WO2011069992A2 - Muteins of human lipocalin 2 (lcn2, hngal) with affinity for a given target - Google Patents

Muteins of human lipocalin 2 (lcn2, hngal) with affinity for a given target Download PDF

Info

Publication number
WO2011069992A2
WO2011069992A2 PCT/EP2010/069028 EP2010069028W WO2011069992A2 WO 2011069992 A2 WO2011069992 A2 WO 2011069992A2 EP 2010069028 W EP2010069028 W EP 2010069028W WO 2011069992 A2 WO2011069992 A2 WO 2011069992A2
Authority
WO
WIPO (PCT)
Prior art keywords
mutein
sequence
lcn2
protein
muteins
Prior art date
Application number
PCT/EP2010/069028
Other languages
English (en)
French (fr)
Other versions
WO2011069992A3 (en
Inventor
Arne Skerra
Michaela Gebauer
Dominik Hinz
Sabine Rauth
Gabriele Matschiner
Original Assignee
Pieris Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN201080055370.5A priority Critical patent/CN102770764B/zh
Priority to JP2012542505A priority patent/JP5913120B2/ja
Priority to EP10784824.4A priority patent/EP2510357B1/de
Priority to KR1020187007781A priority patent/KR101974044B1/ko
Priority to EP15186647.2A priority patent/EP2990798B1/de
Priority to US13/514,133 priority patent/US9549968B2/en
Priority to DK10784824.4T priority patent/DK2510357T3/en
Priority to KR1020127017730A priority patent/KR101842076B1/ko
Application filed by Pieris Ag filed Critical Pieris Ag
Priority to EP19198835.1A priority patent/EP3660510A3/de
Priority to RU2012128586/10A priority patent/RU2564125C2/ru
Priority to BR112012013662-3A priority patent/BR112012013662B1/pt
Priority to CA2779562A priority patent/CA2779562C/en
Priority to AU2010329995A priority patent/AU2010329995B2/en
Publication of WO2011069992A2 publication Critical patent/WO2011069992A2/en
Publication of WO2011069992A3 publication Critical patent/WO2011069992A3/en
Priority to US15/380,168 priority patent/US10618941B2/en
Priority to US16/812,069 priority patent/US11827681B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00041—Use of virus, viral particle or viral elements as a vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Epidemiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
PCT/EP2010/069028 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (lcn2, hngal) with affinity for a given target WO2011069992A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
EP19198835.1A EP3660510A3 (de) 2009-12-07 2010-12-07 Muteine von humanem lipocalin 2 (lcn2, hngal) mit affinität für ein bestimmtes zielmolekül
JP2012542505A JP5913120B2 (ja) 2009-12-07 2010-12-07 所定の標的に対して親和性を有するヒトリポカリン２（Ｌｃｎ２、ｈＮＧＡＬ）の突然変異タンパク質
RU2012128586/10A RU2564125C2 (ru) 2009-12-07 2010-12-07 Мутеины липокалина 2 человека (lcn2, hngal) с аффинностью для определенной мишени
EP15186647.2A EP2990798B1 (de) 2009-12-07 2010-12-07 Muteine von humanem lipocalin 2 (lcn2, hngal) mit affinität für ein bestimmtes zielmolekül
US13/514,133 US9549968B2 (en) 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (LcnC,hNGAL) with affinity for a given target
DK10784824.4T DK2510357T3 (en) 2009-12-07 2010-12-07 MUTEINS OF HUMAN LIPOCALIN 2 (LCN2, HNGAL) WITH AFFINITY FOR A SPECIFIC TARGET
KR1020127017730A KR101842076B1 (ko) 2009-12-07 2010-12-07 주어진 표적에 대한 친화성을 갖는 인간의 리포칼린 2의 뮤테인
CN201080055370.5A CN102770764B (zh) 2009-12-07 2010-12-07 对给定靶标具有亲和力的人脂质运载蛋白2（Lcn2，hNGAL）的突变蛋白
EP10784824.4A EP2510357B1 (de) 2009-12-07 2010-12-07 Muteine von humanem lipocalin 2 (lcn2, hngal) mit affinität für ein bestimmtes zielmolekül
KR1020187007781A KR101974044B1 (ko) 2009-12-07 2010-12-07 주어진 표적에 대한 친화성을 갖는 인간의 리포칼린 2의 뮤테인
BR112012013662-3A BR112012013662B1 (pt) 2009-12-07 2010-12-07 Muteínas de lipocalina associada à gelatinase de neutrófilo humano (lcn2, hngal), seu uso e seus métodos de geração e produção, molécula de ácido nucleico, célula hospedeira, composição farmacêutica e kit de diagnóstico ou analítico
CA2779562A CA2779562C (en) 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (lcn2, hngal) with affinity for a given target
AU2010329995A AU2010329995B2 (en) 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (Lcn2, hNGAL) with affinity for a given target
US15/380,168 US10618941B2 (en) 2009-12-07 2016-12-15 Muteins of human lipocalin 2 (Lcn2,hNGAL) with affinity for a given target
US16/812,069 US11827681B2 (en) 2009-12-07 2020-03-06 Muteins of human lipocalin 2 (Lcn2, hNGAL) with affinity for a given target

