WO2011049334A2 - Gene oslrp pour augmenter la resistance au maladies de vegetaux et leur utilisation - Google Patents

Gene oslrp pour augmenter la resistance au maladies de vegetaux et leur utilisation Download PDF

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WO2011049334A2
WO2011049334A2 PCT/KR2010/007117 KR2010007117W WO2011049334A2 WO 2011049334 A2 WO2011049334 A2 WO 2011049334A2 KR 2010007117 W KR2010007117 W KR 2010007117W WO 2011049334 A2 WO2011049334 A2 WO 2011049334A2
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oslrp
plant
rice
gene
resistance
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WO2011049334A9 (fr
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황덕주
장지영
박은미
허성기
노태환
안일평
박상렬
김민갑
배신철
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대한민국(농촌진흥청장)
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/126—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for virus resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the present invention relates to the OsLRP gene and its use to enhance resistance to plant diseases, more specifically from rice OsLRP ( Oryza sativa leucine rich repeat protein)
  • OsLRP Oryza sativa leucine rich repeat protein
  • the present invention relates to a composition for enhancing plant disease resistance comprising a gene and a method of improving plant productivity by transforming plant cells with the recombinant vector.
  • Plants and crops such as rice, corn, and barley, are important food crops that provide food for humans, and these crops include black atrophy disease (black string organ disease), ogal disease, and streaked leaf blight and bacteria caused by viruses. There is a great deal of economic damage due to rice leaf blight caused by mildew blight and mold.
  • US Patent No. 6653533 discloses methods and materials for protecting plants from pathogens, but differs from the genes of the present invention.
  • the present invention has been made in accordance with the above requirements, the present invention introduces a recombinant vector containing OsLRP , a leucine rich repeat protein (LRP) gene derived from rice ( Oryza sativa ), rice blight, rice blast, rice streaks
  • OsLRP leucine rich repeat protein
  • LRP leucine rich repeat protein
  • the present invention is derived from rice OsLRP ( Oryza sativa leucine rich repeat protein) Provided are methods for enhancing plant disease resistance by transforming plant cells with a recombinant vector comprising a gene.
  • OsLRP Oryza sativa leucine rich repeat protein
  • the present invention also provides a plant and its seed having improved resistance to plant diseases transformed with the recombinant vector.
  • the present invention also provides a composition for enhancing plant disease resistance comprising the rice-derived OsLRP gene.
  • the present invention also provides a method of improving plant productivity by transforming plant cells with the recombinant vector.
  • the present invention can effectively enhance the resistance of plants to rice blight blight, rice blast, rice streaked blight, cabbage blight, etc. by transferring the OsLRP gene to a plant transformation expression vector.
  • X stands for XbaI
  • N stands for NotI
  • E stands for EcoRI.
  • Figure 2 is an electrophoresis picture showing the PCR results of the BAR primer of the rice transformant.
  • S represents the control transformed with the vector only, H represents the non-transgenic control, and the remaining number represents the transformant.
  • Figure 3 is a photograph and a graph showing the resistance test against rice leaf blight of OsLRP transformants.
  • NT is a non-transgenic control
  • P represents a control transformed with the vector only.
  • Figure 4 is a photograph showing the resistance assay against rice blast of OsLRP transformants. 18 and 19 represent OsLRP transformants.
  • Figure 6 is the result confirming the resistance effect of streaked leaf blight resistance of OsLRP transformants.
  • Figure 7 shows the results of detecting rice streak virus in the pathogen of OsLRP transformants and control.
  • NT Nontransformer, V; OsLRP transformation vector
  • Figure 9 shows the results confirming the effect of increasing the resistance to Chinese cabbage bruises of OsLRP transformants.
  • NT is a non-transgenic control and # 27 and # 40 represent OsLRP transformants.
  • the present invention is derived from rice OsLRP ( Oryza sativa leucine rich repeat protein) Plant cells are transformed with a recombinant vector containing the gene OsLRP Provided are methods for enhancing plant disease resistance comprising overexpressing a gene.
