WO2011116649A1 - 培育北虫草的方法 - Google Patents

培育北虫草的方法 Download PDF

Info

Publication number
WO2011116649A1
WO2011116649A1 PCT/CN2011/071256 CN2011071256W WO2011116649A1 WO 2011116649 A1 WO2011116649 A1 WO 2011116649A1 CN 2011071256 W CN2011071256 W CN 2011071256W WO 2011116649 A1 WO2011116649 A1 WO 2011116649A1
Authority
WO
WIPO (PCT)
Prior art keywords
cordyceps militaris
cordyceps
liquid
rice
temperature
Prior art date
Application number
PCT/CN2011/071256
Other languages
English (en)
French (fr)
Inventor
陈顺志
Original Assignee
江苏学府生物工程有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 江苏学府生物工程有限公司 filed Critical 江苏学府生物工程有限公司
Publication of WO2011116649A1 publication Critical patent/WO2011116649A1/zh

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Definitions

  • the invention belongs to the technical field of agricultural technology and traditional Chinese medicine technology, especially the cultivation and processing of edible and medicinal fungi.
  • Edible medicinal fungi are closely related to human health, such as dozens of fungi such as ganoderma lucidum, white fungus, monkey head, shiitake mushroom and cordyceps.
  • Cordyceps is a type of entomogenous fungus that is parasitic on larvae. There are about 400 species on the earth. Various cordyceps choose different larvae to parasitize. The earliest Chinese Cordyceps sinensis is parasitic on bat moth larvae, and its production area is limited to high altitude and high cold areas, namely Qinghai, Cambodia, Gansu and Sichuan in China.
  • Cordyceps sinensis has higher medical value.
  • Cordyceps sinensis is also known as Cordyceps militaris, Cordyceps militaris, Cordyceps militaris, and valerian, which are parasitic in the corpus callosum.
  • the artificial cultivation method of Cordyceps militaris has been pretreated in the production process by retort sterilization. This method consumes a lot of heat and takes a long time. 100 ° C, atmospheric pressure 8 10 small ⁇ ; 127, 1 kg of high pressure also takes 2 hours.
  • packaging containers such as glass bottles and plastic bottles also raise the temperature.
  • the invention relates to a method for cultivating Cordyceps militaris with industrialized energy-saving and low-carbon, and relates to a method for cultivating edible and medicinal fungi, and a technology for energy-saving, low-carbon and automatic production by expanding the key technology of the raw material of the culture medium.
  • 200410059964.X Cordyceps sinensis food and Cordyceps militaris substrate and its preparation method. It adopts Cordyceps sinensis food made from Cordyceps sinensis with cooked rice flour, beef throw, vegetable oil, sucrose and other ingredients.
  • the ingredients are commonly used ingredients for rice flour crystal foods or puffed foods, xylitol sugar, lecithin, citric acid, hydrogen carbonate pin, vanillin, shortening, salt, antioxidant.
  • Cordyceps militaris can be prepared or purchased by itself.
  • Cordyceps substrate is selected from high-quality northeast rice, potatoes and carrots. After high sterilization, it is put into the bottle for use.
  • the liquid culture of Cordyceps militaris is mixed.
  • the nutrient solution is inoculated into the flask; the rice powder crystal or rice crack can be prepared by the light-proof fermentation culture, the astigmatism fermentation culture, and the photo-light fermentation culture.
  • a method for expanding health food processed by Cordyceps culture material discloses a puffed health food processed by Cordyceps militaris (C. sinensis) culture material, characterized in that: it is waste after harvesting Cordyceps militaris
  • the culture material is a raw material, and is processed into a puffed health food and a puffed health-care rice slice which are processed by extruding and extruding after being processed, loosely, dried, pulverized, and mixed with water. 200310107651.
  • the whole grain wheat flour is obtained, and the dough is fully mixed and made into a billet, and the billet is sent to a puffing machine for puffing.
  • the puffed semi-finished product is baked in the oven, and the finished product is packaged and stored in the air.
  • the puffing treatment process of edible fungus culture material with branches and crop straw as the main material involving an edible fungus with branches and crop straw as main materials
  • the process of the puffing treatment of the culture material is as follows: (The branches and crop straws are pulverized into chips or granules or segments; (2) The ingredients are mixed according to the following weight parts and stirred evenly: Ingredients: including the pulverized branches Or / and crop straw 75-85; organic materials: 15 -25; inorganic materials: 2-4; (3) puffing treatment: The above-mentioned materials are put into a puffing tank for puffing, the puffing temperature is: 170 -210 ° C, the puffing pressure is: 1.2
  • the culture material of the invention is different from the present invention.
  • the invention enters the drying process after puffing, and the present invention does not require an intrusion procedure.
  • the strain of the invention is different from the present invention.
  • the beneficial effects of the invention are that the source of the raw material is widely stable, and the cultivation process of the edible fungus can be simplified, and the bioconversion rate of the edible fungus can be improved.
  • the literature After examining the literature, there is no "production method of Cordyceps militaris (C. variabilis:) using grain as a medium for expansion), expansion and detoxification, and energy-saving, low-carbon, and shortened production techniques. Therefore, the following new invention methods are proposed.
  • the object of the present invention is to provide a method for puffing rice, wheat, corn, glutinous rice, millet, and yellow rice as main raw materials, and industrializing energy-saving and low-carbon cultivation of Cordyceps militaris.
  • the medium containing wheat, corn, rice, glutinous rice, millet, yellow rice and other foods as the main raw material is rapidly heated and disinfected by puffing treatment to achieve energy-saving and low-carbon, shorten production time, and cultivate the method of Cordyceps militaris.
  • Puffing treatment is the key to the technology of the present invention, and the generally introduced methods include continuous extrusion and air-expansion techniques:
  • Continuous extruder It has compression, mixing, mixing, melting, puffing, molding And other functions.
  • the cavity of the extruder can be divided into 3-5 zones, each zone can be heated by steam or electric, or can be heated by extrusion friction, and the material is gelatinized and denatured by starch under the action of high temperature and high pressure in the cavity.
  • the molten material is extruded through the die hole of the die plate under the action of high pressure. Due to the sudden drop of pressure, the water vapor rapidly expands and dissipates, causing the product to form a porous structure, and then the puffed material is The rotary knife cuts into a certain size product.
  • extrusion is the key to the technology in production. From the structure: divided into one shaft or two shafts, called single-screw extruder and twin-screw extruder; single-screw is divided into left-handed and right-handed, and the double-screw has the same direction and the opposite direction.
  • the left-handed single-screw or right-handed single-screw has a consistent change in the properties of the material in the barrel, while the same direction of the double or anomalous flat 3 ⁇ 4 has a significant difference in the shear strength and cleaning ability of the material.
  • the most common are right-handed single-screw and co-directional double-double-screws. From the working mode; according to whether the material enters the extruder barrel (expansion chamber) before steaming and quenching, it is divided into dry extruder and wet extruder. If it is tempered by steam, it is a wet working mode; otherwise, it is a thousand method.
