WO2011099665A1 - Composition antimicrobienne contenant des extraits d'ingrédients naturels, antiseptique naturel composite et procédé de fabrication associé - Google Patents

Composition antimicrobienne contenant des extraits d'ingrédients naturels, antiseptique naturel composite et procédé de fabrication associé Download PDF

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WO2011099665A1
WO2011099665A1 PCT/KR2010/000920 KR2010000920W WO2011099665A1 WO 2011099665 A1 WO2011099665 A1 WO 2011099665A1 KR 2010000920 W KR2010000920 W KR 2010000920W WO 2011099665 A1 WO2011099665 A1 WO 2011099665A1
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extract
extracts
complex
antimicrobial composition
antimicrobial
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PCT/KR2010/000920
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English (en)
Korean (ko)
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김동명
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주식회사 케이씨아이
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Publication of WO2011099665A1 publication Critical patent/WO2011099665A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

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  • the present invention relates to an antimicrobial composition and a composite natural preservative comprising the same as an active ingredient, and more particularly, to an antimicrobial composition including a processed extract obtained by enzymatic treatment or fermentation of an extract or extract extracted from a natural raw material selected by bioscreening. And it relates to a composite natural preservative prepared in various forms using the same.
  • preservatives that are not useful to the human body to prevent changes in internal physical properties and to preserve for a certain period of time.
  • the material used as the preservative there are some materials produced in nature, but most of them are chemically synthesized artificial materials.
  • most cosmetics are suitable for the growth of microorganisms and chemicals are susceptible to decay, and artificial preservatives such as cheap parabens, imidazolidinyl urea, diazolidinyl urea, phenoxy ethanol, etc. Is being used.
  • the present invention has been made to solve the conventional problems, one object of the present invention is to reduce the side effects of the conventional synthetic preservatives are safe for the human body, while at the same time broad antibacterial spectrum, high antibacterial activity, formulation stability and reproducibility It is to provide a composite natural preservative having an antimicrobial composition as an active ingredient thereof. Another object of the present invention is to provide a method for preparing the antimicrobial composition and the composite natural preservative.
  • the inventors of the present invention through bioscreening for antimicrobial activity and antioxidant activity against about 2500 kinds of natural products of plant origin, as a candidate material, soybean sprouts, green onions, shiitake mushrooms, tung mushrooms , Ho jang-geun, golden, yellow white, yellow lotus, cedar leaf, broccoli, sancho, triticale, eosungcho, saori, sessin, legitimate, pine needle, knee root, jinjin mugwort, buttercup, ginger, poisonous, yacon, omija, indongcho, grapefruit, jangsaeng Bellflower, Angel, Red pepper, plantain, Perilla, Sesame, Molewood, Let's go, Licorice, River, Ginseng, Seokgok, Coriander, Spectator, Gukje, Gujeolcho, Cudrania, Geuminja, Geumakja, Gilkyung, Mandang, Dansam, Dangyuja,
  • the present invention is the extract of ⁇ , bean sprouts extract, green onion extract, shiitake mushroom extract, chopped mushroom extract, kojang-gun extract, golden extract, baekbaek extract, sulfur extract, cedar leaf extract, brokerage extract, Sancho extract, Triticale extract, Echo extract, Prunus extract, Secinin extract, Root extract, Pine needle extract, Knee root extract, Injin mugwort extract, Buttercup extract, Ginger extract, Poison extract, Yacon extract, Schisandra extract, Indongcho extract, Grapefruit extract, One or more extracts selected from the group consisting of Jangsaeng Bellflower Extract, Geranium Extract, Red Vegetable Extract, Plantain Extract, Perilla Extract, and Sesame Extract, and Bark Extract, Garza Extract, Licorice Extract, Ginseng Extract, Red Ginseng Extract, Seokgok Extract, Coriander Extract, spectral extract, gut extract, gujeolcho extract, Selected from the group consisting of
  • the present invention also provides an antimicrobial composition consisting of the second complex extract obtained by treating the first complex extract with glycosidase.
  • the present invention provides an antimicrobial composition comprising the third complex extract, wherein the second complex extract is bioconverted by a specific lactic acid bacteria or a specific microorganism.
  • the present invention ⁇ , bean sprouts, green, shiitake mushrooms, red mushrooms, Ho Jang-geun, golden, yellow white, yellow yeon, yangyangyeop, broccoli, Sancho, three hundred seconds, eoseongcho, sari, Sesin, sound At least one selected from the group consisting of bamboo shoots, pine needles, knee roots, jinjin mugwort, buttercup, ginger, venom, yacon, schisandra chinensis, indongcho, grapefruit, Jangsaeng bellflower, ghost, red pepper, plantain, perilla, and wormwood From the group consisting of licorice, incense, ginseng, Seokgok, cilantro, spectators, guk, gujeolcho, KUJIGON, golden man, golden cherry tree, Gilkyung, mandang, sweet ginseng, sugar citron, lavender, rosemary, garlic, Ecklonia
  • the present invention ⁇ , bean sprouts, green onion, shiitake mushrooms, red mushrooms, Ho Jang-geun, golden, yellow white, yellow yeon, cedar leaf, broccoli, Sancho, triticale, seongseongcho, sari, Sesin, jinjak, pine needles, iron knee root, jinjinmu , At least one selected from the group consisting of buttercups, ginger, poison, yacon, schisandra, Indongcho, grapefruit, Jangsaeng bellflower, ghost, red vegetable, plantain, perilla, and mugwort, and bark skin, let's go, licorice, kangyang, ginseng, stone, Coriander, spectators, gukje, gujeolcho, kkujippong, geumyeongja, golden cherry tree, Gilkyung, mandang, sweet ginseng, sugar citrus, lavender, rosemary, garlic, Ecklonia, dandelion, white paper
  • the present invention comprises the steps of forming a mixture of the second complex extract with the lactic acid bacteria medium; Inoculating and fermenting a specific lactic acid bacterium or a specific microorganism in the mixture to bioconvert the second complex extract; And filtering the bioconverted second complex extract to obtain a third complex extract.
  • the present invention provides a composite natural preservative comprising the antimicrobial composition as an active ingredient.