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26709809P 2009-12-07 2009-12-07
US61/267,098 2009-12-07

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/514,133 A-371-Of-International US9549968B2 (en) 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (LcnC,hNGAL) with affinity for a given target
US15/380,168 Continuation US10618941B2 (en) 2009-12-07 2016-12-15 Muteins of human lipocalin 2 (Lcn2,hNGAL) with affinity for a given target

Publications (2)

Publication Number Publication Date
WO2011069992A2 true WO2011069992A2 (en) 2011-06-16
WO2011069992A3 WO2011069992A3 (en) 2011-08-11

Family

ID=43597993

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/069028 WO2011069992A2 (en) 2009-12-07 2010-12-07 Muteins of human lipocalin 2 (lcn2, hngal) with affinity for a given target

Country Status (12)

Country Link
US (3) US9549968B2 (de)
EP (3) EP3660510A3 (de)
JP (4) JP5913120B2 (de)
KR (2) KR101842076B1 (de)
CN (1) CN102770764B (de)
AU (1) AU2010329995B2 (de)
BR (1) BR112012013662B1 (de)
CA (1) CA2779562C (de)
DK (2) DK2990798T3 (de)
RU (2) RU2564125C2 (de)
SG (1) SG10201408073XA (de)
WO (1) WO2011069992A2 (de)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013174783A1 (en) * 2012-05-23 2013-11-28 Pieris Ag Lipocalin muteins with binding-affinity for glypican-3 (gpc-3) and use of lipocalin muteins for target-specific delivery to cells expressing gpc-3
WO2016142314A1 (en) 2015-03-06 2016-09-15 Deutsches Krebsforschungszentrum FUSION PROTEINS COMPRISING A BINDING PROTEIN AND AN INTERLEUKIN-15 POLYPEPTIDE HAVING A REDUCED AFFINITY FOR IL15Ra AND THERAPEUTIC USES THEREOF
CN107403074A (zh) * 2017-06-09 2017-11-28 天津市湖滨盘古基因科学发展有限公司 一种突变蛋白的检测方法及装置
WO2018015380A1 (en) 2016-07-18 2018-01-25 Philippe Ulsemer Natural microorganisms which are naturally capable of binding toxins and/or toxin receptors
US10273275B2 (en) 2015-05-18 2019-04-30 Pieris Pharmaceuticals Gmbh Muteins of human lipocalin 2 with affinity for glypican-3 (GPC3) and methods of use thereof
AU2016212087B2 (en) * 2015-01-28 2019-11-07 Pieris Pharmaceuticals Gmbh Novel proteins specific for angiogenesis
WO2020201038A1 (en) 2019-03-29 2020-10-08 Pieris Pharmaceuticals Gmbh Inhaled administration of lipocalin muteins
US10913778B2 (en) 2015-05-18 2021-02-09 Pieris Pharmaceuticals Gmbh Anti-cancer fusion polypeptides, encoding nucleic acids and methods of using polypeptides
US10947284B2 (en) 2015-07-07 2021-03-16 Pieris Pharmaceuticals Gmbh Fusion molecules

