than at least one day at a normal room temperature above 10°C and the same is not a traditional or conventional recipe.
  • inventional recipe does not refer to a kind of recipe but means a composition of ingredients that is traditionally eaten since a long time and has developed a well defined organoleptic perception in the population.
  • a 'conventional recipe does not include a recipe wherein the kind is conventional but has an ingredient that is different to mask the unfavorable organoleptic property of green leafy vegetation.
  • the said nutritional product/ nutritional supplement/ food product/ food supplement may conveniently be a packaged product and be shelf stable.
  • the said nutritional product/ nutritional supplement/ food product/ food supplement may also be a nutritionally labeled product. It is preferred that the nutritional composition of this invention comply as a source of at least one nutrient in a quanity that would be consumed per day.
  • a composition would be considered as "a source of a nutrient provided it supplied at least 15% of daily recommended intake/allowances (DRI/DRA) of the nutrient or conforms to well recognized threshold of minimum content in the composition.
  • DRI/DRA daily recommended intake/allowances
  • "Regulation (EC) No. 1924/2006 of the European Parliament and of the Council of 20 th December 2006” and Daily Recommended Allowances mentioned in Annex of "Council Directive of 24 September 1990 on nutrition labeling for foodstuffs (90/496/EEC) (OJ L 276,6.10.1990, page 40)” have been taken as a guide to define minimum nutritional standard of the composition of this invention.
  • the "green leafy vegetation” for the purpose of this specification may comprise green leaves or entire shoot with leaves or only green tender shoots or entire herbaceous plant without roots.
  • a conventional recipe may be itself so strongly flavored or has dominant organoleptic properties that incorporation of the fraction of green leafy vegetation masks the unfavorable flavor.
  • Mixtures of spices and condiments, known as Masla mixes are one non-limiting example of this embodiment.
  • the Masla mix shall very easily act as a carrier of fibrous fraction dry powder for adding to cooking of main dishes of vegetarian and non-vegetarian origin.
  • such a recipe shall be carrier of the desired fraction of a fraction of green leafy vegetation for integrating into the food consumption.
  • Incorporation of powder of fibrous fraction or LPC in a process of making a curd or cheese or paneer are examples of conventional recipe being a major carrier of the desired fraction overcoming its unfavorable organoleptic properties.
  • this invention comprises a process further comprising steps of: (I) green leafy vegetation is disintegrated to get a pulp and either used as such as an ingredient of a nutritional/food supplement/product, or (II) the pulp is processed further to separate juice from the fibrous fraction, (i) the fibrous fraction is preserved wet or dried and eventually used as an ingredient of nutritional/food supplement/product, with or without further processing, (ii) the juice is heat-treated and used without further fractionation or is treated to separate (a) a nutrient concentrate comprising proteins, fats and other water insoluble ingredients associated with them from (b) a deproteinized brown juice containing water soluble ingredients of the juice; and each of these fractions is used with or without further processing as an ingredient of a composition of a nutritional/food supplement; wherein the said nutritional/food products/supplements have a shelf life longer than at least one day at a normal room temperature above 10°C, and the same is not a conventional recipe.
  • the said green leafy vegetable is heated prior to subjecting it to step of disintegration.
  • the pretreatment may comprise a treatment that softens the tissue.
  • the pretreatment may be a heat treatment.
  • the heat treatment may be given by immersion of the green leafy vegetable in boiling water for a period of time.
  • the pretreatment may also be by an autoclaving treatment i.e. by subjecting the green leafy vegetable to a steam pressure of about 15 psi (pounds per square inch) at 121° celcius.
  • the disintegration may be made using commonly available mechanical blenders, pulverizers or by making special purpose machines that apply shearing force to the vegetation so that it leads to mechanical maceration of the tissue of the vegetation resulting in a pulp.
  • the nutrient concentrate never came up as a practical nutritional / food supplement mainly because it is a water insoluble dark green product having a grassy flavor which are considered basically as unappealing organoleptic properties by themselves, and further, they are transferred and expressed in the conventional recipes in which they are incorporated.
  • Unfavorable organoleptic properties has remained a major obstacle in practical widespread use of the nutrient concentrate derived from green leafy vegetation.
  • the other two fractions i.e. fibrous fraction and de-proteinized fluid were never perceived to any practical application for human consumption and have been perceived as bye-products that need to be disposed off.
  • Savangikar & Savangikar (WO/2008/081472) for the first time in 2006 envisaged processing the fibrous fraction as well as deproteinized juice to stabilize them as fractions useable for human consumption further and proposed them as useful for the first time for human consumption.
  • making shelf stable palatable supplements from them was still a task that was left to be achieved for the future.
  • the present invention comprises a nutritional/food supplement that comprises at least one nutritionally active component liberated from macerated and disintegrated tissue of a green leafy vegetation and at least one more additional ingredient that makes the composition organoleptically palatable and novel products.
  • nutritionally active component liberated from macerated and disintegrated tissue of a green leafy vegetation is nutrient concentrate.
  • the said additional ingredient comprises mixture of parched wheat flour and puffed grain amaranth. Parching of wheat and puffing of grain amaranth improves their digestibility on one hand and imparts an attractive taste and flavor. It was seen that dispersibility of LPC in the compositions intended to be diluted with water under conditions of use was improved by using these flavor enhancing ingredients which also gave appropriate thickness to the composition.