  • OsLRP Oryza sativa leucine rich repeat protein
  • the OsLRP gene may be preferably composed of a nucleotide sequence represented by SEQ ID NO: 1.
  • variants of the above nucleotide sequences are included within the scope of the present invention.
  • a variant is a nucleotide sequence that changes in base sequence but has similar functional properties to that of SEQ ID NO: 1.
  • the OsLRP gene comprises a nucleotide sequence having at least 70%, more preferably at least 80%, even more preferably at least 90%, most preferably at least 95% homology with the nucleotide sequence of SEQ ID NO: 1 can do.
  • the "% sequence homology" for a polynucleotide is identified by comparing two optimally arranged sequences with a comparison region, wherein part of the polynucleotide sequence in the comparison region is the reference sequence (addition or deletion) for the optimal alignment of the two sequences. It may include the addition or deletion (ie, gap) compared to).
  • the recombinant vector comprising the OsLRP gene of the present invention is preferably a plant expression vector, the plant expression vector including a promoter and terminator, the promoter may be a CaMV 35S, actin, ubiquitin, pEMU, MAS or histone promoter, but It is not limited.
  • the term “promoter” refers to a region of DNA upstream from a structural gene and refers to a DNA molecule to which an RNA polymerase binds to initiate transcription.
  • a "plant promoter” is a promoter capable of initiating transcription in plant cells.
  • a “constitutive promoter” is a promoter that is active under most environmental conditions and developmental conditions or cell differentiation. Constitutive promoters may be preferred in the present invention because selection of the transformants may be made by various tissues at various stages. Thus, the constitutive promoter does not limit the choice.
  • Terminator may be used a conventional terminator, and examples thereof include fine loam furnace sold synthase (NOS), rice ⁇ - amylase RAmy1 A terminator, De Pace (phaseoline) terminator, Agrobacterium Tome Pacific Enschede (Agrobacterium tumefaciens) ( Octopine) terminators, etc., but is not limited thereto. With regard to the need for terminators, it is generally known that such regions increase the certainty and efficiency of transcription in plant cells. The use of terminators is therefore highly desirable in the context of the present invention.
  • the plant disease may be one or more selected from the group consisting of rice leaf blight, rice blast, rice stripe leaf blight and cabbage blight, but is not limited thereto.
  • the present invention also provides a plant having improved resistance to plant diseases transformed with a recombinant vector comprising a rice-derived OsLRP gene.
  • a recombinant vector comprising a rice-derived OsLRP gene.
  • the OsLRP gene and recombinant vector are as described above.
  • the plant is Arabidopsis, potato, eggplant, tobacco, pepper, tomato, burdock, garland chrysanthemum, lettuce, bellflower, spinach, chard, sweet potato, celery, carrot, buttercup, parsley, cabbage, cabbage, gatchi, watermelon, melon, cucumber, Dicotyledonous plants such as pumpkins, gourds, strawberries, soybeans, green beans, kidney beans, peas, or monocotyledonous plants such as rice, barley, wheat, rye, corn, sugar cane, oats, and onions, preferably cabbage or rice. .
  • the present invention also provides seed of the plant.
  • said seed is a seed of cabbage or rice.
  • Plant transformation refers to any method of transferring DNA to a plant. Such transformation methods do not necessarily have a period of regeneration and / or tissue culture. Transformation of plant species is now common for plant species, including both dicotyledonous plants as well as monocotyledonous plants. In principle, any transformation method can be used to introduce hybrid DNA according to the invention into suitable progenitor cells. Methods include calcium / polyethylene glycol methods for protoplasts (Krens, FA et al., 1982, Nature 296, 72-74), electroporation of protoplasts (Shillito RD et al., 1985 Bio / Technol. 3, 1099-1102 ), Microscopic injection into plant elements (Crossway A. et al., 1986, Mol. Gen. Genet.
  • Preferred methods according to the invention include Agrobacterium mediated DNA delivery. Especially preferred is the use of the so-called binary vector technology as described in EP A 120 516 and US Pat. No. 4,940,838.
  • the "plant cells” used for plant transformation may be any plant cells.