  • the purpose of steam quenching and tempering to raise the temperature of the material, soften and partially pre-mature the material, and add some water.
  • wet puffing is more efficient than dry puffing. Therefore, less mechanical energy is required, and more energy can be used to increase production.
  • the wear on the screw and barrel is also reduced due to the softening of the material. Due to the thousands/wet method, the properties of the materials entering the barrel are different during production.
  • the wet method into the barrel is a hot and humid material, and the dry method is cold and hard. Material, therefore, the configuration requirements for the spindle system and the extrusion screw are also different. In other words, the dry machine can be produced wet, while the wet machine is not necessarily dry.
  • air puffing technology The initial air puffing equipment is on the street popcorn machine, which is a well-known small business. Every time I process 2 kg of rice, corn or soybeans, children like it very much. At present, air-expansion equipment has been developed, usually with an hourly output: 25-50 kg. There is also a dual air-expansion system with a large output of up to 300 500 kg / h, and has obvious energy-saving effect, a skilled worker can easily operate.
  • the air purifier can puff rice, wheat, corn, buckwheat, beans and other grains to maximize the original flavor, color and nutrients of the grain. Compared with traditional airflow bonds: easy to operate; lead-free puffing; Fast speed; good working performance, stable quality of the products produced.
  • the product After the air is puffed, the product can maintain the shape of the original material well.
  • the product volume after puffing is ten times the volume of the original material.
  • the starch in the raw material is essentially 100% ripened.
  • the culture material after puffing is suitable for the growth of Cordyceps militaris, and it can also be industrialized and automated. Thereby, fresh primordia and fruiting bodies, dried primordia or fruiting bodies, and 100% edible Cordyceps sinensis (: Cordyceps militaris) mycelium (bacteria, bacterium) are obtained.
  • the invention has a strong applicability and a wide range of applications.
  • the residual medium (bacteria, slag) of Cordyceps militaris can directly become a raw material for human food, animal feed, or a raw material for the production of soy sauce, vinegar, and pharmaceutical products, so that no waste is produced. It provides a new way for the sustainable development of the Cordyceps militaris (Ceramicid:) industry.
  • Industrialized energy-saving and low-carbon cultivation method of Cordyceps militaris the invention is characterized by: Rice, wheat and corn are used as the medium, vitamins and inorganic salts are used as additives, and the temperature is sterilized by puffing treatment. The extrusion granulation is carried out, and the liquid strain of Cordyceps militaris is inoculated, mixed and molded, and placed in a container, according to known cultivation.
  • the method of technology management the method can be industrialized and automated production.
  • the invention is characterized in that: rice, wheat, corn, millet, yellow rice, barley, buckwheat, barley, glutinous rice are used as the medium, and the total content of various foods is 97 100%.
  • the medium is made from wheat, corn, rice, millet, yellow rice, barley, buckwheat, barley, glutinous rice, etc.
  • Rice accounts for 5-95%, wheat accounts for 5-50%, and corn accounts for 0-15%.
  • Sorghum rice accounts for 0-30%; yellow rice, barley, buckwheat, barley can also be used as a component of the medium in a ratio of 0-30%; other foods in the raw material range from 0-10% of bran and rice bran.
  • the sum of the ingredients of various foods should be between 97 and 100%.
  • Nationally approved medicinal and food-based products such as Codonopsis pilosula, Astragalus and kelp, can be added to the raw materials as needed to improve health benefits.
  • the invention is characterized in that: the protein, the vitamin and the inorganic salt are used as additives, and the ratio is 0 - 3%.
  • Calcium can regulate the acidity and alkalinity of the medium and increase the calcium concentration of the product.
  • Specific culture method, according to the protein can use 0 - 3.0% ratio of bean cake, peanut bean cake, rapeseed cake, sunflower cake and milk powder, soy milk, egg ingredients; or 0 - 3. 0% of silkworm, silkworm cocoon and silkworm, Silkworm cocoon.
  • the invention is characterized in that: the temperature is sterilized by the expansion treatment, and the medium raw material is formed into a porous structure, the temperature of the expansion is 150 to 210 ° C, and the optimum temperature is 150-170 ° C.
  • the simplest puffing technique is a method of screening, washing, drying, and then frying it into fried rice. Production methods using a continuous process are: continuous extrusion puffing, air puffing technology.
  • A. Continuous extruder It has the functions of compression, mixing, mixing, melting, puffing and molding. The product is formed into a porous structure, and then the expanded material is cut into a certain size by a rotary knife. Small product.
  • the most commonly used in production are single screw extruders and twin screw extruders. In this way, the temperature of the three cylinders is raised from 80 ° C to 140 ° C in a short time, and some of the expansion cylinders have a maximum temperature of 150 to 210 ° C. Therefore, high temperatures can destroy bacteria in the medium.
  • starch gelatinization and protein denaturation can be better utilized by Cordyceps militaris. Therefore, extrusion is the key to the technology in production.
  • the wear on the mast and the barrel is also reduced. Due to the thousands/wet method, the properties of the materials entering the barrel are different during production.
  • the wet method enters the barrel is a hot and humid soft material.
  • the thousand method enters the barrel is a cold and hard material. Therefore, the configuration requirements for the spindle system and the extrusion screw are also different. In other words, the thousand machine can be produced wet, and the wet machine is not necessarily dry. Both the dry and wet machines of the present invention can be used; the optimum temperature for extrusion extruder production is 150-170 Torr.
  • the airflow puffing working range is 150-200 ° C, and the process of the invention does not require 200 ° C high temperature, and can achieve energy saving and low carbon.
  • the optimum temperature for the technique of the present invention is 150-170 Torr.
  • the invention is characterized in that: extrusion granulation is carried out, and the extruded material has a grain shape, a square shape, a circular shape, a rectangular shape, a rhombus shape and other geometric shapes or a puffing and maintaining the original shape of the grain.
  • the purpose of the present invention to form a shape is to allow the culture material to be thoroughly mixed and shaped with the liquid Cordyceps militaris.
  • the cultured materials such as wheat and corn are maintained in the original shape by the air-expansion technique, and the granular wheat and corn culture materials can be thoroughly mixed with the liquid Cordyceps species.
  • the granules after extrusion molding are beneficial to the growth and development of Cordyceps militaris.
  • the present invention is characterized in that the cooling process after the puffing treatment is such that the temperature reaches 25 or less.
  • the temperature of the wheat and rice culture materials after the expansion technology is relatively high. Liquid Cordyceps species is a living life and is not suitable for temperatures above 25 °C, so a cooling process is required. Keep the temperature below 25 °C.