  • Flavor giving ingredients such as puffed Amaranth seeds had lesser ability to give thickness and puffed rice had practically no ability to give thickness, however, appropriate combinations could be worked out that gave flavor of all of them and also thickness required to keep the LPC particles afloat under conditions of use. Many other equivalent combinations and ingredients may be used to achieve same effect. They are mixed in such a proportion with respect to nutrient concentrate that there is mutual supplementation with respect to essential amino acids to make it as close as possible to the Essential Amino Acid composition of F.A.O./W.H.O. Reference Protein (1965) [Protein Requirements. F.A.O. Nutr. Mtg. Rep. Ser. No. 37 (Food and Agricultural Organization, Rome)].
  • This mixture could be consumed as such, or with a sweetener as a solid powder.
  • This mixture could also be consumed by turning it to a paste or a thick fluid beverage by diluting with water or milk and adding a sweetener to give a very agreeable mouth-feel, flavor and taste.
  • This could also be consumed as salty or/and hot recipe with buttermilk.
  • the unfavorable impact of dark green color of the particles of the nutrient concentrate (LPC) gets substantially reduced and in fact becomes appealing as a green supplement on the white to cream colored back ground of particles of wheat flour and puffed green amaranth.
  • wheat flour may be partly or fully be replaced by flour of other cereals, puffed or otherwise.
  • Other ingredients having moderately good lysine or/and good to rich sulfur amino acids (Methionine and cystine) and contributing to a favorable taste and flavor, including but not limited to Sesame may also be added to varying extents.
  • Permitted colors, permitted flavors including Coco, Chocolate and Cardamom, and taste and flavor improving adjuvants including pieces of various dry-fruits may also be added to the composition.
  • small quantities of other nutritional ingredients can be added as fortifications including but not limited to milk solids, vitamins, minerals, taste-masked compositions of omega-3-fatty acids including Alpha Linolenic acid, Eicosapantenoic acid and Docosahexaenoic acid.
  • sweeteners including high intensity sweeteners can be added; or salty recipes can be prepared in buttermilk as a diluent.
  • the fraction to which a food ingredient is added could be the fibrous fraction that remains after juice is separated from the wet ground pulp of the green leafy vegetation.
  • a fibrous fraction is a green mass which can be dried and milled. If wet grinding is done in a hammer mill a good proportion of fiber would already be in a finely divided state and may be usable directly after isolating from other constituents with or without drying.
  • Fibrous fraction is extremely unpalatable, dry, repulsive ingredient in the mouth that is also irritating to throat if attempted to be consumed without any adjuvants that would make the mixture palatable.
  • composition of this invention containing fibrous fraction a curd was prepared after mixing with milk and adding an inoculum of curd (lactobacilli culture). Taste of this curd was very good, it could be consumed as such as well as with other constituents of a meal without getting dry, fibrous or irritating feel in the mouth or in the throat.
  • adding at least one dry food ingredient including but not limited to, rice, pulses, dried tomato powder and condiments including dried onion powder, dried garlic powder, "Ready-to-make" mixes of Instant Soup preparation were prepared. Thickening agents gave a thickened constancy that gave a good stable suspension property to the soup so that the fiber particles remained afloat and did not segregate and settle.
  • Edible gums are preferred since they would also improve the nutritional property of the composition by adding soluble fiber too, which is needed for good health
  • Such ready-to-make mixes could be consumed as soup after diluting by defined quantity of water, boiling for about three minutes. These soups were delicious and fiber in it got well blended with other constituents of the soup and did not irritate the throat nor did they give a fibrous feeling when the soup was consumed.
  • a soup is ideally consumed at the start of the meal. This is most appropriate method of consumption of fiber because its benefits with respect to adsorption of fat and cholesterol, regulating movement and speed of movement of food in the digestive tract and its pre-biotic action would be best available when it is mixed with the food eaten at the time of meal. This can be useful to make nutritional bars.
  • fiber For use as an ingredient in food supplements, fiber could be used wet preserved or could be dried, pulverized and used as an ingredient.
  • High fiber soups are useful for making up deficiency in consumption of daily quota of dietary fiber. This correction leads to health benefits which are well known, for known or unknown reasons, including, but not limited to, improved gastro-intestinal motility, reduction or prevention of constipation, reduction in cholesterol, reduction in triglycerides, prevention of weight gain etc.
  • This invention discloses, in an another embodiment, a soup or a bar incorporating a green leafy vegetable and/or water insoluble fraction of the same as an ingredient.
  • a soup or a food bar in which whole vegetable or water insoluble constituents are as ingredient has a significance from the nutritional point of view as a food supplement containing green leafy vegetable. From dietetic point of view, a 100 gram portion of green leafy vegetable is a must with lunch and dinner for the purpose of getting several nutrients in small quantities that are essential for maintaining good health. Green leafy vegetables are rarely available every day.
  • This invention also discloses a process of preparing a soup or a bar from a green leafy vegetable or water insoluble fraction of the same.
  • the present invention comprises a nutritional/food supplement that comprises at least one nutritionally active component liberated from macerated and disintegrated tissue of a green leafy vegetation and at least one more additional ingredient that makes the composition organoleptically palatable and novel products.
  • the said process of preparing a soup or a bar from a green leafy vegetable or water insoluble fraction of the same comprises giving heat treatment to the raw material for softening the tissue.
  • the said heat treatment may comprise a blanching treatment in boiling water or at 121° C under 15 pounds per square inch.
  • the said heating treatment is followed by pulping with addition of some quantity of water, if required to facilitate pulping. Water solubles from the pulp are removed, if desired.