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3660510A3 (de) * 2009-12-07 2020-07-08 Pieris Pharmaceuticals GmbH Muteine von humanem lipocalin 2 (lcn2, hngal) mit affinität für ein bestimmtes zielmolekül
WO2012022742A1 (en) * 2010-08-16 2012-02-23 Pieris Ag Binding proteins for hepcidin
US9758554B2 (en) 2012-01-31 2017-09-12 Technische Universitaet Muenchen Muteins of α1m lipocalin and method of production therefor
EP3441400B1 (de) 2012-11-19 2022-07-20 Pieris Pharmaceuticals GmbH Neuartige polypeptide mit spezifischer bindung und verwendungen davon
BR112016027024A2 (pt) 2014-05-22 2017-10-31 Pieris Pharmaceuticals Gmbh polipeptídeos de ligação específica e seus usos
AU2016258977C1 (en) 2015-05-04 2022-07-14 Pieris Pharmaceuticals Gmbh Anti-cancer fusion polypeptide
US11261221B2 (en) 2015-05-04 2022-03-01 Pieris Pharmaceuticals Gmbh Proteins specific for CD137
BR112018010887A2 (pt) * 2015-11-30 2018-11-21 Pieris Australia Pty Ltd polipeptídeo de fusão
TW201725212A (zh) * 2015-12-10 2017-07-16 第一三共股份有限公司 特異性於降鈣素基因相關胜肽的新穎蛋白
GB201615254D0 (en) 2016-09-08 2016-11-23 Ge Healthcare Bio Sciences Ab Novel Ligand and use thereof
EP3715370A1 (de) * 2019-03-28 2020-09-30 Technische Universität München Hochaffine anticaline gegen menschliches cd98hc

Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0330451A2 (de) 1988-02-22 1989-08-30 Toa Nenryo Kogyo Kabushiki Kaisha Für menschliches Normal-Serumalbumin kodierende cDNA und Verfahren zur Herstellung des Albumins
EP0361991A2 (de) 1988-08-05 1990-04-04 Rhone-Poulenc Sante Verfahren zur mikrobiologischen Herstellung von menschlichem Serumalbumin und anderen heterologen Proteinen in Hefe
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
WO1999016873A1 (de) 1997-09-26 1999-04-08 Arne Skerra Anticaline
WO1999064016A1 (en) 1998-06-08 1999-12-16 F. Hoffmann-La Roche Ag Use of peg-ifn-alpha and ribavirin for the treatment of chronic hepatitis c
WO2000075308A1 (de) 1999-06-08 2000-12-14 Pieris Proteolab Ag Muteine des bilin-bindungsproteins
US6177074B1 (en) 1995-11-02 2001-01-23 Schering Corporation Polyethylene glycol modified interferon therapy
US6403564B1 (en) 1998-10-16 2002-06-11 Schering Corporation Ribavirin-interferon alfa combination therapy for eradicating detectable HCV-RNA in patients having chronic hepatitis C infection
US6500930B2 (en) 1998-03-31 2002-12-31 Hemosol Inc. Hemoglobin-polysaccharide conjugates
WO2003029471A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of apolipoprotein d
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
US20030069395A1 (en) 2001-03-09 2003-04-10 Sato Aaron K. Serum albumin binding moieties
US6620413B1 (en) 1995-12-27 2003-09-16 Genentech, Inc. OB protein-polymer chimeras
US6696245B2 (en) 1997-10-20 2004-02-24 Domantis Limited Methods for selecting functional polypeptides
WO2005019256A2 (en) 2003-08-25 2005-03-03 Pieris Proteolab Ag Muteins of tear lipocalin
WO2005019254A1 (en) 2003-08-25 2005-03-03 Pieris Proteolab Ag Muteins of a bilin-binding protein with affinity for a given target
WO2006056464A2 (en) 2004-11-26 2006-06-01 Pieris Ag Compound with affinity for the cytotoxic t lymphocyte-associated antigen (ctla-4)
WO2007038619A2 (en) 2005-09-27 2007-04-05 Amunix, Inc. Proteinaceous pharmaceuticals and uses thereof