  • the material of the 3 ⁇ 4 screw extrusion extruder is 8-10 seconds in the high temperature pipeline to achieve the disinfection effect. Its water cooling system reduces the material temperature to around 85 °C, which is beneficial for production applications under GMP conditions.
  • the present invention uses a wind cooling technique, or a natural cooling technique, to bring the temperature to 25 ° C or lower and then inoculate the Cordyceps militaris.
  • the method for obtaining the liquid Cordyceps militaris (Stag beetle:) strain is:
  • the liquid Cordyceps militaris (C. cordyceps:) strain is produced according to a known conventional method, and may be added with a vitamin, an inorganic salt additive, and a water-constituting liquid (through high pressure). After sterilization treatment). It is also possible to use a solid Cordyceps militaris (S.
  • the liquid can be replenished with sterile water or physiological saline. Sterile water and physiological saline can be obtained by ordinary sterilization methods or filtration techniques.
  • the invention is characterized in that: the culture material and the liquid Cordyceps militaris (C. sinensis) strain are mixed into a square shape, a barrel shape, a rectangular shape, a rhombus shape and other geometric shapes, and are loaded into the container. Forming methods are either manual or semi-mechanical filling, or production using a line. The purpose of making a shape is to allow the culture material to be thoroughly mixed with the liquid Cordyceps militaris (C. sinensis) strain, usually by electric stirring in a stirred tank.
  • the container is a plastic bag (box, bottle, stick, box) container, a paper material container, a paper plastic recyclable material container, a glass container for manual production, or a semi-mechanical filling, or a production line.
  • the invention is characterized in that: after the completion of the culture material and the stirring tank, the sleeve molding process for the production line in the molding production process is: the container first enters the trough, refills, rolls forming, troughing, capping, racking, cultivating , out of the grass.
  • the method of using compression molding is: After the seed is mixed, after The mold is molded into pieces, then placed in a container, capped, racked, incubated, and grassed.
  • the former container is first placed in the tank, and then filled and capped; the latter is pressed into a block and then placed in a container and capped.
  • a container can be bagged, and a mixture of the culture material and the Cordyceps militaris strain can be grown in the bag. After maturing, the customer takes the finished product in the bag.
  • the role of the container is to maintain growth space, easy to sell and transport in the supermarket.
  • the container can be reused to achieve energy-saving and low-carbon effects. At this time, there is a certain growth space in the container. It is also possible to leave no growth space in the container. After the mature fruit body has grown out of the surface, it is covered with another plastic bag (box, bottle, box) container, paper material container, paper plastic recyclable material container or glass container.
  • Automated production management can be achieved by: preparation, expansion process, cooling, inoculation, sub-assembly, transport to the culture room, culture room temperature, humidity, illuminance management, harvesting process, can be mechanized or automated production.
  • the present invention is characterized in that: the Cordyceps militaris involved, the species that have been preserved in the National Strain Center (the species deposited in the Center of the Species before the patent application date): Cordyceps militaris (Cornrum chinensis), made with the above strains New techniques and methods for inoculating liquid bacteria in expanded food cultures. The method of liquid bacteria has been described above.
  • the method of "industrialized energy-saving and low-carbon cultivation of Cordyceps militaris” is characterized by: 1.
  • 3, can cultivate Ganoderma lucidum on the assembly line, in order to reduce costs, implement automated production, opened up a broad prospect; 4 can The realization of the whole process quality control of Cordyceps militaris and active ingredient processing has laid a solid foundation for Chinese medicine products to enter the international market.
  • Example 1 24 kg of wheat, 75 kg of rice, and then added 0.3 kg of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5 kg of calcium carbonate mixed, after 1 50-160 ⁇ extrusion , to obtain rice grain culture material.
  • the material of the double mast extrusion extruder is 8-10 seconds in the high temperature pipeline to achieve the disinfection effect. Its water cooling system reduces the temperature of the material to around 85 °C. Cool under GMP conditions, bring the temperature to below 25 °C, mix well with liquid Cordyceps sinensis, fill the plastic water container and glass bottle container with 100: 160 water, add 0.5 kg mixed culture to each container. Material, seal.
  • the fruiting body is obtained, and the fresh Cordyceps militaris (Cornrum) can also be collected.
  • the medium part is the edible Cordyceps sinensis (ie, bacillus, slag).
  • Example 2 Using 97 kg of rice, 3 kg of silkworm cocoon powder, and then adding 0.3 kg of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5 kg of calcium carbonate mixed, after 150-160 ° C airflow Puffing to obtain a culture material of the original shape. Cool to inoculate the temperature below 25 °C. It is well mixed with the liquid Cordyceps militaris strain, and the liquid strain contains added vitamin B and yeast. The ratio of material to water is 100: 200, mechanically filled in a plastic container through a production line, and 0.22 kg of mixed culture material is added to each square container to seal.
  • the growth space is not retained in the container, and after the mycelium grows over the surface, it can be transported to another city over a long distance.
  • Example 3 80 kg of wheat, 20 kg of rice, and then added 0.3 g of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5 kg of calcium carbonate and 0.6 g of trace element selenium. After mixing, the mixture was extruded by a twin-screw extruder to obtain a pellet-shaped culture material. The extruder can use cooling water at the same time, lower the temperature to about 85 °C, and then use airflow to cool, and the temperature is below 25 °C. It is thoroughly mixed with the liquid Cordyceps militaris (S. cordyceps) strain, and the ratio of material to water is 100: 150.
  • S. cordyceps Cordyceps
  • the molding process in the molding production process is as follows: The container first enters the trough, refills, rolls, forms, and is covered. Add 0.5 kg of mixed culture material to each container, seal, rack, grow, and graze. For sale in the supermarket, the container can be bagged, and the mixture of the culture material and the Cordyceps militaris species grows in the bag. After maturing, the customer takes the finished product in the bag.
  • a granular culture material was obtained through a gas expansion device 1 50-1. Cool to inoculate the temperature below 25 °C. 0.3 g of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, added to the yeast 0.5 kg, mixed and dissolved in 10 kg of water, autoclaved, and liquid Cordyceps (Cornrum:) Mix the strains, stir the strains, and mix well to make the ratio of water to 100: 180. Liquid Cordyceps species and inorganic salts, vitamin water is not enough 180 kg, can be supplemented with sterile water. Sterile water can be obtained by conventional sterilization methods or filtration techniques.
  • the containers were filled in a square plastic bottle container, and 1 kg of the mixed culture material was added to each container.
  • the method of using compression molding is as follows: After the seed is mixed, it is pressed into a block, then placed in a container, capped, put on, and incubated, according to Known cultivation technology management.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