  • Pulp of heat treated raw material is smooth, gelatinous and thick. This can be passes through a mesh of about 3 mm diameter holes to ensure that any big pieces or fiber that has not disintegrated is held back.
  • ingredients of the instant soup mix are added, heated to boil and kept boiling for about two minutes to get the soup.
  • the soup is thick, the fiber gives a thick gelatinous body to the soup.
  • This product may be prepared as autoclaved / pressure cooked ready-to-drink soup.
  • the mix of pulp and instant-soup mix can also be filled up in pouches and given autoclaving treatment in a retort.
  • the soup when concentrated further to such an extent that it contains some moisture but can be given a form, resulted in bars, like food bars. These bars can be of any shape such as flat rectangular bars, or cubes or any other convenient type. These bars were also palatable.
  • Soup and bar prepared from Spinach by above described method were tasty.
  • soup and bar prepared from Fenugreek by same methods were extremely bitter. When tasted, it was realized that it is its water soluble portion that was bitter. Hence, it was decided to get rid of the water soluble part and then make a soup or bar. Accordingly, raw vegetation was pulped, pressed to separate juice form the fibrous fraction. Fibrous fraction was free from bitterness. This fibrous fraction was autoclaved for 20 minutes with water sufficient to immerse the same and pulped. However, it was surprising that the fiber, after autoclaving did not give subdivided pulp that could pass through 3 mm mesh. The resulting pulp was extremely coarse and was not palatable.
  • Methi vegetation was autoclaved for 20 minutes and pulped with some quantity of added water. This gave smooth pulp that could pass through 3 mm sieve and was palatable.
  • Water was separated from the fiber. This was done by pressing through a coarse cotton cloth. Various other methods can be used for this purpose including centrifugation. The fiber was a fine and palatable mass. To this water and instant soup mix was added and boiled for two minutes. This soup and bar prepared from it had far less bitterness. If total removal of bitterness is desired, one water wash to the separated fiber may achieve this objective.
  • the oil extracted fraction of the fourth fraction i.e. oil extracted fraction of LPC was dried and stored for further use or could be mixed with bulk ingredient of desired compositions, such as breakfast cereals or parched wheat flour so that its moisture content was brought down rapidly and both the components were dried together.
  • the fraction used as an ingredient of a nutritional composition can be deproteinized brown juice. It contains all water soluble nutrient actives present in green vegetation such as folic acid. It may also contains water soluble polyphenols and their derivatives. When it is prepared from a green leafy vegetable or vegetation that does not contain anti-nutritional factors such as saponins, alkaloids etc. deproteinized brown juice can form an ingredient of nutritional supplements. It has an attractive sparkling golden yellow or golden brown color and it can be converted into a health soft drink, a lifestyle drink such as a cola drink with healthy ingredient by adding other ingredients such as, without limitation, a food acid, a sweetener and a flavoring ingredient. The cola drink may also be carbonated.
  • deproteinized brown juice may be concentrated to a thick syrup.
  • This syrup is micro-biologically stable at room temperature for at least up to one year.
  • This may be used as an ingredient for several purposes and products, including but not limited to, as a flavoring ingredient in cooking recipes such as soups, vegetables, or mixed with excipients to make flavored and good tasting syrups as nutritional supplements or may be converted to a solid powdered form by further drying or by adding to it and mixing with a powder of an excipient resulting into a free flowing solid powder which can be either filled in a capsule or compressed into a tablet using standard technology of capsule making or tablet making.
  • Syrup and dry powder of deproteinized juice fraction can be used as an ingredient of a soft drink concentrate that can be diluted with defined quantity of water before consumption. Dry powder soft drink concentrate may also be made as an effervescent drink after dilution when a dry alkali and a dry acid is added to the mix. Shelf stability is achieved conveniently by reducing moisture content of the edible composition to less than 10%, preferably to 8%, more preferably to around 6%. When the recipe is such that it has to be high in moisture, such as but not limited to curd, bread spread, sandwich spread, cheese, paneer , pudding, sauce, the recipe shall be packaged and stored at refrigeration or freezing as appropriate until consumed. Packaging and shelf stability is considered an essential attribute since it is in this form that the compositions of this invention shall be industrially applicable.
  • pulp of whole vegetable For plants with unpleasant flavor components in the juice, when pulp of whole vegetable is proposed to be sued, for example in case of Fenugreek, the pulp can be pressed to remove the water solubles, fraction washed with one or more washes of water, used further to make a recipe such as a soup with addition of flax seed to improve taste as well as nutritive value on account of its content of alpha linolenic acid fractions in its oil.
  • the expressed water from the pressed autoclaved vegetation can be used after concentrating by converting into a drink by adding flavor modifiers or may be concentrated, made into a syrup with adding flavoring substances or dried altogether, encapsulated and consumed as supplements of water soluble nutrients of those plant species, unless the water solubles contain any toxic element, in which case this fraction is totally discarded.
  • Edible oil extract of LPC can simply be converted to gelatin capsule in the range of 100 milligram to 1000 milligram. Ingredients that give stable suspensions with LPC and flavor masking effect on LPC would give same effect over insoluble and grassy flavored fibrous fraction too.
  • Green leafy vegetation Edible green leafy vegetables available in market were purchased for this work, washed clean under running water, root portion cut off and tender shoot portion with leaves (designated as "vegetation") was taken for further work. Green leafy vegetables selected were Spinach (Spinacea oleracea L), Fenugreek (Trigonella graecum L), Lucenrne (Medicago sativa L.) and cowpea (Vigna unguiculata L). Protocols for below mentioned recipes were followed/repeated in same manner of all these three plant species mentioned above.