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
EP0257956B2 (de) 1986-08-19 2000-11-22 Genentech, Inc. Verwendung von Polypeptidwuchsstoffen und Zytokinen zur Herstellung eines Geräts und einer Dispersion
AU696387B2 (en) 1994-05-18 1998-09-10 Inhale Therapeutic Systems, Inc. Methods and compositions for the dry powder formulation of interferons
DE4417598A1 (de) 1994-05-19 1995-12-14 Max Planck Gesellschaft Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen
AU5132096A (en) 1995-01-30 1996-08-21 Terrapin Technologies, Inc. Glubodies - multiplicities of proteins capable of binding a variety of small molecules
DE19641876B4 (de) 1996-10-10 2011-09-29 Iba Gmbh Streptavidinmuteine
WO1998016873A1 (fr) 1996-10-14 1998-04-23 Firm Forsat Ltd. Procede de preparation de dispersions a base de composants chromogenes
US6020163A (en) 1997-08-06 2000-02-01 Zymogenetics, Inc. Lipocalin homolog
US20140080177A1 (en) 1997-09-26 2014-03-20 Pieris Ag Anticalins
CA2343917A1 (en) 1998-10-19 2000-04-27 Ariad Gene Therapeutics, Inc. Materials and methods involving conditional retention domains
ES2290023T3 (es) * 1999-03-04 2008-02-16 Praecis Pharmaceuticals Incorporated Moduladores de la agregacion del peptido beta-amiloide que comprenden d-aminoacidos.
JP2007284351A (ja) * 2004-07-27 2007-11-01 Osaka Bioscience Institute アミロイド蛋白質の凝集を抑制する物質とその作用
CA2659413C (en) * 2006-08-01 2016-06-14 Pieris Ag Muteins of tear lipocalin and methods for obtaining the same
CN101516907B (zh) * 2006-08-01 2015-08-26 皮里斯股份公司 泪液脂质运载蛋白的突变蛋白及其获得方法
JP2011501673A (ja) * 2007-10-19 2011-01-13 アボット・ラボラトリーズ グリコシル化された哺乳動物ｎｇａｌ及びその使用
AU2009264214B2 (en) 2008-06-24 2013-06-06 Technische Universitaet Muenchen Muteins of hNGAL and related proteins with affinity for a given target
EP3660510A3 (de) * 2009-12-07 2020-07-08 Pieris Pharmaceuticals GmbH Muteine von humanem lipocalin 2 (lcn2, hngal) mit affinität für ein bestimmtes zielmolekül
WO2012022742A1 (en) * 2010-08-16 2012-02-23 Pieris Ag Binding proteins for hepcidin
JP6100694B2 (ja) 2010-11-15 2017-03-22 ピエリス ファーマシューティカルズ ゲーエムベーハー グリピカン−３（ｇｐｃ３）に対して親和性を有するヒトリポカリン２の突然変異タンパク質
EP3441400B1 (de) * 2012-11-19 2022-07-20 Pieris Pharmaceuticals GmbH Neuartige polypeptide mit spezifischer bindung und verwendungen davon
US10526382B2 (en) * 2015-01-28 2020-01-07 Pieris Pharmaceuticals Gmbh Human neutrophil gelatinase-associated lipocalin (hNGAL) muteins capable of binding angiopoietin-2 (Ang-2) and methods of use thereof
AU2016221816B2 (en) 2015-02-18 2020-07-02 Pieris Pharmaceuticals Gmbh Novel proteins specific for pyoverdine and pyochelin
US11261221B2 (en) 2015-05-04 2022-03-01 Pieris Pharmaceuticals Gmbh Proteins specific for CD137
CN107787327B (zh) 2015-05-18 2022-02-08 皮里斯制药有限公司 对磷脂酰肌醇聚糖-3（gpc3）具有亲和力的人脂质运载蛋白2的突变蛋白
TW201725212A (zh) 2015-12-10 2017-07-16 第一三共股份有限公司 特異性於降鈣素基因相關胜肽的新穎蛋白