说 明 书
培育北虫草的方法
技术领域:
本发明属于涉及农业技术和中医药技术领域, 特别是食用、药用 真菌培养和加工的技术领域。
背景技术:
食用药用真菌与人类健康有密切关系, 例如:灵芝、银耳、猴头、 香菇和虫草等几十种真菌。 2010年 3 月进行了检索, 结果说明: 中 国专利局收到关于虫草的发明专利申请就有 927 项之多。 虫草是一 类虫生真菌, 寄生于幼虫, 地球上约 400种, 各种虫草选择不同幼虫 寄生。最早作为药材的中华虫草寄生于蝙蝠蛾幼虫,产地限于高海拔、 高寒地带, 即我国的青海、 西藏、 甘肃及四川。 上世纪 50年代, 加 拿大科学家 Cunmgham经过深入研究, 首先发现北虫草具有更高的 医药价值。 北虫草又称北冬虫夏草、 东北冬虫夏草、 蛹虫草、 蛹草, 寄生于蛹体。 自 20 世纪 90 年代开始, 北虫草 (蛹虫草)人工栽培方 法在生产中需用蒸煮消毒灭菌的方法进行前处理,这个方法消耗热能 大, 时间长。 100°C, 常压 8 10小^ ; 127 , 1公斤的高压还需要 2小时。 特别是玻璃瓶、 塑料瓶之类的包装容器也一同升高温度。 所 以, 消耗热能大, 生产时间长。 本发明 "工业化节能低碳培育北虫草 的方法" 涉及食用、 药用真菌培养方法, 通过膨化处理培养基原料 的关键技术, 达到节能低碳、 自动化生产的工艺。 经过文献比较: 200410059964.X蛹虫草食品和誦虫草基质及其制备方法。它采用 蛹虫草基质与熟大米粉、 牛扔、 植物油、 蔗糖等配料制作的蛹虫草食 品。 所述配料为米粉晶类食品或膨化类食品常用的配料, 木醇糖、 卵 磷脂、 柠檬酸、 碳酸氢销、 香兰素、 起酥油、 盐、 抗氧剂。 螭虫草菌 种可以自行制备或选购, 誦虫草基质选择优质东北大米、 土豆、 胡萝 卜经煮沸, 高 灭菌后装到料瓶中备用, 在制备发酵营养液, 将蛹虫 草液体菌种混合到营养液中接种到料瓶中;经避光发酵培养和散光发 酵培养及补光发酵培养后进行采收, 可制备成米粉晶或米果。
200710004106. 9 名称:一种虫草培养料加工的膨化保健食品的方法, 公开了一种北虫草 (蛹虫草:)培养料加工的膨化保健食品, 其特征是: 它是利用采摘北虫草后的废弃培养料为原料, 经加工松碎、 干燥、 粉 碎、加水混合后经挤压膨化设备加工制成的膨化保健食品及膨化保健 米片。 200310107651. 2 名称: 膨化灵芝孢子粉制品及其制作方法 , 将灵芝孢子粉进行浸泡漂洗, 经干燥后在 126〜160°C温度下进行膨 化处理, 膨化后进行研磨至 20〜300 目, 包装制成产品。 200610067086. 5 名称: 富硒膨化食品的加工方法, 公开了一种富硒 膨化食品的加工方法。 200710060421. 3的专利名称: 小麦膨化食品 及制作方法是全粮小麦膨化食品及制作方法。 由全原粮小麦、 薏米、 黄豆、 绿豆、 茯苓、 远志、 核桃仁、 黑芝麻、 调味料组成, 获得全粮 小麦粉,加入和面机充分拌和制成坯料,把料坯送入膨化机进行膨化, 膨化后的半成品在烤箱烘烤, 晾凉后成品包装入库。 <申请号 >200510012814 <发明名称>以枝条、 农作物秸秆为主料的食用菌培 养料的膨化处理工艺, 涉及一种以枝条、农作物秸秆为主料的食用菌 培养料的膨化处理工艺, 其工艺歩骤如下: ( 将枝条、 农作物秸秆 粉碎成屑状或颗粒状或段状; (2)按下列重量份配料并搅拌均匀: 主 料:包括粉碎后的枝条或 /和农作物秸秆 75-85;有机物辅料: 15 -25; 无机物辅料: 2-4; (3)膨化处理: 将上述配好的物料装入膨化罐中进 行膨化处理, 膨化温度为: 170-210°C, 膨化压力为: 1.2-2.0Mpa, 膨化时间为: 10-15 分钟; 之后瞬间喷爆入仓; (4)烘干: 将膨化后 的物料烘干至含水量为 13-15% ,即得食用菌培养料。经过比较看到:
1、 垓发明的培养原料与本发明不同。 2、该发明在膨化以后进入烘干 工序, 本发明进入不需要迸入程序。 3 该发明的菌种与本发明不同。
本发明的有益效果是原料来源广泛稳定,能够简化食用菌栽培工 艺,提高食用菌生物转化率。经过检査文献,没有和本发明重叠的 "生 产北虫草 (螭虫草:)利用粮食为培养基进行膨化的方法" , 膨化升温消 毒, 达到节能低碳、 缩短生产工艺的培养技术。 所以, 提出以下新的 发明方法 。
发明内容:
本发明的目的: 提供一种大米、 小麦、 玉米、 高粱米、 小米、 黄 米粮食类成分为主要原料进行膨化处理,工业化节能低碳培育北虫草 (蛹虫草)的方法。 以小麦、 玉米、 大米、 高粱米、 小米、 黄米等粮食 为主要原料的培养基, 通过膨化处理,快速升温消毒, 达到节能低碳、 缩短生产时间,培育北虫草 (誦虫草)的方法。膨化处理是本发明的技术 关键, 一般介绍的方法有连续挤压膨化和气流膨化技术:
1、 连续挤压机: 它具有压缩、 混合、 混炼、 熔融、 膨化、 成型 等功能。挤压机的腔体可以分成 3-5个区, 各区可以通过蒸汽或电加 热, 也可通过挤压摩擦加热, 物料在腔体中高温、 高压的作用下, 淀 粉糊化、 蛋白质变性。在挤压腔体的末端, 熔融的物料通过在高压的 作用下通过模板的模孔而挤出, 由于压力的突然下降, 水蒸汽迅速膨 胀和散失, 使产品形成多孔结构, 然后膨化的物料被旋转刀切成一定 大小的产品。 在生产中最常使用的是单螺杆挤压机和双螺杆挤压机。 这种方法三个筒的温度在短时间从 升高到 160 ,有的 膨化筒最高温度在 150〜190°C之间。 所以, 高温可以消灭培养基中 的细菌。 另外, 淀粉糊化和蛋白质变性以后能被北虫草 (蛹虫草)菌更 好地利用。 所以, 挤压膨化是生产中的技术关键。 从结构上: 分一根 轴或两根轴, 称为单螺杆膨化机及双螺杆膨化机; 单螺杆又分为左旋 右旋, 双螺 又有同向平双和异向平双之分。 左旋单螺杆或右旋单螺 杆对物料在机筒内的性状改变基本是一致的,而同向平双或异向平¾ 对物料的剪切强度和清理能力是有显著差别的。最为常见的是右旋单 螺杆和同向平双双螺杆。 从工作方式上; 按照物料进入膨化机机筒 (膨化腔)前是否经蒸汽调质分为干法膨化机和湿法膨化机。若经蒸 汽调质, 则为湿法工作模式; 反之, 则为千法。 蒸汽调质的目的: 提升料温、 软化及部分予熟化物料、 补充一定水分。 对于绝大多数物 料,湿法膨化都比干法膨化效率高。因此,所需要的机械能较千法少, 更多的能量可以用来提高产量。 此外, 由于物料的软化, 对螺杆、 机 筒的磨损也会降低。 由于千 /湿法两种方法在生产时物料进入机筒的 性状不同。湿法道入机筒是湿热的软物料, 干法迸入机筒是生冷的硬 物料, 因此, 对主轴系及挤压螺旋的配置要求也不同。 换言之, 干法 机可以湿法生产, 而湿法机不一定能干法使用。
2、气流膨化技术: 最初的气流膨化设备是在街头的爆玉米花机, 这是尽人皆知的小生意。每次加工 2斤大米、玉米或者黄豆, 儿童都 十分喜欢。 目前, 发展起来的气流膨化设备, 通常有每小时产量: 25-50公斤。 还有双气流膨化系统具有产量大, 可达 300 500公斤/ 小时, 且具有明显的节能效果, 一个熟练工可轻松操作。气流膨化机 可膨化大米、 小麦、 玉米、 荞麦、 豆类等五谷杂粮, 最大限度的保持 了粮食原有的风味、 色泽、 营养成分, 与传统的气流鍵相比较: 操作 方便; 无铅膨化; 速度快; 工作性能好, 生产出来的产品质量稳定。 气流膨化后产品能很好地保持原来物料的外形。 气流膨化后的产品 体积是原物料体积的十倍。 原料中的淀粉基本上是 100%的熟化。 膨化以后的培养原料适合北虫草 (鋪虫草)生长, 也能够进行工业 化生产和自动化管理。从而, 得到新鲜的原基和子实体, 干燥的原基 或子实体, 以及 100%可食性的北虫草 (:蛹虫草:)菌丝体(菌糠、 即菌 渣) 。 本发明的实用性强、 可应用的范围广。 特别是北虫草 (蝻虫草) 的残余培养基(菌糠、 菌渣) , 能够直接成为人类食品的原料、 动物 饲料或者成为生产酱油、醋和医药产品的原料,从而,没有废物产生。 为北虫草 (蛹虫草:)产业的可持续性发展提供了新途径。