  • the plant cells may be cultured cells, cultured tissues, cultured organs or whole plants, preferably cultured cells, cultured tissues or cultured organs and more preferably any form of cultured cells.
  • Plant tissue refers to the tissues of differentiated or undifferentiated plants, such as, but not limited to, roots, stems, leaves, pollen, seeds, cancer tissues and various types of cells used in culture, ie single cells, protoplasts. (protoplast), shoots and callus tissue. Plant tissue may be in planta or in organ culture, tissue culture or cell culture.
  • the present invention also provides a composition for enhancing plant disease resistance comprising the rice-derived OsLRP gene.
  • the OsLRP gene may be preferably composed of a nucleotide sequence represented by SEQ ID NO: 1.
  • the composition for enhancing plant disease resistance of the present invention includes an OsLRP gene derived from rice as an active ingredient, and the plant is transformed into the plant OsLRP by plants such as rice white leaf blight, rice blast, rice streaked blight, cabbage blight, etc. It can increase resistance to disease.
  • the present invention also provides a method for improving the productivity of a plant, comprising the step of overexpressing the OsLRP gene by transforming the plant cell with a recombinant vector comprising an OsLRP gene derived from rice.
  • Expression of the OsLRP gene in the plant of the present invention makes it resistant to plant diseases such as rice leaf blight, rice blast, rice streaked blight, cabbage blight, etc., plants transformed with the OsLRP gene are more productive than plants. This can be improved.
  • Plant transgenic vectors were made to overexpress the OsLRP gene in rice. First, amplify by 5L primer (5'-CGATATCGTGCGCGTCCAGAT-3 ': SEQ ID NO: 2) and 3' primer (5'-GCCTAGGCTAGCAGTTGGTGTC-3 ': SEQ ID NO: 3) using OsLRP cDNA clone and amplify by TOPO cloning vector. Was cloned in the T7 direction and the vector was named DJ129.
  • DJ129 was cut with EcoRV and SpeI to make an insert
  • pKS-LS9 was cut with EcoRV and XbaI to clone OsLRP to make DJ136
  • DJ136 was cut with XhoI and NotI to make OsLRP insert
  • pSBGMAR to XhoI and NotI Cleavage was cloned to make a vector for transforming OsLRP, DJ140, and a map of the vector is shown in FIG. 1.
  • OsLRP transfection vector was introduced into Agrobacterium by triparental mating and transformed into callus derived from hwacheong rice using the bacteria. Peeled Nakdong rice seeds were disinfected twice in 1 minute in 70% ethanol and 15 minutes in 50% Clorox solution and washed 3 times or more in 10 minutes with sterile water. The sterilized seeds were seeded in 2N6 medium (Hiei, Y. et al. 1994, The Plant Journal 6: 271-282) and incubated for 4 weeks at 28 ° C. in the dark to form embryogenic callus. Transfer to 2N6 medium and further incubated for 4 days.
  • LBA4404 strains carrying each gene for transformation were AB agar medium (KH 2 PO 4 3g / L, NaH) containing 50 mg / l of spectinomycin, 10 mg / l of tetracycline 2 PO 4 1g / l, NH 4 Cl 1g / l, MgSO 4 300mg / l, KCl 150mg / l, CaCl 2 10mg / l, FeSO 4 ⁇ 7H 2 O 5mg / l, Glucose 5g / l, Bacto Agar 15g / L) and inoculated to spread evenly over 2 days and incubated at 28 ° C.
  • KH 2 PO 4 3g / L, NaH containing 50 mg / l of spectinomycin, 10 mg / l of tetracycline 2 PO 4 1g / l, NH 4 Cl 1g / l, MgSO 4 300mg / l,
  • the callus was washed several times with sterile water containing 250 mg / l of cefotaxime to add 250 mg / l of Cytaxim and 6 mg / l of L-phosphinothricin (PPT). Transfer to 2N6-CP medium was incubated for 3 weeks in 28 °C, dark. After three weeks, the live callus without browning was transferred to a new 2N6-CP and incubated for two more weeks.
  • PPT L-phosphinothricin
  • the surviving callus after 2 weeks was 30 g / l of sucrose, 2 mg / l of kinineine, 0.5 mg / l of naphthaleneacetic acid (NAA), 250 mg / l of Cytaxime and phosphinothricin It was transferred to MSR-CP (pH 5.8) containing 3 mg / L and incubated for one month under constant light at 26 ° C. One month later, transfer to new MSR-CP, followed by further incubation, formed shoots were transferred to MS0 (pH 5.8) containing 30 g / L sucrose to form roots. After 7-8 days, when the roots formed well, they were purified and transplanted into the soil before being transferred to the greenhouse. When the plants adapted to some extent, the herbicide (0.1% bastard) was sprayed to check the herbicide resistance that does not turn brown.
  • Genomic DNA was isolated from the transgenic rice showing resistance to 0.1% bastard of Example 2 and the presence or absence of BAR gene was determined by the specific 5 'primer (5'-ACAGCGACCACGCTGTTGAA-3': SEQ ID NO: 4) and 3 of the BAR gene.