Patent Citations (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0330451A2 (de) 1988-02-22 1989-08-30 Toa Nenryo Kogyo Kabushiki Kaisha Für menschliches Normal-Serumalbumin kodierende cDNA und Verfahren zur Herstellung des Albumins
EP0361991A2 (de) 1988-08-05 1990-04-04 Rhone-Poulenc Sante Verfahren zur mikrobiologischen Herstellung von menschlichem Serumalbumin und anderen heterologen Proteinen in Hefe
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
US6177074B1 (en) 1995-11-02 2001-01-23 Schering Corporation Polyethylene glycol modified interferon therapy
US6620413B1 (en) 1995-12-27 2003-09-16 Genentech, Inc. OB protein-polymer chimeras
WO1999016873A1 (de) 1997-09-26 1999-04-08 Arne Skerra Anticaline
US6696245B2 (en) 1997-10-20 2004-02-24 Domantis Limited Methods for selecting functional polypeptides
US6500930B2 (en) 1998-03-31 2002-12-31 Hemosol Inc. Hemoglobin-polysaccharide conjugates
WO1999064016A1 (en) 1998-06-08 1999-12-16 F. Hoffmann-La Roche Ag Use of peg-ifn-alpha and ribavirin for the treatment of chronic hepatitis c
US6403564B1 (en) 1998-10-16 2002-06-11 Schering Corporation Ribavirin-interferon alfa combination therapy for eradicating detectable HCV-RNA in patients having chronic hepatitis C infection
WO2000075308A1 (de) 1999-06-08 2000-12-14 Pieris Proteolab Ag Muteine des bilin-bindungsproteins
US20030069395A1 (en) 2001-03-09 2003-04-10 Sato Aaron K. Serum albumin binding moieties
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
WO2003029463A2 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
WO2003029471A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of apolipoprotein d
WO2005019256A2 (en) 2003-08-25 2005-03-03 Pieris Proteolab Ag Muteins of tear lipocalin
WO2005019254A1 (en) 2003-08-25 2005-03-03 Pieris Proteolab Ag Muteins of a bilin-binding protein with affinity for a given target
WO2005019255A1 (en) 2003-08-25 2005-03-03 Pieris Proteolab Ag Muteins of tear lipocalin
WO2006056464A2 (en) 2004-11-26 2006-06-01 Pieris Ag Compound with affinity for the cytotoxic t lymphocyte-associated antigen (ctla-4)
WO2007038619A2 (en) 2005-09-27 2007-04-05 Amunix, Inc. Proteinaceous pharmaceuticals and uses thereof

Non-Patent Citations (78)