下面叙述方法和歩骤:
1、 工业化节能低碳培育北虫草的方法, 本发明的特征是: 以大 米、 小麦、 玉米为培养基, 以维生素和无机盐为添加剂, 通过膨化处 理实现升温消毒, 实现挤压造粒, 接种北虫草液体菌种, 混合成型, 装入容器内, 根据已知的栽培技术进行管理的方法, 本方法可以产业 化、 自动化生产。
2、 本发明的特征是: 以大米、 小麦、 玉米、 小米、 黄米、 青稞、 荞麦、 大麦、 高粱米粮食类成分为为培养基, 各种粮食类的成分总和 在 97 100 %。 所谓培养基是以小麦、 玉米、 大米、 小米、 黄米、 青稞、 荞麦、 大麦、 高粱米等粮食为原料, 大米占 5-95%, 小麦占 5-50 %, 玉米占 0-15%, 高粱米占 0-30 %; 黄米、 青稞、 荞麦、 大麦 也可以使用 0-30 %的比例, 成为培养基的成分; 原料中的其他粮食 类的麸皮和米糠占 0-10 %。 但是, 各种粮食类的成分总和应该在 97- 100 %。根据需要可以在原料中加入国家批准的药用和食品两用 的产品, 例如党参、 黄芪以及海带类成分, 这样可以提高保健效果。
3、 本发明的特征是: 以蛋白质、 维生素和无机盐为添加剂, 比例为 0 - 3 %。 碳酸钙、 乳酸钙、 硫酸钙、 柠檬酸钙为调节剂, 比 例为 0 - 1 %。钙可以调节培养基的酸碱性和增加产品的钙浓度。具 体培养方法, 根据蛋白质多少可以使用 0 - 3.0 %比例的豆饼、 花生 豆饼、 菜籽饼、 葵花子饼以及牛奶粉、 豆浆、 鸡蛋成分; 或者 0 - 3. 0 %比例的蚕、蚕蛹和柞蚕、 柞蚕蛹。 也可以添加 0 - 1.0 % 比例的 氮肥 (柠檬酸铵以及其他含氮物质); 添加 0- 0.1 %左右比例的钾 盐, 0-0.05 % 比例左右的硫酸镁。 硒是人体重要的微量元素, 世界 卫生组织建议每天补充 200 硒, 可有效预防多种疾病的高 发。比较支持采用安全性更高的含硒蛋白、氨基酸等有机形态硒, 或富含硒的农副产品。 目前, 亚硒酸钠片每片含亚硒酸钠 1 m g, (相当于含硒 457 g)11 岁以上及成人每次 2片。 服用过量 硒可引起中毒, 每日最大安全量为 400 u g D 所以, 参考这个数 据在培养基中按照 0 _0. 8mg/kg的比例添加微量元素硒。 使得成品 中达到每 100克北虫草 (螭虫草)含硒 20 - 50 μ g。 维生素在膨化 过程中会有损失, 所以, 通常加入少量的维生素 B; 或者, 加入酵母 的比例为 0-0.5%, 酵母含有大量的维生素 B族的成分。 因为微量元 素和维生素在原料中有差别, 所以, 在用量上从 0% 到 0.5% 不等, 要根据原料来源进行考虑。这些蛋白质、维生素和无机盐的添加剂在 生产过程中有两种加入方法: 一、 把蛋白质、 维生素和无机盐的添加 剂粉碎以后和粮食类原料充分混合, 然后进入膨化处理, 实现消毒作 用; 二、 把蛋白质、 维生素和无机盐的添加剂粉碎后, 溶入十倍以上 的水中, 经过高压灭菌处理, 和液体北虫草 (誦虫草:)菌种混合, 在搅 拌工艺过程中使用。
4、 本发明的特征是: 通过膨化处理实现升温消毒, 使培养基原 料形成多孔结构,膨化生产的温度 150 ~ 210°C ,最佳温度是 150-170 °C。 最简单的膨化技术是筛选、 清洗、 烘干, 再将其炒制膨化成为炒 米花的方法。 使用连续工艺的生产方法有: 连续挤压膨化、气流膨化 技术。
A、 连续挤压机: 它具有压缩、 混合、 混炼、 熔融、 膨化、 成型 等功能。使产品形成多孔结构, 然后膨化的物料被旋转刀切成一定大 小的产品。在生产中最常使用的是单螺杆挤压机和双螺杆挤压机。这 种方法三个筒的温度在短时间从 80°C 140°C升高到 160°C,有的膨 化筒最高温度在 150〜210°C。 所以, 高温可以消灭培养基中的细菌。 另外, 淀粉糊化和蛋白质变性以后能被北虫草 (蛹虫草)菌更好地利 用。 所以, 挤压膨化是生产中的技术关键。 从结构上; 分一根轴或两 根轴, 称为单螺杆膨化机及双螺杆膨化机; 最为常见的是右旋单螺杆 和同向平¾双螺杆。 从工作方式上: 按照物料进入膨化机机筒(膨化 腔)前是否经蒸汽调质分为千法膨化机和湿法膨化机。若经蒸汽调质, 则为湿法工作模式; 反之, 则为干法。 蒸汽调质的目的; 提升料温、 软化及部分予熟化物料、 补充一定水分。对于绝大多数物料 湿法膨 化比千法膨化效率高。 因此, 所需要的机械能较千法少, 更多的能量 可以用来提高产量。 外, 由于物料的软化, 对嫘杆、 机筒的磨损也 会降低。 由于千 /湿法两种方法在生产时物料进入机筒的性状不同。 湿法进入机筒是湿热的软物料,千法进入机筒是生冷的硬物料,因此, 对主轴系及挤压螺旋的配置要求也不同。换言之, 千法机可以湿法生 产, 而湿法机不一定能干法使用。本发明技术干法机和湿法机都可以 使用; 挤压膨化机生产的最佳温度是 150- 170Ό。
Β、 气流膨化技术: 目前, 发展起来的气流膨化设备, 每小时产 量在 25-50公斤。 还有双气流膨化系统产量更大, 可达 300- 500公 斤 /小 , 与传统方式机型相比节能达 1/3。 罐内温度达到 200°C, 粮 食原料体积增加 10倍。 温度越高, 膨胀体积越大。 在本发明生产中 原料体积膨化增大, 不是最终的目的和效果。 需要的是消灭细菌, 适 当膨胀体积 3-5倍就达到目的。 现在, 新设备使用了温度传感系统, 可以即时反映罐内的正确温度。由于 150- 180°C在短暂的几秒内就能 够杀死微生物。所以, 生产中根据温度指示、 只要达到杀菌要求就可 以了。气流膨化工作范围 150- 200°C,本发明工艺不需要 200°C高温, 可以达到节能低碳。 本发明技术最佳温度是 150- 170Ό。
5、 本发明的特征是: 实现挤压造粒, 挤压膨化的物料有米粒形、 方形、 园形 、 长方形 、 菱形以及其它几何形状或者气流膨化保持粮 食颗粒的原形。本发明形成一定形状的目的是让培养料能够和液体北 虫草 (蛹虫草:)菌种充分混合和造型。 气流膨化技术处理以后的培养 料, 如小麦、 玉米都保持原来的形状, 颗粒状的小麦、 玉米培养料能 够和液体北虫草菌种充分混合。挤压造型以后的颗粒有利于北虫草菌 的生长、 发育。
6、 本发明的特征是: 膨化处理以后的冷却工艺, 使温度达到 25 以下。膨化技术处理以后的小麦、 大米培养料的温度比较高。液体 北虫草菌种是活的生命, 不适合 25 °C以上的温度, 所以, 需要采取 冷却工艺。 使温度达到 25 °C以下。 ¾螺杆挤压膨化机的物料在高温 的管道中有 8-10秒的时间, 达到消毒效果。 它的水冷却系统, 可以 使物料温度降低到 85 °C左右, 有利于在 GMP 条件下生产应用。 本发明使用风冷却技术, 或者使用自然冷却技术, 使温度达到 25 °C 以下再进行北虫草菌接种的工艺。