  • the composite natural preservative according to the present invention may exist in various forms such as solid powder form, emulsion form, or liquid form, and is preferably in the form of emulsion in consideration of storage stability and ease of handling. Looking at the manufacturing method of the composite natural preservative in the form of an emulsion according to the present invention, for example, adding an oily medium and an emulsion stabilizer to the third complex extract and emulsifying and nano-dispersing by applying a shear force.
  • the antimicrobial composition or composite natural preservative according to the present invention may be added as a functional ingredient such as antiseptic to anti-oxidant in the manufacture of cosmetics, food, or food.
  • the antimicrobial composition according to the present invention is composed of extracts extracted from natural raw materials, processed extracts enzymatically processed, or processed extracts obtained by bioconverting the enzymatically processed processed extracts to specific lactic acid bacteria or specific microorganisms, thereby providing a broad antimicrobial spectrum, It has similar antimicrobial activity, and excellent antioxidant efficacy on synthetic preservatives. In addition, it is extracted from natural raw materials and is safe for human skin and has no acute oral toxicity.
  • complex natural preservatives which are decolorized and deodorized by enzymatic treatment and contain an antimicrobial composition consisting of processed extracts which are bioconverted by a specific lactic acid bacterium or a specific microorganism as an active ingredient, are in solid powder form, liquid form, or emulsion during manufacture. Easy to manufacture in form
  • the composite natural preservative according to the present invention is excellent in compatibility with other components and is very stable in formulation when preparing a cosmetic composition, a food composition, or a feed composition.
  • Figure 2 is a flat plate diffusion method using a paper disk to the antimicrobial activity of the composite natural preservatives (dilution concentration 0.01% (w / v), 0.05% (w / v)) prepared in Preparation Example 9 of the present invention 3 shows the antimicrobial activity of the composite natural preservative [dilution concentration 0.5 (w / v)] prepared in Preparation Example 9 of the present invention by a plate medium diffusion method using a paper disk. One result is shown.
  • Figure 4 shows the antioxidant efficacy of the composite natural preservative prepared in Preparation Example 9 of the present invention.
  • FIG. 5 is a graph showing the results of evaluation of the antiseptic effect by the Challenge Test of a care cream containing a complex natural preservative prepared in Preparation Example 9 of the present invention
  • Figure 6 is a composite prepared in Preparation Example 9 of the present invention A graph showing the antiseptic effect by the Challenge Test of a toner containing a natural preservative.
  • One aspect of the present invention relates to an antimicrobial composition consisting of extracts extracted from natural raw materials and a method for preparing the same.
  • the antimicrobial composition of the present invention comprises a first complex extract comprising an extract extracted from two or more natural raw materials.
  • the antimicrobial composition of the present invention preferably comprises a second complex extract obtained by treating the first complex extract with glycosidase, and more preferably the second complex extract is bioconverted by a specific lactic acid bacterium or a specific microorganism. It consists of a third complex extract.
  • the antimicrobial composition according to the present invention will be described by dividing into a first complex extract, a second complex extract and a third complex extract.
  • the antimicrobial composition of the present invention is an example, ⁇ extract, bean sprout extract, green onion extract, shiitake mushroom extract, mt. Mushroom extract, K. koji extract, golden extract, yellow baek extract, yellow yeon extract, cedar leaf extract, brokerage extract, sancho extract, triticale extract , Echochocho extract, Pear Extract, Secinin Extract, Root Extract, Pine Needle Extract, Knee Root Extract, Phosphorus Root Extract, Buttercup Extract, Ginger Extract, Poison Extract, Yacon Extract, Schisandra Extract, Honeysuckle Extract, Grapefruit Extract, Jangsaeng Bellflower Extract, Spirit At least one extract selected from the group consisting of extracts, red vegetable extracts, plantain extracts, perilla extracts, and wormwood extracts, bark extracts, bedrooms extracts, licorice extracts, nectarine extracts, red ginseng extracts, Seokok extracts, coriander extracts, spectral extracts, Guarium extract, gujeol
  • the antimicrobial composition of the present invention is composed of a first complex extract including a cedar leaf extract, three hundred vine extract, Eungchocho extract, and jinjin mugwort extract, preferably the first composite extract further comprises Ecklonia cava extract.
  • the antimicrobial activity of the antimicrobial composition consisting of the first complex extract is further increased.
  • the content in the complex extract of each extract extracted from each natural raw material is not particularly limited, generally 20 to 200 parts by weight, 300 to 200 parts by weight, 20 to 200 parts by weight of Echo extract, Injin mugwort extract 20 ⁇ 200 parts by weight and optionally 20-200 parts by weight of Ecklonia cava extract.
  • Description of the main raw material of the first complex extract according to the present invention is as follows.
  • Eoseongcho is named after its fishy fishy smell.
  • the three hundred perennial herbaceous perennial plant is a perennial herb of more than three hundred dicotyledonous plants. Three hundred seconds is the name given because the roots, leaves, and flowers are white.
  • Injin mugwort is a perennial plant of the dicotyledon plant Lantern Asteraceae, mainly distributed in Korea, Japan, China, etc., and often grows in sandy lands near streams.
  • Rhododendron It is an evergreen juniper tree of the dicotyledon azalea, Rhododendron, which is distributed mainly in Mt. Baekdu, Korea and eastern Siberia, and grows under the forest of high mountains.
  • Ecklonia cava is a perennial seaweed with brown seaweed kelp, seaweed, distributed mainly in the south coast of Korea and Japan, and grows deep in the lunch. Other raw materials are omitted from the description.
  • the composite extract according to the present invention includes various antibacterial active substances, antioxidant active substances and the like contained in the extract raw material as the active substance.
  • the active substances are mainly flavonoids, polyphenols or terpenes, specifically quercitrin, isoquercitrin, quercetin, rutin, myrcene, myrcene, Mircetin, Seanol, methyl-N-nonene, bacalin, bacicalein, and the like, but are not limited thereto.