  • Parched flour of wheat or rice or Grain Amaranth The respective grains were parched and then milled to get flour.
  • Puffed Grain Amaranth This was done by parching the grains of Amaranth by subjecting it to heat treatment in a utensil until it puffs suddenly.
  • Puffed rice Rice is also puffed in a similar way, but usually in a kiln as a commercial operation. The puffed rice is usually salted and was purchased from grocery shop.
  • Raw vegetation pulping The vegetation was cut into small pieces about one inch in length, the pieces were charged to the jar of a domestic mixer grinder driven by electricity. Water was added just enough to avoid bridging of the charge and pulping was done until fine pulp was obtained.
  • Moist fibrous fraction was separated as one primary fraction from the juice, the second fraction by pressing through a cloth.
  • Juice is added to a pan holding boiling water at such a rate that the temperature did not fall below 80°C at any time to get a coagulated juice.
  • the coagulated juice was filtered through a cotton cloth to separate a water soluble deproteinized juice that is high in water content as third primary fraction from a water insoluble high protein low fiber fraction as fourth primary fraction in the form of a curd which was pressed through a cloth to remove residual deproteininzed juice.
  • the moist fibrous fraction was washed with hot water, excess water removed and then washed fibrous fraction was dried in draft oven at 80°C and pulverized in a pulverizer to pass though a mesh having pore size of 1 mm. to get a powder.
  • the powder often had insoluble fiber content of 55% and soluble fiber content of 0.5%.
  • this fraction as a dry powder was unpalatable when made from any plant species, it was insoluble, had coarse texture, could not be consumed either by chewing or by drinking with a fluid as it gave irritating fibrous feeling to throat.
  • the fourth fraction water insoluble high protein low fiber fraction was, depending on plant species used, of light green or dark green color about 45 to 60 % protein, 10- 15% crude fat, about 5% ash (about 1% acid insoluble ash), less than 1% fiber and rest carbohydrates.
  • This fraction was either dried and stored for further use or was extracted with flax oil by passing flax oil through a column of loosely packed moist curd, the oil separated from the curd by pressing through a cloth and then by adding water to the fraction so that loosely bound residual oil separated from the curd and was skimmed off; giving two sub fractions of the fourth fraction (a) sub-fraction 1 comprising oil enriched with oil soluble components of the curd comprising carotenes, xanthophylls, polyunsaturated and other fats, alpha tocopherol and other oil soluble constituents of the curd, and (b) oil extracted fraction of the fourth fraction which was dried and stored for further use or could be mixed with bulk ingredient of desired compositions, such as breakfast cereals so that its moisture content
  • This fourth primary fraction had, however, unfavorable organolptic properties such as grassy flavor, poor dispersibility in beverages, dark green color and gritty texture when dried. It imparted unfavorable and often unacceptable taste or appearance or flavor to all conventional recipes in which it was incorporated.
  • the deproteinized juice (DPJ) is separated from the LPC by centrifugation.
  • the DPJ is used as such in formulations or is concentrated to a syrup by application of heat and further dried in draft oven at 80° C . This is also a fraction rich in minerals and polyphenols which are useful as nutritional supplements.
  • Grassy flavor of fiber was masked by adding spices and condiments, tomato powder, garlic powder, citric acid etc. Masking Dark green color, gritty texture and grassy flavor of the fourth fraction - water insoluble high protein low fiber fraction LPC or its sub-fraction, and grassy taste of green leafy vegetables:
  • parched wheat flour, parched grain amaranth, puffed grain amaranth and puffed rice had an agreeable aroma even when eaten dry without any further additives. These were selected as flavor modifiers for recipes prepared from the fourth primary fraction i.e. water insoluble high protein low fiber fraction and/or fibrous fraction fraction.
  • Combinations of 1 gram LPC to 1 , 3, 6 and 10 gram each of parched wheat flour, parched grain amaranth, parched rice flour, puffed grain amaranth and puffed rice were made. These mixtures were added to 100 ml water each, mixed well, then heated to boil and poured in vertical glass containers of 125 ml capacity. Rate of sedimentation and segregation of the mixture in different layers was observed. It was found that rice flour and puffed rice failed to prevent sedimentation and segregation of the mixture in all the combinations.
  • Root portion of the vegetation was cut off, which removes about one third of the plant body, 100k of was washed thoroughly with water followed by cooking in boiling water for 20 minutes or in an autoclave at 120° C at 15 psi for 20 minutes. The vegetation was then pulped in a domestic kitchen grinder mixer to get a pulp.
  • pulp from autoclaved vegetation was preferred for all plant species.
  • Soup or food bar from the pulped vegetation To a pulp from 100 gram fresh vegetation, was added an 8 gram mixture of a soup composition as given in Table 1 except the fiber component, the mixture was boiled for 2 to 3 minutes to convert it into a soup and was either deep frozen or packaged in a polymer film pouch that can withstand autoclaving and autoclaved. Alternatively, the boiled mixture was spread as thin sheet and heated further cautiously to achieve drying without charring. When moisture content was low enough, the mass was scraped off, made into a bar and the bar was dried further.
  • compositions from fractions of Green Leafy Vegetation Breakfast Cereals, beverages and snacks: a) A mix of Parched wheat flour, LPC and Parched Grain Amaranth or Puffed and powdered Grain Amaranth was prepared in various proportions with small quantity of puffed rice occasionally. Proportion of LPC as 10% in all combinations, 90% being contributed by other ingredients. This could be packaged and was microbiologically stable for more than one year.
  • the attractive flavor of parched wheat flour combined with attractive flavor of parched Grain Amaranth flour or puffed Grain Amaranth flour largely masked the grassy flavor of LPC except some agreeable flavor of the original vegetable, which was agreeable since it was familiar to the people.