* Cited by examiner, † Cited by third party
Title
ALTSCHUL, S. F. ET AL., NUCL. ACIDS RES., vol. 25, 1997, pages 3389 - 3402
ALTUVIA ET AL., J. MOL. BIOL., vol. 249, 1995, pages 244 - 250
AMSTUTZ, P. ET AL., CURR. OPIN. BIOTECHNOL., vol. 12, 2001, pages 400 - 405
BACHMANN, MICROBIOL. REV., vol. 54, 1990, pages 130 - 197
BESTE ET AL., PNAS, vol. 96, 1999, pages 1898 - 903
BESTE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 1898 - 1903
BITTKER ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 1024 - 1029
BOS ET AL., BUSINESS BRIEFING: PHARMATECH, 2003, pages 1 - 6
BREUSTEDT ET AL., BIOCHIM. BIOPHYS. ACTA, vol. 1764, 2006, pages 161 - 173
BRODERS ET AL.: "Hyperphage. Improving antibody presentation in phage display", METHODS MOL. BIOL., vol. 205, 2003, pages 295 - 302
BRODY; HOLTZMANN, ANNU. REV. NEUROSCI., vol. 31, 2008, pages 175 - 193
BRUCKDORFER ET AL., CURR. PHARM. BIOTECHNOL., vol. 5, 2004, pages 29 - 43
BULLOCK ET AL., BIOTECHNIQUES, vol. 5, 1987, pages 376 - 378
CARNEMOLLA ET AL., INT. J. CANCER, vol. 68, 1996, pages 397 - 405
COLOWICK: "Solid-Phase Peptide Synthesis", 1997, ACADEMIC PRESS
DENNIS ET AL., J. BIOL. CHEM., vol. 277, 2002, pages 35035 - 35043
DODEL ET AL., LANCET NEUROLOGY, vol. 2, 2003, pages 215 - 220
EBBINGHAUS ET AL., CURR. PHARM. DES., vol. 10, 2004, pages 1537 - 1549
FLING; GREGERSON, ANAL. BIOCHEM., vol. 155, 1986, pages 83 - 88
FRANK, J. IMMUNOL. METHODS, vol. 262, 2002, pages 13 - 26
FUERTGES ET AL.: "The Clinical Efficacy of Poly(Ethylene Glycol)-Modified", PROTEINS J. CONTROL. RELEASE, vol. 11, 1990, pages 139 - 148
GAILLARD PJ ET AL., EXPERT OPIN DRUG DELIV., vol. 2, no. 2, 2005, pages 299 - 309
GAILLARD PJ ET AL., INTERNATIONAL CONGRESS SERIES, vol. 1277, 2005, pages 185 - 198
GASTEIGER ET AL., NUCLEIC ACIDS RES., vol. 31, 2003, pages 3784 - 3788
GASTEIGER ET AL.: "ExPASy: the proteomics server for in-depth protein knowledge and analysis", NUCLEIC ACIDS RES., vol. 31, 2003, pages 3784 - 3788
GENNARO; GENNARO: "Remington: The Science and Practice of Pharmacy", 2000, LIPPINCOTT WILLIAMS & WILKINS
GOETZ ET AL., BIOCHEMISTRY, vol. 39, 2000, pages 1935 - 1941
GRONWALL ET AL., J. BIOTECHNOL., vol. 128, 2007, pages 162 - 183
HAASS; SELKOE, NAT. REV. MOL. CELL. BIOL., vol. 8, 2007, pages 101 - 112
HORTSCHANSKY ET AL., PROTEIN SCI., vol. 14, 2005, pages 1753 - 1759
HOYER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 105, 2008, pages 5099 - 5104
KARLSSON ET AL., J. IMMUNOL. METHODS, vol. 145, 1991, pages 229 - 240
KASPAR ET AL., INT. J. CANCER, vol. 118, 2006, pages 1331 - 1339
KAY, B.K. ET AL.: "Phage Display of Peptides and Proteins - A Laboratory Manual", 1996, ACADEMIC PRESS
KHURANA ET AL., J. STRUCT. BIOL., vol. 151, 2005, pages 229 - 238
KIM ET AL., J. AM. CHEM. SOC., vol. 131, 2009, pages 3565 - 3576
KONIG, T.; SKERRA, A., J. IMMUNOL. METHODS, vol. 218, 1998, pages 73 - 83
LEAHY ET AL., PROTEINS, vol. 19, 1994, pages 48 - 54
LICHTLEN; MOHAJERI, J. NEUROCHEM., vol. 104, 2007, pages 859 - 874
LLOYD-WILLIAMS ET AL.: "Chemical Approaches to the Synthesis of Peptides and Proteins", 1997, CRC PRESS
LOWMAN, H.B., ANNU. REV. BIOPHYS. BIOMOL. STRUCT., vol. 26, 1997, pages 401 - 424
MATEO ET AL., HYBRIDOMA, vol. 19, no. 6, 2000, pages 463 - 471
MEIDAN; MICHNIAK, AM. J. THER., vol. 11, no. 4, 2004, pages 312 - 316
MORETTO ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 11436 - 11445
MURAKAMI ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 76 - 81
OSBORN ET AL., J. PHARMACOL. EXP. THER., vol. 303, 2002, pages 540 - 548
OSBORN, B.L. ET AL., J. PHARMACOL. EXP. THER., vol. 303, 2002, pages 540 - 548
PINI ET AL., J. BIOL. CHEM., vol. 273, 1998, pages 21769 - 21776
PINI, A. ET AL., COMB. CHEM. HIGH THROUGHPUT SCREEN., vol. 5, 2002, pages 503 - 510
PUJUGUET ET AL., AM. J. PATHOL., vol. 148, pages 579 - 592
REDL, B., BIOCHIM. BIOPHYS. ACTA, vol. 1482, 2000, pages 241 - 248
RODI, D.J.; MAKOWSKI, L., CURR. OPIN. BIOTECHNOL., vol. 10, 1999, pages 87 - 93
SAMBROOK ET AL.: "Molecular Cloning. A Laboratory Manual", 1989, COLD SPRING HARBOR PRESS
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SCHLEHUBER ET AL., J MOL BIOL, vol. 297, 2000, pages 1109 - 20
SCHLEHUBER ET AL., J. MOL. BIOL., vol. 297, 2000, pages 1105 - 1120
SCHLEHUBER, S.; SKERRA, A.: "Duocalins, engineered ligand-binding proteins with dual specificity derived from the lipocalin fold", BIOL. CHEM., vol. 382, 2001, pages 1335 - 1342
SCHLIEMANN; NERI, BIOCHIM. BIOPHYS. ACTA, vol. 1776, 2007, pages 175 - 192
SCHMIDT, T.G.M. ET AL., J. MOL. BIOL., vol. 255, 1996, pages 753 - 766
SCHMIDT; SKERRA, NAT. PROTOC., vol. 2, 2007, pages 1528 - 1535
SCHONFELD ET AL., PROC. NATL. ACAD. SCI. USA, vol. 106, 2009, pages 8198 - 8203
SKERRA, GENE, vol. 151, 1994, pages 131 - 135
SKERRA, J. BIOTECHNOL., vol. 74, 2001, pages 257 - 275
STUDIER; MOFFAT, J. MOL. BIOL., vol. 189, 1986, pages 113 - 130
TARTOF; HOBBS, BETHESDA RESEARCH LABORATORY FOCUS, vol. 9, 1987, pages 12
VAJO; DUCKWORTH, PHARMACOL. REV., vol. 52, 2000, pages 1 - 9
VENTURI ET AL., J. MOL. BIOL., vol. 315, 2002, pages 1 - 8
VIRNEKÄS B; GE L; PLIICKTHUN A; SCHNEIDER KC; WELLNHOFER G; MORONEY SE: "Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis", NUCLEIC ACIDS RES, vol. 22, 1994, pages 5600 - 5607
VOGT; SKERRA, CHEMBIOCHEM, vol. 5, 2004, pages 191 - 199
VOGT; SKERRA, J. MOL. RECOGNIT., vol. 14, no. 1, 2001, pages 79 - 86
VOSS; SKERRA, PROTEIN ENG., vol. 10, 1997, pages 975 - 982
WANG, L. ET AL., SCIENCE, vol. 292, 2001, pages 498 - 500
WANG, L.; SCHULTZ, P.G., CHEM. COMM., vol. 1, 2002, pages 1 - 11
WILSON, D.S. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 3750 - 3755
YANISCH-PERRON ET AL., GENE, vol. 33, 1985, pages 103 - 119
ZACCOLO ET AL., J. MOL. BIOL., vol. 255, 1996, pages 589 - 603
ZARDI ET AL., EMBO J, vol. 6, 1987, pages 2337 - 2342
ZARDI ET AL., EMBO JOURNAL, vol. 6, 1987, pages 2337 - 2342