  • the method for producing the first composite extract according to the present invention is ⁇ , bean sprouts, green onion, shiitake mushrooms, red mushrooms, Ho Jang Geun, golden, yellow white, yellow yeon, yang hyang leaf, broccoli, Sancho, three hundred seconds, Eoseongcho, sari, Sesin, sound At least one selected from the group consisting of bamboo shoots, pine needles, knee roots, jinjin mugwort, buttercup, ginger, venom, yacon, schisandra chinensis, indongcho, grapefruit, Jangsaeng bellflower, ghost, red pepper, plantain, perilla, and wormwood From the group consisting of licorice, incense, ginseng, Seokgok, cilantro, spectators, guk, gujeolcho, KUJIGON, golden man, golden cherry tree, Gilkyung, mandang, sweet ginseng, sugar citron, lavender, rosemary, garlic, Ecklonia cava,
  • the method for producing a first composite extract according to the present invention is an extract containing an antimicrobial active ingredient by a solvent extraction method, or supercritical extraction method from the extract raw material containing cedar leaf, three hundred vine, eoseongcho, jinjin worm, and optionally Ecklonia cava Obtaining a; And filtering the extract including the antimicrobial active ingredient into an extract and a residual extract raw solid; and concentrating the separated extract to obtain a first complex extract.
  • the solvent used may be water, alcohol having 1 to 5 carbon atoms, ethyl acetate, chloroform, acetone, 1,2-hexanediol, 1,3-butanediol, butylene glycol, water It is also possible to use a mixed solvent in which at least one selected from the remaining solvents is mixed. Of these, considering the hazards to the human body, it is preferable that the solvent is a mixed solvent of water, an alcohol having 1 to 5 carbon atoms or a mixture of water and an alcohol having 1 to 5 carbon atoms.
  • the extraction temperature of the solvent extraction method is not particularly limited, and is preferably characterized in that 40 ⁇ 80 °C.
  • the use ratio of the extraction raw material to the extraction solvent is not particularly limited, and typically, about 300 to 1000 parts by weight of the extraction solvent is used based on 100 parts by weight of the extraction raw material. Extraction time typically requires about 1 to 3 hours, but is not limited thereto.
  • the first complex extract according to the present invention may be preferably extracted by a supercritical extraction method.
  • the extraction yield is higher than that of the solvent extraction method, and the first composite extract extracted by the supercritical extraction method reduces odor and increases the transparency of the liquid form (or the size of the color) than that extracted by the solvent extraction method. Is reduced).
  • the supercritical extraction method uses a mixture of 1 to 20 parts by weight of an auxiliary solvent with respect to 100 parts by weight of carbon dioxide as a main solvent as a reaction medium, wherein diethylamine or triethylamine is methanol, ethanol, water, 1, It is composed of an alkaline cosolvent dissolved in 2 to 20% (vol%, v / v) in 2-hexanediol, 1,3-butanediol, butylene glycol, or a mixed solvent thereof.
  • extraction temperature is about 70 ⁇ 90 °C and extraction pressure is about 4000 ⁇ 6000 PSI.
  • Extracts extracted by solvent extraction or supercritical extraction are separated into extracts and residual extract raw solids by a filter cloth or filter paper having a pore size of micrometers, wherein the extract is concentrated to form a first complex extract in liquid form.
  • the concentrated first complex extract may be prepared as a first complex extract in powder form by drying.
  • the drying method is not particularly limited, but is preferably lyophilized in order to minimize thermal deformation of the active ingredient.
  • the residual extract raw material solid may be used to extract the active ingredient again by solvent extraction or supercritical extraction.
  • the antimicrobial composition of the present invention is another example, ⁇ extract, bean sprout extract, green onion extract, shiitake mushroom extract, mt. Mushroom extract, kojang-geun extract, golden extract, baekbaek extract, yeonyeon extract, cedar leaf extract, brokerage extract, sancho extract, triticale extract , Echochocho extract, Pear Extract, Secinin Extract, Root Extract, Pine Needle Extract, Knee Root Extract, Phosphorus Root Extract, Buttercup Extract, Ginger Extract, Poison Extract, Yacon Extract, Schisandra Extract, Honeysuckle Extract, Grapefruit Extract, Jangsaeng Bellflower Extract, Spirit At least one extract selected from the group consisting of extracts, red vegetable extracts, plantain extracts, perilla extracts, and wormwood extracts, bark extracts, bedrooms extracts, licorice extracts, nectarine extracts, red ginseng extracts, Seokok extracts, coriander extracts, spectral extracts, Gua
  • the antimicrobial composition of the present invention comprises a second complex extract obtained by enzymatic treatment of the first complex extract, including cedar leaf extract, triticale extract, Echocho extract, Injin mugwort extract, and optionally Ecklonia cava extract.
  • the first complex extract provided for the enzyme treatment may use a concentrated form in the manufacturing process or may use a solid powder form.
  • An enzyme used in enzyme treatment is glycosidase, and glycosidase is also called glycoside hydrolase, and has a function of hydrolyzing glycoside bonds of glycosides or small sugars.
  • glycosidase treatment in the present invention the extraction raw material itself can be decomposed to facilitate the extraction and increase the extraction yield, and to enable the extraction of the active ingredient of various forms to increase the antibacterial activity of the complex extract. have.
  • glycosidase treatment serves to deodorize and decolorize the extract by decomposing off-flavor or color components contained in the extract extracted by solvent extraction or supercritical extraction.
  • the removal of impurities by the membrane filter which mentions glycosidase treatment later is made smooth.
  • Method for producing a second complex extract according to the present invention ⁇ , bean sprouts, green onion, shiitake mushrooms, red mushrooms, Ho Jang Geun, golden, yellow white, yellow yeon, yangyangyeop, brokerage, Sancho, three hundred vine, Eoseongcho, sari, Sesin, sound At least one selected from the group consisting of bamboo shoots, pine needles, knee roots, jinjin mugwort, buttercup, ginger, venom, yacon, schisandra chinensis, indongcho, grapefruit, Jangsaeng bellflower, ghost, red pepper, plantain, perilla, and wormwood From the group consisting of licorice, incense, ginseng, Seokgok, cilantro, spectators, guk, gujeolcho, KUJIGON, golden man, golden cherry tree, Gilkyung, mandang, sweet ginseng, sugar citron, lavender, rosemary, garlic, Ecklonia cava,
  • the method for producing a second complex extract according to the present invention is an extract containing an antimicrobial active ingredient by a solvent extraction method, or a supercritical extraction method from the extract raw material including cedar leaf, three hundred vine, eoseongcho, Injin mugwort, and optionally Ecklonia cava Obtaining a; Enzymatic treatment by adding and reacting glycosidase to the extract comprising the antimicrobial active ingredient; Filtering the enzyme-treated extract into an extract and a residual extract raw solid; And concentrating the separated extract to obtain a second complex extract.