  • the mixture could be eaten raw also with addition of desired sweetener, it could also be consumed as a beverage when water or milk or buttermilk was added in a proportion of 125 ml to each 10 gram mixture and brought to boiling, and sweetener or salt added to it as per individual taste.
  • the resulting suspension was stable for a reasonably long time for a person to consume it and appeared brown with green particles stably floating throughout. Appearance of the recipe was white in dry condition also with light green particles dispersed throughout.
  • the nutrient content of this formulation was, per 100 gram dry matter, fiber 8%, protein 18% of energy value of 100 gram composition, calories 375 kcal, calcium 495, iron 33 mg.
  • Various breakfast items were prepared using LPC extracted from all crop plants illustrated in this work.
  • the items prepared were 1) Indian rice-pulse mix ( Khichadi in local language) where a mix of LPC and other cereal ingredient like whole wheat, rice, moong dahl (Vigna radiate L), oats , ground flax , amaranth, spices and condiments are added so that the beta carotene 700 microgram.
  • the soup compositions were tasted by a panel of 5 trained judges who recorded their assessment of whether the taste is acceptable or not by "yes" or "no".
  • the majority decisions are recorded below for the illustrative soup compositions.
  • the soup compositions recorded as "no" were generally bitter when the fibrous fraction was from Fenugreek or were not agreeable in general taste when the fibrous fraction was from vegetables other then Fenugreek.
  • Table 1 Various Compositions of soup with fibrous fraction dry powder as an ingredient
  • Proportion if fibrous fraction that could be used depended on other constituents used as well as on the plant species.
  • Various ready-to-use mixtures of spices (Masala mixes, as called in local vernacular language Marathi in India) were made from fibrous fraction which are added as condiments during cooking to add flavor and taste of various salty recipes from vegetarian as well as non-vegetarian main ingredients such as pigeon pea dalhs, poultry, mutton, beef, pork, non-leafy vegetable etc. Usually these condiments are meant for making these recipes tasty.
  • These mixtures of spices, Masala mixes would serve as excellent vehicles for adding fiber in palatable form in the recipes for improving consumption of dietary fiber.
  • the fiber from all illustrative vegetable used in this study are mixed with mixture of ground and powdered spices mix.
  • Fiber rich curd Dry powder of from 2 to 6 gram of fibrous fraction was washed with water to remove residual solubles, was added to 100 milliliter of fresh milk, brought to boil, cooled to room temperature, starter culture of lactobacillus derived from small portion of traditionally prepared curd was added to it and allowed to form a curd in course of time at room temperature. When the mixture started thickening in initial phase of curd formation, it was mixed to ensure that the powder of fibrous fraction mixed uniformly throughout. At this stage, the powder of the fibrous fraction did not settle. This gave curd fortified with fibrous fraction.
  • This fiber fortified curd was very much agreeable and tasty and could be eaten as such or with other recipes without getting any disagreeable flavor or disagreeable texture or a disagreeable taste of the fibrous fraction component.
  • the fiber fortified curd done in this way contained at least 3 gram dietary fiber per 100 gram dry matter. Proportion of fiber per 100 ml can be increases even until it makes a thick paste into which the starter can be mixed, milk portion allowed to curdle so that after adding condiments to it, such a curdled paste can be used as a bread spread or as a spread in a sandwich. These compositions can be packaged and would keep for a long time at refrigeration temperature or when deep frozen.
  • Fiber rich Paneer or cheese Fiber rich milk prepared for above fiber rich curd was curdled by adding various ways such as adding a food acid or salt or lime juice or a proteolytic enzyme. The curdled portion was separated by pressing through cloth and converted to firm slab which was cut into convenient pieces. This gave pieces of Paneer fortified with fibrous fraction and contained at least 3 gram fiber per 100 gram dry matter. This tasted agreeable and could be very easily used to add to vegetables. It would also be possible to raise proportion of fiber in the recipe until it starts becoming unpalatable when the fiber fortified paneer is added to other recipe. When such a stage would be reached will vary with individual and also with the cultural background.
  • Carotenoid rich milk, curd, paneer and cheese Enriched Oil i.e. edible oil extract of the fourth fraction that is "high protein low fiber fraction" (LPC) rich in carotenoid and chlorophyll was added at various levels in milk until it was no longer able to emulsify and the milk could be consumed as such, or could be made into curd by adding starter culture of curd or made into paneer by adding a curdling agent. Milk could either be packaged in conventional way after pasteurization and consumed as such; or it could be converted into curd, paneer or cheese which could be packaged and kept at refrigeration temperature until consumed.
  • LPC high protein low fiber fraction
  • the syrup could also be converted into concentrate of soft drink concentrate by mixing 200 mg dry powder of deproteinized juice to a commercially available soft drink concentrate that contained dry concentrate of orange juice, orange oil as flavoring agent, glucose, lactose, minerals, preservatives, emulsifiers and stabilizers. This could be diluted to get sweet tasty soft drink.
  • Soft drink could also be prepared by adding 200 mg dry powder of deproteinized juice to a commercially available salty soft drink concentrate that contained black salt, dextrose, citric acid, ginger, cumin, mint and green chilli. This could be diluted to get salty and tasty soft drink which could be consumed with food.
  • Deproteinized juice 30 ml was added with above commercially available soft drink concentrates, boiled, cooled and they tasted very well and could be bottled, g) Oil can form as good nourishment for the skin and find application as a skin cream.
  • the 2 ml of oil extract of LPC is mixed in 2 gram petroleum jelly to form a good skin nourishing cream.
  • the oil extract can be made to form gelatin capsules.
  • the oil extract in each capsule can range from 200 mg to 1g.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