7、 本发明的特征是: 接种液体北虫草 (螭虫草:)菌种, 培养料和 液体的比例是 100: 100-500, 最合适的范围是: 培养料: 液体 = 100: 110 - 300。获得液体北虫草 (螭虫草:)菌种的方法是: 液体北虫草 (蛹虫 草:)菌种按照已知的常规方法生产, 使用时可以加入维生素、 无机盐 添加剂和水组成的液体 (经过高压灭菌处理后)。 还可以利用固体北 虫草 (麵虫草:)菌种在 GMP条件下或者是无菌室的无菌要求下, 加入 2-5倍体积的无菌液体(水), 用搅拌机、匀浆机打碎成为液体北虫草 (爾虫草)菌种。 用液体北虫草 (插虫草)菌种和膨化后的培养料拌和均 匀。 以往的培养料经过高压灭菌或者 8小时蒸煮以后是潮湿的, 含水 量在 80-150%。经过膨化的培养料含水量在 6-18 %, 本发明方法的培 养料和液体的比例是 100: 100-500。最合适的范围是培养料和液体的 比例是 100: 110 - 300。 所以, 本方法使用液体的数量比较大; 这与 过去传统的方法有完全的不同。 液体可以用无菌水或者生理盐水补 充。 无菌水、 生理盐水可以用普通的消毒方法或者过滤的技术获得。
8、 本发明的特征是: 培养料和液体北虫草 (誦虫草:)菌种混合成 方形、 园桶形 、 长方形 、 菱形以及其它几何形状, 装入容器内。 成 型的方法有手工或者半机械充填, 或者用流水线生产。制造成一定形 状的目的是: 让培养料能够和液体北虫草 (螭虫草:)菌种充分混合, 通 常用电动搅拌的方法在搅拌槽内完成。 按照常规方法容器是塑料袋 (箱、 瓶、 棒、 盒)容器, 纸质材料容器、 纸塑可回收材料容器、 玻 璃容器内进行手工生产, 或者半机械充填, 或者用流水线生产。本发 明的特征是: 培养料和搅拌槽内完成以后, 成型生产工序中流水线生 产的套模歩骤是: 容器先入套槽, 再灌料, 滚压成型, 出槽, 加盖, 上架, 培育, 出草。 或者使用压模成型的方法是: 料种混合后, 经过 压模成块, 再装入容器, 加盖, 上架, 培育, 出草。前者容器先入槽, 然后灌料加盖; 后者料种压模成块, 再装入容器, 加盖。 为了在超级 市场上销售, 可以在容器上套袋, 培养料和北虫草 (蛹虫草)菌种的混 合物在袋内生长, 成熟以后, 顾客拿走袋内成品。容器的作用是保持 生长空间、 超级市场上销售和运输方便。 在这里容器可以重复使用, 能够达到节能低碳的效果。 这时, 容器中有一定生长空间。 还可以在 容器中不保留生长空间, 成熟的子实体长出表面以后, 用另外的塑料 袋(箱、 瓶、 盒)容器, 纸质材料容器、 纸塑可回收材料容器或玻璃 容器套上。
9、 经过以上歩骤就可以根据已知的栽培技术进行管理, 本方法 可以自动化生产管理。 自动化生产管理表现在: 备料, 膨化过程, 降 温, 接种, 分装成型, 运到培养室, 培养室内的温度、 湿度、 光照度 管理, 收割过程, 都可以实现机械化或者自动化生产。
10、 本发明的特征是: 所涉及的北虫草, 以国家菌种中心已经 保藏的品种 (专利申请日以前在菌种中心保藏的菌种) : 北虫草 (蛹 虫草), 用以上菌种制做液体菌种接种在膨化的粮食类培养料中完成 的新技术、 新方法。 液体菌种的方法已经在前面进行叙述。
使用新方法有如下优点:本发明"工业化节能低碳培育北虫草的 方法" , 特点是: 1、 利 ^粮食类原料能够膨化的原理, 快速升温消 毒, 达到节能低碳、 缩短生产时间的目的; 2、 玻璃瓶、 塑料瓶之类 的包装容器不需要升温处理, 可以节能低碳; 3、 能够流水线上培育 灵芝, 为降低成本、 实行自动化生产, 开辟了广阔的前景; 4、 可以 实现北虫草以及有效成分加工的全程质量控制, 为中医药产品走向国 际市场奠定了可靠的基础。
下面通过实例进行说明:
实施例 1 : 使用小麦 24公斤, 大米 75公斤, 再加入 0. 3公 斤 柠檬酸铵, 0 .1 公斤钾盐, 0.05 公斤硫酸镁, 0.5公斤碳酸钙混 合以后, 经过 1 50- 160Ό 挤压膨化, 得到米粒形培养料。 双嫘杆挤 压膨化机的物料在高温的管道中有 8-10秒的时间, 达到消毒效果。 它的水冷却系统, 可以使物料温度降低到 85°C左右。在 GMP条件下 冷却, 使温度达到 25°C以下, 和液体北虫草菌种充分混合, 料水比 100: 160, 在塑料瓶容器和玻璃瓶容器内进行充填, 每个容器加入 0.5公斤混合培养料, 封口。 在培养料的表面被菌丝布满以后, 增加 通气量。 按照已知的栽培技术管理。 从而, 得到子实体、 还可以收 集新鲜北虫草 (蛹虫草)商品。 收集新鲜的子实体以后, 培养基部分是 具有可食性的北虫草菌丝体 (即菌糠、 菌渣) 。
实施例 2: 使用大米 97公斤, 蚕蛹粉 3公斤, 再加入 0. 3公斤 柠檬酸铵, 0 .1 公斤钾盐, 0.05 公斤硫酸镁, 0.5公斤碳酸钙混合以 后, 经过 150- 160°C 气流膨化, 得到原来形状的培养料。 冷却, 使 温度达到 25 °C以下接种。 和液体北虫草菌种充分混合, 液体菌种内 含有添加的维生素 B和酵母。 料水比 100: 200, 经过流水生产线在 塑料容器内进行机械充填, 每个方形容器加入 0.22公斤混合培养料, 封口。在容器中不保留生长空间, 菌丝体长满表面以后, 就可以长途 运输到另外的城市。 用另外的塑料袋(箱、 瓶、 盒)容器, 纸质材料 容器、纸塑可回收材料容器或玻璃容器套上。按照已知的栽培技术管 理, 收获产品。
实施例 3 : 使用小麦 80公斤,大米 20公斤,再加入 0. 3公斤 柠 檬酸铵, 0 .1 公斤钾盐, 0.05 公斤硫酸镁, 0.5 公斤碳酸钙和 0. 6g 的微量元素硒。混合以后, 经过双螺杆膨化机挤压膨化, 得到颗粒形 培养料。 挤压机可以同时使用冷却水, 降低温度到 85 °C左右, 再使 用气流冷却, 温度达到 25 °C以下接种。 和液体北虫草 (;誦虫草)菌种 充分混合, 料水比 100 : 150, 成型生产工序中套模歩骤是: 容器先 进入套槽, 再灌料, 滚压成型, 出槽, 加盖, 每个容器加入 0.5公斤 混合培养料, 封口, 上架, 培育, 出草。 为了在超级市场上销售, 可 以在容器上套袋, 培养料和北虫草菌种的混合物在袋内生长, 成熟以 后, 顾客拿走袋内成品。
实施例 4:
使用小麦 100公斤, 经过气流膨化设备 1 50-1 得到粒形培 养料。冷却, 使温度达到 25 °C以下接种。把 0. 3公斤 柠檬酸铵, 0 .1 公斤钾盐, 0.05 公斤硫酸镁, 加入酵母 0.5公斤, 混合以后溶入十公 斤的水中, 经过高压灭菌处理, 和液体北虫草 (蛹虫草:)菌种混合, 搅 拌菌种, 充分混合, 使料水比 100: 180。 液体北虫草菌种和无机盐、 维生素水不够 180公斤, 可以用无菌水补足。 无菌水可以用普通的 消毒方法或者过滤的技术获得。搅拌以后, 在方形塑料瓶容器内进行 充填, 每个容器加入 1公斤混合培养料。使用压模成型的方法是: 料 种混合后, 经过压模成块, 再装入容器, 加盖, 上架, 培育, 按照已 知的栽培技术管理。
参考文献:
1、 王国栋主编: 冬虫夏草类生态 培植 应用, 科学技术文献出 版社 1995年 8月 第一版 158、 172
2、 李 昊: 虫草人工栽培技术 金盾出版社 2000年 11 月 第一 版 29