  • the polymerase chain reaction was carried out using 'primer (5'-TGCACCATCGTCAACCACTA-3': SEQ ID NO: 5) and the results are shown in FIG. 2.
  • Example 4 Resistance test against leaf blight of transgenic rice with OsLRP gene
  • the rice leaf blight is grown in PSA medium for 2 days, suspended in 1mM magnesium chloride, adjusted from OD 600 to 0.5, and the ends of the rice leaves are cut with scissors. It was inoculated by the method. The extent of pathogen progression was measured at 17 days after inoculation and the results are shown in FIG. 3. No. 22 and 26 showed a slight difference compared to the two control groups (NT, P), but the remaining transgenic rice showed little or no progress in the leaf blight, indicating that it was resistant to the rice leaf blight.
  • Example 5 Resistance test for blast disease of transgenic rice with OsLRP gene
  • the seed of the rice seed was stripped, sterilized with 50% Lax, healed in culture bottles, and grown for about a week, then spores derived from rice blast bacteria After spray inoculation examined the number of lesions. The results are shown in FIG. Compared to the non-transformed rice, which is a control, the transformed rice showed little occurrence of blast disease, thereby confirming that it was resistant to rice blast.
  • OsLRP transformed rice grown in greenhouses were harvested, and total RNA was isolated using Trizol (Invitrogen Co.). Reverse transcription was performed using oligo dT primers to identify the disease resistance induction marker gene OsPR10a (D38170) specific 5 'primer (5'-GCTACAGGCATCAGTGGTCA-3': SEQ ID NO: 6) and 3 'primer (5'-GACTCAAACGCCACGAGAGAAT- 3 ′: SEQ ID NO: 7) and also OsActin PCR (polymerase chain reaction) was performed 30 times at 55 °C annealing (annealing) temperature to confirm the expression of the OsPR10a gene is shown in Figure 5 the results. As shown in Figure 5, OsPR10a was confirmed that the induced expression compared to the control.
  • OsPR10a was confirmed that the induced expression compared to the control.
  • Rices infected with rice streak virus in greenhouses have high rates of supplementation of larvae.
  • the capsid protein specific 5 'primer (5'-AGCCACTCTAGCTGATTTG-3': SEQ ID NO: 8) and 3 'primer (5') of rice striated virus RNA3 -TGGTTATTTGGAAGGAGTG-3 ': SEQ ID NO: 9) and OsActin PCR (polymerase chain reaction) was performed 30 times at 55 ° C. annealing temperature to confirm the expression of the RSV-CP gene, and the results are shown in FIG. 7. . As can be seen in FIG. 7, a large amount of virus was detected in the control, but no virus was detected in the OsLRP transformant.
  • the OsLRP transformation vector prepared in Example 2 was transformed into Agrobacterium and transformed into stems derived from Seoul cabbage seed (Nongwoo Bio) using the bacterium. After 6 days of cancer culture and 1 day photoculture in MS medium, the stem was cut to 1 cm and CO medium (sucrose 30g / L, MS 4.43g / L, NAA 0.01g / L, BAP 0.05g / L, AgNO 3 0.08 g / l, pH5.8) and inoculated with the bacteria after 3 days photoculture. The bacteria were grown in 75 mg / L spectinomycin in LB, incubated at 37 ° C for 1 day, and prepared to be OD 0.8-1.0.
  • Cancer culture for 3 days after inoculation and washig solution (sucrose 30g / l, MS 4.43g / l, NAA 0.01g / l, BAP 0.05g / l, AgNO 3 0.08g / l, cefotaxime 250 mg / l, pH5.8) After washing with SM medium (sucrose 30g / l, MS 4.43g / l, NAA 0.01g / l, BAP 0.05g / l, AgNO3 0.08g / l, cefotaxime 250mg / l, PPT 3mg / l, pH5. 8).
  • SM medium Select shoots from SM medium are transferred to rooting medium (sucrose 30g / l, MS 4.43g / l, cefotaxime 250mg / l, PPT 3mg / l, pH5.8) and purified when roots are formed. After the process, they were transplanted into soil and transferred to the greenhouse. When the plants adapted to some extent, herbicide (0.1% bastard) resistance was confirmed.
  • rooting medium sucrose 30g / l, MS 4.43g / l, cefotaxime 250mg / l, PPT 3mg / l, pH5.8
  • Genomic DNA was isolated from the transgenic cabbage that is resistant to 0.1% BASTA to determine the presence of the BAR gene.
  • the specific 5 'primer (5'-ACAGCGACCACGCTGTTGAA-3': SEQ ID NO: 4) and 3 'primer (5') -TGCACCATCGTCAACCACTA-3 ': SEQ ID NO: 5) was assayed using PCR at 55 ° C 30 cycles, and the results are shown in FIG. 8.
  • lines # 1, # 13, # 27 and # 40 were PCR products were observed at the same position as the positive control, whereas non-transformers were no PCR products were observed, line # 1, # 13, # 27 and # 40 were found to be transformants.