La présente invention concerne des suppléments nutritionnels conditionnés dont les ingrédients nutritionnels sont issus de légumes verts à feuilles, lesdits ingrédients pouvant être utilisés collectivement ou isolément pour satisfaire les besoins quotidiens en supplémentation en un ou plusieurs des nutriments issus des légumes verts à feuilles. L'invention concerne, selon un mode de réalisation, une composition conditionnée inédite contenant au moins une fraction d'un légume vert à feuilles et au moins un autre ingrédient visant à améliorer sa capacité d'utilisation, ladite composition constituant une source d'au moins un ingrédient nutritionnel choisi dans le groupe des fibres alimentaires, du bêta-carotène, du fer, du calcium, des xanthophylles, de l'acide folique et de la lysine, plus de la méthionine et de la cystine. Ladite fraction comprend une fraction fibreuse, une fraction juteuse déprotéinée et soluble dans l'eau et une fraction à faible teneur en fibres et à forte teneur en protéines insoluble dans l'eau en tant que quatrième fraction principale. Dans un mode de réalisation de l'invention, la contribution d'au moins un nutriment à la teneur en nutriments de la composition issue de légumes verts à feuilles ou d'une fraction de ceux-ci est au moins égale à 15 % d'au moins la teneur en un nutriment de la composition. L'invention concerne également une composition conditionnée inédite contenant de la pulpe de légumes verts à feuilles comestibles entiers et au moins un composant de type graines de lin, ladite composition se présentant sous la forme d'une soupe ou d'une barre alimentaire. Le composant de type graines de lin peut correspondre à des graines de lin entières et/ou à des tourteaux de lin et/ou à de l'huile de lin.
PCT/IN2011/000591 2010-08-30 2011-08-30 Suppléments nutritionnels issus de légumes verts à feuilles WO2012029075A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/819,903 US9993018B2 (en) 2010-08-30 2011-08-30 Nutritional supplements from green leafy vegetables
EP11767305.3A EP2611311A1 (fr) 2010-08-30 2011-08-30 Suppléments nutritionnels issus de légumes verts à feuilles
US15/990,534 US20180271133A1 (en) 2010-08-30 2018-05-25 Nutritional supplements from green leafy vegetables