  • glucosidase used is not particularly limited, and specifically, amylae, amylae, cellulase, xylanase, galactose, galactose, lactase, maltase (maltase) or mixed enzymes thereof, and the like, and are preferably amylases.
  • Commercially available glucosidases include Viscozyme ® , Econase ® , Fungamyl ® , Sebamyl ® , and Clariceb ® .
  • reaction temperature, reaction pH, and reaction time of the first complex extract for glucosidase treatment vary depending on the enzyme used. Typically, the reaction temperature is 40-60 ° C., the reaction pH is 4-6, and the reaction time is 1-. 3 hours. In addition, the amount of enzyme is about 0.1 ⁇ 1 parts by weight based on 100 parts by weight of the extraction raw materials in consideration of economics such as enzyme processing time, enzyme cost.
  • Extracts treated with glucosidase are separated into extracts and residual extract solids by filtration, where filtration separates the extracts into extracts and residual extract solids by filter cloth or filter paper with a pore size of micrometers.
  • the primary filtered extract is filtered with a membrane filter having a pore size of nanometers to remove impurities (odor component or color component).
  • Glucosidase is difficult to perform due to the large load when filtration of the extract passed through the filter cloth or filter paper from the extract raw material by solvent extraction method or supercritical extraction method with a membrane filter having a pore size of nanometers. Enzymatically treated and primary filtered extract is easily filtered by the membrane filter, the odor or components that contribute to the color can be removed.
  • the filtrate obtained by filtration in the glycosidase treated extract is concentrated to form a second complex extract in liquid form.
  • the concentrated second complex extract may be prepared in powder form by drying.
  • the drying method is not particularly limited, but is preferably lyophilization in order to minimize the thermal deformation of the active ingredient.
  • the residual extract raw material solid may be used to extract the active ingredient again by solvent extraction or supercritical extraction.
  • the antimicrobial composition of the present invention is another example, ⁇ extract, bean sprout extract, green onion extract, shiitake mushroom extract, chopped mushroom extract, rhododendron extract, golden extract, baekbaek extract, yellow lotus extract, cedar leaf extract, brokerage extract, sancho extract, triticale Extract, Echochocho extract, Pear Extract, Secinin Extract, Root Extract, Pine Needle Extract, Knee Root Extract, Injin mugwort Extract, Buttercup Extract, Ginger Extract, Poison Extract, Yacon Extract, Schisandra Extract, Indongcho Extract, Grapefruit Extract, Jangsaeng Bellflower Extract, One or more extracts selected from the group consisting of geranium extract, red radish extract, plantain extract, perilla extract, and wormwood extract, and bark extract, bedrooms extract, licorice extract, nectarine extract, red ginseng extract, Seokok extract, coriander extract, spectral extract , Guru extract, gujeolcho extract, kujippong
  • the antimicrobial composition of the present invention is a lactic acid bacteria or specific lactic acid bacteria or a second complex extract obtained by treating the first complex extract including glycosidase, and optionally Ecklonia extract, Echinacea extract, and Ecklonia cava extract And a third complex extract bioconverted by the microorganism.
  • the lactic acid bacteria or the second complex extract provided for the specific microorganism fermentation may use a concentrated form in the manufacturing process or may use a solid powder form.
  • the specific lactic acid bacteria or specific microorganisms used to bioconvert the second complex extract are not particularly limited as long as they are harmless to the human body, and specifically, Lactobacillus and Streptococcus ), Pediococcus, Leuconostoc and the like.
  • the antimicrobial spectrum of the composite extract is much wider, and various active substances are produced to increase the fungal activity.
  • the compatibility with other ingredients is increased in the manufacture of a composite natural preservative to be described later to produce a cosmetic comprising a composite natural preservative as an active ingredient, thereby improving the stability of the formulation.
  • the method for producing a third complex extract forming a mixture of the second complex extract with lactic acid bacteria medium; Inoculating and fermenting a specific lactic acid bacterium or a specific microorganism in the mixture to bioconvert the second complex extract; And filtering the bioconverted second composite extract to obtain a third composite extract.
  • the filtration of the step of obtaining the third complex extract is a filter cloth or filter paper having a pore size in micrometers. Filtration by is sufficient.
  • the filtration of the step of obtaining the third complex extract is a filter cloth having a pore size of micrometer unit or It is preferred to consist of primary filtration with filter paper and secondary filtration with membrane filter with pore size in nanometers.
  • the third complex extract obtained by filtration can then be prepared as a third complex extract in concentrated liquid form, and the concentrated third complex extract can also be prepared in powder form by drying.
  • the drying method is not particularly limited, but is preferably lyophilized in order to minimize thermal deformation of the active ingredient.
  • the composite natural preservative is a mixture of two or more substances extracted from natural substances, and means a substance in which such a mixture acts in combination and exhibits a synergistic effect. Alternatively, it may be referred to as naturalotics.
  • the composite natural preservative according to the present invention may also be composed of the antimicrobial composition itself, and preferably further include various auxiliary additives so long as the antiseptic activity is not inhibited.
  • the composite natural preservative may be present in liquid form, solid powder form, or emulsion form, and is preferably in emulsion form in view of storage stability and ease of handling.
  • the method of preparing a composite natural preservative in the form of an emulsion includes, for example, adding an oily medium and an emulsion stabilizer to the third complex extract and emulsifying it by applying shear force.
  • auxiliary additives there are various types of auxiliary additives to be used depending on the purpose of the auxiliary additives, specifically glycerin (Clycerin), caster oil (Gaster), glycine (Glycine), Tween (Dextrin), Lactic acid (Lactic acid) ).
  • Another aspect of the invention relates to the use of said antimicrobial composition or composite natural preservative.