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention se rapporte à un procédé d'augmentation de la résistance aux maladies de végétaux par la transformation de cellules végétales au moyen d'un vecteur de recombinaison comprenant le gène de la protéine de répétition Oryza sativa riche en leucine, dérivé d' Oryza sativa; à un végétal possédant une résistance aux maladies de végétaux accrue et transformé au moyen du vecteur de recombinaison et à une semence de celui-ci; à une composition pour augmenter la résistance aux maladies de végétaux, contenant le gène de la protéine de répétition Oryza sativa riche en leucine, dérivé d' Oryza sativa, et à un procédé d'augmentation de la productivité de végétaux par la transformation de cellules végétales au moyen du vecteur de recombinaison.
PCT/KR2010/007117 2009-10-19 2010-10-18 Gene oslrp pour augmenter la resistance au maladies de vegetaux et leur utilisation WO2011049334A2 (fr)

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KR10-2009-0099069 2009-10-19
KR1020090099069A KR101120458B1 (ko) 2009-10-19 2009-10-19 벼 줄무늬잎마름병에 대한 저항성을 증진시키는 유전자 및 이의 용도

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WO2011049334A3 WO2011049334A3 (fr) 2011-11-17

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CN102399274A (zh) * 2011-11-03 2012-04-04 浙江师范大学 负调控水稻抗病与细胞死亡的基因及其用途
CN102888399A (zh) * 2012-09-30 2013-01-23 浙江师范大学 鉴定水稻高抗白叶枯病基因的sts分子标记及其应用

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KR101612590B1 (ko) 2013-10-25 2016-04-15 경희대학교 산학협력단 증진된 병충해 저항성을 가지는 카페인 생합성 유전자가 도입된 형질전환 벼

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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CN102399274B (zh) * 2011-11-03 2013-07-31 浙江师范大学 负调控水稻抗病与细胞死亡的基因及其用途
CN102888399A (zh) * 2012-09-30 2013-01-23 浙江师范大学 鉴定水稻高抗白叶枯病基因的sts分子标记及其应用
CN102888399B (zh) * 2012-09-30 2014-02-05 浙江师范大学 鉴定水稻高抗白叶枯病基因的sts分子标记及其应用

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KR101120458B1 (ko) 2012-06-27
KR20110042409A (ko) 2011-04-27
WO2011049334A9 (fr) 2011-09-29
WO2011049334A3 (fr) 2011-11-17

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