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2411MU2010 2010-08-30
IN2411/MUM/2010 2010-08-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/819,903 A-371-Of-International US9993018B2 (en) 2010-08-30 2011-08-30 Nutritional supplements from green leafy vegetables
US15/990,534 Continuation US20180271133A1 (en) 2010-08-30 2018-05-25 Nutritional supplements from green leafy vegetables

Publications (2)

Publication Number Publication Date
WO2012029075A1 true WO2012029075A1 (fr) 2012-03-08
WO2012029075A4 WO2012029075A4 (fr) 2012-06-28

Family

ID=44773109

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2011/000591 WO2012029075A1 (fr) 2010-08-30 2011-08-30 Suppléments nutritionnels issus de légumes verts à feuilles

Country Status (3)

Country Link
US (2) US9993018B2 (fr)
EP (1) EP2611311A1 (fr)
WO (1) WO2012029075A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014102835A1 (fr) * 2012-12-31 2014-07-03 Chitra Vasant Savangikar Produits de boulangerie, boissons et concentrés pour boisson à haute teneur en fibres
WO2017168453A1 (fr) * 2016-04-02 2017-10-05 Chitra Vasant Savangikar Fractions végétales ayant des propriétés anti-pathogenèse
BE1025437B1 (nl) * 2017-07-27 2019-02-27 Agrafresh Bvba Werkwijze voor het vervaardigen van een soepbasis

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106235341A (zh) * 2016-07-30 2016-12-21 南京正宽医药科技有限公司 一种竹笋膳食纤维提取方法和治疗便秘的颗粒剂
EP3591660A1 (fr) * 2018-07-05 2020-01-08 Riprup Company S.A. Procédé d'évaluation d'une pluralité de recettes d'eau et procédé de sélection d'une recette d'eau
RU2758290C1 (ru) * 2020-12-08 2021-10-28 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный технологический университет" (ФГБОУ ВО "КубГТУ") Способ приготовления супа-пюре
CN114391618B (zh) * 2022-01-14 2023-07-25 湖南苋鲜生态科技有限公司 一种草鱼饲料的制备方法和混合方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976548A (en) * 1994-11-08 1999-11-02 Viva America Marketing, Inc. Nutritional supplement composition and use
US20040001817A1 (en) * 2002-05-14 2004-01-01 Giampapa Vincent C. Anti-aging nutritional supplement
US20050266018A1 (en) * 2004-05-27 2005-12-01 Boreyko Benson K Nutraceutical compositions with mangosteen
WO2008081472A1 (fr) 2006-12-28 2008-07-10 Chitra Vasant Savngikar Production intégrée de produits ou d'isolats phytochimiques de plantes riches, issus de végétation riche
CN101647480A (zh) * 2008-08-15 2010-02-17 张辉 一种菠菜面条

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2192139C2 (ru) * 2000-11-03 2002-11-10 Самохин Сергей Борисович Способ производства соевого белкового продукта, преимущественно соевого сыра