  • the antimicrobial composition according to the present invention is composed of extracts of various natural raw materials, and the antimicrobial composition has a broad spectrum antimicrobial range and the same antimicrobial activity as conventional synthetic preservatives, and further has a high antioxidant effect, safety on human skin, And acute oral administration, and non-toxic, and other effects have a whitening effect, wrinkle improvement, anti-inflammatory action, atopy improvement, acne healing, anti-aging derived from natural products. Therefore, the composite natural preservative including the antimicrobial composition or the antimicrobial composition according to the present invention as an active ingredient in various products such as pharmaceuticals, cosmetics, foods, or feeds for the purpose of preservation, improvement of immune function due to enhanced antioxidant activity, cell regeneration, etc.
  • the antimicrobial composition or the composite natural preservative according to the present invention can be used for the purpose of, and particularly preferably used in cosmetics or food.
  • its amount is preferably about 0.05 to 2.0% by weight based on the total weight of the product, and when used in food, the amount is based on the total weight of the product. 0.03-0.3 weight% is preferable.
  • cosmetics comprising the antimicrobial composition or the composite natural preservative of the present invention by selecting suitable formulations and additives as is well known.
  • suitable formulations and additives include various creams, lotions, skins, shampoos, rinses, cleansing agents, face washes, soaps, treatments, essences, and the like.
  • face wash creams face wash foams
  • cleansing creams cleansing milks, cleansing lotions
  • Cosmetics containing the antimicrobial composition or complex natural preservative of the present invention include aqueous vitamins, oily vitamins, polymer peptides, polymer polysaccharides, sphingolipids and the like.
  • the aqueous vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like.
  • Their salts thiamine hydrochloride, sodium ascorbate salt, etc.
  • derivatives ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.
  • the oil-based vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1- ⁇ -tocopherol, d- ⁇ -tocopherol, d- ⁇ -tocopherol) and the like.
  • Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic palmitate, dl- ⁇ -tocopherol acetate, dl- ⁇ -tocopherolvitamin E nicotinic acid, DL-pantothenyl alcohol, D-pantothenyl alcohol , Pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention.
  • the polymer peptide may be any compound as long as it can be incorporated into cosmetics.
  • collagen hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given.
  • the polymer polysaccharide may be any compound as long as it can be blended into cosmetics.
  • hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned.
  • chondroitin sulfate or its salt can be normally purified from a mammal or fish.
  • the sphingolipid may be any compound as long as it can be blended into cosmetics.
  • ceramide, pit spingosine, sphingolipid lipid and the like can be given.
  • the blending component that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavors, and the like.
  • Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.
  • the moisturizing agent examples include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.
  • emollient examples include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.
  • Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE-POP (polyoxyethylene-polyoxypropylene) copolymer, POE-POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc.
  • anionic surfactant fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like.
  • amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.
  • Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide
  • Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, carbon black, and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments
  • Metal soaps such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl- ⁇ -alanine calcium, N-lauroyl- ⁇ -alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-l
  • Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid.
  • paraaminobenzoic acid ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid.
  • Examples of the alcohol include higher alcohols such as cetyl alcohol.
  • the temperature of the extraction reactor was maintained at about 80 °C for 3 hours to extract the antimicrobial active ingredients of natural raw materials.
  • the extract was filtered with a filter cloth to separate the extract and the remaining extract raw solids, and the filtered extract was concentrated to a concentration of about 2.0 Brix using a reduced pressure evaporator to obtain 140 kg of the first complex extract in the form of a concentrate.
  • the first complex extract in the form of a concentrate was lyophilized to obtain a first complex extract in the form of a powder.
  • the temperature of the extraction reactor was maintained at about 80 °C for 3 hours to extract the antimicrobial active ingredients of natural raw materials.
  • the extract was filtered with a filter cloth to separate the extract and the remaining extract raw solid, and the filtered extract was concentrated to a concentration of about 2 Brix using a reduced pressure evaporator to obtain 140 kg of the first complex extract in the form of a concentrate.
  • the first complex extract in the form of a concentrate was lyophilized to obtain a first complex extract in the form of a powder.
  • the temperature of the extraction reactor was maintained at about 80 °C for 3 hours to extract the antimicrobial active ingredients of natural raw materials. After lowering the temperature of the extraction reactor to about 55 °C and adjusting the pH of the extract to about 5.5, the extract is charged with about 200 g of glycosidase (Fungamyl ® , Sebamyl ® , and Clariseb ® ; Special Enzyme, USA) for 3 hours After the reaction, the temperature of the extract was raised to about 80 ° C. to inactivate the enzyme.
  • glycosidase Fungamyl ® , Sebamyl ® , and Clariseb ® ; Special Enzyme, USA
  • the enzyme-treated extract was first filtered through a filter cloth to separate the extract and the remaining extract raw material solids, and then the filtered extract was passed through a membrane filter (pore size: 0.45 ⁇ m) and filtered secondly to remove impurities using a reduced pressure evaporator. Components that contribute to color, odor, etc.) were removed.
  • the extract passed through the membrane filter was concentrated to a concentration of about 2.5 Brix to give 140 kg of the second complex extract in the form of a concentrate.
  • the second complex extract in the form of a concentrate was lyophilized to obtain a second complex extract in the form of a powder.
  • the temperature of the extraction reactor was maintained at about 80 °C for 3 hours to extract the antimicrobial active ingredients of natural raw materials. After lowering the temperature of the extraction reactor to about 55 °C and adjusting the pH of the extract to about 5.5, the extract is charged with about 200 g of glycosidase (Fungamyl ® , Sebamyl ® , and Clariseb ® ; Special Enzyme, USA) for 3 hours After the reaction, the temperature of the extract was raised to about 80 ° C.
  • glycosidase Fungamyl ® , Sebamyl ® , and Clariseb ® ; Special Enzyme, USA
  • the enzyme-treated extract was first filtered through a filter cloth to separate the extract and the remaining extract raw material solids, and then the filtered extract was passed through a membrane filter (pore size: 0.45 ⁇ m) and filtered secondly to remove impurities using a reduced pressure evaporator. Components that contribute to color, odor, etc.) were removed.