Claims

权 利 要 求 书
1. 工业化节能低碳培育北虫草的方法,本发明的特征是: 以小麦、 大米为培养基, 以维生素和无机盐为添加剂, 通过膨化处理实现升温消 毒, 实现挤压造粒, 接种液体北虫草菌种, 混合成型, 装入容器内, 根 据已知的栽培技术进行管理的方法, 本方法可以产业化、 自动化生产。
2.根据权利要求 1 所说的方法,本发明的特征是: 以大米、 小麦、 玉米、 小米、 黄米、 青稞、 荞麦、 大麦、 高粱米粮食类成分为为培养基, 粮食类的成分总和在 97 100 %。
3.根据权利要求 1所说的的方法,其特征是: 以蛋白质、 维生素和 无机盐为添加剂, 比例为 0 - 3 %。
4.根据权利要求 1 所说的的方法,其特征是:通过膨化处理实现升 温消毒, 使培养基原料形成多孔结构, 膨化生产的温度 1 50 - 210Ό, 最佳温度是 150~170°C。
5.根据权利要求 1 所说的的方法,其特征是: 实现挤压造粒, 挤压 膨化的物料有米粒形、 方形、 园形 、 长方形 、 菱形以及其它几何形状 或者气流膨化保持粮食颗粒的原形 。
6.根据权利要求 1 所说的的方法,其特征是:膨化处理以后的冷却 工艺, 使温度达到 25 °C以下。
7.根据权利要求 1所说的的方法,其特征是:接种液体北虫草 (誦虫 草:)菌种, 培养料和液体的比例是 100: 100-500, 最合适的范围是: 培 养料: 液体 = 100: 110 - 300。
8.根据权利要求 1所说的的方法,其特征是: 培养料和液体北虫草 (蛹虫草:)菌种混合成方形、 园桶形 、 长方形 、 菱形以及其它几何形状, 装入容器内。
9.根据权利要求 1所说的的方法,其特征是: 成型流水线生产的套 模或者压模的方法。
10.根据权利要求 1所说的的方法,其特征是:所涉及的北虫草, 以 国家菌种中心已经保藏的品种 (专利申请日以前在菌种中心保藏的菌 种) : 北虫草 (蛹虫草:), 用以上菌种制做液体菌种接种在膨化的粮食类 培养料中完成的新技术、 新方法。
PCT/CN2011/071256 2010-03-22 2011-02-24 培育北虫草的方法 WO2011116649A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201010128996.6 2010-03-22
CN2010101289966A CN101971764A (zh) 2010-03-22 2010-03-22 工业化节能低碳培育北虫草的方法