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976548A (en) * 1994-11-08 1999-11-02 Viva America Marketing, Inc. Nutritional supplement composition and use
US20040001817A1 (en) * 2002-05-14 2004-01-01 Giampapa Vincent C. Anti-aging nutritional supplement
US20050266018A1 (en) * 2004-05-27 2005-12-01 Boreyko Benson K Nutraceutical compositions with mangosteen
WO2008081472A1 (fr) 2006-12-28 2008-07-10 Chitra Vasant Savngikar Production intégrée de produits ou d'isolats phytochimiques de plantes riches, issus de végétation riche
CN101647480A (zh) * 2008-08-15 2010-02-17 张辉 一种菠菜面条

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"IBP Handbook no. 20", 1970, BLACKWELL SCIENTIFIC PUBLICATION, article "Leaf Protein: its agronomy, preparation, quality and use"
"Protein Requirements. F.A.O. Nutr. Mfg. Rep. Ser. No. 37", 1965, FOOD AND AGRICULTURAL ORGANIZATION
DATABASE WPI Week 201017, Derwent World Patents Index; AN 2010-C41597, XP002663785 *
LEXANDER K., CARLSSON R., SCHALEIN V., SIMMONSON A, LUNDBORG T.: "Quantities and qualities of leaf protein concentrates from wild species and crop species grown under controlled conditions", ANN.APPL.BIOL, vol. 66, 1970, pages 193
N.W. PIRIE: "Leaf protein and other aspects of fodder fractionation", UNIVERSITY PRESS
SAVANGIKAR V.A., JOSHI, R.N.: "Edible protein from Parthenium hysterophorus L", EXPERIMENTAL AGRICULTURE, vol. 14, no. 1, 1978, pages 93 - 84

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014102835A1 (fr) * 2012-12-31 2014-07-03 Chitra Vasant Savangikar Produits de boulangerie, boissons et concentrés pour boisson à haute teneur en fibres
WO2017168453A1 (fr) * 2016-04-02 2017-10-05 Chitra Vasant Savangikar Fractions végétales ayant des propriétés anti-pathogenèse
CN108883188A (zh) * 2016-04-02 2018-11-23 C·V·萨万基卡尔 具有抗致病性质的植物级分
BE1025437B1 (nl) * 2017-07-27 2019-02-27 Agrafresh Bvba Werkwijze voor het vervaardigen van een soepbasis

Also Published As

Publication number Publication date
US9993018B2 (en) 2018-06-12
US20130156894A1 (en) 2013-06-20
EP2611311A1 (fr) 2013-07-10
WO2012029075A4 (fr) 2012-06-28
US20180271133A1 (en) 2018-09-27

Similar Documents

Publication Publication Date Title
US20180271133A1 (en) Nutritional supplements from green leafy vegetables
KR100914065B1 (ko) 야생초를 이용한 선식과 이를 이용한 선식음료
KR20130041388A (ko) 치아 종자 조성물
Kishor et al. Nutritional composition of chickpea (Cicer arietinum) milk
KR102381605B1 (ko) 마라장의 제조 방법
Ekesa Selected superfoods and their derived super diets
KR20150077483A (ko) 백색식품 및 흑색식품 재료를 이용한 선식 및 그의 제조방법
KR101115450B1 (ko) 차와 죽의 기능이 함께하는 일회용 시리얼 조성물 및 그 제조방법
US9161960B2 (en) Horse feed dietary supplement composition derived from chia seed composition
Lusas et al. Food uses of whole oil and protein seeds
JP2016034239A (ja) 健康食品
KR20190028931A (ko) 고소애 에너지 바의 제조방법 및 이에 의해 제조된 고소애 에너지 바
KR20180093462A (ko) 장어뼈가 포함된 개암 죽의 제조방법
CN111616220A (zh) 一种瘦身饮品及其制备方法
KR20210050351A (ko) 배 양갱 제조방법 및 그에 따라 제조된 배 양갱
KR101547576B1 (ko) 기능성 곡물 및 그 제조방법
CN103859392A (zh) 一种低脂食品、馅料及制备方法
KR101571402B1 (ko) 12곡 영양곡물분을 이용한 쌀호떡 및 그 제조방법
KR102464983B1 (ko) 다이어트용 면 및 그 제조방법
KR100607915B1 (ko) 잣뽕잎수제비 및 그 제조방법
Swaminathan Oilseed and nut proteins
John et al. Nutrition and dietetics
KR20180085601A (ko) 다이어트식품 조성물
KR100522415B1 (ko) 생야채 비빔밥
Campos-Vega et al. Designer food and feeds from underutilized fruits and vegetables

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11767305

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 13819903

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2011767305

Country of ref document: EP