  • the extract passed through the membrane filter was concentrated to a concentration of about 2.5 Brix to give 140 kg of the second complex extract in the form of a concentrate.
  • the second complex extract in the form of a concentrate was lyophilized to obtain a second complex extract in the form of a powder.
  • LB variable medium Lia-Bertani media
  • 94 L of the second complex extract in the form of a concentrate obtained in Preparation Example 3 were added thereto, and the fermenter was sterilized by steam.
  • the temperature of the sterilized fermenter was maintained at 40 ° C, and 5 l of lactic acid bacteria culture solution was added to the fermenter to inoculate.
  • the inoculated lactic acid bacteria medium was precultured with Lactobacillus bacteria in LB variable medium (Luria-Bertani media), and the cell count concentration of lactic acid bacteria was 1 ⁇ 10 7 cfu (Colony Forming Unit) / ml.
  • LB medium was used consisting of 10 g / L tryptone, 10 g / L sodium chloride (NaCl), 5 g / l yeast extract on the distilled water base.
  • the temperature of the fermenter inoculated with the lactic acid bacteria culture medium was adjusted to about 35 ° C. and fermented for about 12 hours to bioconvert the second composite extract. Thereafter, the fermentation broth of the fermenter was filtered sequentially using a filter cloth and a membrane filter to obtain 100 kg of the third complex extract in liquid form. In addition, it was concentrated and dried to obtain a third complex extract in the form of a powder.
  • the LB variable medium was composed of 10 g / l tryptone, 10 g / l sodium chloride (NaCl) and 5 g / l yeast extract on distilled water base.
  • the temperature of the fermenter inoculated with the lactic acid bacteria culture medium was adjusted to about 35 ° C. and fermented for about 12 hours to bioconvert the second composite extract. Thereafter, the fermentation broth of the fermenter was filtered sequentially using a filter cloth and a membrane filter to obtain 100 kg of the third complex extract in liquid form. In addition, it was concentrated and dried to obtain a third complex extract in the form of a powder.
  • the color and smell of the solvent extract of the natural raw material, the enzyme-treated extract treated with the enzyme extract and the enzyme-treated extract, and the extract obtained by bioconversion of the enzyme-treated extract by lactic acid bacteria fermentation were observed.
  • the composite extract in powder form prepared by Preparation Example 2, Preparation Example 4, and Preparation Example 6 was dissolved in distilled water to a concentration of 2.5% (w / v), and color and smell were observed.
  • Preparation Example 2 had a deep navy blue
  • Preparation Example 4 had a dark yellow color
  • Preparation Example 6 had a light yellow color.
  • Antimicrobial activity against the harmful strains of the complex extracts was determined by plate medium diffusion using a paper disk.
  • the strain was incubated in Muller Hinton broth (Difco, USA) medium for 12 hours, and the cell count concentration was adjusted to 1.5 ⁇ 10 4 cfu (Colony Forming Unit) / ml, followed by Muller Hinton Agar (Difco, USA) was spread evenly over the medium.
  • Agar discs with a diameter of 6 mm and 1.5 mm (Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) were attached to the agar selection medium on which the harmful strains were smeared.
  • the composite extract in powder form prepared in Preparation Examples 1 to 6 was dissolved in distilled water to a concentration of 0.5% (w / v), 40 ⁇ l of which was absorbed into a paper disk, and then optimal culture of the harmful strains. Incubate at temperature for 24 hours. The antimicrobial effect was confirmed by measuring the size of the harmful strain growth inhibitory rings produced after the culture, and the results are shown in Table 1.
  • SA Salmonella (Staphylococcus aureus ATCC29213)
  • BS Bacillus subtilis ATCC21770
  • EC E. coli (Escherichia coli ATCC25922)
  • PA Pseudomonas aeruginosa ATCC27853
  • AF is Atopic bacillus (Aspergillus f75um).
  • AN stands for yeast mold (Aspergillus niger ATCC96918)
  • PF stands for blue mold (Penicillium funiculosum ATCC11797)
  • CA stands for Candida albicans ATCC10231.
  • High performance liquid chromatography (HPLC) analysis was performed to observe the antimicrobial active substances of the second complex extract and the third complex extract in powder form obtained in Preparation Examples 4 and 6.
  • HPLC high performance liquid chromatography
  • acetonitrile and 0.5% of phosphoric acid were mixed at 27:73 (v / v).
  • the column was a u-Bondapak C18 silica column. It was used to dissolve the extract in the form of distilled water.
  • 1 shows the results of high performance liquid chromatography (HPLC) analysis of the second complex extract (top) and the third complex extract (bottom). As shown in Figure 1 it can be seen that the rich antibacterial active material is produced by the lactic acid bacteria fermentation.
  • the third complex extract in liquid form obtained in preparation 6 was concentrated to about 2.5 Brix. 18 kg of the third complex extract concentrated in the emulsification tank. 60 kg of glycerin and 22 kg of castor oil were added thereto, and the temperature of the emulsifying tank was increased to about 70 to 75 ° C., and homogenized by stirring with a homogenizer (about 220 to 250 rpm). . Thereafter, the temperature of the emulsion tank was lowered to about 32 to 35 ° C., and the pH of the contents homogenized with lactic acid was adjusted to about 3 to 5 to prepare a composite natural preservative in the form of an emulsion.
  • Antimicrobial activity against the harmful strains of the composite natural preservative prepared in Preparation Example 9 was measured by the plate medium diffusion method using a paper disk (paper disk).
  • the strain was incubated in Muller Hinton broth (Difco, USA) medium for 12 hours, and the cell count concentration was adjusted to 1.5 ⁇ 10 4 cfu (Colony Forming Unit) / ml, followed by Muller Hinton Agar (Difco, USA) was spread evenly over the medium.
  • Agar discs with a diameter of 6 mm and 1.5 mm (Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) were attached to the agar selection medium on which the harmful strains were smeared.
  • the composite natural preservative in the form of emulsion prepared in Preparation Example 9 was dissolved in distilled water to a concentration of 0.01% (w / v), 0.05% (w / v) and 0.5% (w / v), 40 ⁇ l of which was taken. After absorption on paper disks, the cultures were incubated for 24 hours at the optimum culture temperature of the harmful strains. The antimicrobial effect was confirmed by measuring the size of the growth inhibitory strain produced after the culture, and the results are shown in Table 2. As a control, only the distilled water was absorbed into the paper disk.