Publications (1)

Publication Number Publication Date
WO2011116649A1 true WO2011116649A1 (zh) 2011-09-29

Family

ID=43571699

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2011/071256 WO2011116649A1 (zh) 2010-03-22 2011-02-24 培育北虫草的方法

Country Status (2)

Country Link
CN (1) CN101971764A (zh)
WO (1) WO2011116649A1 (zh)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101971764A (zh) * 2010-03-22 2011-02-16 陈顺志 工业化节能低碳培育北虫草的方法
CN101971763A (zh) * 2010-03-22 2011-02-16 陈顺志 工业化节能低碳培育灵芝的方法
CN103329735A (zh) * 2013-07-19 2013-10-02 何寒 一种利用大米作为培养基制作食用菌试管母种的方法
CN104584855A (zh) * 2013-10-31 2015-05-06 江苏学府生物工程有限公司 一种北虫草挤压膨化灭菌的生产方法
CN104584856A (zh) * 2013-10-31 2015-05-06 江苏学府生物工程有限公司 一种北虫草气流膨化灭菌的生产方法
CN103733885B (zh) * 2014-01-13 2016-01-06 王永显 一种北虫草米粉粒的制作方法
CN105638231A (zh) * 2014-11-07 2016-06-08 江苏学府生物工程有限公司 一种北虫草高产的方法
CN104823694A (zh) * 2015-03-20 2015-08-12 江南大学 虫草芭蕉芋
CN104823696A (zh) * 2015-03-20 2015-08-12 江南大学 虫草赤小豆
CN104823692A (zh) * 2015-03-20 2015-08-12 江南大学 虫草小麦
CN104718986A (zh) * 2015-03-20 2015-06-24 江南大学 虫草山药
CN104823695A (zh) * 2015-03-20 2015-08-12 江南大学 虫草花生
CN104718985A (zh) * 2015-03-20 2015-06-24 江南大学 虫草大米
CN104718988A (zh) * 2015-03-20 2015-06-24 江南大学 虫草莲子
CN104823693A (zh) * 2015-03-20 2015-08-12 江南大学 虫草黄豆
CN104686199A (zh) * 2015-03-20 2015-06-10 江南大学 虫草青稞
CN104686937A (zh) * 2015-03-20 2015-06-10 江南大学 虫草西米
CN104823689A (zh) * 2015-03-20 2015-08-12 江南大学 虫草小米
CN105586266B (zh) * 2015-12-25 2019-03-05 江苏大学 一种生产富硒冬虫夏草菌丝原料的方法
CN107929788A (zh) * 2016-10-12 2018-04-20 镇江市天益生物科技有限公司 一种香菇废料灭菌的生产方法
CN107624503B (zh) * 2017-11-03 2022-04-26 鲁东大学 一种虾类饲料添加剂及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692840A (zh) * 2005-06-21 2005-11-09 程宽 以枝条、农作物秸秆为主料的食用菌培养料的膨化处理工艺
CN101463325A (zh) * 2008-12-16 2009-06-24 浙江博士园生物技术有限公司 北冬虫夏草的工厂化栽培方法
CN101971764A (zh) * 2010-03-22 2011-02-16 陈顺志 工业化节能低碳培育北虫草的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082807A (zh) * 1992-08-19 1994-03-02 管代义 北冬虫夏草人工培育的方法
CN100370015C (zh) * 2005-09-30 2008-02-20 邓杰华 冬虫夏草菌或者蛹虫草菌的培养方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692840A (zh) * 2005-06-21 2005-11-09 程宽 以枝条、农作物秸秆为主料的食用菌培养料的膨化处理工艺
CN101463325A (zh) * 2008-12-16 2009-06-24 浙江博士园生物技术有限公司 北冬虫夏草的工厂化栽培方法
CN101971764A (zh) * 2010-03-22 2011-02-16 陈顺志 工业化节能低碳培育北虫草的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BAO, JIANZHONG ET AL: "Study on processing technology of Cordyceps militaris on scale and its demonstration and popularization", MODERN AGRICULTURAL SCIENCES, vol. 16, no. 1, January 2009 (2009-01-01), pages 70 *

Also Published As

Publication number Publication date
CN101971764A (zh) 2011-02-16

Similar Documents

Publication Publication Date Title
WO2011116649A1 (zh) 培育北虫草的方法
WO2011116648A1 (zh) 工业化节能低碳培育灵芝的方法
CN101822170A (zh) 一种基于固态表面培养生产樟芝菌丝体的方法
CN103798057A (zh) 一种银耳培养基及其栽培方法
CN107011072A (zh) 一种猕猴桃专用有机肥及其制备方法
CN105061057A (zh) 高产茭白专用有机肥
CN104106374B (zh) 利用甘蔗渣、桑杆和玉米渣生产姬菇的方法
CN104206169A (zh) 利用蛹虫草培养基制备营养麦片的方法
CN108633625A (zh) 以杏鲍菇菌糠为主要原料制备双孢菇栽培基的方法
CN104429589A (zh) 利用剑麻废渣生产猴头菇的方法
CN104381015A (zh) 利用剑麻废渣生产香菇的方法
CN107821004B (zh) 一种利用艾渣栽培榆黄蘑的培养基及其栽培方法
CN102177812A (zh) 一种草菇增产的方法
CN104975051A (zh) 一种富集桑叶中gaba的方法
CN111436528A (zh) 一种基于微生物发酵的竹粉饲料制备方法
CN103461808A (zh) 一种菌化粮及制备方法
CN106473065A (zh) 纳豆发酵花粉的生产方法
KR20140127390A (ko) 유색팽이버섯 배지 조성물 제조방법 및 이를 이용한 버섯 재배방법
KR100752335B1 (ko) 상황버섯균사체를 이용한 느타리버섯의 재배방법
KR102366791B1 (ko) 산천어 발효액 제조방법
CN109511467A (zh) 一种平菇培养基及其制备方法
CN107619322A (zh) 一种专用于栽培草菇的培养基质及其制作方法
CN101161809B (zh) 一种农副产品发酵用曲的制作方法
CN108373347A (zh) 一种段木食用菌培养基的制作技术
KR100537557B1 (ko) 한약재첨가누룩의제조방법

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11758759

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11758759

Country of ref document: EP

Kind code of ref document: A1