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013174783A1 (en) * 2012-05-23 2013-11-28 Pieris Ag Lipocalin muteins with binding-affinity for glypican-3 (gpc-3) and use of lipocalin muteins for target-specific delivery to cells expressing gpc-3
US9522940B2 (en) 2012-05-23 2016-12-20 Pieris Pharmaceuticals Gmbh Lipocalin muteins with binding-affinity for glypican-3 (GPC-3) and use of lipocalin muteins for target-specific delivery to cells expressing GPC-3
AU2016212087B2 (en) * 2015-01-28 2019-11-07 Pieris Pharmaceuticals Gmbh Novel proteins specific for angiogenesis
WO2016142314A1 (en) 2015-03-06 2016-09-15 Deutsches Krebsforschungszentrum FUSION PROTEINS COMPRISING A BINDING PROTEIN AND AN INTERLEUKIN-15 POLYPEPTIDE HAVING A REDUCED AFFINITY FOR IL15Ra AND THERAPEUTIC USES THEREOF
US10273275B2 (en) 2015-05-18 2019-04-30 Pieris Pharmaceuticals Gmbh Muteins of human lipocalin 2 with affinity for glypican-3 (GPC3) and methods of use thereof
US10787491B2 (en) 2015-05-18 2020-09-29 Pieris Pharmaceuticals Gmbh Nucleic acid molecules encoding muteins of human lipocalin 2 with affinity for glypican-3 (GPC3)
US10913778B2 (en) 2015-05-18 2021-02-09 Pieris Pharmaceuticals Gmbh Anti-cancer fusion polypeptides, encoding nucleic acids and methods of using polypeptides
US10947284B2 (en) 2015-07-07 2021-03-16 Pieris Pharmaceuticals Gmbh Fusion molecules
WO2018015380A1 (en) 2016-07-18 2018-01-25 Philippe Ulsemer Natural microorganisms which are naturally capable of binding toxins and/or toxin receptors
CN107403074A (zh) * 2017-06-09 2017-11-28 天津市湖滨盘古基因科学发展有限公司 一种突变蛋白的检测方法及装置
WO2020201038A1 (en) 2019-03-29 2020-10-08 Pieris Pharmaceuticals Gmbh Inhaled administration of lipocalin muteins