  • MIT / CMIT active ingredient: MIT (2-methyl-4-isothiazolin-3-one) / CMIT (5-chloro-2-methyl] -4-isothiazolin-3-one mixture] methyl paraben, Hydantol [active ingredient: 5,5-di (phenyl) imidazolidine-2,4-dione], and DF-100 (grape seed extract, Extract of grapefruit seed) was used.
  • SA Salmonella (Staphylococcus aureus ATCC29213)
  • BS Bacillus subtilis ATCC21770
  • EC E. coli (Escherichia coli ATCC25922)
  • PA Pseudomonas aeruginosa ATCC27853
  • AF is Atopy bacterium (Aspergillus f75um).
  • AN stands for yeast mold (Aspergillus niger ATCC96918)
  • PF stands for blue mold (Penicillium funiculosum ATCC11797)
  • CA stands for Candida albicans ATCC10231.
  • NP3 composite natural preservative according to the present invention showed almost the same antimicrobial activity as MIT / CMIT or Hydantol, which is a conventional synthetic preservative. It can be seen that the spectrum is also very wide.
  • Figure 2 is a flat plate diffusion method using a paper disk to the antimicrobial activity of the composite natural preservatives (dilution concentration 0.01% (w / v), 0.05% (w / v)) prepared in Preparation Example 9 of the present invention 3 shows the antimicrobial activity of the composite natural preservative [dilution concentration 0.5 (w / v)] prepared in Preparation Example 9 of the present invention by a plate medium diffusion method using a paper disk. One result is shown.
  • Hazardous strains cultured for 24 hours were placed in 3 ml of medium (brain heart infusion broth), and at the same time, the complex natural preservatives prepared in Preparation Example 9 were added to respective concentrations, and then cultured at 37 ° C. for 24 hours. Dilution of the composite natural preservative prepared in Preparation Example 9 was used a 2-fold serial dilution method.
  • the MIC of the complex natural preservative prepared in Preparation Example 9 for the harmful strain was 0.016 to 0.312% (w / v), showed the lowest antibacterial activity against Bacillus subtilis ATCC21770, Candida It showed the highest antimicrobial activity against (Candida albicans ATCC32354).
  • DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to confirm the antioxidant effect of the composite natural preservative prepared in Preparation Example 9.
  • DPPH is a colored radical that can be used to directly determine the radical removal capacity of a sample.
  • a sample (complex natural preservative prepared in Preparation Example 9 and a comparative sample) was dissolved in distilled water or a solvent to prepare for each concentration. After mixing 1 ml of 100 uM DPPH, the mixture was reacted at room temperature for 30 minutes. Absorbance was measured at 517 nm to determine the amount of DPPH remaining. The relative antioxidant effect of each sample was expressed as a percentage based on the control group (control: test group consisting of DPPH, distilled water or solvent only, without a sample).
  • Figure 4 shows the antioxidant efficacy of the composite natural preservative prepared in Preparation Example 9 of the present invention.
  • the composite natural preservative prepared in Preparation Example 9 of the present invention was higher in antioxidant activity than polyphenols, BHA (butylated hydroxyanisole), and BHT (butylated hydroxytoluene) of green tea powder. From these antioxidant activity results, it can be seen that the composite natural preservative prepared in Preparation Example 9 of the present invention can be used as a feed additive.
  • LD 50 half lethal dose
  • BW body weight
  • the mean score is 0.00-0.75, it is non-irritating, 0.76-1.50 is non-irritating, 1.51-2.50 is light stimulation, 2.51-4.00 is medium stimulation, and 4.01 or more is determined as strong stimulation.
  • Care cream containing a composite natural preservative prepared in Preparation Example 9 was prepared in the composition of Table 5.
  • Toner including the composite natural preservative prepared in Preparation Example 9 was prepared in the composition shown in Table 6.
  • the preservative effect of the composite natural preservative prepared in Preparation Example 9 was evaluated using the care creams prepared in Preparation Examples 10 to Comparative Preparation Examples 1 and 2 and the toners prepared in Preparation Example 11 and Comparative Preparation Examples 3 and 4, respectively.
  • the method of evaluating antiseptic effects was based on the Microbial Challenge Test proposed by the European Pharmacopoeia Commission (EP) in 1996 for topical preparations (Letters in Applied Microbiology 2002, 35, 385-389).
  • the harmful strains used were Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger.
  • the preservative effect was measured up to 7 days.
  • the initial cell count concentration of the harmful strain was adjusted to 1 ⁇ 10 8 cfu (Colony Forming Unit) / mL (cosmetic sample), and the number of strains that survived in the sample was incubated at 30 to 32 ° C. while the mixed sample was cultured. It was measured according to the water test method. The antiseptic effect was expressed by the change in the average cell concentration of the five harmful strains used.
  • FIG. 5 is a graph showing the results of evaluation of the antiseptic effect by the Challenge Test of a care cream containing a complex natural preservative prepared in Preparation Example 9 of the present invention
  • Figure 6 is a composite prepared in Preparation Example 9 of the present invention A graph showing the antiseptic effect by the Challenge Test of a toner containing a natural preservative.
  • the composite natural preservative prepared in Preparation Example 9 of the present invention showed an antiseptic effect similar to that of the conventional synthetic preservatives methylparaben and propyl paraben for cosmetics.
  • Antimicrobial composition according to the present invention is composed of extracts of various natural raw materials, the antimicrobial composition has a broad spectrum antimicrobial range and the same antimicrobial activity as conventional synthetic preservatives, has a high antioxidant effect and safety for human skin In addition, it is nontoxic in acute oral administration. Therefore, the antimicrobial composition according to the present invention may be used as an active ingredient of a composite natural preservative, and may be applied to cosmetics, food, and the like, and may be applied to feed additives to improve the immune function of an animal.