Also Published As

Publication number Publication date
KR20180031081A (ko) 2018-03-27
US9549968B2 (en) 2017-01-24
JP2022160410A (ja) 2022-10-19
AU2010329995B2 (en) 2015-09-17
DK2990798T3 (da) 2019-12-02
RU2012128586A (ru) 2014-01-20
JP2018148914A (ja) 2018-09-27
US20130079286A1 (en) 2013-03-28
CA2779562A1 (en) 2011-06-16
JP2016105709A (ja) 2016-06-16
AU2010329995A1 (en) 2012-05-10
BR112012013662B1 (pt) 2022-08-02
RU2707126C2 (ru) 2019-11-22
US10618941B2 (en) 2020-04-14
KR101974044B1 (ko) 2019-04-30
EP2510357A2 (de) 2012-10-17
WO2011069992A3 (en) 2011-08-11
CA2779562C (en) 2019-12-31
EP2990798A1 (de) 2016-03-02
EP3660510A3 (de) 2020-07-08
DK2510357T3 (en) 2016-07-18
US11827681B2 (en) 2023-11-28
US20170114109A1 (en) 2017-04-27
EP2510357B1 (de) 2016-03-30
RU2015137158A (ru) 2018-12-25
CN102770764B (zh) 2016-04-20
KR101842076B1 (ko) 2018-03-27
SG10201408073XA (en) 2015-01-29
JP2013512683A (ja) 2013-04-18
CN102770764A (zh) 2012-11-07
RU2564125C2 (ru) 2015-09-27
EP3660510A2 (de) 2020-06-03
EP2990798B1 (de) 2019-09-25
KR20120117815A (ko) 2012-10-24
JP6346159B2 (ja) 2018-06-20
JP2021006024A (ja) 2021-01-21
US20200308237A1 (en) 2020-10-01
RU2015137158A3 (de) 2018-12-25
BR112012013662A2 (pt) 2020-11-03
JP5913120B2 (ja) 2016-05-11

Similar Documents

Publication Publication Date Title
US11827681B2 (en) Muteins of human lipocalin 2 (Lcn2, hNGAL) with affinity for a given target
US10046027B2 (en) Muteins of tear lipocalin and methods for obtaining the same
US9751920B2 (en) Muteins with tear lipocalin having affinity to human c-met receptor tyrosine kinase and methods for obtaining the same
JP2018519802A (ja) グリピカン−３（ｇｐｃ３）に対する親和性を有するヒトリポカリン２のムテイン

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201080055370.5

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10784824

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2010329995

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2779562

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2010329995

Country of ref document: AU

Date of ref document: 20101207

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2012542505

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2010784824

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 5964/DELNP/2012

Country of ref document: IN

ENP Entry into the national phase

Ref document number: 20127017730

Country of ref document: KR

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2012128586

Country of ref document: RU

WWE Wipo information: entry into national phase

Ref document number: 13514133

Country of ref document: US

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112012013662

Country of ref document: BR

REG Reference to national code

Ref country code: BR

Ref legal event code: B01E

Ref document number: 112012013662

Country of ref document: BR

Free format text: APRESENTAR DOCUMENTO DE CESSAO DA PRIORIDADE US 61/267,098 ASSINADO E DATADO POR GABRIELE MATSCHINER CONTENDO, PELO MENOS, NUMERO E DATA DE DEPOSITO DO DOCUMENTO DE PATENTE QUE ESTA SENDO CEDIDO, UMA VEZ QUE O DOCUMENTO ENVIADO NA PETICAO NO 020120072023 NAO APRESENTA OS DADOS IDENTIFICADORES DA PRIORIDADE, CONTEMPLANDO SOMENTE O TITULO NAO POSSIBILITANDO DETERMINAR A QUAL PRIORIDADE ELE SE REFERE.

ENP Entry into the national phase

Ref document number: 112012013662

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20120606