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Abstract

La présente invention concerne une composition antimicrobienne comprenant un premier extrait composite contenant un ou plusieurs extraits choisis dans le groupe constitué d'extraits d'arrow-root, de germes de soja, de laitue de mer, de champignons shiitake, de champignons Sarcodon aspratus, de renouée, de scutellaire, d'écorce d'arbre à liège de l'Amour, de coptide, de feuilles de Ledumpalustre var. diversipilosum, de brocoli, de bois de poivre, de houstonie, de houttuynia cordata, de lespédéza, d'asaret, de Rumex crispus L., d'aiguilles de pin, de racines d'achyranthes, d'artemisia capillaris, de vallisnérie américaine, de gingembre, d'araliae continentalis radix, de poire de terre, de schizandre, de Lonicera japonica, de pamplemousse, de racines de fleurs de Jang Saeng ballon, de Grifola umbellata, de chou rouge, de bananier plantain, de périlla et d'Artemisia lavandulaefolia, ainsi que dans un autre groupe constitué d'extraits de Terminalia chebula, de Dalbergia odorifera, de racine de sophora, de Coriandri Fructus, de rhizome de Crassirhizomae, de Dianthus chinensis, de chrysanthème de Sibérie, de Cudrania tricuspidata, de meliae fructus, de Rhus laevigata, de Platycodon glandiflorum, de champignons shimegi, de miltiorrhiza Bunge, de Citrus grandis Osbek, de lavande, de romarin, d'ail, de Lindera glauca, de pissenlit, d'Angelicae Dahuricae Radix, d'agaric blanc et de Sclerotium Poriae Cocos. La composition antimicrobienne de la présente invention peut comprendre un second extrait composite obtenu par traitement du premier extrait composite au moyen de glycosidase, ou peut comprendre un troisième extrait composite bioconverti à partir du second extrait composite à l'aide de microorganismes spécifiques. Comme la composition antimicrobienne selon la présente invention comprend des extraits d'ingrédients naturels, des extraits traités obtenus par traitement des extraits au moyen d'enzymes ou d'autres extraits traités obtenus par bioconversion des extraits traités au moyen de bactéries lactiques ou de microorganismes spécifiques, la composition présente un large spectre antimicrobien, une action antimicrobienne similaire à celle des antiseptiques de synthèse types et un remarquable effet antioxydant. En outre, la composition de la présente invention peut être appliquée sans risque sur la peau humaine et ne présente pas de toxicité orale aiguë, car ladite composition est extraite d'ingrédients naturels. De plus, un antiseptique naturel composite contenant, en tant que principe actif, la composition antimicrobienne comprenant des extraits traités, qui sont dépigmentés et désodorisés par un traitement enzymatique, et qui est bioconvertie par des bactéries lactiques ou des microorganismes spécifiques, peut être facilement fabriquée sous la forme d'une poudre solide, d'un liquide ou d'une émulsion. En outre, l'antiseptique composite naturel selon la présente invention présente une remarquable compatibilité avec d'autres ingrédients et, en conséquence, les formes dosifiées fabriquées pour être intégrées dans des compositions cosmétiques, alimentaires ou dans des aliments pour animaux peuvent se révéler très stables.
PCT/KR2010/000920 2010-02-12 2010-02-12 Composition antimicrobienne contenant des extraits d'ingrédients naturels, antiseptique naturel composite et procédé de fabrication associé WO2011099665A1 (fr)

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CN109395136A (zh) * 2018-08-31 2019-03-01 阿坝州慈愿民族传统文化有限责任公司 一种降香型藏香及其制备方法
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WO2021256865A1 (fr) * 2020-06-17 2021-12-23 다당앤바이오 주식회사 Composition pharmaceutique contenant une fraction de saururus chinensis, et procédé de préparation de celle-ci
CN113925065A (zh) * 2021-11-25 2022-01-14 上海健康医学院 一种纳米有机无机复合抗菌剂及其制备方法
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DE102011088000A1 (de) 2011-12-08 2012-08-23 Henkel Ag & Co. Kgaa Verwendung von Platycodinen zur Beeinflussung des natürlichen Pigmentierungsprozesses
DE102011087980A1 (de) 2011-12-08 2012-09-06 Henkel Kgaa Deodorants und Antitranspirants mit Haar aufhellender Wirkung
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US10583178B2 (en) 2012-07-05 2020-03-10 Nutramax Laboratories, Inc. Compositions comprising sulforaphane or a sulforaphane precursor and a mushroom extract or powder
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KR101863299B1 (ko) 2016-04-01 2018-06-01 (주)에스디생명공학 미르센을 유효성분으로 함유하는 피부 주름 예방 또는 개선용 조성물
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KR101951649B1 (ko) * 2018-05-28 2019-02-25 주식회사 제이투케이바이오 레스베라트롤 함량이 증가된 호장근추출물의 제조방법과 이를 함유하는 항진균용 조성물
CN109395136A (zh) * 2018-08-31 2019-03-01 阿坝州慈愿民族传统文化有限责任公司 一种降香型藏香及其制备方法
CN109329705B (zh) * 2018-10-31 2021-10-01 海南职业技术学院 一种微生物食品抗氧化剂的制作方法
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CN109601814A (zh) * 2018-12-10 2019-04-12 武汉轻工大学 一种天然食品防腐剂及其制备方法和应用
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WO2020128991A1 (fr) * 2018-12-21 2020-06-25 Covertis Substance biologiquement active, son procede de fabrication et son utilisation comme agent protecteur d'un tissu biologique
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CN115715196A (zh) * 2020-06-17 2023-02-24 巴玛科生物株式会社 包含三白草馏分的药物组合物及其制备方法
WO2021256865A1 (fr) * 2020-06-17 2021-12-23 다당앤바이오 주식회사 Composition pharmaceutique contenant une fraction de saururus chinensis, et procédé de préparation de celle-ci
CN115715196B (zh) * 2020-06-17 2024-05-28 巴玛科生物株式会社 包含三白草馏分的药物组合物及其制备方法
CN112353739A (zh) * 2020-11-23 2021-02-12 上海健康医学院 一种中草药牙膏及其生产方法
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CN114848553A (zh) * 2022-05-20 2022-08-05 山东福瑞达生物股份有限公司 一种天然防腐剂及其应用
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